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Polyethyleneimine |
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Vaxjo ID |
280 |
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Vaccine Adjuvant Name |
Polyethyleneimine |
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Adjuvant VO ID |
VO_0005768
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Description |
A virus-like particle (VLP) derived from bacteriophage Qβ, chemically conjugated with croconium near-infrared dyes. It serves as both a photothermal therapy agent and an immunological adjuvant, enhancing tumor ablation and immune activation upon NIR laser irradiation |
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Stage of Development |
Research |
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Host Species for Testing |
Mouse |
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Components |
Virus-like particles (VLPs) are multifunctional nanocarriers that mimic the architecture of viruses |
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Structure |
30 nm icosahedral VLP composed of 180 Qβ coat proteins Conjugated with ~212 croconium dye molecules per VLP Functionalized via NHS ester–lysine coupling |
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Appearance |
Dark blue-to-black colored solution or suspension (due to croconium dye) |
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Preparation |
Expression of Qβ in endotoxin-free E. coli Chemical synthesis of croconium dye NHS ester-based conjugation to Qβ Purification by ultracentrifugation and size-exclusion chromatography |
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Dosage |
Intratumoral injection: 50 μL of PTPhage solution Chromophore concentration: e.g., 30 μg/mL of dye NIR irradiation: 808 nm, 0.18 W/cm², 10 minutes |
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Function |
This system can generate more heat upon 808 nm NIR laser radiation than free dye and possesses a photothermal efficiency comparable to gold nanostructures, yet it is biodegradable and acts as an immunoadjuvant combined with the heat it produces. |
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Safety |
High biocompatibility and safety |
| References |
Jin et al., 2022: Jin Z, Dong YT, Liu S, Liu J, Qiu XR, Zhang Y, Zong H, Hou WT, Guo SY, Sun YF, Chen SM, Dong HQ, Li YY, An MM, Shen H. Potential of Polyethyleneimine as an Adjuvant To Prepare Long-Term and Potent Antifungal Nanovaccine. Frontiers in immunology. 2022; 13; 843684. [PubMed: 35651617].
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