Yersinia ruckeri 29486 Enteric redmouth disease Yersinia ruckeri is the causative agent of yersiniosis or enteric redmouth disease leading to significant economic losses in salmonid aquaculture worldwide. Infection may result in a septicaemic condition with haemorrhages on the body surface and in the internal organs. Despite the significance of the disease, very little information is available on the pathogenesis, hampering the development of preventive measures to efficiently combat this bacterial agent (Tobback et al., 2007). Baboon Papio cynocephalus 9556 Bank vole Clethrionomys glareolus 447135 Bear Ursus americanus 9643 Birds Passeroidea 175121 Brown Trout Salmo trutta 8032 Buffalo Bison bison 9901 Carnivores Vulpes 9625 Cat Felis catus 9685 Catfishes Siluriformes 7995 Cattle Bos taurus 9913 Chicken Gallus gallus 9031 Chimpanzee Pan troglodytes 9598 chinchillas Chinchillidae 10150 Copper Pheasant Syrmaticus soemmerringii 9067 Deer Cervus elaphus 9860 Deer mouse Peromyscus maniculatus 10042 Dog Canis familiaris 9615 Ducks Anas 8835 Ferret Mustela putorius furo 9669 Fish Hyperotreti 117565 Gerbil Gerbillina 10045 Goat Capra hircus 9925 Gray wolf Canis lupus 9612 Guinea pig Cavia porcellus 10141 Hamster Mesocricetus auratus 10036 Horse Equus caballus 9796 Human Homo sapiens 9606 Macaque Macaca fascicularis 9541 Mongolian Gerbil Meriones unguiculatus 10047 Monkey Platyrrhini 9479 Mouse Mus musculus 10090 None None Parrot Psittacidae 9224 Pig Sus scrofa 9823 Rabbit Oryctolagus cuniculus 9986 Rainbow trout Oncorhynchus mykiss 8022 Rat Rattus 10114 Raven Corvus corax 56781 sei whale Balaenoptera borealis 9768 Sheep Ovis aries 9940 Squirrel Spermophilus richardsonii 37591 Tree shrew Tupaiidae 9393 Trouts, salmons & chars Salmoninae 504568 Turkey Meleagris gallopavo 9103 Vole Microtus ochrogaster 79684 Water buffalo Bubalus bubalis 391902 AquaVac ERM AquaVac ERM Schering-Plough Animal Health VO_0000842 Inactivated or "killed" vaccine Licensed Intramuscular injection (i.m.) Killed oral vaccine Intramuscular injection (i.m.) Yersinia ruckeri aroA mutant vaccine VO_0002948 Live, attenuated vaccine Research Intraperitoneal injection (i.p.) Intraperitoneal injection (i.p.) Gene mutation The aroA gene is mutated in Yersinia ruckeri (Temprano et al., 2005). An aroA mutant is attenuated in rainbow trout (Temprano et al., 2005). An aroA mutant provided significant protection from challenge with wild type Y. ruckeri (Temprano et al., 2005). aroA 15420539 CDD:179423 CDD:30129 29486 ? 5-enolpyruvylshikimate-3-phosphate synthase 427 3-phosphoshikimate 1-carboxyvinyltransferase; Provisional >gi|15420539|gb|AAK97382.1|AF360730_1 5-enolpyruvylshikimate-3-phosphate synthase [Yersinia ruckeri] MLESLTLQPIALINGTVNLPGSKSVSNRALLLAALAEGTTRLHNLLDSDDIRHMLNALKSLGVNYRLSAD RTQCDVDGLGGPLVADKTLELFLGNAGTAMRPLAAALCLGYSDIVLTGEERMKERPIGHLVDALEGGAQI DYLEQENYPPLRIRGGFRGGELTVDGSVSSQFLTALLMAAPLATQDTHIRIQGDLVSRPYIDITLHLMRS FGVEVTHQNYQVFHIQGGQTYHSPGEYLVEGDASSASYFLAAAAIKGGTVRVTGIGKKSVQGDTKFADVL EKMGAIIHWGDDYIECSRGELRDIDMDMNHIPDAAMTIATAALFAKGPTIIRNIYNWRVKETDRLSAMAT ELRKVGAEVEEGQDYIRVVPPAHLIAAEIGTYNDHRMAMCFSLVALSDTPVTILDPKCTAKTFPDYFEQL ARLSVLA Virmugen An aroA mutant is highly attenuated in rainbow trout and induces significant protection from challenge with wild type Y. ruckeri [Ref1659:Temprano et al., 2005]. Temprano et al., 2005 journal Temprano A, Riaño J, Yugueros J, González P, de Castro L, Villena A, Luengo JM, Naharro G 2005 28 7 419-427 Journal of fish diseases Tobback et al., 2007 journal Tobback E, Decostere A, Hermans K, Haesebrouck F, Chiers K 2007 30 5 257-268 Journal of fish diseases Yang et al., 2009 journal Yang H, Chen J, Yang G, Zhang XH, Liu R, Xue X 2009 27 15 2150-2155 Vaccine