Eimeria maxima 5804 Coccidiosis Eimeria spp. are obligate intracellular apicomplexan protozoan parasites and are the cause of intestinal coccidiosis. Infections of the vertebrate host with these parasites result in the induction of potent protective immune responses, and protection against challenge infections is confined absolutely to homologous species, even though both T- and B-cell responses exhibit cross-species reactivity. Eimeria maxima is the most immunogenic of the seven species of Eimeria that infect the domestic chicken (Smith et al., 2002). Baboon Papio cynocephalus 9556 Bank vole Clethrionomys glareolus 447135 Bear Ursus americanus 9643 Birds Passeroidea 175121 Brown Trout Salmo trutta 8032 Buffalo Bison bison 9901 Carnivores Vulpes 9625 Cat Felis catus 9685 Catfishes Siluriformes 7995 Cattle Bos taurus 9913 Chicken Gallus gallus 9031 Chimpanzee Pan troglodytes 9598 chinchillas Chinchillidae 10150 Copper Pheasant Syrmaticus soemmerringii 9067 Deer Cervus elaphus 9860 Deer mouse Peromyscus maniculatus 10042 Dog Canis familiaris 9615 Ducks Anas 8835 Ferret Mustela putorius furo 9669 Fish Hyperotreti 117565 Gerbil Gerbillina 10045 Goat Capra hircus 9925 Gray wolf Canis lupus 9612 Guinea pig Cavia porcellus 10141 Hamster Mesocricetus auratus 10036 Horse Equus caballus 9796 Human Homo sapiens 9606 Macaque Macaca fascicularis 9541 Mongolian Gerbil Meriones unguiculatus 10047 Monkey Platyrrhini 9479 Mouse Mus musculus 10090 None None Parrot Psittacidae 9224 Pig Sus scrofa 9823 Rabbit Oryctolagus cuniculus 9986 Rainbow trout Oncorhynchus mykiss 8022 Rat Rattus 10114 Raven Corvus corax 56781 sei whale Balaenoptera borealis 9768 Sheep Ovis aries 9940 Squirrel Spermophilus richardsonii 37591 Tree shrew Tupaiidae 9393 Trouts, salmons & chars Salmoninae 504568 Turkey Meleagris gallopavo 9103 Vole Microtus ochrogaster 79684 Water buffalo Bubalus bubalis 391902 CoxAbic CoxAbic Novartis AH VO_0001113 Inactivated or "killed" vaccine Licensed Intramuscular injection (i.m.) Gametocyte antigen(s); transmission blocking through maternal antibody transfer(Wallach, 1997) Intramuscular injection (i.m.) E. maxima DNA vaccine pcDNA-Gam56 VO_0004527 DNA vaccine Research pcDNA3.1(zeo)+ [Ref2643:Xu et al., 2013] Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction Compared with control groups, the administration of pcDNA-Gam56 vaccine markedly increased the lymphocyte proliferation activity (P<0.05) at day 7 and 14 after the first immunization. The level of lymphocyte proliferation started to decrease on day 21 after the first immunization. A similar trend was seen in specific antibody levels (Xu et al., 2013). The DNA vaccine was injected intramuscularly to yellow feathered broilers of 1-week old at 3 dosages (25, 50, and 100 µg/chick). Injection was repeated once 1 week later (Xu et al., 2013). VO_0000286 Among the 3 pcDNA-Gam56 immunized groups, the median dosage group displayed the highest lymphocyte proliferation and antibody levels (P<0.05). The median dosage group had the greatest relative body weight gain (89.7%), and the greatest oocyst shedding reduction (53.7%) (Xu et al., 2013). One week after the second injection, birds were challenged orally with 5×104 sporulated oocysts of E. maxima, then weighed and killed at day 8 post challenge (Xu et al., 2013). E. maxima Gam82 protein vaccine VO_0011467 Subunit vaccine Research Intramuscular injection (i.m.) Freund's complete adjuvant (FCA) Intramuscular injection (i.m.) E. maxima 82 kDa gametocyte antigen (Gam82) Recombinant protein preparation The Gam82 coding sequence was amplified by PCR using Proof Start DNA polymerase (Qiagen, Valencia, CA). Amplicons were cloned into the pET28a (+) plasmid vector (Novagen/EMD Chemicals, Gibbstown, NJ) downstream from an NH2-terminal His6 epitope tag, plasmid clones were verified by sequence analysis, and used to transform competent Escherichia coli BL21 Star (Invitrogen). Eluted protein fractions were visualized on 10% SDS-acrylamide gels stained with Coomassie brilliant blue and on Western blots probed with horseradish peroxidase-conjugated anti-His monoclonal antibody (1:3000; Qiagen), and stored at −20 °C (Jang et al., 2010). Ross broiler At 1 wk of age, chickens (30/group) were intramuscularly immunized with 100 μl of 300 or 600 μg/ml of recombinant Gam82 in Freund's complete adjuvant (FCA). Control animals received PBS in FCA. At 1 wk post-immunization, animals were given a booster injection with 100 μl of 300 or 600 μg/ml of recombinant Gam82, 3-1E, or PBS in Freund's incomplete adjuvant Ref1077:Jang et al., 2010]. Animals vaccinated with Gam82 and challenged with E. maxima showed lower oocyst shedding and reduced intestinal pathology compared with non-vaccinated and parasite-challenged animals. Gam82 vaccination also stimulated the production of antigen-specific serum antibodies and induced greater levels of IL-2 and IL-15 mRNAs compared with non-vaccinated controls. These results demonstrate that the Gam82 recombinant protein protects against E. maxima and augments humoral and cell-mediated immunity (Jang et al., 2010). At 7 d post-secondary immunization, animals were given PBS or 2.0 × 10^4 E. maxima sporulated oocysts by oral gavage using an 18-gauge needle (Jang et al., 2010). Three days after second immunization with 60 micrograms of Gam82, there was a significant difference in IL-2 mRNA levels between the immunized and non immunized (PBS vaccinated) groups of chickens. Levels were determined in intestinal tissue (Jang et al., 2010). Three and six days after second immunization with 60 micrograms of Gam82, there was a significant difference in IL-15 mRNA levels between the immunized and non immunized (PBS vaccinated) groups of chickens. Levels were determined in intestinal tissue (Jang et al., 2010). E. maxima oocysts with Arlacel A vaccine VO_0004229 Live, attenuated vaccine Research Intramuscular injection (i.m.) Chickens were vaccinated with Arlacel A injections prior to vaccination with E. maxima oocysts (Smith et al., 1994). Intramuscular injection (i.m.) Vedette Hens were injected intramuscularly with 0.25 ml of PBS in 0.25 ml of Arlacel A in weeks 0, 2, and 6 of the study (Smith et al., 1994). None of the hens vaccinated had clinical symptoms of infection (Smith et al., 1994). At week 16 hens were infected with 20,000 oocysts of E. maxima (Smith et al., 1994). gam56 Eimeria maxima 25336657 AY129951.2 25989587 5804 ? 56 kDa gametocyte antigen 4.61 51819.73 539 tyrosine and serine rich gametocyte protein; involved in oocyst wall formation >AAN05087.1 56 kDa gametocyte antigen [Eimeria maxima] MTRLGLAAVALALAVGPSMAVPSTTPVENQVHPYSEMSTYQEGSAPGAPEDTTTTTTSSPVSDGAEQWLE SFVRAVQRQLQLQDQMMRQLMRDIQEYLSTAFNWAENQSTAYTRVTEMMDMISNRMNAAMDSSNELMTTS DTTDPETLRRATRKYMKEVRVQDVLVDALWASLRGVQTAAWMNGVTAIEKEETTPMASRAAEEFLHRMYH NLRAAGMSEEDVAKFIPRAEYNPSEQSRNMGRKGRSFYYGGYPSYYNSPYYSYSSYPSYYNYSYPSYSYS SYPSYYRYSSYPYYNYSYPSYYNYGSYPYYSYSSYPSWYWRRLRSLATATCPDCPPLTTPSMIPTPPPMM NMMNTPPPMANMMTSMMMNTPMVPPPRTLGTEAMSLGLAPIGITGAPMTGFGVPPEFGPFGAEGIGLPTD ALGSTPEMTPFDPTTPYRTLAPMDLPPIPPPVFPETPMRPPTPFGFGPAPVPPMPF Protective antigen [Ref2643:Xu et al., 2013] gam82 Eimeria maxima VO_0011062 25336658 38565038 5804 ? 82 kDa gametocyte antigen 4.8 63301.57 661 tyrosine and serine rich gametocyte precursor protein; involved in oocyst wall formation >AAO47083.2 82 kDa gametocyte antigen [Eimeria maxima] MTRAAALAGVLALAAAGSSLALPTVLDTTTGTQVEWTETPLDTTEVTMGEMGSTTSGTTPTSTGVRMMEA ETTTPSTPEAPQQQQQMPQPQPQPQQTTPVPEAVLEAIMQEMQNIFRSSLVPGWDTVGTAADAVRQIVTR VRERLTGPLMMTEMDTGLGRTGPLSTTGATGATTGPVAALRGVTNDFLREIMIQEAVLETLWAVVRDAQE RPWLVNEQEALHAATADAVQGFLGRMHDRLRATGFSEEEVMRLLPRSRNGGCTRTGGLFDQCNDAPPSRL LGKRMYSTGYYGYGYPSYYSYGYSYPAYSHYPVSYPYYGYSWGPSYYYGSGYYGKHGYKYGHYYRRLAEQ EPRPVMPPAAATAAANLRAAAAAAAEVPPPPPPAAVPPPPPAAAAGTPAMMPPPMMGVEEPVPFRSLYPS YSWSYPAYTRVSPSYSYYTPSYSSSYYYPRYNYAYNYPLYSDYSWYDYSYPLAYSSYSSYPLSYSSYSYP LSYTYPSAFYRRLEVPDLTTTTTTHHEQQQQQQQESTTTAVPTETITTPSTRNTHSSSLRRVGERYEPIT PTQRTFYNNTEGTNNPVYTPENLTEDEPQTVWETYN Protective antigen Animals vaccinated with Gam82 and challenged with E. maxima showed lower oocyst shedding and reduced intestinal pathology compared with non-vaccinated and parasite-challenged animals. Gam82 vaccination also stimulated the production of antigen-specific serum antibodies and induced greater levels of IL-2 and IL-15 mRNAs compared with non-vaccinated controls. These results demonstrate that the Gam82 recombinant protein protects against E. maxima and augments humoral and cell-mediated immunity [Ref1077:Jang et al., 2010]. Protective antigen [Ref5040:Jang et al., 2010] IL15 Gallus gallus 395258 45383037 AJ416937 NP_989902 9031 4 29463695 29495838 + interleukin 15 8.34 21070.54 187 >gi|358485508:29463695-29495838 Gallus gallus breed Red Jungle fowl, inbred line UCD001 chromosome 4, Gallus_gallus-4.0, whole genome shotgun sequence CACCAGTGCAAACTTTATCCTAGTTTCCGTACCAACTTGCTCCATAGGTTTCCGAGGCTTGTAAGTATTT TACAAACTTTAACATCCTCTTGCTTTTTTAAAAAATTTCTTTCTTGAATTAAAAGAATGAGGGAATTTAT ACTGTTTAGCTTCAATTATTTTTTTTTAGTTGCCTTTTAAGACTGTTATAAATGTAAGAGACATACTTCA AGTATATCTGACCACCTACCTGGTGTCTCTTTCACCATTTCTCCTGTAGGAGAGGCATTAGATATTATGG AAAATTGGTGCTAGTCAGTGAAAATTTGTCACTGTTTATTAACAAACTCACAAAGCAAAGGAGGAAAAAA AGACATTTCTGTGAGACTAGTGAACATAGCAAAGAGAGAGAGAGAAAAGACTGGGAGAAAAATTATTTAC CAACCTGATGTATACTCTTGAAAGAACCATAAATATATGTATGGGACTGAAAACGGCAGTCAAGTGGAAT CCTACTGAAAATGGTGTTATGGAGACAGAGTAGCTGATAGCAATGTACTATGAAAGTATTATCTGGTTAT TAAGCCAACTAAACAATTGCATTCATGAATTTGCTGTTCAAATAACACTTTCTGCAAAATGAAATCCAAA AGGAAGGAGGAAAAGGACATGTAGGGGAGGAACTTGTGAAGGACTGTGATTTTAAGACCTGCAAAATTAC CTCTGTAAAAGCCAAAAATTCTCCTAACTGTGTATGTACATAATATTTATGATTTCTTCTATATACAGCA GATATATATGTGCTGCTTTCCTTTAAAACAGTGTGATATTGCAGGAGAAATTCCTGATTATCATTACTTC ATTATGTTTTGCTGTACTTTCCAGATAGACAATAGCACAAAAGTTCCTTTGCCCATTATAAATGTGTTCA TTCCTACTTACAGTTACTAATTTATTTGAAATGTGCTAGCCAAATGGAAAGGTACCACTTAGTTATTTTC TCTTGGAATGATATATATAAACATAGATCGACTTAGTATAAACAAACAGAGGTATAGAATTACTTCCTTT GGAGTCTATTGGACTGTTCCGAACTCCAAGAATATAAGCAACTTTATAGCCTATCATAATTACTTTAGCT TTGCGGATTCAAACAATAAGGATCACCTTCGCCAAATCCTCAGAGTGTAACATTATCTATCTCACTGCCT TTAAAAGTGTTTGAACTCCCTGGGGAAAAAGAAAATTATTGACAATTCCACTCTAAGGTGTCCCAGACTT TGACTGAAAATAGAAAAGGATTGCAGAGAACAGAGACACGGGCTGATGTGGAGTTATTGGGTAGTATAAA TAATATGCTAGATTTTTTTCTGACGGATTAGATGTCCCATCTCACTTCAGCTTGCTGTTACGCGCCTTTG CATGAACATTTTCAAGGCTGTGGCCAGTAATATATTTAATACAGACTTTCTACTGTCAGGTGCACAGTTC TCACAAATACCTTTCTCTGTGTTCATCTCAGAATGTTCAGCTTTATGCAGCCAGCACAGGAAGGAGCAGA AATATGTGGGAACTGTTCCTCCGTGTTCTGTCAAAAATGGTGTAAAATAGATCATTTCCACAGGGAGAGG CTAGCACAAGGTATGACTTTCAGGAATAAGGAAAGGCTCAATAAAAGACAGCAGCCCAACCTCAACTGTT GGAATAAATTTGTTAGTCTGTGCATAAGTACATTTTTTATCCCTATTGTTTTTCTACACGACAAGGTCTT GTTGCTTGTAACTGGTACATGGTAACTGTTTTTTGGTTGCTGTCTTAATAAAAACAAATTTTATCTATGA GATCTTTTAGGACAAATTGGAATTATTTTTAGGAGCTGTTAAATTCTGAGTCTCCTAATCTTGATGAGGC AAATCTGGCTATTTTTTCTATTACAATTTTATGATGTTATTACCCATAAAGGTACACGCATTAACAAAAT TGTTCAATATCACTGTCATAGACAGATCATTACTAGTGATGATCTTTTCTGCACAGGCCATCATGACACG GTAAAACATACTGTGCCTGAAAATGGAAATCCAAGCTGTTATCACAAGTCTATAAATACTTCTTGGCTCT CAGTGTGAAGTTGTAGTGAGTTGTATACAACTGAGGTAGCAGGCAAAAATGTCACTGCTTGGGATGGCAA GCTGCCTTCTGTGTTTGTCAGTCCCTAGCCTGCCTTTTAAATTACAGACGTGACTTTCCCTGAGTTTCTG AAGTTTGAAAATTGCTCAGAAATGGAGAGGACAAGTCCCTACCATCTGTTATTTGTTATCTGCTATTTAT TACTCTGAGAAATGTATATGAAGCACTTATTTATATATAACTTATATTTTATAAGTACTTCCAAAATTTT AATTTTTTATTTTCACACAGCCCATATTTCCAGTTTCCATTCTACAGATTTTGCTAAGACAGCAGATGAC TCTGGGAATAAATGCTGACATATTTTCAAAGTTGGCACACTTCTCTCTGCAAGGACCAAAGTTGAGTACA AAGCACTGCTAGCGGATCATGGGAAAATTTGCTCAAAGGTCCCCTTTAAGCAGGAGTTCAAAGAAATGAA ATTATATAGCAAGTCCGCAGTACCTCAACACACAGATTATTCTTTCATAGAAAAAGGGGCCTCCAATTAT ATGTAACTGATCCCAGAACAAACTTACTCTTTCACCATTTCCTTGCTGAACAATGAACCTTAGTTGCTGA TTTCAAAGGTTCCTGAATAGTGAAGGACCTTCTGCTCTTCTTCAGCCAGTGCTGTTACGAAAGCTGGTGC TATGGTCCCATGCTCAGAATGAATCTGAAGGAAGAAACATTGCAAAAAGATATAATTTTCATCAAAGCTG TCTGTACTATCTCAGTGTAAGCATAGATCTCACATAGACAGGATCACCTTTTAGATAATATTCTATAATT CTCTGTGAGAGGTCTGGGCTGGGACTTCACTTTAGCTCCATCAGGATTCATGGGTAAAATAAGGCTAGTT CTTCAAGAAAATGATTCCTCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT CTCTCTTTTTTTTTTTTTTTTTGTGTGTGTGTGTTTGTGTGTGTGTGTGAAAACAAAAACAAAACAAAAC AAAAAAAACAAGGGAAGAGCAGAAGTATTTGCAAGCAATTTATTTGCCAGCAGGAGGTTTAGCATATAAT TAAACTGTTGTCCTTGCTGGTAGGTTATTACCAGCAGGTAACAAACCATCTTACGGTATTTCGTGCTATA ACAGATGAGATAGGTCTTTAATGGTAGCAAAGGGTGATCACTTAGAAATTGTTGTGACTATAGCTGCTAT AAATTGCGATCTATAGTTTAATAGTTCAACAATTAACTCTACCACTGACACTCAGAAGCCCTTTTATAAA ATCCTCTGATTATCCTCCCTGTCCCTCTGTGGAACTGTTTTTGAAAACCTGCTTTATAAAAATACCTTTA GACTATAAGGTTAGACTCTATGACAGATAAAAGTCTTATATCAAAGTTGCTAGACCAATACAGCCATGAC TAGAATTTGTTGTATGTTTGGAGTGCCTTTTTAAATATAAGAAAAAGGCCCTTTCATAAATAAAAATTCA AATGAAACAAATATACTGAATGCAAATTGTTTTTGAGTAGAGAGAGTGCCATACAGTGAAGAACTAGCCA ACAATGTCAATTACTGCACCTTTCTCTGCCTGTAATACCTGCCAGAAGGCTGTGAGCATGAAATGCTCGG TAATTTTCTTCCCTACAGTTGAAAAAACAGCTCAGGATGGTGTGGAGAAGTTTGTTGTCTATGCCGAGTC AACAATTTGAGCTCATTGGTAATGTCAGATTCTTGTCTCCCACAAAAACCATATGTGCAGATAAAAAATA TGACAATGCTGTTTTTGACGTAATGATCTTGCAAACAAATGATATTGTAGAAGATTCATTTTGTGTACAG AAATAACAACACTGACAAAATTTCACTCCACATATCCTAAATGTCATATAACTGTTAATATGCTGTTAAC TACTATTAGCATATTATCAGTTTTGTACTCAACATGATGGAAAATTGGGCACAACCTCAGCTGCCTTTCT TGATGCCACGCTGGCTAGTTAAGAATTACAGCACCTACACCAGTAGTGCTTGCTAGTACCTCTAGCAAGT GACAAAGTGTCGTCTTCCTAGAAGGGCAAATAAGAACAGGATTAGGGGAAGGTATTGCAGGAACATGGAA ATCTATGCTCCTGACATCTGAAAGCTGCAGCTGGTTACTGCGATCAAACTCCTTGCAAGAGAAATAGGTA TCTGCATCTCTCACATGGCACATGCAGGACAGACAAGTTATCAGAAGTAGACAGTCTTTGAAAGACAGGT CCTGCCTGATTGAACTTATCTCCTATGACAAGATGACTTGCTTAGTAGATAAGGCAAAGGCTATGGATGT TTTTTATCTCAAGCAGCATGCCAAGTGGCCGAGAAAGCCAATGACTCCTGACCTGCATCAGAAATACAGT GGCTAGCATGACTGGGAAAATGATTTTTCCCTTGTACATGCAAATAGTAAGACCTTACATTGCATTCAGT CTTGGGCCCCTCACTGCAAGAAGGACATTGAATTCCTTGAGGGTGTACAAAGAGCAACAAAGCTGGTGAA GGGACTAGAAAACAAAACTTACTAGGAATGGCTGAGGGAACTGAGGTTGTTTGGTCTAGAGAAGAGACTG AGAGGAGATCTCACTGGTCTCCACAAGGAGAGCTGCATCAAGGAGGGTGCCAGTCTCTTTTCTCAGGTGC TGAGTGAGACTACATAAAGAAATGGTCTCAAGTTGTGCCTGTAGAGATTTAGATTGAACATTAAGAATTT CTTTACAGAGAAGGTGCACAAGCACTGAAATGGGCTTCCCAGTGAATGGTGGAGTCTCCTATCCCTGGAA GTGTTTAAGTGTGGACATGGCACTAACAGACATGGTTTAGTGGTGGGACTCAATAGGTCAGGCTGATGAT TGGACTTGATCTTGAAGGTCTTTTCTAACCTAGATGACTAAGTGATTCTATGAATGATGTATTCCCAGCT TGAATGTTATATTAGATTGACTAAATTTTAAAACAACAGGCACCTGCATACCTGTTTAAACTGGGTGCAT GTAACATGCTTCTAAGAATGTGGGGATGGGAGTCGTAATAATCTCCTGCAGGCTCATCTACTGTAGCCTG TGGTGGAAAACAGAAAGTATGGTCTGTTTCCTTGCCTAATGTAGTTTGGTAAGGTTGTAGATGCTAGGTA GAGTTTTAATTTATGCTGATTCTTGGAAGAGTCATTGGATCAAATAGCGTTTCACATTCCTGGAGCAGAA TAAATTCTGATATAACAGTTAAAATAATTGGCTGTACTTTGGGCTGCTATGTGCAGCACAGATTACCAAT ATACAGCAGAGGGGATGAGATAGAAATTGGCACTGAGTGTCCACCGGGGGTTGCACCGGTCCCTCTTTTC AAGTCAGGCTGGGGATTCTGGAAGTTGACTTGATGTTGTGATGGCCAACACCAGACAATGGTGATGGCAA AAGCCAAAGGAATTGGTCTTTACATGTTGCCCGACTGCTTACATCTTTCTTCCATTAATTTTATTTAATT TTACATCATTTTTTGTTTAATTGTAAAGAAGTCTACAGCCTAAGTATCCTTTCTATTTTATAATATAGTA ATTAGTTACATGGGCACAAGAAGTAAGACTGTACTCTGACTAATATATATACAGTAGAAAAATTAGATAT TCTTCTTTTGCACATCCTCATATCTGATTTGCTGCACCTTCCTTGCAATGTGTTGTAAATGTAGAATAAT TAATTTTATCTTTTTTTTTTTTTTGCCTTCCATTTATAGCACTCAGCAGTACAATAACAAATTGCTTCTT GAACATGAATCAGTTCATTTTCTCAAAAGCCCTCAAGGATAATGAAATGGGAATTTTATTTTATAAGTGA AAAAAAAAAATCTGAAGAATCTAGTGATTTGGGGTCTGGTATGTGATACCTGGAGATGAATTTTTTTAAG AGACTGTTAAGCATTCTCGAGGATTGTCTCTGTCTGTATATAGCTCCCAATCTGTTCTGTTGCTGCTGTG ATAACTCAATTATTTTTTGAATTAAGATCTGTAGAGGGCACTTATTTCTTCAAACTGTAACCACCTTCAT TGGAGAAACCTCCATCTGAGCTAGCTGACTGTAAGGACTAGAAATATATGTTTGGGGAGCATGAGGCAAG TCATCCTAAAGGCAGGCACACAAAGCAACTTAAAACCAAAATTCCATGTAGGAGGGAAATCACAGAATCA TCAAGGTTGGGAAAGACCTCCAAGATCATCTAGTCCAACCATCTGAACGATTGATGCTTGTGAAAAATCT GGGTTAAGTAACTTGCCCAAATCAAATGGAGATGGTAAGATGAAGTCAAGGTTGGGATTGTTGCTCAGAG CTGCTAAACTCAACTGCTCTTGTCATTGAACTTACATTTTTCTTTCTGTTATCTTCTGCTTCCTTTTGTA TACATCTTCTTTTTTCAAGTGAAGCGGTTGATACAGTCTCTTTCACAAGATTGGCCTGTTCAGCAAACAG GTTCTGGTCAGACCTGCTTCTGGTCATTCTGGTCAGAAATCCTGTGAAAGAAAAAGGAAATTATTATACC ATACTATCATTAAAGACTTGGTAACTTTTTAAAGTTATGAATTCACAGGAAGGTAAGGAGAGTAGGATTC AGTTTCTGTTTCTGGAGAAGAATATACTTCCGTGATCATAAATCCTCCCACTTCTGTTACAACAAAACTT TCCTCCTTCCTTTCACCTCCTCTCTCTTATGTATGCCCTCCATTCAGCCTGTTACTCAAATTCCTCTGTT GTTTTGCAAACACCATCACTGATTTCTGTTCCCTGGCTTTCACCACATCATTCCTGCAGGAAGCCTTTTC CTGCAGGATACCTTAAGTTCTGCTTGCCTCTGAAGATCTGAGATGGAGTTTGTTCACTGTGAAGAGACTG CTGGAGACCAAATAATCATTGCCAAGAAATCTCTTCTGTAATTACGTGAACTAACCACTTGTGACTTTGC AAAATTTGTGCAGATTTTCTAGGCTGCCACATAGAACAAATTACTAAAAAGCTTTAGATTACTAAATTAC TAAAAGAGCTTTAATTAGATGGTTATAAACATTTTCTTTAGTTCTTTTAGTTCTTTTATTTGCTCATTTG TTTTTGGAAATGACTGAATTATGTTTGATAAGACTTTCAGTATAGGAAATTTCAGATCTAACAATTTCAT CTTGACCAAGATGAAAGTGACCAAAACAGTTTTCTTGTTTGAAACACAGCCACAGAAGGCCCTAACAAAA CTGTAGGCAATATCACCTTTACTTGAATAGAAATAGGACTGTTTCCCCAGTCTATACATTTGAAATTATC AGAGTTTAATTTCCTAAGAAAATGACATTTATCCAATCACACATCTGTGCATGTTTTTATATTTTTGCTT CCCTCCTCTATCACATTAGTTTTTAATCCAACACCTAATTTCATTGAAGAATTTATATTGGAAGAGGTAT CTAAAGATCTTTTCTCCCAGCTTCTCAACTAAAGCAGGGACATTAGTGTAGGTTGCCCCAGATTTGGCTA AGTTTTGAATACTTCTAAGGATGGAACTTCCACAACTTCTCTAATCAGCCTCCCTTGTTGTATAACTGCC CTCACTGTGACTTTTTCCCCTCATATCACTGTTTTCTCCATTTCTTTTATTGTATATCCCTCGTAACAGT TTATATCTCTCTTCTCTATAACCTCTCTATGTAGTGGAAAAAAAAACAAGATCTTCTTTGCCTTCTCTTC TCCATGTTGCACAAACCGAAGTCCCTTAGCCTGTGTGTTCAGTCTCCAAACAATTTAGTGCCAGACATTC CAGTTTTGCAGTTTCTCTTGTACAGTGGGGTTAAAACTGTACCTAGCACTTCAGATATAGCCTGTATCAT GTAGAGCACCACTTCAGAAACATCATGGATTTTTTGATCCCTTCATCTTGACTTTCCATCAGATGCTGGG TAGTTTAATCCAGTCTGCAAATGTGAGACTTTTTCCATTTGTTTAAAGACAGCTTCATTCATTTTGATTA TCAAGCATCCTTCAGCAGACCCTCACTCTCACATCCTCATGCTGATTCCTCCTTTCATCAGGACTCACAC TCCTTGTACACCAGAGTTTATTGCACTAGAGCTTTGCACCCTCTGGTCTTCTCTGCTCATCTTTCCTTGC AAGTCTGTACTGAGCAATCGCAGCACACTGGTGCTGCGATGTCCCACCACATCTTCTTAATGCTAACAAG GCCATTGTCCTGTGTTTATGTGAACTGACAATTGCTCCCACTTCTTTCACAAGGTGGCACGCATAACTAT ACAGGTACTTCAGGTGGACTCTCAACTGCTCTGTTTAAACAAGTGCAGTTTAAAAGCCTTTCTCATCACC CTGTTCCATAAATTCCCAGCCTGGAAAAGCTTAAATCAGGCTTAAATCTAAGAAACCAAGTCCATTTATG AGACAAATTAAAACCAGACATTGCAACTTCACAGGTCATCTGTACAATATGAAAAACAGTAAATAAATTA GTAGCTTTGGAGATCTATTTTCTTGCAGATTACGTTGACTGCTTTCATCAAAAATACAAATATTAATATT CAGATAAATGGTACTGTCAAAGTGTCTTCATATTTTTCTGCTGTATGTCTTCAAGGTCTTGCTGTGAATA CAAAATTCGCTGTTTTAACAAAAGAAGTTGCTTGTTTCTCCAATCCCAGCACCTGTAAGACTTTAAAGTG AATGTAGAAAAAAAGTTATAAAGGAGAAGGAAAATGTGCTAGTAAGTTTTTATCATTTCATCTATAAAAA GATAATAAAAAGGGGAATGAGCTAGTTAGCTGACATGATACCTGCTATGAAAACACTGCTTAAAAGAAGT GGCATACTGGATTGCTGCTGACCTGACCTTGTTTCTATATGGTTTGCCTCATTTATTCTCTTCTAATTTA CCTCGTAAAATAGAGGAGAATGAAAGTAAAATTGCATAGCTGGACTGACACATAAAAAGGAACTATTAAA AACAAGTGTCATGCTCTGTTGATGTAATTAGGGGAACCCTTGTAGCCCTTTAGAAACCACAGAACTATGC TGATGTTAATGACTTTCATATTAGCGAATTTAGCTGCAACTTCTGTTCTAATTTTCATCAGAAGTAATAA AAAATTATCTGATGACAATATATCTCTTCCAGAGTGACTCTCCTCCCTTATATTGCAGTGTTCAGAGATT CAGTCTAAAATGGGCAGCTGGTCTCCAGATAAAAGTAATCCTTCTGAAAAGACACATCTGGTATTACTAT GAAAGTCCCATGTAAAAGTTCAGCTCAATGGTTGGAGTGCTCTTGACAGACCATCTGAGAGCCACTTTGA GCTTTATTGCAGCATTAAATACATACTTTTTTTTCACTTCTAGGTACCGCAATGTATTTCCCGATCCAGA TTCTGTTCTTCTGTTCTGAGTGATGTTACAGTTAACAAGATGCTGGGGATGGCACAGCCAACACAAAACT CTGCCGGAGCACGGAGAAGGCCGGAGAGTCAGGTATGTGTCATTTAAACAGTCTGACATTTTATGCAATG TCTGCAAAGCTCTTAGTGTTACTTACATAGTATAACAAGAAAAACTGTTAATACAGCAAATTTAAAGCTT GGAAATATTTCGAGTATTTTAGACAAGACCGCTAGCACTCCACAATTTACTATTGTGTTCTTTTCACACT GTTTTCTATTTATTCCATTCTCGGGTCTGGATTTTTACAGACCCGTATTATTTTTTTTCTCCACAGCTAA ATTCTAGCTGACATTCTGCAACTTGTGAGTCTGTAACTCCTGACCCCCAAGTGTGTTCAATATAGGAGGA TAATTGCAAGTGTTCAGCCTTTCTAGCACTCTTCAGAGCTTCTCAAAACACTTTCTGGATGATTCAGTGT CAGTTGTATCTCTAGTAAGCAGCATGACCATGTGGCCTCCTAATAATCCACAAGGTGTTAGCACATGGAA CTCAGCATCCCCAAGGCTTGCTACAGCTCCCTGCAGAGAGGCTTGGATACCTGTATACACACTCACCTGG CCATGCCCTGCCAACTCTGTCAGCCTCCTCTGGGAGCTGGACTGCTAGGTGGCCGAAATATAGCAGCCTT CTCTACACACTGGAGGTTACCAGAAAGAAAACAGTGAGTGCTCCAGCTGTGGACACTCAATTGTGCAAAG GCTGGAGCACGTATCTCAGGCAGTCAACGGGCATAGCCCTATCTGACCCCACCTTTCCTACCACTGTGCT ATAATGTAGACAGACACATCCCCAAGTAAAGCCAATCTGCCTAAATATGCAGGTTGTGGGGGGAAAATGC CACACAGGAATTGTTTGAATGGTCTTCTTGATGATTATAGCAGTGAAGTTGCTGCTTAGGGTGATTCCTT TTACAAGAATTTTTGATTCTTTTGCTGTTTATCTCAAAGCTTCTAGATAAAACTCTTCCATTCTAATTAC ATCATAGACCTGGCCCCTAGTAGAAACAGGAAGAATACAGCTCACATGATTATGGGGAAAAATTTGGAAA TCGAAGTTCACCACTTTAGCTCAACAGGACATGAGGAGGATTAGAGCACATAATCCCAGTTTATGGGACA GGTGAAAGATACAGCATAGCTAAGTCATGCATATAGTCATGAGGTTAAAATAGTAAAAATATTTCCATGT TTATTGACTGGTATTTATTGTACCTGCAGCTGTGTCATTTCTCATTCATTTGAATCATAGTAAATTTTCC TACTGGAACAATAAATTTCATTATTAGCTTCATCTAAACCAAAGAACTTGAAAGCTCAAATTTTAGCATG CCTCTTGAAAGTCCCAGTTAAAGGGAAACTTTGGGTGATACTGTTTCTAAAGCAGCTCTGCCAATCTCAA ATATTTTTCTTTAGGGATCCAAGGGCCATCTGGTCAGGGCCTCATCAGCCAGGGCCTATCCTACTAACCC ATGGAGGGAGCAGGACAAGCCACCAAGGCAGCCAGGACAGACCAGAGTCCCAGTGACAAACTAAGTCCAC AGTGAGAAAGTCCAGAATCAGGCTAGAGTTTTGAGTAAGCAGGTCAGTAACAGGTGCAGTGATGGTGATC AAGAGGTCTACAATGGTAGCCAGAGAAGACCACAGTAACAGAGAGATTTGGGGTCAAACCAGGTAGTCCA TCCACTGGCCAGGGTCACATCAACAGTACAAAAAGCCAGGCACCGGCATGAATCCAAAGAGTCAGCACCC AGGGCCCCAGTGCTTACAACCTTTGCTAGAGTCATAGCAATTTGCCAACACGGTATATTTTTCATAAGCT GAAAAAGTCACTTGTTCTAAGGCTTGATTCTTGCATTTATCTAATTTCTTACAATTCTGCTATTTACATT CTCTTGGGAGACTGTGGTAAAAGACAAACAATCGTGCTCAGGTGATCATAGTACCTTTCTCCTGTTGGAT AGATTCAGGAGTACTGTAAGCTAGAATGATGGCACTAAACCTTTTAAAAACTCATCTTGTTACAGTGCTC TCAGTTCTAAATCTCCTACCTTCTGGTCTACACTTCTTATCTATTTTCTTATCATATTCCAACTCTTCAC ACGAGACTGTAACTTCCTGAGCCACTGACTACATGTTCAGTCTCCTGCCCACTCTCTCCTACAAAAGTGC AGTTCCTAAAACTTTGCAGATGGTGCTAAAATACCATATTATACATGACAGTGACATAACAGGTCAGAAG CCAGTTACGTGCAGAGGCAGGTTAGTTTCTCTGCAGTGACTGCAGGTGACACATGAGTCGAGTCAAGGTT ATTAGCTACTCTAGGCCTGTAAAAAATTTCAAATTAATCATAAGTAAATGTTTTTTAAATTGATGCAGAT CATTTCAAATACAAATGCAATCAGTGTTATTTCCCCCACTGCTCTTAAAGAATATACAATGCTGTACTAA GTCTGTCTGGGATAGAGTTAACTTTCTCCACAGCAGCCCACAGAAGCTGTGCTCTGCACCTGCAGTTATA ACAGCCTTGATATCACACTGATGTTTTAGGCTTCGCTGGCATCCTACCAAGGCTGGATCTTTATGCCCTG TGCCCAAAGGCCAGTAGGCTGGATGTGCAAGATGTGCAGAGCAGGCACAACCAAGGCAGCTGGCCTAAGC TGGCTAAAGGGATATTCCATAGCATATAACAGTACACTTAGCTATAAAAGTTGGGGAAGGAGAAGAATTA TGAAAGTGTTGGTATGCCTAAGCAATCGCTAAACATATTGAGGTCCTGCTCCTGGAGATGTAGCTCAATA TCACTTGCTGATGGGAAATAAAGAATAATTATTTTTTCCCTTTGCTTCTGCATGGCATTTGTTTTGGTTT TGGTTTTGTTTTATTATTACATTTTTTCCATTTCCATTTAATTAAACTGTATCCCCACCTGCAAGCCTGT CTGGTTTTCACTGTTTTTACTCCCCTGTCCTTCTGTGGAGGGAAAGTGAAGAGCAGCATGGTAAAACTTA GCTGCCCATTGATTGTTAAAACGCTACAAAAGCCATTCATAGTTCATGTGGATATAGGACACTGTGATTA CCACAGTAATCAAGCTTGTTTTGCCCTTTGGACACAATAATGTTGATTCTGTTGTAACTTATCAACTTCA TCTCCTTCATTCTTCAAGATAACTTAAGAGCTTTCATGTATTCTGGGAATGATTCTATTTGGTTCCAAAG TCAGTGAACATTTTCAAATATAACTGTACCATTGCTCTCTTTTAAAGGTATATGGAAGTTCATTTTTATA GCATTTACGCCATTACTTCATCATCACTGAAATTAATCATTTGATGATATTGTTCATATTTATACAAACT GATGTAGCCATGTAGGTATAAACAAAGGAAATGATCTTTCCTGATATAATTTGCTATCGAAAAATGGATG TAACATATTTCATTAACATGTATGTTTCAGTTGTTAAAATTAAAAGTGCAGATGAGTTAAAATAGTAAAT CACAGGGTAACCATCTACTATAAGAAAAAAAGATAAAGGGATTGCACCCCTGGAAGATATAATAGTAGTA CTTTTATATAAGGATTTTTTTTGCTTTAGTTCTTCAAATGATCATTAAAGATTTAAACTTGGTGATATTT CTGTGGCTGAGCTGTCTTCTCACTTAAAATACTAGCTTAGAAATTACTGCACAAAATGTAAGAGTATTCT GTTGTTAGAAGTTTCAAAACAGTCTCTAGAGAACAGCTCAGAGAGGGATCACTCACTGAAGAACAGTCTG TTTGTTACAACAACACTCGAAAGAAATAAAGGAACCAAAGTTAACAATCTGATTTCCAGAAATCAGACCA AGTTTCCATTATCAAAATAACTTTGAGCAACTAAATCCATCTATTGGCTACTTTACTATGAGTTATGCTA GAAGAAAAGGTGCTCTTTAAAAAAGAAGTATTGAATAGCTGGTCTTTGGGAAGTTGACAGGGTTGACAGG CAATGTTATTAACCTACAGGGTCACAAGTAATTACTTACCCTGATTGTGTGTAAGGGTGCTTCTGCATGT AACTGGACTGTCTTCTGTCCACTGTGGCCTGTAGTTTGTGTGCTTCTGTTTGGAACTGTTGAAGACAGTG ACTGTTAAGGAGATTTCTGAGAAAGACTGTAGTCCTAAAATTAAGTGTATTTCATCTGGTGTTGCGCTCC ACTGATTCTGACTAGCAGCTACTAGCACAAGCAGAATTTTCTGATCCCTTTTAAGTTTCTATGATAGTTT ACCATACAATTTGAAATTACACGTTTATATTAAATCACGTTAGCTGATTCACTATTCCTACTGTAAGGTT CTCTGGCTGAATTTGAATTTTGAAATTGTTTAGACATTTCACCAAAATGTACTGTCAGGCATGTTTGCAG AGCTGAACATGCATGTAACTTATTTCTGAATTGTAATTACACCAAGATTTACTGTTAAAAAGATCACTGA AATGTCAACATTACATAAAATCAATTAACCCACTTATGGAAACTAAATATATCAGCCCCAATCTCATTAT TTTTTCTTTTCTATGAAATATGTTACCATACCTGTTTCTTATTCGGTTGAATCTATTTATCTACTGAAGA ACTCAGTGCATGCAACCCTTGACTTCTGCTGTTCAGGATACACAGTTCTGTGAAGTCCATGTCAATACCA TTGAGAGTGCATACTTTTCTGTTTCCACCACTGCCTCCACTTTTGCTCTTCATCCTCAATACATAGAGCA CCATCTTTTGTTAACACAATCAACTTTAGGTCTTTAACTACAAAACAAATGAAATGCGTTTTTATTTAGC TAATTCGTGCTCGTAGTTTAATCATGTTTCATATGCAGCATTTTGTTACATCTTTGTAATTATCTACCCA TTTCCTCTACTGTACTACAAGACTACCAAGGTTGGTTAGGAGAGGAAAGCCTTCAAAAGAGCTCCAGGGA TATTCACTCCCTTGTTTCCCCTCAGAGTTATAAGACAAATACTAACCATGTAATTATTCAGTGCAAGAAA TAAAATTTGTCAAAGTTCAAAAAAGAAAGAAATTAGAAGTGAGCATGGAAGTTAGTTTGCAACATCAGTG AGCATTTCTGACTTACCACACGCTTTTAATTATATCCTCTTGGGAAGTGACTTAGTTAGGCTATAACAGG GTGGACATGCTATGTGACAAACTTCCAAGAAAACCTAGACAGACCCAGGAGAGTGTTGTTCCATTTCATT TCTGAACAAGTTGCCTGTTGGAGATACATGTCTAAGTTGCAGCTTTCCTCCAGTGTTAGGGTAGACTGCA GTCTTGACTTGTTTTACCTTTACTCCAGGATGTCCAGCCTTCTCCCAACTCAATAGCCTAAGCCTGAATC ATGTCTTTTTCTACAAAGGTGAATGAAGATGTGCTCAATCTACAGCCTCTCCAGATCATGAAAGGAACAA AGGGAAAAATCATAGAACAAACATTCTAAAGCAGATGAAAGTACAACTTTTATCACAATTTCCAAGTAGA AATTGATGTAATGCTGTTATTTTCGGTTTTCTAGATCCTGAGTAAGGGTAAAAAAATCTCTTCCACTGAG GAGCATTTCCAATTTCAGAACTGACAAATGTATATAGAAAGATTAATACTCCCTTTCTTACTTGCTCAGT TCACCACCCATAAAAAGAAGAAAGGAGCCTATATGAAAAAAAGTGACATGCATAGGACATCGTCAGAAGA CAGTACTTAGAAACATAAGAGCATGCCGAAAAATCAGCTGGAACAACTGCAGAAATAGATGGTTCTCTTC AAGACAATTTCAGTTAAAACAGCCTAGAAAAGTTTTCCAGACTAAAATTAATGACACTTGCTGACAGCAG CTGTGCATATTTCTTCACTCAGGCCACAAGACGATGAATCATTGGCAACCCCACCTCTTTTTACTCTCGT CTTTCCATACACATGCCAAGGACAGCAGAGCAAACTGCTGTCCTTACAAGTAGCATAGACTTACTAAAAC CTGTGGCCTCAGTACTTGCAGGCAGGCTCACTATATTGGCGCTGCTGCATTTGGTGAAGTTAGGACCAAA TTCATTGCAAGTGAAACAAGCACGGCTTTGGAAAAACTGCTGAAGCTTCCTGCTATTTTTTTCCATTCAT TTTGAACCCGTGTGCCTGGCTTTTCTCACTTATGTTTAGCTTCTGCAGACCCAAAGAGGACAAGCAAAAG CAGCTAGGAAAGTCAGAAAAATCTACATCTGATATATCACATCAGAAATGGTTACAAGGATCATTGTTTT TTTTTTTTTTTTTTTTCACCACAAAACCCTCAAAATCATTAATATCCTGCTGAGATTCTGATATGCAGTA TACTGATATTGAATTAAATTGTTGAAGAAAATTTACCCAGACCCAAAACAAGGTAGAATACAGCTCAATA GATCTTATAGCTCTATAACACCTACAGATACATTTAGGTAAATTTAAGATTTTACCGAATCAGTCCAATG TTCATTTTCTGCAGAAGTCGAAACATAAGTTTATCACAATGTACTCCTTTGTTCAATTTTTATAGTAAAA TTTGAAGAAAATGATGTGAAAATATCTCCCTGATCTAGGGAGAAATTTTTACTGAAAACTTTATCTCAGA AACACCTCATCCTTTTCCAGTGTAAAATTTTTCAAGATGTGGTGAAGCATTTAGGGTCTAGTACTCTTGA ATGATGAGACAGACAGAGGAAGACTCTGGCCTAAGAGCGTTGTTTTATGAGGGGGTCAGGCTTATTTCTC TCAACAGAAACATAGTGTATACATACACAGTGGGTGAAGAGTGATGTACGTGCTGGAGCTGACAAAGATG GAGCTTTGTTATGGTTATCTGTTTAGCTGACCAGAACACAAAGTGTAGCTATCCTTTCTAACATTCAAAT TTAACTTCAGCTTTGAAAAAGCAATCTACAGCAGACAGAAGTCCTGAGGATATAAACACCAGACAGTTTT ACTCATTTTCTTACCCCCTTTCTTCTACTATTCTGTATTCAGAAAACACACGACTTGCTTATTGCGCTCA AAAAACTCTATGCTAGTGACGATTGTTATCTCTGCTGGTGTTATCTAACAGTAACTGAATGCATTATAAA AATATAGTGATACAAAAGAAACATCTAGAGCATGTACATTTACTGATACAGTTATTCTTCAAGTAGCTAT TCAACTTTTTTTTCCATGGGAATATTTGCAAAACTTTTCCAGTGGTCTACAACAATCTACTTTTAAAGCA GTTCCTTCTTTCTTTCTTTTTTTCAAGCTTTTCTATTACAGGACAAAAAATCTCACTCATCTCCTGCCTT TGTGTAACTGAAAACACCTCTCAGATTCATGAGATTTTATGGAAGCCTGTAGATTATTTTGCATAATTTA AATGGCAAATGAATATTTCAGCTCTCATTTTAAACTGTGTGTGATTACAAGACAAATAGGTCACAGTGAG AACGAAGTGGTTTTCTAGTAGACTTCATCCAGTTAAAAACAAATGACTCGGCAATAATGCAAATTTATGC ACTGTGCATACGTGACGATTTTCAACAAGAAACTGAGTGACATACTGCACTGAATGTTGACTAATGAATA ACTGTTGAGGAGGTCAACTCAAGTTCTGCACTACACTTAGTAAAAGTGAGTTTTCAACACTAACATGACA CAACAAGATGGAGGACTGGTAGCTATCCTATGTTCTTTTTAGGATCTTGTGTTGGGGACCTAAAGGCCCC TGAATGAAGAATGATCCTGGGATGAAACAAGTATTCTGACAGTTTTAAGGGAAGTGGCTACAGCCACAAA TGTTTTGTTTTGTAGAGAGAACTGACCTTGGTAGTTTATCCTGTTTCTGCAAAGACTTGCTTTTCAAGCA GGTGTTCCACTTGGGCTCTGTATCGTGCATGGTATGGCGTGCAAGTGGAAAAAAACCCTGCAGACACTGG GGAGAAAAAAAGGAGGAGAGAGTCTGCAGTGGGTGAGAGAGGAAGAAGTGCATCTTGAAGCAGGGGATGC CCTGCTGCCTTCACCTATCAAATCCACTGCCAGAATGTTCCTCCTCTCCCCTTTTCAGCAATTTGCTGTT TCTCAAGGGTCTCTAAGTAATAGTGCACTGGCTTAGGGAGTTTCTGTTTGCCACTTATTGGGATTATCTA CAATGAATGCCTGCGTGTATATGAGTAAGCTGAATAAAACTCATAACCCTCTGTGCTGCTCTGTTTGTGC ATAGTTTGCTTGCCTCTGGTCCTGAGGCATCCTGACTCTCAGATCCAACTGTACCTTGAAACAGATTTGA AGGCTTTTACCAGAAGCTACACTAACAGTTCAGTGCTTATACTATACCAAAAGTATGGTAGCATCCTAGA ACTAAGACAGAATGATTTATCTCAAAGCAATTTATGTCACCCATTTTATCCTGACTTAAATATCGAGTTA CAACAAAAACTTTCATTTAAATCAGGGTTCTTAATTTTTATTTCTATTTACATACCTCAGTTACTTTCTT AAGAAAAGGTCCACACTATCACTAAAATAAATTTTCAAATCAAAGAAATCTCCATATTAAACATACTAAG AACATATATTTCTTAAGCAGTTCTGTAAGTTATACACTCAGAATCTTAACTGTTATCCTAGAAGCTTATG AATGATGATTTTTTTTGTTTGTTGTTTTGTTTCTTATTTTGCTTAAAACTTCTGTCAGTATCTGTTCTGA AGAAACGAGTACATAAATAACACACTTTAAAACAATTTGAATAATGTCTTGCATGAGTATCAAAAGTAAT TCTGAAACAGAAGAAAAAAATACCACTCTCACAGTAGTAGATTATTCTTTTTGCTCAGTATCCCTCAGAG TTTCTGAAAGACATGCAAGTACATTTAGAAAAATCAGCCTACCTATTTGAGTAGGATAAAAACCTCAATC TTACTGAATTCAATGGGATATAGATATTATCACCGCTATAGCTGACCATAACTTCAGGTCTATGGGATGT GACTTTTCTCTGGATCAGGGAGAATTCTGCAGGTACTTGTCTTGGTGACCAAAATAAGTACAGCACATTT TCCCAGGTATGTAGAATTATGCCATTTTTTACTCACAGTTTACAACTAAGAGAGAAGAAACTTTGTTTTC TCCTTTCCTAATAGTTTCTTCAGCTTTCACATGGCTATTTCAAATGATGAAAATGTTCTCACAACACTTT ATGAACTGAAGCAAAAATAAATTAGACTGAAACCACAAAGAAAAAGAATATTTGTTTTTGGTTAGAGTAA ATATGAGCATTTGTTGCACTGTAATGGCTGAAAATAATATGAATGTTTACTCAAAGTAGTGACATTATGA CTTTCAGAAAGCTTAAGCTGCCCTTAAAAAAAAAAGATATGTTCCCTTAGATTATACATGCTTATATAAA GCAGTACTCTTCAGAAAACTACAATATGAAGAAGCTTAATTAAAGTAACATTTTACATTCTTAAATTACT TAAAATCTTCTATTATGTTTAGAACTTAACACTGCCTTACAAAATTTGAAAAGTAAATTAAAAAATATTT AAGGATAATGAACTGCTAGTTAAAATTCTAAAAATCCATTACAAAGAAAATATTTATCTATACTGATAAA CAACAGGGAAGAAGTGTTCGAGAAGTTATAAAGATGAAAATACTAGCCTTAAATCTGATACTTAAATCTG CATACTTTGTTGGAGATTAAAGAGTCTATCGGGCAAATATAAGTACACTCTTCTCTTGGTATTCACAACT GGCTGTAGCTGGAGAAAGTAGGTCTAGAAGCCCTCTCTACAAATCCAGAGCCAGCTTTGCTTTTCCTAAA TCCTGTGCTTCTCTAATTCTTATCATGTTCTTATCATACTTATCTAATCCTTATCATCTAATTCTTATCC CTACTTTGTAAGATAAGTACTGCCTGGACATTAGGAGGGACTAATGAAGAGTACTGTCTGTGCCACGCTT CTTAAAACAGGATTTCCTGCAGCCCTCTGGGTGTACAGAATCTGTACAAGGATCTTTAATACAGCACAGA CCCATGTTTTCAATTAGTTGACTCCTGCACGCCTCCTACTCTAATGCAAAACTGTTACTGGCTGTACAGA CAGCCAATAACAAAGAACAGTAAGACCAGTCAGGCTCTGAAAGCAAAAGATAGGAAATCCAGTGGAGGGA AAAATACTGGAGATAGCAACTTGCAGGCACAGTTTCTAAGTGTAAAATAAGTAACAGTGTTTAATCTGAC CTAATCGATTTGCAAGAAAGATTGTGAAATGGCACGTAGCAAGAGTAGAAGGAATGGGCAGTTTGCTTTA GAAAAATCTAACAGACTTTGGAAAAACACAAGAAAGGAAGTTCTCAACCATTCTTTCTTCATAAGAATAA TAAACCAGATGTTCTGCCATTTGTAAACAGCTATTAACTTCACATCCTAGATCTTCATGATATTAATTTA TCTGCTCATACAAGGGATGTTTATAAGATATTTACTGTTTCAGTTTTTATAATAGTATGCATACATATTA AGAGATATGAGTGAATTCATTTGAGTGATTTTCTTCAAAAATGAAACCTAGTTAGTTGTTCTTACAAAGA CAACAAAGTACATTAGCCTGCGACATCATGCTTCTCTTCAGGACAAATAAATATCGTTCTTTGAAGGTAA GCATGTAGTAAGCATGCAGATCAAACACTGTATTAGCACCTGCTTAGAATGGCATAGAACTCATTTCTAT CTTTACTATCCAAAAGTAATGGAAAATAGCAGTTTGGAAAGAAGACTATGGGAAAATATTTTCTTCAACT TCTCCTCTTATCTCCTCCCACAAGGCAGATATAGCACATTTACTAGACAGTTACCTATGGCTGAATTGGC CATTTATTTCAGTGGAAACTGCTGAAGGCAGTTCATTTTTAAAGTAAGACAAATTATTACTTTTTGATTG ATGTAGATGTGAAAACATGCAAGAAATATTTCAAATTTTCAAGCAGTATTGATTTGTTTGGCAGACTGCA CTATTTTCTAAAATGTTTATTTTACTTCTCAAGAGTAATGATGGCAGTAAGCTGTTTGGTGTGGTAACAT GCCTGAGGGACAGGATGCCATCTAGAGAGACCTAGACAGACGCACGCAGTGAGCCCAGGTGAACCCCACT TGATTCAACAAATCCAAGTGCAAGATTTTGCACCTGGTTCATGGTAACCCCCACTATCCATACAAGCTGG AGGATGAAATGATAGAGCACAGCCCTGTTGAAAAGGACTTTTGGGGTACTGTTGAATGGCAAGCTAGACA CGAGCCAGAAATGTGCTCTCATAGCCCAGAAAACCAACTGTATCCTGGGCTGCACCAAAAGAAGCGTGGC CAGCAGGTCAATATATTTTGTTATTTCTATGATTCCTGATACTGATTTATTCTGCTTACTGATCTGTACT TCTCAGCAAAATATTTTGTCAAATTCTCAGCAGCTCTGGAAATGGAAATAATTTTACTGGTTTCAATAGA TAATGGACACACATTAAAATTTCATTCTGCTCCTTAAAAATTAAAAGTGTAGGAAAATAGTAAAATATTT CCAGCTTCCAGATCCTTCTTGCTTATCTCGAGAATCCAGTAAATTGTAATAATTGGTATAAGATAGGGTC ATAGGTTATAACTTTTTTTTTTTTTAATTTAAAAATGTCTCAAAATACTGCCTCACTTCACACTTCTGAT ACTGCAATTGTTACATTTAATCTATCTATTCCAGGTATATTGCAGATCTTCATTAATATGTGATATAATT CGCTGTCCCAGTGCTAATACTATGCTAAATCTGAGATACTGTCTAGCAGCTGCTCCTGTGGTAACTATTT TACAAACTTAGGGTTTTCATCTTCTCCCCATCACTATAACAGACCTTACACTTTTACTTTTAAAGTTCCT TTCTTTGACACTGTTTTTGATTTTAAAAACCTACCCCCTAATGCACAAAATCTGTAATTCTATTTTAGTT CCCTGTTGAATATTTGTTGTCATGGAATGAAAATGTAACAAAAATATTTACAGGGACTGACTAGAAGCAT ATATAGATCAGTACATATTTTGCTATACAAACTTCAGCACTGACTAGATATGCTGGTCCTTTTGCTATTC TTGTTGTTTTGTTCATGTCCTTTACAATCTCTTGTGATTCAAGAGCTTACTTATACAGGAGAGTATGTTC TGAAAAACATTTTCACTGTTTTCTGCATATTGCATCCTGAAGGGGTCTTGAGTTACACTGAGAAATTAGT GTAAGTAGATTTAAAAGGAAAGCTCTTGATGTAAATAGTAATATTCAACTAGAAAAATCTTGCCCTAAAG ACTTCTTTTGGGTTTCACTGCTGCTGTGACTGCAAGGTTCTTTAGAAAGGTATAAAATCATTCTCCGCAC CAAGGACTAACTTCTTCTTAGGCCAACAGCTAAGTATAGGATCCATCTTCATAAATGATACAAGCCCAGG CTTCAAGCATGTTTTGCAAAAGAAAGGTCAAAGGAAAGATTTAACTTCTTTCATTTCAAATTCTAATGTT TAAGATATTGAATGGAATAATAATGATTCTGTGACCTTTTGGCAATGTTTTCAAGCAGGCATTGCAAACT TTACTTTCTTGTTTCTTCACCTGGCATACAGGAGAAGGAAATCTTACTGGATGACTATCAAAAGCAAATG AACAAGTATTCCTGAAAACCAAGAACATATCAGTGCATAAGTGGCAGCTAAGCAACCACACGGTTGCTTG CTTACCATTCCCACAGTCAGATGTACAGAGGAGAAAACTGAAAGGACAGAAGTGAGGAAAACTCATAAGC TGAGATAAAAACAGCCTAATAAATGAAAGTGAAGAATAACAGAAAGCCAAAACACAAGTGATGCAGAAGT AATTACTTGTCACCTCCCACCAGTGGATCAATGTCCAGCCAGTTTCCAAGCAACGGCTGCTCTTTAAAAA ATTCACCCTCTTATCCCCACCTGTGCATTTACCTACAGATTTTAGAACTTGTGATATTATATGGAATTGA ATATGCCTTTGGGACTGCTGTCCCAGCTATGTCCCCTCTCAGCCTCTTGAAAACCCCTAGTTTATTTGCT GGCAGGGAGGGAGAGTGAGAAACAGAGAAGGCCTTAACAGTGTAAAAAATTGTTCAGCAATGGCTAAAAT ATTGGTGTATAATCAGCATTATTTTGGTCACAAATTTACAACTCAGCGCCGTTCAAGTAACTATGAAAAC ACTTAAATCCATCCCAAGCAGACTCAGTACAATAGTTAAGGTATATGGAATGTGAGAGGAGAATACTGCT GTCAATCTATTTATATCTCTGAGAGATCCCTGTGAAGGAAAGAGCTGCTGTAGACTGAGGATCAGTATTG AAATTAAATGTTCAAATAAAGAACTTGTAGGTTCAGGATCTAAATTCTCTAAGAATAAAAATACTCACTT CTTCCACAAAAGCAACATTGTGAGTTCCATAGTTGTACAAATATGATAAAAGCTAATGTTAGTATCTTTT CTGTGCTGGTATTAGGTAACAAGAACCTGTATTCACTTGTAGTTGCCAAAGGTAATTGACAAAATGTATT CAATTCTCAAGAAGATAAAATCACAGAAGGAGCAGGAACTTGTATTTTTCCATCTTGAGCTTTTCTCCTG AAAAACACCTGGGTACACCCACTTCAGATCACTGAATGTGAAGATCACCTGGAAAATCCCCATAGGGCTA ATATAAAGAAATCTCATTTGGGAGGAAAGTAGGCAAGGAGAAAGGACAGCATTATCTTGTACTACAGTGA TACTGCTTCTGTGCTCAGATTCTGAAGGGGGTTCTGCTTTTGACAGAGACCTGGCTGAGAGTGCAAATGT TTAGGTCCCAGAAGCCTGATATTAATATGGTAGAGGATGTTTGACTTAGCAGGAGAATCTTACTTCAGCA GGGTAAAAAAATGTCTTCCCTTCCTGCACATTTTCTCAATTGAATGATAGAAAATTTTATAGTAATAAAC TATGGACACAAACAATTAAGAATACTGGACCACTGTAGATAAAACTAACTTACACAGCAACTCAGTTTTT CTAATTATAATTATCACATTTCAAGGATTCACTGAGAAATTTACAAACAGCACTTAATAAAGGAATTCAC ATATACGGTAGGTAAGTGAAGTCATATAGTTTTAGCTTTCCGTAATTTCCTTTGCATGTAATTTAAATAT TGTATAGAACTATGAATATTTTCTTTTGACTATGTTTTTGTTTTGTTTGTTTTCTTTTGTTTTGGTGACA GCCAAAATGGCAAAATCAGCAGCTGCTTCTGTCTTATTCTGTCTTGCAAACTTTTGTCATCTGCTTAAGT TTCTTTCTTTAAAAGAGAGAATAGTTCTACTGTGGCTTACATTTTACTCAATGATAAGTGCTTCATTGCA ACTTGGTGATGAAAAAATTAATTTATTTTTTCAAAAGTGACAGACCATTTCTGTCTAATATAACTCTCAG TTGCATCTTACATAAAGAAATAAATAAAAAATATTCACTTACAAAGTCTTTAATCCCTACTCTGGTGTTG CAGGTTATCAGGTTATAACCTACAGGGGGGGGGGGGGGAATTATTGGAATATAAACTGCTTCATTTTCTT TTCAAAGTCCAAAGTTCAACTGTGCATAAAACAAGGGCAAGTATCAAAGGCATAATAAAGTTGATTAATG ATTAAACAGAGTAAGAAATGAAAACCTAATGCTAACATAGGGAAGGTTTTAAATAAACAAAGTGTGTTCA AAGCAAAAGAGTAGTTTGTCAAACTAAGTAAGTGAGATAGCATTCTTAAGTTGCTGAATAGTAGTTCACA TGAAATATTTAAGACATACAGGTAGAAAATTGCCCAGAATTGTAGAAATGATGTCTTAGGTGATCTACTG GACCTTAAGAACCTCTTTCTTTATAAACAAATGAATTTCCCTAAATTTTACAATTAATAAAAGGTTTCTC ACCTAAAAACTAAGTTTATTTTTCAAGTAAGGAACAATTATTCCCTTACTATTTGATGGTTGCCTACTGT TGAAGAACAGCAAAGATTAAGACAACTACATTAATCATTAAGAACACAGCGTTCCGCTGTCATCACAGTG AGCCCTAATGTATTAATTATGAACCAAAAACTGAAAAATGCTTCAAGATCCAATATAATTTTTGGTCTGA CATCTTCATTAACAGCCTCCATTGACAAAAACTAGCTTGTAAAATGATCAGAACGTTTACCTATTGCAAA ATGTTTATGATACATTTTAACTAATTTTGTTAAAATGTTTTTAAACACAAGAATGTTTTTTTTCAGCTGT AGACAAGAAATAAGATTTAAATTTCTTAGTACATATCTAGAAATCTTCTAGAAGATTATTTCTGAATTTC ATTTAAATTAATACATTAAAAAATATATTTAAAAAAACAGCAAAATAAGATATTGTTAAGAACTGCCAAA TCAAGTCAAAATAAATAGAATACACATTAAAAAAAAAAAAAAGAGTTGCTTTGCTTTGTTATTTTGTTAT TTTTTACAGAAAACAACAAATATCAGATCAAACAATGTGGAAAGGTTGTGGCCTTTCTATTGTATTCTAA CAAAATTACTCAAGTGTTTTGACAACAGCTAAGTTGTACATATTTCATTATGAGTGCTGTTTAAAGGTGT ATTTATTGTATTAGTACTTAACATTTTGGTTTGAATAGTCATATATTTTGGATTTCCATTTGTTATGGAA ATAGTACAGGTTCAGCTATGTTTTTAGATATACTTTGGTTATAGGTAACTGAAATAGTTCCAAAGATGGA TGAGATATTATTGATCTGGCCATAATTTCATTAGGTATGAAACTGAATTTACCTTTCACTTACGTACTTC TTACATAACAGATTTTGGACACAAAAGTAATACAAGAATCTGGCACCATTTTTTGTTATTGAGTGTGGAA ATGTATGTGATGGTGAGGTTACTATATTATCATCTCAGCAAAATCTTTTGGTTTTGGAAGCAAGACAATT GAAATATATTGAGAGTATTTTCTGTTTAAAACAGTAATAAATACTAGTTACAAGTACTTTTCAGAGCTCC CCTGAGGCACACACTAGTATTGCTCTCCTAGCTTTGCCAGTAGATGCTTTAATCACAGTTAATCAGCTTG CAGCAAGTTAGCAGCAACCTTACCAATAAAGTATGGATAGACCTTTCACTCTAATTTTTTTTGCTACCAC ACGGTGCTATCTTTTTTTTCTATTATGTTCTCTGAACAATTTTTAAACATGCACTTGTAAATTAGTGTGG ATATAAACCACAATGAAAGAAACACAGTTTGATAAGAAATTAAACACTGGACCAAAATCGCAGTTGCAGA TAATTAACCTACCTCTGTGAAGAAGCCGTATATGAGCTCAAATTAGTTTGATTATCAGAAATTATCACAA ATCAAAAGTTTGTGATTCTGGAACAAAAGTTCCCAGGTTGTGCACAATAGAACAGTTAGTGTACAGCTCA TACCTTCTCTTTTCCAGTTGTCAAACTCCAATGAAAGAATTGACAGACTTGAAAGGTCGGCCCGGGTGAA CCTAATGAGGTTCAACACGGCAAAGTGCAGGGTTTTGCACTTGGGTTGGAGGAACCCCAGGCATCTCTAC AGACTGGAAGGAGCAGTCCTTGAGAGCAGCTCTGCAAAAAAGGACCTGGGGGTCCTGATGGATGACAAAC TTAACATGAGCCAGCAGTGTGCTCTTGCAGCTCGGAAAGCAAATGGTATCCTGGGCTCCATCATGAGAGG GGTGGCCAGCAGGGACAGGGAGGTGATTGTCCCTGTCTACTCTGCTCTTGTGAGGCCCCATCTGGAGTAC TGTGTCCAGGTATGGAGCCCCCAGTACAAGAAAGACAGAGAGCTGTTGGAGAGGGTCCAGAGAAGGGCTA TGAAGATGATCAGCAGGCTGGAGTACCTCCCCTACGAGGACAGGCTGAGGGAGCTGGACTTATTCAGCCT GGAGAAGACAAGGCTGCGGGGTGACCTCATTGCAGCCTTTCAGTACCTGAAGGGAGCCTATAAACAGGAA GGGAGTAAACTCTCTGAAAGGGTAGATAACAGCAGGACAAGGGGGAACGGTTTTAAGTTGAAAGAGGGAA GATTTAGGTTGGATGTTAGGGGGAAGTTCTTTACCAGGAGAGCGGCGAGGTGCTGGAACGGGCTGCCCAG AGAGGTTGTGGATGCCCCATCCCTGGAGGTGTTCAAGGCCAGGCTGGATGAGGCCCTGGGCAACCCGGTC TAGTAAATGGGGAGGTTGGTGGCCCTTCCCGGCAGGGGGGTTGGAGATTCATGATCCTTGAGGTCCCTTC CAACCCAGGCCATTCTGTGATCTGTGAATTCTTAAATGCTTCAATCACTTCCCAGTATTAGATGCACATA TAGTAGGTATAACACTATAAATATATACTGTATCTAACAGTTTAAGTATCTATACATGTAAATATAGCAC TGGGCATATGTATATCTAGCACTTCACATATACACAGTCATGATATTGTAGATCATGTTACAACCGTAGG TAATTTATAAGATTACGATGGCAAATAGGTAGCCAGGGGAAAAATCTGTAGGATAGAAAATGGAACAAAA GATCTTTAATACTGTTCTTTCATTAAGGTAGCCTGCAGTGTGAGCAGATAGGAAAAGAGGATGAGTTTGT AATATTACGTTGTCCGATTTTCTGCTGTTTCAGAAAATGACTACAAGCTACAGATCTTTTGGTCCTTCAT TATAAACACAATAGGTAGCCTTGCCTTGATTGGATATCTTGTCAAATTGATCAAGTCAGTACTAAAAAAA AAAAAATCATATGAAATAAAATTATGACACACTATCTTCTTGGTTGTATCTTTCAGAAAACACATGTGAA AAGTATTTGTCTCCAGTACCAACTGTATCTACTTTTGAACAGCCATTTCTTTTGCCTTTTAAAGAATAAG ACTGGACTAACCATCTTCTTCCTATGGTAACTACAAAGTTAATATTAATGAGACAGTAAATACAAATTAT CTATTTTTTACTATTACAGTTGTTATTTGTAATGGCTTCCCATTGCCTTAGGAAGTGGATCCTCTCCTGC CTTGTTATGTAGTCCTACAATGCTCCTCAGGCCATGGTCCCTTGCTTTATTCTTTCTTTCTCTGAGCAGG CTCCATCCAGTTAATGTGTCTCAGCCTTTATCTCCATGATAATTCTCAAGTTGTTTGAACTGTTGCATTT GAAGATTTCCATATGCCTCTGCAGTGAAAAAAACAATGTGAACATGTTTAAATATGTGTGCTGGTTTATG TATATTAGATGTTTCCTTCTGCTCTGACTCCACTTTCCTGTCTTAGAAAACTGACCCTGACTCTAGCGCC AGCATCCCCTACTTGTTCTCTCCTTGTCAAATCTCTTTACTAAGGTCTAATGTTTCTGCTCTGTCTTCCT GGAAGAAAAAATCAGGGGTAAGTGTCCTCTGATTCTTGTTATAATACTTCTTTTATTTTTCTTTCCCAAA AATCTTGTTATCATTCAGCCTTTGTTTAAGGCCTAAATGGTGAACAGCTGCTTGTTTTATACTAACTTCT CAAATCAATTTCTTGTCTTCCTTTCATTGCCTTACATTACTTATAATACAGAAGTACTGAAAGGGATTGA AATGAAAGGTCAAAATGGGGACACGCTAAAACCATGGTTCACAGTGCAATAATCTTCCAGAGGAGAAAGA AATCTACCTCCTACCCTTTAGTGGTTCACTCCATGCAAAATATTATCCAGGGCAGGAAGAATCTTTTCTG TACACAAAAAATTACCTGTAAAATGACATACAATATTTCTACAGGGATAAAGACAGAAATTTCATAGTAG CAGGAGGAAAAATAATTCAGTATAAGAGAAATATACAAGAATGAAAATATACAAGAACAAAATTTGTTAT TTTGTTGTGATACTTGGAAAGAATTGTAGGTAATTTACAATAAATAATTGATTCTCTTGCATTACGTCTT TTAAAATAAGCTTTAAAACAAAACAAACAAACAAAAAAAAACCAACAACCTGAAAAACAACCTCATCTGT TTAAACAGTGTGGCTCTGTGATTTTACAAGTTGTCAAATGTACTTGCTACACACATTAACACACATATAC ATATCTTTAGTATCTGAATAATCGGTGATTCTTTTTTTCAGCATAAATAATGTGTGTACCTACTATTTAG CCTAGTAGTCACTGTTTTTCTTCTAGTGCTTATGTACCAAAGACAGAAGCAAATCACTGTAAGTGGTCAG ACGTTCTGAAAGATTTGGAGCTGATCAAGACATCTGAAGTAAGTTCAAAATAGTCACTATTTTATACAAT CATAGGAGCAGACATAACTACGTGTTTAGCACAGTACTCAGCTAAGAGAGTCAGTGACCAGGAGTATGAA ACAGGATATTGTCTGTGTAGTCATTTAGGGAATAAAAGCTAAAAGGAACTGGAGAGCTCTATTTTATAAT AGCTCTATTTTATGTTTTTTCAGAATTTCTTTAACTGGATAAAGCAAGAAGAAACTTTTCCTAAAGAAAG GGGCAGAGGGCATGGGAGCCCATCTTGCAGGTTACTAGGTGACTTTCAGATCACTGTCTCACTGTGTTCC AGTGCCTGGATTAATTGAACTGCTGAACTTGAATCCATGGCTCTAGTGTTTACTTCATTATTTCTAAGTC TGTTCATAATTACAACTCTTGTTTCCTTTTAGGACATTGATGTCAGTTTATATACTGCAAACACATACGA GGATGTAAGTATAGTTAAAATTTGGCTCAAACTGGTCTAGTACAGTAACTTCATTTCTTTACTAGCAACT TTTTCCAAAGGTAGACTTGCTCTAACTTGAGAAAAGCTTCAGAAACCAAATCATTTGCCTTGGAAGGCAG AGTTCTGCAAGTTAAATTACTTATGATGAATTATGTAAGCTGGTACTAAATAATTTTACTGAAGTAGCTG CTATTAAAATTACTCAACTAAAAGGGATGATCTAAAATGATTTTATCAAAGCAAACACTGAAAAATAATG TTGATACTCATTTGTAATTGGAGAAAGTGAAGGAGTGACAGGGAAAATATCTACTAAGATTATTATTTAA CACCATGCACTTGAATTCTATAATAAGAAAATGCTTGAGAAGCGCAATATGGAAAGGAACTGTCAGCATC TGCCAATACAATTCTCTGTTTTTACAGCCTAGAAACAAATAATATGTAATATCAAGAATTTAGCCTTATA ACTGATAGAGATTATGATTTATCCTACAAGCAATAAATTATGTTAATTTTTAATATAATTGTTAACTAAG TCATGGTTTCCATAAACTCATTCCTGAAGAGAATTTTTCATAATTTACTGTGGAATTCAGTCTTCACTCC ATGCAATCCTCTAAGGACATGAATCCAACCAATAACCTACTCCTAGCAGTTACTTTTAGTATAGCCCTGG TGCAACATGAGCTGGATTCATTGCTGTGGTGATGTTGGCAGTAGCCTGCTCCTGAGACAGCCTCCTCCCG ACAGCATAAACATTTACACAATGACTGTGCCGAATACACACAATACATTGACTCCTGTACGCAGAGTTAA TGAGATTTCCTAGAAATAATGGACTTATTGTATCTGTGCAAAATAGTCCCAGTGTGCATGCAGAATACAG TAACGTTGATGCATTCAGTGGACCTAGCAAGCCTGAGAAAGCAGACTCTAATGTGTCTGTGCAGTTAGCC CTGTACACAATAGAAAAAAAAGCTGCACAGCATACATGTCCATACTCACATACCCATTCTCTTGACTGAA ATGAGCAACTGAAATTAATACAGTATAAAGGCTAATTTATTAGCTGGTAATGTCAGGAAGATTTTCTTAA ATTGGAAAGGCTCGATGTGAAAATCAGGCAACCTGGGGCTTGTTCTTGGTTTTATTGTTGACTTGGTGAA AAAATGGTACCTCTGTGTTGCTGTTGTTGGTTTTTTTTAATTATTAAAATATGGGTAATATCTACTTTCT TTTTCTCCTCCACTCTCCATTATTCTGAATAATGAACTCTGAATATAAGTGCTAGAATTACTAAGAACAC TAATTTAATTCCATTAGTGTTAATTCTTTCCCATATTCCTATCCTAATTATTATTCCCATGTTCTTTGTC AAGACATAGTGTTCTGCTTTGTCATAGGAAATTGGCTGGCCTATGTGTCATATATTCGCTCTTAGTATAT TTTTGCAGATACTTAAAAGTATTCGAATAGTAAGAACAGATACTAAGTAAATAAAAATCACCACTGTCTG GTAAATTATATCCCACACTCACTTATCCCAATTTAATCTTAAAACTGCTATAACTTCCTTATATATTTTT TTTCCAGATAGAATGCCAGGAACCTGTAATGAGATGTTTTTTTTTAGAGATGAATGTGATTCTTCACGAA TGTGGTATCAAAAAATGTAGTAGGAAGCATGATGTACGGAACATATTGAAAAATGGAAATGCAAGATTTG CAACTTACCAGGTAAGTAAAACCAAAAGTCTGTGGATAAAATAGAAACGAGAGGTGTTTCTTCAGAATCA GAAATTCTGTTTCTCATACATCTGGAAGAGGCAAGATGGTACATATGAAAGCAAAAGGGAATCAACCAGT AACCCATGCACATAATACTGAGAAAGTTACACGTATAAAAAGGAAAGAGCAGTATTCAGTGATTTTAGAA AAACACTCCCAGGACTCATATCAAAGAAAATAGTTGTGACTGGGCACACATGCAGACATTAATGTTTGAA TGTGCATATATAAATAATAGTTTGTAACTTCAGTATCTAAACTCAGGTACGTTTAGTCACAAATAGAGAA GAGTCCCAATTTTCAAAGGGATAGGTGACTTAAACCTCTCACTGAAGCACTGTATACTTGAAAGATGATC CTATTCAGAAAACAAGCCAGGAACAAAACTTGAATACCTTGGAAGATTCCAGCAGTGCTTTCTGTAGCAA TCGACTGCATTGGAATTACTGACAAAAACAGAATAAAAAGGGTTAGCTCTCTATCAGATCAAATAGACAA AGTATCTTGCTTTACTATTGCATTTTCTTTTTCAGTTGAATTCCACAACATCAAAAAAATGCAAAGAATG TGAAGAGTATGAAGAAAAAAATTTTACAGAATTTATACAGAGTTTTGTAAAGGTTATACAGAGGGAATGC AAAAAATACATTAACTAAAATACGCAAGCTGGGAACAGTGACTTTTCAAGAAACATACCTCAGATAAATT AGATTGTCAATAACATTGAACTAAAAAGAACTTCACTTCTAGTCCTATTTGCACAGAATAAAAAAATAAA GAATAATAAAATCT >gi|45383037|ref|NP_989902.1| interleukin 15 [Gallus gallus] MLGMAQPTQNSAGARRRPESQKTHVKSICLQYQLYLLLNSHFFCLLKNKTGLTIFFLCAYVPKTEANHCK WSDVLKDLELIKTSEDIDVSLYTANTYEDIECQEPVMRCFFLEMKVILHECDIKKCSRKHDVRNIWKNGN ARFATYQLNSTTAKKCKECEEYEEKNFTEFIQSFVKVIQRECKKYAN Vaximmutor IL2 Gallus gallus 373958 49169785 AJ224516 NP_989484 9031 4 53134198 53137243 + interleukin 2 5.72 15833.1 143 Also known as IL-2 >gi|358485508:53134198-53137243 Gallus gallus breed Red Jungle fowl, inbred line UCD001 chromosome 4, Gallus_gallus-4.0, whole genome shotgun sequence TATCACCTCACATTGTACATCACAATTTGAATACCAGCATACAGATAACTGGGACACTGCCATGATGTGC AAAGTACTGATCTTTGGCTGTATTTCGGTAGCAATGCTAATGACTACAGCTTATGGAGCATCTCTATCAT CAGCAAAAAGGAAACCTCTTCAAACATTAATAAAGGATTTAGAAATATTGGAAAATATCAAGAACGTAAG TGGATGGTTTTCCTCTATCAGTTCTTTTCTGCTAATGTTTTCTACTTATTTTATCAATGCATTTTGTCTT CAAAAATTAACTTTTCCTCCCTCTTCTCTACAGAAGATTCATCTCGAGCTCTACACACCAACTGAGACCC AGGTGAGCTTTAGCCTTTTGTTTTCAGAAGGACTTCTAAGATATGGCTACTTTAAAATGATGTATTATTG ATTTAGACCAAAACAATTCACTTATCAGAAGGGACAATATAGAAAGCAAATGTGAGGTGCTTGAATTCAG TGTGAGGGTAGGATGGTTTCCTAAAATACACTTCTGCCTTTGATTTATATTCTTAAACATTAACAAGATA ATGAATGATATTACTTTCTTCGTCTGTGCAATACAAAACATACAACTAATCCTCTGTATTCATTTTGCTT ACCACACAGTTCAATTCACAAAAAAAAATGTTCTGTGAATCAATTATATGTTGTATAATATTTGCAGGAG GCAATACTGTTGTATCAACATTAGTATAAGAACAATTCTGAGAATGAAGGGTTAGAAACTTTTTAAATGC AGCAATATCCATAGGAAAATGCCCGTATTTTATCAAAACCAGATGCAGAGACAGTAGTTGGTTCTGTTTA ACAAGTTACAAATCTCCCACATCCCATCAATAATGAAACTTAATTATGCTCTAACTACCTCTTTCCAGAA GTAAAATGTAACCAACTCATCCTAGATATATTTAGCTGTAAATTCTTATTTTAAGGTGTTCTGTAAATAT GCAAGGCTTAGTTCCATGTCAATACCGTCACTGTGGTTTCTTTTTTTTTTTTCTAAAATACTGCATACCT GAGTCCAGGAGCATTCTAAGCACAGCATTATCACAGTCTATTTACCATTGACAGGTTGCTGACCTGAACA CTTTTTCTAATTTAGTGTGCAAGCAGCTAGCAAGAGTATCAATTTGTTTGTTACTGGGAAGTAGTTGAGA TACTTATGCAAGTTAACTACTTTGAGCCTTTAATTTAGTTTTTAATCCAACAGAATTCTCACATAACAGA AATTTTGTTTGAGTAAAGATTTAAAACCAGATTCTGCAACCACTTCCTTATCTGAGTCATTTTTCTTCTA TGCTGGCAAGATCAAGATGTAACTATCTCAGAGCTCCAGTGCTCAAATGCAGATGTGTAAGGCCAATTTA ATAGCATACTATCACCAAGTGCCTGAAAGAGCTTATAAGAGTGATGTGTAAAGCGATGTACCCATACTCA AGGTCAGGTAGCACACGACCCTGCCAAGGGCAAAGAGTTGGAATGAGATAGTCTATAAGGACTTCCTTCC AACCCAAGCAATTCTGTGCTTCTATGACATTCAACTGCAAGACCAGATATAGGCATTACATAAAGGAACC AAAGTGCATTTATTGAAGATTGCCACTGTAAAATAGAGGCTTTACTGGTAACTCTTTTCTTAATTTCCAC ACAAAGGATTTCCACACAACTGGAAAAAATGCTCAGTTTAAGAGCTAATACAGCAGCTTCATGTTCCCAA GAGATGTTCCATCATTTAAAATATCCTGTAAAGATTGTCTACATTTCAAAAGCTACAAGACACTAAGGCC ACTTGTAGAATCTTATAACGTGACTTGGAGGGAAAAATTGAACTATGTACTGTATAAAAGGGAAATCAAA TTCCTTCCTCATACAGGGAAAAAAGCAATGGGCAATTAAGAAAAAAAAAAATTCAAGTTTTTTCAGAGAA CGTTTTATATGTCCCTACTTAAGTGTTCCCGTGTCCCTACTGCAGGAGAGACTGGCAAAAATATTTTATA TAAATACTATATATATATTAATATGTATTGACTTTGATTACTGTATTAGGAGTGCACCCAGCAAACTCTG CAGTGTTACCTGGGAGAAGTGGTTACTCTGAAGAAAGAAACTGAAGATGACACTGAAATTAAAGAAGAAT TTGTAACTGCTATTCAAAATATCGAAAAGAACCTCAAGAGTCTTACGGTAGGTATTCTTTATGGTCAGTT AGTTAAAAACAAATGTGTTTCTGCAGTAATGAAAAAGAACATCAGAATCAGTCTTTTAGTTTTGCCTTAA GGCCTTTGTGTTATGGTTGCTTAAGAATATGGCATTTTGCACAAAAATCCTGTAATGTGAAACTACTTGA CAACATCTACTTGCATGAAAATCCAAAACTTTAAAACAGTTCTGAGCCAAACTAACTATACAAGTCAACA TTTTAAGAAGTATGAGATTTGTTTTCTTTGCTAAATCATGTAAGTCACCTTACAGTGCTTTTAGAAGGGT AGCAATTATTTACATAAGTAGTAGTTCAGAAAACTATGCCATAAATTCATGACTCTAAAACTATCTGGCA AGAGAGCTATCACCAATTAACCAAATTTATTGTACTGCAGGGTCTAAATCACACCGGAAGTGAATGCAAG ATCTGTGAAGCTAACAACAAGAAAAAATTTCCTGATTTTCTCCATGAACTGACCAACTTTGTGAGATATC TGCAAAAATAAGCAACTAATCATTTTTATTTTACTGCTATGTTATTTATTTAATTATTTAATTACAGATA ATTTATATATTTTATCCCGTGGCTAACTAATCTGCTGTCCATTCTGGGACCACTGTATGCTCTTAGTCTG GGTGATATGACGTCTGTTCTAAGATCATATTTGATCCTTTCTGTAAGCCCTACGGGCTCAAAATGTACGT TGGAAAACTGATTGATTCTCACTTTGTCGGTAAAGTGATATGTGTTTACTGAAAGAATTTTTAAAAGTCA CTTCTAGATGACATTTAATAAATTTCAGTAATATAT >gi|49169785|ref|NP_989484.1| interleukin 2 precursor [Gallus gallus] MMCKVLIFGCISVAMLMTTAYGASLSSEKWKTLQTLIKDLEILENIKNKIHLELYTPTETQECTQQTLQC YLGEVVTLKKETEDDTEIKEEFVTAIQNIEKNLKSLTGLNHTGSECKICEANNKKKFPDFLHELTNFVRY LQK Vaximmutor Huang et al., 2015 journal Huang J, Zhang Z, Li M, Song X, Yan R, Xu L, Li X 2015 44 5 392-400 Avian pathology : journal of the W.V.P.A Huang et al., 2015 journal Huang J, Zhang Z, Li M, Song X, Yan R, Xu L, Li X 2015 64 5 408-416 Parasitology international Jang et al., 2010 journal Jang SI, Lillehoj HS, Lee SH, Lee KW, Park MS, Cha SR, Lillehoj EP, Subramanian BM, Sriraman R, Srinivasan VA 2010 28 17 2980-2985 Vaccine Jang et al., 2010 journal Jang SI, Lillehoj HS, Lee SH, Lee KW, Park MS, Cha SR, Lillehoj EP, Subramanian BM, Sriraman R, Srinivasan VA 2010 28 17 2980-2985 Vaccine Smith et al., 1994 journal Smith NC, Wallach M, Miller CM, Morgenstern R, Braun R, Eckert J 1994 62 4 1348-1357 Infection and immunity Smith et al., 2002 journal Smith AL, Hesketh P, Archer A, Shirley MW 2002 70 5 2472-2479 Infection and immunity Wallach, 1997 journal Wallach M 1997 27 10 1159-1167 International journal for parasitology Witcombe et al., 2004 journal Witcombe DM, Ferguson DJ, Belli SI, Wallach MG, Smith NC 2004 34 7 861-872 International journal for parasitology Xu et al., 2013 journal Xu J, Zhang Y, Tao J 2013 51 2 147-154 The Korean journal of parasitology