Classical swine fever virus 11096 Classical swine fever, hog cholera Swine fever is is a highly contagious disease of pigs and wild boar (Wiki: Classical swine fever). CSFV is closely related to the ruminant pestiviruses which cause Bovine Viral Diarrhoea (BVDV) and Border Disease (BDV). The effect of different CSFV strains varies widely, leading to a wide range of symptoms. Highly virulent strains correlate with acute, obvious disease and high mortality, including neurological symptoms and hemorrhages within the skin. Less virulent strains can give rise to subacute or chronic infections that may escape detection, while still inducing mortality in fetuses and neonates. Infected piglets birthed from infected but subclinical sows help maintain the disease within a population. Other symptoms can include lethargy, fever, immunosuppression and secondary respiratory infections. The incubation period of CSFV ranges from 2 to 14 days, but symptoms may not be apparent until after 2 to 4 weeks. Animals with an acute infection can survive 2 to 3 months before their eventual death. Eradicating CSF is problematic. Current programmes revolve around rapid detection and diagnosis, and preventive culling, possibly followed by emergency vaccination (ATCvet codes: QI09AA06 for the inactivated viral vaccine, QI09AD04 for the live vaccine). Possible sources for maintaining and introducing infection include the wide transport of pigs and pork products, as well as endemic CSF within wild boar and feral pig populations (Wiki: Classical swine fever). Baboon Papio cynocephalus 9556 Bank vole Clethrionomys glareolus 447135 Bear Ursus americanus 9643 Birds Passeroidea 175121 Brown Trout Salmo trutta 8032 Buffalo Bison bison 9901 Carnivores Vulpes 9625 Cat Felis catus 9685 Catfishes Siluriformes 7995 Cattle Bos taurus 9913 Chicken Gallus gallus 9031 Chimpanzee Pan troglodytes 9598 chinchillas Chinchillidae 10150 Copper Pheasant Syrmaticus soemmerringii 9067 Deer Cervus elaphus 9860 Deer mouse Peromyscus maniculatus 10042 Dog Canis familiaris 9615 Ducks Anas 8835 Ferret Mustela putorius furo 9669 Fish Hyperotreti 117565 Gerbil Gerbillina 10045 Goat Capra hircus 9925 Gray wolf Canis lupus 9612 Guinea pig Cavia porcellus 10141 Hamster Mesocricetus auratus 10036 Horse Equus caballus 9796 Human Homo sapiens 9606 Macaque Macaca fascicularis 9541 Mongolian Gerbil Meriones unguiculatus 10047 Monkey Platyrrhini 9479 Mouse Mus musculus 10090 None None Parrot Psittacidae 9224 Pig Sus scrofa 9823 Rabbit Oryctolagus cuniculus 9986 Rainbow trout Oncorhynchus mykiss 8022 Rat Rattus 10114 Raven Corvus corax 56781 sei whale Balaenoptera borealis 9768 Sheep Ovis aries 9940 Squirrel Spermophilus richardsonii 37591 Tree shrew Tupaiidae 9393 Trouts, salmons & chars Salmoninae 504568 Turkey Meleagris gallopavo 9103 Vole Microtus ochrogaster 79684 Water buffalo Bubalus bubalis 391902 Bayovac CSF E2 Bayovac CSF E2 Bayer Leverkusen VO_0000951 Recombinant vector vaccine Licensed Intramuscular injection (i.m.) Baculovirus recombinant E2 protein without emulsion(Moormann et al., 2000) Intramuscular injection (i.m.) Classical Swine Fever Virus DNA Vaccine pcDNA/E2 with TRIF VO_0004524 DNA vaccine Research pRK-TRIF [Ref2593:Wan et al., 2010] Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction Co-delivery of the DNA vaccine pcDNA/E2 with the TRIF adjuvant enhanced the cellular, not humoral, immune responses induced by DNA vaccines in mice (Wan et al., 2010). VO_0000286 All pigs co-immunized with pcDNA/E2 + pRK-TRIF survived viral challenge., though two pigs in this groups had a slight fever but recovered with 1 or 3 days and the remaining pig in this group had no CSF signs (Wan et al., 2010). All immunized pigs were intramuscularly challenged 2 weeks after the last immunization with 10^5 TCID50 highly virulent CSFV Shimen strain (Wan et al., 2010). VO_0003057 All pigs co-immunized with pcDNA/E2 + pRK-TRIF survived viral challenge., though two pigs in this groups had a slight fever but recovered with 1 or 3 days and the remaining pig in this group had no CSF signs (Wan et al., 2010). All immunized pigs were intramuscularly challenged 2 weeks after the last immunization with 105 TCID50 highly virulent CSFV Shimen strain (Wan et al., 2010). Classical swine fever virus DNA vaccine pCI-gp55 VO_0004340 DNA vaccine Research pCI [Ref2383:Hammond et al., 2001] Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction Vector pCI expressed the gp55/E2 gene from classical swine fever virus (CSFV) (Hammond et al., 2001). VO_0000286 Following challenge with CSFV, 100% of weaned pigs and 75% pre-weaned piglets were protected from disease (Hammond et al., 2001). Classical swine fever virus E(rns) mutant vaccine VO_0002952 Live, attenuated vaccine Research intranasal immunization intranasal immunization Gene mutation This E(rns) mutant is from Classical swine fever virus (Sainz et al., 2008). An E(rns) mutant is attenuated in swine (Sainz et al., 2008). An E(rns) mutant induces protection in swine from challenge with wild type CSVF (Sainz et al., 2008). Classical swine fever virus E1 mutant vaccine VO_0002953 Live, attenuated vaccine Research intranasal immunization intranasal immunization Gene mutation This E1 mutant is from Classical swine fever virus (Fernandez-Sainz et al., 2009). An E1 mutant is attenuated in swine (Fernandez-Sainz et al., 2009). An E1 mutant induced protection in swine from challenge with wild type CSFV (Fernandez-Sainz et al., 2009). Classical swine fever virus E1 protein vaccine VO_0011509 Subunit vaccine Research Baculovirus vector Intramuscular injection (i.m.) Intramuscular injection (i.m.) Classical swine fever virus gp55 encoding E1 Recombinant protein preparation A recombinant virus BacEl[-], which expressed El without a C-terminal TMR, generated a protein that was secreted from the cells. The fraction of this protein that was found to be cell associated had a slightly lower molecular mass (49 to 52 kDa) than wild-type El and remained endo H sensitive. The high-mannose units of the secreted protein were trimmed during transport through the exocytotic pathway to endo H-resistant glycans, resulting in a protein with a lower molecular mass (46 to 48 kDa) (Hulst et al., 1993). Groups of two specific-pathogen-free, 10- to 12-week-old pigs were inoculated intramuscularly with a double water-oil emulsion of immunoaffinity-purified El on day 0. Pigs 1, 2, 3, and 4 were inoculated with 20 ug of El, and pigs 6, 7, 8, and 9 were inoculated with 100 ug of El. After 28 days, pigs 3 and 4 were vaccinated again with 20 ug and pigs 8 and 9 were vaccinated with 100 ug of immunoaffinity-purified El. Pigs 5 and 10 (control pigs) were inoculated on day 0 with a double water-oil emulsion of SF900 medium from Sf21 cells infected with wild-type AcNPV and vaccinated again with the same inoculum on day 28 (Hulst et al., 1993). Intramuscular vaccination of pigs with immunoaffinity-purified E1 (gp55) in a double water-oil emulsion elicited high titers of neutralizing antibodies between 2 and 4 weeks after vaccination at the lowest dose tested (20 micrograms). The vaccinated pigs were completely protected against intranasal challenge with 100 50% lethal doses of HCV (Classical swine fever virus) strain Brescia (Hulst et al., 1993). Pigs of all groups were challenged intranasally on day 42 with 100 50% lethal doses of HCV strain Brescia 456610, a challenge dose that, in unprotected pigs, leads to acute disease characterized by high fever and thrombocytopenia starting at days 3 to 5 and to death at days 7 to 11(Hulst et al., 1993). Classical swine fever virus E2 mutant vaccine VO_0002954 Live, attenuated vaccine Research Oronasal immunization Oronasal immunization Gene mutation This E2 mutant is from Classical swine fever virus (Maurer et al., 2005). An E2 mutant is attenuated in swine (Maurer et al., 2005). An E2 mutant induced significant protection in swine from challenge with wild type CSFV (Maurer et al., 2005). Classical swine fever virus vaccine VAC-E0 VO_0011427 Recombinant vector vaccine Research Recombinant Vaccinia Virus Intradermal injection (i.d.) Intradermal injection (i.d.) Classical swine fever virus envelope glycoprotein E0 Recombinant vector construction A 0.7-kbp fragment (corresponding to nucleotides 1114 to 1838 of CSFV Alfort Tu¨bingen which encompassed the coding sequence for all but 5 amino acids (aa) of CSFV E0 was isolated from plasmid pHCK11 by digestion with BglI and BanI. The missing codons were substituted for with synthetic adaptor oligonucleotides. The 59 adaptor BBA (59GATCCACCAT GGGGGCCCTGT39) linked the BglI site of the 0.7-kbp fragment to the BamHI site of pGS62 and contained a sequence according to Kozak’s rules. Besides the initial methionine, BBA coded for 3 aa: glycine (not found in the CSFV sequence) and alanine and leucine (corresponding to CSFV aa 250 and 251). The 39 adaptor BEA (59GTGCCTATGCCTGAGTTA39) connected the BanI site of the 0.7-kbp fragment to the EcoRI site of pGS62 and encoded amino acids corresponding to glycine 491 to alanine 494 of CSFV as well as a stop codon. After ligation with adaptors BBA and BEA, the 0.7-kbp fragment was introduced into recombination vector pGS62 to derive plasmid pGS62-E0 (König et al., 1995). To generate a VVR vaccine, CVI cells were infected with vaccinia virus strain WR (VAC-WR) at a multiplicity of infection (MOI) of 0.05 and after 1 h were transfected with the respective recombination plasmids by using a mammalian transfection kit. After 48 h of incubation, virus progeny was harvested by repeated freezing and thawing. Selection for a thymidine kinase-negative phenotype on human 143tk2 cells was carried out with medium containing 1% agarose and 100 mg of bromodeoxyuridine per ml. VVR were isolated from thymidine kinase negative virus plaques and were plaque purified twice (König et al., 1995). Pigs were vaccinated with a single dose of VVR (VAC-E0 or VAC-E2) or control strain VAC-WR by three different routes simultaneously (intradermally, intraperitoneally, and intravenously). A total of 5 x 10^7 PFU of VVR was given by each route. Clinical reactions after vaccination were monitored by daily examination (König et al., 1995). Swine vaccinated with VVR expressing E0 and/or E2 resisted a lethal challenge infection with CSFV. Glycoprotein E0 represents a second determinant for the induction of protective immunity against classical swine fever (König et al., 1995). The pigs were challenged intranasally 5 weeks after immunization with 2 x 10^7 50% tissue culture infective doses of CSFV Alfort Tu¨bingen. Clinical symptoms were monitored daily. Blood samples were taken at days 5 and 12 postinfection (p.i.) and at the slaughter of the animals (days 12 to 27 p.i.) (König et al., 1995). Classical swine fever virus vaccine VAC-E2 VO_0011508 Recombinant vector vaccine Research Recombinant Vaccinia Virus Intradermal injection (i.d.) Intradermal injection (i.d.) Classical swine fever virus E2 Recombinant vector construction A 1.5-kbp DNA fragment (representing nucleotides 2433 to 3971 of CSFV Alfort Tu¨bingen) isolated from clone pHCK11 by NheI-HpaI digestion was ligated into plasmid pBR02-16/4. The resulting construct contained the nucleotide sequences for CSFV E2 and PRV-SP. Isolation of the PRV-SP E2 sequence and introduction into vector pGS62 gave rise to pGS62-E2 (König et al., 1995). Pigs were vaccinated with a single dose of VVR (VAC-E0 or VAC-E2) or control strain VAC-WR by three different routes simultaneously (intradermally, intraperitoneally, and intravenously). A total of 5 x 10^7 PFU of VVR was given by each route. Clinical reactions after vaccination were monitored by daily examination (König et al., 1995). Swine vaccinated with VVR expressing E0 and/or E2 resisted a lethal challenge infection with CSFV. Glycoprotein E0 represents a second determinant for the induction of protective immunity against classical swine fever (König et al., 1995). The pigs were challenged intranasally 5 weeks after immunization with 2 x 10^7 50% tissue culture infective doses of CSFV Alfort Tu¨bingen. Clinical symptoms were monitored daily. Blood samples were taken at days 5 and 12 postinfection (p.i.) and at the slaughter of the animals (days 12 to 27 p.i.) (König et al., 1995). DNA vaccine expressing the E2 protein VO_0004514 Live, attenuated vaccine Licensed pcDNA3.1+ [Ref2525:Tarradas et al., 2011] Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction The vaccine is able to increase antibody-mediated responses, while enhancing the T helper cell response associated with the induction of neutralizing antibodies against CSFV (Tarradas et al., 2011). Groups of 3 pigs were used in each experimental group. Briefly, group 1 (pE2) was inoculated with 500 μg of plasmid pE2 in co-injection with 500 μg of pcDNA3.1 (empty plasmid, Invitrogen), group 2 (pE2 + pCCL20) was inoculated with 500 μg pE2 in co-injection with 500 μg of pCCL20, and the control group was injected with PBS. In all cases, three doses were administered intramuscularly in the neck every 12 days. Before every immunization (at days 0, 12 and 24), pigs were bled to follow the CSFV specific immunoresponse (Tarradas et al., 2011). VO_0000286 Immunized animals with E2 DNA vaccine in co-administration with the plasmid containing swine CCL20 developed high titers of neutralizing antibodies against homologous and heterologous CSFV strains, and were totally protected upon a lethal viral challenge (Tarradas et al., 2011). Thirty-six days after the first immunization (pre-challenge), all pigs were challenged with 10^5 DICT50 of CSFV virulent Margarita strain by i.m. injection in the neck (Tarradas et al., 2011). Porcilis Pesti Porcilis Pesti Intervet VO_0000981 Recombinant vector vaccine Licensed Intramuscular injection (i.m.) Baculovirus recombinant E2 protein without emulsion(van, 2003) Intramuscular injection (i.m.) rAdV-SFV-E2 VO_0004700 Recombinant vector vaccine Research Intramuscular injection (i.m.) An adenovirus-vectored Semliki forest virus replicon construct expressing the E2 glycoprotein from CSFV, rAdV-SFV-E2 (Sun et al., 2013). Intramuscular injection (i.m.) VO_0003057 Two immunizations with a dose as low as 6.25×10^5 TCID(50) or a single immunization with a dose of 10^7 TCID(50) rAdV-SFV-E2 provided complete protection against a lethal CSFV challenge (Sun et al., 2013). rORFV-CSFV-E2 VO_0004768 Recombinant vector vaccine Research Intramuscular injection (i.m.) (Voigt et al., 2007) the recombinant parapoxvirus (PPV) Orf virus (ORFV) as a vaccine expressing the CSFV E2 glycoprotein to protect CSFV chellange. Intramuscular injection (i.m.) Recombinant vector construction Using the new recombinant parapoxvirus (PPV) Orf virus (ORFV) as a vaccine expressing the CSFV E2 (Voigt et al., 2007). Pigs were vaccinated with ORFV (Voigt et al., 2007). VO_0003057 Vector virus vaccinated swine were able to cope with the lymphocyte and in particular B-cell depression in peripheral blood after challenge showing no clinical signs and no viremia. Also, the vaccinated swine demonstrated that a single intra-muscular application confers solid protection (Voigt et al., 2007). Pigs were challenged with CSFV (Voigt et al., 2007). rPFV-CSFV-E0 VO_0004748 Recombinant vector vaccine Research Intramuscular injection (i.m.) CSFV E0 gene was amplified from the plasmid pMD18-T-E0 by PCR and cloned into the FPV-P11 and FPV-pSY (Wang et al., 2008). Intramuscular injection (i.m.) Piglets were immunized three times with recombinant Fowlpox virus (Wang et al., 2008). VO_0003057 The protection experiment showed that 75% of piglets immunized three times with recombinant Fowlpox virus were survived, indicating that the recombinant Fowlpox virus was effective (Wang et al., 2008). The pigs were challenged with CSFV (Wang et al., 2008). E(rns) Classical swine fever virus - Brescia 130457 CDD:147625 CDD:152325 CDD:185828 CDD:29472 CDD:152822 CDD:114308 CDD:31829 CDD:28927 CDD:73158 11098 ? Genome polyprotein N-terminal protease (By similarity). /FTId=PRO_0000038062. >gi|130457|sp|P21530.1|POLG_CSFVB RecName: Full=Genome polyprotein; Contains: RecName: Full=N-terminal protease; Short=N-pro; AltName: Full=Autoprotease p20; Contains: RecName: Full=Capsid protein C; Contains: RecName: Full=E(rns) glycoprotein; AltName: Full=gp44/48; Contains: RecName: Full=Envelope glycoprotein E1; AltName: Full=gp33; Contains: RecName: Full=Envelope glycoprotein E2; AltName: Full=gp55; Contains: RecName: Full=p7; Contains: RecName: Full=Non-structural protein 2-3; Short=NS2-3; Contains: RecName: Full=Cysteine protease NS2; AltName: Full=Non-structural protein 2; Contains: RecName: Full=Serine protease NS3; AltName: Full=Non-structural protein 3; Contains: RecName: Full=Non-structural protein 4A; Short=NS4A; Contains: RecName: Full=Non-structural protein 4B; Short=NS4B; Contains: RecName: Full=Non-structural protein 5A; Short=NS5A; Contains: RecName: Full=RNA-directed RNA polymerase; AltName: Full=NS5B MELNHFELLYKTNKQKPMGVEEPVYDVTGRPLFGDPSEVHPQSTLKLPHDRGRGNIKTTLKNLPRRGDCR SGNHLGPVSGIYVKPGPVFYQDYMGPVYHRAPLEFFDEAQFCEVTKRIGRVTGSDGKLYHIYVCIDGCIL LKLAKRGEPRTLKWIRNLTDCPLWVTSCSDDGASASKEKKPDRINKGKLKIAPKEHEKDSRTKPPDATIV VEGVKYQVKKKGKVKGKNTQDGLYHNKNKPPESRKKLEKALLAWAVIAIMLYQPVAAENITQWNLRDNGT NGIQHAMYLRGVSRSLHGIWPEKICKGVPTYLATDTELREIQGMMVASEGTNYTCCKLQRHEWNKHGWCN WYNIDPWIQLMNRTQANLAEGPPSKECAVTCRYDKNADINVVTQARNRPTTLTGCKKGTNFSFAGTVIEG PCNFNVSVEDILYGDHECGSLLQDTALYLVDGMTNTIERARQGAARVTSWLGRQLRIAGKRLEGRSKTWF GAYALSPYCNVTTKIGYIWYTNNCTPACLPKNTKIIGPGKFDTNAEDGKILHEMGGHLSEFLLLSLVVLS DFAPETASALYLILHYVIPQSHEEPEGCDTNQLNLTVELRTEDVIPSSVWNVGKYVCVRPDWWPYETKVA LLFEEAGQVVKLALRALRDLTRVWNSASTTAFLICLIKVLRGQVVQGVIWLLLVTGAQGRLACKEDHRYA ISTTNEIGLHGAEGLTTTWKEYNHNLQLDDGTVKAICMAGSFKVTALNVVSRRYLASLHKDALPTSVTFE LLFDGTSPLTEEMGDDFGFGLCPYDTSPVVKGKYNTTLLNGSAFYLVCPIGWTGVIECTAVSPTTLRTEV VKTFRREKPFPYRRDCVTTTVENEDLFYCKWGGNWTCVKGEPVTYTGGPVKQCRWCGFDFNEPDGLPHYP IGKCILANETGYRIVDSTDCNRDGVVISTEGSHECLIGNTTVKVHALDERLGPMPCRPKEIVSSAGPVRK TSCTFNYAKTLRNRYYEPRDSYFQQYMLKGEYQYWFDLDVTDRHSDYFAEFIVLVVVALLGGRYVLWLIV TYIVLTEQLAAGLQLGQGEVVLIGNLITHTDIEVVVYFLLLYLVMRDEPIKKWILLLFHAMTNNPVKTIT VALLMVSGVAKGGKIDGGWQRLPETNFDIQLALTVIVVAVMLLAKKDPTTVPLVITVATLRTAKITNGLS TDLAIATVSTALLTWTYISDYYKYKTLLQYLISTVTGIFLIRVLKGVGELDLHTPTLPSYRPLFFILVYL ISTAVVTRWNLDIAGLLLQCVPTLLMVFTMWADILTLILILPTYELTKLYYLKEVKIGAERGWLWKTNFK RVNDIYEVDQAGEGVYLFPSKQKTGTITGTMLPLIKAILISCISNKWQFIYLLYLIFEVSYYLHKKIIDE IAGGTNFISRLVAALIEANWAFDNEEVRGLKKFFLLSSRVKELIIKHKVRNEVMVHWFGDEEVYGMPKLV GLVKAATLSKNKHCILCTVCENREWRGETCPKCGRFGPPVTCGMTLADFEEKHYKRIFFREDQSEGPVRE EYAGYLQYRARGQLFLRNLPVLATKVKMLLVGNLGTEVGDLEHLGWVLRGPAVCKKVTEHEKCTTSIMDK LTAFFGVMPRGTTPRAPVRFPTSLLKIRRGLETGWAYTHQGGISSVDHVTCGKDLLVCDTMGRTRVVCQS NNKMTDESEYGVKTDSGCPEGARCYVFNREAVNISGTKGAMVHLQKTGGEFTCVTASGTPAFFDLKNLKG WSGLPIFEASSGRVVGRVKVGKNEDSKPTKLMSGIQTVSKSTTDLTEMVKKITTMNRGEFRQITLATGAG KTTELPRSVIEEIGRHKRVLVLIPLRAAAESVYQYMRQKHPSIAFNLRIGEMKEGDMATGITYASYGYFC QMPQPKLRAAMVEYSFIFLDEYHCSTPEQLAIMGKIHRFSENLRVVAMTATPAGTVTTTGQKHPIEEYIA PEVMKGEDLGPEYLDIAGLKIPVEEMKSNMLVFVPTRNMAVETAKKLKAKGYNSGYYYSGEDPSNLRVVT SQSPYVVVATNAIESGVTLPDLDVVVDTGLKCEKRIRLSPKMPFIVTGLKRMAVTIGEQAQRRGRVGRVK PGRYYRSQETPVGSKDYHYDLLQAQRYGIEDGINITKSFREMNYDWSLYEEDSLMITQLEILNNLLISEE LPMAVKNIMARTDHPEPIQLAYNSYETQVPVLFPKIKNGEVTDSYDNYTFLNARKLGDDVPPYVYATEDE DLAVELLGLDWPDPGNQGTVEAGRALKQVVGLSTAENALLVALFGYVGYQALSKRHIPVVTDIYSIEDHR LEDTTHLQYAPNAIKTEGKETELKELAQGDVQRCMEAMTNYARDGIQFMKSQALKVKETPTYKETMDTVA DYVKKFMEALADSKEDIIKYGLWGTHTALYKSIGARLGNETAFATLVVKWLAFGGESIADHVKQAATDLV VYYIINRPQFPGDTETQQEGRKFVASLLVSALATYTYKSWNYNNLSKIVEPALATLPYAATALKLFAPTR LESVVILSTAIYKTYLSIRRGKSDGLLGTGVSAAMEIMSQNPVSVGIAVMLGVGAVAAHNAIEASEQKRT LLMKVFVKNFLDQAATDELVKESPEKIIMALFEAVQTVGNPLRLVYHVYGVFYKGWEAKELAQRTAGRNL FTLIMFEAVELLGVDSEGKIRQLSSNYILELLYKFRDSIKSSVRQMAISWAPAPFSCDWTPTDDRIGLPQ DNFLRVETKCPCGYKMKAVKNCAGELRLLEEEGSFLCRNKFGRGSRNYRVTKYYDDNLSEIKPVIRMEGH VELYYKGATIKLDFNNSKTILATDKWEVDHSTLVRVLKRHTGAGYCGAYLGEKPNHKHLIERDCATITKD KVCFLKMKRGCAFTYDLSLHNLTRLIELVHKNNLEDKEIPAVTVTTWLAYTFVNEDIGTIKPAFGEKITP EMQEEITLQPAVLVDATDVTVTVVGETPTMTTGETPTTFTSSGPDPKGQQVLKLGVGEGQYPGTNPQRAS LHEAIQSADERPSVLILGSDKATSNRVKTVKNVKVYRGRDPLEVRDMMRRGKILVIALSRVDNALLKFVD YKGTFLTRETLEALSLGRPKKKNITKAEAQWLLRLEDQMEELPDWFAAGEPIFLEANIKHDRYHLVGDIA TIKEKAKQLGATDSTKISKEVGAKVYSMKLSNWVMQEENKQSNLTPLFEELLQQCPPGGQNKTAHMVSAY QLAQGNWMPTSCHVFMGTISARRTKTHPYEAYVKLRELVEEHKMKTLCPGSSLRNDNEWVIGKIKYQGNL RTKHMLNPGKVAEQLHREGHRHNVYNKTIGSVMTATGIRLEKLPVVRAQTDTTNFHQAIRDKIDKEENLQ TPGLHKKLMEVFNALKRPELESSYDAVEWEELERGINRKGAAGFFERKNIGEILDSEKIKVEEIIDNLKK GRNIKYYETAIPKNEKRDVNDDWTAGDFVDEKKPRVIQYPEAKTRLAITKVMYKWVKQKPVVIPGYEGKT PLFQIFDKVKKEWDQFQNPVAVSFDTKAWDTQVTTNDLELIKDIQKYYFKKKWHKFIDTLTMHMSEVPVI TADGEVYIRKGQRGSGQPDTSAGNSMLNVLTMVYAFCEATGVPYKSFDRVAKIHVCGDDGFLITERALGE KFASKGVQILYEAGKPQKITEGDKMKVAYQFADIEFCSHTPIQVRWSDNTSSYMPGRNTTTILAKMATRL DSSGERGTIAYEKAVAFSFLLMYSWNPLIRRICLLVLSTELQVKPGKSTTYYYEGDPISAYKEVIGHNLF DLKRTSFEKLAKLNLSMSVLGAWTRHTSKRLLQDCVNMGVKEGNWLVNADRLVSSKTGNRYVPGEGHTLQ GRHYEELALARKQINSFQGTDRYNLGPIVNMVLRRLRVMMMTLIGRGV Virmugen An N269A/Q mutation in the E(rns) protein created an attenuated mutant in swine. This mutant was able to induce effective protection in swine against challenge with wild type CSFV [Ref1882:Sainz et al., 2008]. E0 VO_0010898 115343287 CDD:294141 CDD:238220 11096 ? envelope glycoprotein E0 7.85 25600.45 313 Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism examined thus far. This family includes RNase Rh, RNase MC1, RNase LE, and self-incompatibility RNases (S-RNases). Plant T2 RNases are expressed during leaf...; cl00208 >ABI94604.1 envelope glycoprotein E0, partial [Classical swine fever virus] ENITQWNLSDNGTNGIQRAMYLRGVNRSLHGIWPEKICKGVPTHLATDTELTEIRGMMDASERTNYTCCR LQRHEWNKHGWCNWYNVDPWIQLMNRTQANLTEGPSDKECAVTCRYDRNADVNVVTQARNRPTTLTGCRK GKNFSFAGTVIEGPCNFNVSVEDILYGDHECGSLLQDTALYLLDGMTNTIENARQGAARVTSWLGRQLST AGKKLERRSKTWFGAYALSPYC Protective antigen Swine vaccinated with VVR expressing E0 and/or E2 resisted a lethal challenge infection with CSFV. Glycoprotein E0 represents a second determinant for the induction of protective immunity against classical swine fever [Ref1065:König et al., 1995]. E1 Classical swine fever virus - Brescia 325460 325461 AAA43843.1 11098 ? envelope glycoprotein E1 >gi|325460|gb|M31768.1|HCVCG3PE Classical swine fever virus - Brescia hog cholera virus protein precursor, gene, complete cds AGTTCATTCTCGTGTACATGATTGGACAAATCAAAATCTCAATTTGGTTCAGGGCCTCCCTCCAGCGACG GCCGAGCTGGGCTAGCCATGCCCACAGTAGGACTAGCAAACGGAGGGACTAGCCGTAGTGGCGAGCTCCC TGGGTGGTCTAAGTCCTGAGTACAGGACAGTCGTCAGTAGTTCGACGTGAGCAGAAGCCCACCTCGAGAT GCTATGTGGACGAGGGCATGCCCAAGACACACCTTAACCTAGCGGGGGTCGTTAGGGTGAAATCACACCA TGTGATGGGAGTACGACCTGATAGGGTGCTGCAGAGGCCCACTATTAGGCTAGTATAAAAATCTCTGCTG TACATGGCACATGGAGTTGAATCATTTTGAACTTTTATACAAAACAAACAAACAAAAACCAATGGGAGTG GAGGAACCGGTATACGATGTAACGGGGAGACCATTGTTTGGAGACCCAAGTGAGGTACACCCACAATCAA CATTGAAGCTACCACATGATAGGGGGAGAGGCAACATCAAAACAACACTGAAGAATCTACCTAGGAGAGG TGACTGCAGGAGTGGCAACCACCTAGGCCCGGTTAGTGGGATATATGTAAAGCCCGGCCCTGTCTTTTAT CAGGACTACATGGGCCCAGTCTATCATAGAGCCCCTCTGGAGTTTTTTGACGAAGCACAGTTCTGTGAGG TGACCAAAAGGATAGGTAGGGTGACAGGTAGTGACGGAAAGCTTTACCATATATACGTGTGCATCGATGG TTGCATCCTGCTGAAGCTAGCCAAGAGGGGCGAGCCAAGAACCCTGAAGTGGATTAGAAATCTCACCGAC TGTCCATTGTGGGTTACCAGTTGTTCTGATGATGGTGCAAGTGCAAGTAAAGAGAAGAAACCAGATAGGA TCAACAAGGGTAAATTAAAGATAGCCCCAAAAGAGCATGAGAAGGACAGCAGGACTAAGCCACCTGATGC TACGATTGTAGTGGAAGGAGTAAAATACCAGGTCAAAAAGAAAGGTAAAGTTAAGGGAAAGAATACCCAA GACGGCCTGTACCACAACAAGAATAAACCACCAGAATCTAGGAAGAAATTAGAAAAAGCCCTATTGGCAT GGGCAGTGATAGCAATTATGTTATACCAACCTGTTGCAGCCGAAAATATAACTCAATGGAACCTGAGAGA CAACGGTACCAATGGTATCCAGCACGCTATGTACCTTAGAGGAGTCAGCAGAAGCTTGCATGGGATCTGG CCAGAAAAAATATGCAAAGGAGTCCCCACCTACCTGGCCACAGACACGGAACTGAGAGAAATACAGGGAA TGATGGTAGCCAGCGAGGGGACAAACTATACGTGCTGTAAGTTACAGAGACATGAATGGAACAAACATGG ATGGTGTAACTGGTATAACATAGACCCCTGGATACAGTTAATGAATAGAACCCAAGCAAACTTGGCAGAA GGCCCTCCGAGCAAGGAGTGCGCCGTGACTTGCAGGTACGATAAAAATGCTGACATTAACGTGGTCACCC AGGCCAGAAACAGGCCAACCACCCTAACTGGCTGCAAGAAAGGGACCAATTTTTCTTTTGCGGGTACAGT TATAGAGGGCCCATGTAATTTCAACGTTTCTGTCGAGGATATCTTATATGGGGATCATGAGTGTGGCAGT CTACTCCAGGATACGGCTCTATACCTAGTAGATGGAATGACCAACACTATAGAGAGAGCCAGGCAGGGAG CCGCGAGGGTGACATCTTGGCTAGGGAGGCAACTCCGCATTGCCGGGAAGAGGTTGGAGGGCAGAAGCAA AACCTGGTTTGGTGCCTATGCCCTATCACCTTATTGTAATGTGACAACGAAAATAGGGTACATATGGTAC ACTAACAACTGTACCCCGGCTTGCCTCCCCAAAAATACAAAGATAATAGGCCCCGGTAAATTTGACACTA ACGCGGAAGACGGAAAGATTCTCCATGAGATGGGGGGCCACCTATCAGAATTTCTGCTGCTCTCTCTGGT CGTTCTGTCTGACTTCGCCCCTGAAACAGCCAGCGCGTTATACCTCATTTTGCACTACGTGATCCCTCAA TCCCATGAAGAACCTGAAGGCTGTGACACAAACCAGCTGAATTTAACAGTGGAACTCAGGACTGAAGACG TGATACCATCATCAGTCTGGAATGTTGGCAAATATGTGTGTGTTAGACCAGACTGGTGGCCATATGAAAC CAAGGTGGCTTTGTTATTTGAAGAGGCAGGACAGGTCGTAAAGTTAGCCTTGCGGGCACTGAGGGATTTA ACCAGGGTCTGGAATAGCGCATCAACCACGGCATTCCTCATCTGCTTGATAAAAGTATTAAGAGGACAGG TCGTGCAAGGTGTGATATGGCTGTTACTGGTAACTGGGGCACAAGGCCGGCTAGCCTGCAAGGAAGATCA CAGGTACGCTATATCAACAACCAATGAGATAGGGCTACATGGGGCCGAAGGTCTCACTACCACCTGGAAA GAATACAACCACAATTTGCAACTGGATGATGGGACCGTCAAGGCCATCTGCATGGCAGGTTCCTTTAAAG TCACAGCACTTAATGTGGTTAGTAGGAGGTATCTGGCATCATTACATAAGGACGCTTTACCCACTTCCGT GACATTCGAGCTCCTGTTCGACGGGACCAGCCCATTGACCGAGGAAATGGGAGATGACTTCGGGTTCGGA CTGTGTCCGTATGATACGAGCCCTGTAGTCAAGGGAAAATACAACACAACCTTGTTGAATGGTAGTGCAT TCTACCTAGTTTGCCCAATAGGGTGGACGGGTGTTATAGAGTGCACGGCAGTGAGCCCGACAACTCTGAG AACAGAAGTGGTAAAGACCTTCAGAAGAGAGAAACCCTTTCCGTACAGAAGGGATTGTGTGACCACTACA GTGGAAAATGAAGATCTATTCTACTGTAAATGGGGGGGCAATTGGACATGTGTGAAAGGTGAACCAGTGA CCTACACGGGGGGGCCAGTAAAACAATGCAGATGGTGTGGCTTCGACTTCAATGAGCCTGACGGACTCCC ACACTACCCCATAGGTAAGTGCATTTTGGCAAATGAGACAGGTTACAGAATAGTGGATTCAACGGACTGT AACAGAGATGGCGTTGTAATCAGCACAGAGGGGAGTCATGAGTGCTTGATTGGTAACACAACTGTCAAGG TGCATGCATTAGATGAAAGACTAGGCCCTATGCCATGCAGGCCTAAGGAGATCGTCTCTAGTGCGGGACC TGTAAGGAAAACTTCCTGTACATTCAACTACGCAAAAACTCTGAGGAACAGGTATTATGAGCCCAGGGAC AGCTATTTCCAACAATATATGCTCAAGGGCGAGTATCAGTACTGGTTTGATCTGGATGTGACCGACCGCC ACTCAGATTACTTCGCAGAATTCATTGTCTTGGTGGTGGTGGCACTGTTGGGAGGAAGATATGTCCTGTG GCTAATAGTGACCTACATAGTTCTAACAGAACAACTCGCCGCTGGTCTACAGTTAGGCCAGGGTGAGGTA GTGTTAATAGGGAACTTAATCACCCACACAGATATTGAGGTTGTAGTATATTTCTTACTGCTCTATTTGG TCATGAGAGATGAGCCTATAAAGAAATGGATACTACTGCTGTTCCATGCTATGACCAACAATCCAGTTAA GACCATAACAGTGGCACTGCTCATGGTTAGCGGGGTTGCCAAGGGTGGAAAGATAGATGGTGGTTGGCAG CGGCTGCCGGAGACCAACTTTGATATCCAACTCGCGCTGACAGTTATAGTAGTCGCTGTGATGTTGCTGG CAAAGAAAGATCCGACTACCGTCCCCTTGGTTATAACGGTGGCAACCCTGAGAACGGCTAAGATAACTAA TGGACTTAGTACAGATCTAGCCATAGCTACAGTGTCAACAGCTTTGCTAACCTGGACCTACATTAGTGAC TATTATAAATACAAGACCTTGCTACAGTACCTTATTAGCACAGTGACAGGTATCTTCTTGATAAGGGTAC TGAAGGGGGTAGGTGAGTTAGATTTACACACCCCAACCTTACCATCTTACAGACCCCTCTTCTTCATCCT CGTGTACCTCATTTCCACTGCAGTGGTAACAAGATGGAATCTGGACATAGCCGGATTGCTGCTGCAGTGT GTCCCAACCCTTTTAATGGTTTTCACGATGTGGGCAGACATCCTTACCCTGATCCTCATACTGCCTACTT ACGAGTTGACAAAACTATATTACCTCAAGGAAGTGAAGATTGGGGCAGAAAGGGGCTGGTTGTGGAAGAC CAACTTCAAGAGGGTAAATGACATATACGAAGTTGACCAAGCTGGTGAGGGGGTGTACCTTTTCCCATCA AAACAAAAGACAGGTACAATAACAGGTACTATGTTGCCACTGATCAAAGCCATACTCATAAGTTGCATCA GCAATAAGTGGCAATTTATATATCTATTGTACTTGATATTCGAAGTGTCTTACTACCTTCACAAGAAGAT CATAGATGAAATAGCAGGAGGGACCAACTTCATCTCGAGACTTGTAGCCGCTCTGATCGAAGCCAATTGG GCCTTTGACAACGAAGAAGTTAGAGGTTTAAAGAAGTTCTTCTTGCTGTCTAGTAGGGTTAAAGAACTGA TCATCAAACACAAAGTGAGGAATGAAGTGATGGTCCACTGGTTTGGCGACGAAGAGGTCTATGGGATGCC GAAGCTGGTTGGCTTAGTCAAGGCAGCAACACTGAGTAAAAATAAACATTGTATTTTGTGCACCGTCTGT GAAAACAGAGAGTGGAGAGGAGAAACCTGCCCAAAATGCGGCCGTTTTGGGCCACCAGTGACCTGTGGCA TGACCCTAGCCGACTTTGAAGAAAAACACTATAAGAGGATTTTCTTTAGAGAGGATCAATCAGAAGGGCC GGTTAGGGAGGAGTATGCAGGGTATCTGCAATATAGAGCCAGAGGGCAATTATTCCTGAGGAATCTCCCG GTGCTAGCAACAAAAGTCAAGATGCTCCTGGTCGGAAATCTTGGGACGGAGGTGGGGGATTTGGAACACC TTGGCTGGGTGCTCAGAGGGCCTGCCGTTTGCAAGAAGGTTACCGAACATGAGAAATGCACCACATCCAT AATGGACAAATTAACTGCTTTCTTCGGTGTTATGCCAAGGGGCACCACACCTAGAGCCCCTGTGAGATTC CCCACCTCTCTCTTAAAGATAAGAAGGGGGCTGGAAACTGGCTGGGCGTACACACACCAAGGTGGCATCA GTTCAGTGGACCATGTCACTTGTGGGAAAGACTTACTGGTATGTGACACTATGGGCCGGACAAGGGTTGT TTGCCAATCAAATAACAAGATGACAGACGAGTCCGAGTATGGAGTTAAAACTGACTCCGGATGCCCGGAG GGAGCTAGGTGTTACGTGTTCAACCGAGAGGCAGTTAATATATCCGGGACTAAAGGAGCTATGGTCCACT TACAAAAAACTGGAGGAGAATTCACCTGTGTGACAGCATCAGGGACTCCGGCCTTCTTTGATCTCAAGAA CCTCAAAGGCTGGTCAGGGCTACCGATATTTGAGGCATCAAGTGGAAGAGTAGTCGGCAGGGTTAAGGTC GGGAAGAATGAGGACTCTAAACCAACCAAGCTTATGAGTGGAATACAAACAGTCTCCAAAAGTACCACAG ACTTGACAGAAATGGTAAAGAAAATAACAACCATGAACAGGGGAGAATTCAGACAAATAACCCTTGCCAC AGGTGCCGGAAAAACCACGGAACTCCCTAGATCAGTCATAGAAGAGATAGGAAGGCATAAGAGGGTCTTG GTCTTGATCCCTCTGAGGGCGGCAGCAGAGTCAGTATACCAATATATGAGACAAAAACACCCAAGCATAG CATTCAACTTGAGGATAGGGGAGATGAAGGAAGGGGACATGGCCACAGGGATAACCTATGCCTCATATGG TTACTTCTGTCAGATGCCACAACCTAAGCTGCGAGCCGCGATGGTTGAGTACTCCTTCATATTCCTTGAT GAGTACCACTGTTCCACCCCCGAACAATTGGCTATCATGGGAAAGATCCACAGATTTTCAGAGAACCTGC GGGTAGTAGCCATGACCGCAACACCAGCAGGCACGGTAACAACTACAGGGCAAAAACACCCTATAGAAGA ATACATAGCCCCAGAAGTGATGAAGGGGGAAGACTTAGGTCCAGAGTACTTGGACATAGCTGGACTAAAG ATACCAGTAGAGGAGATGAAGAGTAACATGCTGGTCTTTGTGCCCACAAGGAACATGGCTGTAGAGACGG CAAAGAAACTGAAAGCTAAGGGTTATAACTCAGGCTACTATTATAGTGGAGAGGATCCATCTAACCTGAG GGTGGTAACATCACAGTCCCCGTACGTGGTGGTAGCAACCAACGCAATAGAATCAGGTGTTACTCTCCCA GACTTGGATGTGGTCGTCGACACAGGGCTTAAGTGTGAAAAGAGGATACGGCTGTCACCTAAGATGCCCT TCATAGTGACGGGCCTGAAGAGAATGGCTGTCACGATTGGGGAACAAGCCCAGAGAAGGGGGAGAGTTGG GAGAGTGAAGCCTGGGAGATACTACAGGAGTCAAGAAACCCCCGTTGGTTCCAAAGATTACCATTACGAC CTACTGCAAGCACAGAGGTACGGTATAGAAGATGGGATAAACATCACCAAATCTTTTAGAGAGATGAATT ATGATTGGAGCCTTTATGAGGAGGATAGTCTGATGATTACACAATTGGAAATCCTCAACAATCTGTTGAT ATCAGAAGAGCTACCAATGGCAGTAAAAAATATAATGGCCAGGACTGACCACCCAGAACCAATCCAACTG GCGTACAACAGCTACGAAACGCAGGTGCCAGTTCTATTCCCAAAAATAAAAAATGGAGAGGTGACTGACA GTTACGATAACTATACCTTCCTCAACGCAAGAAAGCTGGGGGATGATGTACCACCCTACGTGTATGCCAC AGAGGATGAGGACTTAGCGGTAGAGCTGCTGGGCTTAGACTGGCCGGACCCTGGGAACCAAGGAACCGTG GAGGCTGGTAGAGCACTAAAACAAGTAGTTGGTCTATCAACAGCTGAGAACGCCCTGTTAGTAGCTTTAT TCGGCTATGTAGGATATCAGGCACTCTCAAAGAGGCATATACCAGTAGTCACAGACATATATTCAATTGA AGATCACAGGTTGGAAGACACCACACACCTACAGTATGCCCCGAATGCTATCAAGACGGAGGGGAAGGAG ACAGAATTGAAGGAGCTAGCTCAGGGGGATGTGCAGAGATGTATGGAAGCTATGACTAATTATGCAAGAG ATGGCATCCAATTCATGAAGTCTCAGGCACTGAAAGTGAAAGAAACCCCCACTTACAAAGAGACAATGGA CACCGTGGCGGACTATGTAAAGAAGTTCATGGAGGCACTGGCGGACAGCAAAGAAGACATCATAAAATAT GGGTTGTGGGGGACGCACACAGCCTTATATAAGAGCATCGGTGCTAGGCTTGGGAACGAGACTGCGTTCG CTACCCTGGTCGTGAAATGGCTGGCATTTGGGGGAGAATCAATAGCAGACCATGTCAAACAAGCGGCCAC AGACTTGGTCGTTTACTATATCATCAACAGACCTCAGTTCCCAGGAGACACGGAGACACAACAGGAAGGA AGGAAATTTGTAGCCAGCCTACTGGTCTCAGCCCTGGCTACTTACACTTACAAAAGCTGGAATTACAATA ATCTGTCCAAGATAGTTGAACCGGCTTTGGCTACTCTGCCCTATGCCGCCACAGCTCTCAAGCTATTCGC CCCCACTCGATTGGAGAGCGTTGTCATACTGAGTACCGCAATCTACAAAACCTACCTATCAATCAGGCGC GGAAAAAGCGATGGTTTGCTAGGCACAGGGGTTAGTGCGGCTATGGAAATCATGTCACAAAACCCAGTAT CTGTGGGTATAGCGGTCATGCTAGGGGTGGGGGCCGTAGCGGCCCACAATGCAATCGAAGCCAGTGAGCA GAAGAGAACACTACTCATGAAAGTTTTTGTAAAGAACTTCTTGGATCAGGCAGCCACTGATGAATTAGTC AAGGAGAGCCCTGAGAAAATAATAATGGCTTTGTTTGAAGCAGTGCAGACAGTCGGCAACCCTCTTAGAC TGGTATACCACGTTTACGGAGTTTTTTACAAAGGGTGGGAGGCAAAAGAGTTGGCCCAAAGGACAGCCGG TAGGAATCTTTTCACTTTGATAATGTTTGAGGCTGTGGAACTACTGGGAGTAGATAGCGAAGGAAAGATC CGCCAGCTATCAAGCAATTACATACTAGAGCTCCTGTATAAGTTCCGTGACAGTATCAAGTCCAGCGTGA GGCAGATGGCAATCAGCTGGGCCCCTGCCCCTTTTAGTTGTGATTGGACACCGACGGATGACAGAATAGG GCTTCCCCAAGATAATTTCCTCCGAGTGGAGACAAAATGCCCCTGTGGTTACAAGATGAAAGCAGTTAAG AATTGTGCTGGGGAGTTGAGACTCTTAGAGGAGGAAGGCTCATTTCTCTGCAGGAATAAATTCGGGAGAG GTTCACGGAACTACAGGGTGACAAAATACTATGATGACAATCTATCAGAAATAAAGCCAGTGATAAGAAT GGAAGGACATGTGGAACTCTACTACAAGGGAGCCACTATTAAACTGGATTTCAACAACAGTAAAACAATA TTGGCAACCGATAAATGGGAGGTCGATCACTCCACTCTGGTCAGGGTGCTCAAGAGGCACACAGGGGCTG GATATTGTGGGGCATACCTGGGTGAGAAACCGAACCACAAACATCTGATAGAGAGGGACTGCGCAACCAT CACCAAAGATAAGGTTTGTTTTCTCAAGATGAAGAGAGGGTGTGCATTTACTTATGACTTATCCCTTCAC AACCTTACCCGGCTGATTGAATTGGTACACAAGAATAACTTGGAAGACAAAGAGATTCCTGCCGTTACGG TCACAACCTGGCTGGCTTACACATTTGTAAATGAAGATATAGGGACCATAAAACCAGCCTTCGGGGAGAA AATAACACCAGAGATGCAGGAGGAGATAACCTTGCAGCCTGCTGTATTGGTGGATGCAACTGACGTGACC GTGACCGTGGTAGGGGAAACCCCTACTATGACTACAGGGGAGACCCCAACAACGTTCACCAGCTCAGGTC CAGACCCGAAAGGCCAACAAGTTTTAAAACTGGGTGTAGGTGAAGGCCAATACCCCGGGACTAATCCACA GAGAGCAAGCCTGCACGAAGCCATACAAAGCGCAGATGAAAGGCCCTCTGTGCTGATATTGGGGTCTGAT AAAGCCACCTCTAATAGAGTGAAAACTGTAAAGAATGTGAAGGTATACAGAGGCAGGGACCCACTAGAAG TGAGAGATATGATGAGGAGGGGAAAGATCCTAGTCATAGCCCTGTCTAGGGTTGATAATGCTCTATTGAA ATTTGTAGATTACAAAGGCACCTTTTTAACTAGAGAGACCCTGGAGGCATTAAGTTTGGGTAGGCCAAAA AAGAAAAACATAACCAAGGCAGAAGCACAGTGGTTGCTGCGCCTCGAAGACCAAATGGAAGAGCTACCCG ATTGGTTCGCAGCCGGGGAACCCATTTTTCTAGAGGCCAATATTAAACATGACAGGTATCATCTGGTAGG GGATATAGCTACTATCAAAGAGAAAGCCAAACAATTGGGGGCTACAGACTCTACAAAGATATCCAAGGAG GTTGGTGCAAAAGTATATTCTATGAAATTGAGTAATTGGGTGATGCAAGAAGAAAACAAACAGAGCAACT TGACCCCCTTATTTGAAGAGCTCCTACAGCAGTGTCCACCCGGAGGCCAAAACAAAACTGCACATATGGT CTCTGCTTACCAACTAGCTCAAGGGAACTGGATGCCAACCAGCTGCCATGTTTTTATGGGGACCATATCT GCCAGAAGGACTAAGACCCATCCATATGAAGCATATGTCAAGTTAAGGGAGTTGGTAGAGGAACACAAGA TGAAAACATTGTGTCCCGGATCAAGTCTGCGTAACGACAATGAATGGGTAATTGGCAAGATCAAATACCA GGGCAACCTGAGGACCAAACACATGTTGAACCCCGGCAAGGTGGCAGAGCAACTGCACAGAGAAGGACAC AGACACAATGTGTATAACAAGACAATAGGCTCAGTGATGACAGCTACTGGCATCAGGTTGGAGAAGTTGC CCGTGGTTAGGGCCCAGACAGACACAACCAACTTCCACCAAGCAATAAGGGATAAGATAGACAAGGAAGA GAATCTACAGACCCCGGGTTTACATAAGAAACTAATGGAAGTTTTCAATGCATTGAAACGACCCGAGTTA GAGTCCTCCTATGACGCTGTGGAATGGGAGGAATTGGAGAGAGGAATAAACAGAAAGGGTGCTGCTGGTT TCTTTGAACGCAAAAACATAGGGGAGATATTGGATTCAGAGAAAATTAAAGTAGAAGAGATTATTGACAA TCTGAAAAAGGGTAGAAATATCAAATACTATGAAACCGCAATCCCAAAAAATGAAAAGAGGGATGTCAAT GATGACTGGACCGCAGGTGACTTTGTGGACGAGAAGAAACCCAGAGTCATACAATACCCTGAAGCAAAAA CAAGGCTGGCCATCACCAAGGTGATGTATAAGTGGGTGAAGCAGAAGCCAGTAGTCATACCCGGGTATGA AGGGAAGACACCTCTGTTCCAAATTTTTGACAAAGTAAAGAAGGAATGGGATCAATTCCAAAATCCAGTG GCAGTGAGCTTCGACACTAAGGCGTGGGACACCCAGGTGACCACAAATGATCTGGAGCTGATAAAGGACA TACAAAAGTACTACTTCAAGAAGAAATGGCATAAATTTATTGACACCCTGACTATGCATATGTCAGAAGT ACCCGTAATCACTGCTGATGGGGAGGTGTATATAAGGAAAGGGCAAAGAGGTAGTGGACAGCCCGACACA AGCGCAGGCAACAGCATGCTAAATGTGTTAACAATGGTTTATGCCTTCTGCGAGGCCACAGGGGTACCCT ACAAGAGTTTTGACAGGGTGGCAAAAATTCATGTGTGTGGGGACGATGGTTTCCTGATCACAGAGAGAGC TCTCGGCGAGAAATTCGCAAGCAAGGGAGTCCAAATCCTGTATGAAGCTGGGAAGCCCCAGAAGATCACT GAAGGGGACAAAATGAAAGTGGCCTACCAATTTGCTGATATTGAGTTTTGCTCCCATACACCAATACAAG TAAGGTGGTCAGATAACACTTCTAGCTACATGCCAGGGAGAAATACAACCACAATCCTGGCTAAAATGGC CACAAGGTTAGATTCCAGTGGTGAGAGGGGTACCATAGCGTACGAGAAAGCAGTAGCATTCAGCTTCCTG CTAATGTATTCCTGGAACCCACTAATCAGAAGGATTTGCTTATTGGTACTATCAACTGAACTGCAAGTGA AACCAGGGAAGTCAACCACTTACTATTATGAAGGGGACCCGATATCTGCCTACAAGGAAGTCATCGGCCA CAATCTTTTCGATCTCAAGAGAACAAGCTTCGAGAAGCTGGCCAAGTTAAATCTCAGCATGTCCGTACTC GGGGCCTGGACTAGACACACCAGCAAAAGACTACTACAAGACTGTGTCAATATGGGTGTTAAAGAGGGCA ACTGGTTAGTCAATGCAGACAGACTGGTGAGTAGTAAGACTGGAAATAGGTATGTACCTGGAGAAGGCCA CACCCTGCAAGGGAGACATTATGAAGAACTGGCGTTGGCAAGAAAACAGATCAACAGCTTCCAAGGGACA GACAGGTACAATCTAGGCCCAATAGTCAACATGGTGTTAAGGAGGCTGAGAGTCATGATGATGACCCTGA TAGGGAGAGGGGTATGAGTGCGGGTGACCCGCGATCTGGACCCGTCAGTAGGACCCTATTGTAGATAACA CTAATTTTTTATTTATTTAGATATTACTATTTATTTATTTATTTATTTATTGAATGAGTAAGAACTGGTA CAAACTACCTCATGTTACCACACTACACTCATTTTAACAGCACTTTAGCTGGAAGGAAAATTCCTGACGT CCACAGTTGGACTAAGGTAATTTCTAACGGCCC Virmugen An N to A amino acid substitution was made in the N594 site of the E1 glycoprotein, resulting in attenuation of the virus in swine. This attenuated virus induced protection against challenge with wild type CSFV [Ref1881:Fernandez-Sainz et al., 2009]. E2 Classical swine fever virus strain LPC VO_0010897 50403916 CDD:292945 11096 373 12069 + E2 protein 8.23 6837.14 127 Pestivirus envelope glycoprotein E2; pfam16329 >AAT76713.1 E2 protein, partial [Classical swine fever virus] TTTWKEYTHDLQLNDGTVKATCVAGSFKVTALNVVSRRYLASLHKKALPTSVTFELLFDGTNP Protective antigen Pigs were subjected to challenge infection with a dose of 1x10(5)TCID(50) (50% tissue culture infective dose) virulent CSFV strain. At 1 week post challenge infection, all of the yE2-immunized pigs were alive and without symptoms or signs of CSF. The yeast-expressed E2 protein retains correct immunogenicity and is able to induce a protective immune response against CSFV infection [Ref1064:Lin et al., 2009]. In a seperate study, swine vaccinated with VVR expressing E0 and/or E2 resisted a lethal challenge infection with CSFV. Glycoprotein E0 represents a second determinant for the induction of protective immunity against classical swine fever [Ref1065:König et al., 1995]. E2 Classical swine fever virus 920146 12657942 PestiV2gp1 AF326963 NP_075354 11096 373 12069 + polyprotein 8.53 408166.02 3898 >NC_002657.1:373-12069 Classical swine fever virus, complete genome CATGGAGTTGAATCATTTTGAACTTTTATACAAAACAAACAAACAAAAACCAATGGGAGTGGAGGAACCG GTATACGATGCCACGGGGAGGCCATTGTTTGGAGACCCGAGTGAGGTACACCCACAATCAACACTGAAGC TACCACATGATAGGGGGAGAGGTAACATCAAAACAACACTGAAGAACCTACCTAGGAAAGGCGACTGCAG GAGTGGCAACCATCTAGGCCCGGTTAGTGGGATATATGTAAAGCCCGGCCCTGTCTTTTATCAGGACTAC ATGGGCCCGGTCTACCATAGAGCCCCTCTAGAGTTTTTTAACGAAGCGCAGTTTTGCGAGGTGACCAAAA GGATAGGTAGGGTGACAGGTAGTGACGGAAAGCTTTACCATATATATGTGTGCATCGATGGTTGCATACT GCTGAAGCTAGCCAAGAGGGACGAGCCAAGAACCCTGAAGTGGATTAGAAATTTCACCGACTGTCCATTG TGGGTTACCAGTTGCTCTGATGATGGCGCAAGTGGAAGTAAAGAGAAGAAGCCAGATAGGATCAACAAAG GCAAATTAAAAATAGCCCCAAAAGAGCATGAGAAGGACAGCAGAACTAAGCCACCTGACGCTACGATTGT AGTGGAAGGAGTAAAATACCAGGTTAAAAAGAAGGGTAAAGTTAAAGGAAAGAGTACCCAAGACGGCCTG TACCACAACAAGAATAAACCACCAGAATCTAGGAAGAAATTAGAAAAAGCCCTATTGGCATGGGCGGTAA TAGCAATTATGTTGTACCAACCAGTTGAAGCCGAAAATATAACTCAATGGAACCTGAGTGACAACGGCAC TAATGGTATCCAGCATGCTATGTACCTTAGAGGGGTTAGCAGGAGCTTGCATGGGATCTGGCCGGAAAAA ATATGCAAAGGAGTCCCCACCTACCTGGCCACAGACACGGAACTGAAAGAAATACAGGGAATGATGGATG CCAGCGAGGGGACAAACTATACGTGCTGTAAGTTACAGAGACATGAATGGAACAAACATGGATGGTGTAA CTGGTACAATATAGACCCCTGGATACAGTTGATGAATAGAACCCAAGCAAACTTGGCAGAAGGCCCTCCG GCCAAGGAGTGCGCTGTGACTTGCAGGTACGATAAAGATGCTGACATCAACGTGGTCACCCAGGCCAGAA ACAGGCCAACAACCCTGACCGGTTGCAAGAAAGGAAAAAATTTTTCTTTTGCGGGTACAGTTATAGAGGG CCCATGTAATTTCAATGTTTCCGTGGAGGATATCTTGTATGGGGATCATGAGTGCGGCAGTTTGCTTCAG GACACGGCTCTGTACCTAGTGGATGGAATGACCAACACTATAGAGAATGCCAGACAGGGAGCAGCGAGGG TAACATCTTGGCTCGGGAGGCAACTCAGCACTGCCGGGAAGAGGTTGGAGGGTAGAAGCAAAACCTGGTT TGGTGCCTATGCCCTATCGCCTTACTGTAATGTAACAAGCAAAATAGGGTACATATGGTACACTAACAAC TGCACCCCGGCTTGCCTCCCCAAAAATACAAAGATAATAGGCCCCGGAAAATTTGACACTAACGCGGAAG ACGGAAAGATTCTCCATGAGATGGGGGGTCACCTATCAGAATTTCTGCTGCTCTCTCTGGTTGTTCTGTC TGACTTCGCCCCTGAAACAGCCAGCGCGTTATACCTCATTTTGCACTACATGATTCCTCAATCCCATGAA GAACCTGAAGGCTGCGACACAAACCAGCTGAATCTAACAGTGGAACTCAGGACTGAAGACGTAATACCGT CATCAGTCTGGAATGTTGGCAAATATGTGTGTGTTAGACCAGACTGGTGGCCATATGAAACCAAGGTGGC TTTGTTATTTGAAGAGGCAGGACAGGTCGTAAAATTAGCCTTACGAGCGCTGAGGGATTTAACCAGGGTC TGGAATAGCGCATCAACCACGGCATTCCTCATCTGCTTGATAAAAGTATTAAGAGGACAGATCGTGCAAG GTGTGATATGGCTGCTACTAGTAACTGGGGCACAAGGCCGGCTAGCCTGCAAGGAAGATTACAGGTACGC AATATCATCGACCAATGAGATAGGGCTACTCGGGGCCGAAGGTCTCACCACCACCTGGAAAGAATACAAC CACGATTTGCAACTGAATGACGGGACCGTTAAGGCCATTTGCGTGGCAGGTTCCTTTAAAGTCATAGCAC TTAATGTGGTCAGTAGGAGGTATTTGGCATCATTGCATAAGGAGGCTTCACTCACTTCCGTGACATTTGA GCTCCTGTTCGACGGGACCAACCCATCAACTGAGGAAATGGGAGATGACTTCGGGTTCGGGCTGTGCCCG TTCGATACGAGTCCTGTTGTCAAGGGAAAGTACAATACAACCTTGTTGAACGGTAGTGCTTTCTATCTTG TCTGCCCAATAGGGTGGACGGGTGTCATAGAGTGCACAGCAGTGAGCCCAACAACTCTGAGAACAGAAGT GGTAAAGACCTTCAGGAGAGACAAGCCCTTTCCGCACAGAATGGATTGTGCGACCACCACAGTGGAAAAT GGAGATTTATTCTACTGTAAGTTGGGGGGCAACTGGACATGTGTGAAAGGTGAACCAGTGGTCTACACGG GGGGGCTAGTAAAACAATGCAGATGGTGTGGCTTCGACTTCAATGAGCCCGACGGACTCCCGCACTACCC CATAGGTAAGTGCATCTTGGTAAATGAGACAGGTTACAGAATAGTAGATTCAACGGACTGTAACAGAGAT GGCGTTGTAATCAGCACAGATGGGAGTCATGAGTGCTTGATCGGTAACACAACTGTCAAGGTGCATGCAT CAGATGAAAGACTGGGCCCTATGCCATGCAGACCCAAAGAGATTGTCTCTAGTGCAGGACCTGTAAGGAA AACTTCCTGTACATTCAACTACGCAAAAACTTTGAAGAACAAGTACTATGAGCCCAGGGACAGCTACTTC CAGCAATATATGCTTAAGGGCGAGTATCAGTACTGGTTTGACCTGGACGTGACTGACCGCCACTCAGATT ACTTCGCAGAATTTGTCGTCTTGGTAGTGGTAGCACTGTTAGGAGGAAGATATGTCCTGTGGCTAATAGT GACCTACATAGTTCTAACAGAACAACTCGCCGCTGGTTTACCATTGGGCCAGGGTGAGGTAGTGTTGATA GGGAACTTAATTACCCACACAGACATTGAGGTCGTAGTATATTTCTTACTACTCTATTTGGTCATGAGGG ATGAGCCTATAAAGAAATGGATACTGCTGCTGTTCCATGCTATGACTAACAATCCAGTCAAGACCATAAC AGTGGCATTGCTTATGGTTAGTGGGGTTGCCAAGGGTGGAAAGATAGACGGCGGTTGGCAGCGGCTGCCA GAGACCAGCTTTGACATCCAACTCGCGCTGACAGTTATAGTAGTCGCTGTGATGTTACTGGCAAAGAGAG ATCCAACTACTGTCCCCTTGGTTATAACAGTGGCAACCCTGAGAACGGCTAAGATGACTAATGGACTTAG CACGGATATAGCCATAGCTACAGTGTCAACAGCGTTGCTAACCTGGACCTACATTAGTGACTATTATAGA TACAAGACTTGGCTACAGTACCTTATTAGCACAGTGACAGGTATCTTCTTAATAAGGGTACTGAAGGGAA TAGGTGAGTTGGATTTACACACTCCAACCTTGCCATCTTACAGACCCCTCTTCTTCATTCTCGTGTACCT CATTTCCACTGCAGTGGTAACAAGATGGAATCTGGACATAGCCGGATTGCTGTTGCAGTGTGTCCCAACC CTTTTGATGGTTTTTACGATGTGGGCAGACATTCTCACCCTGATCCTCATACTGCCCACTTACGAGCTAA CAAAACTATATTACCTCAAGGAAGTGAAGACTGGGGCAGAAAAGGGCTGGTTATGGAAGACCAACTTCAA GAGGGTAAACGACATATACGAAGTTGACCAATCTGGTGAAGGGGTTTACCTTTTCCCGTCAAAACAAAAG ACAAGTTCAATAACAGGTACCATGTTGCCATTGATCAAAGCCATACTCATCAGCTGCATCAGTAATAAGT GGCAGTTCATATATCTATTGTACTTGATATTTGAAGTGTCTTACTACCTCCACAAGAAGATCATAGATGA AATAGCAGGAGGGACCAACTTCATCTCAAGACTTGTAGCCGCTTTGATCGAAGCCAATTGGGCCTTTGAC AACGAAGAAGTTAGGGGTTTAAAGAAGTTCTTCCTGTTGTCTAGTAGGGTTAAAGAACTGATCATCAAAC ACAAAGTGAGGAATGAAGTAATGGTCCACTGGTTTGGTGACGAAGAGGTTTATGGGATGCCAAAGTTGGT TGGCTTAGTCAAGGCAGCAACATTGAGTAAAAATAAACATTGTATTTTGTGCACCGTCTGTGAAGACAGA GAGTGGAGAGGAGAAACCTGCCCAAAATGCGGGCGTTTTGGGCCACCAATGACCTGTGGTATGACCCTAG CCGACTTTGAAGAAAAACATTATAAGAGGATCTTTTTTAGAGAGGATCAATCAGAAGGGCCGGTTAGAGA GGAGTACGCAGGGTATCTGCAATATAGAGCCAGAGGGCAATTATTCCTGAGGAATCTCCCGGTGCTAGCA ACAAAAGTCAAGATGCTCCTGGTCGGAAATCTTGGGACGGAGGTGGGAGACTTGGAACACCTTGGCTGGG TCCTTAGGGGGCCTGCCGTTTGCAAGAAGGTTACCGAACATGAGAAATGCACCACATCCATAATGGACAA ATTGACTGCTTTTTTCGGTGTTATGCCAAGGGGCACCACACCTAGAGCCCCTGTGAGATTCCCCACCTCT CTCTTAAAGATAAGAAGGGGGTTGGAAACTGGCTGGGCGTACACACACCAAGGTGGCATTAGTTCAGTGG ACCATGTCACTTGTGGGAAAGACTTGCTGGTATGTGACACTATGGGCCGGACAAGGGTCGTTTGCCAATC AAATAATAAGATGACAGATGAGTCTGAGTATGGAGTTAAAACTGACTCCGGATGCCCGGAAGGAGCTAGG TGTTATGTGTTCAACCCAGAGGCAGTTAACATATCAGGGACTAAAGGAGCCATGGTCCACTTACAAAAAA CTGGAGGAGAATTCACCTGTGTGACAGCATCAGGAACTCCGGCCTTCTTTGATCTCAAGAACCTCAAAGG CTGGTCAGGGCTACCGATATTTGAGGCATCAAGTGGAAGGGTAGTCGGCAGGGTCAAGGTCGGGAAGAAT GAGGACTCTAAACCAACCAAGCTTATGAGTGGAATACAAACAGTCTCCAAAAGTACCACAGACTTGACAG AAATGGTAAAGAAAATAACGACCATGAACAGGGGAGAATTCAGACAAATAACCCTTGCTACAGGTGCCGG AAAAACCACGGAACTCCCTAGGTCAGTCATAGAAGAGATAGGGAGGCATAAGAGAGTCTTGGTCTTGATC CCTCTGAGGGCGGCAGCAGAGTCAGTATACCAATATATGAGACAAAAACATCCAAGCATCGCATTTAACC TGAGGATAGGGGAGATGAAGGAAGGGGACATGGCCACAGGGATAACCTATGCTTCATACGGTTACTTCTG TCAGATGCCACAACCTAAGTTGCGAGCCGCGATGGTTGAGTACTCCTTCATATTTCTTGACGAGTACCAC TGTGCCACCCCAGAACAATTGGCCATCATGGGAAAGATCCACAGATTTTCAGAGAACCTGCGGGTAGTAG CCATGACCGCAACACCAGCAGGCACAGTAACAACCACAGGGCAGAAACACCCTATAGAAGAATTCATAGC CCCAGAAGTGATGAAAGGGGAAGACTTAGGCTCAGAGTACTTGGACATTGCTGGACTAAAGATACCAGTA GAGGAGATGAAGAGCAACATGCTGGTTTTTGTGCCCACTAGGAACATGGCGGTGGAGACAGCAAAGAAAT TGAAAGCTAAGGGTTACAACTCAGGCTACTATTATAGTGGAGAGGATCCATCTAACCTGAGGGTGGTAAC GTCGCAGTCCCCGTACGTGGTGGTGGCAACCAACGCGATAGAATCAGGTGTTACTCTCCCGGACTTGGAT GTGGTTGTCGATACAGGGCTTAAGTGTGAAAAGAGAATACGGCTGTCACCTAAGATGCCCTTCATAGTGA CGGGCCTGAAGAGAATGGCTGTCACGATTGGGGAACAAGCCCAGAGAAGGGGGAGAGTTGGGAGAGTAAA GCCTGGGAGATACTACAGGAGTCAAGAAACTCCCGTTGGTTCTAAAGATTACCATTATGATCTACTGCAA GCACAGAGGTACGGTATTGAAGATGGGATAAACATCACCAAATCCTTTAGAGAGATGAACTATGATTGGA GCCTTTATGAGGAGGACAGTCTGATGATTACACAATTGGAAATCCTCAATAATTTGTTGATATCAGAAGA ACTACCGATGGCAGTAAAAAATATAATGGCCAGGACTGACCACCCAGAACCAATTCAGCTGGCGTACAAC AGCTACGAAACACAAGTGCCAGTGCTATTCCCAAAAATAAAGAATGGAGAGGTGACTGATAGTTACGATA ACTATACCTTCCTCAACGCAAGAAAATTGGGGGATGATGTACCCCCTTACGTGTATGCCACAGAGGATGA GGACTTAGCGGTAGAGCTACTGGGCTTAGACTGGCCAGACCCTGGAAACCAAGGAACCGTAGAGGCTGGC AGAGCACTAAAACAAGTAGTTGGTCTATCAACAGCTGAGAATGCCCTGTTAGTAGCCTTATTCGGCTATG TAGGATATCAGGCACTTTCAAAGAGGCATATACCAGTAGTCACAGATATATATTCAATTGAAGATCACAG GTTGGAAGACACCACACACCTACAGTACGCCCCAAATGCTATCAAGACGGAGGGGAAGGAGACAGAATTG AAGGAGCTAGCCCAGGGGGATGTGCAGAGATGTGTGGAAGCTATGACCAATTATGCAAGAGAGGGTATCC AATTCATGAAGTCTCAGGCACTGAAGGTGAAAGAAACCCCCACTTACAAAGAGACAATGAACACTGTGAC TGACTATGTAAAGAAATTCATGGAGGCGCTGGCAGACAGTAAAGAAGACATCTTAAGATATGGGTTGTGG GGGACGCACACAGCCTTATATAAGAGCATCAGTGCCAGGCTTGGGAGTGAGACTGCGTTCGCTACCCTGG TCGTGAAGTGGCTGGCATTTGGGGGGGAATCAATAGCAGACCATGTCAAACAAGCGGCCACAGACTTGGT CGTCTACTATATCATCAACAGACCTCAGTTCCCAGGAGACACAGAGACACAACAGGAAGGAAGGAAATTT GTGGCCAGCCTACTGGTCTCAGCTCTAGCTACTTACACATACAAAAGCTGGAATTACAATAATCTGTCCA AGATAGTTGAACCGGCTTTGGCCACTCTGCCCTATGCCGCCACAGCTCTCAAACTATTCGCCCCCACTCG ATTGGAGAGCGTTGTCATATTGAGTACCGCAATCTACAAGACCTACCTATCAATCAGGCGCGGAAAAAGC GATGGTTTGCTAGGCACAGGGGTTAGTGCGGCTATGGAGATCATGTCACAAAATCCAGTATCCGTGGGCA TAGCAGTCATGCTAGGGGTAGGGGCCGTGGCAGCCCACAATGCAATCGAGGCCAGTGAGCAGAAGAGAAC ACTACTCATGAAAGTTTTTGTAAAGAACTTCTTGGACCAAGCAGCCACTGATGAATTAGTCAAGGAGAGT CCTGAGAAAATAATAATGGCTTTGTTTGAAGCAGTGCAGACAGTCGGTAACCCTCTTAGACTAGTATACC ACCTTTATGGAGTTTTCTATAAGGGGTGGGAGGCAAAAGAGTTGGCCCAAAGGACAGCCGGTAGGAACCT TTTCACTTTGATAATGTTCGAGGCTGTGGAACTACTGGGAGTAGATAGTGAAGGAAAGATCCGCCAGCTA TCAAGTAATTACATACTAGAGCTCCTGTATAAGTTCCGTGACAGTATCAAGTCTAGCGTGAGGGAGATGG CAATCAGCTGGGCCCCTGCCCCTTTCAGCTGTGATTGGACACCGACGGATGACAGAATAGGGCTCCCCCA AGACAATTTCCTCCAAGTGGAGACGAAATGCCCCTGTGGTTACAAGATGAAGGCAGTTAAGAATTGTGCT GGAGAGCTGAGACTCTTGGAGGAGGAAGGCTCATTTCTCTGCAGAAATAAATTCGGGAGAGGTTCACGGA ACTACAGGGTGACAAAATACTATGATGACAATCTATCAGAAATAAAGCCAGTGATAAGAATGGAAGGGCA TGTGGAACTCTACTACAAGGGAGCCACCATCAAACTGGACTTCAACAACAGTAAAACAATACTGGCAACC GATAAATGGGAGATTGATCACTCCACTCTGGTCAGGGTGCTCAAGAGGCACACAGGGGCTGGATATCATG GGGCATACCTGGGCGAGAAACCGAACTACAAACATCTGATAGAGAGGGACTGTGCAACCATCACCAAAGA TAAGGTTTGTTTTCTCAAAATGAAGAGAGGGTGTGCATTTACTTATGACTTATCCCTTCACAACCTTACC CGACTGATTGAATTGGTACACAAGAATAACTTGGAAGACAAAGAGATTCCTGCTGTTACGGTTACAACCT GGCTGGCTTACACGTTTGTAAATGAAGATATAGGGACCATAAAACCAGCCTTCGGGGAGAAAGTAACACC GGAGATGCAGGAGGAAATAACCTTGCAGCCTGCTGTAGTGGTGGATACAACTGACGTGACCGTGACTGTG GTAGGGGAAGCCCCTACTATGACTACAGGGGAGACTCCGACAGCGTTCACCAGCTCAGGTTCAGACCCGA AAGGCCAACAAGTTTTAAAACTGGGGGTAGGTGAAGGCCAATACCCCGGGACTAATCCACAGAGGGCAAG CCTGCACGAAGCCATACAAGGTGCAGATGAGAGACCCTCGGTGCTGATATTAGGGTCTGATAAAGCCACC TCTAATAGAGTGAAAACTGCAAAGAATGTAAAGGTATACAGAGGCAGGGACCCACTAGAAGTGAGAGATA TGATGAGGAGGGGAAAGATCCTGGTCATAGCCCTGTCTAGGGTTGATAATGCTCTATTGAAATTTGTTGA CTACAAAGGCACCTTTCTAACTAGAGAGACCCTAGAGGCATTAAGTTTGGGTAGGCCTAAAAAGAAAAAC ATAACCAAGGCAGAAGCGCAGTGGTTGCTGTGCCTCGAAGACCAAATGGAAGAGCTACCCGATTGGTTCG CAGCCGGGGAACCCATTTTTCTAGAGGCTAACATTAAACATGACAGGTACCATCTGGTGGGGGATATAGC TAATATCAAGGAAAAAGCCAAACAGTTGGGAGCTACAGACTCCACAAAGATATCTAAGGAGGTTGGTGCA AAAGTGTATTCTATGAAACTGAGTAATTGGGTGATGCAAGAAGAAAATAAACAGGGCAACCTGACCCCCT TGTTTGAAGAGCTCCTGCAACAGTGTCCACCCGGAGGCCAGAACAAAACTGCACATATGGTCTCTGCTTA CCAACTAGCTCAAGGGAACTGGATGCCAACCAGCTGCCATGTTTTTATGGGGACCATATCTGCCAGGAGG ACCAAGACCCATCCATATGAAGCATACGTCAAGTTAAGGGAGTTGGTAGAGGAACACAAGATGAAAACAT TGTGTCCTGGATCAAGCCTGGGTAAGCACAACGAATGGATAATTGGTAAAATCAAATACCAGGGAAACCT GAGGACCAAACACATGTTGAACCCCGGCAAGGTGGCAGAGCAACTGTGCAGAGAGGGACACAGACACAAT GTGTATAACAAGACAATAGGCTCAGTAATGACAGCTACTGGTATCAGGTTGGAGAAGTTGCCCGTGGTTA GGGCCCAGACAGACACAACCAACTTCCACCAAGCAATAAGGGATAAGATAGACAAGGAAGAGAACCTACA AACCCCGGGTTTACATAAGAAACTAATGGAAGTTTTCAATGCATTGAAACGACCCGAGTTAGAGTCCTCC TACGATGCCGTGGAATGGGAGGAACTGGAGAGAGGAATAAACAGGAAGGGTGCTGCTGGTTTCTTTGAAC GCAAAAATATAGGGGAAATATTGGATTCAGAGAAAAACAAAGTCGAAGAGATTATTGACAATCTGAAAAA AGGCAGAAACATCAAATACTATGAAACCGCGATCCCAAAGAATGAGAAGAGGGACGTCAATGATGACTGG ACTGCTGGTGACTTCGTGGAAGAGAAGAAACCCAGAGTCATACAATACCCTGAAGCAAAAACAAGGCTGG CCATCACCAAGGTGATGTATAAGTGGGTGAAGCAGAAGCCAGTAGTTATACCCGGGTATGAAGGGAAGAC ACCTCTATTCCAAATTTTTGACAAAGTAAAGAAGGAATGGGATCAATTCCAAAATCCAGTGGCAGTGAGT TTTGACACTAAGGCGTGGGACACCCAGGTAACCACAAAAGATTTGGAGTTGATAAAGGACATACAAAAGT ACTATTTCAAGAAGAAATGGCATAAATTTATTGACACCCTGACCATGCACATGTCAGAAGTACCCGTAAT CAGTGCTGATGGGGAAGTATACATAAGGAAAGGGCAAAGAGGCAGTGGACAACCTGACACAAGCGCAGGC AATAGCATGCTAAATGTGTTAACAATGATTTACGCCTTCTGCGAGGCCACGGGAGTACCCTACAAGAGCT TCGACAGGGTGGCAAAAATTCATGTGTGTGGGGATGATGGTTTCCTGATCACAGAAAGAGCTCTCGGTGA GAAATTCGCGAGTAAGGGAGTCCAGATCCTATATGAAGCTGGGAAGCCCCAGAAGATCACTGAAGGGGAC AAGATGAAAGTGGCCTACCAATTTGATGATATTGAGTTTTGCTCCCATACACCAATACAAGTAAGGTGGT CAGATAACACTTCTAGTTACATGCCGGGGAGAAATACAACCACAATCCTGGCTAAAATGGCCACAAGGTT AGATTCCAGTGGTGAGAGGGGTACCATAGCATATGAGAAAGCAGTAGCATTCAGCTTCCTGCTGATGTAC TCCTGGAACCCACTAATCAGAAGGATCTGCTTACTGGTGCTATCAACTGAACTGCAAGTGAAACCAGGGA AGTCAACCACTTACTACTATGAAGGGGACCCGATATCTGCCTACAAGGAAGTCATCGGCCACAATCTTTT TGATCTTAAGAGAACAAGCTTCGAGAAGCTGGCCAAGTTAAATCTCAGCATGTCAGTACTCGGAGCCTGG ACTAGACACACCAGTAAAAGACTACTACAAGACTGTGTCAATGTGGGTGTTAAAGAGGGCAACTGGCTAG TTAATGCAGATAGACTAGTAAGTAGCAAGACTGGAAATAGGTACATACCCGGAGAGGGCCACACCCTGCA AGGGAGACATTATGAAGAACTGGTGTTGGCAAGAAAACAGATCAACAACTTTCAAGGGACCGACAGGTAC AATCTAGGCCCAATAGTCAATATGGTGTTAAGGAGGCTGAGAGTCATGATGATGACCCTGATAGGGAGAG GGGTATG >NP_075354.1 polyprotein [Classical swine fever virus] MELNHFELLYKTNKQKPMGVEEPVYDATGRPLFGDPSEVHPQSTLKLPHDRGRGNIKTTLKNLPRKGDCR SGNHLGPVSGIYVKPGPVFYQDYMGPVYHRAPLEFFNEAQFCEVTKRIGRVTGSDGKLYHIYVCIDGCIL LKLAKRDEPRTLKWIRNFTDCPLWVTSCSDDGASGSKEKKPDRINKGKLKIAPKEHEKDSRTKPPDATIV VEGVKYQVKKKGKVKGKSTQDGLYHNKNKPPESRKKLEKALLAWAVIAIMLYQPVEAENITQWNLSDNGT NGIQHAMYLRGVSRSLHGIWPEKICKGVPTYLATDTELKEIQGMMDASEGTNYTCCKLQRHEWNKHGWCN WYNIDPWIQLMNRTQANLAEGPPAKECAVTCRYDKDADINVVTQARNRPTTLTGCKKGKNFSFAGTVIEG PCNFNVSVEDILYGDHECGSLLQDTALYLVDGMTNTIENARQGAARVTSWLGRQLSTAGKRLEGRSKTWF GAYALSPYCNVTSKIGYIWYTNNCTPACLPKNTKIIGPGKFDTNAEDGKILHEMGGHLSEFLLLSLVVLS DFAPETASALYLILHYMIPQSHEEPEGCDTNQLNLTVELRTEDVIPSSVWNVGKYVCVRPDWWPYETKVA LLFEEAGQVVKLALRALRDLTRVWNSASTTAFLICLIKVLRGQIVQGVIWLLLVTGAQGRLACKEDYRYA ISSTNEIGLLGAEGLTTTWKEYNHDLQLNDGTVKAICVAGSFKVIALNVVSRRYLASLHKEASLTSVTFE LLFDGTNPSTEEMGDDFGFGLCPFDTSPVVKGKYNTTLLNGSAFYLVCPIGWTGVIECTAVSPTTLRTEV VKTFRRDKPFPHRMDCATTTVENGDLFYCKLGGNWTCVKGEPVVYTGGLVKQCRWCGFDFNEPDGLPHYP IGKCILVNETGYRIVDSTDCNRDGVVISTDGSHECLIGNTTVKVHASDERLGPMPCRPKEIVSSAGPVRK TSCTFNYAKTLKNKYYEPRDSYFQQYMLKGEYQYWFDLDVTDRHSDYFAEFVVLVVVALLGGRYVLWLIV TYIVLTEQLAAGLPLGQGEVVLIGNLITHTDIEVVVYFLLLYLVMRDEPIKKWILLLFHAMTNNPVKTIT VALLMVSGVAKGGKIDGGWQRLPETSFDIQLALTVIVVAVMLLAKRDPTTVPLVITVATLRTAKMTNGLS TDIAIATVSTALLTWTYISDYYRYKTWLQYLISTVTGIFLIRVLKGIGELDLHTPTLPSYRPLFFILVYL ISTAVVTRWNLDIAGLLLQCVPTLLMVFTMWADILTLILILPTYELTKLYYLKEVKTGAEKGWLWKTNFK RVNDIYEVDQSGEGVYLFPSKQKTSSITGTMLPLIKAILISCISNKWQFIYLLYLIFEVSYYLHKKIIDE IAGGTNFISRLVAALIEANWAFDNEEVRGLKKFFLLSSRVKELIIKHKVRNEVMVHWFGDEEVYGMPKLV GLVKAATLSKNKHCILCTVCEDREWRGETCPKCGRFGPPMTCGMTLADFEEKHYKRIFFREDQSEGPVRE EYAGYLQYRARGQLFLRNLPVLATKVKMLLVGNLGTEVGDLEHLGWVLRGPAVCKKVTEHEKCTTSIMDK LTAFFGVMPRGTTPRAPVRFPTSLLKIRRGLETGWAYTHQGGISSVDHVTCGKDLLVCDTMGRTRVVCQS NNKMTDESEYGVKTDSGCPEGARCYVFNPEAVNISGTKGAMVHLQKTGGEFTCVTASGTPAFFDLKNLKG WSGLPIFEASSGRVVGRVKVGKNEDSKPTKLMSGIQTVSKSTTDLTEMVKKITTMNRGEFRQITLATGAG KTTELPRSVIEEIGRHKRVLVLIPLRAAAESVYQYMRQKHPSIAFNLRIGEMKEGDMATGITYASYGYFC QMPQPKLRAAMVEYSFIFLDEYHCATPEQLAIMGKIHRFSENLRVVAMTATPAGTVTTTGQKHPIEEFIA PEVMKGEDLGSEYLDIAGLKIPVEEMKSNMLVFVPTRNMAVETAKKLKAKGYNSGYYYSGEDPSNLRVVT SQSPYVVVATNAIESGVTLPDLDVVVDTGLKCEKRIRLSPKMPFIVTGLKRMAVTIGEQAQRRGRVGRVK PGRYYRSQETPVGSKDYHYDLLQAQRYGIEDGINITKSFREMNYDWSLYEEDSLMITQLEILNNLLISEE LPMAVKNIMARTDHPEPIQLAYNSYETQVPVLFPKIKNGEVTDSYDNYTFLNARKLGDDVPPYVYATEDE DLAVELLGLDWPDPGNQGTVEAGRALKQVVGLSTAENALLVALFGYVGYQALSKRHIPVVTDIYSIEDHR LEDTTHLQYAPNAIKTEGKETELKELAQGDVQRCVEAMTNYAREGIQFMKSQALKVKETPTYKETMNTVT DYVKKFMEALADSKEDILRYGLWGTHTALYKSISARLGSETAFATLVVKWLAFGGESIADHVKQAATDLV VYYIINRPQFPGDTETQQEGRKFVASLLVSALATYTYKSWNYNNLSKIVEPALATLPYAATALKLFAPTR LESVVILSTAIYKTYLSIRRGKSDGLLGTGVSAAMEIMSQNPVSVGIAVMLGVGAVAAHNAIEASEQKRT LLMKVFVKNFLDQAATDELVKESPEKIIMALFEAVQTVGNPLRLVYHLYGVFYKGWEAKELAQRTAGRNL FTLIMFEAVELLGVDSEGKIRQLSSNYILELLYKFRDSIKSSVREMAISWAPAPFSCDWTPTDDRIGLPQ DNFLQVETKCPCGYKMKAVKNCAGELRLLEEEGSFLCRNKFGRGSRNYRVTKYYDDNLSEIKPVIRMEGH VELYYKGATIKLDFNNSKTILATDKWEIDHSTLVRVLKRHTGAGYHGAYLGEKPNYKHLIERDCATITKD KVCFLKMKRGCAFTYDLSLHNLTRLIELVHKNNLEDKEIPAVTVTTWLAYTFVNEDIGTIKPAFGEKVTP EMQEEITLQPAVVVDTTDVTVTVVGEAPTMTTGETPTAFTSSGSDPKGQQVLKLGVGEGQYPGTNPQRAS LHEAIQGADERPSVLILGSDKATSNRVKTAKNVKVYRGRDPLEVRDMMRRGKILVIALSRVDNALLKFVD YKGTFLTRETLEALSLGRPKKKNITKAEAQWLLCLEDQMEELPDWFAAGEPIFLEANIKHDRYHLVGDIA NIKEKAKQLGATDSTKISKEVGAKVYSMKLSNWVMQEENKQGNLTPLFEELLQQCPPGGQNKTAHMVSAY QLAQGNWMPTSCHVFMGTISARRTKTHPYEAYVKLRELVEEHKMKTLCPGSSLGKHNEWIIGKIKYQGNL RTKHMLNPGKVAEQLCREGHRHNVYNKTIGSVMTATGIRLEKLPVVRAQTDTTNFHQAIRDKIDKEENLQ TPGLHKKLMEVFNALKRPELESSYDAVEWEELERGINRKGAAGFFERKNIGEILDSEKNKVEEIIDNLKK GRNIKYYETAIPKNEKRDVNDDWTAGDFVEEKKPRVIQYPEAKTRLAITKVMYKWVKQKPVVIPGYEGKT PLFQIFDKVKKEWDQFQNPVAVSFDTKAWDTQVTTKDLELIKDIQKYYFKKKWHKFIDTLTMHMSEVPVI SADGEVYIRKGQRGSGQPDTSAGNSMLNVLTMIYAFCEATGVPYKSFDRVAKIHVCGDDGFLITERALGE KFASKGVQILYEAGKPQKITEGDKMKVAYQFDDIEFCSHTPIQVRWSDNTSSYMPGRNTTTILAKMATRL DSSGERGTIAYEKAVAFSFLLMYSWNPLIRRICLLVLSTELQVKPGKSTTYYYEGDPISAYKEVIGHNLF DLKRTSFEKLAKLNLSMSVLGAWTRHTSKRLLQDCVNVGVKEGNWLVNADRLVSSKTGNRYIPGEGHTLQ GRHYEELVLARKQINNFQGTDRYNLGPIVNMVLRRLRVMMMTLIGRGV Virmugen A E2 mutant in Classical swine fever virus is attenuated and provides protection in pigs after oronasal vaccination (not intradermal vaccination) against lethal challenge dose of CSFV strain Eystrup [Ref1976:Maurer et al., 2005]. Protective antigen gp55 VO_0010899 1763691 CDD:292945 11096 ? glycoprotein 6.6 38891.77 452 Pestivirus envelope glycoprotein E2; pfam16329 >AAB39835.1 glycoprotein, partial [Classical swine fever virus] VQGVVWLLLVTGAQGRLACKEDYRYAISSTDEIGLLGAGGLTTTWKEYNHDLQLNDGTVKASCVAGSFKV TALNVVSRRYLASLHKKALPISVTFELLFDGTNPSTEEMEDDLRSGLCPFDTSPVVKGKYNTTLLNGSAF YLVCPIGWTGVIECTAVSPTTLRTEVVKTFRRDKPFPHRMDCVTTTVENEDLFYCKLGGNWTCVKGEPVV YTGGVVKQCRWCGFDFDGPDGLPHYPIGKCILANETGYRIVDSTDCNRDGVVISTEGSHECLIGNTAVKV HASDERLGPMPCRPKEIVSSAGPVMKTSCTFNYTKTLKNRYYEPRDSYFRQYMLKGEYQYWFDLDATDRH SDYFAEFVVLVVVALLRGRYVLWLIVTYAVL Protective antigen Intramuscular vaccination of pigs with immunoaffinity-purified E1 (gp55) in a double water-oil emulsion elicited high titers of neutralizing antibodies between 2 and 4 weeks after vaccination at the lowest dose tested (20 micrograms). The vaccinated pigs were completely protected against intranasal challenge with 100 50% lethal doses of HCV (Classical swine fever virus) strain Brescia [Ref1066:Hulst et al., 1993]. Fernandez-Sainz et al., 2009 journal Fernandez-Sainz I, Holinka LG, Gavrilov BK, Prarat MV, Gladue D, Lu Z, Jia W, Risatti GR, Borca MV 2009 386 1 210-216 Virology Hammond et al., 2001 journal Hammond JM, Jansen ES, Morrissy CJ, Goff WV, Meehan GC, Williamson MM, Lenghaus C, Sproat KW, Andrew ME, Coupar BE, Johnson MA 2001 80 2 101-119 Veterinary microbiology Hulst et al., 1993 journal Hulst MM, Westra DF, Wensvoort G, Moormann RJ 1993 67 9 5435-5442 Journal of virology König et al., 1995 journal König M, Lengsfeld T, Pauly T, Stark R, Thiel HJ 1995 69 10 6479-6486 Journal of virology Lin et al., 2009 journal Lin GJ, Liu TY, Tseng YY, Chen ZW, You CC, Hsuan SL, Chien MS, Huang C 2009 139 3-4 369-374 Veterinary microbiology Maurer et al., 2005 journal Maurer R, Stettler P, Ruggli N, Hofmann MA, Tratschin JD 2005 23 25 3318-3328 Vaccine Moormann et al., 2000 journal Moormann RJ, Bouma A, Kramps JA, Terpstra C, De Smit HJ 2000 73 2-3 209-219 Veterinary microbiology Sainz et al., 2008 journal Sainz IF, Holinka LG, Lu Z, Risatti GR, Borca MV 2008 370 1 122-129 Virology Sun et al., 2013 journal Sun Y, Tian DY, Li S, Meng QL, Zhao BB, Li Y, Li D, Ling LJ, Liao YJ, Qiu HJ 2013 31 3 538-544 Vaccine Tarradas et al., 2011 journal Tarradas J, Álvarez B, Fraile L, Rosell R, Muñoz M, Galindo-Cardiel I, Domingo M, Dominguez J, Ezquerra A, Sobrino F, Ganges L 2011 142 3-4 243-251 Veterinary immunology and immunopathology van, 2003 journal van Aarle P 2003 114 193-200 Developments in biologicals Voigt et al., 2007 journal Voigt H, Merant C, Wienhold D, Braun A, Hutet E, Le Potier MF, Saalmüller A, Pfaff E, Büttner M 2007 25 31 5915-5926 Vaccine Wan et al., 2010 journal Wan C, Yi L, Yang Z, Yang J, Shao H, Zhang C, Pan Z 2010 137 1-2 47-53 Veterinary immunology and immunopathology Wang et al., 2008 journal Wang YH, Li PH, Zhang MT, Zhang YM 2008 24 1 59-63 Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] Wiki: Classical swine fever website http://en.wikipedia.org/wiki/Classical_swine_fever_virus