VIOLIN: Vaccine Investigation and Online Information Network

BoNT/A1 from C. botulinum A str. Hall

General Information

Protegen ID

448

Sequence Strain (Species/Organism)

Clostridium botulinum A str. Hall

VO ID

VO_0010903

Taxonomy ID

441771

Molecule Role

Protective antigen

Molecule Role Annotation

Mice and rabbits were immunized with recombinant HCR/A1 (rHCR/A1) from the classical type A-Hall strain (ATCC 3502) (BoNT/A1) and rHCR/E from BoNT serotype E Beluga (BoNT/E(B)). The protection elicited by rHCR/A1 to BoNT/A1 and BoNT/A2 and by rHCR/E(B) to BoNT/E(A) indicate that immunization with receptor binding domains elicit protection within sub-serotypes of BoNT (Baldwin et al., 2005).

Related Vaccines(s)

C. botulinum DNA vaccine pSCARSA/BHc

References

Baldwin et al., 2005: Baldwin MR, Tepp WH, Pier CL, Bradshaw M, Ho M, Wilson BA, Fritz RB, Johnson EA, Barbieri JT. Characterization of the antibody response to the receptor binding domain of botulinum neurotoxin serotypes A and E. Infection and immunity. 2005; 73(10); 6998-7005. [PubMed: 16177380].

Gene Information

Gene Name

BoNT/A1 from C. botulinum A str. Hall

NCBI Gene ID

5398487

Genbank Accession

CP000727

Locus Tag

CLC_0862

Gene Starting Position

882202

Gene Ending Position

886092

DNA Sequence

>NC_009698.1:882202-886092 Clostridium botulinum A str. Hall chromosome, complete genome
TATGCCATTTGTTAATAAACAATTTAATTATAAAGATCCTGTAAATGGTGTTGATATTGCTTATATAAAA
ATTCCAAATGCAGGACAAATGCAACCAGTAAAAGCTTTTAAAATTCATAATAAAATATGGGTTATTCCAG
AAAGAGATACATTTACAAATCCTGAAGAAGGAGATTTAAATCCACCACCAGAAGCAAAACAAGTTCCAGT
TTCATATTATGATTCAACATATTTAAGTACAGATAATGAAAAAGATAATTATTTAAAGGGAGTTACAAAA
TTATTTGAGAGAATTTATTCAACTGATCTTGGAAGAATGTTGTTAACATCAATAGTAAGGGGAATACCAT
TTTGGGGTGGAAGTACAATAGATACAGAATTAAAAGTTATTGATACTAATTGTATTAATGTGATACAACC
AGATGGTAGTTATAGATCAGAAGAACTTAATCTAGTAATAATAGGACCCTCAGCTGATATTATACAGTTT
GAATGTAAAAGCTTTGGACATGAAGTTTTGAATCTTACGCGAAATGGTTATGGCTCTACTCAATACATTA
GATTTAGCCCAGATTTTACATTTGGTTTTGAGGAGTCACTTGAAGTTGATACAAATCCTCTTTTAGGTGC
AGGCAAATTTGCTACAGATCCAGCAGTAACATTAGCACATGAACTTATACATGCTGGACATAGATTATAT
GGAATAGCAATTAATCCAAATAGGGTTTTTAAAGTAAATACTAATGCCTATTATGAAATGAGTGGGTTAG
AAGTAAGCTTTGAGGAACTTAGAACATTTGGGGGACATGATGCAAAGTTTATAGATAGTTTACAGGAAAA
CGAATTTCGTCTATATTATTATAATAAGTTTAAAGATATAGCAAGTACACTTAATAAAGCTAAATCAATA
GTAGGTACTACTGCTTCATTACAGTATATGAAAAATGTTTTTAAAGAGAAATATCTCCTATCTGAAGATA
CATCTGGAAAATTTTCGGTAGATAAATTAAAATTTGATAAGTTATACAAAATGTTAACAGAGATTTACAC
AGAGGATAATTTTGTTAAGTTTTTTAAAGTACTTAACAGAAAAACATATTTGAATTTTGATAAAGCCGTA
TTTAAGATAAATATAGTACCTAAGGTAAATTACACAATATATGATGGATTTAATTTAAGAAATACAAATT
TAGCAGCAAACTTTAATGGTCAAAATACAGAAATTAATAATATGAATTTTACTAAACTAAAAAATTTTAC
TGGATTGTTTGAATTTTATAAGTTGCTATGTGTAAGAGGGATAATAACTTCTAAAACTAAATCATTAGAT
AAAGGATACAATAAGGCATTAAATGATTTATGTATCAAAGTTAATAATTGGGACTTGTTTTTTAGTCCTT
CAGAAGATAATTTTACTAATGATCTAAATAAAGGAGAAGAAATTACATCTGATACTAATATAGAAGCAGC
AGAAGAAAATATTAGTTTAGATTTAATACAACAATATTATTTAACCTTTAATTTTGATAATGAACCTGAA
AATATTTCAATAGAAAATCTTTCAAGTGACATTATAGGCCAATTAGAACTTATGCCTAATATAGAAAGAT
TTCCTAATGGAAAAAAGTATGAGTTAGATAAATATACTATGTTCCATTATCTTCGTGCTCAAGAATTTGA
ACATGGTAAATCTAGGATTGCTTTAACAAATTCTGTTAACGAAGCATTATTAAATCCTAGTCGTGTTTAT
ACATTTTTTTCTTCAGACTATGTAAAGAAAGTTAATAAAGCTACGGAGGCAGCTATGTTTTTAGGCTGGG
TAGAACAATTAGTATATGATTTTACCGATGAAACTAGCGAAGTAAGTACTACGGATAAAATTGCGGATAT
AACTATAATTATTCCATATATAGGACCTGCTTTAAATATAGGTAATATGTTATATAAAGATGATTTTGTA
GGTGCTTTAATATTTTCAGGAGCTGTTATTCTGTTAGAATTTATACCAGAGATTGCAATACCTGTATTAG
GTACTTTTGCACTTGTATCATATATTGCGAATAAGGTTCTAACCGTTCAAACAATAGATAATGCTTTAAG
TAAAAGAAATGAAAAATGGGATGAGGTCTATAAATATATAGTAACAAATTGGTTAGCAAAGGTTAATACA
CAGATTGATCTAATAAGAAAAAAAATGAAAGAAGCTTTAGAAAATCAAGCAGAAGCAACAAAGGCTATAA
TAAACTATCAGTATAATCAATATACTGAGGAAGAGAAAAATAATATTAATTTTAATATTGATGATTTAAG
TTCGAAACTTAATGAGTCTATAAATAAAGCTATGATTAATATAAATAAATTTTTGAATCAATGCTCTGTT
TCATATTTAATGAATTCTATGATCCCTTATGGTGTTAAACGGTTAGAAGATTTTGATGCTAGTCTTAAAG
ATGCATTATTAAAGTATATATATGATAATAGAGGAACTTTAATTGGTCAAGTAGATAGATTAAAAGATAA
AGTTAATAATACACTTAGTACAGATATACCTTTTCAGCTTTCCAAATACGTAGATAATCAAAGATTATTA
TCTACATTTACTGAATATATTAAGAATATTATTAATACTTCTATATTGAATTTAAGATATGAAAGTAATC
ATTTAATAGACTTATCTAGGTATGCATCAAAAATAAATATTGGTAGTAAAGTAAATTTTGATCCAATAGA
TAAAAATCAAATTCAATTATTTAATTTAGAAAGTAGTAAAATTGAGGTAATTTTAAAAAATGCTATTGTA
TATAATAGTATGTATGAAAATTTTAGTACTAGCTTTTGGATAAGAATTCCTAAGTATTTTAACAGTATAA
GTCTAAATAATGAATATACAATAATAAATTGTATGGAAAATAATTCAGGATGGAAAGTATCACTTAATTA
TGGTGAAATAATCTGGACTTTACAGGATACTCAGGAAATAAAACAAAGAGTAGTTTTTAAATACAGTCAA
ATGATTAATATATCAGATTATATAAACAGATGGATTTTTGTAACTATCACTAATAATAGATTAAATAACT
CTAAAATTTATATAAATGGAAGATTAATAGATCAAAAACCAATTTCAAATTTAGGTAATATTCATGCTAG
TAATAATATAATGTTTAAATTAGATGGTTGTAGAGATACACATAGATATATTTGGATAAAATATTTTAAT
CTTTTTGATAAGGAATTAAATGAAAAAGAAATCAAAGATTTATATGATAATCAATCAAATTCAGGTATTT
TAAAAGACTTTTGGGGTGATTATTTACAATATGATAAACCATACTATATGTTAAATTTATATGATCCAAA
TAAATATGTCGATGTAAATAATGTAGGTATTAGAGGTTATATGTATCTTAAAGGGCCTAGAGGTAGCGTA
ATGACTACAAACATTTATTTAAATTCAAGTTTGTATAGGGGGACAAAATTTATTATAAAAAAATATGCTT
CTGGAAATAAAGATAATATTGTTAGAAATAATGATCGTGTATATATTAATGTAGTAGTTAAAAATAAAGA
ATATAGGTTAGCTACTAATGCATCACAGGCAGGCGTAGAAAAAATACTAAGTGCATTAGAAATACCTGAT
GTAGGAAATCTAAGTCAAGTAGTAGTAATGAAGTCAAAAAATGATCAAGGAATAACAAATAAATGCAAAA
TGAATTTACAAGATAATAATGGGAATGATATAGGCTTTATAGGATTTCATCAGTTTAATAATATAGCTAA
ACTAGTAGCAAGTAATTGGTATAATAGACAAATAGAAAGATCTAGTAGGACTTTGGGTTGCTCATGGGAA
TTTATTCCTGTAGATGATGGATGGGGAGAAAGGCCACTGTA

Protein Information

Protein Name

botulinum neurotoxin type A1

NCBI Protein GI

153936924

Protein Accession

YP_001386738

3D structure: PDB ID

3NF3

Protein pI

6.31

Protein Weight

142277.54

Protein Length

1296

Protein Note

identified by similarity to SP:P10845; similarity to SP:P30996; match to protein family HMM PF01742; match to protein family HMM PF07951; match to protein family HMM PF07952; match to protein family HMM PF07953

Protein Sequence

>YP_001386738.1 botulinum neurotoxin type A1 [Clostridium botulinum A str. Hall]
MPFVNKQFNYKDPVNGVDIAYIKIPNAGQMQPVKAFKIHNKIWVIPERDTFTNPEEGDLNPPPEAKQVPV
SYYDSTYLSTDNEKDNYLKGVTKLFERIYSTDLGRMLLTSIVRGIPFWGGSTIDTELKVIDTNCINVIQP
DGSYRSEELNLVIIGPSADIIQFECKSFGHEVLNLTRNGYGSTQYIRFSPDFTFGFEESLEVDTNPLLGA
GKFATDPAVTLAHELIHAGHRLYGIAINPNRVFKVNTNAYYEMSGLEVSFEELRTFGGHDAKFIDSLQEN
EFRLYYYNKFKDIASTLNKAKSIVGTTASLQYMKNVFKEKYLLSEDTSGKFSVDKLKFDKLYKMLTEIYT
EDNFVKFFKVLNRKTYLNFDKAVFKINIVPKVNYTIYDGFNLRNTNLAANFNGQNTEINNMNFTKLKNFT
GLFEFYKLLCVRGIITSKTKSLDKGYNKALNDLCIKVNNWDLFFSPSEDNFTNDLNKGEEITSDTNIEAA
EENISLDLIQQYYLTFNFDNEPENISIENLSSDIIGQLELMPNIERFPNGKKYELDKYTMFHYLRAQEFE
HGKSRIALTNSVNEALLNPSRVYTFFSSDYVKKVNKATEAAMFLGWVEQLVYDFTDETSEVSTTDKIADI
TIIIPYIGPALNIGNMLYKDDFVGALIFSGAVILLEFIPEIAIPVLGTFALVSYIANKVLTVQTIDNALS
KRNEKWDEVYKYIVTNWLAKVNTQIDLIRKKMKEALENQAEATKAIINYQYNQYTEEEKNNINFNIDDLS
SKLNESINKAMININKFLNQCSVSYLMNSMIPYGVKRLEDFDASLKDALLKYIYDNRGTLIGQVDRLKDK
VNNTLSTDIPFQLSKYVDNQRLLSTFTEYIKNIINTSILNLRYESNHLIDLSRYASKINIGSKVNFDPID
KNQIQLFNLESSKIEVILKNAIVYNSMYENFSTSFWIRIPKYFNSISLNNEYTIINCMENNSGWKVSLNY
GEIIWTLQDTQEIKQRVVFKYSQMINISDYINRWIFVTITNNRLNNSKIYINGRLIDQKPISNLGNIHAS
NNIMFKLDGCRDTHRYIWIKYFNLFDKELNEKEIKDLYDNQSNSGILKDFWGDYLQYDKPYYMLNLYDPN
KYVDVNNVGIRGYMYLKGPRGSVMTTNIYLNSSLYRGTKFIIKKYASGNKDNIVRNNDRVYINVVVKNKE
YRLATNASQAGVEKILSALEIPDVGNLSQVVVMKSKNDQGITNKCKMNLQDNNGNDIGFIGFHQFNNIAK
LVASNWYNRQIERSSRTLGCSWEFIPVDDGWGERPL

Epitope Information

IEDB Linear Epitope