VIOLIN: Vaccine Investigation and Online Information Network

Marek's disease virus

Table of Contents

Protective Antigens
1. MDV040
2. MDV095

I. Vaccine Related Protective Antigens

1. MDV040

Gene Name : MDV040
Sequence Strain (Species/Organism) : Gallid alphaherpesvirus 2
VO ID : VO_0011164
NCBI Gene ID : 4811501
NCBI Protein GI : 125745079
Locus Tag : MD5V_040
Genbank Accession : AF147806
Protein Accession : YP_001033956
Taxonomy ID : 10390
Gene Starting Position : 61024
Gene Ending Position : 63651
Gene Strand (Orientation) : -
Protein Name : envelope glycoprotein B
Protein pI : 8.4
Protein Weight : 92434.87
Protein Length : 865
Protein Note : glycoprotein BALF4; the Herpesviridae are non-segmented dsDNA viruses with genomes ranging from 120-230kbp; although herpes viruses vary greatly in sequence identity and homology, they all share four common elements: an envelope, a tegument which is composed of viral enzymes, a capsid of 162 capsomers, and a core composed of genomic DNA; BALF4 is a transmembrane glycoprotein found in the lipid envelope of several herpesviruses

DNA Sequence : Show Sequence

>NC_002229.3:61024-63651 Gallid herpesvirus 2, complete genome
TTTTATTCAGTTCAAATATAATAGTGCCCACTTACACAGCATCATCTTCTGAGTCTGAATATGTAGTAGG
TAACTTATCATATTTAGGGTTACTATTTTTAATCCTCATTTTTGCCAGGTGGTCCGATAGAACGGCGGTA
GTGCCTCGCCTCTTTCTCCGCAGTTTTTTCTCGTGGCGTTCTTCCGCGGAGACTAACGCCATATATTTTA
TCATTTCTCTAGCTTCTTCTAATTTTCTTTCATCAATAGATGTTCGTTCCAAATCATCTGATTCCTCGCC
ATGCAACTCACGCGTTGCCTGTGCCTTAAGCACTTCTGTTGTCATAGGATAAAGGGCTTTCATTGGATTG
CTTTTAAGCTTGTTTACATAACGATATGCTAAAAATGCAGCCACGAGTCCTGCTATAATGATTAAACCGA
TTGCCAAAGCCCCAAAGGGATTTGACATGAAAGCAGAGACACCAGATATGGTAGATACGATTGCACCGGC
AGCCCCTACTACAACTTTGCCTATAGCTTGCCCTACCTGACCCATACCGTTAAACAATTCGGCCAAACCG
TTCATAAACGCGTAATTTGTATCCACTTCTATTACTTTGTTTATGTCATAAAATTTAAGTTCATGTAGTT
GATTGCGGCGAGCTACTTCTGCATAATCCAATACACCAACATCACGCAACTCTTCTTTTGTGTAAACGGA
TAAAGGCAAAATTTCCCGATCTTCTAGCAGGGTTAGATTAAGCTCGACAAATGTGCTAGCAATCTGTATA
TCGGCAGCGTCTACCATCTTAACAAAATTATAGTTTTCAAATAAAGCATAACCGGATCCAAACAGAAAAT
ATCTACGATGATTAGCCGAGCATGGCTCTACAGCCTCTAGCGTTGGAAGCAACTCGTTGTTTTCACCGAG
TTGTCCCTGTATGTTTCCTTGGTTTTCTCCATATGAAAATAGAACCAATGGTCGGCTATAACATGTATTA
GTGGATGTGATAACTCGCATAGAATTTTGCAAAGTGACGGATTCCGCATCTATAGCAGTGCAGCTCGATA
CAGCAGCGACATCCCCCAACATCTTTGCAGCCACTCTCCTTCCTAATGTTGCACTCGCTGTAGCGCTAGG
ATTAATCTTTATCCCTTCGTGCCATAAAACAAGTTCTCTATTCTGCAATTCGCACCAAGCTGTGGCAATC
CTACTAAACATATCATTAATATGGGTTTGTATATGATCATAAAGAAATTGGAGCATGGCGAATTGAACAG
ACGATGTCGATTTAATAGCTGTGGTGTCGTCTAATGTTATTTTTCTATTTGGTGCATTTCGAATATCTCG
CCGCAATCGTGACAATGAGGTAGCATTTTTCTTATAAATTGCATGCTTATTGTTTACCAGGTCGAGCATC
TCATCGGTCCTGTTGTCTCTCATCAATTCTCTGAGGTACATATGAGCCAGGGATTTGGATAGAACAGGCT
GATATGCTACAATAAATCCCCCGAGAGCCAAGAAATATTGTACATGTCCAACCTTGACGTGACTGTCATT
ATATTTTGTCCTAAATATCTGCTCGATTGCTGCTTCTGCCTCGCGTTTAATACATTGTCCTAATATGATG
CGATTTGGATCAAACTCAGTCGTATTACTGATAAACGTTGCCGAAAGTTCACGGGCCATAAATCTGTATC
TCCCATTAACTGTTGCACGCAACATTTCAGTCACCTCTTTCCACTTAGTCATTGAACATACACGAGTAGT
TTTTGGAGCCCAGTCCCACCCAACTGTGAAGTGTGATGTGATGAGAAAGTTACGCTTGACTGGAAGGCTT
GCTTTTCGACGCTTGTCCAAATCCATTGAAAAATAGCTATCTAGTTGTTTGAAATTATCCTGGGGATATC
CCATGGGTTCTGCGGCAGCCTCTGGTGGGGATAGACCATAAAATGGAGATATGTTCGCGATGTCGCCATT
GGCCATTGCAAAATATGAATACGGAAACACAGAGCGGGCATCCATTTCCTCTACTATACAATTGACGGAG
GTTCCCGTTCGATATATCCATGGTGATCCCCACACGGTATACGTCTCATTAGTCGTGTGCCATGCCCTAG
ATTCGGGCGTGTTGAATTTTGATGGTTTTAGAAGTACTTGTTTTTCTCCCGCATCCCTGTCAAACGCTTC
AACATATACATTGTTTCTAAGGTATCTTGCTTTAGATGAGCATCTTCCTTTGCCGTCGATTAGATCCGTG
ATCTCTTCAATGGAAACGGGCGTCCTATCTGTATATCGATTAGTGATCTGTCTATATGTCGTCCCCGTCC
ATGTCGTCGTCTGAATGATATTTTTATAATAAAGCGTCACTTTAAATTTATATGGACTGATATTCTCTTT
AAATAATATCGCGATTCCTTCACCCCATTCGGTGGCTTTTCTAGGTTCGGGACATTTTCGCGGCGGTTCT
AGACGGATCACGGTTGAACCCACTGGTGGGGGACAAAGATAAAACGTAGACTCTTCCTCAGACAACTGGA
CGCTCGAAACAACTTCTCTTGATGTCACATTTTGGGTACTCGGAGATGAGTTCGTACCATATAGAATAAC
TATAAGGAAAAAAATGCAATTCCGCCTAAAATAGTGCA

Protein Sequence : Show Sequence

>YP_001033956.1 envelope glycoprotein B [Gallid alphaherpesvirus 2]
MHYFRRNCIFFLIVILYGTNSSPSTQNVTSREVVSSVQLSEEESTFYLCPPPVGSTVIRLEPPRKCPEPR
KATEWGEGIAILFKENISPYKFKVTLYYKNIIQTTTWTGTTYRQITNRYTDRTPVSIEEITDLIDGKGRC
SSKARYLRNNVYVEAFDRDAGEKQVLLKPSKFNTPESRAWHTTNETYTVWGSPWIYRTGTSVNCIVEEMD
ARSVFPYSYFAMANGDIANISPFYGLSPPEAAAEPMGYPQDNFKQLDSYFSMDLDKRRKASLPVKRNFLI
TSHFTVGWDWAPKTTRVCSMTKWKEVTEMLRATVNGRYRFMARELSATFISNTTEFDPNRIILGQCIKRE
AEAAIEQIFRTKYNDSHVKVGHVQYFLALGGFIVAYQPVLSKSLAHMYLRELMRDNRTDEMLDLVNNKHA
IYKKNATSLSRLRRDIRNAPNRKITLDDTTAIKSTSSVQFAMLQFLYDHIQTHINDMFSRIATAWCELQN
RELVLWHEGIKINPSATASATLGRRVAAKMLGDVAAVSSCTAIDAESVTLQNSMRVITSTNTCYSRPLVL
FSYGENQGNIQGQLGENNELLPTLEAVEPCSANHRRYFLFGSGYALFENYNFVKMVDAADIQIASTFVEL
NLTLLEDREILPLSVYTKEELRDVGVLDYAEVARRNQLHELKFYDINKVIEVDTNYAFMNGLAELFNGMG
QVGQAIGKVVVGAAGAIVSTISGVSAFMSNPFGALAIGLIIIAGLVAAFLAYRYVNKLKSNPMKALYPMT
TEVLKAQATRELHGEESDDLERTSIDERKLEEAREMIKYMALVSAEERHEKKLRRKRRGTTAVLSDHLAK
MRIKNSNPKYDKLPTTYSDSEDDAV

Molecule Role : Protective antigen
Molecule Role Annotation : Recombinant fowl poxviruses (rFPVs) were constructed to express genes from serotype 1 Marek's disease virus (MDV, Gallid herpesvirus 2). rFPV/gB1 expressing MDV040 conferred 42% protection in maternal antibody-positive chickens when challenged with highly virulent MDV isolates (Lee et al., 2003).
Related Vaccine(s): Marek's Disease Virus Vaccine rFPV/gB1

2. MDV095

Gene Name : MDV095
Sequence Strain (Species/Organism) : Gallid alphaherpesvirus 2
VO ID : VO_0011165
NCBI Gene ID : 4811454
NCBI Protein GI : 125745128
Locus Tag : MD5V_095
Genbank Accession : AF243438
Protein Accession : YP_001034012
Taxonomy ID : 10390
Gene Starting Position : 161182
Gene Ending Position : 164094
Gene Strand (Orientation) : +
Protein Name : envelope glycoprotein I
Protein pI : 6.7
Protein Weight : 37988.3
Protein Length : 355
Protein Note : envelope glycoprotein I; the Herpesviridae are non-segmented dsDNA viruses with genomes ranging from 120-230kbp; although herpes viruses vary greatly in sequence identity and homology, they all share four common elements: an envelope, a tegument which is composed of viral enzymes, a capsid of 162 capsomers, and a core composed of genomic DNA;virion envelope glycoproteins bind to cellular receptors; envelope glycoprotein E and glycoprotein I form a heterodimer that play important roles in virus cell-to-cell spread

DNA Sequence : Show Sequence

>NC_002229.3:161182-164094 Gallid herpesvirus 2, complete genome
GATGTATCTACTACAATTATTATTTTGGATCCGCCTCTTTCGAGGCATCTGGTCTATAGTTTATACTGGA
ACATCTGTTACGTTATCAACGGACCAATCTGCTCTTGTTGCGTTCTGCGGATTAGATAAAATGGTGAATG
TACGCGGCCAACTTTTATTCCTGGGCGACCAGACTCGGACCAGTTCTTATACAGGAACGACGGAAATCTT
GAAATGGGATGAAGAATATAAATGCTATTCCGTTCTACATGCGACATCATATATGGATTGTCCTGCTATA
GACGCCACGGTATTCAGAGGCTGTAGAGACGCTGTGGTATATGCTCAACCTCATGATAGAGTACAACCTT
TTCCCGAAAAGGGAACATTGTTGAGAATTGTCGAACCCAGAGTATCAGATACAGGCAGCTATTACATACG
TGTAGCTCTCGCTGGAAGAAATATGAGCGATATATTTAGAATGGCTGTTATTATAAGGAGTAGCAAATCT
TGGGCCTGTAATCACTCTGCTAGTTCATTTCAGGCCCATAAATGTATTCGCTATGTCGACCGTATGGCCT
TTGAAAATTATCTGATTGGACATGTAGGCAATTTGCTGGACAGTGACTCGGAATTGCATGCAATTTATAA
TATTACTCCCCAATCCATTTCCACAGATATTAATATTATAACGACTCCATTTTACGATAATTCGGGAACA
ATTTATTCACCTACGGTTTTTAATTTGTTTAATAACAATTCCCATGTCGATGCAATGAATTCGACTGGTA
TGTGGAATACCGTTTTAAAATATACCCTTCCAAGGCTTATTTACTTTTCTACGATGATTGTACTATGTAT
AATAGCATTGGCAATTTATTTGGTCTGTGAAAGGTGCCGCTCTCCCCATCGTAGGATATACATCGGTGAA
CCAAGATCTGATGAGGCCCCACTCATCACTTCTGCAGTTAACGAATCATTTCAATATGATTATAATGTAA
AGGAAACTCCTTCAGATGTTATTGAAAAGGAGTTGATGGAAAAACTGAAGAAGAAAGTCGAATTGTTGGA
AAGAGAAGAATGTGTATAGGTTTGAGAAACTATTATAGGTAGGTGGTACCTGTTAGCTTAGTATAAGGGG
AGGAGCCGTTTCTTGTTTTAAAGACACGAACACAAGGCAGTAAGTTTTATATGTGAATTTTGTGCATGTC
TGCGAGTCAGCGTCATAATGTGTGTTTTCCAAATCCTGATAATAGTGACGACGATCAAAGTAGCTGGAAC
GGCCAACATAAATCATATAGACGTTCCTGCAGGACATTCTGCTACAACGACGATCCCGCGATATCCACCA
GTTGTCGATGGGACCCTTTACACCGAGACGTGGACATGGATTCCCAATCACTGCAACGAAACGGCAACAG
GCTATGTATGTCTGGAAAGTGCTCACTGTTTTACCGATTTGATATTAGGAGTATCCTGCATGAGGTATGC
GGATGAAATCGTCTTACGAACTGATAAATTTATTGTCGATGCGGGATCCATTAAACAAATAGAATCGCTA
AGTCTGAATGGAGTTCCGAATATATTCCTATCTACGAAAGCAAGTAACAAGTTGGAGATACTAAATGCTA
GCCTACAAAATGCGGGTATCTACATTCGGTATTCTAGAAATGGGACGAGGACTGCAAAGCTGGATGTTGT
TGTGGTTGGCGTTTTGGGTCAAGCAAGGGATCGCCTACCCCAAATGTCCAGTCCTATGATCTCATCCCAC
GCCGATATCAAGTTGTCATTAAAAAACTTTAAAGCATTAGTATATCACGTGGGAGATACTATCAATGTCT
CGACGGCGGTTATACTAGGACCTTCTCCGGAGATATTCACATTGGAATTTAGGGTGTTGTTCCTCCGTTA
TAATCCAACGTGCAAGTTCGTCACGATTTATGAACCTTGTATATTTCACCCCAAAGAACCAGAGTGTATT
ACTACTGCAGAACAATCGGTATGTCATTTCGCATCCAACATTGACATTCTGCAGATAGCCGCCGCACGTT
CTGAAAATTGTAGCACAGGGTATCGTAGATGTATTTATGACACGGCTATCGATGAATCTGTGCAGGCCAG
ATTAACATTCATAGAACCAGGAATTCCTTCCTTTAAAATGAAAGATGTCCAGGTAGACGATGCTGGATTG
TATGTGGTTGTGGCTTTATACAATGGACGTCCAAGTGCATGGACTTACATTTATTTGTCAACGGTGGAAA
CATATCTTAATGTATATGAAAACTACCACAAGCCGGGATTTGGGTATAAATCATTTCTACAGAACAGTAG
TATCGTCGACGAAAATGAGGCTAGCGATTGGTCCAGCTCGTCCATTAAACGGAGAAATAATGGTACTATC
ATTTATGATATTTTACTCACATCGCTATCAATTGGGGCGATTATTATCGTCATAGTAGGGGGTGTTTGTA
TTGCCATATTAATTAGGCGTAGGAGACGACGTCGCACGAGGGGGTTATTCGATGAATATCCCAAATATAT
GACGCTACCAGGAAACGATCTGGGGGGCATGAATGTACCGTATGATAATACATGCTCTGGTAACCAAGTT
GAATATTATCAAGAAAAGTCGGCTAAAATGAAAAGAATGGGTTCGGGTTATACCGCTTGGCTAAAAAATG
ATATGCCGAAAATTAGGAAACGCTTAGATTTATACCACTGATATGTACATATTTAAACTTAATGGGATAT
AGTATATGGACGTCTATATGACGAGAGTAAATAAACTGACACTGCAAATGAAGCTGATCTATATTGTGCT
TTATATTGGGACAAACCACTCGCACAAGCTCATTCAACACATCCACTCTTGCTATTAAATTCCCCATTAT
ATAACAATACTGACATAACACTCATATTAAGGGGAGAAAATAA

Protein Sequence : Show Sequence

>YP_001034012.1 envelope glycoprotein I [Gallid alphaherpesvirus 2]
MYLLQLLFWIRLFRGIWSIVYTGTSVTLSTDQSALVAFCGLDKMVNVRGQLLFLGDQTRTSSYTGTTEIL
KWDEEYKCYSVLHATSYMDCPAIDATVFRGCRDAVVYAQPHDRVQPFPEKGTLLRIVEPRVSDTGSYYIR
VALAGRNMSDIFRMAVIIRSSKSWACNHSASSFQAHKCIRYVDRMAFENYLIGHVGNLLDSDSELHAIYN
ITPQSISTDINIITTPFYDNSGTIYSPTVFNLFNNNSHVDAMNSTGMWNTVLKYTLPRLIYFSTMIVLCI
IALAIYLVCERCRSPHRRIYIGEPRSDEAPLITSAVNESFQYDYNVKETPSDVIEKELMEKLKKKVELLE
REECV

Molecule Role : Protective antigen
Molecule Role Annotation : Recombinant fowl poxviruses (rFPVs) were constructed to express genes from serotype 1 Marek's disease virus (MDV, Gallid herpesvirus 2). rFPV/gI expressing MDV095 conferred 43% protection in maternal antibody-positive chickens when challenged with highly virulent MDV isolates (Lee et al., 2003).
Related Vaccine(s): Marek's Disease Virus Vaccine rFPV/gI

II. References

1. Lee et al., 2003: Lee LE, Witter RL, Reddy SM, Wu P, Yanagida N, Yoshida S. Protection and synergism by recombinant fowl pox vaccines expressing multiple genes from Marek's disease virus. Avian diseases. 2003; 47(3); 549-558. [PubMed: 14562881].