VIOLIN: Vaccine Investigation and Online Information Network

ireA

General Information

Protegen ID

279

Sequence Strain (Species/Organism)

Escherichia coli CFT073

VO ID

VO_0010985

Taxonomy ID

562

Other Database IDs

CDD:139605

Molecule Role

Protective antigen

Molecule Role Annotation

Immunization with IreA protects against urinary tract colinization by E.coli CFT073 and reduces colinization by E.coli 536 in the bladder of CBA/J mice (Alteri et al., 2009).

We demonstrated for IreA, antigen-specific serum IgG represents a strong correlate of protection in vaccinated mice (Mobley and Alteri, 2015).

COG

COG4771P, under P: Inorganic ion transport and metabolism

Related Vaccines(s)

E. coli IreA protein vaccine

References

Alteri et al., 2009: Alteri CJ, Hagan EC, Sivick KE, Smith SN, Mobley HL. Mucosal immunization with iron receptor antigens protects against urinary tract infection. PLoS pathogens. 2009; 5(9); e1000586. [PubMed: 19806177].

Mobley and Alteri, 2015: Mobley HL, Alteri CJ. Development of a Vaccine against Escherichia coli Urinary Tract Infections. Pathogens (Basel, Switzerland). 2015; 5(1); . [PubMed: 26729174].

Gene Information

Gene Name

ireA

NCBI Gene ID

1037614

Genbank Accession

AE014075

Locus Tag

c5174

Gene Starting Position

4935989

Gene Ending Position

4938037

DNA Sequence

>NC_004431.1:4935989-4938037 Escherichia coli CFT073, complete genome
TATGAAGAACAAATATATCATTGCTCCGGGCATTGCCGTGATGTGTTCTGCAGTTATATCATCAGGTTAT
GCCAGTTCTGATAAAAAAGAAGATACGCTTGTTGTTACTGCCTCCGGGTTCACTCAGCAGCTCAGAAATG
CCCCGGCCAGTGTCTCAGTCATTACTTCAGAACAACTGCAAAAAAAACCGGTTTCAGATCTGGTCGATGC
AGTAAAAGATGTTGAAGGGATTAGTATCACTGGTGGGAATGAAAAACCGGATATCAGTATACGTGGTCTA
AGTGGCGATTACACGCTGATTCTGGTCGATGGACGACGTCAGAGCGGTCGGGAATCCAGACCAAACGGCA
GCGGCGGTTTTGAAGCCGGATTTATCCCTCCTGTGGAAGCAATTGAACGCATTGAAGTGATCCGTGGCCC
TATGTCTTCCCTGTATGGTTCTGATGCCATCGGAGGGGTCATTAATATCATAACCAAACCAGTTAATAAC
CAAACATGGGATGGCGTACTTGGACTTGGGGGGATTATTCAGGAACATGGGAAATTTGGTAACTCAACCA
CAAATGACTTCTATCTGTCAGGCCCATTGATTAAGGATAAACTTGGTCTTCAGCTATATGGAGGAATGAA
CTATCGCAAGGAAGATAGTATCTCTCAGGGAACACCGGCAAAAGATAATAAGAATATAACGGCAACGCTC
CAGTTTACTCCGACTGAAAGCCAGAAGTTTGTTTTTGAATATGGAAAAAATAACCAGGTGCATACATTAA
CACCTGGTGAGTCTCTCGATGCCTGGACTATGCGGGGAAATCTTAAACAACCAAACAGTAAAAGAGAAAC
GCATAATTCACGTAGTCACTGGGTAGCAGCATGGAATGCCCAGGGCGAAATACTGCATCCTGAAATTGCT
GTTTATCAGGAGAAAGTTATTCGTGAGGTTAAATCAGGTAAAAAAGATAAATATAATCATTGGGATCTTA
ATTACGAGTCAAGAAAACCGGAAATAACCAACACAATCATAGATGCAAAAGTGACGGCATTTCTGCCGGA
AAATGTACTGACCATCGGAGGTCAATTTCAGCATGCAGAGCTCCGTGATGACTCAGCCACGGGTAAAAAA
ACGACAGAAACACAGTCTGTTTCAATTAAACAGAAAGCTGTTTTTATAGAAAATGAATATGCAGCAACGG
ATTCTCTCGCCCTGACTGGAGGACTGCGTCTCGATAATCATGAAATCTATGGCAGTTACTGGAATCCAAG
ATTGTACGCTGTTTATAACCTGACCGATAATCTCACACTCAAAGGGGGGATCGCAAAAGCATTTCGGGCT
CCTTCAATTCGTGAGGTGAGTCCTGGATTTGGAACACTGACGCAGGGTGGTGCCTCTATTATGTATGGAA
ACAGGGACCTGAAACCGGAGACCAGTGTAACCGAAGAGATCGGTATTATTTATAGTAATGATAGTGGTTT
TTCGGCGAGCGCGACGCTGTTTAATACTGATTTTAAAAATAAGTTGACCAGTTACGATATAGGTACAAAA
GATCCAGTCACCGGGTTAAACACTTTTATTTATGATAATGTAGGTGAGGCAAATATCAGAGGGGTGGAGC
TTGCAACTCAGATTCCTGTGTATGATAAATGGCATGTATCTGCAAACTATACATTTACTGACTCTCGTCG
AAAAAGTGATGACGAAAGTCTCAATGGCAAGTCGCTGAAAGGGGAACCTCTGGAAAGAACTCCCAGACAT
GCAGCCAATGCAAAACTGGAATGGGATTACACTCAGGATATTACATTTTATTCATCTCTGAATTATACGG
GAAAACAAATCTGGGCAGCACAAAGAAATGGTGCTAAGGTTCCCCGCGTTCGTAATGGATTCACATCTAT
GGATATTGGTCTAAATTACCAGATTCTGCCAGACACGCTGATTAATTTTGCCGTTCTTAACGTCACAGAC
AGAAAGAGCGAGGATATCGATACCATTGATGGTAACTGGCAGGTCGATGAAGGACGCCGTTATTGGGCTA
ATGTAAGAGTATCCTTCTG

Protein Information

Protein Name

IreA

NCBI Protein GI

AMR36194

Protein Accession

NP_757022

Protein pI

6.54

Protein Weight

71893.67

Protein Length

729

Protein Note

putative iron-regulated outer membrane virulence protein; Provisional

Protein Sequence

>AMR36194.1 IreA [Escherichia coli]
MKNKYIIAPGIAVMCSAVISSGYASSDKKEDTLVVTASGFTQQLRNAPASVSVITSEQLQKKPVSDLVDA
VKDVEGISITGGNEKPDISIRGLSGDYTLILVDGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGP
MSSLYGSDAIGGVINIITKPVNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLIKDKLGLQLYGGMN
YRKEDSISQGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQVHTLTPGESLDAWTMRGNLKQPNSKRET
HNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPEITNTIIDAKVTAFLPE
NVLTIGGQFQHAELRDDSATGKKTTETQSVSIKQKAVFIENEYAATDSLALTGGLRLDNHEIYGSYWNPR
LYAVYNLTDNLTLKGGIAKAFRAPSIREVSPGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYSNDSGF
SASATLFNTDFKNKLTSYDIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWHVSANYTFTDSRR
KSDDESLNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVRNGFTSM
DIGLNYLILPDTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRVSF

Epitope Information

IEDB Linear Epitope