Streptococcus equi 1336 S. equi enters via the mouth or nose and attaches to cells in the crypt of the tonsil and adjacent superficial lymphoid nodules. After a few hours, the organism is difficult to detect on the mucosal surface because it is translocated below the mucosa into the local lymphatics where it may be found in one or more of the lymph nodes that drain the pharyngeal/tonsillar region. Complement-derived chemotactic factors generated after interaction of C1 with bacterial peptidoglycan attract large numbers of PMN. The inability of PMN to phagocytose and kill the streptococci appears to be due to a combination of the hyaluronic acid capsule, antiphagocytic M protein, and a leukocidal toxin released by the organism. This culminates in accumulation of many extracellular streptococci in the form of long chains and large numbers of degenerating PMN. Final disposal of these organisms is dependent on lysis of the abscess capsule and evacuation of its contents (Timoney, 2004). Strangles (also equine distemper) Most horses develop a solid immunity during recovery from strangles which persists for up to five years in about 75% of animals. Acquired resistance appears to be primarily humoral and mediated by antibodies to SeM and other as yet unidentified antigens unique to S. equi (Timoney, 2004). S. equi primarily is a pathogen of equids, but can also cause disease in cattle, rabbits, swine, sheep and humans (MicrobeWiki: S. zooepidemicus). Mice can be used as a model of disease (Flock et al., 2006). Strangles (also equine distemper) is a contagious, upper respiratory tract infection of horses and other equines caused by a bacterium, Streptococcus equi var equi. The disease is spread when the nasal discharge or material from the draining abscess contaminates pastures, barns, feed troughs, etc. Clinical signs include fever, heavy nasal discharge, and swollen or enlarged lymph nodes in the neck and throatlatch. Affected animals may also stop eating and have a dull affect. Mortality may be up to 1 in 10 horses affected with strangles, especially bastard strangles. For cases without complications, mortality is usually lower. The disease is very contagious and morbidity is high. Precautions to limit the spread of the illness are necessary and those affected are normally isolated. An isolation period of 6 weeks is usually necessary to ensure that the disease is not still incubating before ending the isolation (Wiki: Strangles). Baboon Papio cynocephalus 9556 Bank vole Clethrionomys glareolus 447135 Bear Ursus americanus 9643 Birds Passeroidea 175121 Brown Trout Salmo trutta 8032 Buffalo Bison bison 9901 Carnivores Vulpes 9625 Cat Felis catus 9685 Catfishes Siluriformes 7995 Cattle Bos taurus 9913 Chicken Gallus gallus 9031 Chimpanzee Pan troglodytes 9598 chinchillas Chinchillidae 10150 Copper Pheasant Syrmaticus soemmerringii 9067 Deer Cervus elaphus 9860 Deer mouse Peromyscus maniculatus 10042 Dog Canis familiaris 9615 Ducks Anas 8835 Ferret Mustela putorius furo 9669 Fish Hyperotreti 117565 Gerbil Gerbillina 10045 Goat Capra hircus 9925 Gray wolf Canis lupus 9612 Guinea pig Cavia porcellus 10141 Hamster Mesocricetus auratus 10036 Horse Equus caballus 9796 Human Homo sapiens 9606 Macaque Macaca fascicularis 9541 Mongolian Gerbil Meriones unguiculatus 10047 Monkey Platyrrhini 9479 Mouse Mus musculus 10090 None None Parrot Psittacidae 9224 Pig Sus scrofa 9823 Rabbit Oryctolagus cuniculus 9986 Rainbow trout Oncorhynchus mykiss 8022 Rat Rattus 10114 Raven Corvus corax 56781 sei whale Balaenoptera borealis 9768 Sheep Ovis aries 9940 Squirrel Spermophilus richardsonii 37591 Tree shrew Tupaiidae 9393 Trouts, salmons & chars Salmoninae 504568 Turkey Meleagris gallopavo 9103 Vole Microtus ochrogaster 79684 Water buffalo Bubalus bubalis 391902 Equilis StrepE Equilis StrepE Intervet VO_0001054 Live, attenuated vaccine Licensed Intramuscular injection (i.m.) Live submucosal vaccine; deletions in aroA gene(Jacobs et al., 2000) Intramuscular injection (i.m.) S. equi Subunit CNE Protein Vaccine VO_0011546 Subunit vaccine Research Intranasally Matrix-S (Flock et al., 2006). Intranasally Recombinant protein preparation NMRI mice Fifteen to twenty microgram of each antigen and 10 μg of the adjuvant in a total volume of 12–16 μl were given to the mice by placing the antigens onto the nares and allowing the mice to inhale. This was done at three occasions at 1-week intervals. In each experiment, control animals were given only adjuvant at the same concentration omitting the antigen (Flock et al., 2006). Mice immunized with recombinant CNE lost less weight and had less nasal colonization than the unvaccinated controls (Flock et al., 2006). A dose containing 10^6 CFU in a 10-μl volume was used for infection of mice (Flock et al., 2006). S. equi Subunit EAG, FNZ and SFS Protein Vaccine VO_0011544 Subunit vaccine Research Subcutaneously The adjuvant used was EtxB, a recombinant form of the B subunit of Escherichia coli heat-labile enterotoxin (Flock et al., 2004). Subcutaneously Recombinant protein preparation Recombinant protein preparation Recombinant protein preparation Mice were immunized either subcutaneously (s.c.) or intranasally (i.n.). For each antigen (FNZ, SFS, and EAG), 12 μg of antigen was mixed with an equal amount of EtxB so that all components achieved a final concentration of 300 μg/ml. In the case of i.n. immunization, the total volume was kept to less than 40 μl and applied into the nostrils of anaesthetized mice . For both s.c. and i.n. immunizations, four doses were given with 1-week intervals (Flock et al., 2004). It was shown that nasal colonization of S. equi subsp. equi and weight loss due to infection were significantly reduced after vaccination compared with a mock-vaccinated control group. This effect was more pronounced after intranasal vaccination than after subcutaneous vaccination; nearly complete eradication of nasal colonization was obtained after intranasal vaccination (P < 0.001) (Flock et al., 2004). Challenge i.n. with S. equi subsp. equi (10^6 CFU in a 10-μl volume) was given 1 week after the last booster dose (Flock et al., 2004). An analysis of the bronchoalveolar lavage (BAL) fluid taken after the last immunization revealed significant IgA levels to EAG in the animals immunized with the three antigens and the EtxB adjuvant. By contrast, mice immunized with the three S. equi antigens in the absence of EtxB gave significantly lower IgA responses (Flock et al., 2004). Streptococcus Equi Live Culture Vaccine (USDA: 1831.00) Wyeth VO_0001784 Live vaccine Licensed USA Streptococcus equi vicK mutant vaccine VO_0002798 Live, attenuated vaccine Research intranasal immunization intranasal immunization Gene mutation This vicK mutant is from Streptococcus equi (Liu et al., 2008). A vicK mutant is attenuated in mice (Liu et al., 2008). A vicK mutant induces protection in mice from challenge with wild type S. equi (Liu et al., 2008). binding protein Eag Streptococcus equi subsp. equi 4047 VO_0011211 7696154 225870123 SEQ_0721 FM204883 YP_002746070 CDD:144825 553482 711797 713086 ? Ig, alpha2-macroglobulin and albumin binding protein Eag 4.57 41524.96 429 B domain; pfam01378 >NC_012471.1:711797-713086 Streptococcus equi subsp. equi 4047, complete genome AATGGAAAAAAACAAAAAGGTATCCTACTTTTTACGTCAATCAGCTGTTGGTTTAGCTTCAGTGTCGGCA GCCTTTCTGGTTGGAACGACTTCAGTGGGTGCTTTAGACGCAGCAACAGTGTTAGAGCCTACAACAGCCT TCATTAGAGAAGCTGTTAGGGAAATCAATCAGCTGAGTGATGACTACGCTGACAATCAAGAGCTTCAGGC TGTTCTTGCTAATGCTGGAGTTGAGGCACTTGCTGCAGATACTGTTGATCAGGCTAAAGCAGCTCTTGAC AAAGCAAAGGCAGCTGTTGCTGGTGTTCAGCTTGATGAAGCAAGACGTGAGGCTTACAGAACAATCAATG CCTTAAGTGATCAGCACAAAAGCGATCAAAAGGTTCAGCTAGCTCTAGTTGCTGCAGCAGCTAAGGTGGC AGATGCTGCTTCAGTTGATCAAGTGAATGCAGCCATTAATGATGCTCATACAGCTATTGCGGACATTACA GGAGCAGCCTTGTTGGAGGCTAAAGAAGCTGCTATCAATGAACTAAAGCAGTATGGCATTAGTGATTACT ATGTGACCTTAATCAACAAAGCCAAAACTGTTGAAGGTGTCAATGCGCTTAAGGCAGAGATTTTATCAGC TCTACCGAGTTCTGAGGTCATTGACGCAGCAGAACTAACACCAGCCTTGACTAGCTATAAGCTTGTCATC AAGGGAGCAACTTTTTCAGGTGAAACAGCTACTAAGGCAGTAGACGCAGCTGTAGCTGAGCAGACCTTCA GAGACTATGCTAATAAAAATGGTGTAGACGGCGTTTGGGCTTATGATGCTGCCACAAAGACATTTACAGT CACTGAACAGCCTGTAGCTGAGACTATTGAGGCGGCAGAATTAACACCAGCTTTGACCACCTATAGGCTT GTTATTAAGGGGGTTACCTTCTCAGGTGAAACAGCTACCAAGGCAGTAGACGCAGCCACAGCCGAGCAGA CCTTCAGGCAATATGCCAATGACAATGGCGTTACTGGTGAATGGGCTTATGACGCTGCTACAAAGACATT TACAGTCACTGAGGCTTTAGAGGAGAGTCCAGCTGATCCAGAAAAGCCATCAGCTTCTCTTCCGTTGACA CCGCTTACACCAGCAACTAAGACAGCACCAGCTAAGCAAAAAGATAAGCAAAAGGCTAAGACTCTTCCAA CGACTGGTGAGAAGGCTAATCCATTCTTTACAGCAGCGGCTCTTGCCATTATGGCGAGTGCAGGTGCTTT AGCAGTGACTTCAAAGCGTCAGCAGGACTA >YP_002746070.1 Ig, alpha2-macroglobulin and albumin binding protein Eag [Streptococcus equi subsp. equi 4047] MEKNKKVSYFLRQSAVGLASVSAAFLVGTTSVGALDAATVLEPTTAFIREAVREINQLSDDYADNQELQA VLANAGVEALAADTVDQAKAALDKAKAAVAGVQLDEARREAYRTINALSDQHKSDQKVQLALVAAAAKVA DAASVDQVNAAINDAHTAIADITGAALLEAKEAAINELKQYGISDYYVTLINKAKTVEGVNALKAEILSA LPSSEVIDAAELTPALTSYKLVIKGATFSGETATKAVDAAVAEQTFRDYANKNGVDGVWAYDAATKTFTV TEQPVAETIEAAELTPALTTYRLVIKGVTFSGETATKAVDAATAEQTFRQYANDNGVTGEWAYDAATKTF TVTEALEESPADPEKPSASLPLTPLTPATKTAPAKQKDKQKAKTLPTTGEKANPFFTAAALAIMASAGAL AVTSKRQQD Protective antigen Vaccination of mice with EAG (α2-macroglobulin, albumin, and immunoglobulin G [IgG] binding protein) as a single antigen with EtxB (as an adjuvant) induced protection in mice against bacterial challenge, but was less protective than at affording protection than immunization with FNZ, SFS, EAG, and EtxB [Ref1220:Flock et al., 2004]. The antigens used were EAG (alpha2-macroglobulin, albumin, and IgG-binding protein), CNE (a collagen-binding protein), and SclC (a collagen-like protein). The vaccinated horses showed significantly reduced bacterial growth (p=0.02).[Ref4703:Waller et al., 2007] collagen binding, ancillary pilus subunit Cne Streptococcus equi subsp. equi 4047 VO_0011213 7695900 225870316 SEQ_0935 FM204883 YP_002746263 CDD:283410 CDD:212461 CDD:273478 553482 914185 916158 ? collagen binding, ancillary pilus subunit Cne 6.61 68135.68 657 Collagen binding domain; pfam05737 >NC_012471.1:914185-916158 Streptococcus equi subsp. equi 4047, complete genome TTTGAAACAACTGACAAAGATCGTTAGTGTGGTCTTGTTGCTGGTCTTTACCCTTAGTGCTAGCCTGCAC AAGGTTCGGGCAACTAATCTTAGTGACAACATCACATCATTGACGGTTGCTTCTTCATCACTCCGAGATG GAGAGAGAACGACGGTAAAGGTTGCGTTTGATGACAAAAAACAGAAAATCAAGGCAGGGGATACGATAGA GGTCACCTGGCCTACAAGTGGTAATGTCTACATTCAGGGCTTTAATAAAACCATACCGCTTAATATTAGA GGGGTAGATGTTGGTACCTTGGAGGTCACGCTAGACAAGGCTGTTTTCACATTCAATCAAAATATTGAAA CAATGCATGATGTCTCTGGTTGGGGAGAGTTTGATATTACTGTTAGAAATGTGACACAAACCACCGCTGA AACATCAGGAACGACCACAGTAAAGGTAGGCAATCGCACTGCTACTATCACTGTTACTAAGCCTGAGGCA GGCACTGGTACCAGCTCATTTTATTATAAGACTGGTGATATGCAGCCCAATGATACTGAGCGTGTGAGAT GGTTCCTGCTGATTAACAACAACAAGGAATGGGTGGCCAATACTGTTACAGTCGAAGACGATATTCAAGG TGGTCAAACCTTGGATATGAGCAGCTTTGACATCACCGTATCTGGTTATCGTAACGAGCGCTTCGTTGGG GAAAACGCTCTGACAGAGTTTCATACAACATTTCCAAATTCTGTCATTACGGCAACAGATAATCACATTA GTGTGCGGTTAGATCAATATGATGCCTCACAAAACACTGTCAACATTGCTTATAAGACAAAGATAACGGA CTTTGACCAAAAAGAATTTGCCAACAACAGTAAAATCTGGTACCAGATTTTATACAAGGATCAGGTATCG GGTCAAGAGTCAAACCACCAAGTAGCCAATATCAATGCTAACGGCGGGGTTGATGGCAGTCGCTATACCA GCTTTACTGTCAAGAAAATTTGGAATGACAAGGAAAATCAAGACGGTAAGCGTCCAAAGACTATTACTGT TCAGCTTTACGCCAATGATCAGAAAGTTAATGATAAGACCATTGAATTGAGTGATACTAATAGCTGGCAA GCAAGTTTTGGTAAGCTGGATAAGTATGACAGTCAGAACCAAAAAATTACCTACAGTGTCAAGGAAGTGA TGGTTCCTGTTGGCTACCAATCGCAGGTTGAGGGGGATAGTGGAGTAGGATTTACCATTACCAACACCTA TACACCAGAGGTCATTAGCATTACCGGTCAAAAAACTTGGGACGACAGGGAAAACCAAGACGGTAAACGT CCTAAGGAGATTACGGTTCGTTTATTGGCAAATGACGCTGCAACTGACAAGGTAGCAACTGCTTCAGAGC AAACCGGCTGGAAGTATACATTTACCAATCTACCGAAATACAAAGATGGTAAACAGATCACCTACACGAT CCAAGAGGACCCTGTGGCAGATTACACCACAACCATTCAGGGATTTGATATTACCAATCATCATGAGGTA GCCTTGACCAGCCTAAAGGTCATCAAGGTTTGGAATGATAAGGACGATTATTACCATAAACGTCCCAAGG AGATTACCATTTTGCTAAAGGCAGATGGCAAGGTGATTCGTGAACATCAGATGACACCGGATCAGCAAGG AAAATGGGAATACACCTTTGACCAGCTGCCGGTCTATCAGACAGGCAAGAAAATCAGCTACAGCATTGAG GAAAAACAGGTTGCTGGCTATCAAGCCCCTGTCTATGAGGTTGATGAAGGCTTGAAGCAGGTCACTGTAA CCAACACCCTTAACCCAAGCTACAAGCTGCCTGACACCGGAGGACAAGGAGTGAAATGGTACCTGTTAAT CGGTGGCGGTTTTATCATCGTCGCAATCCTTGTACTGATCAGCCTTTATCAAAAACACAAGCGCCATAAC ATGTCAAAACCATA >YP_002746263.1 collagen binding, ancillary pilus subunit Cne [Streptococcus equi subsp. equi 4047] MKQLTKIVSVVLLLVFTLSASLHKVRATNLSDNITSLTVASSSLRDGERTTVKVAFDDKKQKIKAGDTIE VTWPTSGNVYIQGFNKTIPLNIRGVDVGTLEVTLDKAVFTFNQNIETMHDVSGWGEFDITVRNVTQTTAE TSGTTTVKVGNRTATITVTKPEAGTGTSSFYYKTGDMQPNDTERVRWFLLINNNKEWVANTVTVEDDIQG GQTLDMSSFDITVSGYRNERFVGENALTEFHTTFPNSVITATDNHISVRLDQYDASQNTVNIAYKTKITD FDQKEFANNSKIWYQILYKDQVSGQESNHQVANINANGGVDGSRYTSFTVKKIWNDKENQDGKRPKTITV QLYANDQKVNDKTIELSDTNSWQASFGKLDKYDSQNQKITYSVKEVMVPVGYQSQVEGDSGVGFTITNTY TPEVISITGQKTWDDRENQDGKRPKEITVRLLANDAATDKVATASEQTGWKYTFTNLPKYKDGKQITYTI QEDPVADYTTTIQGFDITNHHEVALTSLKVIKVWNDKDDYYHKRPKEITILLKADGKVIREHQMTPDQQG KWEYTFDQLPVYQTGKKISYSIEEKQVAGYQAPVYEVDEGLKQVTVTNTLNPSYKLPDTGGQGVKWYLLI GGGFIIVAILVLISLYQKHKRHNMSKP Protective antigen Mice were immunised i.n. with collagen-binding protein (CNE) and were subsequently subjected to infection with S. equi subsp. equi. A control group of mice was given only adjuvant. The CNE vaccinated group had a delayed onset of infection compared to the control group. Immunization with CNE led to significant protection from infection [Ref1217:Flock et al., 2006]. The antigens used were EAG (alpha2-macroglobulin, albumin, and IgG-binding protein), CNE (a collagen-binding protein), and SclC (a collagen-like protein). The vaccinated horses showed significantly reduced bacterial growth (p=0.02).[Ref4703:Waller et al., 2007] Fnz Streptococcus equi subsp. zooepidemicus VO_0011214 7695217 754170878 SZO_RS08670 FM204884 WP_041790586 40041 1860617 1862326 + thioester-forming surface-anchored protein 4.75 58938.07 569 Ortholog of S. equi 4047 (FM204883) SEQ0375 >NC_012470.1:1860617-1862326 Streptococcus equi subsp. zooepidemicus H70, complete genome TATGGAGAGAAGAGAGTTGAAAACAAAATCATTTAGAAAGGTACTGACGACGTCAGCTACCTGTATTGTG CTGGCAACAAGCTTTGCCGGAGGAACCCTACGCGTCTGGGCAGAGCAGCTTTATTATGGGTGGGATGATG GAACGAGACATGATTCACCCTCCTTTTTATATGTAACGCCTACTACTGAAAGTCAAAGAAGCCCCAAAAC TATTGTATACTGTTTTAATCGAAACAATTATTGGCCGGATAATTGGGAAACCCATGTCTTAAAACCAGAT AATCTACCTACTTATAATAAATTAACAGGAACTGCGAAGTTATTTCAAAATAAAGCTACCAAACCAAGAT CGGTAGCAGATATCGAGAAGTCCTTAGTTTCTGTCCTAAGTAAAGGGTATCCAACAAGTAATCTCTCTCA ATTTGGCATCAGTAATGAGTCAGCTAGAATAGTGACACAATTAGCCATTTGGTATTTTAGTGATAGTGTG AACGATCCTGAAAGCGTACTTAGACAAAGATTAGGGGGCAATGAATCTAAAGCATTAAAATACCTTATTG AGGTTGGTGAAAAACCACAATTGAGCGAAGCTGAGCAAAAGCAAACGTTAGATATATACGTTTACAACTC AGGAGGTTATCGTAGAACAGGTTACCAAAATCTTCTCGGCTCTACCTTAATTCCTAAAACACCAGAAAAG CCTTCTTCTGAATCTGTAACTCAGAGTAACCTTATTCTACTTGAACAGGAGAATCATTCTGGTACTATTT CAGGTGGTTCAGGAAATACTGAAACTGAGGAGAATACTCCTCATCTAATGGGGATAGGTGGCGGTCTAGC TGGTGAATCAGGAGAAAACACACCTAAACCAGGGGAAACTGGTGGGCACTCCCCTATAGTTGAAGAAAAC TACGGTAGTACTGAAGAATTTCATGGGAATTCAGAAATTATTAGTGAAACCGAAGATACTAATCCTGGTA TCATTTTAGGTGGCTCAGGAAATGTCGAAACACATGAAGATACTAGGAATCCTCATCTGATGGGGCTTGG CGGTGGTCTAGCTGGCGAATCAGGAGAAACGACACCTAAACCAGGACAGACTGGTGGTCATGGACCAATC ATCGAGACAACAGAGGACACACAAAAAGGCATGTCTGGGCAATCTGGCGGCACTATCGAGTCAGAAAACA CCAAAAAGCCTGAGGTCATGATTGGTGGGCAGGGACAAACCATCGAAACAACAGAGGACACACAAAAAGG CATGTCTGGGCAATCTGGCGGTACTATCGAGTCAGAAGACACTAAAAAACCTGAGGTCATGATTGGCGGT CAGGGACAAATCATCGACTTCTCTGAAAACACGCAATCAGGTATGTCTGGACAGTCTGGAGACACTACGG TCATTGAGGATACCAAGAAGTCTGAGATAATCATTGGTGGGCAAGGACAAATCATCGACTTCTCTGAGGA TACTCAGCCGGGTATGTCTGGGCAATCTGGAGGCACCACAATTGTCGAAGACACCAAGAAGCCGACACCT AAGCCTAAACCTGCACCTGCGCCAATTGTTAATGACGAAAAACCTAACAAAGGCACTCAGCTCCCACAGA CAAATGATATGAAGCAACTCACCCTAAGCATCATCGGTGCAATGTCAATGCTGCTTGTCCTATGTCTGTC TCTATTCAAGCGACCATCTAAAAAAGACTA >WP_041790586.1 thioester-forming surface-anchored protein [Streptococcus equi] MERRELKTKSFRKVLTTSATCIVLATSFAGGTLRVWAEQLYYGWDDGTRHDSPSFLYVTPTTESQRSPKT IVYCFNRNNYWPDNWETHVLKPDNLPTYNKLTGTAKLFQNKATKPRSVADIEKSLVSVLSKGYPTSNLSQ FGISNESARIVTQLAIWYFSDSVNDPESVLRQRLGGNESKALKYLIEVGEKPQLSEAEQKQTLDIYVYNS GGYRRTGYQNLLGSTLIPKTPEKPSSESVTQSNLILLEQENHSGTISGGSGNTETEENTPHLMGIGGGLA GESGENTPKPGETGGHSPIVEENYGSTEEFHGNSEIISETEDTNPGIILGGSGNVETHEDTRNPHLMGLG GGLAGESGETTPKPGQTGGHGPIIETTEDTQKGMSGQSGGTIESENTKKPEVMIGGQGQTIETTEDTQKG MSGQSGGTIESEDTKKPEVMIGGQGQIIDFSENTQSGMSGQSGDTTVIEDTKKSEIIIGGQGQIIDFSED TQPGMSGQSGGTTIVEDTKKPTPKPKPAPAPIVNDEKPNKGTQLPQTNDMKQLTLSIIGAMSMLLVLCLS LFKRPSKKD Protective antigen Parts of the three recombinant proteins (FNZ (cell surface-bound fibronectin binding protein), SFS (secreted fibronectin binding protein), and EAG (alpha2-macroglobulin, albumin, and immunoglobulin G [IgG] binding protein)) were used to immunize mice, either subcutaneously or intranasally, prior to nasal challenge with S. equi subsp. equi. The adjuvant used was EtxB, a recombinant form of the B subunit of Escherichia coli heat-labile enterotoxin. It was shown that nasal colonization of S. equi subsp. equi and weight loss due to infection were significantly reduced after vaccination compared with a mock-vaccinated control group. This effect was more pronounced after intranasal vaccination than after subcutaneous vaccination; nearly complete eradication of nasal colonization was obtained after intranasal vaccination (P < 0.001) [Ref1220:Flock et al., 2004]. IgA Mus musculus 238447 AC160982 10090 12 8607 12639 - immunoglobulin heavy constant alpha Also known as IgA; Igh-2 >gi|121699722:8607-12639 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12 AGTACTGGGGGACCTCTTTGCTGCCAAACGGGCCTCGAACAGTTGTAACAGTGAGTGCTGTGCTGTAGAA CAAGCTCAGTAGGAAGAGGGTGAGGAAGGTCACAGTGGTGGGCCACAGGCTGGCACCTGGGGCCTCTTCC TCCAGGATGTCTTCTGACTGGTCCAGTAGCACATAGGAAAGTGGCTCTTGACGTTCTGTAAGACATAGAT GACCTTCTAGATCCAAACCCATGGGGATGGGGGCTCAGACTTAAGGTTTCTGCTTCTATGCCTCCTTGGG CTTTGGGGACAGTGGTGGTAGGGGTCCCTTATTCCCGGGAAGGGACTTGTTCAGGGATAATGAGGAGGCA GGGTCCCAGGTAAGGGACTGGAATCCCAGCAGTTCTTGAGATGGCCCCAAGACCCATCTCTCTCTCTTAG GGAAACCTTAATTAGAGACCTGTCATGGTAAGAGAGTTGTTTCTGTCAAACTCTGAGAAGAGTTTGAACC CAAGGCTCCTTAGGTCTCAGTCCTTTCTAGATATTTAGCAATACCTCCCTAAACCCCAGTTCTTAGGCTA ATCTTGGCTTGGGTACTAGGGTTGTGTAAGATCAGACACCTTCAAACAGATACAGGCCAATGTGATTTCT GGGCCAAAGTTAGACTACCAAGGCCTGGACATGTGGTCAGAGGACTCTGTTGCATTCTTAAGAGCTGTGA CTGGGGGTTAAGCAACAGTAAAGACCCCCAGTAGAGCCAACCCTGCTGTCCTCAGGTTCTCTGCGCACAG AGTTTCATTTTAAGTGGCAGTAGGAAATGTGTACTGATAGTCAACCACTCGTATTTCTGTCACTGCAGCC ATCCATCAATCCATCCATCATCCCCATCGTTCATCCTTGTAGCCAGCCATCCATTACCCTCCAGCCGCTC ACTCATCATCTTTCCCGACTATCGTGATTCTCCAGCTTCTCTGCCCCCTCTCCCTGTGGGGATTCAGTCT TTCACCTATTGACTCCTCCTTACTAGGATTCTCTGTCATGGGCCAGGCCTTGGATTCCATGGGTGGCTAT GGTACTTTTCTGAGTCAGGTTTCCCAAGCTGGTCTGTTGCTCTTGCTATTCTCAGGGAACTGGACTTTCA AGCTGGAGAGTATCCAGGGCATGCATGGGCCTTTACTCCACTCTTGCTCTGAGAGCAGAGGGAAGTTGCT AGAAACCAGCAACTCCTCCCCCCCCCCCCCGAAGTTCTGAAGGCCTGTGAGACAGGAGCAGGGCCCCCAC TGCCCTCTTGTGGACACCTGGGCTCCAAGTATACCTGGTTGTAGAACTTTGTGTTCTAGGGGTGCCCATG GAGGAGGCCGAGGCCTGTGTGGGAGTGGTCTCCATGGCCTGCTCCTGCTTCTTCCCAGATTTGCCTCTTA TCCTGCTTCTGGTTTGTGATACTTGGGCTCAGGATGGGAAGGGAGACTTAGGTTAGAAAGGGGGCACCTG GAGGGGCCTATGCCCTTGTCCATTGTGTGTAGTTGTCAGTGTCGGGCCTGGAAATTCTCCCGGGGGCTTG GAGGGGACTCCCCTTGCTTTCCAGAAGCTGGGTGTGGGCTGTGAACTTCATCTTCCTCCAGGGTTCAGGT CTGTGTGAGTGTGCAAATGTGAGTGTCTGTGCATGTATGCCCATGTGCATGTATGCAGAACAGGGTGTAT GCCCATGTGCATGTGTGAAGAGCAGGGTGCTGCTGAGGGTTGTCTGTGCCATGCTATATGTCTTTCTCAT GAGTCAGTGGTCACCCTGAATGCATGTTTCCATTCAGGGATGGCCTACTGAGGGTGCGTGGCATCTTCTT CCCAGTGCCCCTGTGTTCATCCCTTCATTCTCATACCATCATCTCTATTGTGACCCCCACACGGCACCAG CCTTGATGGTGCTACCCTTTTGCTCCATCTATCCCTGTCTTGTTTGGGTTAGAAGCTGCGGTCTAATAAA CAGCCACACCACCCACCACTCAGAGTACTTGTGCAGCAACCCACTGCCCTGGGGACTCTCCCATGAGCTT TGCCTTCTTGGCTGAGTCTGCTCGCCTTGGATTTCATCCCCAGTTTATTCTTACTTCCCATTCACTCATT GCACATTCACAGGGCAGGTGCACACACTCTCATGTTTCCACCAACATGAACACACATAGATATATGCATG CTCATAGGTATGCACAGGCACACACTTGCTCACAGGGACATAGATATAGTGTGTCCACATATGTACTTAT ACCCATATACATACCTATAAACCATAGGTGCACACAAACTCACTCCTCTCTTGCTGCACACAGGTACACA CATGAAGGCACATGGACCCATCCATAAGCACTCACCAATACATAGACATGCATACACATATACTCTAGGC ATGAAGGGTCTTCAAAGTCTCCGAGGCCTTACAAGCTTAGGTGGTGGTGTTTCTCCCTCCCTGGGCCCTG ACCCCTCCCTGTGTTCTGTAGACCCTAAGGATGATGGACAGGCACTGGATGGAAGTGCAGGGATACTTTG GATGAGCACAGAGTTTATTTCAGGAGTAGGGACAGGCAGGGTGGCTCAGTAGCAGATGCCATCTCCCTCT GACATGATCACAGACACGCTGACATTGGTGGGTTTACCCGACAGACGGTCGATGGTCTTCTGGGTGAAGT TCATGGGCAAGGCCTCGTGGCCCACCATGCAGGAGTACTGGTCACCCTGTTTCCAGAGTTCAGCTGATAC ACGCAACACGCTTGTCACCAGGTAGGTGGTGGCTCCCTCGCCTGGCTCCTTTAGGGGCTCAAACACTAGG TAGCTTTCTGGGGACAGCTCCTCATTTCCATGCAGCCATCGCACCAGCACTTCTTTAGGGTTGAAAGCTC GCACCAGGCATGTCAGGGACACGAGCTCATTCAGGGCCAGCTCCTCCGACGGCGGCGGTAGCAGGTGGAC CTGGGGTGGGAAGGTGTTCACTGGAAGGTAGAAAGAAGAGTTATGGCTCAGACAAGGAGAGCAAGACCCC TCTGCCCTTCCCTTCTGGGCAGTTCCTCAGATAGCTCCAATTTCCCTCTGTGATATAAGGACAGGAGCAG TTAGTTGAGCATCTGTATTATAGGAAGAAAGCAGGCATGTGAGCAGGGAACGTCATACAATGTCCCGGGT ATGCATCTGGGCTCACCTGTGATTTTGGCAATTGTGCCAGTTAAGGTGTCAGACTCAGGATGGGTAACTG TGCACTTGAATGATGCGCCACTGTTCCAGCGCTCAGCACAGCCAGGCAGGACGCTGGACACACTGTAGCA GCCGCAGGAATTCTGCACAGCTTTCTTCTGCACTGCATCCTTCCCAGTGGAGGGCTCCCAGGTGAAGACA GCTCCCTCAGGATTTCTCAGGCCATTCAGAGTACATGTGAGGCTGGCATCTGAACCCAGGAGCAGGTCCT CAAGAGCTGGCCGCTGCAGTGACAGGCTGGGATGGCAGGAAGGAGGACAAGGAGGACAAGGAGGAGGAGG ACCTGTAACAGAGAAGTCCTAGCAAATCAGTATATTCTCCTTTTCTCCTTTGCTGTCCCCTTTTCTGGAT GTTCTGAGGCCTCAGTTCCTTATGGCCTGTTTACGCTTCCCTTCCCCCACCCCCATGGGAGGTTTCTGAG GGTCCCTGTGAGGGTGTGCCTCAGAGGGATATATGGATCTAGATAAGGTAGAACTTATCCCACCCCCAGC TGACCCCCTAACGTTCTTTACCAGAGCACTTCACATCCAATTCTTGGACGGCGTTAGAGTCATGTTGCAC GGAACATTTCACGGATTCTCCTTCTGGGCACTCGACAGCTGGCAGGGTCAACTGGCTGCTCATGGTGTAC CCTCCCCCAGAGGCCAGGGCAGGTGGGAAGTTTACGGTGGTTATATCCTTCCCACTCTTTCCCCAGGTCA CATTCATCGTGCCGGAAGGGAAGTAATCGTGAATCAGGCAGCCGATTATCACTGGGTCACTTGACAGAGC TCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGAC Vaximmutor Sfs Streptococcus equi subsp. equi 4047 VO_0011215 7696331 225869898 SEQ_0466 FM204883 YP_002745845 553482 452509 453624 ? fibronectin-binding protein Sfs 8.97 39068.51 371 Previously sequenced as Streptococcus equi fibronectin-binding protein Sfs UniProt:Q9X6F4 (EMBL:AF136451 (371 aa) fasta scores: E()=1.8e-153, 100.000% id in 371 aa >NC_012471.1:452509-453624 Streptococcus equi subsp. equi 4047, complete genome TATGAGAAAAACAGAAGGACGTTTTCGCACATGGAAGTCCAAAAAACAATGGCTATTTGCCGGTGCAGTG GTGACCAGCTTGCTGCTGGGAGCTGCACTTGTCTTTGGAGGTTTATTAGGAAGTCTTGGTGGCTCATCCC ATCAAGCGAGGCCAAAGGAGCAGCCAGTCAGCTCGATTGGAGATGACGATAAGTCGCACAAGAGCTCATC AGATTCAATGGTCAGTCGGCCACCGAAAAAGGATAACTTGCAGCCTAAGCCTTCAGATCAGCCTACTAAT CATCAGCATCAGGCTACTAGCCCGTCCCAGCCGACAGCAAAGAGCTCAGGTCATCATGGGAATCAACCTC AGTCTCTCAGTGTGAACAGCCAAGGAAATAGTAGTGGACAGGCCTCAGAGCCTCAGGCTATTCCTAATCA AGGGCCTTCCCAGCCACTGGGGCTGAGAGGAGGTAACAGCTCTGGTTCAGGTCATCACCATCAGCCACAA GGAAAGCCACAGCACTTAGATCTAGGTAAGGATAATTCTAGCCCGCAGCCTCAACCAAAGCCTCAGGGCA ATAGTCCCAAGCTGCCAGAAAAAGGCTTGAATGGTGAAAATCAGAAGGAACCGGAGCAAGGTGAACGAGG TGAGGCTGGTCCCCCACTTTCAGGGTTGAGTGGTAATAATCAAGGCCGTCCTTCGCTTCCAGGCTTGAAT GGTGAGAATCAGAAGGAACCAGAGCAAGGTGAACGAGGTGAAGCCGGTCCCCCATCAACTCCGAATTTAG AAGGAAATAATCGTAAAAATCCTTTAAAAGGATTAGATGGAGAGAATAAGCCAAAGGAAGATTTAGACGG TAAAGGCTTAAGCGGAGAAAATGATGAATCACCAAAACTTAAAGACGAACACCCCTACAATCATGGTCGT CGTGATGGTTACCGAGTTGGCTATGAGGATGGATATGGTGGCAAAAAGCACAAAGGAGATTATCCTAAGC GGTTTGATGAGTCTAGTCCAAAGGAATATAATGACTATAGTCAAGGGTATAATGATAATTATGGAAATGG CTATTTAGATGGTCTAGCTGATAGAGGTGGTAAGAGAGGATACGGCTATTCTTACAATCCCGACTA >YP_002745845.1 fibronectin-binding protein Sfs [Streptococcus equi subsp. equi 4047] MRKTEGRFRTWKSKKQWLFAGAVVTSLLLGAALVFGGLLGSLGGSSHQARPKEQPVSSIGDDDKSHKSSS DSMVSRPPKKDNLQPKPSDQPTNHQHQATSPSQPTAKSSGHHGNQPQSLSVNSQGNSSGQASEPQAIPNQ GPSQPLGLRGGNSSGSGHHHQPQGKPQHLDLGKDNSSPQPQPKPQGNSPKLPEKGLNGENQKEPEQGERG EAGPPLSGLSGNNQGRPSLPGLNGENQKEPEQGERGEAGPPSTPNLEGNNRKNPLKGLDGENKPKEDLDG KGLSGENDESPKLKDEHPYNHGRRDGYRVGYEDGYGGKKHKGDYPKRFDESSPKEYNDYSQGYNDNYGNG YLDGLADRGGKRGYGYSYNPD Protective antigen Parts of the three recombinant proteins (FNZ (cell surface-bound fibronectin binding protein), SFS (secreted fibronectin binding protein), and EAG (alpha2-macroglobulin, albumin, and immunoglobulin G [IgG] binding protein)) were used to immunize mice, either subcutaneously or intranasally, prior to nasal challenge with S. equi subsp. equi. The adjuvant used was EtxB, a recombinant form of the B subunit of Escherichia coli heat-labile enterotoxin. It was shown that nasal colonization of S. equi subsp. equi and weight loss due to infection were significantly reduced after vaccination compared with a mock-vaccinated control group. This effect was more pronounced after intranasal vaccination than after subcutaneous vaccination; nearly complete eradication of nasal colonization was obtained after intranasal vaccination (P < 0.001) [Ref1220:Flock et al., 2004]. vicK Streptococcus equi subsp. zooepidemicus MGCS10565 6760966 195978500 Sez_1395 CP001129 YP_002123744 552526 1433586 1434938 - TCS sensor kinase VicK 5.4 49245.51 450 similar to ref|YP_600051.1| Two-component sensor histidine kinase VicK [Streptococcus pyogenes MGAS2096] >gi|195977156:1433586-1434938 Streptococcus equi subsp. zooepidemicus MGCS10565, complete genome ATTAATCAATATCATCCTCCCATTCATCATAGATAGCAGCATCCTTTTCATAAGGTAACACAATGGTAAA GGTTGAGCCCTTGCCATAATCACTCTTAGCCCAAATAAAGCCTCGGTGCTGCTTAATAATTTCTTTAGCA ATCGCTAGGCCTAGCCCGGTCCCTCCTTGAGCTCTACTCCTAGCCTTATCCACTCGGTAAAAACGGTCAA AAATCAAAGGCAGGTCCTTTTTTGGAATACCCAAGCCCTCATCAGAAATCGAAATGATTAGCTGGGTATC TGTTGTTTTCATGTTGACGACAATTTTTCCGCCATCGGGTGAATATTTGATCGCATTGTTTAAAATATTT TCGATAACCTGCGTCATTTTGTCATCGTCAATTTCTAGCCAAATAGAGGTAATGGGGTAATCTCTGACAA TTTCATAGGATTTTCCTGTGCTTGTGTGCTGATTTTTCACCAAATCAAAGCGGTTTAAGATAGAGGTCAT AAAGGCTGTAAAGTTGGTCATTTCAACAGACAGCTGTGTCACATGATTATCAATTCTTGAGAGGTTGAGC AAATCTGAAATCATGCGCATCATGCGATTGGTTTCATCTAGTGAGACCTTTATAAAGCTTGGAGCGATGT CCTCCTTTAGCGCGCCCTCATCTAGAGCCTCTAGGTATGACTTGACAGAGGTCAAAGGTGTCCGCAGCTC ATGGCTAACATTTGAAACAAAGAGTCTGCGCTCGCGCTCTTCCTTTTCCTGCTCAGTCGTGTCATGAAGT ACAACAACCAGACCTGAAATAAAGCCGCTCTCTCGCCTATTAAGCGCAAAGCGCAGCCGCAGGGAAACAA ATTCTCCTGTATCATTACGACGGTTTAAGGTCACAATCGGTGTTTTTGAGACCAGCTCTCGGTAGGTATA AGGGGAGTCACTGTCTAATAAATCTGTAATGTTTTTTTCCAAGGCCTGCTCGCGATTTAAATTGAGCTGC TTTTGAGCAGTCTCATTAATCATGATAATCTTGCCGGAACGATTGGTCGCAAGGACCCCATCTGTCATAT AAGCCAAAATACTAGCAAGACGATTTTTTTCTTGAGCAAGGTTTTCATGAGTCAGACGAAAAACATCTGA TAGGTCATTGAGCTGCTCAGACAGCTCCATCAATTCAATATCAGCCTTTTCATTGATCGTATCAGTATAG CGACCATTGATCAAATCTCTTATCTTGTGGCTCATCAGACGAATGATCCGAGCATTGCGATAATCACGAA TGGCTAAATGAATGAAATAAAAAGCAACAAAAACAAGCAATAAGAGTATGGCTAGCTCAAATGCAGAAAG GTTTCCAATGATGTCCTTAGTCA >gi|195978500|ref|YP_002123744.1| TCS sensor kinase VicK [Streptococcus equi subsp. zooepidemicus MGCS10565] MTKDIIGNLSAFELAILLLLVFVAFYFIHLAIRDYRNARIIRLMSHKIRDLINGRYTDTINEKADIELME LSEQLNDLSDVFRLTHENLAQEKNRLASILAYMTDGVLATNRSGKIIMINETAQKQLNLNREQALEKNIT DLLDSDSPYTYRELVSKTPIVTLNRRNDTGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERR LFVSNVSHELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNHVTQLSV EMTNFTAFMTSILNRFDLVKNQHTSTGKSYEIVRDYPITSIWLEIDDDKMTQVIENILNNAIKYSPDGGK IVVNMKTTDTQLIISISDEGLGIPKKDLPLIFDRFYRVDKARSRAQGGTGLGLAIAKEIIKQHRGFIWAK SDYGKGSTFTIVLPYEKDAAIYDEWEDDID Virmugen A vicK mutant is attenuated in mice and induced protection from challenge with wild type S. equi [Ref1650:Liu et al., 2008]. 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