VIOLIN: Vaccine Investigation and Online Information Network

YapF

General Information

Protegen ID

342

Sequence Strain (Species/Organism)

Yersinia pestis CO92

VO ID

VO_0011005

Taxonomy ID

214092

Molecule Role

Protective antigen

Molecule Role Annotation

Using a mouse model, YPO0606 (yapF) partially protected mice from infection with Y. pestis at a high challenge dosage (200x LD(50)) (Li et al., 2009).

References

Li et al., 2009: Li B, Zhou L, Guo J, Wang X, Ni B, Ke Y, Zhu Z, Guo Z, Yang R. High-throughput identification of new protective antigens from a Yersinia pestis live vaccine by enzyme-linked immunospot assay. Infection and immunity. 2009; 77(10); 4356-4361. [PubMed: 19651863].

Gene Information

Gene Name

YapF

NCBI Gene ID

1173449

Genbank Accession

CP009973

Locus Tag

YPO0606

Gene Starting Position

661724

Gene Ending Position

664009

DNA Sequence

>NC_003143.1:661724-664009 Yersinia pestis CO92 chromosome, complete genome
TCTAGAACGCCCAGCCCAGATTTGCCCAGCCGGTGAAATCCTGACGATCACTATTGACCATGCCCGATAG
CTGTAGGCTGGCAGACACACCGTTATCGCTTACCAGTGCCATCCCCAGATCGAAGGTTCCTTGAGTGGTT
TTATCCTTGGTGCTTACGCCAACTACCGGCGAATTAGGGATACTCTGATCGATGGTGATCTCATCGCCAT
ACAACATTTGCTGGAATCCGACATTAAAGAACGGCTTAAGCAGTGTCTCGTTGGTCAGTGGATAGCGACC
ATCGAGACGTACTCCAGCATGGAGAGCGACTGCATGGTCGCTGTAGCTGCTATAGCGGGTGTCGATAAGA
TCATAGTTTTTGTTATTGGATGAGACAGTATAGCCATCGCGTTGCTGCCAGATATGCGTCAGCCCGACCA
TCGGTGTCATGGCATAGTTGCGATTCAACTGCCAGCTATTACCCACGGAGACATCGGTGTAGAGGCCACT
GCTATCATAGCTGCCGCTCTGGCTGCCCACGCCGTAGTAGGTATTGCTGTCACTGTAATCGGTGTCACTG
CTGAACCACGTACTGGTCGCTGCAATAAACAGACTCTCAGAGAGCTTCATTCCGCCCTGAACCCCCAGCA
TCAGCGTATTAATCTCCTCCTTACGCCCTACAGAGGTACCATTAAAATCGGTGCTGGCACTCTCATAACC
GCCGTGCCAGCCAAGCTGGAAATCGCCAAAATGTTGGTTCTGACCGAGCAATACCCCATAGCGCTGACCG
CTGTAACCGGAGGTCACTGGATCACGACGGTACTCACCAGCATAAGGTGTGATAAAGACGCAGCTATCGG
CACTCTCCAACTTCTGGTTACAACCGGCGTTACTTACCAGCTGACTATGCAGTTGCGAGGAGATGATATT
GGATGCCTGCTCCATGGAAGAACGCTGTGCCAATGCGGAGACTCCGGCATGAGAGACATCCGGTGCATAC
ACCAGTGCAGCAGATCCCAACGAACTACTGCTGCTACCGCTGTGAATCACCTTGATATCAGGCGTAATAC
CGTACAACCCCGCAATAGAACCCCCCACCGTACTCTGCTGGCCGGAGGTGGTATCGTAAGCCAGATTGTC
CAAGCTGTACTCTTTACCATAGGCGAAACCTTCACTGGCCTGCCCAGCCATCAGCACCAGATCGGCCCCT
TGCGTACCGTTACCCAGCACGATCGAACCGCCATTACTGACCGCCCACGGTGTCTCATTGCCCAAGGCGA
GTGTATAGCGTTCAATATTAACTACCGAACCGTCGGCCATCACTTCATAGGCTTGCTGTTGGCTTCCGCC
GGTGGCTGTCGCCAAAATTCTGCCGCTGGAGTTGATCGTGGCACTGCCGTTGAGCACATGAATACCATAG
GCGGATGCACCGCCCTGCGCACTCTCGCTAAATGCATCAACGATCAGTGTCCCGTTGTTGTAGATGTAGA
CCCCTTCGCCCTCGGTACTGATCGCGCTTGCGGTCGCATTGTTACCGCTGGCCGTGACTGACAACGAGCC
ATTGTTGCTCATCAGATGAGTGGGGTTAGCGGGGTTCCATGGATCGGGATCGACGCCTGGGTTGGCAGAG
AGGGTATGCAGGCCGGTTGCCCGTGCATCGCCAGCACTGGCATGAGCACTGACATCCAGACGGCCATTGT
TGACCACGCCATTACCGGCATTGGTATAGATACCGCTGGCAAAGGCGTCCTGCTCGGCCGTGGCACTCAC
CTGCATCACACCCTCGTTGCTCAACATCATGTGGTCACCGTAGTGATCCCCGGCGGCATCGGAAAAGGCC
AGGCCAGCCTGGATACCATAGACCTCTGCACTGCCGTGTAGGCTGTTGCTCGTGGCACGGATGCTACCCT
GATTACGCAGAGTGGACCAATAGGCATCCGTTGCCTGCAACCCGCTGGCAACAGCATGTTGATCCTGACT
GCTGGCACTCACGGTGATCGAGCCCTGAGCCTGATTAGTAAAGGTGCCGGAGGTGACCAGTACGCCATTC
GCCTGAGCGTCACCTTGAGTGGGTTCAACCCGCAGCGACAGTGCGCCTGCGTTGGTCGCATCCACTGCTT
GCCCTTCTGCGTTGGCGGTCAGATCTGCCAATGAAAAGTTATTGACCTCTGGTGCGGCGATCACAGTATG
ATCGCCCGTGTTATGCGGTTGCTGAGCGAAGTGGAGGGTCTGATCGGCATAGCTATTACCGCTAATCAGT
AAAGCAACAGCAACGGCGGATAAACGCCAGATCTTATGGTTATTCA

Protein Information

Protein Name

autotransporter protein

NCBI Protein GI

218927800

Protein Accession

YP_002345675

Protein pI

4.43

Protein Weight

74393.43

Protein Length

761

Protein Note

Previously sequenced, Yersinia pestis putative autotransporter protein YapF TR:CAC14225 (EMBL: AJ277629). Similar to Acidovorax avenae subsp. avenae flagellin N1141-FLA1 TR:BAB16756 (EMBL: AB040139) (492 aa) fasta scores: E(): 0.0011, 20.8% id in 476 aa

Protein Sequence

>YP_002345675.1 autotransporter protein [Yersinia pestis CO92]
MNNHKIWRLSAVAVALLISGNSYADQTLHFAQQPHNTGDHTVIAAPEVNNFSLADLTANAEGQAVDATNA
GALSLRVEPTQGDAQANGVLVTSGTFTNQAQGSITVSASSQDQHAVASGLQATDAYWSTLRNQGSIRATS
NSLHGSAEVYGIQAGLAFSDAAGDHYGDHMMLSNEGVMQVSATAEQDAFASGIYTNAGNGVVNNGRLDVS
AHASAGDARATGLHTLSANPGVDPDPWNPANPTHLMSNNGSLSVTASGNNATASAISTEGEGVYIYNNGT
LIVDAFSESAQGGASAYGIHVLNGSATINSSGRILATATGGSQQQAYEVMADGSVVNIERYTLALGNETP
WAVSNGGSIVLGNGTQGADLVLMAGQASEGFAYGKEYSLDNLAYDTTSGQQSTVGGSIAGLYGITPDIKV
IHSGSSSSSLGSAALVYAPDVSHAGVSALAQRSSMEQASNIISSQLHSQLVSNAGCNQKLESADSCVFIT
PYAGEYRRDPVTSGYSGQRYGVLLGQNQHFGDFQLGWHGGYESASTDFNGTSVGRKEEINTLMLGVQGGM
KLSESLFIAATSTWFSSDTDYSDSNTYYGVGSQSGSYDSSGLYTDVSVGNSWQLNRNYAMTPMVGLTHIW
QQRDGYTVSSNNKNYDLIDTRYSSYSDHAVALHAGVRLDGRYPLTNETLLKPFFNVGFQQMLYGDEITID
QSIPNSPVVGVSTKDKTTQGTFDLGMALVSDNGVSASLQLSGMVNSDRQDFTGWANLGWAF

Epitope Information

IEDB Linear Epitope