PEI-PPAS |
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Vaxjo ID |
275 |
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Vaccine Adjuvant Name |
PEI-PPAS |
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Alternative Names |
Polyethyleneimine–Alhagi honey polysaccharide–PLGA Pickering emulsion system |
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Adjuvant VO ID |
VO_0005763
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|
Description |
A novel adjuvant consisting of PLGA nanoparticles encapsulating Alhagi honey polysaccharide (AHP), modified with PEI and emulsified with squalene, forming a Pickering emulsion with high antigen adsorption capacity and immunogenicity |
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Stage of Development |
Research |
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Host Species for Testing |
Chicken |
|
Components |
PLGA nanoparticles (PEI-AHPP) |
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Structure |
Spherical nano-emulsion with ~1000 nm particle size, strawberry-like rough surface to enhance antigen loading |
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Appearance |
Emulsion; rough-surfaced particles in aqueous media |
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Storage |
Freeze-dried at –20 °C; stable for up to 64 days at 37 °C without phase separation |
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Preparation |
<ul><li>PLGA nanoparticles with AHP prepared by double emulsion</li><li>Surface modified with PEI</li><li>Mixed with squalene oil phase via ultrasonication (80 W, 90 s)</li></ul> |
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Dosage |
0.2 mL per chicken (1:1 v/v ratio of PEI-PPAS:H9N2 antigen); intramuscular injection; 2-dose schedule |
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Function |
This study involved the preparation of the polyethyleneimine-modified immunopotentiator Alhagi honey polysaccharide encapsulated PLGA nanoparticles (PEI-AHPP) and the assembly of the Pickering emulsion with PEI-AHPP as shell and squalene as core (PEI-PPAS). |
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Safety |
No observable histopathological toxicity in spleen, thymus, or bursa of Fabricius; considered safe in animal models |
| References |
Wusiman et al., 2022: Wusiman A, Li J, Abulikemu X, Pengfei G, Mai Z, Jiang W, Liu Z, Wang D, Abula S, Guo Q. Polyethyleneimine modified Pickering emulsion as a novel adjuvant to induce strong and long-lasting immune responses. International journal of pharmaceutics. 2022; 619; 121713. [PubMed: 35367581].
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