WD
General Information
Protegen ID
753
Sequence Strain (Species/Organism)
Leishmania amazonensis
VO ID
VO_0011290
Taxonomy ID
5659
Other Database IDs
CDD:225201
Molecule Role
Protective antigen
Molecule Role Annotation
DNA vaccination in BALB/c mice with the LAWD (for Leishmania antigenic WD protein) and IL-12 genes significantly delayed lesion development from challenge with Leishmania amazonensis , which correlated with a dramatic reduction in parasite burdens (Campbell et al. , 2004 ).
Related Vaccines(s)
L. amazonensis DNA vaccine encoding WD protein
References
Campbell et al. , 2004: Campbell K, Popov V, Soong L. Identification and molecular characterization of a gene encoding a protective Leishmania amazonensis Trp-Asp (WD) protein. Infection and immunity . 2004; 72(4); 2194-2202. [PubMed: 15039343 ].
Gene Information
Gene Name
WD
NCBI Nucleotide GI
38455440
DNA Sequence
>gi|38455440|gb|AY457171.1| Leishmania amazonensis antigenic WD protein mRNA, complete cds
CCCTCCCACTACCACGCAGCTTGTCATATCATACTGTGTTTCATTGTATGCATCCGGCGTGAGCAATTCT
TTGTGTCCTCAACCGCTCTTTCCTTTCGTCTTTCATCGCTGTGCTTCCTCTCCCCTCAGCGCCCCCCCCT
CTCCCTTCTCGCACACGCACGCACAGTGGCACAGATACATACAGAGGCACTCCTCTTCTCCCTACACTAT
CTTGCAGTGCCCTCTTCTTCCCCCGCCTCTTACTCTGCCCGTCCCTCTCCGACTCTCTTTCACGTTTCGC
AACACCAGTATCAACCATGGCCAAGGAGAAACTCGGCTTCAAGATCCAGGGGAAGACCGTCTCCTACGAG
GGTTACCCCGAGCGTCCGCCAAGGGACCTGATGGACATCTTGGTCAAAATGGGTGTCGGCGAAGCTGCCG
TGGTGACCACCTTTCTAGAAAATATTGAGAACCACCGGGAGTTCACCGACGAGCAGGTGATGGTGATGTA
CAACGAAGCAAAAATGACGCGCGAGAAGAAGGCTCGTGCGAAGGCCGGCAACCAGGGTGACTCAGCCGCT
GCCGCCGCCGCTCCATCGGGCGCCGCCGATGAGGCGGAGAAGCCGCAGAAGATCCTTCTTTCCTACCGCG
GCAAGCAGCTTTCCTACGTAGGTTTGCCCAGCGGCAAGCGCAACGCCGTGCTGCAACTCCTCTCCAAGCA
AGAGGAGCTCACCCGGGAGCTTGCAGTGGAGACGTTCCGCACGAACCTGCCCAGTGATCCGTCCACGGAG
GAGGAGATTTGGATCTTGGTGCAGGCAGTCAAAGATCGCAAGAGTAGGAAGAGCCAAGCGAAGGGTCAGG
ATGGCGAAAGGTCCGGCGGTAACGGTGCGACTGCGTCGCTTGGGGCAGGCAGCAGCGCCATGGACAGCAT
AACCGGTATGTCCGAGAGGGAGCGCGACGACCTGAACAACTTCATTCTTCAGGTGCTGCAGCAGCCAGCG
CCGGATGTGGCCGCCCTCTTACATGAGAATCCGAAACCGCGCAAAGACACGGAAGACAATGCGGAGCTGG
CACTGACAGCGCGCATGTCGGGTAAGATTCCGGTTTACCTGCAGGAAGAGAACACCAGCAGCACGAAGCT
CATCTACGCCACGCCCCACATGAGCTTCGAAGATTTTTCTACCATTGTCGAGAAGAAGTGTGGCCGCAAG
TTGTCGCTGTCCTTCTACGACGGCGACGACCTCATCATGATGGATGATGACGACGTGCTGGCCATGTTTC
TCGAAATGACAGTGCAGAACGACCCAAAGAAGGTGCGCCTCATTTGCTCCAACCCTGGAGTCCCAAGGAA
GGCGGCGGATGACCGGATGATGGAAAACCGTCAAAATACCTTCAGCGGTCTTCCGCAGGTCTCAACGACC
AAGGCATTGGCGTACTCGAACGGCGAGCTCAACGTCGCGGAGGCTCGCACCTACTTTGGCCACTCTCTTG
CCGTCTACTGCTGCTGCTTCTCGCCAAGGGGCGACATGTTCGTTACGGCAAGTCGCGACCGCACCGTCCG
CCTGTGGAACTTGCGCACCGGTGTCTCCACAGTCATGAAAGGCGGGCACAACGGCTTTGTGCTGTCTTGC
GACTACTCGCCAAAGGGCAACCGCGTCGCTTCCTCTTCTGATGACCGCACTATCAAACTGTGGAACACGT
CGTCATGCAACAAGGTTGCCACACTTAAAGGGCACGAGGACAAGGTATACTGCGTCAAGTACAACTCTAG
CGGAGATCTCCTCGTGTCCGCCTCTTGCGACACCACAGTGCGCGTGTGGAACGCCGAAAGTCAGGCGAAG
CTGGTGACGCTGAGGGGCCACACCCTCGCCGTTTTTTCCTGCGCCTTCAGCAACAGCGATAATGGAAAGT
TCGTCGTCTCCGGCAGCGATGACCGCGTTATAAAGCTGTGGGACTGGGGCGCCGGCAGAGAGATCCTGTC
GCTGGTGGGTCACATCGGCACCGTGTGGACGGTTGTCTTCTCCCACAACGACAGGTACGTTCTCAGTGGC
AGCATGGACTATGAGCTCATTCTCTGGGATTCAATGACTGGCGCTCGCCTGCGCTCGATGGACGGGCACA
AGACATCGGTGCACCACGCCATCTTCTCCGAGGACGACGCATACATCTTCTCCTGCGCCCGCGACTGGAG
CGTCATGGTGTGGCGCACCGAAGATGGCGAGCATGTAGAGACCATCATCGGTCACCTCAGCACTGTCTAC
CACATGGACATCAGCGAGGACAAGTTGCTCACCTGCTCTCTTGATGACAAGATCAAGCTGTGGAATATCA
GGCGCCACTGAAGTTGAGCACCAGCGGAAAATGTGGTAGTGCGAACACTGGCGGGAGGGGCACGTGCCAG
CCGCAACCGAGAAAGAGGTGTGGGCGGGAAGGCACGTGATAGGTGATACAGACACTGTACCCGTACACCA
TTCCTCTTTTCAGCAGCAGTCCTGCACACAGAAAGACACTCGGATTTTTCATTGACAAATCAGGTGGGTC
TGAAGACGATTTCAAGAAGAAGCGAGCGTTCGTATGTACCGCCCTCCTCCCTCCACCGATACTGCAACAC
ACACACACACACATTTTACGGTCTTGTCTACTTTTCTTCGTTTCGCTCTCGTCAGTTCCACCGAGTCCTG
GCTGCGTTTGGGTGCCACGCGTGTGAATGTGTGAGCGCCACCGTTGGTCGACCCTTTTCGCTTTCCTGCA
TGCTTTTTTTCTTTTCGTATCTCTACGTCCTGCTCACAGCGCATCGACCTCTGCCAGAGGATATCATTTG
GCAGTGTCGTTTCTTTTGCTCTTTTTCTCTCTTTGCCACCGTTCTTGCGGGCCAACGGCACCGTGCTCCC
CGCTTCTCTGTCTCGCGACCTCGACATTCATCTCTTCCATGCTTCTCCCTCTCCCCCTCCGTCTCCCCAG
CTTTTTGTCGTTTGTGCATTCTCCTCTTTTCTTAAGTGATGCTTCAAAGGAAACGAAAAAAAAAAAAAAA
AA
Protein Information
Protein Name
antigenic WD protein
NCBI Protein GI
38455441
Protein Accession
AAR20840.1
Protein pI
6.52
Protein Weight
70626.83
Protein Length
743
Protein Note
WD40 repeat [General function prediction only]; COG2319
Protein Sequence
>AAR20840.1 antigenic WD protein [Leishmania amazonensis]
MAKEKLGFKIQGKTVSYEGYPERPPRDLMDILVKMGVGEAAVVTTFLENIENHREFTDEQVMVMYNEAKM
TREKKARAKAGNQGDSAAAAAAPSGAADEAEKPQKILLSYRGKQLSYVGLPSGKRNAVLQLLSKQEELTR
ELAVETFRTNLPSDPSTEEEIWILVQAVKDRKSRKSQAKGQDGERSGGNGATASLGAGSSAMDSITGMSE
RERDDLNNFILQVLQQPAPDVAALLHENPKPRKDTEDNAELALTARMSGKIPVYLQEENTSSTKLIYATP
HMSFEDFSTIVEKKCGRKLSLSFYDGDDLIMMDDDDVLAMFLEMTVQNDPKKVRLICSNPGVPRKAADDR
MMENRQNTFSGLPQVSTTKALAYSNGELNVAEARTYFGHSLAVYCCCFSPRGDMFVTASRDRTVRLWNLR
TGVSTVMKGGHNGFVLSCDYSPKGNRVASSSDDRTIKLWNTSSCNKVATLKGHEDKVYCVKYNSSGDLLV
SASCDTTVRVWNAESQAKLVTLRGHTLAVFSCAFSNSDNGKFVVSGSDDRVIKLWDWGAGREILSLVGHI
GTVWTVVFSHNDRYVLSGSMDYELILWDSMTGARLRSMDGHKTSVHHAIFSEDDAYIFSCARDWSVMVWR
TEDGEHVETIIGHLSTVYHMDISEDKLLTCSLDDKIKLWNIRRH
Epitope Information
IEDB Linear Epitope
-- Assay Type --
T Cell Epitope
B Cell Epitope
IEDB ID
Epitope
MHC restriction
Starting position
Ending position
IEDB ID
Epitope
Starting position
Ending position
MAKEKLGFKIQGKTVSYEGYPERPPRDLMDILVKMGVGEAAVVTTFLENIENHREFTDEQVMVMYNEAKMTREKKARAKAGNQGDSAAAAAAPSGAADEAEKPQKILLSYRGKQLSYVGLPSGKRNAVLQLLSKQEELTRELAVETFRTNLPSDPSTEEEIWILVQAVKDRKSRKSQAKGQDGERSGGNGATASLGAGSSAMDSITGMSERERDDLNNFILQVLQQPAPDVAALLHENPKPRKDTEDNAELALTARMSGKIPVYLQEENTSSTKLIYATPHMSFEDFSTIVEKKCGRKLSLSFYDGDDLIMMDDDDVLAMFLEMTVQNDPKKVRLICSNPGVPRKAADDRMMENRQNTFSGLPQVSTTKALAYSNGELNVAEARTYFGHSLAVYCCCFSPRGDMFVTASRDRTVRLWNLRTGVSTVMKGGHNGFVLSCDYSPKGNRVASSSDDRTIKLWNTSSCNKVATLKGHEDKVYCVKYNSSGDLLVSASCDTTVRVWNAESQAKLVTLRGHTLAVFSCAFSNSDNGKFVVSGSDDRVIKLWDWGAGREILSLVGHIGTVWTVVFSHNDRYVLSGSMDYELILWDSMTGARLRSMDGHKTSVHHAIFSEDDAYIFSCARDWSVMVWRTEDGEHVETIIGHLSTVYHMDISEDKLLTCSLDDKIKLWNIRRH
