VIOLIN: Vaccine Investigation and Online Information Network

LcrV from y. pestis CO92

General Information

Protegen ID

Sequence Strain (Species/Organism)

Yersinia pestis CO92

VO ID

VO_0010874

Taxonomy ID

214092

Molecule Role

Protective antigen

Molecule Role Annotation

MAb BA5 protected animals against plague, neutralized the Y. pestis type III secretion pathway and promoted opsonophagocytic clearance of bacteria in blood. LcrV residues 196-225 were necessary and sufficient for MAb BA5 binding. These results identify LcrV residues 196-225 as a linear epitope that is recognized by the murine immune system to confer plague protection (Quenee et al., 2010).

Related Vaccines(s)

Recombinant Y. pestis V antigen vaccine

References

Quenee et al., 2010: Quenee LE, Berube BJ, Segal J, Elli D, Ciletti NA, Anderson D, Schneewind O. Amino acid residues 196-225 of LcrV represent a plague protective epitope. Vaccine. 2010; 28(7); 1870-1876. [PubMed: 20005318].

Gene Information

Gene Name

LcrV from y. pestis CO92

NCBI Gene ID

1172676

Genbank Accession

AL117189

Locus Tag

YPCD1.31c

Plasmid No

pCD1

Gene Starting Position

21934

Gene Ending Position

22914

DNA Sequence

>NC_003131.1:21934-22914 Yersinia pestis CO92 plasmid pCD1, complete sequence
GTCATTTACCAGACGTGTCATCTAGCAGACGTTGCATCACTGAATCATATTTCTGAATGAAACGGTTCAG
TGCTTCAATAGCTGAATTAAAACGTGATGTAATATCAGACAGCTGAGTTGTTTTTTGGCTAACCAAGTCG
TTGAGCGGCCTGGACTTATCCGAGCAGGTGGTGGCAAAGTGAGATAATTCATTATTATCTTTATTATAAG
AGTATGAGTTTTTCAGATTACCCAACGCCCCGGTTCTTTTATTCTCACTTCCAAGAAAGTCCTTTATCGA
GACTATTTTTTTCTCGCTCCCATCCACCTGAATGGTGGTTTGAGGCATTTTCTCGAGAATTTTGTACTCT
GCGCTGGCTTTAAAAATCTCTTCATCTGTATAACCATATAAATTTTTATCCATGAGATTAATGGATTTAT
CATGGATATTTATGGTGCCACTACTAGACAGATGCTTATTAATTTCGGCTTGAATAACTGAATAAATCTT
TAATTCGGCGGTAAGCTCAGCTAATTCTTCACGCAACTTGCTACGGGCATCACCATGATGATTCATTGAA
TCAACAATCACTTTCAAAATATCATCATCGATACGATCGGCGGTTAAAGAGAAATGCATTACTGCCATGA
ACGCCCGCAATTCCCATTGTGTATTCGGCGATGATTCAAGGAACTCTTTTACTCGCTTGATGCCATTTTG
CAGTTGGTTGTCATAATGACCGCCTTTAAGAATGGCATCCTCGGGTAGAAAATAAGCTAGGATTTTCTTG
AGCAATTCGATATCATCAGTAATTACTCTATTGGCAAAAACCTCCGAATCTTTTCTGGGATCATATTTAA
TGGAAATATCTATATTTTTATCTTTGACTAACTGAACCAATTCTTCTAAAACTGAAGAACCATGACCAGT
AAGTTGTTCCACCCTAACTTTTTCTAGATCCTCAATAAAATGTTGTGGGTTTTGTTCGTAGGCTCTAATC
A

Protein Information

Protein Name

secreted effector protein

NCBI Protein GI

16082719

Protein Accession

NP_395165

3D structure: PDB ID

1R6F

Protein pI

5.66

Protein Weight

35651.56

Protein Length

326

Protein Note

V antigen; low calcium response protein V; functions in needle complex protein export; Yop secretion and targeting control protein; important for translocation pore formation; induces IL-10 production by macrophages; interacts with Toll-like receptor 2

Protein Sequence

>NP_395165.1 secreted effector protein (plasmid) [Yersinia pestis CO92]
MIRAYEQNPQHFIEDLEKVRVEQLTGHGSSVLEELVQLVKDKNIDISIKYDPRKDSEVFANRVITDDIEL
LKKILAYFLPEDAILKGGHYDNQLQNGIKRVKEFLESSPNTQWELRAFMAVMHFSLTADRIDDDILKVIV
DSMNHHGDARSKLREELAELTAELKIYSVIQAEINKHLSSSGTINIHDKSINLMDKNLYGYTDEEIFKAS
AEYKILEKMPQTTIQVDGSEKKIVSIKDFLGSENKRTGALGNLKNSYSYNKDNNELSHFATTCSDKSRPL
NDLVSQKTTQLSDITSRFNSAIEALNRFIQKYDSVMQRLLDDTSGK

Epitope Information

IEDB Linear Epitope