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Saponin Monophosphoryl Lipid-A Nanoparticles |
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Vaxjo ID |
191 |
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Vaccine Adjuvant Name |
Saponin Monophosphoryl Lipid-A Nanoparticles |
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Alternative Names |
SMNP |
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Adjuvant VO ID |
VO_0006095
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Description |
combination vaccine adjuvant that includes AS01-mimic, Immune Stimulating Complex (ISCOM) with a TLR4 agonist that induces a mixed Th1/Th2 response |
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Stage of Development |
Research |
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Location Licensed |
US (Massachusetts institute of technology) |
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Host Species for Testing |
Mouse |
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Second Host Species for Testing |
Macaque |
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Components |
QS-21 saponin and monophosphoryl lipid A and cholesterol and phospholipids |
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Structure |
~40 nm nanocages Molar ratio (Quil-A:Cholesterol:DPPC:MPLA) = 10:10:2.5:1 Physically incorporates MPLA into the nanoparticle |
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Storage |
4 C in liquid form or frozen at -20C in PBS pH 7.4 or 6.5 |
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Preparation |
solubilized in micelles of surfactant MEGA-10 then diluted below CMC, then self-assembled using QS-21 |
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Dosage |
Mice: 5 µg Quil-A per dose, corresponding to 0.5 µg MPLA NHPs: 375 µg SMNP per side (total 750 µg) |
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Function |
robust production of cytokines and chemokines in vaccine site-draining lymph nodes, enhances lymphatic transport of antigen, and increases antigen accumulation in draining lymph nodes |
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Safety |
safe in mice and NHPs |
| References |
(Michaels, 2023): Vaccine Adjuvant Compendium - VAC [https://vac.niaid.nih.gov/view?id=77]
Silva et al., 2021: Silva M, Kato Y, Melo MB, Phung I, Freeman BL, Li Z, Roh K, Van Wijnbergen JW, Watkins H, Enemuo CA, Hartwell BL, Chang JYH, Xiao S, Rodrigues KA, Cirelli KM, Li N, Haupt S, Aung A, Cossette B, Abraham W, Kataria S, Bastidas R, Bhiman J, Linde C, Bloom NI, Groschel B, Georgeson E, Phelps N, Thomas A, Bals J, Carnathan DG, Lingwood D, Burton DR, Alter G, Padera TP, Belcher AM, Schief WR, Silvestri G, Ruprecht RM, Crotty S, Irvine DJ. A particulate saponin/TLR agonist vaccine adjuvant alters lymph flow and modulates adaptive immunity. Science immunology. 2021; 6(66); eabf1152. [PubMed: 34860581].
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