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pTVG4 DNA vaccine plasmid |
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DNA Vaccine Plasmid ID |
70 |
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DNA Vaccine Plasmid Name |
pTVG4 DNA vaccine plasmid |
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Plasmid VO ID |
VO_0000324
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Promoter Names |
CMV |
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Antibiotics Resistant Gene |
kanamycin |
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Plasmid Description |
The plasmid pTVG4 is a derivative of the pNGVL3 vector obtained from the National Gene Vector Laboratories (courtesy, Dr. Robert Gerard). Two copies of a 36-bp CpG-rich immunostimulatory DNA sequence (ISS) containing the 5′-GTCGTT-3′ motif were added downstream of the multi-cloning site of the pNGVL3 vector to produce the immunization vector, pTVG4 (Johnson et al., 2006). |
| References |
Johnson et al., 2006: Johnson LE, Frye TP, Arnot AR, Marquette C, Couture LA, Gendron-Fitzpatrick A, McNeel DG. Safety and immunological efficacy of a prostate cancer plasmid DNA vaccine encoding prostatic acid phosphatase (PAP). Vaccine. 2006; 24(3); 293-303. [PubMed: 16115700].
pTVG4 map: [http://www.google.com/patents/US20040142890]
pTVG4 sequence: [http://www.violinet.org/dnavaxdb/docs/pTVG4_nt_seq.pdf]
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