solid-in-oil-in-water emulsion |
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Vaxjo ID |
269 |
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Vaccine Adjuvant Name |
solid-in-oil-in-water emulsion |
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Adjuvant VO ID |
VO_0005756
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Description |
A novel vaccine carrier where solid-state antigens are encapsulated inside the squalene oil phase of an oil-in-water emulsion, enhancing co-delivery with adjuvant and improving both humoral and cellular immunity. |
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Stage of Development |
Research |
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Host Species for Testing |
3 |
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Structure |
Oil-in-water emulsion with solid protein antigen particles in oil Oil phase: squalene, EPC, Span 85 Surfactant: Tween 80 |
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Appearance |
Emulsion; white solid dispersed in liquid (based on preparation description) |
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Preparation |
OVA mixed with EPC and Span85 in cyclohexane Freeze-dried to solid Dispersed in squalene (S/O nanodispersion) Emulsified with Tween80 in PBS → S/O/W emulsion Centrifugation purification |
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Dosage |
Mice received 200 µg/g OVA, 0.5 wt% of surfactants 100 µL/site subcutaneous injection, twice (day 0 and 7) |
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Function |
In the present study, a solid-in-oil-in-water (S/O/W) emulsion encapsulating antigen in the oil phase of an oil-in-water (O/W) emulsion was prepared as a novel vaccine carrier consisting of similar materials to the emulsion adjuvant of which the safety, immunogenicity and vaccination efficacy have been already confirmed in human. |
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Safety |
Good safety profile in mice |
| References |
Tahara et al., 2022: Tahara Y, Mizuno R, Nishimura T, Mukai SA, Wakabayashi R, Kamiya N, Akiyoshi K, Goto M. A solid-in-oil-in-water emulsion: An adjuvant-based immune-carrier enhances vaccine effect. Biomaterials. 2022; 282; 121385. [PubMed: 35093824].
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