VIOLIN: Vaccine Investigation and Online Information Network

CWP2

General Information

Protegen ID

479

Sequence Strain (Species/Organism)

Giardia lamblia ATCC 50803

VO ID

VO_0011080

Taxonomy ID

50803

Molecule Role

Protective antigen

Molecule Role Annotation

The gene encoding the full length cyst wall protein-2 (CWP2) from Giardia lamblia was subcloned into the pCDNA3 mammalian expression vector and stably introduced into S. typhimurium STM1. Challenge infection with live Giardia muris cysts revealed that mice receiving the CWP2-encoding DNA vaccine were able to reduce cyst shedding by approximately 60% compared to control mice (Abdul-Wahid and Faubert, 2007).

Related Vaccines(s)

G. duodenalis DNA vaccine encoding CWP2

References

Abdul-Wahid and Faubert, 2007: Abdul-Wahid A, Faubert G. Mucosal delivery of a transmission-blocking DNA vaccine encoding Giardia lamblia CWP2 by Salmonella typhimurium bactofection vehicle. Vaccine. 2007; 25(50); 8372-8383. [PubMed: 17996337].

Gene Information

Gene Name

CWP2

NCBI Gene ID

5703171

Genbank Accession

AACB02000001

Locus Tag

GL50803_5435

Gene Starting Position

1061836

Gene Ending Position

1062924

DNA Sequence

>NW_002477099.1:1061836-1062924 Giardia lamblia ATCC 50803 SC_592, whole genome shotgun sequence
TTCACCTTCTGCGGACAATAGGCTTGTTCGTGCTGCGAGGCAGAGCTCTCACGGGTGCACGCTTCGATTG
GGTCGGCTTGGTGATCGCACGAGCGGTGGGGCGGGCTACAGCAGAGCGGCTAGCATTGCCAGTCACAGAG
CTCCTCGATTGGACCGTCTTCTTGGGAGCCGTCCTGGAGCGGGACGTTGAGGCCGTCTTGCTAGATGACC
TAACCACTGTCCTGAGCGGTGTTTGGCTGCGGCTGTTTGGCATGTTGCATTTCCTCTGCGCATTGTGTGC
ACTGTTGTGCATGTTGCTCTTGGCCTTGCCACAGTTGGAGGCGCTACGAGCGTTGCAGCTTGTCTTTCGT
CCCGACGCATTGCGGACTACTGTCTGCCTGTAGTACGTGCATCCATCTCTCTCGAGAGTTGTGGGGCAGT
TTGTCGGCGGGAGAGCAGTGCAGTAATCGCAGTCTGTTGTTCCACAGAGGTAGATTCCGGTGTAGGTGTC
AGGCGCAGCTGTGCAAACGAGATCGGTGTTGCACTGGACATGAATCTCCATCATGTAAGGCAGGTCGAAG
AGATCTGCCGGAACGTCGCCAGTCAGCTCGTTGCAGTCAAGGTGGAGCTCCTTGAGGAATTGGAGTTCGT
TGACGCAGGTGGGGATGGGACCAGTGAGAGCATTAATACTCATGTACCAAAACATCATGTGGGTTAAATC
ACAGATGCACTCGGGAATGTCACCCGTCAGCCCAGCGTTGTTAATTTGCAGGTACTGCATTGAGGTGAGG
GCACAAAGATCCGTCGGGATCGGGCCCGCAAGGTCATTATTATTAAGGTACAGCGACCTCAAAAGTGGGA
AGCAGCCTATGTCGGCAGGGAGGGCACCAGTGAGCCCCATGTCAGAGAGGTCGATCCCAATAACGTTATT
GTTAGAGTCGCATGTGATGCCGGTCCAGCTACAGTAAGACACGTCGGGCGTAAGCCAGTTATTCGACTTC
CAATTAGCACCATCCAGGGCATCGTACAAATTGGTCAGGGCCTCCTCCTCGGTGGCAGGGCAAGCGGCGC
GAGCAAGACCAAGAAGTCCTAGAACAAGGGCTGCGATCA

Protein Information

NCBI Protein GI

159120052

Protein Accession

XP_001710242

Protein pI

8.13

Protein Weight

37700.21

Protein Length

362

Protein Sequence

>XP_001710242.1 Cyst wall protein 2 [Giardia lamblia ATCC 50803]
MIAALVLGLLGLARAACPATEEEALTNLYDALDGANWKSNNWLTPDVSYCSWTGITCDSNNNVIGIDLSD
MGLTGALPADIGCFPLLRSLYLNNNDLAGPIPTDLCALTSMQYLQINNAGLTGDIPECICDLTHMMFWYM
SINALTGPIPTCVNELQFLKELHLDCNELTGDVPADLFDLPYMMEIHVQCNTDLVCTAAPDTYTGIYLCG
TTDCDYCTALPPTNCPTTLERDGCTYYRQTVVRNASGRKTSCNARSASNCGKAKSNMHNSAHNAQRKCNM
PNSRSQTPLRTVVRSSSKTASTSRSRTAPKKTVQSRSSVTGNASRSAVARPTARAITKPTQSKRAPVRAL
PRSTNKPIVRRR

Epitope Information

IEDB Linear Epitope