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LIGHT |
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Vaxjo ID |
211 |
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Vaccine Adjuvant Name |
LIGHT |
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Adjuvant VO ID |
VO_0005646
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Description |
LIGHT is a member of the TNF superfamily and can function as a costimulatory molecule for human naive T cells to proliferate. It can be a potential gene adjuvant. |
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Stage of Development |
Research |
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Host Species for Testing |
Mouse |
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Components |
LIGHT, a member of TNF superfamily, can function as a costimulatory molecule for human na?ve T cells to proliferate and can be a potential gene adjuvant. |
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Structure |
Eukaryotic expression plasmid pcDNA-nef was constructed by inserting a full-length nef gene into pcDNA3.1(+). LIGHT expression plasmids were also used. |
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Preparation |
The eukaryotic expression plasmid pcDNA-nef was constructed by inserting a full-length nef gene into pcDNA3.1(+). |
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Dosage |
BALB/c mice were immunized with HIV-1 nef DNA vaccine plasmids alone or in combination with LIGHT expression plasmids, but specific dosage amounts are not provided. |
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Function |
In this study, the eukaryotic expression plasmid pcDNA-nef was constructed by inserting the full-length nef gene into pcDNA3.1(+), and in vitro transfection confirmed successful nef expression in mammalian cells. BALB/c mice immunized with HIV-1 nef DNA vaccine plasmids, alone or combined with LIGHT expression plasmids, were evaluated for humoral and cellular immune responses. While the nef DNA vaccine induced anti-Nef antibodies, lymphocyte proliferation, and CTL activity, co-immunization with LIGHT plasmids significantly enhanced these responses. These results demonstrate that the nef-expressing vector elicits specific immunity against HIV-1 Nef, and LIGHT acts as a potent gene adjuvant for HIV-1 DNA vaccination. |
| References |
Wen et al., 2014: Wen J, Hao W, Fan Y, Du J, Du B, Qian M, Jiang W. Co-delivery of LIGHT expression plasmid enhances humoral and cellular immune responses to HIV-1 Nef in mice. Archives of virology. 2014; 159(7); 1663-1669. [PubMed: 24435162].
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