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pNL3-gal DNA vaccine plasmid

DNA Vaccine Plasmid ID 55
DNA Vaccine Plasmid Name pNL3-gal DNA vaccine plasmid
Plasmid VO ID VO_0000297
Manufacturer Invitrogen
Plasmid Description Plasmid pNL3 was constructed by inserting the 1050 bp EcoRI/HindIII-digested DNA fragment carrying the spa40 gene into the corresponding sites of the low-expression vector pSU18 (Invitrogen) (Botteaux et al., 2010).
References
Botteaux et al., 2010: Botteaux A, Kayath CA, Page AL, Jouihri N, Sani M, Boekema E, Biskri L, Parsot C, Allaoui A. The 33 carboxyl-terminal residues of Spa40 orchestrate the multi-step assembly process of the type III secretion needle complex in Shigella flexneri. Microbiology (Reading, England). 2010; 156(Pt 9); 2807-2817. [PubMed: 20507885].