VIOLIN: Vaccine Investigation and Online Information Network

Fnz

General Information

Protegen ID

630

Sequence Strain (Species/Organism)

Streptococcus equi subsp. zooepidemicus

VO ID

VO_0011214

Taxonomy ID

40041

Molecule Role

Protective antigen

Molecule Role Annotation

Parts of the three recombinant proteins (FNZ (cell surface-bound fibronectin binding protein), SFS (secreted fibronectin binding protein), and EAG (alpha2-macroglobulin, albumin, and immunoglobulin G [IgG] binding protein)) were used to immunize mice, either subcutaneously or intranasally, prior to nasal challenge with S. equi subsp. equi. The adjuvant used was EtxB, a recombinant form of the B subunit of Escherichia coli heat-labile enterotoxin. It was shown that nasal colonization of S. equi subsp. equi and weight loss due to infection were significantly reduced after vaccination compared with a mock-vaccinated control group. This effect was more pronounced after intranasal vaccination than after subcutaneous vaccination; nearly complete eradication of nasal colonization was obtained after intranasal vaccination (P < 0.001) (Flock et al., 2004).

Related Vaccines(s)

S. equi Subunit EAG, FNZ and SFS Protein Vaccine

References

Flock et al., 2004: Flock M, Jacobsson K, Frykberg L, Hirst TR, Franklin A, Guss B, Flock JI. Recombinant Streptococcus equi proteins protect mice in challenge experiments and induce immune response in horses. Infection and immunity. 2004; 72(6); 3228-3236. [PubMed: 15155624].

Gene Information

Gene Name

Fnz

NCBI Gene ID

7695217

Genbank Accession

FM204884

Locus Tag

SZO_RS08670

Gene Starting Position

1860617

Gene Ending Position

1862326

DNA Sequence

>NC_012470.1:1860617-1862326 Streptococcus equi subsp. zooepidemicus H70, complete genome
TATGGAGAGAAGAGAGTTGAAAACAAAATCATTTAGAAAGGTACTGACGACGTCAGCTACCTGTATTGTG
CTGGCAACAAGCTTTGCCGGAGGAACCCTACGCGTCTGGGCAGAGCAGCTTTATTATGGGTGGGATGATG
GAACGAGACATGATTCACCCTCCTTTTTATATGTAACGCCTACTACTGAAAGTCAAAGAAGCCCCAAAAC
TATTGTATACTGTTTTAATCGAAACAATTATTGGCCGGATAATTGGGAAACCCATGTCTTAAAACCAGAT
AATCTACCTACTTATAATAAATTAACAGGAACTGCGAAGTTATTTCAAAATAAAGCTACCAAACCAAGAT
CGGTAGCAGATATCGAGAAGTCCTTAGTTTCTGTCCTAAGTAAAGGGTATCCAACAAGTAATCTCTCTCA
ATTTGGCATCAGTAATGAGTCAGCTAGAATAGTGACACAATTAGCCATTTGGTATTTTAGTGATAGTGTG
AACGATCCTGAAAGCGTACTTAGACAAAGATTAGGGGGCAATGAATCTAAAGCATTAAAATACCTTATTG
AGGTTGGTGAAAAACCACAATTGAGCGAAGCTGAGCAAAAGCAAACGTTAGATATATACGTTTACAACTC
AGGAGGTTATCGTAGAACAGGTTACCAAAATCTTCTCGGCTCTACCTTAATTCCTAAAACACCAGAAAAG
CCTTCTTCTGAATCTGTAACTCAGAGTAACCTTATTCTACTTGAACAGGAGAATCATTCTGGTACTATTT
CAGGTGGTTCAGGAAATACTGAAACTGAGGAGAATACTCCTCATCTAATGGGGATAGGTGGCGGTCTAGC
TGGTGAATCAGGAGAAAACACACCTAAACCAGGGGAAACTGGTGGGCACTCCCCTATAGTTGAAGAAAAC
TACGGTAGTACTGAAGAATTTCATGGGAATTCAGAAATTATTAGTGAAACCGAAGATACTAATCCTGGTA
TCATTTTAGGTGGCTCAGGAAATGTCGAAACACATGAAGATACTAGGAATCCTCATCTGATGGGGCTTGG
CGGTGGTCTAGCTGGCGAATCAGGAGAAACGACACCTAAACCAGGACAGACTGGTGGTCATGGACCAATC
ATCGAGACAACAGAGGACACACAAAAAGGCATGTCTGGGCAATCTGGCGGCACTATCGAGTCAGAAAACA
CCAAAAAGCCTGAGGTCATGATTGGTGGGCAGGGACAAACCATCGAAACAACAGAGGACACACAAAAAGG
CATGTCTGGGCAATCTGGCGGTACTATCGAGTCAGAAGACACTAAAAAACCTGAGGTCATGATTGGCGGT
CAGGGACAAATCATCGACTTCTCTGAAAACACGCAATCAGGTATGTCTGGACAGTCTGGAGACACTACGG
TCATTGAGGATACCAAGAAGTCTGAGATAATCATTGGTGGGCAAGGACAAATCATCGACTTCTCTGAGGA
TACTCAGCCGGGTATGTCTGGGCAATCTGGAGGCACCACAATTGTCGAAGACACCAAGAAGCCGACACCT
AAGCCTAAACCTGCACCTGCGCCAATTGTTAATGACGAAAAACCTAACAAAGGCACTCAGCTCCCACAGA
CAAATGATATGAAGCAACTCACCCTAAGCATCATCGGTGCAATGTCAATGCTGCTTGTCCTATGTCTGTC
TCTATTCAAGCGACCATCTAAAAAAGACTA

Protein Information

Protein Name

thioester-forming surface-anchored protein

NCBI Protein GI

754170878

Protein Accession

WP_041790586

Protein pI

4.75

Protein Weight

58938.07

Protein Length

569

Protein Note

Ortholog of S. equi 4047 (FM204883) SEQ0375

Protein Sequence

>WP_041790586.1 thioester-forming surface-anchored protein [Streptococcus equi]
MERRELKTKSFRKVLTTSATCIVLATSFAGGTLRVWAEQLYYGWDDGTRHDSPSFLYVTPTTESQRSPKT
IVYCFNRNNYWPDNWETHVLKPDNLPTYNKLTGTAKLFQNKATKPRSVADIEKSLVSVLSKGYPTSNLSQ
FGISNESARIVTQLAIWYFSDSVNDPESVLRQRLGGNESKALKYLIEVGEKPQLSEAEQKQTLDIYVYNS
GGYRRTGYQNLLGSTLIPKTPEKPSSESVTQSNLILLEQENHSGTISGGSGNTETEENTPHLMGIGGGLA
GESGENTPKPGETGGHSPIVEENYGSTEEFHGNSEIISETEDTNPGIILGGSGNVETHEDTRNPHLMGLG
GGLAGESGETTPKPGQTGGHGPIIETTEDTQKGMSGQSGGTIESENTKKPEVMIGGQGQTIETTEDTQKG
MSGQSGGTIESEDTKKPEVMIGGQGQIIDFSENTQSGMSGQSGDTTVIEDTKKSEIIIGGQGQIIDFSED
TQPGMSGQSGGTTIVEDTKKPTPKPKPAPAPIVNDEKPNKGTQLPQTNDMKQLTLSIIGAMSMLLVLCLS
LFKRPSKKD

Epitope Information

IEDB Linear Epitope