TLR7-8 RNA ligands |
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Vaxjo ID |
247 |
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Vaccine Adjuvant Name |
TLR7-8 RNA ligands |
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Adjuvant VO ID |
VO_0005734
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Description |
Synthetic single-stranded RNA molecules designed to activate TLR7 and TLR8 in immune cells, promoting innate immune responses and co-stimulation of γδ T cells |
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Stage of Development |
Research |
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Components |
Here we have used recently described RNA ligands for TLR7 and TLR8 together with V纬9V未2 T-cell specific pyrophosphate antigens to analyze the rapid cytokine induction in V未2 T cells as well as the accessory cell requirements. While TLR8- as well as TLR7/8-specific RNA did not induce IFN-纬 in V未2 T cells on their own, they provided strong co-stimulation for V未2 T cells within peripheral blood mononuclear cells in the presence of additional T-cell receptor activation. In contrast, TLR7 ligands were ineffective. |
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Structure |
Short synthetic single-stranded RNA oligonucleotides, complexed with poly-arginine (pArg) for delivery |
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Preparation |
Short synthetic single-stranded RNA oligonucleotides, complexed with poly-arginine (pArg) for delivery |
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Dosage |
ssRNA40: 2 µg/mL R2152 & 9.2s: 4 µg/mL Optimal concentrations determined via titration for PBMC activation |
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Function |
Toll-like receptors (TLR) are pattern recognition receptors in innate immune cells which sense microbial structures including nucleic acids |
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Safety |
No adverse effects reported in vitro; well-tolerated in PBMC culture models |
| References |
Serrano et al., 2021: Serrano R, Coch C, Peters C, Hartmann G, Wesch D, Kabelitz D. Monocyte-dependent co-stimulation of cytokine induction in human γδ T cells by TLR8 RNA ligands. Scientific reports. 2021; 11(1); 15231. [PubMed: 34315922].
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