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Vaccine Detail
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A. salmonicida live-attenuated vaccine encoding AroA |
| Vaccine Information |
- Vaccine Ontology ID: VO_0011386
- Type: Live, attenuated vaccine
- Status: Research
- Antigen: A. salmonicida 644Rb aroA
- aroA
gene engineering:
- Type: Gene mutation
- Description: The aroA gene of A. salmonicida was cloned in Escherichia coli, and the nucleotide sequence was determined. The aroA gene was inactivated by inserting a fragment expressing kanamycin resistance within the coding sequence. The aroA::Kar mutation was introduced into the chromosome of virulent A. salmonicida 644Rb and 640V2 by allele replacement by using a suicide plasmid delivery system. The mutation resulted in attenuation when bacteria were injected intramuscularly into Atlantic salmon (Vaughan et al., 1993).
- Detailed Gene Information: Click Here.
- aroA
gene engineering:
- Type: Gene mutation
- Description: This aroA mutant is from Aeromonas salmonicida (Vaughan et al., 1993).
- Detailed Gene Information: Click Here.
- Immunization Route: Intraperitoneal injection (i.p.)
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| References |
Vaughan et al., 1993: Vaughan LM, Smith PR, Foster TJ. An aromatic-dependent mutant of the fish pathogen Aeromonas salmonicida is attenuated in fish and is effective as a live vaccine against the salmonid disease furunculosis. Infection and immunity. 1993; 61(5); 2172-2181. [PubMed: 8478107].
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