Bovine herpesvirus 1 10320 Infectious bovine rhinotracheitis Bovine herpesvirus 1 (BHV-1) is a virus of the family Herpesviridae that causes several diseases worldwide in cattle, including rhinotracheitis, vaginitis, balanoposthitis, abortion, conjunctivitis, and enteritis. BHV-1 is also a contributing factor in shipping fever. It is spread through sexual contact, artificial insemination, and aerosol transmission. Like other herpesviruses, BHV-1 causes a lifelong latent infection and shedding of the virus. The sciatic nerve and trigeminal nerve are the sites of latency. There is a vaccine available which reduces the severity and incidence of disease. The respiratory disease caused by BHV-1 is commonly known as infectious bovine rhinotracheitis. Symptoms include fever, discharge from the nose, cough, difficulty breathing, and loss of appetite. Ulcers commonly occur in the mouth and nose. Mortality may reach 10%. The genital disease causes infectious pustular vulvovaginitis in cows and infectious balanoposthitis in bulls. Symptoms include fever, depressios, loss of appetite, painful urination, a swollen vulva with pustules and discharge in cows, and pain on sexual contact in bulls. In both cases lesions usually resolve within two weeks. Abortion and stillbirths can occur one to three months postinfection. BHV-1 also causes a generalized disease in newborn calves, characterized by enteritis and death (Wiki: Bovine herpesvirus 1). Baboon Papio cynocephalus 9556 Bank vole Clethrionomys glareolus 447135 Bear Ursus americanus 9643 Birds Passeroidea 175121 Brown Trout Salmo trutta 8032 Buffalo Bison bison 9901 Carnivores Vulpes 9625 Cat Felis catus 9685 Catfishes Siluriformes 7995 Cattle Bos taurus 9913 Chicken Gallus gallus 9031 Chimpanzee Pan troglodytes 9598 chinchillas Chinchillidae 10150 Copper Pheasant Syrmaticus soemmerringii 9067 Deer Cervus elaphus 9860 Deer mouse Peromyscus maniculatus 10042 Dog Canis familiaris 9615 Ducks Anas 8835 Ferret Mustela putorius furo 9669 Fish Hyperotreti 117565 Gerbil Gerbillina 10045 Goat Capra hircus 9925 Gray wolf Canis lupus 9612 Guinea pig Cavia porcellus 10141 Hamster Mesocricetus auratus 10036 Horse Equus caballus 9796 Human Homo sapiens 9606 Macaque Macaca fascicularis 9541 Mongolian Gerbil Meriones unguiculatus 10047 Monkey Platyrrhini 9479 Mouse Mus musculus 10090 None None Parrot Psittacidae 9224 Pig Sus scrofa 9823 Rabbit Oryctolagus cuniculus 9986 Rainbow trout Oncorhynchus mykiss 8022 Rat Rattus 10114 Raven Corvus corax 56781 sei whale Balaenoptera borealis 9768 Sheep Ovis aries 9940 Squirrel Spermophilus richardsonii 37591 Tree shrew Tupaiidae 9393 Trouts, salmons & chars Salmoninae 504568 Turkey Meleagris gallopavo 9103 Vole Microtus ochrogaster 79684 Water buffalo Bubalus bubalis 391902 BHV1 gD subunit vaccine VO_0004225 Subunit vaccine Research Intramuscular injection (i.m.) Intramuscular injection (i.m.) 25 μg of vaccinia recombinant gD (van et al., 1997). All vaccinated calves had significantly (P<0.05) higher serum neutralizing antibody titers prechallenge than the control animals. Calves immunized with the experimental gD or tgD vaccines had significantly (P<0.05) higher antibody titers than animals immunized with KV or MLV vaccines (van et al., 1997). Calves were randomly allocated to one of five vaccine groups and immunized with either 25 &mu;g of gD in avridine, 25 &mu;g tgD in avridine, commercial KV, commercial MLV or avridine. All vaccines were administered intramuscularly. 3 weeks after primary immunization the calves were re-immunized (van et al., 1997). All vaccinated calves had a significantly lower (P<0.05) rectal temperature and sick score than the placebo group. In contrast to the placebo-, KV- and MLV-immunized groups, the gD- and tgD-immunized groups experienced minimal weight loss during the period following challenge. The gD- and tgD-vaccinated calves shed significantly (P<0.05) lower amounts of virus than the placebo or KV-immunized groups throughout the follow-up period. In the gD and tgD subunit vaccine vaccinated groups only one out of eight animals shed virus (van et al., 1997). Calves were challenged 4 weeks after secondary immunization with BHV1. Each calf was exposed for 4 minutes to an aerosol of 10^7 p.f.u. ml- ’ of BHVl strain 108 (van et al., 1997). Bovilis IBR Marker Bovilis IBR Marker Intervet VO_0000979 Live, attenuated vaccine Licensed Intramuscular injection (i.m.) Live or inactivated gE-deleted marker vaccine(van et al., 1996) Intramuscular injection (i.m.) Bovine herpesvirus 1 DNA vaccine AdCMVgC VO_0011382 DNA vaccine Research pCi (Promega) Intranasal Intranasal Bovine herpesvirus 1 glycoprotein C DNA vaccine construction For the gC gene, a SpeI–NotI fragment (containing the gC gene) was extracted from pSKgC and cloned into pCi to yield plasmid pCigC. The expression cassettes containing the CMV promoter, splicing signals, glycoprotein gene, and SV40 polyadenylation signal, were extracted as BamHI–BglII fragments that were cloned into the BamHI site of plasmid pAd-link, resulting in plasmid pAdCMVgC (Gogev et al., 2002). Twenty-eight calves, 6–8 months old, were randomly allocated into five groups of five calves and one group of three calves. Three groups were inoculated twice 3 weeks apart by the intranasal route with 2 ml per nostril with a semi-purified preparation at a dose of 1010 CCID50 (Cell Culture Infectious Dose) of either AdCMVgC, or AdCMVgD or a combination of these two recombinants (5×10^9 CCID50 of AdCMVgC+5×10^9 CCID50 of AdCMVgD). At days 0 and 21, one group was inoculated twice by the intranasal route with a commercially available live vaccine whereas the group of three animals was inoculated intranasally twice 3 weeks apart with inactivated AdCMVgD, inactivated AdCMVgC, and a combination of them. One group was not inoculated and served as control (Gogev et al., 2002). The administration of either Ad5CMVgD or Ad5CMVgC, or a combination of them in calves by intranasal route 3 weeks apart, induced BHV-1 neutralizing antibody responses and conferred protection against challenge with BHV-1 Iowa strain (Gogev et al., 2002). On day 42, calves were challenged by the intranasal route with 5×10^6 plaque forming units (PFU) of BHV-1 IOWA strain. Calves were clinically examined and rectal temperatures were measured for 2 weeks after each immunization and for 3 weeks following the challenge performed on day 42 (Gogev et al., 2002). Bovine herpesvirus 1 DNA vaccine AdCMVgD VO_0011381 DNA vaccine Research pCi (Promega) Intranasal Intranasal Bovine herpesvirus 1 glycoprotein D DNA vaccine construction For the gD gene, a NheI–NotI fragment (containing the gD gene) was extracted from pGEMgD and cloned into pCi to yield plasmid pCigD. The expression cassettes containing the CMV promoter, splicing signals, glycoprotein gene, and SV40 polyadenylation signal, were extracted as BamHI–BglII fragments that were cloned into the BamHI site of plasmid pAd-link, resulting in plasmid pAdCMVgD (Gogev et al., 2002). Twenty-eight calves, 6–8 months old, were randomly allocated into five groups of five calves and one group of three calves. Three groups were inoculated twice 3 weeks apart by the intranasal route with 2 ml per nostril with a semi-purified preparation at a dose of 10^10 CCID50 (Cell Culture Infectious Dose) of either AdCMVgC, or AdCMVgD or a combination of these two recombinants (5×10^9 CCID50 of AdCMVgC+5×10^9 CCID50 of AdCMVgD). At days 0 and 21, one group was inoculated twice by the intranasal route with a commercially available live vaccine whereas the group of three animals was inoculated intranasally twice 3 weeks apart with inactivated AdCMVgD, inactivated AdCMVgC, and a combination of them. One group was not inoculated and served as control (Gogev et al., 2002). The administration of either Ad5CMVgD or Ad5CMVgC, or a combination of them in calves by intranasal route 3 weeks apart, induced BHV-1 neutralizing antibody responses and conferred protection against challenge with BHV-1 Iowa strain (Gogev et al., 2002). On day 42, calves were challenged by the intranasal route with 5×10^6 plaque forming units (PFU) of BHV-1 IOWA strain. Calves were clinically examined and rectal temperatures were measured for 2 weeks after each immunization and for 3 weeks following the challenge performed on day 42 (Gogev et al., 2002). Bovine herpesvirus 1 DNA vaccine pMASIAtgB encoding a truncated, secreted form of gB VO_0004317 DNA vaccine Research pMASIA [Ref1034:Huang et al., 2005] Intraperitoneal injection (i.p.) Intraperitoneal injection (i.p.) DNA vaccine construction Vector pMASIA expressed truncated, secreted form of BHV-1 gB (Huang et al., 2005). pMASIAtgB elicited both humoral responses and activated gamma interferon-secreting CD8+ CTLs, suggesting that a DNA vaccine expressing tgB induces a CTL response in the natural host of BHV-1 (Huang et al., 2005). VO_0000286 Bovine herpesvirus 1 DNA vaccine pRSV-gC VO_0011557 DNA vaccine Research pRSV containing a RSV promoter/enhancer Intramuscular injection (i.m.) Intramuscular injection (i.m.) Bovine herpesvirus 1 glycoprotein C DNA vaccine construction The complete coding sequence of the gene for gC was excised from a recombinant plasmid containing HindIII ‘I’ fragment of BHV-1 DNA (Gupta et al., 1995). The 2.4 kb BamHI and EcoRI double digested DNA fragment containing gC gene of BHV-1 was subcloned into a pRSV vector. The recombinant plasmid (pRSV-gC) encoding gC under the control of a RSV promoter/enhancer yielded high levels of BHV-1 gC expression in transfected cell (Gupta et al., 1998). Plasmid DNA was prepared from Escherichia coli bacterial cultures by the alkali lysis method and passed over Qiagen plasmid purification columns (Qiagen, CA). The isolated plasmid was ethanol-precipitated and dissolved in 0.85% saline at a concentration of 0.5 mg/ml (Gupta et al., 2001). Three calves were inoculated intramuscular (i.m.) six times with 500 μg of pRSV-gC and two calves were inoculated i.d. six times with 250 μg of pRSV-gC at each immunization. One calf received saline at each immunization and served as unvaccinated control. All inoculations were done at monthly intervals (Gupta et al., 2001). Results indicate that DNA immunization with gC could induce neutralizing antibody and lymphoproliferative responses with BHV-1 responsive memory B cells in bovines. However, the immunity developed sufficient for only partial protection against BHV-1 challenge (Gupta et al., 2001). All calves were exposed to BHV-1 (108.5 TCID50) by intranasal aerosol installation 1 month after last injection. All calves were examined daily for clinical symptoms of the disease. Nasal swabs were collected before challenge and 1, 3, 5, 7, 9 and 11 days post-challenge for isolation of shedding virus (Gupta et al., 2001). Bovine herpesvirus 1 DNA vaccine pRSVgIV VO_0004315 DNA vaccine Research pRSV [Ref2145:Cox et al., 1993] Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction Vector pRSV expressed BHV-1 glycoprotein I (gIV) (Cox et al., 1993). VO_0000286 Clinical symptoms including fever, nasal mucosal lesions, and inappetence were reduced in all pRSVgIV-injected calves compared with the pRSVO-injected calf (control). Increases in gIV-specific antibody titers were observed at day 6 post challenge and continued to the end of the experiment. By comparison with the titer achieved by the control calf after challenge, it is clear that all three pRSVgIV-injected calves were primed to respond to BHV-1 (Cox et al., 1993). Bovine herpesvirus 1 DNA vaccine tgD-VP22 VO_0011554 DNA vaccine Research pMASIA-tgD [Ref1028:Zheng et al., 2005] Intradermal injection (i.d.) Intradermal injection (i.d.) Bovine herpesvirus 1 tegument protein VP22 and glycoprotein D DNA vaccine construction Researchers constructed the plasmids pVP22-YFP, pMASIA-tgD-YFP, and pMASIA-tgD-VP22-YFP. For the construction of pVP22-YFP, the UL49 (VP22 gene) open reading frame was amplified from BHV-1 genomic DNA by PCR and then inserted into pEYFP-N1 (Clontech, BD Biosciences, Palo Alto, Calif.). Subsequently, pMASIA-tgD-YFP and pMASIA-tgD-VP22-YFP were generated by PCR cloning of the YFP and VP22-YFP genes from pEYFP-N1 and pVP22-YFP, respectively, into pMASIA-tgD, which encodes BHV-1 tgD (26). COS-7 cells were transfected with pMASIA-tgD-VP22-YFP, pMASIA-tgD-YFP, and pVP22-YFP and monitored every 4 h by fluorescence microscopy (Zheng et al., 2005). DNA vaccine construction Researchers constructed the plasmids pVP22-YFP, pMASIA-tgD-YFP, and pMASIA-tgD-VP22-YFP. For the construction of pVP22-YFP, the UL49 (VP22 gene) open reading frame was amplified from BHV-1 genomic DNA by PCR and then inserted into pEYFP-N1 (Clontech, BD Biosciences, Palo Alto, Calif.). Subsequently, pMASIA-tgD-YFP and pMASIA-tgD-VP22-YFP were generated by PCR cloning of the YFP and VP22-YFP genes from pEYFP-N1 and pVP22-YFP, respectively, into pMASIA-tgD, which encodes BHV-1 tgD (26). COS-7 cells were transfected with pMASIA-tgD-VP22-YFP, pMASIA-tgD-YFP, and pVP22-YFP and monitored every 4 h by fluorescence microscopy (Zheng et al., 2005). Calves were immunized intradermally with 500 μg of pMASIA-tgD-VP22, 500 μg of pMASIA-tgD, or saline in a 500-μl volume by use of the Biojector 2000 needle-free injection system (Bioject, Bedminster, N.J.) (Zheng et al., 2005). Protection against a BHV-1 challenge was obtained in calves immunized with the plasmid encoding tgD-VP22 (UL49), as shown by significant reductions in viral excretion (Zheng et al., 2005). All animals were reimmunized after 28 days and were challenged with BHV-1 strain 108 on day 54. All data from this study were analyzed with the aid of Graphpad Prism 3.0 (Zheng et al., 2005). Bovine herpesvirus 1 GPI protein vaccine VO_0011383 Subunit vaccine Research Bovine herpesvirus 1 envelope glycoprotein I Recombinant protein preparation A truncated BHV-1 envelope gpI protein was secreted into the culture supernatant of D17 cells transfected with the gpI gene lacking the coding sequence for the transmembrane region (TMR). The transmembrane domain is essential for gpI stability in the envelope, virus infectivity and, most probably, natural killer cell recognition (Gao et al., 1994). The 20 calves were divided into two groups (ten animals in each group) and immunized as follows:group 1 was primed intramusculary (i.m.) with concentrated gpI (17 ug/animal) emulsified in complete Freund's adjuvant, and boosted by intranasal (i.n.) aerosolization (Laboratory spray unit, Gelman Sciences Inc., Ann Arbor, MI) with 100yg gpI plus 20 ug cholera toxin subunit B (CTB) per animal at the 3rd and 9th weeks. Then, 30 yg of gpI emulsified in incomplete Freund's adjuvant was administered subcutaneously at the base of the ear at the 15th week. Alternatively, group 2 was treated as above with the same amount of non-transfected D17 cell culture supernatant concentrated from a volume equal to that of the gpI supernatant. The antibody levels in sera and nasal secretions were tested at 2-week intervals after each vaccination to assess the gpI immune response (Gao et al., 1994). mmunization of calves with this truncated gpI protein induced gpI-specific nasal IgA, IgG1, serum neutralizing antibodies and gpI-specific peripheral lymphocyte proliferation. All immunized calves were protected from clinical disease after BHV-1 challenge. Further, nine of ten immunized calves had no intranasal viral shedding. One animal shed a minimal amount of virus following challenge, but produced no antibodies to other viral proteins as evidenced by immunoprecipitation of 35S-labelled viral proteins by sera from virus-challenged animals (Gao et al., 1994). All animals were challenged with 5 x 10^5 p.f.u, of virulent Cooper strain BHV-1 (ATCC VR864) by intranasal aerosolization. Nasal swabs were collected daily for 12 days and viral replication and shedding were detected by titration on MDBK cells. The animals were monitored for signs of disease (fever, nasal mucosal lesions, nasal discharge, conjunctivitis and depression) (Gao et al., 1994). Bovine herpesvirus 1 recombinant vector vaccine BHVl/BRSVG VO_0004316 Recombinant vector vaccine Research BHV1 [Ref2146:Schrijver et al., 1997] intranasal immunization intranasal immunization Recombinant vector construction Vector BHV1 expressed the G protein of bovine respiratory syncytial virus (Schrijver et al., 1997). VO_0000286 A gE-negative bovine herpesvirus 1 (BHV1) vector vaccine carrying a gene coding for the G protein of bovine respiratory syncytial virus (BRSV) (BHV1/BRSV-G) induced the same high degree of protection in calves against BRSV infection and BHV1 infection as a multivalent commercial vaccine (Schrijver et al., 1997). Bovine herpesvirus 1 recombinant vector vaccine rLaSota/gDF encoding gD VO_0004319 Recombinant vector vaccine Research recombinant Newcastle Disease Virus pLaSota carrying the full-length antigenomic cDNA of the lentogenic NDV (Newcastle disease virus ) vaccine strain LaSota [Ref2151:Khattar et al., 2010] combined IN and IT routes combined IN and IT routes Recombinant vector construction Vector pLaSota expressed a chimeric gD in which the ectodomain of gD was fused with the transmembrane domain and cytoplasmic tail of the NDV fusion F glycoprotein (Khattar et al., 2010). VO_0000286 Following challenge with BHV-1, calves immunized with the recombinant NDVs had lower titers and earlier clearance of challenge virus compared to the empty vector control. Partial protection was observed (Khattar et al., 2010). Bovine herpesvirus 1 recombinant vector vaccine rLaSota/gDFL encoding gD VO_0004318 Recombinant vector vaccine Research recombinant Newcastle Disease Virus pLaSota carrying the full-length antigenomic cDNA of the lentogenic NDV(Newcastle disease virus ) vaccine strain LaSota [Ref2151:Khattar et al., 2010] combined IN and IT routes combined IN and IT routes Recombinant vector construction Vector pLaSota expressed gD without any modification (Khattar et al., 2010). VO_0000286 Following challenge with BHV-1, calves immunized with the recombinant NDVs had lower titers and earlier clearance of challenge virus compared to the empty vector control, and reduced disease was observed with rLaSota/gDFL. Partial protection was observed (Khattar et al., 2010). Bovine herpesvirus 1 UL23 mutant vaccine B8-D53 VO_0002949 Live, attenuated vaccine Research intranasal immunization intranasal immunization Gene mutation This UL23 mutant is from Bovine herpesvirus 1 (Kit et al., 1985). A UL23 mutant is attenuated in calves (Kit et al., 1985). A UL23 mutant induced protection in calves from challenge with wild type BHV-1 (Kit et al., 1985). Bovine herpesvirus 1 US8 (gE) mutant vaccine VO_0002950 Live, attenuated vaccine Research intranasal immunization The bovine herpesvirus 1 (BHV1) strain Za is a conventionally attenuated strain with a 2.7 kb deletion that encompasses the complete coding region for glycoprotein E (gE) (Kaashoek et al., 1995). intranasal immunization Gene mutation The bovine herpesvirus 1 (BHV1) strain Za is a conventionally attenuated strain with a 2.7 kb deletion that encompasses the complete coding region for glycoprotein E (gE) (Kaashoek et al., 1995). Calves given the vaccine with the highest antigen concentration were ad.equately protected against challenge; clinical symptoms were virtually absent and challenge virus shedding was significantly reduced as compared with unvaccinated calves (Kaashoek et al., 1995) Bovine Rhinotracheitis Killed Virus Vaccine (USDA: 1105.20) Heska Corporation VO_0001561 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 45H5.20) Texas Vet Lab, Inc. VO_0001877 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 45H5.21) Texas Vet Lab, Inc. VO_0001878 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida-Salmonella Typhimurium Bacterin-Toxoid (USDA: 4109.20) Texas Vet Lab, Inc. VO_0001879 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis Modified Live Virus Vaccine (USDA: 1101.00) Wyeth, Colorado Serum Company, Heska Corporation VO_0001562 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis Modified Live Virus Vaccine (USDA: 1101.20) Boehringer Ingelheim Vetmedica, Inc., Heska Corporation, Texas Vet Lab, Inc., Merial, Inc., Novartis Animal Health US, Inc. VO_0001563 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis Modified Live Virus Vaccine (USDA: 1101.21) Pfizer, Inc. VO_0001564 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis Modified Live Virus Vaccine (USDA: 1101.22) Intervet Inc. VO_0001565 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis Modified Live Virus Vaccine (USDA: 1101.B0) SolidTech Animal Health, Inc. VO_0001566 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis Modified Live Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4019.00) Wyeth, Colorado Serum Company VO_0001880 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis Modified Live Virus Vaccine-Leptospira Grippotyphosa-Hardjo-Pomona Bacterin (USDA: 4059.00) Colorado Serum Company VO_0001881 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis Modified Live Virus Vaccine-Leptospira Hardjo-Pomona Bacterin (USDA: 4069.20) Wyeth VO_0001882 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis Modified Live Virus Vaccine-Leptospira Pomona Bacterin (USDA: 4089.00) Colorado Serum Company, Heska Corporation VO_0001883 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis Modified Live Virus Vaccine-Leptospira Pomona Bacterin (USDA: 4089.20) Boehringer Ingelheim Vetmedica, Inc., Heska Corporation, Merial, Inc. VO_0001884 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3 Killed Virus Vaccine-Mannheimia Haemolytica-Pasteurella Multocida Bacterin (USDA: 4265.00) Boehringer Ingelheim Vetmedica, Inc. VO_0001885 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3 Modified Live Virus Vaccine (USDA: 1121.00) Colorado Serum Company, Heska Corporation VO_0001886 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3 Modified Live Virus Vaccine (USDA: 1121.20) Boehringer Ingelheim Vetmedica, Inc., Heska Corporation, Merial, Inc., Novartis Animal Health US, Inc. VO_0001887 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3 Modified Live Virus Vaccine (USDA: 1121.21) Pfizer, Inc. VO_0001888 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3 Modified Live Virus Vaccine (USDA: 1121.24) Pfizer, Inc. VO_0001889 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3 Modified Live Virus Vaccine (USDA: 1121.31) Intervet Inc. VO_0001890 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3 Modified Live Virus Vaccine-Haemophilus Somnus Bacterin (USDA: 4139.20) Boehringer Ingelheim Vetmedica, Inc. VO_0001891 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3 Modified Live Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4129.00) Colorado Serum Company, Heska Corporation VO_0001892 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3 Modified Live Virus Vaccine-Leptospira Grippotyphosa-Hardjo-Pomona Bacterin (USDA: 4179.00) Colorado Serum Company VO_0001893 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3 Modified Live Virus Vaccine-Leptospira Pomona Bacterin (USDA: 4209.00) Colorado Serum Company, Heska Corporation VO_0001894 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 1071.00) Heska Corporation VO_0001895 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 1071.20) Wyeth, Pfizer, Inc., Heska Corporation, Texas Vet Lab, Inc. VO_0001896 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 1071.21) Pfizer, Inc. VO_0001897 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 1071.22) Pfizer, Inc. VO_0001898 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 1071.23) Pfizer, Inc. VO_0001899 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Respiratory Syncytial Virus Killed Virus Vaccine (USDA: 11B5.20) Merial, Inc. VO_0001900 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine (USDA: 1155.20) Heska Corporation, Novartis Animal Health US, Inc. VO_0001901 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine (USDA: 1155.30) Heska Corporation VO_0001902 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine-Campylobacter Fetus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4145.20) Novartis Animal Health US, Inc. VO_0001903 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine-Campylobacter Fetus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4145.21) Heska Corporation VO_0001904 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 43S5.20) Texas Vet Lab, Inc. VO_0001905 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida-Salmonella Typhimurium Bacterin-Toxoid (USDA: 43T5.20) Texas Vet Lab, Inc. VO_0001906 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4335.20) Heska Corporation VO_0001907 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4336.30) Heska Corporation VO_0001908 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Modified Live Virus Vaccine (USDA: 1151.00) Colorado Serum Company, Heska Corporation VO_0001909 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Modified Live Virus Vaccine (USDA: 1151.20) Wyeth, Boehringer Ingelheim Vetmedica, Inc., Intervet Inc., Heska Corporation, Merial, Inc., Novartis Animal Health US, Inc. VO_0001910 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Modified Live Virus Vaccine (USDA: 1151.22) Boehringer Ingelheim Vetmedica, Inc., Intervet Inc. VO_0001911 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Modified Live Virus Vaccine (USDA: 1151.23) Boehringer Ingelheim Vetmedica, Inc. VO_0001912 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Modified Live Virus Vaccine (USDA: 1151.26) Pfizer, Inc. VO_0001913 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Modified Live Virus Vaccine-Campylobacter Fetus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4141.20) Boehringer Ingelheim Vetmedica, Inc., Intervet Inc. VO_0001914 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Modified Live Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4339.00) Colorado Serum Company, Heska Corporation VO_0001915 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Modified Live Virus Vaccine-Leptospira Grippotyphosa-Hardjo-Pomona Bacterin (USDA: 4359.00) Colorado Serum Company VO_0001916 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Modified Live Virus Vaccine-Leptospira Hardjo-Pomona Bacterin (USDA: 4399.20) Wyeth VO_0001917 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Modified Live Virus Vaccine-Leptospira Pomona Bacterin (USDA: 4389.00) Colorado Serum Company, Heska Corporation VO_0001918 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Modified Live Virus Vaccine-Leptospira Pomona Bacterin (USDA: 4389.20) Boehringer Ingelheim Vetmedica, Inc., Merial, Inc. VO_0001919 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Modified Live Virus Vaccine-Leptospira Pomona Bacterin (USDA: 4389.21) Heska Corporation VO_0001920 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Killed Virus Vaccine (USDA: 1175.20) Novartis Animal Health US, Inc. VO_0001921 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Killed Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4435.20) Novartis Animal Health US, Inc. VO_0001922 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Modified Live Virus Vaccine (USDA: 1171.00) Wyeth, Colorado Serum Company, Heska Corporation VO_0001923 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Modified Live Virus Vaccine (USDA: 1171.20) Heska Corporation, Merial, Inc. VO_0001924 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Modified Live Virus Vaccine (USDA: 1171.22) Intervet Inc., Pfizer, Inc. VO_0001925 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Modified Live Virus Vaccine (USDA: 1171.24) Pfizer, Inc. VO_0001926 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Modified Live Virus Vaccine (USDA: 1171.25) Pfizer, Inc. VO_0001927 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Modified Live Virus Vaccine (USDA: 1171.26) Pfizer, Inc. VO_0001928 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Modified Live Virus Vaccine (USDA: 1171.27) Pfizer, Inc. VO_0001929 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Modified Live Virus Vaccine (USDA: 1171.30) Boehringer Ingelheim Vetmedica, Inc. VO_0001930 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Modified Live Virus Vaccine-Campylobacter Fetus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4560.24) Pfizer, Inc. VO_0001931 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Modified Live Virus Vaccine-Campylobacter Fetus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4560.25) Pfizer, Inc. VO_0001932 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Modified Live Virus Vaccine-Haemophilus Somnus-Campylobacter Fetus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4489.30) Boehringer Ingelheim Vetmedica, Inc. VO_0001933 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Modified Live Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4439.00) Colorado Serum Company VO_0001934 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Modified Live Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4439.20) Wyeth, Heska Corporation, Merial, Inc. VO_0001935 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Modified Live Virus Vaccine-Leptospira Grippotyphosa-Hardjo-Pomona Bacterin (USDA: 4479.00) Colorado Serum Company VO_0001936 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3 Modified Live Virus Vaccine-Leptospira Pomona Bacterin (USDA: 4509.00) Colorado Serum Company, Heska Corporation VO_0001937 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Mannheimia Haemolytica Bacterin (USDA: 44A5.20) Wyeth VO_0001938 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 1181.00) Wyeth VO_0001939 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 1181.20) Boehringer Ingelheim Vetmedica, Inc., Intervet Inc., Heska Corporation, Novartis Animal Health US, Inc. VO_0001940 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 1181.21) Boehringer Ingelheim Vetmedica, Inc., Pfizer, Inc. VO_0001941 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 1181.22) Wyeth, Intervet Inc., Pfizer, Inc. VO_0001942 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 1181.23) Boehringer Ingelheim Vetmedica, Inc., Pfizer, Inc. VO_0001943 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 1181.24) Boehringer Ingelheim Vetmedica, Inc. VO_0001944 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 1181.25) Pfizer, Inc. VO_0001945 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 1181.26) Boehringer Ingelheim Vetmedica, Inc., Pfizer, Inc. VO_0001946 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 1181.28) Pfizer, Inc. VO_0001947 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 1181.30) Intervet Inc., Heska Corporation VO_0001948 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine (USDA: 1185.20) Boehringer Ingelheim Vetmedica, Inc., Merial, Inc., Novartis Animal Health US, Inc. VO_0001949 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine (USDA: 1185.22) Wyeth VO_0001950 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Campylobacter Fetus-Haemophilus Somnus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44M5.20) Novartis Animal Health US, Inc. VO_0001951 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Campylobacter Fetus-Haemophilus Somnus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44M5.22) Novartis Animal Health US, Inc. VO_0001952 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Campylobacter Fetus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44B5.20) Novartis Animal Health US, Inc. VO_0001953 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Campylobacter Fetus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44B6.20) Novartis Animal Health US, Inc. VO_0001954 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Campylobacter Fetus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44B6.21) Novartis Animal Health US, Inc. VO_0001955 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Haemophilus Somnus Bacterin (USDA: 44C5.20) Novartis Animal Health US, Inc. VO_0001956 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Haemophilus Somnus Bacterin (USDA: 44C7.20) Wyeth VO_0001957 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Haemophilus Somnus Bacterin (USDA: 44D5.20) Boehringer Ingelheim Vetmedica, Inc. VO_0001958 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Haemophilus Somnus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44D7.20) Wyeth, Boehringer Ingelheim Vetmedica, Inc., Novartis Animal Health US, Inc. VO_0001959 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Haemophilus Somnus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44D7.22) Novartis Animal Health US, Inc. VO_0001960 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica Bacterin (USDA: 4477.20) Wyeth VO_0001961 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4465.20) Wyeth, Boehringer Ingelheim Vetmedica, Inc., Merial, Inc., Novartis Animal Health US, Inc. VO_0001962 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4465.21) Wyeth VO_0001963 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4465.22) Wyeth VO_0001964 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4466.20) Novartis Animal Health US, Inc. VO_0001965 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4466.21) Novartis Animal Health US, Inc. VO_0001966 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Killed Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona-Mannheimia Haemolytica Bacterin (USDA: 4475.20) Wyeth VO_0001967 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live & Killed Virus Vaccine (USDA: 1187.20) Heska Corporation VO_0001968 Live, attenuated vaccine; Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live & Killed Virus Vaccine (USDA: 1187.21) Wyeth, Pfizer, Inc., Merial, Inc. VO_0001969 Live, attenuated vaccine; Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live & Killed Virus Vaccine (USDA: 1187.22) Merial, Inc. VO_0001970 Live, attenuated vaccine; Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live & Killed Virus Vaccine (USDA: 1187.25) Pfizer, Inc. VO_0001971 Live, attenuated vaccine; Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live & Killed Virus Vaccine (USDA: 1189.00) Heska Corporation VO_0001972 Live, attenuated vaccine; Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live & Killed Virus Vaccine (USDA: 1189.21) Merial, Inc. VO_0001973 Live, attenuated vaccine; Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live & Killed Virus Vaccine (USDA: 1189.30) Heska Corporation VO_0001974 Live, attenuated vaccine; Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live & Killed Virus Vaccine-Campylobacter Fetus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44B9.21) Heska Corporation VO_0001975 Live, attenuated vaccine; Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live & Killed Virus Vaccine-Campylobacter Fetus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44B9.22) Pfizer, Inc. VO_0001976 Live, attenuated vaccine; Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live & Killed Virus Vaccine-Haemophilus Somnus Bacterin (USDA: 44F9.20) Boehringer Ingelheim Vetmedica, Inc. VO_0001977 Live, attenuated vaccine; Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live & Killed Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4469.20) Heska Corporation VO_0001978 Live, attenuated vaccine; Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live & Killed Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4469.22) Pfizer, Inc. VO_0001979 Live, attenuated vaccine; Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live & Killed Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4469.25) Pfizer, Inc. VO_0001980 Live, attenuated vaccine; Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live & Killed Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4469.30) Heska Corporation VO_0001981 Live, attenuated vaccine; Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Campylobacter Fetus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44B1.20) Boehringer Ingelheim Vetmedica, Inc., Intervet Inc. VO_0001982 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Campylobacter Fetus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44B1.22) Pfizer, Inc. VO_0001983 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Campylobacter Fetus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44B1.23) Pfizer, Inc. VO_0001984 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Campylobacter Fetus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44B1.25) Pfizer, Inc. VO_0001985 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Haemophilus Somnus Bacterin (USDA: 44C9.20) Boehringer Ingelheim Vetmedica, Inc., Pfizer, Inc. VO_0001986 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Haemophilus Somnus Bacterin (USDA: 44C9.21) Boehringer Ingelheim Vetmedica, Inc. VO_0001987 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Haemophilus Somnus Bacterin (USDA: 44C9.23) Boehringer Ingelheim Vetmedica, Inc. VO_0001988 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Haemophilus Somnus Bacterin (USDA: 44C9.24) Boehringer Ingelheim Vetmedica, Inc. VO_0001989 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Haemophilus Somnus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44D9.20) Boehringer Ingelheim Vetmedica, Inc. VO_0001990 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Haemophilus Somnus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44D9.21) Boehringer Ingelheim Vetmedica, Inc. VO_0001991 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Haemophilus Somnus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44D9.23) Boehringer Ingelheim Vetmedica, Inc. VO_0001992 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Haemophilus Somnus-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 44D9.24) Boehringer Ingelheim Vetmedica, Inc. VO_0001993 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4461.20) Wyeth, Boehringer Ingelheim Vetmedica, Inc., Intervet Inc., Heska Corporation VO_0001994 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4461.21) Pfizer, Inc. VO_0001995 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4461.22) Wyeth, Intervet Inc. VO_0001996 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4461.25) Pfizer, Inc. VO_0001997 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4469.00) Heska Corporation VO_0001998 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4469.01) Boehringer Ingelheim Vetmedica, Inc. VO_0001999 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4469.23) Boehringer Ingelheim Vetmedica, Inc. VO_0002000 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4469.24) Boehringer Ingelheim Vetmedica, Inc., Pfizer, Inc. VO_0002001 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Leptospira Canicola-Grippotyphosa-Hardjo-Icterohaemorrhagiae-Pomona Bacterin (USDA: 4469.26) Pfizer, Inc. VO_0002002 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Leptospira Hardjo Bacterin (USDA: 4L49.20) Pfizer, Inc. VO_0002003 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Mannheimia Haemolytica Toxoid (USDA: 4X49.20) Wyeth VO_0002004 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Mannheimia Haemolytica Toxoid (USDA: 4X49.21) Wyeth VO_0002005 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 45B9.20) Boehringer Ingelheim Vetmedica, Inc. VO_0002006 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 45B9.21) Boehringer Ingelheim Vetmedica, Inc. VO_0002007 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus-Mannheimia Haemolytica-Pasteurella Multocida Modified Live Virus, Avirulent Live Culture Vaccine (USDA: 11A8.22) Intervet Inc. VO_0002008 Live vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Respiratory Syncytial Virus Killed Virus Vaccine (USDA: 11A5.20) Merial, Inc. VO_0002009 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 11A1.20) Heska Corporation VO_0002010 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 11A1.26) Pfizer, Inc. VO_0002011 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Respiratory Syncytial Virus Modified Live Virus Vaccine (USDA: 11A1.30) Heska Corporation VO_0002012 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Respiratory Syncytial Virus Modified Live Virus Vaccine-Leptospira Pomona Bacterin (USDA: 4367.20) Heska Corporation VO_0002013 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Respiratory Syncytial Virus Modified Live Virus Vaccine-Leptospira Pomona Bacterin (USDA: 4367.26) Pfizer, Inc. VO_0002014 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Respiratory Syncytial Virus Modified Live Virus Vaccine-Leptospira Pomona Bacterin (USDA: 4367.30) Heska Corporation VO_0002015 Live, attenuated vaccine Licensed USA G from BRSV Bovine orthopneumovirus 1489813 9631274 BovRSVgp07 AF092942 NP_048054 11246 4689 5528 + mRNA 10.63 27981.73 257 >NC_001989.1:4689-5528 Bovine respiratory syncytial virus, complete genome TGGGGCAAATACAAGTATGTCCAACCATACCCACCATCTTAAATTCAAGACATTAAAGAGGGCTTGGAAA GCCTCAAAATACTTCATAGTAGGATTATCATGTTTATATAAGTTCAATTTAAAATCCCTTGTCCAAACGG CTTTGACCACCTTAGCAATGATAACCTTGACATCACTCGTCATAACAGCCATTATTTACATTAGTGTGGG AAATGCTAAAGCCAAGCCCACATCCAAACCAACCATCCAACAAACACAACAGCCCCAAAACCATACCTCA CCATTTTTCACAGAGCACAACTACAAATCAACTCACACATCAATTCAAAGCACCACACTGTCCCAACTAC CAAACACAGACACCACTAGAGAAACTACATACAGTCACTCAATCAACGAAACCCAAAACAGAAAAATCAA AAGCCAATCCACTCTACCCGCCACCAGAAAACCACCAATTAACCCATCGGGAAGCAACCCCCCTGAAAAC CACCAAGACCACAACAACTCCCAAACACTCCCCTATGTGCCTTGCAGTACATGTGAAGGTAATCTTGCTT GTTTATCACTCTGCCAAATCGGGCCGGAGAGAGCACCAAGCAGAGCCCCTACAATCACCCTCAAAAAGAC TCCAAAACCCAAAACCACCAAAAAGCCAACCAAGACAACAATCCACCACAGAACCAGCCCTGAAGCCAAA CTGCAACCCAAAAACAACACGGCAGCTCCACAACAAGGCATCCTCTCTTCACCAGAACACCACACAAATC AATCAACTACACAGATCTAACAACACACCTCCATATAATATCAATTATGTTCATATATAGTTATTTAAAA >NP_048054.1 G [Bovine orthopneumovirus] MSNHTHHLKFKTLKRAWKASKYFIVGLSCLYKFNLKSLVQTALTTLAMITLTSLVITAIIYISVGNAKAK PTSKPTIQQTQQPQNHTSPFFTEHNYKSTHTSIQSTTLSQLPNTDTTRETTYSHSINETQNRKIKSQSTL PATRKPPINPSGSNPPENHQDHNNSQTLPYVPCSTCEGNLACLSLCQIGPERAPSRAPTITLKKTPKPKT TKKPTKTTIHHRTSPEAKLQPKNNTAAPQQGILSSPEHHTNQSTTQI Protective antigen gB Bovine herpesvirus 1 349734084 CDD:223014 10320 ? glycoprotein B 6.96 16194.78 216 glycoprotein BALF4; Provisional >AEQ16470.1 glycoprotein B, partial [Bovine alphaherpesvirus 1] SKAEYLRSGRKVVAFDRDDDPWEAPLKPARLSAPGVRGWHTTDDVYTALGSAGLYRTGTSVNCIVEEVEA RSVYPYDSFALSTGDIIYMSPFYGLREGAHREHTSYSPERFQQIEGYYKRDMATGRRLKEPVSRNFLRTQ HVTVAWDW Protective antigen gIV Bovine herpesvirus 1 330746 CDD:223029 10320 ? glycoprotein gIV 4.96 42868.83 481 envelope glycoprotein D; Provisional >AAA46050.1 glycoprotein gIV [Bovine alphaherpesvirus 1] MQGPTLAVLGALLAVAVSLPTPAPRVTVYVDPPAYPMPRYNYTERWHTTGPIPSPFADGREQPVEVRYAT SAAACDMLALIADPQVGRTLWEAVRRHARAYNATVIWYKIESGCARPLYYMEYTECEPRKHFGYCRYRTP PFWDSFLAGFAYPTDDELGLIMAAPARLVEGQYRRALYIDGTVAYTDFMVSLPAGDCWFSKLGAARGYTF GACFPARDYEQKKVLRLTYLTQYYPQEAHKAIVDYWFMRHGGVVPPYFEESKGYEPPPAADGGSPAPPGD DEAREDEGETEDGAAGREGNGGPPGPEGDGESQTPEANGGAEGEPKPGPSPDADRPEGWPSLEAITHPPP APATPAAPDAVPVSVGIGIAAAAIACVAAAAAGAYFVYTRRRGAGPLPRKPKKLPAFGNVNYSALPG Protective antigen UL23 Bovine herpesvirus 1 1487380 9629852 BoHV1_gp21 Z78205 NP_045336 10320 63259 67035 + thymidine kinase 6.75 35103.27 359 2.7.1.21 >gi|9629818:63259-67035 Bovine herpesvirus 1, complete genome CATGGCCGAGCCCGCGCGCGCTCTCCGCGTCGTGCGTATCTACCTGGACGGCGCGCACGGGCTGGGAAAG ACAACAACGGGCCGCGCGCTCGCGGCCGCTTCCACCGCTGGGGAGGGCGTGCTCTTTTTCCCGGAGCCGA TGGCGTACTGGCGCACGATGTTTGGTACGGACGCCTTAAGTGGGATCCTCGCGGCGTCTGCGCGATGCGC CGCAGCCTCGCACGGGAGCGCACGCGGCGCCGGCGGGCCGGCGCACCGCGCAGACGCGGACGCGGCGGGC CTGGTTGCGTACTACCAGGCCAGGTTCGCGGCCCCGTACTTAATTTTGCACGCGCGCGTGTCCGCGCTGC TGGCGCCGCCTGGGCCGGCGCCGGGCGGCACTGTGACCCTCGTGTTCGACCGCCACCCCGTGGCCGCGTG CCTCTGCTACCCCTTCGCCCGCTACTGCCTCCGCGAGATCAACGCGGAAGATCTGCTCATGCTCGCGGCC GCCATGCCCCCGGAAGCGCCCGGGGCCAACCTCGTCGTGTGCACCCTCCCCCCGGCCGAGCAACAGCGCC GCCTGGCGGCGCGGGCCAGGCCCGGAGACCGCGCGGACGCGGGCTTTCTGGTCGCTGTGCGCAATGCTTA CGCGCTCCTGGTGAACACGTGCGCTTTCCTGCGCGCGGGGGGCGCATGGCGCGACGGCTGGGACGCGCTG GAGTGGGCGGACGCAAATGCATTGGCCGCGCTCGCAGACCCCAGTTGTGATGAATGCAAAATGGCGCCGG CGCCGGCGCTGCGCGACACCCTGTTCGCGGCGCTCAAGTGCCGCGAGCTCTACCCGGGCGGCGGGACGGG CTTGCCCGCGGTTCACGCCTGGGCGCTGGACGCCCTGGCCGGCCGCCTCGCCGCCCTCGAGGTGTTCGTG CTGGACGTGTCCGCGGCGCCAGACGCGTGCGCGGCCGCCGTACTGGACATGCGGCCCGCCATGCAGGCCG CTTGCGCGGACGGGGCGGCGGGCGCGACGCTGGCGACCCTGGCGCGTCAGTTCGCGCTAGAGATGGCGGG GGAGGCCACGGCGGGCCCTAGGGGACTATAAAGCTGCCCCTGCGCTCGCTCGCTCGCTGCATTTGCGCCC CGATCGCCTTACGGGGACTCGGCGCTCGGCGGATCCCCTCCCGGCCCCGCCGCGAAGCGAGCCGCCAGAC AAAAAAATGCGGCGCCCGCTCTGCGCGGCGCTATTGGCAGCGGCTGTCCTCGCGCTCGCCGCGGGCGCCC CCGCCGCCGCCCGCGGCGGCGCGGGGGGCCGAAGCAGGGAGCACAGAGACGCCCGATACGAAATCGAAGA GTGGGAAATGGTGGTCGGAGCCGGGCCGGCCGTGCACACGTTCACCATCCGCTGCCTCGGGCCGCGGGGC ATTGAGCGCGTGGCCCACATTGCAAACCTCAGCCGGCTGCTGGACGGGTACATAGCGGTCCACGTTGACG TTGCGCGCACCTCTGGCCTGCGGGACACCATGTTTTTCCTGCCGCGCGCGGCCGTCGACAACGCTTCGGC CGCTGACATTCCGGACACCCCGGCCGTACAGTCGCACCCGGGGCTCTTCGGGGCGGCCTTTTCCTGGAGC TACTTGCAAACGCGCCACCTCGTAGACTACGACCTGGTGCCGAGCCGCCCCCTGCAGGACTGGTACTTTT CGCAGGCGCGCGCCGAGAGCAACGCCGCGCGCCCGCCGCCCGCCCCGCGCGTCACACCAACGCCGGCGGG GCGGGTGGCCGCTTTTGACATCAACGACGTGCTGGCCAGCGGCCCGGAGCACTTCTTTGTGCCTGTGCGA GCGGACCGCAAGCGGCGCGAGCGCCACGTGGCGGATTTTGCCGCGGTGTGGCCCGTGTCCTACATCCCCG CAGGACGGGCAGTGCTAAGCTGCGAGCGAGCCGCGGCTCGGCTGGCGGTGGGGCTCGGCTTCCTGAGCGT CTCGGTGACGTCGCGGGACCTCCTGCCTCTGGAGTTTATGGTCGCGCCCGCGGACGCCAACGTGCGCATG ATTACCGCCTTTAACGGGGGCGGCGCTTTTCCGCCACCCGGGCCCGCGGCCGGTCCGCAGCGGCGGGCCT ACGTAATCGGCTACGGGAACTCGCGGCTGGACAGCCATATGTATCTGACCATGCGCGAGGTGGCGTCGTA CGCGAACGAGCCCGCTGACTTTCGCGCGCACTTGACCGCCGCGCACCGGGAGGCTTTTTTGATGCTCCGG GAGGCGGCAGCCGCGCGCCGCGGACCGAGCGCCGGCCCCGCGCCCAACGCTGCCTACCACGCGTACCGGG TCGCGGCGCGGCTGGGACTCGCGCTCTCCGCGCTCACCGAGGGGGCGCTCGCGGACGGCTACGTGCTCGC GGAGGAGCTAGTCGACCTTGACTACCACCTCAAGCTGCTGTCGCGCGTGCTGCTCGGCGCAGGGCTTGGC TGCGCCGCCAACGGCCGCGTGCGCGCCCGCACCATCGCGCAGCTGGCCGTGCCCCGCGAGCTACGCCCGG ACGCGTTCATCCCAGAGCCCGCCGGGGCGGCGCTCGAGAGCGTGGTGGCCCGCGGGCGCAAGCTGCGCGC CGTGTACGCTTTTTCGGGTCCGGACGCTCCGCTAGCTGCGCGGCTGCTGGCCCACGGCGTGGTGTCGGAC CTCTACGACGCCTTCCTGCGCGGCGAGCTGACGTGGGGGCCGCCCATGCGCCACGCGCTTTTTTTCGCCG TCGCGGCGTCGGCGTTCCCCGCGGACGCCCAGGCGCTGGAGCTCGCGCGGGACGTGACGCGCAAGTGCAC GGCTATGTGCACCGCCGGGCACGCCACGGCGGCCGCGCTGGACCTGGAGGAGGTATACGCGCACGTCGGC GGCGGCGCCGGGGGCGACGCGGGCTTTGAGCTGCTGGATGCCTTCTCGCCGTGCATGGCCTCTTTCCGCC TGGACTTGCTCGAGGAGGCGCACGTGCTGGACGTGCTCTCGGCCGTGCCCGCGCGGGCCGCGCTGGACGC CTGGCTGGAGGCGCAGCCCGCGGCCGCCGCGCCGAACCTCAGCGCGGCGGCGCTCGGCATGCTGGGCCGG GGAGGCCTCTTCGGCCCGGCGCACGCCGCCGCGCTCGCGCCCGAGCTCTTCGCGGCGCCCTGCGGCGGGT GGGGCGCGGGCGCCGCCGTGGCGATCGTCCCCGTGGCGCCGAACGCTAGCTATGTCATCACGCGCGCACA TCCGCGGCGCGGGCTGACGTACACCCTCCAGGGAATTGACGTCGCTAACCCCCTGCTGGTGACTTTTGTG CGCGGCACGTCGTGCGTGTCGGCCAGCGGCGCGGTGGAGGCGCGCCGCCTTGCGGTCCCCGGCCCGCTGG ACGCGTGCGCCTACTGCGGCAGCGTGTTCGTGCGATACTTGCCCTCGGGCGCGGTTATGGACATCGTGCT CATTGCGGACAAGCGCACCGAGGTTGAGTTCTCGCGCGGCGCCAACTCTAGCATGCCCGTCTTCAACCCT CGCCTCCACAGCGGGCGCTCCCGCGCCATGCTGCTGTTCCCCAACGGGACGGTCGTAAGCGTCCTGGCCT TCGCCGGGCACGAAGCGCCGACGTTTTCGCCGGCGTACGTCTGGGCGTCGGTAGGCGGGGCGCTGGTCGC GGGTACCACGATATACGCCATCGCGAAGATGCTGTGCAGCTCGGTCCCGCTCGCGCGCGGGTACTCGTCG GTCCCGGTGTTTTAGCGCGCGCGTTGCCCTCCCCCCCTTCCCCCTTTTCACGTCTCCCCGCCAATAA >gi|9629852|ref|NP_045336.1| thymidine kinase [Bovine herpesvirus 1] MAEPARALRVVRIYLDGAHGLGKTTTGRALAAASTAGEGVLFFPEPMAYWRTMFGTDALSGILAASARCA AASHGSARGAGGPAHRADADAAGLVAYYQARFAAPYLILHARVSALLAPPGPAPGGTVTLVFDRHPVAAC LCYPFARYCLREINAEDLLMLAAAMPPEAPGANLVVCTLPPAEQQRRLAARARPGDRADAGFLVAVRNAY ALLVNTCAFLRAGGAWRDGWDALEWADANALAALADPSCDECKMAPAPALRDTLFAALKCRELYPGGGTG LPAVHAWALDALAGRLAALEVFVLDVSAAPDACAAAVLDMRPAMQAACADGAAGATLATLARQFALEMAG EATAGPRGL Virmugen A thymidine kinase-deficient mutant (B8-D53) showed no detectable thymidine kinase-inducing activity and was attenuated in calves. B8-D53 also induced protection from challenge with wild type BHV-1 [Ref1984:Kit et al., 1985]. UL44 Bovine alphaherpesvirus 1 VO_0011043 4783424 9629830 BoHV1_gp105 KC756965 NP_045314 10320 16612 18208 - envelope glycoprotein C 8.25 50150.91 508 envelope glycoprotein C; the Herpesviridae are non-segmented dsDNA viruses with genomes ranging from 120-230kbp; although herpes viruses vary greatly in sequence identity and homology, they all share four common elements: an envelope, a tegument which is composed of viral enzymes, a capsid of 162 capsomers, and a core composed of genomic DNA;virion envelope glycoproteins bind to cellular receptors; the nonessential glycoprotein gC interacts with cell surface proteoglycans, whereas the essential glycoprotein gD is involved in stable secondary attachment >NC_001847.1:16612-18208 Bovine herpesvirus 1, complete genome GTTTATTATGAAATTGTACATGCGTGGTCTTTGGGGGGGGGCGCGGCGGCTTTGCCGTCGGGGCCCCGCG CCTACAGGCGCGCCCGGGCCTTGCGCCGCAGGCACGAGGCCGCCACCAGCAGGAGCCCCACGGCGCCGAG ACCGCAGGCGACGGCCACGACGCTGACGAGGACGGGGCTTCCGATTAGGCCGGGCGAGGCGTCGTACGTG GCGGTCGCGGAGAACTCGGGCAGCGGTGCCGGGTAGCCAGTGGCGGTGCAGGTGTAGTCGACGGGCCCGT CGGTTGTAGAAAGCAGGCGCACGCCGCGCAGGTTTACCAGCCCGGGCCGCTCGGCGCAGACGCCGGTCTG CTCAGTGCGCGACGGGGCGATGCCGTCGCGCACCGTCCAGCGCAGGGAGACGCGCCCCTCGGGGACGCAG CGCGCCTCGCAGACGGCCTCGCCGCCCTCGAAGTACACGCGCAGCTCGGGCGGGCGGTAAACGGCCGGCG TGCCAGCCGCGTAAAAGCGGCGCTCCATGTTAGCGCTCTGGAACCAGGAGACGTCGCAGCGCAGGTTGGG CGGGTGGGCGGTTGGCGTCGCGTCCTCGAGCGTAAGGACGGACGTGCGCGAAAAGAGCCCGGAGTCGTCG ACCGTAAAGACGTCGCGCGCGTGCCGAGCCTCCACGGGGTAGCCGTTGCGGAACCAGTGCAGGCGCGTGG AGCGCGGCGGGTAGTACTCGGCGGCGCGGCACACGGCCGCGTAGCCGGCGCCTTCTAGCGCTGGCTGGGG TTCGACGGAAACAGCGGGCGCGCGGTGCGTCGTGACGGTCACGACCTTGCGCTGTGACTTGGTGCCCATG TCGCGGCGCCAAGTGTACACGCCCTCGGTGGCGGCCGTCAGGGAGCGCACGGTCAGGGGCAAGTTGCGGG GGTCGGCGGGCGAAGGGAAAATATAGTTGTCGCCCAGCTCCGCGCTACGGTACGCGATCGAGCCGTTCTG GGCTACGAACAGCAGCACGGGCGGGGCCCGCGGAAAGGGGTTGCGCACGGCCTCGTCGTCGCCGCGCGTG GAGCGGAACCTGCCCACGCGCTGAAACCAGAGCTCCAGGCGCGCACCGCCAGTGGCGTTGTCGGCCACGC CGCAGTGCACGTACAGCGGCTCGGCGTACGAGGCGGCCACGGCCTCGCGCTCGCAGAGCATCCACTTGCG CTCCTTCGGCGGGGCTTTGCTCGGCCGCGGGGGGCGAGGCCGCCCCCCGCCGCTAGGTCGCCCATCGCGG CTCGCGTTGCCAGCGCCGCCGGGTCGCCCGTCCTCGGGCGGGGCGGGCGGCGGCGTGCTGTTGGTAACGG GCGGGTCGTGCTCGGACACGGACGGCGGCTCCGGCGTCCCCACGGGCGTAGTAGCACCGGGCGTGCTGTC CTCTGGCGTAGCGTCGGGGCTGTTGGGCGTGGGGGGCGTTGCGCCGGTGGTCCCAGCGGAGCTTTCCGTC TCGGTTGGGGACGGGGAGGGCGGAGGCGAGGGCGAGGCTTCCGCCTCCTCGGCGAGCCCCCGCCGGGCAG ACAGGAGCGCCCAGGCGAAAATAGCTGCGATCAGCCACGCTCGCCCCAGCGGGCCCA >NP_045314.1 envelope glycoprotein C [Bovine alphaherpesvirus 1] MGPLGRAWLIAAIFAWALLSARRGLAEEAEASPSPPPSPSPTETESSAGTTGATPPTPNSPDATPEDSTP GATTPVGTPEPPSVSEHDPPVTNSTPPPAPPEDGRPGGAGNASRDGRPSGGGRPRPPRPSKAPPKERKWM LCEREAVAASYAEPLYVHCGVADNATGGARLELWFQRVGRFRSTRGDDEAVRNPFPRAPPVLLFVAQNGS IAYRSAELGDNYIFPSPADPRNLPLTVRSLTAATEGVYTWRRDMGTKSQRKVVTVTTHRAPAVSVEPQPA LEGAGYAAVCRAAEYYPPRSTRLHWFRNGYPVEARHARDVFTVDDSGLFSRTSVLTLEDATPTAHPPNLR CDVSWFQSANMERRFYAAGTPAVYRPPELRVYFEGGEAVCEARCVPEGRVSLRWTVRDGIAPSRTEQTGV CAERPGLVNLRGVRLLSTTDGPVDYTCTATGYPAPLPEFSATATYDASPGLIGSPVLVSVVAVACGLGAV GLLLVAASCLRRKARARL Protective antigen A DNA vaccine expressing glycoprotein C (gC, UL44) of bovine herpesvirus-1 (BHV-1) was evaluated for inducing immunity in bovines. Results indicate that DNA immunization with gC could induce neutralizing antibody and lymphoproliferative responses with BHV-1 responsive memory B cells in bovines. However, the immunity developed sufficient for only partial protection against BHV-1 challenge [Ref1032:Gupta et al., 2001]. UL49 Bovine alphaherpesvirus 1 VO_0011040 1487397 9629826 BoHV1_gp05 GU591892 NP_045310 10320 9383 10228 + tegument protein VP22 10.07 25574.19 258 >NC_001847.1:9383-10228 Bovine herpesvirus 1, complete genome CATGGCCCGGTTCCACAGGCCCTCCGAAGACGAGGACGATTACGAGTACAGCGACCTTTGGGTGCGAGAA AACAGCCTCTATGACTACGAGTCCGGCTCGGATGACCACGTATACGAAGAGCTGCGCGCCGCGACGAGCG GACCCGAGCCGAGCGGGCGGCGCGCTAGCGTCCGTGCGTGCGCCAGCGCTGCAGCCGTCCAGCCCGCCGC CCGCGGCCGCGATCGAGCCGCAGCCGCGGGGACGACCGTAGCTGCGCCCGCCGCCGCGCCGGCCCGCCGC TCGAGCAGCCGGGCGTCCTCGCGCCCGCCGCGAGCTGCCGCCGACCCGCCCGTCCTCCGGCCAGCCACGC GCGGGTCCTCCGGCGGCGCCGGGGCAGTCGCCGTCGGTCCACCTCGACCTCGCGCGCCCCCCGGTGCTAA TGCTGTTGCGTCTGGCCGGCCGCTGGCGTTCAGCGCGGCTCCGAAAACGCCCAAGGCGCCCTGGTGTGGA CCGACGCACGCCTACAACCGAACGATCTTTTGCGAGGCCGTCGCGCTCGTGGCCGCCGAGTACGCCCGGC AGGCGGCTGCCAGCGTCTGGGACTCGGACCCCCCAAAGAGCAACGAGCGATTGGATCGCATGTTGAAGTC GGCGGCAATTCGCATCCTCGTGTGCGAGGGCTCCGGGCTTCTCGCCGCCGCGAACGACATCTTGGCCGCG CGGGCCCAGCGCCCCGCCGCGCGCGGGAGCACAAGCGGCGGGGAAAGCCGCCTTCGCGGCGAGCGGGCCC GGCCGTAGCGCGAGCGGGAGGGCTTTTTCGACGCGCGCGGCTTAAGCAGCGCGCTGCTGTGCTAGTATGA AAATAA >NP_045310.1 tegument protein VP22 [Bovine alphaherpesvirus 1] MARFHRPSEDEDDYEYSDLWVRENSLYDYESGSDDHVYEELRAATSGPEPSGRRASVRACASAAAVQPAA RGRDRAAAAGTTVAAPAAAPARRSSSRASSRPPRAAADPPVLRPATRGSSGGAGAVAVGPPRPRAPPGAN AVASGRPLAFSAAPKTPKAPWCGPTHAYNRTIFCEAVALVAAEYARQAAASVWDSDPPKSNERLDRMLKS AAIRILVCEGSGLLAAANDILAARAQRPAARGSTSGGESRLRGERARP Protective antigen Protection against a BHV-1 challenge was obtained in calves immunized with the plasmid encoding tgD-VP22 (UL49), as shown by significant reductions in viral excretion [Ref1028:Zheng et al., 2005]. US6 Bovine alphaherpesvirus 1 VO_0011041 1487406 9629886 BoHV1_gp41 KU198480 NP_045370 10320 118895 121435 + envelope glycoprotein D 4.96 42542.47 417 envelope glycoprotein D; the Herpesviridae are non-segmented dsDNA viruses with genomes ranging from 120-230kbp; although herpes viruses vary greatly in sequence identity and homology, they all share four common elements: an envelope, a tegument which is composed of viral enzymes, a capsid of 162 capsomers, and a core composed of genomic DNA;virion envelope glycoproteins bind to cellular receptors; the nonessential glycoprotein gC interacts with cell surface proteoglycans, whereas the essential glycoprotein gD is involved in stable secondary attachment >NC_001847.1:118895-121435 Bovine herpesvirus 1, complete genome CATGCAAGGGCCGACATTGGCCGTGCTGGGCGCGCTGCTCGCCGTTGCGGTGAGCTTGCCTACACCCGCG CCGCGGGTGACGGTATACGTCGACCCGCCGGCGTACCCGATGCCGCGATACAACTACACTGAACGCTGGC ACACTACCGGGCCCATACCGTCGCCCTTCGCAGACGGCCGCGAGCAGCCCGTCGAGGTGCGCTACGCGAC GAGCGCGGCGGCGTGCGACATGCTGGCGCTGATCGCAGACCCGCAGGTGGGGCGCACGCTGTGGGAAGCG GTACGCCGGCACGCGCGCGCGTACAACGCCACGGTCATATGGTACAAGATCGAGAGCGGGTGCGCCCGGC CGCTGTACTACATGGAGTACACCGAGTGCGAGCCCAGGAAGCACTTTGGGTACTGCCGCTACCGCACACC CCCGTTTTGGGACAGCTTCCTGGCGGGCTTCGCCTACCCCACGGACGACGAGCTGGGACTGATTATGGCG GCGCCCGCGCGGCTCGTCGAGGGCCAGTACCGACGCGCGCTGTACATCGACGGCACGGTCGCCTATACAG ATTTCATGGTTTCGCTGCCGGCCGGGGACTGCTGGTTCTCGAAACTCGGCGCGGCTCGCGGGTACACCTT TGGCGCGTGCTTCCCGGCCCGGGATTACGAGCAAAAGAAGGTTCTGCGCCTGACGTATCTCACGCAGTAC TACCCGCAGGAGGCACACAAGGCCATAGTCGACTACTGGTTCATGCGCCACGGGGGCGTCGTTCCGCCGT ATTTTGAGGAGTCGAAGGGCTACGAGCCGCCGCCTGCCGCCGATGGGGGTTCCCCCGCGCCACCCGGCGA CGACGAGGCCCGCGAGGATGAAGGGGAGACCGAGGACGGGGCAGCCGGGCGGGAGGGCAACGGCGGCCCC CCAGGACCCGAAGGCGACGGCGAGAGTCAGACCCCCGAAGCCAACGGAGGCGCCGAGGGCGAGCCGAAAC CCGGCCCCAGCCCCGACGCCGACCGCCCCGAAGGCTGGCCGAGCCTCGAAGCCATCACGCACCCCCCGCC CGCCCCCGCTACGCCCGCGGCCCCCGACGCCGTGCCGGTCAGCGTCGGGATCGGCATTGCGGCTGCGGCG ATCGCGTGCGTGGCCGCCGCCGCCGCCGGCGCGTACTTCGTCTATACGCGCCGGCGCGGTGCGGGTCCGC TGCCCAGAAAGCCAAAAAAGCTGCCGGCCTTTGGCAACGTCAACTACAGCGCGCTGCCCGGGTGAGCGGC CTAGGCCCTCCCCCGACCGCCCCCTTTGCTCCTAGCCCCGGCTCCTGCCGAGCCGCGCGGGGCGGGAGAT AAAGCGCCCGCGCGTCGGCGACTCAAGCCATTGCCGCGACCTTGTCCTCCGGCGCGCTCGCGATGCGGTG CCTGTTGCTCTGGATGGTGGTGCTGGCCGCGCGAGCGGCGCCCGCTCGCAGCCTTGTGTATCGCGGCGAG GCAGTCGGCCTGCGCGCGGACGGCCCCGTAGCGTTCGCTGTCCACCCGACCGACGCAACGCTCGCGCTGC GGGGCCGGCTGATTTTCCTGGAACACCAGCTCCCGGCCGGGCGGCGCTACAACGGGACCGTCGAGCTGCT GCGCTACCACGCCGCGGGCGACTGCTTCGTTATGCTGCAGACGACCGCGTTCGCCTCCTGCCCGCGCGTC GCGAACGACGCCTTTCGCTCCTGCCTGCACGCCGACACGCGCCCCGCTCGCAGCGAGCGGCGCGCGAGCG CCGCGGTCGAAAACCACGTGCTCTTCTCCATCGCCCATCCGCGCCCAATAGACTCAGGGCTCTACTTTCT GCGCGTCGGCATCTACGGCGGCACCGCGGGCAGCGAGCGCCGCCGAGACGTCTTTCCCTTGGCCGCGTTT GTACACAGCTTCGGTGAGCCCGGAGACCCAGAGGCCGCGGCCGCGCACCCCGGCGCCGTCGAGGCAGTCG CGACCCGCTGCGAGCGGGGCCTCGACGCCAGCTCGGCGAGCCTCTACGACCGCGCGCTGGCGGCGTTCCC CGCAGGCGCCGCCACCACGCCCGGCCCCACCGCGAGCAGCGGGAGCGGGGCCGCGACGCCGGAGAGGGTC GACGAGACGACGGAAGTCAAGGCCGCGACGAGAGCGGGCTCGGCGTTTGCCCTCACCACGCCCCCGGCCG GCCTGACCGCCAGCCCCGCCGCCAGCCCCTCCCGTGCCTTTAGCGCGGCCGCCCCGGCCGCCGCTGCGCA GCCGGCCGGAGACACGCCCGCTCGCTTCCGGCGCCAACTGGCGTCGATCCTAGTGCCTCTGTGCGTGCTG GTGCTGCTGCTGCTTGCGCTCTGCGCCGCGACGGTAAACTGCGCGCTGCGCCGCCGCCTGCTGCCGTGCT CTCGGCGCGTTTACAAGCCGCGGACGTGCGCGACATGCGGGAGCGGCACTTGCGCGGGGCGGCCCCCCTG CCGCGGCGCGGCACCGAGCGCCCCAGCCACCGTCGTGGCACTGGGCTCCCGGCCAAAGGCGCCCCCCCTC GCCACCATCAGCGAAGAATAA >NP_045370.1 envelope glycoprotein D [Bovine alphaherpesvirus 1] MQGPTLAVLGALLAVAVSLPTPAPRVTVYVDPPAYPMPRYNYTERWHTTGPIPSPFADGREQPVEVRYAT SAAACDMLALIADPQVGRTLWEAVRRHARAYNATVIWYKIESGCARPLYYMEYTECEPRKHFGYCRYRTP PFWDSFLAGFAYPTDDELGLIMAAPARLVEGQYRRALYIDGTVAYTDFMVSLPAGDCWFSKLGAARGYTF GACFPARDYEQKKVLRLTYLTQYYPQEAHKAIVDYWFMRHGGVVPPYFEESKGYEPPPAADGGSPAPPGD DEAREDEGETEDGAAGREGNGGPPGPEGDGESQTPEANGGAEGEPKPGPSPDADRPEGWPSLEAITHPPP APATPAAPDAVPVSVGIGIAAAAIACVAAAAAGAYFVYTRRRGAGPLPRKPKKLPAFGNVNYSALPG Protective antigen Calves were injected intranasally with either AdCMVgC or AdCMVgD (US6) or a combination of these two recombinants or a commercially available live vaccine for comparison. The highest BHV-1 neutralizing antibody titres were obtained with AdCMVgD (US6) followed by the live vaccine and to a lower extent with the combination of the two recombinants. Calves were protected against intranasal BHV-1 challenge performed 3 weeks after the second immunization [Ref1029:Gogev et al., 2002]. US7 Bovine alphaherpesvirus 1 VO_0011042 4783432 9629887 BoHV1_gp127 KU198480 NP_045371 10320 120286 121435 + envelope glycoprotein I 9.63 37115.17 382 gD family >NC_001847.1:120286-121435 Bovine herpesvirus 1, complete genome GATGCGGTGCCTGTTGCTCTGGATGGTGGTGCTGGCCGCGCGAGCGGCGCCCGCTCGCAGCCTTGTGTAT CGCGGCGAGGCAGTCGGCCTGCGCGCGGACGGCCCCGTAGCGTTCGCTGTCCACCCGACCGACGCAACGC TCGCGCTGCGGGGCCGGCTGATTTTCCTGGAACACCAGCTCCCGGCCGGGCGGCGCTACAACGGGACCGT CGAGCTGCTGCGCTACCACGCCGCGGGCGACTGCTTCGTTATGCTGCAGACGACCGCGTTCGCCTCCTGC CCGCGCGTCGCGAACGACGCCTTTCGCTCCTGCCTGCACGCCGACACGCGCCCCGCTCGCAGCGAGCGGC GCGCGAGCGCCGCGGTCGAAAACCACGTGCTCTTCTCCATCGCCCATCCGCGCCCAATAGACTCAGGGCT CTACTTTCTGCGCGTCGGCATCTACGGCGGCACCGCGGGCAGCGAGCGCCGCCGAGACGTCTTTCCCTTG GCCGCGTTTGTACACAGCTTCGGTGAGCCCGGAGACCCAGAGGCCGCGGCCGCGCACCCCGGCGCCGTCG AGGCAGTCGCGACCCGCTGCGAGCGGGGCCTCGACGCCAGCTCGGCGAGCCTCTACGACCGCGCGCTGGC GGCGTTCCCCGCAGGCGCCGCCACCACGCCCGGCCCCACCGCGAGCAGCGGGAGCGGGGCCGCGACGCCG GAGAGGGTCGACGAGACGACGGAAGTCAAGGCCGCGACGAGAGCGGGCTCGGCGTTTGCCCTCACCACGC CCCCGGCCGGCCTGACCGCCAGCCCCGCCGCCAGCCCCTCCCGTGCCTTTAGCGCGGCCGCCCCGGCCGC CGCTGCGCAGCCGGCCGGAGACACGCCCGCTCGCTTCCGGCGCCAACTGGCGTCGATCCTAGTGCCTCTG TGCGTGCTGGTGCTGCTGCTGCTTGCGCTCTGCGCCGCGACGGTAAACTGCGCGCTGCGCCGCCGCCTGC TGCCGTGCTCTCGGCGCGTTTACAAGCCGCGGACGTGCGCGACATGCGGGAGCGGCACTTGCGCGGGGCG GCCCCCCTGCCGCGGCGCGGCACCGAGCGCCCCAGCCACCGTCGTGGCACTGGGCTCCCGGCCAAAGGCG CCCCCCCTCGCCACCATCAGCGAAGAATAA >NP_045371.1 envelope glycoprotein I [Bovine alphaherpesvirus 1] MRCLLLWMVVLAARAAPARSLVYRGEAVGLRADGPVAFAVHPTDATLALRGRLIFLEHQLPAGRRYNGTV ELLRYHAAGDCFVMLQTTAFASCPRVANDAFRSCLHADTRPARSERRASAAVENHVLFSIAHPRPIDSGL YFLRVGIYGGTAGSERRRDVFPLAAFVHSFGEPGDPEAAAAHPGAVEAVATRCERGLDASSASLYDRALA AFPAGAATTPGPTASSGSGAATPERVDETTEVKAATRAGSAFALTTPPAGLTASPAASPSRAFSAAAPAA AAQPAGDTPARFRRQLASILVPLCVLVLLLLALCAATVNCALRRRLLPCSRRVYKPRTCATCGSGTCAGR PPCRGAAPSAPATVVALGSRPKAPPLATISEE Protective antigen Immunization of calves with truncated gpI (US7) protein induced gpI-specific nasal IgA, IgG1, serum neutralizing antibodies and gpI-specific peripheral lymphocyte proliferation. All immunized calves were protected from clinical disease after BHV-1 challenge. Further, nine of ten immunized calves had no intranasal viral shedding [Ref1031:Gao et al., 1994]. US8 Bovine herpesvirus 1 1487407 9629888 BoHV1_gp42 AJ004801 NP_045372 10320 121713 123505 + envelope glycoprotein E 4.53 56945.75 575 envelope glycoprotein E; the Herpesviridae are non-segmented dsDNA viruses with genomes ranging from 120-230kbp; although herpes viruses vary greatly in sequence identity and homology, they all share four common elements: an envelope, a tegument which is composed of viral enzymes, a capsid of 162 capsomers, and a core composed of genomic DNA;virion envelope glycoproteins bind to cellular receptors; envelope glycoprotein E and glycoprotein I form a heterodimer that play important roles in virus cell-to-cell spread >gi|9629818:121713-123505 Bovine herpesvirus 1, complete genome AATGCAACCCACCGCGCCGCCCCGGCGGCGGTTGCTGCCGCTGCTGCTGCCGCAGTTATTGCTTTTCGGG CTGATGGCCGAGGCCAAGCCCGCGACCGAAACCCCGGGCTCGGCTTCGGTCGACACGGTCTTCACGGCGC GCGCTGGCGCGCCCGTCTTTCTCCCAGGGCCCGCGGCGCGCCCGGACGTGCGCGCCGTTCGCGGCTGGAG CGTCCTCGCGGGCGCCTGCTCGCCGCCCGTGCCGGAGCCCGTCTGCCTCGACGACCGCGAGTGCTTCACC GACGTGGCCCTGGACGCGGCCTGCCTGCGAACCGCCCGCGTGGCCCCGCTGGCCATCGCGGAGCTCGCCG AGCGGCCCGACTCAACGGGCGACAAAGAGTTTGTTCTCGCCGACCCGCACGTCTCGGCGCAGCTGGGTCG CAACGCGACCGGGGTGCTGATCGCGGCCGCAGCCGAGGAGGACGGCGGCGTGTACTTCCTGTACGACCGG CTCATCGGCGACGCCGGCGACGAGGAGACGCAGTTGGCGCTGACGCTGCAGGTCGCGACGGCCGGCGCGC AGGGCGCCGCGCGGGACGAGGAGAGGGAACCAGCGACCGGGCCCACCCCCGGCCCGCCGCCCCACCGCAC GACGACACGCGCGCCCCCGCGGCGGCACGGCGCGCGCTTCCGCGTGCTGCCGTACCACTCCCACGTATAC ACCCCGGGCGATTCCTTTCTGCTATCGGTGCGTCTGCAGTCTGAGTTTTTCGACGAGGCTCCCTTCTCGG CCAGCATCGACTGGTACTTCCTGCGGACGGCCGGCGACTGCGCGCTCATCCGCATATACGAGACGTGCAT CTTCCACCCCGAGGCACCGGCCTGCCTGCACCCCGCCGACGCGCAGTGCAGCTTCGCGTCGCCGTACCGC TCCGAGACCGTGTACAGCCGGCTGTACGAGCAGTGCCGCCCGGACCCTGCCGGTCGCTGGCCGCACGAGT GCGAGGGCGCCGCGTACGCGGCGCCCGTTGCGCACCTGCGTCCCGCCAATAACAGCGTAGACCTGGTCTT TGACGACGCGCCGGCTGCGGCCTCCGGGCTTTACGTCTTTGTGCTGCAGTACAACGGCCACGTGGAAGCT TGGGACTACAGCCTAGTCGTTACTTCGGACCGTTTGGTGCGCGCGGTCACCGACCACACGCGCCCCGAGG CCGCAGCCGCCGACGCTCCCGAGCCAGGCCCACCGCTCACCAGCGAGCCGGCGGGCGCGCCCACCGGGCC CGCGCCCTGGCTTGTGGTGCTGGTGGGCGCGCTTGGACTCGCGGGACTGGTGGGCATCGCAGCCCTCGCC GTTCGGGTGTGCGCGCGCCGCGCAAGCCAGAAGCGCACCTACGACATCCTCAACCCCTTCGGGCCCGTAT ACACCAGCTTGCCGACCAACGAGCCGCTCGACGTGGTGGTGCCAGTTAGCGACGACGAATTTTCCCTCGA CGAAGACTCTTTTGCGGATGACGACAGCGACGATGACGGGCCCGCTAGCAACCCCCCTGCGGATGCCTAC GACCTCGCCGGCGCCCCAGAGCCAACTAGCGGGTTTGCGCGAGCCCCCGCCAACGGCACGCGCTCGAGTC GCTCTGGGTTCAAAGTTTGGTTTAGGGACCCGCTTGAAGACGATGCCGCGCCAGCGCGGACCCCGGCCGC ACCAGATTACACCGTGGTAGCAGCGCGACTCAAGTCCATCCTCCGCTAGGCGCCCCCCCCCCCGCGCGCT GTGCCGTCTGACGGAAAGCACCCGCGTGTAGGGCTGCATATAA >gi|9629888|ref|NP_045372.1| envelope glycoprotein E [Bovine herpesvirus 1] MQPTAPPRRRLLPLLLPQLLLFGLMAEAKPATETPGSASVDTVFTARAGAPVFLPGPAARPDVRAVRGWS VLAGACSPPVPEPVCLDDRECFTDVALDAACLRTARVAPLAIAELAERPDSTGDKEFVLADPHVSAQLGR NATGVLIAAAAEEDGGVYFLYDRLIGDAGDEETQLALTLQVATAGAQGAARDEEREPATGPTPGPPPHRT TTRAPPRRHGARFRVLPYHSHVYTPGDSFLLSVRLQSEFFDEAPFSASIDWYFLRTAGDCALIRIYETCI FHPEAPACLHPADAQCSFASPYRSETVYSRLYEQCRPDPAGRWPHECEGAAYAAPVAHLRPANNSVDLVF DDAPAAASGLYVFVLQYNGHVEAWDYSLVVTSDRLVRAVTDHTRPEAAAADAPEPGPPLTSEPAGAPTGP APWLVVLVGALGLAGLVGIAALAVRVCARRASQKRTYDILNPFGPVYTSLPTNEPLDVVVPVSDDEFSLD EDSFADDDSDDDGPASNPPADAYDLAGAPEPTSGFARAPANGTRSSRSGFKVWFRDPLEDDAAPARTPAA PDYTVVAARLKSILR Virmugen A gE-negative (US8-negative) strain was used as whole-virus antigen in an inactivated virus vaccine. Calves given the vaccine with the highest antigen concentration were adequately protected against challenge; clinical symptoms were virtually absent and challenge virus shedding was significantly reduced as compared with unvaccinated calves [Ref1030:Kaashoek et al., 1995]. Virmugen A deletion of BHV-1 gE gene in strain A is considered as a live attenuated vaccine. After challenge, vaccinated calves were protected against disease and virus shedding was reduced [Ref1806:Kaashoek et al., 1994]. 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