Mycobacterium tuberculosis 1773 Mouse and guinea pig models have been used extensively to understand the host and pathogen-specific factors controlling pathogenesis of tuberculosis. Transmission of M. tuberculosis via intravenous route in murine models is followed by initial bacterial replication and subsequent macrophage activation via IL-6, IL-12, and TNF-mediated mechanisms. Bacterial load is significantly reduced at 2 weeks post-infection in liver and spleen. After load reduction, selected lymphocytes are activated which can then induce macrophages to begin producing nitric oxide via the iNOS pathway. Bacteria which survive the initial clearance by the host enter a viable yet non-replicating persistence stage. Factors such as immune suppression from aging, iNOS inhibition, and corticosteroid therapy can shift the dormant bacilli back into a replicative state. As an example of preferred host niche, transmission via inhalation will sometimes result in bacilli establishing infection in macrophage phagosomes in the lung. These macrophages become lodged in the calcified tubercles, and can later initiate formation of granulomas through a CD4-mediated delayed-type hypersensitivity reaction. Each granuloma can remain dormant, reduce in size and possibly disappear, initiate progressive interstitial fibrosis in lung tissue, and/or necrotize (Ernst et al., 2007). Similar mechanisms are observed in liver tissue (Ducati et al., 2006). Numerous pathogen- and host-specific factors related to pathogenesis of tuberculosis are potential vaccine targets. For example, the Bacille-Calmette Guiren (BCG) vaccine was obtained from an M. bovine strain which lost its virulence after 39 passages (Ducati et al., 2006). Evidence from subtractive hybridization experiments, along with whole-genome microarrays and BAC arrays and other experimental evidence, suggests that the RD1 locus is the main virulence feature that is absent from 13 strains of avirulent BCG yet present in hundreds of other M. tuberculosis strains (Ernst et al., 2007). RD1 is related to the regulation of the early secreted antigen 6 kDa [ESAT-6] secretion system 1[ESX-1], an important pathway which is linked to the recruitment and infection of macrophages to initially infected sites and subsequent disease propagation (Ernst et al., 2007). Tuberculosis Although it is known that IFN-gamma is essential for protective immunity, animal and human studies have found that IFN-gamma alone is not sufficient for the prevention of TB disease. There is evidence that IL-23, a recently described member of the IL-12 family of cytokines, is important in the immuno-pathogenesis of TB. There is also evidence that regulatory T cells (Treg) are present in TB disease and that Treg may suppress effector T cell responses (Fletcher, 2007). New vaccines seek to target specific CD1, CD4+, and CD8+ T and B cell responses to assist in eradicating the disease via improved clearance of infected cells and bacterial loads. Host ranges extend to many animals, including human, mouse, guinea pig, rabbit, and cattle. The most predominant animal used to study M. tuberculosis for virulence assessment and vaccine development is likely the mouse, which include C57BL/6 and BALB/c strains, mainly due to cost effectiveness for long-term vaccination/challenge studies. Dunkin Hartley guinea pigs are often used in M. tuberculosis studies as a close model for the overall pathogenesis in humans. At the end of 19th century, Robert Koch discovered that Mycobacterium tuberculosis was the causative agent of tuberculosis (TB). TB is a major cause of morbidity and mortality throughout the world despite more than a half century of widespread vaccination, claiming millions of lives every year. The extraordinary high prevalence of the latent infection [estimated at one-third of the world's population] is one of the main factors contributing to the high incidence of active tuberculosis. The other factor is convergence of the TB and HIV epidemics. With HIV/AIDS fueling the TB crisis, the WHO has estimated that more than 1 billion people will be infected with TB bacilli in the next 20 years (Gupta et al., 2007). M. tuberculosis are Gram-positive bacilli lacking both flagella and capsules. M. tuberculosis is preferentially established in the pulmonary system in a dormant state after initial infection via aerosol transmission. The M. tuberculosis complex, a group of Mycobacterium including M. tuberculosis, M. bovis, M. bovis BCG, M. africanum, and M. microti, are tuberculosis-inducing bacteria which have low horizontal gene transfer and variable levels of virulence (Ducati et al., 2006). Baboon Papio cynocephalus 9556 Bank vole Clethrionomys glareolus 447135 Bear Ursus americanus 9643 Birds Passeroidea 175121 Brown Trout Salmo trutta 8032 Buffalo Bison bison 9901 Carnivores Vulpes 9625 Cat Felis catus 9685 Catfishes Siluriformes 7995 Cattle Bos taurus 9913 Chicken Gallus gallus 9031 Chimpanzee Pan troglodytes 9598 chinchillas Chinchillidae 10150 Copper Pheasant Syrmaticus soemmerringii 9067 Deer Cervus elaphus 9860 Deer mouse Peromyscus maniculatus 10042 Dog Canis familiaris 9615 Ducks Anas 8835 Ferret Mustela putorius furo 9669 Fish Hyperotreti 117565 Gerbil Gerbillina 10045 Goat Capra hircus 9925 Gray wolf Canis lupus 9612 Guinea pig Cavia porcellus 10141 Hamster Mesocricetus auratus 10036 Horse Equus caballus 9796 Human Homo sapiens 9606 Macaque Macaca fascicularis 9541 Mongolian Gerbil Meriones unguiculatus 10047 Monkey Platyrrhini 9479 Mouse Mus musculus 10090 None None Parrot Psittacidae 9224 Pig Sus scrofa 9823 Rabbit Oryctolagus cuniculus 9986 Rainbow trout Oncorhynchus mykiss 8022 Rat Rattus 10114 Raven Corvus corax 56781 sei whale Balaenoptera borealis 9768 Sheep Ovis aries 9940 Squirrel Spermophilus richardsonii 37591 Tree shrew Tupaiidae 9393 Trouts, salmons & chars Salmoninae 504568 Turkey Meleagris gallopavo 9103 Vole Microtus ochrogaster 79684 Water buffalo Bubalus bubalis 391902 A986 + MPL/QS21 Subunit vaccine Research subcutaneous injection A986 + MPL/QS21 is a multistage recombinant fusion protein tuberculosis vaccine composed of Ag85A, Rv2028c, Rv2029c, and Rv3126c, formulated with MPL/QS21 adjuvant and administered as a booster following BCG priming to enhance protective immunity against Mycobacterium tuberculosis (Chen et al., 2024). MPL/QS21 is used as the adjuvant (Chen et al., 2024). The A986 fusion protein was recombinantly expressed and purified, then formulated with the MPL/QS21 adjuvant prior to subcutaneous administration as a booster vaccine following BCG priming (Chen et al., 2024). subcutaneous injection A986 is a recombinant multistage fusion protein composed of Ag85A, Rv2028c, Rv2029c, and Rv3126c (Chen et al., 2024). Fusion Protein Use in fusion protein preparation (Chen et al., 2024). Fusion Protein This is use in fusion protein preparation (Chen et al., 2024). Fusion Portein This is use in fusion protein preparation (Chen et al., 2024). Fusion Portein Use in fusion protein preparation (Chen et al., 2024). BALB/c Mice were primed with a single subcutaneous injection of 5 × 10⁶ CFU BCG at week −8, followed by three subcutaneous booster doses of 10 μg A986 formulated with 5 μg MPL/QS21 administered at two-week intervals, and subsequently challenged with aerosolized Mycobacterium tuberculosis H37Rv (Chen et al., 2024). A986 + MPL/QS21, when used as a BCG booster, significantly reduced lung bacterial burden compared with BCG alone, demonstrating enhanced protective efficacy in a mouse model (Chen et al., 2024). Vaccinated mice were challenged with aerosolized Mycobacterium tuberculosis H37Rv at approximately 100 CFU, and bacterial burden was measured in the lungs and spleen (Chen et al., 2024). Vaccination with A986 + MPL/QS21 induced strong Th1-type immune responses characterized by increased IFN-γ, TNF-α, and IL-2 production, enhanced CD4⁺ T-cell activation, and improved memory T-cell formation compared with controls (Chen et al., 2024). AER in PLGA nanoparticles + CpG + MPLA Subunit vaccine Research subcutaneous injection The vaccine includes CpG (a TLR9 agonist) and MPLA (a TLR4 agonist) as immunostimulatory adjuvants (Szachniewicz et al., 2024). The recombinant AER fusion protein was encapsulated within PLGA (poly(D,L-lactic-co-glycolic acid)) nanoparticles together with CpG and MPLA. Each dose contained 8 μg AER, 2.5 μg CpG, and 1 μg MPLA, incorporated into approximately 400 μg PLGA nanoparticles prior to subcutaneous administration (Szachniewicz et al., 2024). subcutaneous injection AER is a recombinant fusion protein composed of Ag85B, ESAT-6, and Rv2034 antigens of Mycobacterium tuberculosis (Szachniewicz et al., 2024). Fusion Protein Use in fusion protein preparation (Szachniewicz et al., 2024). Fusion Protein This is use in fusion protein preparation (Szachniewicz et al., 2024). Fusion Protein This is use in fusion protein preparation (Szachniewicz et al., 2024). Vaccination induced strong Th1-type immunity characterized by increased IFN-γ, TNF-α, and IL-2 production, enhanced multifunctional T-cell responses, and improved memory T-cell formation (Szachniewicz et al., 2024). C57BL/6 Mice were immunized subcutaneously three times at two-week intervals with AER encapsulated in PLGA nanoparticles containing CpG and MPLA (Szachniewicz et al., 2024). Four weeks after the final immunization, mice were intranasally challenged with 10⁵ CFU of Mycobacterium tuberculosis H37Rv, and bacterial burden was assessed six weeks post-infection (Szachniewicz et al., 2024). This vaccine consists of the recombinant AER fusion protein (Ag85B–ESAT-6–Rv2034) encapsulated in PLGA nanoparticles and formulated with CpG and MPLA, inducing strong Th1-biased cellular immunity and protective efficacy against Mycobacterium tuberculosis in a mouse model (Szachniewicz et al., 2024). Ag85B and ESAT-6 fusion protein VO_0000536 Conjugate vaccine 1 25 μg amount of monoposphoryl lipid A (MPL) adjuvant was added to 250μg dimethyl dioctadecylammonium bromide (DDA) emulsifier per 0.01-50μg of vaccine. M tuberculosis Erdamn and H37Rv were grown in media, while BCG Danish 1331 was acquired as a freeze dried-vaccine and replenished (Olsen et al., 2001). Recombinant ESAT-6 was obtained along with short-term culture filtrate. Esat6 and ag85B coding regions were obtained by PCR amplification from M. tuberculosis H37Rv chromosomal DNA using selected primers. PCR products were digested by two restriction endonucleases and cloned into pMCT6 prior to sequencing. The E. coli XL-1 Blue was used to express the His-tagged protein prior to purification, protein anion-exchange chromatography, and dialysis. PCR fragments per gene were joined at a HindIII site, cloned into pMCT6, expressed, and later purified to create a fusion protein and a chimeric plasmid. Two immunodominant antigens, Ag85B and ESAT-6, were used in the analysis. ESAT-6 is a 6-kDA early secretory antigenic target. The two antigens are combined as a fusion protein in the vaccine (Olsen et al., 2001). Fusion protein ESAT6-Ag85B Fusion protein created in E. coli using His-tag cloning system pMCT6. Proteins were affinity purified, separated via ion exchange chromatography, and analyzed with SDS-PAGE and Western blots (Olsen et al., 2001). Fusion protein Ag85B-ESAT6 C57BL/6J (H-2^b), specific pathogen-free female; B6CBAF1 Female mice 6-12 weeks old were housed within a BSL-3 enclosure. Mice were immunized with 0.01-50 μg vaccine emulsed in 250 μg dimethyl dioctadecylammonium bromide (DDA) and 25 μg of monophosphoryl lipid A (MPL) aduvant. Vaccines were injected 3 times subcutaneously on back at 2-week intervals (Olsen et al., 2001). BCG Danish 1331 dose (5x10^4 baccilli/mouse) was administered via subcutaneous injection at base of tail simultaneously with first subunit vaccination. No booster injections were included (Olsen et al., 2001). Mice were sacrificed at either 2 or 6 weeks post-innoculation via i.v. or aerosol routes, respectively prior to organ removal and bacterial enumeration(Olsen et al., 2001). A statistical reduction in the number of bacteria was observed for vaccine doses as low as .01μg. The 0.1-10μg dose range showed a higher level of protection versus the .01μg dose. 50 μg doses resulted in reduced protection in lung and spleen, whereas, 10 μg showed significant levels of protection in spleen. The fusion protein Ag85B-ESAT-6 showed a statistically significant improvement in reduction of bacteria versus the individual Ag85B and ESAT-6 components (Olsen et al., 2001). Both recombinant proteins induced long-lived memory immunity when challenged 10 or 30 weeks post-first vaccination, while BCG showed significantly lower protection levels at the later challenge points(Olsen et al., 2001). Prechallenge immunity was assessed 5 weeks post-first vaccination (Olsen et al., 2001). Mice were challenged at either 10 or 30 weeks post-immunization via aerosol route (100 CFU of M. tuberculosis Erdman per lung) or intravenous route (0.2 ml volume containing 5x10^4 CFU M. tuberculosis H37Rv in PBS) (Olsen et al., 2001). Blood lymphocytes were purified and pooled from eight mice per group and cultured in triplicate as 2x10^5 cells in 200μl of RPMI 1640 with 2-mercaptoethanol, glutamine, penicillin-streptomycin, and 5% v/v fetal calf serum. Mycobacterial antigens administered as 5-1.3μg/ml. Interferon gamma amounts were determined using ELISA (Olsen et al., 2001). Ag85B:HspX:hFcγ1 Subunit vaccine Research subcutaneous injection Adjuvants include DDA and TDB. Mixed at a 5 to 1 ratio (Karbalaei et al., 2024). The vaccine consists of a recombinant protein expressed in Pichia pastoris, purified by Protein A affinity chromatography, and formulated as a protein subunit vaccine(Karbalaei et al., 2024). subcutaneous injection Ag85B:HspX:hFcγ1 Fusion Protein Use in fusion protein preparation(Karbalaei et al., 2024). Fusion Protein Use in fusion protein preparation(Karbalaei et al., 2024). Vaccination induced strong Th1 responses characterized by increased IFN-γ and IL-12 expression, with moderate TGF-β production and no significant IL-4 induction (Karbalaei et al., 2024). C57BL/6 Subcutaneous immunization of mice with Ag85B:HspX:hFcγ1 formulated with DDA/TDB, administered four times at two-week intervals (Karbalaei et al., 2024). AH-PB Subunit vaccine Research Nasal spray The adjuvant is Poly I:C (polyinosinic–polycytidylic acid) (Liang et al., 2022). Fusion genes (Ag85A–HspX) were cloned, expressed recombinantly, and purified. Proteins were formulated with Poly I:C (± β-defensin-5) (Liang et al., 2022). Nasal spray AH fusion proteins genetically linking Ag85A (FbpA) and HspX (Liang et al., 2022). Fusion Protein This is use in fusion protein preparation (Liang et al., 2022). Fusion Protein This is use in fusion protein preparation (Liang et al., 2022). C57BL/6 Mice were immunized intranasally with AH-PBfusion proteins formulated with poly(I:C) in a prime–boost regimen, with booster immunizations administered at defined intervals (Liang et al., 2022). Vaccinated C57BL/6 mice were intranasally challenged with a low dose (~100 CFU) of Mycobacterium bovis, and protection was assessed by bacterial burden and lung pathology (Liang et al., 2022). In a C57BL/6 mouse model, the vaccine induced robust cellular and mucosal immune responses and conferred significant protection against Mycobacterium bovis infection (Liang et al., 2022). AHB-SeNPs Subunit vaccine Research Nasal spray The adjuvant is Selenium nanoparticles (SeNPs) (Ge et al., 2024). Nasal spray AHB fusion protein composed of Ag85A, HspX, and bovine neutrophil β-defensin-5 (B5). Fusion Protein This is use in fusion protein preparation (Ge et al., 2024). Fusion Protein This is use in fusion protein preparation (Ge et al., 2024). Fusion Protein This is use in fusion protein preparation (Ge et al., 2024). C57BL/6 Mice received three intranasal immunizations at three-week intervals. Each dose consisted of 20 µg AHB fusion protein formulated with 20 µg selenium nanoparticles (SeNPs) in PBS (Ge et al., 2024). Vaccinated mice showed significantly reduced bacterial loads in lungs and spleen. Lung inflammation and pathological lesions were markedly decreased compared to controls (Ge et al., 2024). Mice were intranasally challenged with ~100 CFU of Mycobacterium bovis (Beijing strain C68004) three weeks after the final immunization. Bacterial burden and pathology were assessed 4 weeks post-challenge (Ge et al., 2024). In a C57BL/6 mouse model, the vaccine induced robust Th1/Th17 cellular immunity, mucosal antibody responses, and lung-resident memory T cells, resulting in significant protection against Mycobacterium bovis infection (Ge et al., 2024). AR2+DMC Subunit vaccine Research subcutaneous injection The vaccine was formulated with a compound liposomal adjuvant (DMC) composed of DDA (dimethyldioctadecylammonium), CpG oligodeoxynucleotides, and monophosphoryl lipid A (MPLA) (Wang et al., 2022). The AR2 fusion protein gene was cloned into a prokaryotic expression vector, expressed in Escherichia coli BL21 (DE3), purified under denaturing conditions using His-tag affinity chromatography, and treated with an endotoxin removal kit to reduce endotoxin levels below 0.1 EU/mL before formulation with adjuvants (Wang et al., 2022). subcutaneous injection The antigen is a fusion protein AR2, composed of Mycobacterium tuberculosis antigens Ag85B (Rv1886c) and Rv1988 (Wang et al., 2022). Fusion Protein This is used in fusion protein preparation(Wang et al., 2022). Fusion Protein Use in prepare fusion protein(Wang et al., 2022). Immunization with AR2, particularly when combined with the DMC adjuvant, induced strong Th1-biased immune responses characterized by elevated IFN-γ, TNF-α, IL-2, and iNOS expression, increased numbers of IFN-γ-secreting CD4⁺ and CD8⁺ T cells, and higher IgG and IgG2a antibody titers(Wang et al., 2022). C57BL/6 Mice were immunized subcutaneously with AR2-based vaccines every 3 weeks for a total of three doses, using PBS as a negative control and BCG as a positive control, followed by immunological analysis at week 9 (Wang et al., 2022). AR2 enhance Th1-biased immune responses and designed as a booster strategy to improve BCG-induced immunity(Wang et al., 2022). Arabinomannan-tetanus toxoid conjugate VO_0000528 Conjugate vaccine Alum (aluminum hydroxide gel) adjuvant was used initially. Eurocine L3 suspension adjuvant was prepared using 1:1 ratio solid monooleate and oleic acid, Tris buffer with pH adjustment including NaOH, and subsequent sonification. Eurocine L3 emulsion was prepared using 1:1 ratio solid monooleate and oleic acid which were liquified prior to addition of soy bean oil (Hamasur et al., 2003). Arabinomannan (AM) oligosaccharides derived from LAM of Mycobacterium tuberculosis H37Rv were isolated and covalently conjugated to tetanus toxoid (TT) or to short-term culture filtrate proteins (antigen 85B (Ag85B) or a 75kDa protein) from M. tuberculosis strain Harlingen (Hamasur et al., 2003). Arabinomannan (AM) oligosaccharides. They are derived from Lipoarabinomannan (LAM), a carbohydrate antigen expressed on the surface of mycobacteria which contains a manna polysaccharide core attached to a phosphatidyl inositol lipid moiety (Hamasur et al., 2003). The surface carbohydrate was hypothesized to yield CD1-specific immune responses, promote mycobacterial clearance, downregulate T cell proliferation, and interfere with activation of macrophages through interferon gamma intervention (Hamasur et al., 2003). Conjugate protein purified from LAM The 28-kDa AM oligos were pooled and linked to tetanus toxoid (TT) or other M. tuberculosis proteins (Ag85B, 75-kDa protein) with thioether linkage generated by conjugation method. Conjugation consisted of amination of AMO with ammonium chloride and sodium cyanoborohydride, deasalting and treatment with 2-imminothiolane, and end conjugation between thiolated oligsaccharide derivatives with bromoacetylated proteins and tributyl phosphine (Hamasur et al., 2003). Immunization with AMO-TT, AMOs-Ag85B, or AMOs-75kDa conjugate resulted in increased lymphocyte proliferation in spleen when responding to PPD (Hamasur et al., 2003). C57BL/6, 8-10 wk female Mice were injected subcutaneously in flanks with 100μL or intranasal with 5μL/nostril of antigen plus adjuvant. The control BCG vaccination involved subcutaneous injection of 5x10^5 CFU in 100μL PBS in hind flank. Two formulations of the adjuvant were used, such that mice were either treated at day 0 with AMOs-TT conjugate in Eurocine L3 emulsion or suspension, followed by nasal booster at day 21 (Hamasur et al., 2003). All mice started to die after 17 weeks (median time = 36 weeks) (Hamasur et al., 2003). Considerable increases in weight were observed for mice immunized with AMOs-TT conjugate or BCG versus those not vaccinated (Hamasur et al., 2003). Mice immunized with AMOs-TT conjugate and either adjuvant showed longer survival than sham-immunized mice. The emulsion adjuvant showed increased protective efficacy versus the suspension version (Hamasur et al., 2003) Mice were challenged intranasally with 10^5 M. tuberculosis Harlingen on day 24 (vaccination on day 0). C57BL/6, 8-10 wk female Mice were immunized subcutaneously on days 0, 28 with 5 or 25 μg of AMO-Ag85B in 1% alum. Control mice were vaccinated s.c. with live BCG on day 0. BCG-immunized mice exhibited best survival versus mice immunized with the AMO-Ag85B vaccine. However, AMO-Ag85B immunized mice also showed significant improvement in survival versus sham-immunized mice. Reduced weight loss observed for BCG and AMO-Ag85B immunized mice versus shams (Hamasur et al., 2003). Mice were challenged intravenously with 10^5 CFU M. tuberculosis H37Rv on day 60(Hamasur et al., 2003). Dunkin Hartley, female (250-300g) Guinea pigs were immunized s.c. (day 0) and boosted nasally (day 24) with Eurocine L3 emulsion, AMO-TT conjugate, and variable antigen doses and adjuvant concentrations (Hamasur et al., 2003). A protection study included immunization with 15 μg AMO-Ag85B in 10% Eurocine L3 emulsion. Comparison BCG groups were injected s.c. with 5x10^4 CFU live BCG (Hamasur et al., 2003). BCG vaccinated animals showed reduced counts of M. tuberculosis bacilli in lung and spleen samples versus saline controls. The reduced CFUs in spleen samples along with histological sections analyzed with double blind analysis suggested an overall decrease in disease severity in lung and spleen (Hamasur et al., 2003). Five of six animals non-vaccinated and two of six AMO-Ag85B vaccinated were killed before 120 days post-challenge if observed >20% loss of starting weight. Guinea pigs were aerosol challenged with saline suspension containing 10^6 organisms per ml (~10 CFU/lung). LAM alone did not induce LAM IgG antibodies, whereas the three conjugate vaccines induced high IgG antibody titers (Hamasur et al., 2003). White New Zealand female rabbits (2.5-3kg) Rabbits were immunized with LAM (50 μg), AMO-TT (20 μg), AMO-Ag85B (20 μg), or AMO-75kDA (20 μg) conjugates via i.m. injection with 100 μl PBS and 100 μl FIA emulsifier, and later boosted (weeks 2, 4) with same dose of the previous vaccine. No challenge protocol implemented for rabbit immunogenicity study. Purpose of the experiment with rabbits was to determine immunogenicity response to LAM and AMO-protein conjugates. ARM Subunit vaccine Research Intramuscular injection (i.m.) The adjuvant is CpG oligodeoxynucleotide 1826 (CpG ODN 1826) (Hu et al., 2025). The ARM fusion protein was recombinantly expressed and purified, then dissolved in phosphate-buffered saline (PBS) and mixed with CpG ODN 1826 prior to intramuscular immunization (Hu et al., 2025). Intramuscular injection (i.m.) ARM fusion protein composed of Ag85B, Rv2660c, and MPT70 from Mycobacterium tuberculosis (Hu et al., 2025). Fusion Protein This is use in fusion protein preparation (Hu et al., 2025). Fusion Protein This is use in fusion protein preparation (Hu et al., 2025). Fusion Protein This is use in fusion protein preparation (Hu et al., 2025). C57BL/6 Mice were intravenously infected with Mycobacterium tuberculosis H37Ra and, four weeks post-infection, were immunized intramuscularly with the ARM fusion protein formulated in PBS with CpG ODN 1826. A booster immunization was administered two weeks later, and immune responses and bacterial loads were evaluated at multiple time points post-treatment (Hu et al., 2025). ARM vaccination significantly reduced bacterial loads in the lungs and spleen, improved body weight recovery, and alleviated pulmonary pathology compared to PBS-treated controls and BCG-vaccinated mice (Hu et al., 2025). Mice were challenged by intravenous infection with Mycobacterium tuberculosis strain H37Ra prior to vaccination to establish a post-exposure therapeutic model (Hu et al., 2025). ARM is a tuberculosis fusion protein vaccine composed of Ag85B, Rv2660c, and MPT70, evaluated as a therapeutic subunit vaccine in a post-exposure mouse model. The vaccine enhances both cellular and antibody-mediated immunity, leading to improved control of Mycobacterium tuberculosis infection and reduced disease pathology (Hu et al., 2025). attenuated M. bovis strain WAg539 VO_0000543 Live, attenuated vaccine Signature tag morphogenesis was used to generate illegitimate recombinants of M. bovis. Several of the recombinant strains produced protection that was "at least as good as that provided by M. bovis BCG" in guinea pig models (Collins et al., 2005). M. bovis strain and its recombinants were cultured in Middlebrook 7H9 (Difco) liquid medium supplemented with albumin-dextrose complex (Difco), 0.05% Tween 80, and and 0.4% sodium pyruvate and on Middlebrook 7H11 (Difco) solid medium supplemented with albumin-dextrose complex and 0.4% sodium pyruvate. Recombinants were cultured on kanamycin-supplemented medium. M. bovis culture from pig spleens were homogenized, filtered, and further processed (Collins et al., 2005). Forty-five recombinants with unique tags were subcultured separately and pooled together. The forty-five M. bovis recombinants were subcultured separately, and 10^6 CFU of each pool was inoculated subcutaneously into the flank of each guinea pig. Splenic culture were extracted from sacrificed animals and used to isolate 500-2,000 colonies per spleen. Tags identified in both the inoculated and recovered pools were amplified by PCR and processed. M. bovis with gene mutations due to signature-tagged mutigenesis to create attenuated mutants. Ten genes were found to be deleted or interrupted in the 10.1-kb deletion in WAg539, including mmpL4, mmpS4, ufaA1, and sigK, as well as unknown genes (Collins et al., 2005). Dunkin-Hartley Groups of six guinea pigs were vaccinated with 10^5 CFU of one of the avirulent recombinant strains or BCG. Six animals were not vaccinated (control group) (Collins et al., 2005). Four mutants which did not induce any visible lesion were tested for vaccine efficacy. In the case of three mutants, animals were sacrificed 5 weeks post-challenge. The final mutant, WAg585 were sacrificed at 8 weeks post-challenge. Mutant WAg539 gave better, though not significantly different, protection than BCG vaccine, as well as the best vaccination of the four mutants (Collins et al., 2005). Guinea pigs were challenged by aerosol with 2 to 10 CFU of wild-type M. bovis eight weeks post-vaccination (Collins et al., 2005). Animals were sacrificed and autopsied 5 weeks after challenge to enable the following analyses: weight, gross pathology, mycobacterial culturing and enumeration in splenic and lung tissues, and statistical analysis of macroscopic lesion count and other quantifiable measurements (Collins et al., 2005). Bacille Calmette-Guérin (BCG) BCG VO_0000771 Attenuated live vaccine Albert Calmette and Camille Guerin, two French scientists, made an attenuated strain from another mycobacterium (M. bovis) by growing it for 13 years on culture. They observed a reduction in the virulence in animals through this period. When infants were given this vaccine, it provided a reduction in mortality by 90%. Since then, Bacille Calmette Guerin [BCG] has become the most widely used vaccine throughout the world. It is estimated that more than 3 billion people have received BCG. The protective efficacy of BCG vaccine against adult pulmonary TB has varied dramatically from 80 to 0% (Gupta et al., 2007). Development of the BCG strain was predated by numerous M. tuberculosis studies from the 1890s-1920s, including (Lote 1889, Strauss 1895, Moriya 1909, Romer 1903, Weber 1912, Binder 1915, and Browning and Gulbransen 1926) in a diverse set of hosts (including mouse, avian, human, bovine, and guinea pig hosts) (SWEDBERG, 1951). Several new subunit booster vaccinations are under development, including Ag85, Mtb72F, HspX and other DosR-controlled gene products, Rv3407 and other Rpf-controlled gene products, and heparin-binding hemagglutinin (HBHA) (Kaufmann, 2005). The original BCG strain lost its virulence after 39 passages by Albert Calmette and Camille Guerin, and was administered to humans i.d. on the 231st passage. The BCG bacteria showed altered colony morphologies and no virulence in experimental animals. BCG has shown a high degree of safety over the years (Ducati et al., 2006). --Calmette et al. used length of survival as a metric for virulence in their 1923 study (Agger et al., 2002). --The increased ability for M. bovis strains to retain virulence was noted by Lange in 1922 (SWEDBERG, 1951). The evaluation of BCG lacks comparability among the vaccination studies due to the use of six different BCG sub-strains [Copenhagen-1331, Pasteur-1173P, Glaxo-1077, Tokyo-172, Russian, Moreau]. The comparative genomic analysis of different BCG strains has shown that there is no uniformity among all presently used vaccines and this genetic variability may contribute to the variation in protective efficacy seen in different geographic regions of the world (Gupta et al., 2007). In one case, Calmette et al. (1923) used decreasing doses (1 mg, .01 mg, and .002 mg) of tubercle bacilli to infect guinea pigs s.c. Bacterial loads increased in response to decreasing dose sizes. Similar results were acquired for later intramuscular and intracerebral infection procedures by other groups (SWEDBERG, 1951) TB Ag-specific mucosal, humoral and cellular immune response via T cell proliferation and production (Wang et al., 2009). SL(E6-85B) vaccine, when combined with BCG vaccination, induced the strongest TB Ag-specific mucosal, humoral, and cellular immune responses comprised of increased proliferation of T cells, IFN-gamma expression, granzyme B production (Wang et al., 2009). The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced significant TB Ag-specific IFN-gamma immune responses in splenocytes. Splenocytes were stimulated with the Ag85B protein 2 weeks after the final immunization (Wang et al., 2009). Patients with the NRAMP1 D543N G:G genotype and allele 3 (GT)n polymorphism had decreased recurrence time after BCG therapy. (Chiong et al., 2011) DNA was obtained from the peripheral blood of 99 NMIBC patients who were prospectively randomized to receive postresection intravesical BCG or BCG with interferon alpha. (Chiong et al., 2011) Polymorphisms of the SLC11A1 genes may be associated with recurrence of BCa after BCG immunotherapy. (Chiong et al., 2011) Polymorphisms of the GPX1 gene may be associated with recurrence of BCa after BCG immunotherapy. (Chiong et al., 2011) Increased production of TH1-type T cell cytokines (Randhawa et al., 2011). Intradermally vaccinated the infants within 48 hours of birth. Examination of whole blood stimulated ex vivo with 1.2×106 CFU BCG 10 weeks after newborn BCG vaccination of South African infants (Randhawa et al., 2011). After stimulation with TLR1 lipopeptide ligands, PBMCs from TLR1-deficient, BCG-vaccinated individuals secreted lower amounts of IL-6 and IL-10 compared to those with responsive TLR1 genotypes (Randhawa et al., 2011). After stimulation with TLR6 lipopeptide ligands, PBMCs from TLR6-deficient, BCG-vaccinated individuals secreted lower amounts of IL-6 and IL-10 compared to those with responsive TLR6 genotypes (Randhawa et al., 2011). Increased IFNG production associated with TLR1/6 presence 10 weeks post BCG vaccination (Randhawa et al., 2011). Increased IL2 production associated with TLR1/6 presence 10 weeks post BCG vaccination (Randhawa et al., 2011). IL6 expression post vaccination expressed in higher amounts in presence of TLR1/6 (Randhawa et al., 2011). IL10 expression post vaccination increased in presence of TLR1/6 (Randhawa et al., 2011). Immunization with BCG[pMV361] (BCG with empty pMV361) vaccine induced significant up regulation of IFN-gamma in mice 45 days after immunization as compared to non-immunized mice. Cytokines were measured in lymphocytes from the spleen (Singh et al., 2011). Immunization with BCG[pMV361] (BCG with empty pMV361) vaccine induced significant up regulation of IL-2 in mice 45 days after immunization as compared to non-immunized mice. Cytokines were measured in lymphocytes from the spleen (Singh et al., 2011). Immunization with BCG[pMV361] (BCG with empty pMV361) vaccine induced significant up regulation of IL-6 in mice 45 days after immunization as compared to non-immunized mice. Cytokines were measured in lymphocytes from the spleen (Singh et al., 2011). Immunization with BCG[pMV361] (BCG with empty pMV361) vaccine induced significant up regulation of TNF-alpha in mice 45 days after immunization as compared to non-immunized mice. Cytokines were measured in lymphocytes from the spleen (Singh et al., 2011). A single immunization of mice with Lactoferrin as an adjunct adjuvant resulted in amplified splenocyte proliferative response to heat-killed BCG, and elevated IL-12(p40) production with increased relative ratios of IL-12/IL-10. Furthermore, splenocyte recall response to HK-BCG was augmented for proinflammatory mediators, TNF-alpha, IL-1beta, and IL-6, approaching responses generated to complete Freund's adjuvant (CFA) immunized controls (Hwang et al., 2005). C57BL/6 Ten to twelve female C57BL/6 mice were immunized per group with 100 μl of the formulation, once, subcutaneously (s.c.) at the base of the tail. BCG and Lactoferrin were emulsified with Freund's adjuvant in a 1:1 ratio (Hwang et al., 2005). All immunization groups showed significant reduction in lung organism load, decreased bacterial load in the spleen (Hwang et al., 2005). Fourteen days post-immunization, four mice from each group were aerosol challenged with Erdman MTB (Hwang et al., 2005). Lactoferrin can act as an adjunct adjuvant to augment cellular immunity and boost BCG efficacy for protection against subsequent challenge with virulent MTB. Augmented responses were found for IL-12, TNF-alpha, IL-1beta, and IL-6 (Hwang et al., 2005). A single administration of BCG in IFA and Lactoferrin resulted in significantly increased production of IL-12 in the splenic recall assay (Hwang et al., 2005). In concert with the increased stimulation index, there was significant production of all three proinflammatory mediators (TNF-alpha, IL-1beta, and IL-6) in the Lactoferrin immunization group compared to both the non-immunized and IFA immunized groups (Hwang et al., 2005). In concert with the increased stimulation index, there was significant production of all three proinflammatory mediators (TNF-alpha, IL-1beta, and IL-6) in the Lactoferrin immunization group compared to both the non-immunized and IFA immunized groups (Hwang et al., 2005). In concert with the increased stimulation index, there was significant production of all three proinflammatory mediators (TNF-alpha, IL-1beta, and IL-6) in the Lactoferrin immunization group compared to both the non-immunized and IFA immunized groups (Hwang et al., 2005). Lactoferrin was able to significantly augment the production of IFN-γ compared to vaccine preparations in IFA alone (Hwang et al., 2005). Significant down-regulation of both ferritin light- and heavy-chain (Thom et al., 2012). Dunkin Hartley (Thom et al., 2012). Pathogen-free, female outbred Dunkin Hartley strain guinea pigs (weighing 500–550 g) were vaccinated subcutaneously with 5 × 104 colony forming units (CFU) of M. bovis BCG Pasteur (Thom et al., 2012). PPD from M. tuberculosis was added to splenocytes from BCG-vaccinated guinea pigs at a final concentration of 30 μg/ml for 16 and 24 h at 37 °C in the presence of 5% CO2. The splenocyte cultures from naïve and BCG-vaccinated guinea pigs were infected with M. tuberculosis at an MOI of 0.2 and incubated for 4, 16 and 24 h at 37 °C in the presence of 5% CO2. The viability of uninfected and infected splenocytes from the same vaccination group was determined at 4, 16 and 24 h post-M. tuberculosis infection by trypan blue exclusion (Thom et al., 2012). Splenocytes from BCG-vaccinated guinea pigs were stimulated ex vivo with purified protein derivative from M. tuberculosis and a significant down-regulation of ferritin light- and heavy-chain was measured by reverse-transcription quantitative-PCR (Thom et al., 2012). Splenocytes from BCG-vaccinated guinea pigs were stimulated ex vivo with purified protein derivative from M. tuberculosis and a significant down-regulation of ferritin light- and heavy-chain was measured by reverse-transcription quantitative-PCR (Thom et al., 2012). A significantly greater number of total cells and CD8 T cells was observed in BCG vaccinated animals at day 60 post-challenge compared with unvaccinated animals. Immunohistochemistry revealed substantial higher number of CB8 cells in the lung lesions of vaccinated rats at day 30. Finally, a high number of mycobacteria-specific CD4 and CD8 T cells producing IFN-gamma was detected in BCG vaccinated rats (Singhal et al., 2011). Wistar rat Wistar rats were vaccinated with 10^6 BCG subcutaneously 6-8 weeks prior to challenge (Singhal et al., 2011). VO_0000286 A significantly reduced bacillary load was observed in the lungs of BCG vaccinated rats over the entire course of infection. About a 1 log(10) reduced bacillary load was observed in the lungs of vaccinated rats compared to unvaccinated animals (Singhal et al., 2011). The rats were challenged with a low dose of Mtb W4 strain 6 weeks after vaccination (Singhal et al., 2011). The study showed that BCG vaccinated Wistar rats efficiently control early bacillary growth and pathology related to Mtb infection (Singhal et al., 2011). The cattle that had been vaccinated with BCG showed significantly less numbers of lesions in the lungs and lymph nodes. Friesian Calves Vaccinated subcutaneously in the neck with 2 mL of 2X10^5 cfu BCG Cattle were examined 154 to 164 days after they had been challenged. VO_0000408 Vaccination with BCG induced considerable protection against a challenge with M. bovis, there were significantly fewer animals with Tuberculosis lesions. 54 days after vaccination the calves were challenged intratracheally with 2X10^3 cfu M. bovis strain 83/6235. This study showed that killed M. vaccae did not protect against challenge by M. bovis, however, the vaccination of cattle with BCG by the respiratory route protected them against the development of tuberculous lung lesions. In the vaccinated cattle group, increased IFN-gamma was released from the culture of peripheral blood lymphocytes from cattle after stimulation with bovine purified protein derivative (PPD) (Buddle et al., 1995). In the vaccinated cattle group, increased IL-2 was released from the culture of peripheral blood lymphocytes from cattle after stimulation with bovine purified protein derivative (PPD) (Buddle et al., 1995). Showed specific cell-mediated immune responses after bacillus Calmette–Guerin vaccination as observed by skin test, lymphoproliferation, and IFN-gamma production (Langermans et al., 2001). cynomolgus monkey (M. fascicularis) and rhesus monkey (M. mulatta) The macaques were vaccinated intradermally with 1–4 X 10^5 cfu bacillus Calmette–Guerin VO_0000859 17 weeks after vaccination the monkeys were challenged with 3,000 cfu M. tuberculosis by intratracheal installation of 3 ml of the bacterial suspension. Between 59 and 65 days after infection the animals were killed, and necropsies were undertaken for pathological examination (Langermans et al., 2001). This study showed that cynomolgus monkey (M. fascicularis) is a better model than rhesus monkey (M. mulatta) for M. tuberculosis vaccination and infection studies. From a challenge with 3,000 cfu M. tuberculosis, BCG vaccination protects cynomolgus monkeys with >2-log reduction of the bacterial load and diminished pathology, whereas only a minimal BCG vaccination effect was observed in rhesus monkeys. These two species represent the two extremes of BCG-induced protection that is found in humans (Langermans et al., 2001). Significantly smaller amounts of CFU of the bacterium were observed in the brains of rabbits that were vaccinated with BCG. No granulomas were found in the lungs of mice vaccinated with BCG while they were found in the unvaccinated group. Vaccination with BCG induced a statistically significant increase in antigen-specific T-cell proliferation compared to that of non-vaccinated controls (Tsenova et al., 2006). New Zealand White Rabbits Rabbits were vaccinated three times at 3-week intervals intramuscularly with a total of 500 micro liters of BCG (Tsenova et al., 2006). Rabbits were euthanized 8 weeks post-infection (Tsenova et al., 2006). VO_0000134 This study showed that vaccination with BCG induced protection against M. tuberculosis because rabbits that were vaccinated showed increased T-cell proliferation and no granulomas in the lungs (Tsenova et al., 2006). Ten weeks after immunization with BCG the rabbits were anesthetized and immobilized and a spinal needle was used to withdraw .3 mL of cerebrospinal fluid and .2 mL of 5X10^5 CFU of M. tuberculosis was injected intracisternally (Tsenova et al., 2006). Wildtype AB or adult rag hu1999 mutant fish Fish were vaccinated with ~8X10^3 CFU BCG intraperitoneally or intramuscularly (Oksanen et al., 2013). VO_0003057 BCG was unable to prevent infection with mycobacteria, however, it improved fish survival lowering the mortality rate from 84.2% to 45.0% (Oksanen et al., 2013). Fish were anesthetized and intraperitoneally injected 3-4 weeks after immunization with ~20-30 bacteria (Oksanen et al., 2013). BCG is unable to prevent infection in zebrafish, however, it is able to improve fish survival (Oksanen et al., 2013). BCG auxotroph expressing HIV-1 clade immunogen VO_0004122 Live, attenuated vaccine Goal of study was to develop a vaccine to minimize the risk of mother-to-child transmission (MTCT) of M. tuberculosis and HIV-1, and to protect against both pathogens in newborns. BCG was selected as a vaccine vector due to its long efficacy as a vaccine (10-15 years), frequent use in infant and newborn vaccination, and induction of humoral and cellular responses (Im et al., 2007). HIVA gene with mAb tag Pk was fused to M. tuberculosis 19-kDa lipoprotein signal sequence via PCR to improve foreign protein immunogenicity. HIVA was cloned into two plasmids, pJH222 and pJH223, as HindIII-HindIII fragment and used to transform a lysine auxotroph of BCG (BCG Pasteur lysA5::res). Additional preparations included MVA.HIVA and BCG.p. BCG cultures were grown in presence of kanamycin. HIVA immunogenicity was assessed using H-2D^d-restricted epitope P18-I10 and subdominant H-2K^d-restricted P epitope. MHC class II-restricted responses were tested using three epitope peptides derived from p24 (Im et al., 2007). HIVA, an African HIV-1-derived immunogen, was identified as stable and inductive of HIV-1-specific CD4+ and CD8+ T cell responses. HIVA in this study contained an immunodominant H-2D^d restricted epitope P18-I10 (Im et al., 2007). HIVA-specific responses peaked after 12 weeks, most likely as a result of BCG-induced activation of T cells. Three major observations were observed: 1) BCG.HIVA alone induces undetectable CD8+ HIV-1-specific T cell responses secreting tum, though priming with BCG.HIVA shows significant increases in elicited H and P-specific splenocytes producing IFN-gamma and degranulating. Hence, BCG.HIVA enhancement of MVA.HIVA responses is HIVA-specific. 2) BCG.HIVA priming with MVA.HIVA boost elicits the highest proportion of HIV-1 specific bifunctional cells in all the studies. 3) No HIVA-induced CD4+ T cell responses were detected (Im et al., 2007). BALB/c Mice were immunized on day 0 with either BCG.HIVA (pJH222.HIVA) and episomal plasmid, parental BCG (BCG.p), or control (no immunization). Half of the animals were boosted with MVA.HIVA on day 102. Mice were sacrificed on day 151. T cells were stimulated with peptides in a multicolor flow cytometric analysis to elicite interferon gamma, tumor necrosis factor alpha, and degranulating responses (Im et al., 2007). Bifunctional responses increased steadily with dose up to 0.7% CD8+ splenocytes with 10^6 CFU BCG.HIVA for epitope H , and increased up to 0.2% CD8+ with 10^7 CFU for P response (Im et al., 2007). Priming dose of BCG.HIVA affects strength and quality of CD8+ T cell responses induced by the recombinant BCG priming followed by recombinant MVA boosting, with high priming dose being most important for quality of response to P epitope (Im et al., 2007). BALB/c Mice were primed with increasing doses (10^3-10^7 CFU/animal, day 0) of BCG.HIVA or not vaccinated, and boosted (day 102) with constant doses of MVA.HIVA. BALB/c, 6-8 wk old Pathogen free mice were vaccinated in groups of 7-9 animals with 3x10^5 CFU of BCG.HIVA, BCG.p, or BCG wild type 1173 p2 via s.c. injection into left hind foot. All three vaccines tested here showed an approximate 2-fold reduction in M. tuberculosis load in the lungs and spleen. Data suggest that the "safer" lysine auxotrophBCG.HIVA could replace BCG vaccine in neonatal uses and still show comparable efficacy (Im et al., 2007). Mice were challenged via aerosol (nasal) route 12 weeks post-vaccination with M. tuberculosis Erdman using a modified Henderson apparatus. Approximately 25 CFU/lung were estimated 24 hours post-challenge (Im et al., 2007). No HIVA-specific responses were noticed during the 4-day WR.HIVA infection. Mice receiving the pTHr.HIVA-BCG.p, pTHr.HIVA-BCG.HIVA , or BCG.HIVA showed both H and P specific responses. Highest responses were seen in pTHr.HIVA-BCG.HIVA-WR.HIVA which showed interferon gamma and degranulated responses. CD4+IFN-gamma+TNF-alpha+IL-2+ splenocytes exhibited high quality characteristics in the BCG.HIVA and BCG.p boosts following pTHr.HIVA priming. HIVA non-specific mechanisms were suggested by a two-log10 decrease in WR.HIVA titre when BCG.HIVA or BCG.p alone were used in immunization (Im et al., 2007). BALB/c, female Groups of 4-5 mice were naive or immunized with 100 μg pTHr.HIVA, 10^6 pfu MVA.HIVA, or 10^6 cfu of either BCG.p or BCG.HIVA. BCG.HIVA boosting commenced on day 33. Mice were challenged i.p. using 4x10^6 pfu vaccinia virus WR.HIVA. Plaque counts were determined for sonicated and serially-diluted mouse ovaries four days post-challenge (Im et al., 2007). Surrogate challenge with recombinant replication-competent vaccinia virus (strain Western Reserve) is a cost-effective murine alternative to HIV-1challenge in non-human primate models. BCG expressing MSP VO_0004107 Live, attenuated vaccine Recombinant BCG (rBCG30) was prepared using E.coli/mycobacteria shuttle plasmid pSMT3. Plasmid was electoporated into the wild-type M. bovis BCG Tice strain. The 30-kDa MSP was isolated from the subcultured strains and further processed prior to inclusion in the vaccine (Horwitz et al., 2000). MSP, a 30-kDa major secretory protein or mycolyltransferase, is used as vaccine antigen (Horwitz et al., 2000). Hartley, 250-350g outbred male The highly susceptible guinea pig model of pulmonary TB, a model noteworthy for its close resemblance to human TB, was used for testing. Animals immunized with the recombinant BCG vaccine and challenged by aerosol with a highly virulent strain of M. tb. had 0.5 log fewer M. tb. bacilli in their lungs and 1 log fewer bacilli in their spleens on average than animals immunized with their parental conventional BCG vaccine counterparts. Statistically, the difference was highly significant. At necropsy, animals immunized with the recombinant BCG vaccine had fewer and smaller lesions in the lung, spleen, and liver and significantly less lung pathology than animals immunized with the parental BCG vaccine (Horwitz et al., 2000). Animals were challenged nine weeks after first injection or skin testing via aerosol with 10-ml single-cell suspension containing 1x10^5 CFU (1st & 2nd experiment) or 2x10^5 CFU (3rd experiment) of M. tuberculosis Erdman, providing ~40 to 80 liver bacilli to lungs, respectively (Horwitz et al., 2000). BCG mutant secreting listeriolysin VO_0000603 Subunit vaccine A recombinant BCG strain was constructed to secrete a pore-forming sulfhydrul-activated cytolysin (listeriolysin or Hly) from Listeria monocytogenes. Listeriolysin assists Listeria in escaping from the phagosome into the cytosol of the infected host cell. The study shows that Hly secretion improves stimulation of CD8 T cells (by pronounced MHC class I processing and delivery) in recombinant BCG (Hess et al., 1999). The r-BCG has decreased intracellular persistence versus the parental BCG strain (Hess et al., 1999). Mycobacterial CFUs from infected macrophages were used to ascertain survival of BCG pAT261:Hly and BCG pMV306:Hly in host macrophages. Intracelluar survival of the BCG pMV306:Hly strain was reduced at day 1 post-infection, and viable BCG pAT261:Hly were not detected in J774A.1 macrophages at 15 days post-infection. Furthermore, the recombinant strains failed to escape from the phagosome of macrophages (Hess et al., 1998). BCG pAT261:Hly and BCG pMV306:Hly with actively-secreted hemolysin were constructed using chromosomal integration of a mycobacterial shuttle vector (pAT261 or PMV306) or by episomal plasmid replication, respectively (Hess et al., 1998),(Hess et al., 1999). These plasmids were introduced into M. bovis BCG using standard electroporation. Fusion proteins were isolated and separated (Hess et al., 1998). In later studies, the fusion proteins were expressed by S. typhimurium strain SL7207's aroA pathway (Hess et al., 1999). The tuberculosis vaccine Mycobacterium bovis bacille Calmette-Guérin (BCG) was equipped with the membrane-perforating listeriolysin (Hly) of Listeria monocytogenes (Grode et al., 2005). BCG Vaccine BCG Live BCG Vaccine Organon Teknika Corp., LLC VO_0000771 Live, attenuated vaccine Licensed Percutaneous USA BCG VACCINE for percutaneous use, is an attenuated, live culture preparation of the Bacillus of Calmette and Guerin (BCG) strain of Mycobacterium bovis. The TICE® strain used in this BCG VACCINE preparation was developed at the University of Illinois from a strain originated at the Pasteur Institute. (FDA: BCG Vaccine) Stored at 2–8°C (36–46°F), protect from sunlight. Percutaneous BCG Vaccine (Freeze-Dried) BCG Vaccine (Freeze-Dried) Sanofi Pasteur Ltd VO_0010708 Live, attenuated vaccine Licensed Intradermal injection (i.d.) Canada Products: Live bacteria. Other components: Polysorbate 80. Intradermal injection (i.d.) BCG-DHTM VO_0004618 Recombinant vector vaccine Research subcutaneous injection subcutaneous injection BCG vaccine with deficient urease; a fusion protein composed of Mycobacterium tuberculosis-derived major membrane protein II (MMP-II) and heat shock protein 70 (HSP70) of BCG (Mukai et al., 2014). Recombinant vector construction The M. tuberculosis gene dnaK, also known as hsp70, is a part of a fusion gene composed of the M. tuberculosis-derived MMP-II gene and the hsp70 gene of M. tuberculosis (locus tag: Rv0350). This fusion gene is introduced into the urease-deficient BCG-deltaUT-11-3 strain (Mukai et al., 2014). Recombinant vector construction The M. tuberculosis gene bfrA (also known as MMP-II) is a part of a fusion gene composed of the M. tuberculosis-derived MMP-II gene and the hsp70 gene of M. tuberculosis (locus tag: Rv0350). This fusion gene is introduced into the urease-deficient BCG-deltaUT-11-3 strain (Mukai et al., 2014). C57BL/6J Five mice per group were vaccinated with BCG-261H (control) or BCG-DHTM at 1000 CFU/mouse for 6 weeks (Mukai et al., 2014) VO_0003057 At 6 weeks post challenge, mice vaccinated with BCG-261H (control) or BCG-DHTM showed inhibited multiplication of M. tuberculosis in the lung, and BCG-DHTM inhibited M. tuberculosis multiplication more strongly than BCG-261H (Mukai et al., 2014). Mice were challenged with H37RV at 100 CFU per lung by aerosol infection (Mukai et al., 2014) BG + MH/DPC Subunit vaccine Research subcutaneous injection BG + MH/DPC is a fusion protein subunit tuberculosis vaccine that combines BfrB–GrpE and Mtb10.4–HspX antigens in the DPC adjuvant system, resulting in enhanced immune responses and improved protection against Mycobacterium tuberculosis infection (Niu et al., 2023). The vaccine uses the DPC adjuvant system composed of DDA, poly(I:C), and cholesterol (Niu et al., 2023). subcutaneous injection The vaccine contains two antigens: the BfrB–GrpE fusion protein and the Mtb10.4–HspX fusion protein from Mycobacterium tuberculosis (Niu et al., 2023). Fusion Protein Use in fusion protein preparation(Niu et al., 2023). Fusion Protein This is use in fusion protein preparation (Niu et al., 2023) . Fusion Protein This is use in preparation of fusion protein (Niu et al., 2023). Fusion Protein This is use in fusion protein preparation (Niu et al., 2023). C57BL/6 Mice were immunized subcutaneously at weeks 0, 4, and 12 with BG + MH/DPC, receiving 200 µL per dose containing equal amounts of BfrB–GrpE and Mtb10.4–HspX fusion proteins formulated with the DPC adjuvant(Niu et al., 2023). BG + MH/DPC significantly reduced bacterial burden in the lungs and spleens and provided protective efficacy comparable to BCG vaccination (Niu et al., 2023). Twelve weeks after the final immunization, mice were challenged intravenously with Mycobacterium tuberculosis H37Ra or by aerosol exposure to virulent H37Rv, and bacterial loads in the lungs and spleens were quantified (Niu et al., 2023). BG-Lentinan Subunit vaccine Research subcutaneous injection BG–Lentinan is a fusion protein subunit vaccine composed of the BfrB–GrpE fusion protein formulated with lentinan as an adjuvant, which induces strong antigen-specific IgG responses and provides effective protection by significantly reducing bacterial burden in infected tissues (Zhou et al., 2025). Adjuvant is Lentinan (LNT) (Zhou et al., 2025). BG fusion protein expressed in E. coli BL21, purified by ammonium sulfate precipitation and hydrophobic interaction chromatography. Mixed with lentinan and Phosphate-Buffered Saline (Zhou et al., 2025). subcutaneous injection Fusion protein BG, composed by BfrB and GrpE from Mycobacterium tuberculosis (Zhou et al., 2025). Fusion Protein This is use in fusion protein preparation (Zhou et al., 2025). Fusion Protein Use in prepare fusion protein (Zhou et al., 2025). BG–Lentinan vaccination significantly increased BfrB- and GrpE-specific IgG levels in rabbit serum measured six weeks after the final immunization (Zhou et al., 2025). Rabbits were immunized subcutaneously with BG–Lentinan at weeks 0, 2, and 4, receiving 1000 µL per dose containing 100 µg of BG fusion protein and 500 µg of lentinan (Zhou et al., 2025). The BG–Lentinan vaccine significantly reduced bacterial load in skin lesions and showed the strongest protective efficacy among all tested groups (Zhou et al., 2025). Six weeks after the final immunization, rabbits were challenged intradermally in the dorsal skin with 5 × 10⁶ CFU of Mycobacterium bovis BCG in a 100 µL volume (Zhou et al., 2025). BG–Lentinan/Mn(J) Subunit vaccine Research subcutaneous injection BG–Lentinan/Mn(J) is a fusion protein subunit vaccine combining the BfrB–GrpE fusion protein with lentinan and a manganese-based adjuvant, which enhances antibody responses and accelerates lesion healing, but does not surpass BG–Lentinan alone in bacterial clearance (Zhou et al., 2025). Adjuvant is combined by Lentinan (LNT) and Mn(J) (Zhou et al., 2025). The BG fusion protein was recombinantly expressed in E. coli, purified by ammonium sulfate precipitation and hydrophobic interaction chromatography, and formulated with lentinan alone and lentinan plus Mn(J) in PBS for subcutaneous immunization (Zhou et al., 2025). subcutaneous injection Fusion protein BG (BfrB–GrpE) derived from Mycobacterium tuberculosis (Zhou et al., 2025). Fusion Protein This is use in fusion protein preparation (Zhou et al., 2025). Fusion Protein This is use in fusion protein preparation (Zhou et al., 2025). Rabbits received subcutaneous immunization with BG–Lentinan/Mn(J) at weeks 0, 2, and 4, with each 1000 µL dose containing 100 µg of BG fusion protein, 500 µg of lentinan, and 500 µg of Mn(J)(Zhou et al., 2025). The BG–Lentinan/Mn(J) vaccine significantly reduced bacterial burden and accelerated lesion healing, showing protective efficacy comparable to BCG vaccination (Zhou et al., 2025). Rabbits were challenged six weeks after the final immunization by intradermal injection of 5 × 10⁶ CFU of Mycobacterium bovis BCG into the dorsal skin (Zhou et al., 2025). BG–Lentinan/Mn(J) is a tuberculosis protein subunit vaccine that combines the BfrB–GrpE fusion protein with lentinan and a manganese-based adjuvant to enhance immunogenicity and protective efficacy in rabbits (Zhou et al., 2025). CTB-ESAT6-MPT64 Subunit vaccine Research Mucosal (oral) immune route The adjuvant is cholera toxin B subunit (CTB)(Yadav et al., 2023). Mucosal (oral) immune route The antigen is composed of Mycobacterium tuberculosis antigens ESAT-6 and MPT-64 (Yadav et al., 2023). Fusion Protein This is use in fusion protein preparation (Yadav et al., 2023). Fusion Protein It's use in prepare fusion protein (Yadav et al., 2023). Oral administration of transgenic cucumber expressing CTB-ESAT-6-MPT-64 induced a significant increase in antigen-specific serum IgG levels compared with rabbits fed non-transgenic cucumber, demonstrating effective immunogenicity of the fusion protein (Yadav et al., 2023). Rabbits were orally immunized by feeding 3 g of transgenic cucumber fruit expressing the CTB-ESAT-6-MPT-64 fusion protein on days 0, 7, and 14, with serum collected on day 21 for immunological analysis (Yadav et al., 2023). DNA vaccine co-expressing A85A and caspase-3 VO_0004148 DNA vaccine pBudCE4.1 [Ref698:Gartner et al., 2008] Native Ag85A was purified from BCG culure and recombinant his-tagged Ag85A was purified by affinity chromatography. Dual expression-vectors were created encoding mature or secreted Ag85A and either mutant or wild-type caspase-3 (Gartner et al., 2008). The antigens for this vaccine were wild-type or mutated caspase-3 inserted into a plasmid that encodes mycolyl transferase antigen 85A (Ag85A) (Gartner et al., 2008). DNA vaccine construction Ag85A specific antibody titers were very low in mice vaccinated with the wild-type p-sAg85A–csp, but antibody titers in mice vaccinated with mutant p-sAg85A–csp were of a greater magnitude. IFN-γ and IL-2 production in mice vaccinated with plasmids co-expressing mAg85A and a caspase gene were similar to or lower than production in mice vaccinated with p-sAg85A. Vaccination with the wild-type caspase-encoding plasmids was not effective in inducing Ag85A specific CTL activity (Gartner et al., 2008). (C57BL/6 × DBA/2)F1 (H-2b/d) or BALB/c (H-2d) Mice were injected four times intramuscularly with 100 micrograms of pDNA with at least three weeks between injections. As negative controls, mice were injected with 100 micrograms of empty pBudCE4.1 vector or left untreated. As a positive control for the challenge experiments, mice were vaccinated intravenously with M. bovis BCG strain GL2 along with the first vaccination or 5 weeks pre-vaccination (Gartner et al., 2008). The challenge revealed that mice vaccinated with mutant p-sAg85A–csp showed higher secretion of IL-6, IL-10 and IL-17A in their lungs, spontaneously and/or after antigenic stimulation.However, spontaneous IFN-γ production was highest in mice vaccinated with wild-type p-sAg85A–csp. Ag85A specific IL-2 and IFN-γ responses were low in spleen of BCG vaccinated mice, but high Th1 responses could be could be seen after stimulation with whole BCG culture filtrate in these mice (Gartner et al., 2008). Mice were challenged five weeks after their final vaccination with 2 x 10^5 CFUof M. tuberculosis H37Rv. Results were measured using a luminescence assay with a count of relative light units (RLU) (Gartner et al., 2008). DNA vaccine Rv1806-1807 VO_0000567 DNA vaccine pET11d, modified to form pET3a [Ref329:Vipond et al., 2006] Rv1806-1807 was one of three potential vaccines compared against gold-standard BCG vaccine and saline control. Rv1806-1807 induced protection in the guinea pig aerosol infection model 30 days post-challenge on the basis of reducing the bacterial burden of M tuberculosis in the lungs ( a reduction in log10 cfu of 0 . 52 and 0 . 83 in lungs and spleen , respectively , following DNA vaccination , compared with reductions of 0 . 3 and 0 . 57 , respectively , with a protein formulation) (Vipond et al., 2006). Dioctadecylammonium (DDA), trehalose dibehenate (TDB), and stable liposomes (SSI) (Vipond et al., 2006). Not virulent. Two bacterial strains, E. coli DH5α and Rosettaâ„¢ 2(DE3)pLysS, were propagated in Luria Bertani (LB) broth and solid medium at 37°C. An expression vector pET3a was created from pET11d expression vector (Novagen) by replacing an ampicillin cassette with kanamycin cassette, followed by restriction digestion of a HindIII site and linker insertion containing cloning sites and an N- and C-terminal 6* histidine tag. The vaccine candidate gene was amplified from M. tuberculosis using PCR and cloned into pVAX1tPA. Recombinant plasmids were generated from four encoding gene sequences and sub-cloned into pET3a prior to transformation, induction, and harvesting in E. coli Rosettaâ„¢ 2(DE3)pLysS (Vipond et al., 2006). Rv1806 and Rv1807 (Vipond et al., 2006). DNA vaccine construction DNA vaccine construction Dunkin-Hartley guinea pig Female Dunkin-Hartley guinea pigs were vaccinated by injecting 100 μg of protein intramuscularly into both hind quadriceps muscles per guinea pig in 100 μl of dioctadecylammonium (DDA) and trehalose dibehenate (TDB) (500 μg DDA plus 100 μg TDB) stable liposomes (SSI). Sub-cutaneous inoculation with 5×10^4 CFU BCG Pasteur was used as positive control. Saline was used as negative control. Ten guinea pigs were used for each group. Vaccinated animals were booster vaccinated with 200 μg protein in adjuvant after 3 and 6 weeks (Vipond et al., 2006). Level of protection by Rv1806-1807 at week 15 post challenge was significantly better than saline (p=.018) and comparable to related DNA vaccinations. However, the protection was significantly less than gold-standard BCG. An antigen dose of 10 μg/ml of Rv1806-1807 vaccine stimulated the better protection than for 1 μg/ml (Vipond et al., 2006). Six weeks after the final vaccination, six animals per group were aerosol challenged with approximately 50 CFU of M. tuberculosis H37Rv (NCTC 7416) each in a contained Henderson apparatus. Protection was assessed by CFU in the lungs and spleen at 30 days post-challenge (Vipond et al., 2006). Dodecin–ESAT-6 Subunit vaccine Research subcutaneous injection DPL adjuvant contain DDA (dimethyl dioctadecyl ammonium bromide) and MPL (monophosphoryl lipid A) (Zhao et al., 2021). The dodecin–ESAT-6 fusion protein was generated by PCR amplification and SOEing fusion of the Rv1498A and Rv3875 genes, cloned into the pET-28a expression vector, expressed in Escherichia coli Rosetta (DE3), purified by His-tag affinity chromatography, and subjected to endotoxin removal prior to immunization (Zhao et al., 2021). subcutaneous injection The antigen is dodecin–ESAT-6 fusion protein, composed ESAT-6 (Rv3875) and fused to the dodecameric flavin-binding protein dodecin (Rv1498A) (Zhao et al., 2021). Fusion Protein Used in preparation of fusion protein (Zhao et al., 2021). Fusion Protein Used in preparation of fusion protein Immunization with dodecin–ESAT-6 elicited enhanced antigen-specific CD4⁺ and CD8⁺ T cell responses, increased IFN-γ and IL-2 secretion, reduced IL-4 production, and elevated IgG and IgG2a antibody titers, indicating a predominantly Th1-type immune response(Zhao et al., 2021). BALB/c Mice were immunized subcutaneously at weeks 0, 3, and 6 with 20 µg of dodecin–ESAT-6 formulated with the DDA/MPL (DPL) adjuvant, and immune responses were assessed four weeks after the final dose (Zhao et al., 2021). Dodecin–ESAT-6 vaccine enhance antigen presentation and induce strong Th1-skewed immune responses (Zhao et al., 2021). DR2/DIMQ Subunit vaccine Research subcutaneous injection The adjuvant is DIMQ, a liposomal formulation containing DDA and imiquimod (Mao et al., 2023). The DR2 fusion protein is produced by combining Rv0572c and Rv3621c and is formulated with the DIMQ liposomal adjuvant (Mao et al., 2023). subcutaneous injection The antigen is a fusion protein (DR2) composed of Rv0572c and Rv3621c from Mycobacterium tuberculosis (Mao et al., 2023). Fusion Protein This is use in fusion protein preparation (Mao et al., 2023). Fusion Protein This is use in fusion protein preparation (Mao et al., 2023). C57BL/6 Mice were first primed with BCG, followed by boost immunizations with the DR2/DIMQ vaccine at specific intervals. After vaccination, immune responses were assessed through cytokine production and memory T-cell analysis, and protection was evaluated against Mycobacterium tuberculosis(Mao et al., 2023). The vaccine demonstrated protective effects by inhibiting the growth of M. tuberculosis and enhancing immune protection compared to BCG alone (Mao et al., 2023). Protective efficacy was evaluated using in vitro infection models with Mycobacterium tuberculosis (Mao et al., 2023). ERA005f Subunit vaccine Research subcutaneous injection ERA005f is a multistage recombinant fusion protein tuberculosis vaccine formulated with alum that induces strong Th1 and Th17 immune responses and inhibits Mycobacterium tuberculosis growth in a mouse model (Fan et al., 2023). The vaccine was formulated with alum (Fan et al., 2023). The ERA005f fusion protein was expressed in E. coli, purified using nickel affinity and ion-exchange chromatography, refolded, and then mixed with alum adjuvant (Fan et al., 2023). subcutaneous injection ERA005f is a fusion protein composed of ESAT-6 (Rv3875), Rv2628, and Ag85B (Rv1886c) M. tuberculosis antigens (Fan et al., 2023). Fusion Protein This is use in fusion protein preparation (Fan et al., 2023). Fusion Protein This is use in fusion protein preparation (Fan et al., 2023). Fusion Protein This is use in fusion protein preparation (Fan et al., 2023). BALB/c Mice were immunized three times at 0, 2, and 4 weeks, and samples were collected 10 days after the final immunization (Fan et al., 2023). ERA005f significantly reduced H37Rv bacterial growth in mouse splenocytes (approximately 0.88 log10 CFU reduction compared to PBS control) (Fan et al., 2023). Protection was evaluated using a Mycobacterial Growth Inhibition Assay (MGIA) rather than in vivo aerosol challenge (Fan et al., 2023). GamTBvac Subunit vaccine Clinical trial subcutaneous injection GamTBvac is a recombinant fusion protein tuberculosis vaccine containing Ag85A, ESAT-6, and CFP10, formulated with CpG and a dextran-based adjuvant system, and designed as a BCG booster (Tkachuk et al., 2020). Adjuvant containing dextran 500 kDa, diethylaminoethyl (DEAE)-dextran 500 kDa, and CpG oligodeoxynucleotides (ODN) (Tkachuk et al., 2020). GamTBvac is made of two combined (fusion) tuberculosis proteins mixed with a dextran-based adjuvant and CpG to help stimulate the immune system, and it is prepared as a liquid injection given under the skin (Verreck et al., 2001). subcutaneous injection GamTBvac contains ESAT6, CFP10, and Ag85A Mycobacterium tuberculosis (Tkachuk et al., 2020) Fusion protein This is us in fusion protein preparation (Tkachuk et al., 2020). Recombinant protein preparation Use in fusion protein prepsrsption (Tkachuk et al., 2020). Fusion Protein This is use in fusion protein preparation(Tkachuk et al., 2020). GamTBvac induced significant IFN-γ release, increased Th1 cytokine-expressing CD4⁺ T cells, and strong antigen-specific IgG antibody responses (Tkachuk et al., 2020). Participants received two subcutaneous injections of GamTBvac (0.5 mL each) administered eight weeks apart (Day 0 and Day 57) (Tkachuk et al., 2020). H1 / CAF01 Subunit vaccine Research subcutaneous injection The adjuvant is CAF01. Composed of cationic liposomes (DDA) and trehalose-6,6-dibehenate (TDB) (Klessing et al., 2020). The H1 tuberculosis subunit vaccine was prepared by formulating a recombinant fusion protein consisting of Ag85B and ESAT-6 with the liposomal adjuvant CAF01. Prior to immunization, the H1 antigen was allowed to adsorb to the CAF01 adjuvant for 60 minutes on ice before subcutaneous injection (Klessing et al., 2020). subcutaneous injection H1 fusion protein composed of Ag85B and ESAT-6 from Mycobacterium tuberculosis (Klessing et al., 2020). Fusion Protein This is use in fusion protein preparation (Klessing et al., 2020). Fusion Protein This is use in fusion protein preparation (Klessing et al., 2020). C57BL/6 mice Mice were immunized twice at a three- to four-week interval with the H1 fusion protein formulated in CAF01, followed by additional booster immunizations depending on the experimental design (Klessing et al., 2020). The H1/CAF01 vaccine is a subunit fusion protein vaccine composed of a recombinant Ag85B–ESAT-6 fusion protein formulated with the cationic liposomal adjuvant CAF01. In mice, this vaccine induces strong, long-lasting Th1- and Th17-biased immune responses and supports durable antibody and plasma cell formation (Klessing et al., 2020). H107 Subunit vaccine Research subcutaneous injection The vaccine is formulated with CAF®01, a cationic liposome adjuvant consisting of DDA and TDB (Woodworth et al., 2021). The H107 antigen was produced as a recombinant multi-antigen fusion protein and formulated with the CAF®01 adjuvant, a cationic liposome system composed of DDA and TDB, prior to subcutaneous administration (Woodworth et al., 2021). subcutaneous injection H107 is a recombinant fusion protein composed of eight Mycobacterium tuberculosis antigens, including PPE68, ESAT-6 (four repeats), EspI, EspC, EspA, MPT64, MPT70, and MPT83 (Woodworth et al., 2021). Fusion Protein This is use for fusion protein preparation (Woodworth et al., 2021). Fusion Protein This is use in fusion protein preparation (Woodworth et al., 2021). Fusion Protein This is use in fusion protein preparation (Woodworth et al., 2021). Fusion Protein This is use in fusion protein preparation (Woodworth et al., 2021). Fusion Protein Use in fusion protein preparation (Woodworth et al., 2021). Fusion Protein This is use in fusion protein preparation (Woodworth et al., 2021). Fusion Protein This is use in fusion protein preparation (Woodworth et al., 2021). Fusion Protein Use in fusion protein preparation (Woodworth et al., 2021). CB6F1 and B6C3F1 Mice were immunized three times subcutaneously with H107 formulated in CAF®01 at two-week intervals, either alone or co-administered with a single BCG vaccination (Woodworth et al., 2021). H107 alone provided protection superior to BCG, and co-administration of BCG with H107 resulted in synergistic and sustained reduction in lung bacterial burden (Woodworth et al., 2021). Vaccinated mice were challenged with aerosolized Mycobacterium tuberculosis Erdman strain, and lung bacterial burden was measured at 4 and 18 weeks post-infection (Woodworth et al., 2021). H4-IC31 Subunit vaccine Clinical trial Intramuscular injection (i.m.) IC31 vaccine adjuvant consists of the antibacterial peptide KLK and oligodeoxynucleotide ODN1a (TLR9 agonist) (Rozot et al., 2020). The vaccine is prepared by formulating the recombinant H4 fusion protein (Ag85B–TB10.4) with the IC31 adjuvant. Each dose contained 15 μg of H4 protein combined with 500 nmol of IC31 and was administered under controlled clinical trial conditions (Rozot et al., 2020). Intramuscular injection (i.m.) H4 fusion protein composed of Ag85B and TB10.4 from Mycobacterium tuberculosis (Rozot et al., 2020). Fusion Protein This is use in fusion protein preparation (Ming et al., 2019). Fusion Protein This is use in fusion protein preparation (Rozot et al., 2020). Participants received intramuscular injections of the H4:IC31 vaccine. The vaccination schedule consisted of two doses administered on day 0 and day 56. Each dose contained 15 μg of H4 fusion protein formulated with 500 nmol of IC31 adjuvant (Rozot et al., 2020). H4:IC31 induced strong Th1-biased cellular immune responses and showed a trend toward protection against sustained M. tuberculosis infection; however, protective efficacy did not reach statistical significance (Rozot et al., 2020). H56:IC31 Subunit vaccine Clinical trial Intramuscular injection (i.m.) The adjuvant is IC31 (Tait et al., 2024). Intramuscular injection (i.m.) H56 is a recombinant fusion protein composed of Ag85B, ESAT-6 and Rv2660c (Tait et al., 2024). Fusion Protein This is use in fusion protein preparation (Tait et al., 2024). Fusion Protein This is use in fusion protein preparation (Tait et al., 2024). Fusion Protein This is use in fusion protein preparation (Tait et al., 2024). The vaccine induced strong antigen-specific CD4⁺ T-cell responses characterized by IFN-γ production, with ESAT-6 being immunodominant, and generated measurable H56-specific IgG antibody responses (Tait et al., 2024). Adults recently treated for drug-susceptible pulmonary tuberculosis received two intramuscular injections of 5 µg H56 formulated with 500 nmol IC31, administered on Day 0 and Day 56, with follow-up visits conducted to assess safety and immunogenicity up to several months after the second dose (Tait et al., 2024). H56:IC31 was generally well tolerated, with mostly mild to moderate local reactions such as injection site pain, swelling, and redness, and no serious vaccine-related adverse events were reported (Tait et al., 2024). H65 + CAF01 Subunit vaccine Research subcutaneous injection Adjuvant is CAF01, contain trehalose dibehenate (TDB) and dimethyldioctadecylammonium (DDA)(Bertholet et al., 2010). subcutaneous injection H65 fusion protein that composed of EsxD, EsxC, EsxG, EsxH, EsxW, EsxV (Bertholet et al., 2010). Fusion Protein This is use in fusion protein preparation(Blanco et al., 2021). Fusion Protein Use in fusion protein preparation(Blanco et al., 2021). Fusion Protein This is use in fusion protein preparation(Blanco et al., 2021). Fusion Protein Use in fusion protein preparation(Blanco et al., 2021). Fusion Protein Use in fusion protein preparation(Blanco et al., 2021). Fusion Protein This is use in fusion protein preparation(Blanco et al., 2021). Vaccination with H65 + CAF01, particularly in combination with BCG, induced enhanced protective immune responses resulting in significantly reduced Mycobacterium bovis bacterial burden in the lungs following challenge(Blanco et al., 2021). BALB/c Mice were vaccinated subcutaneously with H65 formulated in CAF01, BCG, or a combination of BCG and H65. H65 and BCG+H65 groups received two booster doses of H65 formulated in CAF01 at 2-week intervals following primary vaccination(Blanco et al., 2021). The BCG + H65 prime-boost regimen significantly reduced lung bacterial loads compared with BCG or H65 alone, demonstrating improved protective efficacy against Mycobacterium bovis infection in mice(Blanco et al., 2021). Vaccinated mice were challenged by aerosol exposure with approximately 2000 CFU of virulent Mycobacterium bovis using an intrapulmonary aerosol chamber. Lung bacterial burden was assessed four weeks post-challenge by CFU enumeration(Blanco et al., 2021). HSP90-ESAT-6-HspX-RipA Subunit vaccine Research subcutaneous injection The vaccine was formulated with CIA09A, a cationic liposome-based adjuvant system containing DOTAP/DMPC liposomes, the TLR4 agonist de-O-acylated lipooligosaccharide (dLOS), and QS-21(Kwon et al., 2024). The HEHR fusion gene was constructed by sequential PCR and overlap-extension cloning of HSP90-ESAT-6 with HspX-RipA, inserted into the pET22b vector, expressed recombinantly, purified, and formulated with lyophilized CIA09A prior to immunization; a tag-free version of HEHR was also produced for validation(Kwon et al., 2024). subcutaneous injection The antigen is composed of HSP90, ESAT-6, HspX and RipA Mycobacterium tuberculosis antigens(Kwon et al., 2024). Fusion Protein Use in fusion protein preparation(Kwon et al., 2024). Fusion Protein Use in preparing fusion protein(Kwon et al., 2024). Fusion Protein Use in fusion protein preparation(Kwon et al., 2024). Fusion Protein Use in fusion protein preparation(Kwon et al., 2024). BCG-primed HEHR/CIA09A boosting induced robust antigen-specific multifunctional CD4⁺ T-cells producing IFN-γ, IL-17A, TNF-α, and IL-2, with increased CXCR3⁺KLRG1⁻ effector phenotypes in the lung, indicating durable Th1/Th17 immunity(Kwon et al., 2024). C57BL/6 Mice were primed subcutaneously with BCG, followed by three booster immunizations with HEHR/CIA09A administered subcutaneously or intramuscularly at 3-week intervals, and subsequently challenged with M. tuberculosis(Kwon et al., 2024). BCG-primed HEHR/CIA09A boosting significantly reduced lung and spleen bacterial burden and pulmonary inflammation, providing superior and long-lasting protection compared to BCG alone or earlier subunit vaccines(Kwon et al., 2024). Four weeks after the final booster, mice were aerosol-challenged with hypervirulent Mycobacterium tuberculosis Haarlem strain M2 (~200 CFU) and analyzed 12 weeks post-infection(Kwon et al., 2024). HspX–PPE44–EsxV Subunit vaccine Research Intramuscular injection (i.m.) This is a recombinant fusion protein tuberculosis vaccine combining HspX, PPE44, and EsxV antigens with a liposomal adjuvant (Lipo-AE) to enhance T-cell–mediated immune responses and improve protection against Mycobacterium tuberculosis (Mansury et al., 2019). Lipo-AE (liposome-based adjuvant formulation) adjuvant is used (Mansury et al., 2019). The recombinant fusion protein (HspX–PPE44–EsxV) is produced and then formulated with the Lipo-AE liposomal adjuvant to enhance immune response (Mansury et al., 2019). Intramuscular injection (i.m.) Fusion protein composed by HspX, PPE44 and EsxV (Mansury et al., 2019). Fusion Protein This is use in fusion protein preparation (Mansury et al., 2019). Fusion Protein This is use in fusion protein preparation (Mansury et al., 2019). Fusion Protein This is use in fusion protein preparation (Mansury et al., 2019). C57BL/6 Mice were immunized with 50 μg antigen on days 0, 14, and 28, with some groups receiving BCG prime at day 0 followed by booster doses of fusion protein or liposome-formulated fusion protein on days 14 and 28 (Mansury et al., 2019). The vaccinated groups showed significantly reduced bacterial load in lungs and spleen compared to control groups. This indicates enhanced protective immunity against M. tuberculosis infection.(Mansury et al., 2019). ID93 + GLA-SE Subunit vaccine Clinical trial Intramuscular injection (i.m.) Adjuvant GLA-SE is use in this vaccine (Day et al., 2021). The recombinant ID93 fusion protein is expressed and purified, then mixed with the GLA-SE adjuvant (oil-in-water emulsion containing a TLR4 agonist) to create the final injectable vaccine formulation (Day et al., 2021). Intramuscular injection (i.m.) The antigen is ID93, which is a fusion protein made of 4 Mycobacterium tuberculosis antigens (Rv2608, Rv3619, Rv3620, Rv1813) (Day et al., 2021). Fusion Protein This is use in fusion protein preparation (Day et al., 2021). ID93 was generated through a tandem fusion of the individual cloned and amplified genes of Rv3619, Rv1813, Rv3620, and Rv2608 using restriction site linkers.(Bertholet et al., 2010) Fusion Protein This is use in fusion protein preparation (Day et al., 2021). ID93 was generated through a tandem fusion of the individual cloned and amplified genes of Rv3619, Rv1813, Rv3620, and Rv2608 using restriction site linkers.(Bertholet et al., 2010) Fusion Protein This is use in fusion protein preparation (Day et al., 2021). ID93 was generated through a tandem fusion of the individual cloned and amplified genes of Rv3619, Rv1813, Rv3620, and Rv2608 using restriction site linkers.(Bertholet et al., 2010) Fusion Protein This is use for fusion protein preparation (Day et al., 2021). ID93 was generated through a tandem fusion of the individual cloned and amplified genes of Rv3619, Rv1813, Rv3620, and Rv2608 using restriction site linkers.(Bertholet et al., 2010) Vaccination generated strong antigen-specific IgG antibody responses and polyfunctional CD4⁺ T-cell responses that were durable and significantly higher than placebo (Day et al., 2021). Participants received intramuscular injections of ID93 + GLA-SE on day 0 and day 56, with some groups receiving an additional dose on day 28 (prime–boost or 3-dose schedule depending on cohort) (Day et al., 2021). ID93/GLA-SE Subunit vaccine Clinical trial Intramuscular injection (i.m.) Intramuscular injection (i.m.) Recombinant protein preparation D93 was generated through a tandem fusion of the individual cloned and amplified genes of Rv3619, Rv1813, Rv3620, and Rv2608 using restriction site linkers.(Bertholet et al., 2010) Recombinant protein preparation ID93 was generated through a tandem fusion of the individual cloned and amplified genes of Rv3619, Rv1813, Rv3620, and Rv2608 using restriction site linkers.(Bertholet et al., 2010) Recombinant protein preparation ID93 was generated through a tandem fusion of the individual cloned and amplified genes of Rv3619, Rv1813, Rv3620, and Rv2608 using restriction site linkers.(Bertholet et al., 2010) Recombinant protein preparation ID93 was generated through a tandem fusion of the individual cloned and amplified genes of Rv3619, Rv1813, Rv3620, and Rv2608 using restriction site linkers.(Bertholet et al., 2010) ΔureC hly+ rBCG VO_0000606 Live, attenuated vaccine Research Intramuscular injection (i.m.) In order to generate a live vaccine that provides improved protection against M. tuberculosis, BCG was equipped with the membrane-perforating Hly of L. monocytogenes (hly+ rBCG). To improve the pH milieu for Hly activity, a ΔureC hly+ rBCG strain that lacked ureC activity was constructed (Grode et al., 2005). Intramuscular injection (i.m.) Mice were immunized i.v. with 10^6 CFU of BCG strains (Grode et al., 2005). The ΔureC hly+ rBCG vaccine strain induced significantly more efficacious in inducing protective immunity against 2 different M. tuberculosis strains, the laboratory strain H37Rv and a clinical isolate of the Beijing/W genotype family, in a mouse model, than parental BCG (Grode et al., 2005). The ΔureC hly+ rBCG vaccine provided even more efficient protection than the hly+ rBCG vaccine strain. At 120 days after vaccination, animals were aerosol challenged with 100–200 CFU of M. tuberculosis H37Rv or Beijing/W per lung using an aerosol chamber (Glas-Col) (Grode et al., 2005). licensed Tuberculosis human vaccine Generic Unknown VO_0000077 Live, attenuated vaccine Licensed A generic representation of vaccines used to prevent tuberculosis in humans, most notably the Bacillus Calmette-Guérin (BCG) vaccine, which is a live, attenuated strain of Mycobacterium bovis. These vaccines stimulate immune protection against Mycobacterium tuberculosis infection. LT-BCG- Ag85B /Rv3425 VO_0004626 Recombinant vector vaccine Research [Ref3074:Xu et al., 2014] Intramuscular injection (i.m.) DNA-, protein- and lentiviral vector-based vaccines that express the antigens Ag85B and Rv3425 (Xu et al., 2014). Intramuscular injection (i.m.) Recombinant vector construction DNA-, protein- and lentiviral vector-based vaccines that express the antigens Ag85B and Rv3425 (Xu et al., 2014). C57BL/6 Six mice per group were first immunized subcutaneously (s.c.) with 5×10^6 CFU of BCG in 100μl of PBS and subsequently boosted twice at 2-week intervals with PAR, DAR or LAR [12]. Meanwhile, the mice were boosted with the controls for these three types of vaccines, which were PNC (i.e., MPL + TDM), DNC (i.e., pVax) and LNC (i.e.,pLenti6.3). For DAR immunization, 50μg of the DAR DNA vaccine in 100μl of sterile PBS was administered to the mice intramuscularly (i.m.) in the right thigh. For LAR immunization, the mice were immunized with 5×10^6 pfu of LAR in the foot pad. For PAR immunization, the mice were immunized subcutaneously (s.c.) with 50 μg of PAR formulated with the adjuvants MPL and TDM (Xu et al., 2014). VO_0003057 Prime-boost BCG vaccination with a lentiviral vector expressing the antigens Ag85B and Rv3425 significantly enhanced immune responses, including Th1 and CD8+ CTL responses, compared to DNA- and protein-based vaccines. However, lentivirus-vectored and DNA-based vaccines greatly improved the protective efficacy of BCG against M. tuberculosis (Xu et al., 2014). 2 weeks after the last vaccination, all mice were challenged intravenously via the lateral tail vein with 1.2 × 10^6 CFU of M. tuberculosis H37Rv (Xu et al., 2014). LV20/DP Subunit vaccine Research The antigen is displayed on influenza virus-like particles (VLPs) formed by influenza hemagglutinin (HA) and matrix protein M1, which serve as a non-replicating antigen presentation platform.[Ref6778:Wang et al., 2023] subcutaneous injection Adjuvant is DP that consist of dimethyl dioctadecylammonium (DDA) and Poly I:C. (Wang et al., 2023) The purified virus-like particles were stored at −80 °C prior to use.(Wang et al., 2023) The vaccine was prepared using a baculovirus–insect cell (Sf9) expression system and purify virus-like particles by using sucrose gradient ultracentrifugation (Wang et al., 2023). subcutaneous injection The vaccine antigen is a fusion protein (L20) composed of HBHA and MTP from Mycobacterium tuberculosis, which are displayed on virus-like particles (Wang et al., 2023). Fusion Protein This is use for fusion protein preparation(Wang et al., 2023). Fusion Protein Use in preparation of fusion protein(Wang et al., 2023). Vaccination with LV20 formulated with DDA and Poly I:C elicited strong HBHA-specific CD4⁺ and CD8⁺ T cell responses, with increased production of IFN-γ and IL-2, as well as elevated levels of HBHA-specific IgG, IgG1, and IgG2c antibodies(Wang et al., 2023). C57BL/6 Mice were immunized subcutaneously with LV20, HBHA/DP, or LV20/DP on days 0, 28, and 84, while BCG and PBS were administered as single-dose controls (Wang et al., 2023). Vaccine-induced immune responses persisted for several weeks after the final immunization, as antigen-specific T cell responses and antibody levels were detected up to 42–84 days post-immunization (Wang et al., 2023). Vaccination with LV20 formulated with DDA and Poly I:C significantly reduced lung bacterial burden by approximately 1.2–1.3 log₁₀ CFU compared to control groups (Wang et al., 2023). Immunized mice were challenged intranasally with 5 × 10⁶ CFU of Mycobacterium tuberculosis H37Ra, and lung bacterial loads were measured 21 days post-challenge(Wang et al., 2023). M. bovis WAg520 strain VO_0000552 Attenuated vaccine WAg520, an attenuated strain of M. bovis, has only a 2-bp deletion in a possible undecaprenol kinase gene. It is one of two nutritionally impaired strains which produced significant protection in the lungs. The other one is strain WAg522 (De et al., 1999). This strain, along with the other test M. bovis mutants, was shown to be avirulent for guinea pigs following the subcutaneous inoculation (De et al., 1999). WAg520 was one of four nutritionally impaired strains of M. bovis selected from 440 M. bovis mutants produced by illegitimate recombination based upon their inability to grow in minimal medium (De et al., 1999). The antigen for this vaccine was an attenuated strain of M. bovis called WAg520, which cantains a deletion in a possible undecaprenol kinase gene (De et al., 1999). Duncan-Hartley Four to eight guinea pigs per group were vaccinated by subcutaneous injection with 10^5 CFU with one of the four impaired M. bovis strains or BCG. Ten animals were not vaccinated (control) (De et al., 1999). Significant differences in lung CFU were observed between vaccinated groups and WAg520 & WAg522 strains (De et al., 1999). WAg520 elicited 0.92, 3.83, and 2.47 log10 increases in lung, splenic, and liver tissues, respectively (Table I, (De et al., 1999)). Macroscopic lesions were decreased in WAg520 recipients versus both non-vaccinated (significant different) and BCG-vaccinated (not significantly different) animals (Table 2, (De et al., 1999)). No delayed-type hypersensitivity reactions observed prior to inoculation with mycobacteria (De et al., 1999). Authors were unable to determine if nutritionally-impaired mutants influenced the development of necrotizing lesions in the lungs (De et al., 1999). All animals were anesthetized eight weeks post-vaccination via intramuscular injection with 3 mg ketamine hydrochloride, followed by 10x2 CFU virulent M. bovis WAg201 introduced through mouth into the trachea via cannula. Three guinea pigs which were not challeged received 10^5 CFU of each of the four strains via subcutaneous innoculation (De et al., 1999). All animals were sacrificed 13 weeks post-vaccination. M. bovis WAg522 strain VO_0000557 Attenuated vaccine This strain, along with the other tested M. bovis mutants in (De et al., 1999), was shown to be avirulent in guinea pigs following subcutaneous inoculation. Furthermore, the groups treated with WAg522 and WAg520 strains both showed significant differences in lung CFU versus nonvaccinated groups. WAg522 is the second of four M. bovis mutants selected from 440 illegitimate recombinant M. bovis mutants via their inability to grow in minimal medium (De et al., 1999). The antigen for this vaccine was an attenuated strain of M. bovis called WAg522 (De et al., 1999). Duncan-Hartley Four to eight guinea pigs per group were vaccinated by subcutaneous injection with 10^5 CFU with one of the four impaired M. bovis strains or BCG. Ten animals were not vaccinated (control) (De et al., 1999). Significant differences in lung CFU were observed between vaccinated groups and WAg520 & WAg522 strains (De et al., 1999). WAg520 elicited 0.92, 3.83, and 2.47 log10 increases in lung, splenic, and liver tissues, respectively (see Table I, (De et al., 1999)). Macroscopic lesions were decreased in WAg520 recipients versus both non-vaccinated (significant different) and BCG-vaccinated (not significantly different) animals (see Table 2, (De et al., 1999)). No delayed-type hypersensitivity reactions observed prior to inoculation with mycobacteria (De et al., 1999). Authors were unable to determine if nutritionally-impaired mutants influenced the development of necrotizing lesions in the lungs (De et al., 1999). All animals were anesthetized eight weeks post-vaccination via intramuscular injection with 3 mg ketamine hydrochloride, followed by 10x2 CFU virulent M. bovis WAg201 introduced through mouth into the trachea via cannula. Three guinea pigs which were not challeged received 10^5 CFU of each of the four strains via subcutaneous innoculation (De et al., 1999). M. tuberculosis DNA vaccine (containing the ESAT-6, MPT-64, MPT-83, and KatG constructs) VO_0004363 DNA vaccine Research pJW4303 [Ref1534:Morris et al., 2000] Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction Vector pJW4303 encoded MPT-64 (Morris et al., 2000). DNA vaccine construction Vector pJW4303 encoded MPT-83 (Morris et al., 2000). DNA vaccine construction Vector pJW4303 encoded KatG (Morris et al., 2000). VO_0000286 The relative protection in the lung induced by the combination vaccine approached the BCG response in these experiments (Morris et al., 2000). M. tuberculosis DNA Vaccine APADNA priming and APAMVA boosting VO_0011478 Other Research pUMVC3 and poxvirus [Ref1186:Kumar et al., 2003]. Intradermal injection (i.d.) Intradermal injection (i.d.) Recombinant vector construction Animals were immunized intradermally with APADNA, 200 μg, given three times, 4 weeks apart (at weeks 0, 4, and 8), followed by APAMVA, 10^7 PFU, given 4 weeks after the third DNA (at 12 weeks); or BCG Guindy, 10^6 CFU, given one time only (at week 0) or control DNA, 200 μg, given three times, 4 weeks apart (at weeks 0, 4, and 8), followed by MVA wild type, 10^7 PFU, given 4 weeks after the third DNA (at week 12) (Kumar et al., 2003). A poxvirus recombinant expressing the Apa protein conferred a significant level of protective immunity in guinea pigs against a challenge dose of virulent M. tuberculosis (Kumar et al., 2003). Animals were challenged intramuscularly in the thigh muscle at 4 weeks after the last immunization with 2 × 10^5 viable M. tuberculosis strain NTI64719. This challenge dose was sufficient to cause measurable clinical illness in all control animals within a relatively short time frame of 6 weeks (Kumar et al., 2003). M. tuberculosis DNA vaccine DNAAg85A encoding a single immunogenic M.tb Ag VO_0004364 DNA vaccine Research pCDNA3.1 [Ref2263:Wang et al., 2004] intranasal immunization intranasal immunization DNA vaccine construction Vector pcDNA3.1 expressed a single immunogenic M.tb Ag (Ag85A) (Wang et al., 2004). VO_0000286 Systemic priming with two repeated DNAAg85A injections and respiratory mucosal boosting once with AdAg85A provided the most potent protection of all of the tested vaccines/regimens, including s.c. BCG vaccination both at 4 and 8 wk postchallenge, and this level of protection was also better than single i.n. AdAg85A vaccination at 8 wk postchallenge (Wang et al., 2004). M. tuberculosis DNA Vaccine encoding Ag85B Protein VO_0011410 DNA vaccine Research pRC [Ref1404:Palma et al., 2007] Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction C57BL/6 Mice at or between 7 and 8 weeks old were immunized. Fifty micrograms of plasmid, both DNA-85B or control vector, was injected intramuscularly (i.m.) in 50 μl PBS into the hind leg. Mice were immunized two or four times at 2 week intervals. As a positive control, a single dose of BCG (105 cfu) was injected s.c. (Palma et al., 2007). A significant reduction of the bacterial load was observed in mice receiving two administrations of the DNA-85B vaccine (DNAx2-immunized mice) with respect to unvaccinated naïve control or empty-plasmid DNA recipient mice, indicating protection by the vaccine. The degree of reduction of the mycobacterial burden was not significantly different from that conferred by BCG, although the immunization with BCG caused the highest nominal reduction of mycobacteria in the lungs (Palma et al., 2007). Four weeks after the last boost, mice were challenged intravenously (i.v.) in a lateral tail vein with 10^5 cfu of MTB H37Rv (Palma et al., 2007). M. tuberculosis DNA vaccine encoding Ag85B, MPT64 and MPT83 VO_0004362 DNA vaccine Research pJW4303 [Ref2262:Cai et al., 2005] Intramuscular injection (i.m.) IL-2 (Cai et al., 2005) Intramuscular injection (i.m.) DNA vaccine construction Vector pJW4303 expressed Ag85B of H37Rv (Cai et al., 2005). DNA vaccine construction Vector pJW4303 expressed MPT64 of H37Rv (Cai et al., 2005) DNA vaccine construction Vector pJW4303 expressed MPT83 of H37Rv (Cai et al., 2005). VO_0000286 Combined DNA vaccines including Ag85B, MPT64, and MPT83 formulated in IL-2 provided an efficient method for generating strong and sustained protective immunity in the spleen and lungs of experimental mice following i.v. challenge with M. tuberculosis H37Rv (Cai et al., 2005). M. tuberculosis DNA Vaccine encoding KatG VO_0004058 DNA vaccine Research The eukaryotic expression vector pJW4303 Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction KatG protein was amplified from M. tuberculosis H37Rv chromosomal DNA by PCR, and then cloned into the eukaryotic expression vector pJW4303 (Li et al., 1999). C57BL/6 The C57BL/6 mice (five mice per group) were vaccinated intramuscularly into the thigh three times at 3-week intervals, with 100 μg of plasmid DNA per injection. Control mice were vaccinated with 10^6 CFU of BCG Pasteur injected subcutaneously (Li et al., 1999). Vaccination with the KatG resulted in a 75 to 85% drop in number of lung bacteria. Moreover, mice immunized with the KatG TPA-positive constructs showed significantly decreased dissemination of the lung infection to the spleen (Li et al., 1999). The mice were challenged by the aerosol route about 17 to 21 days after receiving the final DNA vaccine inoculation and 6 weeks after receiving the BCG vaccination with M. tuberculosis Erdman (Li et al., 1999). M. tuberculosis DNA vaccine ESAT-6 VO_0004035 DNA vaccine Research pJW4303 [Ref1534:Morris et al., 2000] Intramuscular injection (i.m.) Intramuscular injection (i.m.) Immunization with a multivalent combination DNA vaccine (containing the ESAT-6, MPT-64, MPT-63, and KatG constructs) generated immune responses that indicated an absence of antigenic competition since antigen-specific cell-mediated and humoral responses were detected to each component of the mixture (Morris et al., 2000). VO_0000286 Prior to vaccination, mice were challenged with M. tuberculosis. Among the single DNA vaccines that were tested, the ESAT-6 construct consistently evoked the best protective responses. Compared with BCG, which on a relative scale elicited a protective value of 1, ESAT-6 elicited a protective value of 0.61 (Morris et al., 2000). M. tuberculosis DNA vaccine pAK4-sod VO_0004123 DNA vaccine pAK4 [Ref549:Khera et al., 2005] A study compared DNA vaccines expressing mycobacterial antigens ESAT-6 (Rv3875), α-crystallin (Rv2031c) and superoxide dismutase A (Rv3846) in Balb/c mice and guinea pigs. The DNA vaccine expression sodA was found to offer maximum protection (Khera et al., 2005). None PCR amplified using gene specific primers containing Bgl II overhangs and M. tuberculosis genomic DNA as template. The blunt end PCR amplicon was cloned into EcoR V digested pLitmus-38, and then subcloned into plasmids pAK3 and pAK4 resulting in pAK3-sod and pAK4-sod. While both pAK3 and pAK4 are the eukaryotic expression vectors, pAK4 contains more CpG motifs. pAK4 also carries the ampicillin resistance gene carrying two immunostimulatory sequences. pAK3 is devoid of this ampicillin gene (Khera et al., 2005). mycobacterial antigen superoxide dismutase A (Rv3846). DNA vaccine preparation The gene sodA was subcloned into the expression vectors pAK3 and pAK4 (Khera et al., 2005). Immunization of mice with the DNA vaccines expressing superoxide dismutase A resulted in markedly higher levels of IFN-γ as compared to the levels of IL-10. The levels of IFN-γ in supernatants from pAK3-sod and pAK4-sod immunized mice increased 2.9 and 9.1 folds compared to the levels of IFN-γ in culture supernatant of mice immunized with pAK-3 and pAK-4, respectively. Approximately 3-fold increase in IFN-γ levels in pAK4-sod immunized mice in comparison to pAK3-sod immunized mice showed that the extra CpG motif activated Th1 subtype of host immunity. Though expression of SOD antigen increases IL-10 levels, the extra CpG motifs in the vector backbone decreases the concentration of IL-10 in pAK4-sod immunized mice in comparison to pAK3-sod immunized mice (Khera et al., 2005). Balb/c mice Mice (in groups of six each) were immunized with either the pAK3 or pAK4 vector controls or DNA vaccine candidates. Three doses of 100 μg of plasmid DNA were administered intramuscularly at 3-week intervals. Following a rest period of 3 weeks after the last dose, mice were euthanized, and antigen specific cell mediated and humoral immune responses were evaluated (Khera et al., 2005). No challenge experiment was performed in mice. Guinea pigs were used for protection assay for this vaccine. The levels of IFN-γ in supernatants from pAK3-sod and pAK4-sod immunized mice significantly increased compared to the plasmid vector control groups (Khera et al., 2005). Compared to the plasmid pAK3 and pAK4 controls, expression of the SODA antigen increased IL-10 levels in DNA vaccine pAK3-sod and pAK4-sod immunized mice. The extra CpG motifs in the pAK4 plasmid decreases the IL-10 level in pAK4-sod immunized mice compared to pAK3-sod immunized mice (Khera et al., 2005). Outbred guinea pigs of the Duncan Hartley strain DNA vaccine expressing superoxide dismutase imparted the maximum protection as observed by a 50 and 10 folds reduction in bacillary load in spleens and lungs, respectively, in comparison to immunization with vector control. Among the animals vaccinated with various DNA vaccines, pAK4-sod immunized guinea pigs exhibited the minimal granuloma (Khera et al., 2005). Guinea pigs (in groups of six) were immunized intramuscularly with three doses (of 100 μg each) of pAK4, pAK4-E6 expressing ESAT-6, pAK4-αcry expressing α-crystallin, or pAK4-sod at 3 weeks intervals. Four weeks after the last booster, guinea pigs were subcutaneously challenged with 1 × 10^5 CFU of M. tuberculosis H37Rv. Guinea pigs were euthanised 4 weeks after challenge (Khera et al., 2005). M. tuberculosis DNA vaccine pcDNA3.1+/Ag85A DNA encoding Ag85A VO_0004356 DNA vaccine Research pcDNA3.1 [Ref2249:Wang et al., 2010] Intramuscular injection (i.m.) Liposomes (Wang et al., 2010) Intramuscular injection (i.m.) DNA vaccine construction Vector pcDNA3.1 expressed Mycobacterium antigen 85A (Ag85A) (Wang et al., 2010). Orally administrated liposomal-pcDNA3.1+/Ag85A DNA was efficiently incorporated into mucosal epithelium of the small intestine, Peyer's patches (PP). This initiated a Ag85A-specific Th1 dominant immune response, as evidenced by increased secretion of IL-2, IFN-γ, and no change of IL-4 (Wang et al., 2010) VO_0000286 Liposomal-pcDNA3.1+/Ag85A DNA significantly increased the protection by giving a markedly reduction of TB burden in the lung, demonstrating that the TB-specific immune responses elicited by oral administration of liposomal-pcDNA3.1+/Ag85A DNA led to an enhanced host defense against TB infection. (Wang et al., 2010) M. tuberculosis HBHA Protein Vaccine VO_0004059 Subunit vaccine Research subcutaneous injection DDA-MPL subcutaneous injection HBHA Recombinant protein preparation C57BL/6 were vaccinated subcutaneously three times with 5 μg of either HPLC-purified HBHA (nHBHA) or recombinant HBHA emulsified in 150 μg of dimethyldioctadecylammonium bromide (DDA) (Acros Organics, Morris Plains, N.J.)-monophosphoryl lipid A (MPL) adjuvant or with the adjuvant combination alone (Parra et al., 2004). The HBHA-containing vaccine gave a approximately 0.7-log reduction in CFU in both mouse lungs and spleens compared to adjuvant controls 28 days following challenge (Parra et al., 2004). Mice were challenged with aerosolized M. tuberculosis Erdman (200 CFU) 4 weeks following the final immunization (Parra et al., 2004). There was a ~10-fold increase in the amount of IFN-γ released in the ex vivo assay by spleen cells from HBHA-immunized mice compared to cells from naïve mice or mice immunized with only adjuvant, which was a significantly higher amount. Splenocytes from immunized mice were incubated with murine BMMΦ infected with M. bovis BCG for 72 hours. Lymph node-derived lymphocytes from HBHA-vaccinated mice released approximately eightfold more IFN-γ in the presence of HBHA-stimulated macrophages than cells from naïve or adjuvant control mice released, which was also significant (Parra et al., 2004). M. tuberculosis Mtb72F Protein Subunit Vaccine VO_0011412 Subunit vaccine Research Intramuscular injection (i.m.) AS02A (Skeiky et al., 2004). Intramuscular injection (i.m.) Recombinant protein preparation C57/BL6 Mice were immunized three times (3 wk apart) with 8 µg of rMtb72F formulated with the adjuvants AS02A. For protein formulations, the required immunization dose of rMtb72F (typically 8 µg) was brought up to either 50 or 43 µl with 1x PBS (pH 6.8) and mixed with 57 µl of AS02A. Mice were injected with a total volume of 100 µl/mouse via the i.m. (tibialis) route with 50 µl/leg. Positive control mice were immunized with BCG (5 x 10^4 CFU) in the base of the tail (once), and negative control animals were injected with saline, adjuvant alone, or DNA vector (Skeiky et al., 2004). Immunization of mice with Mtb72F protein formulated in the adjuvant AS02A resulted in the elicitation of a moderate IFN-gamma response and a weak CD8(+) T cell response to Mtb32c. Mtb72F immunization resulted in the protection of C57BL/6 mice against aerosol challenge with a virulent strain of M. tuberculosis(Skeiky et al., 2004). Thirty days after the last immunization, mice were challenged by low dose aerosol exposure with M. tuberculosis H37Rv strain (Skeiky et al., 2004). M. tuberculosis phoP mutant SO2 M. tuberculosis phoP mutant strain VO_0000588 Live attenuated vaccine The phoP transcription factor is part of the two-component regulatory signal transduction system component which recently was linked to regulation of complex mycobacterial lipids implicated in the virulence of M. tuberculosis, as well as to multidrug resistance occurrences in a clinical isolate (Martin et al., 2006). (Perez et al., 2001) established the selection of phoP as a vaccine candidate by showing that the mutant SO2 strain shows impaired multiplication within cultured macrophages and in vivo infection models (Martin et al., 2006). Rabbits were treated with four doses of phoP prior to isolating polyclonal antibodies against phoP. Anti-phoP and monoclonal antibodies against ESAT-6 were obtained. Horseradish peroxidase-labelled goat anti-rabbit antibodies served as secondary antibodies (Martin et al., 2006). Mycobacterial cell-free protein extracts from M. tuberculosis were prepared, filtered, and precipitated with 45% (w/v) ammonium sulphate (Martin et al., 2006). Gene mutation The mutant strain was constructed by a single gene (phoP) disruption of the parental M. tuberculosis MT103 strain. PhoP has shown involvement in the regulation of complex mycobacterial lipids implicated in the virulence of M. tuberculosis (Martin et al., 2006). Balb/C mice Groups of four Balb/C mice were vaccinated with either 2.5x10^3 CFUs of SO2 or 8x10^3 CFUs of M. bovis BCG and later sacrificed at 7, 14, 21, 28, 45, and 60 days post-vaccination (Martin et al., 2006). Vaccination with M. tuberculosis SO2 induced a significantly higher proportion of CD4+/IFN-gamma+ producing cells after 45 days of vaccination when compared with BCG (Martin et al., 2006). The proportion of CD8+/IFN-gamma+ producing cells was consistently higher in the M. tuberculosis SO2group. SO2 strain and BCG both conferred significant levels of protection compared to saline controls, with reductions of 1.5 and 1.3 log10 reductions in cfu counts in lung and spleen (Martin et al., 2006). However, To asses protective efficacy of SO2 in Balc/c mice, mice were challenged by intravenous route with 2.5x10^5 CFU M. tuberculosis H37Rv at week 8 and sacrificed four weeks later. Vaccination with M. tuberculosis SO2 induced a significantly higher proportion of CD4+/IFN-γ+ producing cells in mice 45 days after vaccination when compared with BCG (Martin et al., 2006). Groups of six guinea pigs were used in two procedures. Guinea pigs were vaccinated subcutaneously with 250μl of 5x10^4 CFU BCG (Pasteur) or SO2 in both the low-dose and high-dose challenge experiments. Saline was also used in the low-dose experiments. A significant difference was observed between non-vaccinated and vaccinated (both BCG and SO2-recipient) groups receiving low-dose treatment, whereas no significant difference was evident between the BCG and SO2 groups. In high dose challenge, all non-vaccinated and BCG-vaccinated were euthanized according to set humane end-point, while all SO2-vaccinated groups showed significantly longer survival than BCG groups and saline-treated groups. Pulmonary disease most evident in non-vaccinated guinea pigs. Consolidation was significantly less in the SO2-vaccinated groups versus non-vaccinated and BCG-vaccinated groups. "M. tuberculosis SO2 strain was superior to BCG in conferring enhanced survival to infected guinea pigs, reduction in the severity of the disease in the lung, and dissemination of infection to the spleen" (Martin et al., 2006). SO2-treated guinea pigs gained weight. No visible or clinical signs of disease after challenge. In the low dose challenge, guinea pigs were aerosol challenged at 12 weeks using a Henderson apparatus and 2x10^6 CFU/ml M. tuberculosis water suspension to give estimated inhaled dose of 10-50 CFU/lung. In high dose challenge, guinea pigs were aerosol challenged at 10 weeks with 5x10^7 CFU/ml M.tuberculosis in water suspension to give approimately 500 CFUs to the lungs. Animals were killed by peritoneal overdose of sodium pentabarbitone (Chandra et al., 2006). CB-17/lcr lco SCID specific pathogen free (spf) No vaccination was employed here. Purpose of experiment is to assess survival of SCID mice following infection with either SO2 or BCG (Martin et al., 2006). All SCID mice infected with SO2 strain survived for >245 days, whereas those infected with M. tuberculosis MT103 and SO2-pSO5 died within 62 days of infection(Martin et al., 2006). Ten mice per experimental group were infected with 200μl PBS containing 2x10^5, 2x10^4, or 2x10^3 viable BCG Pasteur, or 5.4x10^6, 5.4x10^5, or 5.4x10^4 viable SO2. Aerosol infection was also used to nebulize with 7 ml of M. tuberculosis suspension, providing 20 viable bacilli within the lungs. Survival times were determined using Mantel-Haenszel test (Martin et al., 2006). M. tuberculosis secA2 mutant VO_0000564 Live, attenuated vaccine The secA2 gene encodes a component of a virulence-associated bacterial protein secretion system and is required for prevention of apoptosis in macrophages infected with M. tuberculosis. The apoptotic response is related to secA2-dependent secretion of mycobacterial superoxide dismutase A (SodA). M. tuberculosis H37Rv with deleted secA2 induces apoptosis, as indicated by TUNEL and polycaspase activation in mouse and human macrophages (Hinchey et al., 2007). Extrinsic and intrinsic caspase pathways were linked to initiation of apoptosis in the secA2-deleted mutants via caspase 8 and 9 activity. The secretion of SodA appears to be important for the inhibition of apoptosis by secA2-dependent mechanisms. Furthermore, Three virulent strains (M. tuberculosis H37Rv, HN878, and ΔsecA2 mutants) were obtained and grown on Middlebrook 7H9 broth or 7H10 agar containing 10% (vol/vol) oleic acid-albumin-dextrose-catalase, 0.5% glycerol, and 0.05% (vol/vol) Tween-80. Recombinant strains were selected using ampicillin, hygromycin, and kanamycin. secA2 transformation into M. tuberculosis The secA2 gene was amplified by PCR from virulent H37Rv genomic DNA, cloned into a pGEM vector, and later excised as a fragment and cloned into pMV361 (Hinchey et al., 2007). OT-1 T cells infected with H37Rv, H37Rv-OVA, and ΔsecA2-OVA showed negligible, modest, and massive increases in proliferation of transferred T cells. SecA2 addition can restore OT-1 T cell priming activities in the mutants. Furthermore, SIINFEKL epitope is produced in all mutants in an immunogenic form. Additional evidence suggested that tight linkage exists among CD8+ T cell priming, loss of SodA secretion, and blocked apoptosis (Hinchey et al., 2007). B6.PL (Thy1.1+), SIINFEKL/H-2K^b-reactive TCR-transgenic OT-1 Donor splenocytes from Rag1 deficient OT1 TCR-transgenic mice were isolated and labeled with 20 μg CFSE and washed. Aliquots containing either 3x10^5 or 1x10^7 labeled cells were injected into lateral tail vein prior to subcutaneous or i.v. infection with 1x10^8 CFU M. tuberculosis ΔsecA2-OVA or control bacteria strains. Splenocytes were harvested and stained 5-7 days post-infection and analyzed by flow cytometry to determine percentage of undivided (high-CFSE) cells in the Thy1.2+ population (Hinchey et al., 2007). No challenge was implemented here. Procedure was designed to visualize and better understand the priming of MHC class I restricted CD8+ T cells with SIINFEKL fusion protein, as well as understand the relationship between SodA secretion, apoptosis, and secA2 (Hinchey et al., 2007). Infection with H37Rv, H37Rv-OVA or ΔRD1-OVA induced background levels of IFN-gamma spot-forming cells while the ΔsecA2-OVA infection induces a 10-fold increase of similar spots above the background levels. Additional evidence from an in vivo CTL assay for SIINFEKL -induced CD8+ T cell activity strengthened the claim that enhanced T cell priming in the sec2A deletion mutants is restricted to CD8+ T cell compartment (Hinchey et al., 2007). C57BL/6 Mice received 5x10^5 OT-1 cells one day before infecting subcutaneously with either 1x10^6 CFU H37Rv-OVA or ΔsecA2-OVA. Animals were sacrificed at 2, 4, or 20 weeks prior to splenic isolation and antibody labeling. Lympocyte subpopulations (CD44high,CD62high; CD44highCD62low) were quantified and separated using flow cytometry (Hinchey et al., 2007). No challenge was introduced in this protocol. Purpose of this experiment was to analyze memory T cell populations and abundance within the native splenic environment, and to determine the extent of antigen transmission to dendritic cells and enhanced CD8+ T cell priming (Hinchey et al., 2007). Mild inflammation including small/compact granulomas containing lymphocytes were observed for animals vaccinated with BCG or ΔsecA2 (Hinchey et al., 2007). C57BL/6 Mice were vaccinated subcutaneously with 1x10^6 CFUs of either M. tuberculosis ΔsecA2 or M. bovis BCG-Pasteur. Substantial growth of HN878 observed in lungs and spleens within one month of challenge (Hinchey et al., 2007), whereas both M. tuberculosis ΔsecA2 or M. bovis BCG-Pasteur showed decreased loads at the same interval. Protection by ΔsecA2 was significantly greater than in animals infected with M. bovis BCG-Pasteur at one month post-challenge (Hinchey et al., 2007). Furthermore, the protection offered by ΔsecA2 was still significant after five months post-challenge despite non-significant BCG protection at the same interval. Mice were challenged via aerosol route two months post-vaccination using GlasCol inhalation chamber. Approximately 50-100 CFUs of either M. tuberculosis Beijing/W (HN878) or Erdman strains were administered using this approach. Mice were sacrificed at 1, 3, and 5 months post-challenge prior to isolation of lung and spleen. Mice infected with the Erdman strain were observed daily until euthanization in a second survival study (Hinchey et al., 2007). Minor fibrosis observed for in ΔsecA2-immunized animals at 2 months post-challenge (Hinchey et al., 2007). Outbred Hartley guinea pigs Guinea pigs were vaccinated intradermally with 1x10^3 M. tuberculosis ΔsecA2 or BCG-Pasteur (Hinchey et al., 2007). Lower CFUs observed in ΔsecA2-immunized animals versus BCG and control animals (Hinchey et al., 2007). The ΔsecA2-immunized animals exhibited significant decreases (p<.01) in lung, splenic, and mediastinal lymph node CFUs versus control animals. Notable differences in CFU counts and organ size in the mediastinal lymph nodes were observed in only the M. tuberculosis ΔsecA2-innoculated animals, including only minor fibrosis at two months post-challenge (Hinchey et al., 2007). Animals were challenged six weeks post-vaccination via aerosol route with 10-30CFUs of M. tuberculosis H37Rv strains (Madison chamber). Spleen, lung, and lymph node tissues were isolated at either 1 or 2 months post-challenge. (Hinchey et al., 2007). Guinea pigs were vaccinated and challenged for CFU determination and histopathology comparison between different attenuated strains of M. tuberculosis and M. bovis. The results from the guinea pig study suggest that the . M. tuberculosis ΔsecA2 exceeds the efficacy of BCG in several aspects and may be a viable vaccine candidate (Hinchey et al., 2007). M.S-Δesx-3-IKEPLUS (M. tuberculosis) VO_0004689 Recombinant vector vaccine Research Intramuscular injection (i.m.) (Sweeney et al., 2011) Intramuscular injection (i.m.) M72/AS01E Subunit vaccine Clinical trial Intramuscular injection (i.m.) ASO1E adjuvant is use in this vaccine (Tait et al., 2019). The TB proteins are genetically fused into one protein (M72), then purified and formulated with the AS01E adjuvant system (Tait et al., 2019). Intramuscular injection (i.m.) The vaccine contains M72 fusion protein, which is made from two Mycobacterium tuberculosis antigens: Mtb32A and Mtb39A (Tait et al., 2019). Fusion Protein This is use in fusion protein preparation (Tait et al., 2019). Fusion Protein This is use in fusion protein preparation (Tait et al., 2019). The vaccine induces a strong immune response characterized by activation of antigen-specific CD4⁺ and CD8⁺ T cells, increased production of cytokines such as IFN-γ, TNF-α, and IL-2, and the development of long-lasting memory T cells, along with enhanced antibody production (Tait et al., 2019). Participants are immunized 2 doses spaced by one month (Tait et al., 2019). Injection site reactions and flu like symptoms (Tait et al., 2019). Mtb72f fusion protein VO_0004108 Fusion protein with adjuvant Mice were immunized three times (at 3 week intervals) with 8μg of recombinant Mtb72F (rMtb72F) (Skeiky et al., 2004). Adjuvants included AS02A or AS01B (Skeiky et al., 2004). See genetic engineering section for details regarding construction of rMtb72F. Vaccines included rMtb72F combined with either of two adjuvants, or Mtb72F alone (Skeiky et al., 2004). Immunization dose was either 50 or 43 μl with 1x PBS (pH 6.8) mixed with 50 μl of AS01B or 57 μl of AS02A, respectively (Skeiky et al., 2004). Fusion protein from co-expressed ORFs Two open reading frames for M. tuberculosis antigenic proteins Mtb32 and Mtb39 (Rv0125 and Rv1196, respectively) were combined and expressed as a single recombinant polyprotein of size 72kDa. Each ORF was PCR amplified and subcloned into plasmids prior to transformation into E. coli. Correct inserts and orientation were identified by restriction digests and DNA sequencing. The final Mtb72f fusion protein acts as an activator of IFN-gamma in CD4 and CD8 T cells (Skeiky et al., 2004). Fusion protein from co-expressed ORFs Two open reading frames for M. tuberculosis antigenic proteins Mtb32 and Mtb39 (Rv0125 and Rv1196, respectively) were combined and expressed as a single recombinant polyprotein of size 72kDa. Each ORF was PCR amplified and subcloned into plasmids prior to transformation into E. coli. Correct inserts and orientation were identified by restriction digests and DNA sequencing. The final Mtb72f fusion protein acts as an activator of IFN-gamma in CD4 and CD8 T cells (Skeiky et al., 2004). C57/BL6 female mice Mice were age-matched (4-6 weeks) per experiment, and immunized 3 times (2 wk apart) with 8μg of rMtb72f and selected adjuvants. Immunization dose was increased from 8μg using 50 or 43 μl of PBS with 50 or 57 μl of AS01B or AS02A adjuvants. In total, 100 μl of vaccine was injected via i.m. (tibialis) route with 50 μl/leg (Skeiky et al., 2004). Additional culturing protocols were included for mononuclear and splenic cells (see (Skeiky et al., 2004)). Mice immunized with 5x10^4BCG in base of tail served as positive controls. Negative controls were injected with saline, adjuvant alone, or DNA vector. The extent of protection with Mtb72F represents the longest documented survival end point reported for any defined subunit vaccine (~40% survival after 80 weeks post-challenge) (Skeiky et al., 2004). Mice immunized with DNA developed an Mtb72f Ab response of the IgG2a but not IgG1 subclasses which was directed against N-terminal (Mtb32c) molecules. Strong IFN-gamma responses were elicited in response to rMtb72F, rMtb32c, Mtb39, and PPD treatments. Mice immunized with rMtb72f and either adjuvant mounted strong IgG1 and IgG2a responses against Mtb72F. The AS01B-Mtb72F formulation elicited robust CD8+ T cell responses (directed against Mtb32c), whereas the AS02A-Mtb72F formulation were weaker than the AS01B formulation. However, both adjuvants when combined with Mtb72f could elicit ~0.6 log reduction in bacteria burden of mouse lungs. The Mtb72F-DNA provided 0.7-1.0 log reduction in bacterial load, which is comparable to BCG results (Skeiky et al., 2004). Thirty days after the last immunization, mice were challenged by low-dose aerosol exposure (50-100 bacteria in lungs) with M. tuberculosis H37Rv strain. Mice were euthanized four weeks later prior to lung and spleen homogenate preparation. Similar to mouse protocol, the guinea pigs were immunized three times (3 weeks apart) with 200μg Mtb72F-DNA with 250μl of 1xPBS @ pH 7.0, 20-μg dose of rMtb72f, or a mixture of these components in a total 250uL dose containing AS02A. Guinea pigs were immunized with 125μl of final formula in the leg (Skeiky et al., 2004). BCG administered to some animals via intradermal route. *** Evidence suggests that the protection can last for > 1 year (Skeiky et al., 2004). After 30 wk postchallenge, four of five guinea pigs immunized with rMtb72F and three of five immunized with Mtb72F-DNA were alive, whereas after 70 weeks, two of five in DNA-immunized groups were still alive. Animals were challenged with virulent H37Rv strain via aerosol route after 13 weeks, with nebulizer providing 20-50 bacteria into the lungs (Skeiky et al., 2004). MVA/IL-15/5Mtb vaccine VO_0004263 Recombinant vector vaccine Research subcutaneous injection subcutaneous injection A recombinant modified vaccinia Ankara (MVA) construct (MVA/IL-15/5Mtb) which overexpresses five Mycobacterium tuberculosis antigens (antigen 85A, antigen 85B, ESAT6, HSP60, and Mtb39) (Kolibab et al., 2010). Recombinant vector construction Recombinant vector construction Recombinant vector construction C57BL/6 C57BL/6 female mice were vaccinated once subcutaneously with 10^6 CFU of BCG Pasteur 6 weeks before challenge. For the wild-type MVA vector (as a control) or the MVA/IL-15/5Mtb vaccine, mice were administered two doses of 5 × 10^7 PFU subcutaneously at 1 month apart (Kolibab et al., 2010). Mice vaccinated with the MVA/IL-15/5Mtb construct had significant protection at 2 and 12 months post challenge. At 12 months postvaccination, highly persistent protective responses were seen in the lungs of mice immunized with BCG vaccine or our MVA/IL-15/5Mtb construct (Kolibab et al., 2010). Five mice from each group were aerogenically challenged with M. tuberculosis Erdman K1 (Trudeau Mycobacterial Culture Collection) suspended in phosphate-buffered saline (PBS) at a concentration known to deliver 200 CFU in the lungs over a 30-min exposure in a Middlebrook chamber (Kolibab et al., 2010). Two and sixteen months post vaccination with MVA/IL-15/5Mtb, IFN-gamma was significantly upregulated in mice as compared to naive control mice in lungs. However, it was significantly down-regulated as compared to mice vaccinated with BCG (Kolibab et al., 2010). Two months post vaccination with MVA/IL-15/5Mtb, IL12b (IL12 p40 subunit) was significantly upregulated in mice lungs as compared to naive control mice (Kolibab et al., 2010). Two and sixteen months post vaccination with MVA/IL-15/5Mtb, IL-17F was significantly upregulated in mice lungs as compared to naive control mice. However, it was significantly down-regulated as compared to mice vaccinated with BCG (Kolibab et al., 2010). Two months post vaccination with MVA/IL-15/5Mtb, IL-27 beta (Ebi3) was significantly upregulated in mice lungs as compared to naive control mice Two and sixteen months post vaccination with MVA/IL-15/5Mtb, Cxcl9 was significantly upregulated in mice lungs as compared to naive control mice. However, it was significantly down-regulated as compared to mice vaccinated with BCG 16 months post vaccination (Kolibab et al., 2010). Two and sixteen months post vaccination with MVA/IL-15/5Mtb, Cxcl10 was significantly upregulated in mice lungs as compared to naive control mice. However, it was significantly down-regulated as compared to mice vaccinated with BCG 16 months post vaccination (Kolibab et al., 2010). Two months post vaccination with MVA/IL-15/5Mtb, Cxcl11 was significantly upregulated in mice lungs as compared to naive control mice. However, it was significantly down-regulated as compared to mice vaccinated with BCG 16 months post vaccination (Kolibab et al., 2010). IL-15 was used as an adjuvant for the modified vaccinia Ankara vaccine (MVA). Homologous prime/boost studies showed that the MVA/IL-15/5Mtb vaccine induced moderate but highly persistent protective immune responses for at least 16 months after the initial vaccination and that the interval between the prime and boost did not significantly alter vaccine-induced antituberculosis protective immunity. Organ (lung or spleen) bacterial burdens (CFU) in the MVA/IL-15/5Mtb vaccine-immunized mice were greater than those in BCG vaccinated animals, but less than those in mice vaccinated with MVA alone. MVA/IL-15/5Mtb also induced protection that was equivalent to BCG, whereas MVA alone did not induce protective immunity (Kolibab et al., 2010). MVA85A prime and BCG boost VO_0004106 Subunit vaccine MVA is avirulent in normal and immunocompromised animals and has shown no significant side-effects in 120,000 vaccinated humans. Recombinant fowlpox FP9 cannot replicate in mammalian cells and is not pathogenic in mammals or birds (Vordermeier et al., 2004). Mycobacterial mycolyl transferase Ag85A (Vordermeier et al., 2004). Recombinant virus expressing Ag85A Prime-boost protocols, using recombinant MVA and BCG in combination (groups 1-3), resulted in significantly higher frequencies of Ag85-specific IFN-gamma-secreting cells than the two viral vectors used in combination (P=0.0055), or BCG used alone (groups 2 and 3, P=0.04) (Vordermeier et al., 2004). Friesians, 6-mo. old Groups of four calves were subjected to one of five schedules in the first experiment: Group 1: MVA85A was administered at week 0, followed by BCG at week 9. Group 2: BCG Pasteur was administered at week 0, followed by MVA85A at week 9. Group 3: BCG administered at week 0, FP85A (fowlpox) administered at week 8, followed by MVA85A at week 10. Group 4: FP85A administered at weeks 0 and 3, followed by MVA85A at week 8. Group 5: (treatments reversed versus group 4): MVA85A administered at weeks 0 and 3, followed by FP85A at week 8 (Vordermeier et al., 2004). A 10^6 CFU dose of BCG was injected subcutaneously in side of neck with 1 ml PBS. MVA8A and FP85A doses were each administered as 10^9 plaque-forming units (PFUs) in PBS in a total 0.1 mL injection volume at each injection site. Animals skin tested with SICCT 14 weeks post-first vaccination (Vordermeier et al., 2004). In the second experiment, six calves were vaccinated s.c. with 10^6 CFU BCG Pasteur in 1 ml PBS. Three calves each were revaccinated with either a 10^9 PFU dose of MVA85 (intradermal) or 10^6 CFU dose of BCG Pasteur, 5 weeks later (Vordermeier et al., 2004). Mycobacterium tuberculosis DeltasigF mutant vaccine Live, attenuated vaccine Research Intradermal injection (i.d.) Intradermal injection (i.d.) Gene mutation Mycobacterium tuberculosis drrC mutant vaccine VO_0002780 Live, attenuated vaccine Research Intravenous injection (i.v.) Intravenous injection (i.v.) Gene mutation This drrC mutant is from Mycobacterium tuberculosis (Pinto et al., 2004). A drrC mutant is highly attenuated in mice (Pinto et al., 2004). A drrC mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Pinto et al., 2004). The drrC− strain was able to induce splenic anti–M. tuberculosis IFN-γ–secreting T cells responsive to CFP in mice to a level equal to that induced by M. tuberculosis infection and much greater than the amount induced by BCG and control mice. The drrC− mutant was also able to generate high numbers of IFN-γ–secreting cells that recognize the M. tuberculosis ESAT-6 antigen, which BCG and control mice could not do. IFN-gamma levels in both cases were comparable to levels induced by the wild type. This effect began 4 weeks after infection and was sustained long-term (24 weeks after infection) (Pinto et al., 2004). Mycobacterium tuberculosis fadD26 mutant vaccine VO_0002781 Live, attenuated vaccine Research Intratracheal immunization Intratracheal immunization Gene mutation This fadD26 mutant is from Mycobacterium tuberculosis (Infante et al., 2005). A fadD26 mutant is attenuated in mice (Infante et al., 2005). A fadD26 mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Infante et al., 2005). The fadD26 mutant induced a progressive increase in IFN-γ expression in lung homogenates of mice, peaking at day 60, and 2 months later it had decreased by only 20% from its maximal level. However, it induced significantly lower expression than that induced by the wild type pathogen (MT-103) from days 1-21 post infection (Infante et al., 2005). A fadD26 mutant induced a significantly lower and stable IL-4 expression as compared to the wild type (MT103) during the first month of infection in mice, followed by an increase of 50% at days 60 and 120. IL-4 levels of a fadD26 mutant-vaccinated mice were significantly lower than those infected with the wild type on days 3, 7, 21, 28, and 60 post infection in lung homogenates (Infante et al., 2005). The fadD26 mutant induced stable TNF-α expression in mice, significantly lower than that induced by MT-103 (WT strain) after 1 week of infection. In lung homogenates, fadD26 mutant vaccination induced significantly lower TNF-alpha levels on days 7, 14, 28, and 60 as compared to mice vaccinated with the wild type (Infante et al., 2005). Mice infected with the fadD26 mutant showed significant higher iNOS expression than the parent strain in lung homogenates days 21, 28 and 60 post infection. Results showed that the fadD26 mutant is also more efficient than its parental strain at inducing iNOS expression (Infante et al., 2005). Mycobacterium tuberculosis leuD mutant vaccine VO_0002782 Live, attenuated vaccine Research Intravenous injection (i.v.) Intravenous injection (i.v.) Gene mutation This leuD mutant is from Mycobacterium tuberculosis (Hondalus et al., 2000). A leuD mutant is attenuated in mice (Hondalus et al., 2000). A leuD mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Hondalus et al., 2000). Mycobacterium tuberculosis lysA/secA2 mutant vaccine VO_0002783 Live, attenuated vaccine Research subcutaneous injection subcutaneous injection Gene mutation This lysA/secA2 mutant is from Mycobacterium tuberculosis (Hinchey et al., 2011). Gene mutation This lysA/secA2 mutant is from Mycobacterium tuberculosis (Hinchey et al., 2011). A lysA/secA2 mutant is highly attenuated in mice (Hinchey et al., 2011). A lysA/secA2 mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Hinchey et al., 2011). Mycobacterium tuberculosis mce-2 mutant vaccine VO_0004279 Live, attenuated vaccine Research subcutaneous injection subcutaneous injection Gene mutation This mce-2 mutant is from Mycobacterium tuberculosis (Aguilar et al., 2006). Gene mutation This mce-2/mce-3 mutant is from Mycobacterium tuberculosis (Aguilar et al., 2006). A mce-2 mutant is attenuated in mice (Aguilar et al., 2006). A mce-2 mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Aguilar et al., 2006). Mutant mce-2 induced a lower but progressive expression of IFN-γ than the parent strain in mice, peaking at day 120 post-infection. The IFN-gamma response in lungs was significantly lower in mice immunized with mce-2 mutants on days 1, 3, 7, 14, and 21 days post infection as compared to mice infected with the wild type (Aguilar et al., 2006). The mce-2 mutant maintained low IL-4 expression until day 60 post-infection, showing slight increase at day 120. The expression of IL-4 was higher in the lungs of mice infected with the parental strain. Infection with the wild type strain induced significantly more IL-4 than that induced by immunization with the mutant on days 21, 28, and 60 post infection (Aguilar et al., 2006). Mycobacterium tuberculosis mce-3 mutant vaccine VO_0004288 Live, attenuated vaccine Research subcutaneous injection subcutaneous injection Gene mutation This mce-3 mutant is from Mycobacterium tuberculosis (Aguilar et al., 2006). A mce-3 mutant is attenuated in mice (Aguilar et al., 2006). A mce-3 mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Aguilar et al., 2006). The mce-3 mutant induced a lower but progressive expression of IFN-γ than the parent strain in mice, peaking at day 120 post-infection. The IFN-gamma response in lungs was significantly lower in mice immunized with mce-3 mutants on days 1, 3, 7, 14, and 21 days post infection as compared to mice infected with the wild type (Aguilar et al., 2006). The mce-3 mutant maintained low IL-4 expression until day 60 post-infection, showing slight increase at day 120. The expression of IL-4 was higher in the lungs of mice infected with the parental strain. Infection with the wild type strain induced significantly more IL-4 than that induced by immunization with the mutant on days 21, 28, and 60 post infection (Aguilar et al., 2006). Mycobacterium tuberculosis panCD mutant vaccine VO_0002786 Live, attenuated vaccine Research subcutaneous injection subcutaneous injection Gene mutation This panCD mutant is from Mycobacterium tuberculosis (Sambandamurthy et al., 2002). Gene mutation This panCD mutant is from Mycobacterium tuberculosis (Sambandamurthy et al., 2002). A panCD mutant is attenuated in mice (Sambandamurthy et al., 2002). A panCD mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Sambandamurthy et al., 2002). Mycobacterium tuberculosis proC mutant vaccine VO_0002791 Live, attenuated vaccine Research Intravenous injection (i.v.) Intravenous injection (i.v.) Gene mutation This proC mutant is from Mycobacterium tuberculosis (Smith et al., 2001). A proC mutant is attenuated in mice (Smith et al., 2001). A proC mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Smith et al., 2001). Mycobacterium tuberculosis RD1/panCD mutant vaccine VO_0002787 Live, attenuated vaccine Research subcutaneous injection subcutaneous injection Gene mutation This RD1/panCD mutant is from Mycobacterium tuberculosis (Sambandamurthy et al., 2006). Gene mutation This RD1/panCD mutant is from Mycobacterium tuberculosis (Sambandamurthy et al., 2006). Gene mutation This RD1/panCD mutant is from Mycobacterium tuberculosis (Sambandamurthy et al., 2006). An RD1/panCD mutant is attenuated in mice (Sambandamurthy et al., 2006). An RD1/panCD mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Sambandamurthy et al., 2006). At the 10-day time point, substantial increases (relative to naive mice) were detected in IFN-γ positive CD4 and CD8 lung cells taken from the vaccinated mice (Sambandamurthy et al., 2006). Mycobacterium tuberculosis sigE mutant vaccine VO_0002795 Live, attenuated vaccine Research Intratracheal immunization Intratracheal immunization Gene mutation This sigE mutant is from Mycobacterium tuberculosis (Hernandez et al., 2010). A sigE mutant is attenuated in mice (Hernandez et al., 2010). A sigE mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Hernandez et al., 2010). A sigE mutant showed a significantly higher and constant expression of the gene encoding IFN-γ as compared to mice infected with the parental strain. IFN-gamma was significantly upregulated in mice lungs after vaccination with the mutant as compared to infection with the wild type beginning day 1 post-infection and continuing throughout the duration of the experiment (Hernandez et al., 2010). A sigE mutant showed a significantly higher and constant expression of the gene encoding TNF-alpha as compared to mice infected with the parental strain. Vaccination with the mutant significantly up-regulated TNF-alpha in mice lungs on days 1, 21, and 28 post-infection as compared to infection with the wild type (Hernandez et al., 2010). A sigE mutant showed a significantly higher and constant expression of the gene encoding β-defensin 3 in the lungs beginning the 7th day post vaccination until the 28th day as compared to mice infected with the parental strain (Hernandez et al., 2010). The expression of IL-10 was higher in sigE mutant-infected mice lungs as compared to infection with the parental strain, but it was significant only 21 and 28 days after the infection (Hernandez et al., 2010). IL-4 was expressed at a significantly lower level in the sigE mutant-infected mice as compared to mice infected with the wild type pathogen. Significant down regulation of IL-4 by the mutant in mouse lungs was seen days 1-28 post infection as compared to infection with the wild type (Hernandez et al., 2010). The human monocytic cell line THP-1 was obtained from the ATCC, and cultures were grown (Fontán et al., 2008). A sigE mutant upregulates GPR182 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008). A sigE mutant upregulates TBX21 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008). A sigE mutant upregulates APOBEC3B in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008). A sigE mutant upregulates PCDHB1 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008). A sigE mutant upregulates TSC22D4 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008). A sigE mutant upregulates HIST1H2BE in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008). A sigE mutant upregulates STAG3 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008). A sigE mutant upregulates C19orf66 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008). A sigE mutant upregulates CD70 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008). A sigE mutant upregulates HIST2H4A in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008). A sigE mutant upregulates PTCD1 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008). Mycobacterium tuberculosis trpD mutant vaccine VO_0002796 Live, attenuated vaccine Research Intravenous injection (i.v.) Intravenous injection (i.v.) Gene mutation This trpD mutant is from Mycobacterium tuberculosis (Smith et al., 2001). A trpD mutant is attenuated in mice (Smith et al., 2001). A trpD mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Smith et al., 2001). Mycobax Mycobax Sanofi Pasteur, Ltd. VO_0000078 Live, attenuated vaccine Licensed USA Mycobax is a formulation of live attenuated strain of Mycobacterium bovis (Bacillus Calmette Guerin; BCG). PEPf + Advax4 Subunit vaccine Research Intramuscular injection (i.m.) Advax4 adjuvant The PEPf fusion protein was expressed in Escherichia coli, purified by nickel affinity chromatography, and formulated with vaccine adjuvant Advax4 (Junqueira-Kipnis et al., 2022). Intramuscular injection (i.m.) PEPf fusion protein is composed by the Mycobacterium tuberculosis proteases PepA (Rv0125), PepN (Rv2467), and Msh1 (Rv2672) (Junqueira-Kipnis et al., 2022). Fusion Protein This is a fusion protein preparation using PepA from TB (Junqueira-Kipnis et al., 2022). Fusion Protein This is used for fusion protein vaccine development (Junqueira-Kipnis et al., 2022). Fusion Protein This is used for fusion protein (Junqueira-Kipnis et al., 2022). Vaccination with PEPf + Advax4 elicited strong antigen-specific CD4⁺ and CD8⁺ IFN-γ–producing T-cell responses in lymph nodes and lungs, indicating a strong Th1/Tc1 cellular immune response (Junqueira-Kipnis et al., 2022). BALB/c Mice were primed with a single subcutaneous dose of BCG (1 × 10⁶ CFU) and boosted 30 days later with two intramuscular injections of PEPf (20 µg) formulated with Advax4 (1 mg), administered 15 days apart (Junqueira-Kipnis et al., 2022). The BCG prime followed by PEPf + Advax4 boost significantly reduced lung bacterial loads compared to BCG alone (Junqueira-Kipnis et al., 2022). Mice were challenged intravenously with Mycobacterium tuberculosis H37Rv (1 × 10⁶ CFU) 30–45 days after vaccination, and the researchers checked how well the vaccine worked by measuring bacteria levels in the lungs, analyzing immune cells, and examining lung tissue 30 and 45 days later (Junqueira-Kipnis et al., 2022). rBCG-Ag85A/ Ag85B VO_0004757 Recombinant vector vaccine Research Intramuscular injection (i.m.) Three rBCG strains overexpressing immunodominant antigens Ag85B (rBCG::85B), Ag85A (rBCG::85A), or both (rBCG::AB) of Mycobacterium tuberculosis (Wang et al., 2012). Intramuscular injection (i.m.) Recombinant vector construction rBCG strains overexpressing immunodominant antigens Ag85B (rBCG::85B), Ag85A (rBCG::85A), or both (rBCG::AB) of Mycobacterium tuberculosis were constructed (Wang et al., 2012). Recombinant protein preparation rBCG strains overexpressing immunodominant antigens Ag85B (rBCG::85B), Ag85A (rBCG::85A), or both (rBCG::AB) of Mycobacterium tuberculosis were constructed (Wang et al., 2012). Mice in each group were immunized s.c. once at the base of the tail with 1 × 106 CFU of either rBCG::85A, rBCG::85B, or rBCG::AB in a final volume of 100 μL of PBS (Wang et al., 2012). VO_0003057 rBCG::AB strain could provide the strongest short-term and long-term protection in the lung against intravenous infection with virulent M. tuberculosis than rBCG::261 control and other two rBCG strains overexpressing single antigen. The stronger and longer-lasting protection provided by rBCG::AB than rBCG::261 was correlated with systemic in vitro antigen-specific IFN-γ responses (Wang et al., 2012). mice were challenged with virulent M. tuberculosis strain 6 weeks after immunization (Wang et al., 2012). rBCG-Ag85A[Tokyo] VO_0000565 Live, attenuated vaccine BCG and Mycobacterium antigen Ag85A (Sugawara et al., 2007). Recombinant vector construction The Ag85A gene was amplified by PCR and subcloned into the pCR4 vector. The gene was inserted into the pBBN vector (Ag85A-HA) possessing a hemagglutinin (HA) tag at its 5′ end. The Ag85A-HA was expressed in Escherichia coli. The Ag85A-HA gene was then introduced into the downstream region of the pHPS integration vector. The vector was then electroporated into BCG Tokyo. The resulting recombinant was named (rBCG-Ag85A[Tokyo]) (Sugawara et al., 2007). Hartley female guinea pigs Hartley female guinea pigs (group 2) were vaccinated with BCG Tokyo (Sugawara et al., 2007). rBCG-Ag85B/A (Mycobacterium tuberculosis) Recombinant vector vaccine Licensed Intramuscular injection (i.m.) Intramuscular injection (i.m.) rBCG-LTAK63 Live, attenuated vaccine Research Intramuscular injection (i.m.) The purpose of rBCG-LTAK63 is to create an improved tuberculosis vaccine by enhancing the immune response that standard BCG produces. The recombinant BCG strain expresses the detoxified LTAK63 A-subunit, which acts as a built-in adjuvant to strengthen early and long term protective immunity against mtb. Its goal is to generate faster IFN-γ responses, better control of bacteria growth, and reduced lung damage compared to traditional BCG. LTAK63 is a detoxified form of the E.coli heat labile toxin that keeps strong immune boosting activity but removes toxicity. (Marques-Neto et al., 2025) Intramuscular injection (i.m.) At 7 days post immunization, rBCG-LTAK63 triggers a strong early innate activation: stress genes, autophagy, cAMP, and early IFN-γ pathways, much higher than normal BCG. By 90 dpi, this develops into durable immunity, with sustained IFN-γ gene activity and stronger Th1/Th17 memory response, showing enhanced long term protection before infection. BALB/c mice They used female 6 weeks old BALB/c mice, which were immunized once with wither rBCG-LTAK63 or would type BCG at a dose of 1*10^8 CFU delivered subcutaneously into the back. Saline treated mice served as controls. After vaccination, immune responses and gene expression were evaluated at 7 and 90 days post immunization. (Marques-Neto et al., 2025) rBCG-LTAK63 reducing lung bacterial load by about 1 log at 7 dpc and nearly 2 logs by 30 dpc, while also lowering inflammation and preserving healthy lung tissue.(Marques-Neto et al., 2025) All the mice were challenged intranasally with 500 CFU of M. tuberculosis H37Rv at 90 days.(Marques-Neto et al., 2025) recombinant S. typhimurium secreting M. tuberculosis ESAT-6 VO_0000535 Live, attenuated vaccine ESAT-6 has been tested as a skin-test reagent in cattle and guinea pigs. The subunit vaccine which combines ESAT-6 with monophosphoryl lipid A (MPL) and dimethyl dioctadecylammonium bromide (DDA) was developed and tested for use in BALB/c female mice (Mollenkopf et al., 2001). Monophosphoryl lipid A (MPL) and dimethyl dioctadecylammonium bromide (DDA) (Mollenkopf et al., 2001). This vaccine utilizes an attenuated Salmonella typhimurium that secretes a T-cell antigen called ESAT-6 of Mycobacterium tuberculosis through the hemolysin secretion system of E. coli (Mollenkopf et al., 2001). ESAT-6 of Mycobacterium tuberculosis (Mollenkopf et al., 2001). ESAT-6 can induce IFN-gamma production and activate T cells in human peripheral blood mononuclear cells in TB patients. Fusion protein with adjuvant BALB/c, female Recombinant S. typhimurium was injected into tail vein of each mouse and cured with10 mg ampicillin injected intraperitoneally (i.p.). Five mice used per group. Group A received high-dose vaccine 90 days pre-challenge and was cured twice with ampicillin. Group B received low-dose recombinant vaccine 60 days pre-challenge. Group C was vaccinated at 90, 60, and 30 days pre-challenge. Group D received s.i. prime with bacteria and naked DNA 90 days pre-challenge, two DNA boosts at 60 and 30 days pre-challenge, and curing twice. Group E received the same vaccinations and challenges as in D, though in a different order. GeneGun and pcDNAesat or PIRESesat were used during vaccination (Mollenkopf et al., 2001). A significant difference in colony-forming units was seen at 10 versus 60 days post challenge (Mollenkopf et al., 2001). S. typhimurium induced a small but signifcant degree of protection in lungs, though only small degree of protection in the liver at day 60 (Mollenkopf et al., 2001). Spleens were enlarged after procedure, though CFU analysis revealed that the mice were free of Salmonella bacteria (Mollenkopf et al., 2001). Challenge route was same as in vaccination (tail vein injection), though employed 5x10^5 CFUs of the virulent M. tuberculosis H37Rv i.v. (Mollenkopf et al., 2001) RPC4/DIMQ Subunit vaccine Research subcutaneous injection Adjuvant DIMQ compose DDA (dimethyltrihexadecylammonium) and Imiquimod (IMQ).(Wang et al., 2025) The RPC4 fusion protein was recombinantly expressed in Escherichia coli, purified by Ni-NTA affinity chromatography with endotoxin removal, and emulsified with the liposomal adjuvant DIMQ (DDA combined with imiquimod).(Wang et al., 2025) subcutaneous injection RPC4 fusion protein, consisting of Rv2131c, Rv1984c, Rv2029c and Rv0388c. Fusion Protein This is use in fusion protein preparation(Wang et al., 2025). Fusion Protein Use in fusion protein reparation(Wang et al., 2025). Fusion Protein This is use in fusion protein preparation(Wang et al., 2025). Vaccination with RPC4/DIMQ, particularly as a BCG prime boost regimen, induced strong Th1-biased immune responses characterized by elevated IFN-γ, TNF-α, and IL-2 secretion, increased multifunctional CD4⁺ and CD8⁺ T cells, and high antigen-specific IgG2a antibody titers.(Wang et al., 2025) C57BL/6 Mice were primed with BCG at week 0 and boosted subcutaneously with the RPC4/DIMQ subunit vaccine every 3 weeks for a total of three booster immunizations, with immune responses assessed at week 9.(Wang et al., 2025) SHR3/DMT Subunit vaccine Research subcutaneous injection DMT adjuvant containing DDA, MPLA and TDB. Recombinant fusion protein expressed in E. coli, purified by Ni-NTA affinity chromatography, dialyzed into phosphate-buffered saline, and formulated with DMT adjuvant as a subunit vaccine(Zhang et al., 2024). subcutaneous injection SHR3 fusion protein, composed of Rv1626, Rv2866 and Rv1884(Zhang et al., 2024). Fusion Protein use in preparation of fusion protein(Zhang et al., 2024). Fusion Protein This is use for preparation of fusion protein(Zhang et al., 2024). Fusion Protein Use in preparation of fusion protein(Zhang et al., 2024). Vaccination induced strong antigen-specific Th1-type immune responses characterized by increased IFN-γ, TNF-α, and IL-2 secretion from CD4⁺ and CD8⁺ T cells, with low IL-4 levels. The BCG+SHR3/DMT group produced the highest IgG titers and IgG2a/IgG1 ratios (>1), indicating a Th1-dominant response (Zhang et al., 2024). C57BL/6 Mice were immunized subcutaneously. The BCG+SHR3/DMT group received BCG priming at week 0 followed by two booster doses of SHR3/DMT at 3-week intervals. SHR3/DMT and subunit groups were immunized every 3 weeks over a 9-week period(Zhang et al., 2024). Spore-FP1 Subunit vaccine Research subcutaneous injection The adjuvant is PolyI:C (White et al., 2023). The FP1 fusion protein is adsorbed onto heat-inactivated Bacillus subtilis spores and formulated with PolyI:C in sterile saline (White et al., 2023). subcutaneous injection The antigen is a fusion protein composed of Ag85B, Acr (HspX) and HBHA heparin-binding domain (short 6 kDa N-terminal domain) (White et al., 2023). Fusion Protein This is use in fusion protein preparation (White et al., 2023). Fusion Protein This is use in fusion protein preparation (White et al., 2023). Fusion Protein This is use in fusion protein preparation (White et al., 2023). Vaccinated guinea pigs showed enhanced antigen-specific immune responses associated with reduced granuloma formation, decreased lung pathology, and lower bacterial loads compared with controls (White et al., 2023). Guinea pigs were first primed subcutaneously with either Spore-FP1 or BCG. For the booster regimen, Spore-FP1 was administered intranasally at later time points following the prime immunization. In the BCG prime–boost group, animals received a single subcutaneous BCG vaccination followed by intranasal boosts with Spore-FP1 before aerosol challenge (White et al., 2023). Spore-FP1 significantly reduced lung bacterial burden and tissue pathology compared with controls and improved protection when used as a BCG booster (White et al., 2023). Four weeks after the final immunization, guinea pigs were challenged with low-dose aerosol Mycobacterium tuberculosis H37Rv, and bacterial burden in lungs and spleen was assessed (White et al., 2023). 6-phosphofructokinase PfkB Mycobacterium tuberculosis H37Rv 887491 15609166 Rv2029c AL123456 NP_216545 83332 2275404 2276423 - 6-phosphofructokinase PfkB 5.66 32325.93 339 phosphohexokinase (phosphofructokinase) >NC_000962.3:2275404-2276423 Mycobacterium tuberculosis H37Rv, complete genome TTCATGGCGAGGCTTCCGGGTTAACGATCGGGTGCCAAACGTATTGATCCTGCCCGACTTCGGTGGGTTC GGCCGCCAGCTCGAAGAACCTCTCCACATCGTCGCGATTGCAGGCCGCGGTGCCTGGCGTCAGCAGCATG GCTGCACCTGCCGCGTTTCCCAAGCGAACGGACTTGATGAGCGACCAGCCACGGCTGAGGCCCACGGTAA TCGCGGCCACCATCGCGTCGCCGGCGCCGACACCGCTAACCGCGGTCATCGGAATCGACGAAAATCGATG GCTCGCATGTCGTGTGGCCAATAGCGCGCCCTGAGATCCAAGCGAGACCACCACGACCTCGGCGCGCCCA CGGTCAATGAGTTCGTGTGCGGCGGCCAGTTGTTCGGGCTCGGTCAGCAGTTCGGATCCGACGCACTCGC GCAGTTCCCGCACGCTCGCCTTGAGAAGAAACACCCCGGACGAAATGTGCTGCAACCCGCCACCAGATGT ATCCAGGATCAGCGGAGTGCTCGATCGGCGGCAGATGTCGGCAACCCGCTGATAGTAGTCGGCAGCCACA CCTGGCGGCAGGCTGCCACTGGCCACCACAAAGGCGGCCGAAGCCGCCGCACCGCGCAGTTCGTCGAGGC ATTGCTCCTGCTCCGCGACGGTCAGCGACGGCCCCGGAAGCACGAAACGATACTGCTTGGCGGTCCTGGA CTCGTTGACCGTGAAGCTCTCCCGCGTCGAGGCCGCGATCGGAATGACGCGAAATGGCACTCCCGCATCA CCGAGCAGCGCCATCAGCAGGCTCCCGGTCGACCCGCCGGCCGGGAACAGTGCTGTCGAGCAACCGCCGA GGACATGCACAATGCGGGCGACATTGATACCGCCGCCGCCGGGATCGTAGCGAGGTGCGCCACAACGCAT TTTCTCGGTCGGGCGCACCACGTCGACGCTCGTCGTGATGTCCAAGGCGGGGTTCATGGTCAAAGTGATG ATTCGCGGCTTGCCTTCGTCCCACGCCGCTGGCTCCGTCA >NP_216545.1 6-phosphofructokinase PfkB [Mycobacterium tuberculosis H37Rv] MTEPAAWDEGKPRIITLTMNPALDITTSVDVVRPTEKMRCGAPRYDPGGGGINVARIVHVLGGCSTALFP AGGSTGSLLMALLGDAGVPFRVIPIAASTRESFTVNESRTAKQYRFVLPGPSLTVAEQEQCLDELRGAAA SAAFVVASGSLPPGVAADYYQRVADICRRSSTPLILDTSGGGLQHISSGVFLLKASVRELRECVGSELLT EPEQLAAAHELIDRGRAEVVVVSLGSQGALLATRHASHRFSSIPMTAVSGVGAGDAMVAAITVGLSRGWS LIKSVRLGNAAGAAMLLTPGTAACNRDDVERFFELAAEPTEVGQDQYVWHPIVNPEASP Protective antigen Ag85B from M. tuberculosis H37Rv Mycobacterium tuberculosis H37Rv VO_0012364 885785 15609023 Rv1886c AL123456 NP_216402 EMBL: AE000516 EMBL: AF198032 EMBL: BX842578 EMBL: U38939 EMBL: X62398 GenomeReviews: AE000516_GR GenomeReviews: AL123456_GR InterPro: IPR000801 KEGG: mtc:MT1934 KEGG: mtu:Rv1886c PDB: 1F0N PDB: 1F0P Pfam: PF00756 PIR: C70516 TIGR: MT1934 TubercuList: Rv1886c UniProt: P31952 83332 2134889 2135866 - diacylglycerol acyltransferase/mycolyltransferase Ag85B 5.72 33091.46 325 Rv1886c, (MT1934, MTCY180.32), len: 325 aa. fbpB (alternate gene names: mpt59, 85B), precursor of the 85-B antigen (fibronectin-binding protein B) (mycolyl transferase 85B) (EC 2.3.1.-) (see citations below), highly similar to other Mycobacterial antigen precursors e.g. P12942|A85B_MYCBO ANTIGEN 85-B PRECURSOR from Mycobacterium bovis (323 aa); P21160|A85B_MYCKA ANTIGEN 85-B PRECURSOR from Mycobacterium kansasii (325 aa); etc. Also highly similar to Mycobacterium tuberculosis antigen precursors: Rv3804c|fbpA (338 aa), Rv0129c|fbpC2 (340 aa), and Rv3803c|fbpC1 (299 aa). TBparse score is 0.912.; mpt59; 85B FUNCTION: Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity (UniProt: P31952) SUBCELLULAR LOCATION: Secreted protein (UniProt: P31952) SIMILARITY: Belongs to the mycobacterial A85 antigen family (UniProt: P31952) >NC_000962.3:2134889-2135866 Mycobacterium tuberculosis H37Rv, complete genome GTCAGCCGGCGCCTAACGAACTCTGCAGGTCACCCTTCATGGCGTTGAGCTGAGCGCCCCAGTACTCCCA GCTGTGCGTGCCGTTGGGCGGGAAGTTGAACACGGCGTTGTGCCCGCCCGCGGCGTTGTACGCATCCTGG AACTTCAGGTTGCTGCTACGAACGAAGTTCTCCAAGAACTCGGCGGGTATGTTGGCACCGCCCAACTCGT TCGGGGTGCCGTTCCCGCAATAAACCCATAGCCGGGTGTTGTTTGCGACCAGCTTGGGGATCTGCTGCGT AGGGTCGTTGCGCTCCCATGCCGGGTCACTCGAGGGACCCCACATGTCTGCGGCCTTGTAACCGCCGGCG TCACCCATCGCGAGGCCGATCAGGCTAGGCCCCATCCCCTGAGAGGGGTCCAGCAGGGCCGACAGCGAGC CGGCGTAGATGAACTGCTGGGGGTGGTAGGCGGCCAAGATCATTGCCGACGAGCCGGCCATCGACAAGCC GATTGCAGCGCTGCCGGTGGGCTTCACGGCCCTGTTGGCGGACAACCATTGCGGCAGCTCGCTGGTCAGG AAGGTTTCCCACTTGTAAGTCTGGCAGCCAGCCTTACCGCAGGCCGGGCTGTACCAGTCGCTGTAGAAGC TGGACTGCCCGCCGACCGGCATGACTATCGACAGTCCCGACTGGTAGTACCACTCGAACGCCGGGGTGTT GATATCCCAGCCGTTGTAGTCGTCTTGGGCGCGCAGGCCGTCGAGCAGATAAACCGCAGGTGAGTTGTTC CCACCGCTCTGGAACTGAACCTTGATGTCGCGGCCCATCGACGGCGACGGCACCTGCAGGTACTCGACCG GCAGCCCCGGCCGGGAGAACGCGCCCGCGGTTGCCGCTCCGCCGGCAAGCCCCACCAGGCCCGGAAGGAC TACAGCCGCTGCCGTGCCGATCATCAATCGGCGTCCCCAAGCTCGAATCTTTCGGCTCACGTCTGTCA >NP_216402.1 diacylglycerol acyltransferase/mycolyltransferase Ag85B [Mycobacterium tuberculosis H37Rv] MTDVSRKIRAWGRRLMIGTAAAVVLPGLVGLAGGAATAGAFSRPGLPVEYLQVPSPSMGRDIKVQFQSGG NNSPAVYLLDGLRAQDDYNGWDINTPAFEWYYQSGLSIVMPVGGQSSFYSDWYSPACGKAGCQTYKWETF LTSELPQWLSANRAVKPTGSAAIGLSMAGSSAMILAAYHPQQFIYAGSLSALLDPSQGMGPSLIGLAMGD AGGYKAADMWGPSSDPAWERNDPTQQIPKLVANNTRLWVYCGNGTPNELGGANIPAEFLENFVRSSNLKF QDAYNAAGGHNAVFNFPPNGTHSWEYWGAQLNAMKGDLQSSLGAG Protective antigen In mice, a primary vaccination with Ag85B-encoding plasmid DNA (DNA-85B) was protective against Mycobacterium tuberculosis (MTB) infection and associated with Ag85B-specific CD4+ T cells producing IFN-gamma and controlling intramacrophagic MTB growth [Ref1404:Palma et al., 2007]. Apa Mycobacterium tuberculosis H37Rv VO_0011175 885896 57116926 Rv1860 AL123456 YP_177849 83332 2107735 2108712 + hypothetical protein 4.7 30636.65 325 Rv1860, (MT1908, MTCY359.0013), len: 325 aa. apa (alternate gene names: mpt32, modD), Ala-, Pro-rich 45/47 kDa secreted protein, very similar to P46842|N43L_MYCLE from Mycobacterium leprae (287 aa), FASTA scores: opt: 1166, E(): 0, (66.4% identity in 298 aa overlap). Known to be glycosylated fibronectin-binding protein (see some citations). CHANGES IN THE MANNOSYLATION PATTERN OF THIS PROTEIN AFFECT ITS ABILITY TO STIMULATE T-LYMPHOCYTE RESPONSE. MAJOR IMMUNODOMINANT ANTIGEN THAT HAS POTENTIAL AS A VACCINE AGAINST TUBERCULOSIS. APA-ELISA COULD BE USED IN DIAGNOSIS. TBparse score is 0.924.; mpt32; modD >NC_000962.3:2107735-2108712 Mycobacterium tuberculosis H37Rv, complete genome CATGCATCAGGTGGACCCCAACTTGACACGTCGCAAGGGACGATTGGCGGCACTGGCTATCGCGGCGATG GCCAGCGCCAGCCTGGTGACCGTTGCGGTGCCCGCGACCGCCAACGCCGATCCGGAGCCAGCGCCCCCGG TACCCACAACGGCCGCCTCGCCGCCGTCGACCGCTGCAGCGCCACCCGCACCGGCGACACCTGTTGCCCC CCCACCACCGGCCGCCGCCAACACGCCGAATGCCCAGCCGGGCGATCCCAACGCAGCACCTCCGCCGGCC GACCCGAACGCACCGCCGCCACCTGTCATTGCCCCAAACGCACCCCAACCTGTCCGGATCGACAACCCGG TTGGAGGATTCAGCTTCGCGCTGCCTGCTGGCTGGGTGGAGTCTGACGCCGCCCACTTCGACTACGGTTC AGCACTCCTCAGCAAAACCACCGGGGACCCGCCATTTCCCGGACAGCCGCCGCCGGTGGCCAATGACACC CGTATCGTGCTCGGCCGGCTAGACCAAAAGCTTTACGCCAGCGCCGAAGCCACCGACTCCAAGGCCGCGG CCCGGTTGGGCTCGGACATGGGTGAGTTCTATATGCCCTACCCGGGCACCCGGATCAACCAGGAAACCGT CTCGCTCGACGCCAACGGGGTGTCTGGAAGCGCGTCGTATTACGAAGTCAAGTTCAGCGATCCGAGTAAG CCGAACGGCCAGATCTGGACGGGCGTAATCGGCTCGCCCGCGGCGAACGCACCGGACGCCGGGCCCCCTC AGCGCTGGTTTGTGGTATGGCTCGGGACCGCCAACAACCCGGTGGACAAGGGCGCGGCCAAGGCGCTGGC CGAATCGATCCGGCCTTTGGTCGCCCCGCCGCCGGCGCCGGCACCGGCTCCTGCAGAGCCCGCTCCGGCG CCGGCGCCGGCCGGGGAAGTCGCTCCTACCCCGACGACACCGACACCGCAGCGGACCTTACCGGCCTG >YP_177849.1 hypothetical protein Rv1860 [Mycobacterium tuberculosis H37Rv] MHQVDPNLTRRKGRLAALAIAAMASASLVTVAVPATANADPEPAPPVPTTAASPPSTAAAPPAPATPVAP PPPAAANTPNAQPGDPNAAPPPADPNAPPPPVIAPNAPQPVRIDNPVGGFSFALPAGWVESDAAHFDYGS ALLSKTTGDPPFPGQPPPVANDTRIVLGRLDQKLYASAEATDSKAAARLGSDMGEFYMPYPGTRINQETV SLDANGVSGSASYYEVKFSDPSKPNGQIWTGVIGSPAANAPDAGPPQRWFVVWLGTANNPVDKGAAKALA ESIRPLVAPPPAPAPAPAEPAPAPAPAGEVAPTPTTPTPQRTLPA Protective antigen We show that the 50- to 55-kDa Apa protein, specified by the Rv1860 gene of M. tuberculosis, can elicit both lymphoproliferative response and gamma interferon (IFN-gamma) production. A poxvirus recombinant expressing the Apa protein conferred a significant level of protective immunity in guinea pigs against a challenge dose of virulent M. tuberculosis [Ref1186:Kumar et al., 2003]. APOBEC3B Homo sapiens 9582 22907025 CTA-150C2.15-003 AL022318 NP_004891 9606 22 22346831 22357253 apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B 5.94 44235.85 382 Also known as ARP4; ARCD3; PHRBNL; APOBEC1L; bK150C2.2; DJ742C19.2; FLJ21201 >gi|157729478:22346831-22357253 Homo sapiens chromosome 22, alternate assembly HuRef, whole genome shotgun sequence CACAGAGCTTCAAAAAAAGAGCGGGACAGGGACAAGCGTATCTAAGAGGCTGAACATGAATCCACAGATC AGGTACCGCTGCCCACTCTGCCTGCTGGGCCCCTCCTGCCCCTTCCTGCCTGGTGGTCCTGCTGGGCCTC AACCCTGGCCTCCCCCCGCCCCTGCCCCAGCCCTGGGCTCCCTCCCCTCTGGCTCCCCTGCCACACGAAT CCCAGTCAGGCTCTTTGTCCTGCTGTGTGGTCACCCCACTGCTGCTTCTGAATGGGCTGCCTCCCCTACC TGCACCAGCCCAGGCCCCGTGCTGAGACTCTCCCTTAAAGTCATGCCCGGACTGGTGCTCCCCGCCCTGG GAGGGTGCTCCCTCTGTGTGCTTCCTGCCACTCCTGAGCTCAGAAACTGGGAGAATTGAACCAAAGGGTA ATTGGAGCAATCAGGCATTTTTGTTCTCAGTGTTTTGATGAAATTTCTGTAAGAATTACTTTGCTTAAAT ACAAAGTCTTAGGAGAGGGTGCGGGGGTAGGAATGGTCTCTGAAGCACATGATAAATCGTGTCATCCAAG GATGACTCCATGGTGAGCAGATGTCCCCAGACAGGTTCCACTCCAAGGTCACTCTTCATCTTTTAGAACA TGACCAGTGGTTGAGCGTGGTGGCTCACACCTGTAATCCCAACACTTTGGGAGGCCAAGGCGGGCGGATC ATGAGGTCGGGAGTTTGAGACCAACCTGGCCCATATGGTGAAACCCCGTCTCTACTAAAAAACATACAAA AATTAGCCAGGCATGGTGGCGCATCTCTAGTCCCAGATACTCAGGAGGTTTAGGCAGGAGAATTGCTTGA ACCTGGGAGGTGGAGGTTGCAGTGAGCTGAGATCATGCCACTGCACTCCAGCCTGGGCGACAGAGTGAGA CTTCATCAAAAAAAAAAAGAAAGAACATGATCAGTAACATGGAATGTGAGAATGTGGGAAAAAAGTGCAA TGATGAGGAGAAATGCTCTGATTTTAAAATTTAGTGAAAAGAGAGCCCAGTCCCCAACAGCTTTCTCCTA CAGGATCCGTGATGCAGAGGCTGGACAGGGCTGGCCTGGAAAGGGGCCCCTGCTCCATCCGTCCCCAGCT CTGTGGCCTCGGCAGGTTACCCCGCCTCTCTGTGCCTCTGGCATCTCCTCTGTAAGATGGGAATGGCCCC TGCAGGCCTAGGGTAGCCTCACGTGAGCTCACACCCGCTCAGCACTTAGAAGAGTGGCCTGGGCCTCAGA ATTCAGTTCTACCATGATTTTAAAATAATGTTAACACGCAGAGTGAAACATCAGGAAGGAATTGAGCTGA AAGGTTAAAAGCTATTTTTTAGGGGTGAAGGGTTTTATTCTCTTTTGCATTCTCCTAATGGGTTGGGGAG TGGGTTGGCAGAAATACTAGGAGGGTGGGGAATGTACCCCTGGAAAGGCCAGAGTTGGACCCGAGCTGGG AGAGATCACCCCAGCCCGCCTGCCAGCATCCCCTCCTCTTCCTCTCCCCTCAGTCTTCCTGCCTGGGAAG GCAGCAGAAATTCTCAGGGCTGTGGAGGGGTCGGGGAGGCCCAGGGCCATCCTGGGAGCTGTGTTCAGTG GACATGAGCCCCGAGGACTCCCGGGAGGGCTCCCTGCATGGGCCGGTTTCTCTCTTGTGCCTTCAGAAAT CCGATGGAGCGGATGTATCGAGACACATTCTACGACAACTTTGAAAACGAACCCATCCTCTATGGTCGGA GCTACACTTGGCTGTGCTATGAAGTGAAAATAAAGAGGGGCCGCTCAAATCTCCTTTGGGACACAGGGGT CTTTCGAGGCCAGGTACCACCCAAACTTCAATCGAATCACAGGCAGTGTTGCAGGAATTAGAGGACTGGA GAGACTGATATGGGTGAGACAGGAGGATTTATTTAGGTGCACTGGTTCAGCAGACTCACATCCAAAAAGG CTGAGCCCTTAACAAAGACAGACTGAGGTATTTATAAGCAAACTTACAGAAGCAGAACAAAGGCGATTAA TCAAACAGTGACAGGTCACATAATCTATAGCATAACAGATGACTTAGCATAACTTGGGGCCTTCTATAGC TGGTGGCCTTGCAGCTACATCAAAAGGAAAACAGGAACTGGCTAAATACAGACATTTGTAAAATATAGTT ATAATGGTTCTGGAAAGGAGAGACAGTAAAGGAATTTGTCTTTTTTTTAAAAAATTTTTTTAAAATTTTC TTCAACCTTGCCCTGGAGGGGTGTGGGGGTGGTGTCTGGAGCCCATTCCTTTGGCCTTGACTTTTCAGGC AGTGTTATAACTGTCGTTGGAGTGAGCTGGCTAGGCAGAGGAAAACTTGTTCTTCTTTTCTTTTTAACCC TTGCTACAGTAGGACCTAAGCAGCTGGAAATGCAGAAAACACAACAATAAGTAAAATGCCCAGCACTGGG CTCTCAATTGTGTATTTTTCCCACATTTCTTTTTTCTCTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTCC GAGATGGAGTCTCGCTCTGGCACCCAGGTTGGAGTGCAATTGCACAATCTTGGCTCACTACAACCTCCAC CTCTCAGGTTCAAGTGATTCTTCGGCCTTAGCCTCCCGAATAGCTGGGATTACAGAAGGCTGCCACCACG CCCAGTTAATTTTTTCTATTTTTAGTTGAGACTGGGTTTCACCATGTTGGCCAGGCTGGGGAATTCCTGA CCTCAGGTGAGTCACCTGCCTTGGCCTCCCAAAGTGCTGGGGTTACAAGCATGAGTCACCGCACCTGGCC ACTTCCCACAGTTCTGTTTTTTTGTTTTGTTTTGTTTTGGTTTTTTTTTAAGACTGAGTTTCACTCTTTG TTGCCTGGGCTGGAGTGCAATGACTCACTCTCAACTTCCACCTGCCAGGTTCAAGTGATTCTCCTGCCTC AGCCTGTGAGCTGAACAGTCACATGAGGGTGAAGGTTGTTGCCACAAGCTCAGGGGATGCTCCAGACAGA GTGGCCTGGGATGTCGAGTCACAGCTGCTCCATGCCACGGGGACAAGAGGAAGCAGGAGTTTAGTCTGAC ACACTGCCCTTCCAGATAGAGGGCAAGAGACAGAAAGAGGGGCTGAAGTCACCCTTGAATAACCACAGCA GTGGCACCCACAAAGGTGCAGTCCCCACAGCCTCATAGCCTCTTCTAGGTGCCACCTCTTAAGGCCATTA CAATAGCCATTAACACTGAACGTGAGCTTTGGAGCAGACAATCACTCAAACTAAACAGGGGGGATGGAGG AAAGGAGCTTCAATGGCAAGATCCCCTGGGCTCCTGTCCTGGCCCCTCCTCTCCCTGCCCCACCCCTGCA CTCCTCCTGCTCCCCCTCTCAGAGCATCCCCTGCCCCCTGCTCCTCTCCCAGGTGTATTTCGAGCCTCAG TACCACGCAGAAATGTGCTTCCTCTCTTGGTTCTGTGGCAACCAGCTGCCTGCTTACAAGTGTTTCCAGA TCACCTGGTTTGTATCCTGGACCCCCTGCCCGGACTGTGTGGCGAAGCTGGCCGAATTCCTGTCTGAGCA CCCCAATGTCACCCTGACCATCTCCGCCGCCCGCCTCTACTACTACTGGGAAAGAGATTACCGAAGGGCG CTCTGCAGGCTGAGTCAGGCAGGAGCCCGCGTGAAGATCATGGACTATGAAGGTGAGAGGTGGAGGGGTC AGGGGAGCGTGAGCGGGAGGAACAGCATGAAAGATGGATGGATCTGCAATGCCATGGCTGGGGGTGTCCC AGGGCAGCCTGCAGGGGTGGGACTGGCACTGACTGCAACTGACAGCCAGGAGACCAGGCCTGGGAGGACA GGCCAGGGTCAGGGGAGAGCCTGACTGCTTCCCGCTTCTTCATCTCAGAATTTGCATACTGCTGGGAAAA CTTTGTGTACAATGAAGGTCAGCAATTCATGCCTTGGTACAAATTCGATGAAAATTATGCATTCCTGCAC CGCACGCTAAAGGAGATTCTCAGGTGAGGGTCTCCCTCTGGCCTCATCATCTCTCTCCTCTCACCTGCTC ATCCTCCTGAGGCCTCCGTTGGCCTGGCCCTCCCGCCCTCCCTCTTGACCCACTGCCTGCCCTCATGGTC ACACCACCTTCCCTTAACTCCTGGTGCTCCCTCCACACTGCCTCCTCCCTGCTTTCCTGGGCCCTTCCTG TGAGTAAGAGGCCCCTTCTGCCTCTAAGGCACCTGGGTCTTGGACTAGAGGAACAAACAGCAAGACAGGG TGGCCTGGGACACCAGCCGCTGCCCTTCTGTGACATGGGGACAAGAGGAGGCAAGAGCTCAGCCTTCCAG GGAACAACTCTGGGCAGGAGATGTGGAAGGAAGGAGCTCAGTGTGGGGGCACCCATGGCATCCTGGGGGG ACATCTGAGGGCCACCCCCACCCGCTATTCCTCCCTCCAATGGTGGCCTCTGAGTGTGAAGGCATGGGGG AAGGCAGACACCTGCCCCTGACTCTCCCTCACTACCACATAGCTGCTGGGTGGCTGGGGACGGGGGGGGT CCCTGGGATGATTCCTGAGGGCAGGATCCAGGGGTCCCTCCAGATGTCCTCCCATCCAGGTGAGAAAGCA GCATAAAGTGAGGCTTGCACTCAGATGGGCCTGGGAGGTCACTCACAGCGCAGGTGTCTCCTGGAACAAG GGCCCTGGAAGATAGAAATAGAATCCAAACCCAGGACTAACATCAGGGCCAAGACAAGCCTGCCAGGGAG GCTGCACATGAAGCCCCAGATCAGGGACCACTGCCCGCTCTGCCCATGGGGCCTCTGCTGCCCTTTCCTG CCTGGTGGTCCTGCTGGGCCTCAGCCCTGGCCTCCCCCTGTCCCAGCCCCAGCCCTGGGCTCTCTCCCCT CCTGTTCCTCTGCCACCCCCACTCCCAGCCAGGCTCTTTGCCCTGTTGTGTGGTCGCCCCACTGCTGCTT CTGAATGGGCCACCTCCCCCACCTGCCCCAGGCCAGACCCCCTGCTGAGACTCTTCCCTGAAAGTCACCG CTGGGCTGGTGCTCCCTGCCCTGGGAGGGTGCTCCCTGTATATGCTTCCCACCATTCCTGAGCTCTGGAA CTGGGAGAATTGAACCAAAGGATGATTGGAGCAATCAGGCATTTTGTTCTTAGCATTTTGATGGAATTTC TGTAAGATTTACTTTGTTTCAACACAAAGTCTTAGGAGAGGGTGTGGGGGAGGAAATGGTCTCTGTACCA GAAAATAAGTCATTTCTTCCAAAGACGCCTCCGCTGTGAGCAGAGGAGGATGCACACGACCCCAGACACA GGCTCCTCCTCCGTGAGCACCATTCACCTTTTAGAATGACACCTGCAACATGGAGTGTAGGAAAACATAT GATCAGGAGAAATGCTCTTATTTTAGGCAGGGCACGGTGGCTTATGCCTATAATCCTAGCTCTTTGGGAG GCTGAGGTGGGTGGATCACTTGAGGTCAGGAGTTGGAGATCTGCCTGGCCAGCGTGGCAAAACCTCCTCT CTACTGAAAATACAAAAAATGAGCCAGGCATGGTAGCGGGAGCCGGTAATCCCAGCTACTTGGGAGGCTG AAGCAGGAGAATCGCTTGAACCCGGGAGGGACAGCAGTGCTGGTCACGGAAACACCCAGGACTCAGGACT GAAGCACAAGTGTTTCCAGTCCCCACACACTAAAAAGGAGACAAGAACTCCTCTTCGAGCTTTTCTGATG AGCACTCACCTCTCACCCTCAAACCCCTGGGGCCTCTCTTTTCCTCCTGACCCTCTCTCTGGACCTGGCC TCTTGCTCTAGGTTCCCAGTTTTGGTCCCAGTGCTGGTCTCTCCTCCAATGGTACCAATTCCAGGTCTCC TTCCACCTCCTGGGGCAGATGCACAGGGAGCCTGAAAATCCCAGTGGATAATGATGCTGTGCCCCAGCCA GTGAAGACAGTTGGAGAGGGGATTCTCTTCACTTGTCTTTAAATGAGCATCTACTATTTCTTGCAAATGT TCCATGTCTCTGGACTTGGCCTAAGCCATTTTTATGTTCAGTGTCATCTCGTTCTCCCTAATATTTAGAA AAGGGTGTTGCTTGTAGGACCACTTTGCTATGAAGAAGCCTTGTTTCCTTTTCTTGAGAACACAAAGCAA GTGAGTGGTAGAGCCCAGATTCATACCCAGGTCTACTAGAAATTGTGGCAAACAGAGATTCCTTCCTTCC TTCCTTCCTTCCTCCCTTCCTTCCTTCCTTCCTTCCTTGCTTCCTCTCTCTTTCTCTCTTTCTCTCTTTC TCTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTT TCTTTCTTTCTTCTCTCTCGTCTTTCTTCTTTTGCTGCTGCCTCCAAACAGAGATTTTTCTAATTCTGGT TTTGTCAGGATGTCTACAGAGGGGCCGGGAGAGGCCTTCAAGGTGGGGCTGGTGTTTCCAGCCCAGGAGT CCTGAGCTGCCCCATCTGGCCTGAGAAGCACGTTTGGGAGACCTCATGGCATCCAAAGGGACGTGGAAAA TGCTATGAATTATCCAAAAAGATGTCCCTGGCAGGCTCCTCTCCCCAATGATCCCCCTCAGGAGTGTCCC TGCCCCAATGCTGGGTGTGGTAGGAGTTAACCCTGCAGGCAGGAGGAAGCCCCAGCAGGTCCATCTCCAG CAGGGGCTGTGGGTGAGCAGAGCCAGGACAGGGGTGCATGGTGAGGCCAGGGAAGGAGACCCAGCTCTAC CCCAGGGAGAGGGGCAGGGTCCTCCCCGGAGGGCCTGAGCACACTGAGCTGACCCTGGGGAGACCCTGAC AAGGCCTAAACAGGCCCCGGGGCTGTGTTGACTTCCTGATAATCCACCAGAAGGGGTCAGAGAGAGAGGA TCAGTGGCCCCCACAAAGGGAGTGGAAGCGCCTCCTCTGACAGGTGCCTCAGTATATGGGGAGCAGGGAA GGAGTGGGGGGTGCAGGAGAGGAGCGAGAGGTAGTCCCAGGAGGAGTCTTAGGGCTTTTGGTTTCCCCTG TCTTTGTCCACAGATACCTGATGGATCCAGACACATTCACTTTCAACTTTAATAATGACCCTTTGGTCCT TCGACGGCGCCAGACCTACTTGTGCTATGAGGTGGAGCGCCTGGACAATGGCACCTGGGTCCTGATGGAC CAGCACATGGGCTTTCTATGCAACGAGGTGACCGACCCAGCCACCTGCATCCAGGCAGGGCCCTCCCAAT CCAGGGACACTCATAGATAGAAGGTTCTGGGTGGTGCCTGTGGTTTCCTGCAGTGTTTGTCACTTGTGCT TCCTGCAGCTGCTGCTGCTTGGCCCTGGAGTTGGGGGGAGACTTTGGCTTCAGTGACTCTCCATGGCCAG GTGGGGTTGACTCTGCCCAATGGCAAAGTGCTTCCTGAGGACCCTCCCAGGATCCCCTCACAGACACAGC TCTCACCAGGAATGATTCGGAACTGTGGGATTTGAGGACTCAGGGCCTACCTGACTCACAAGGCCAGGAT GTCCCTGTGCCCTCTCTTGGGCTTCATCCTGCGGAGAGACAGACTGGGGCAGGAGAGGCTGATCAGGGGT TTTGTCCTGCCCAGGGTGGAGCCCACAGCAAGGCCAGAACAGGTCCCATGTCAGGATGCAAAGATGGCCA GCAGTTGGGGAAGAACTGGGCCAGGAAAGGCTGAGGGGGCCAGGAAAGGCTGAGGGGGCCAGGCTAGGCT GGCCAGGAGCCCTGAGCCCAAGGGACTTTCTTCCCTGGCCCCTACCCAGCACAGCCTCTGTCTGGAGAGG CCAAGTTCTGCTTGGCTCTGTTGCTGGAAACTGGGGGCTTCTCTGGGCTCTATGAGCTCAATGTGGGCCT CGCTGGATCCACACACACTGCTTGGAACATCCTTTCAAGGGTGCCAGTCTCCATCACCGCCTAAGCAGTG GGACTCCCCCAGGAATGACCCACAGCCCCTTCTCAGCACAAACCATGTCATCCACTTCACCCTGCATCCC CTCTGATGGAATCCCTCCCTGTCTTTGTCCCCATCACAATCACAGTCCTTGCTGCCCTGCTGTCCCCAAC TTGACCGATTCCTAGTCTTAGTGGAAAATCCTTTTCTCTGAAGTTTGGAGTAAATACCATATTTCTATAA GTCAAAATAATGACAATTATACCATGTCACAGGGACCTTCCCACATTGGAGTGAGTCCTGCTGGTCTTCT CTTCCCCACTACTTTTGGTGGAATTCATTTTGGCCTAAGTCTATATCACATTTGGGCTCAGCACAGGTTG TGGCACAGAATAGAAGTTTCTAGAATTATGGGCCACAGATGGGATGGGAGTAGGGGAGTTGATGAGTAGA GCAGGTCAATCTCCCCTTGAAGGAAGCACACTCACTCAGGGGCATCAGGCCAATGACCTTGGGGCTCCGC CGGCCCCTCCTCCCTGCCCCCATCTCTGTAGCCCCTCCCTGCAGGGCTGGGTCTGGGGTGAGGGTCCTGA AGGCTCTGACCTTGGGTACAAAATTGATGGGGGCTCCAAATGCTCAGTAATTAGAGAAAACACATTTTAA TTTAATAATTAATGCAATATTTTAAAACGCAAATTTAATACAAATAGATCATGATGAATAAAAGCAAAGA TTTTAATAAAGCCTGGCAGGAGAGTGCTGTCCTGTGTATATTGGAGCTGGGGCAAAATAAAACCTCATTG CTCCTGATCTGTTTGTATTTAAATGGGCAACATTTTGCTTATCTTGGGATTTTTGCATAACATCTGATAT TTTTAATATTGCATAGAAAATCATGCATCCTAATAACCGGGGTTTTGGCGACCCCTCTAATTTCGTGCTG GAGGCCCCCGCCTCCCCAGCCCCACCTGGTCCAGGCGCTCCCTCCCTGTTCACCACACATCACCTCACAC TCTGTTTCCTTTTCTAGGCTAAGAATCTTCTCTGTGGCTTTTACGGCCGCCATGCGGAGCTGCGCTTCTT GGACCTGGTTCCTTCTTTGCAGTTGGACCCGGCCCAGATCTACAGGGTCACTTGGTTCATCTCCTGGAGC CCCTGCTTCTCCTGGGGCTGTGCCGGGGAAGTGCGTGCGTTCCTTCAGGAGAACACACACGTGAGACTGC GCATCTTCGCTGCCCGCATCTATGATTACGACCCCCTATATAAGGAGGCGCTGCAAATGCTGCGGGATGC TGGGGCCCAAGTCTCCATCATGACCTACGATGGTAAGAATGGAAGGTTCAGGTGGGGTGGGGTGGGTGGG GGCAGGAGAGGTTCCTGGGAAGAAAAGGAGAAAGGCCTTGGTCTGCTGCCTGCAGAAACGATGGCTGGAC TCTGGGACCTGACTTTGGGGTCGATGGGAAGAGAGAGGCCAGGCCAGGAGATATGGGCCCGGGGAGGGTG GCTGGAAGTGGAAGCAGAACTTGGGGATTTCCGAAAGAAAGAGAACTGGGCTGGCCCAGATTCCAATGGG AAGGAAGTACCTGATGAAGGAGCTAAGTCCCTAGGGGAGGGAGAGGGAAAGGAGGGACTGAAACCAGGAT GTGGGAAGTCTGTCCTGAGAGTCATGGGCCCTTGGTGCTGCCCCCTCCCCACAACAGGAGCGTGACTTAT CTCCCCTGTCCCTTTTCAGAGTTTGAGTACTGCTGGGACACCTTTGTGTACCGCCAGGGATGTCCCTTCC AGCCCTGGGATGGACTAGAGGAGCACAGCCAAGCCCTGAGTGGGAGGCTGCGGGCCATTCTCCAGGTGAG GGCTTCCTCCCTCTGCCTGGTGCCCCATCGGCCTCCCCCTCCTCCCCGCTCCCCTGTGCCTTGCCTTCCC CTCTGCTCAGAGCCTCCTCTGGGTTCCCTGCTCCCCACAGGGCGCCCAGCTCCGTCCCTCCCTTTCCTTC TCACAGCCTCCTTCTCTTTCCCACCTCCCGCATCCCTCCCTCCTCTCCCGTCATTGTCACTGTCCCCAGG CCACCTCCCTGTGCCCTCTTTCCACTCTCTCACCTCCTGCTCCATTCAACCCCCCTGCTCTTCCAGAATC AGGGAAACTGAAGGATGGGCCTCAGTCTCTAAGGAAGGCAGAGACCTGGGTTGAGCAGCAGAATAAAAGA TCTTCTTCCAAGAAATGCAAACAGACCGTTCACCACCATCTCCAGCTGCTCACAGACACCAGCAAAGCAA TGTGCTCCTGATCAAGTAGATTTTTTAAAAATCAGAGTCAATTAATTTTAATTGAAAATTTCTCTTATGT TCCAAGTGTACAAGAGTAAGATTATGCTCAATATTCCCAGAATAGTTTTCAATGTATTAATGAAGTGATT AATTGGCTCCATATTTAGACTAATAAAACATTAAGAATCTTCCATAATTGTTTCCACAAACAC >gi|22907025|ref|NP_004891.3| probable DNA dC->dU-editing enzyme APOBEC-3B [Homo sapiens] MNPQIRNPMERMYRDTFYDNFENEPILYGRSYTWLCYEVKIKRGRSNLLWDTGVFRGQVYFKPQYHAEMC FLSWFCGNQLPAYKCFQITWFVSWTPCPDCVAKLAEFLSEHPNVTLTISAARLYYYWERDYRRALCRLSQ AGARVTIMDYEEFAYCWENFVYNEGQQFMPWYKFDENYAFLHRTLKEILRYLMDPDTFTFNFNNDPLVLR RRQTYLCYEVERLDNGTWVLMDQHMGFLCNEAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFIS WSPCFSWGCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFEYCWDTFVY RQGCPFQPWDGLEEHSQALSGRLRAILQNQGN Vaximmutor bfrA Mycobacterium tuberculosis H37Rv 885767 15609013 Rv1876 AL123456 NP_216392 83332 2125339 2125818 + bacterioferritin BfrA 4.23 17744.75 159 Also known as bfr >NC_000962.3:2125339-2125818 Mycobacterium tuberculosis H37Rv, complete genome CATGCAAGGTGATCCCGATGTTCTGCGCCTGCTCAACGAACAATTGACCAGCGAGCTCACCGCTATCAAC CAATACTTTCTGCACTCCAAGATGCAGGACAACTGGGGTTTTACCGAGCTGGCGGCCCACACCCGCGCGG AGTCGTTCGACGAAATGCGGCACGCCGAGGAAATCACCGATCGCATCTTGTTGCTGGATGGTTTGCCGAA CTACCAGCGCATCGGTTCGTTGCGTATCGGCCAGACGCTCCGCGAGCAATTTGAGGCCGATCTGGCGATC GAATACGACGTGTTGAATCGTCTCAAGCCAGGAATCGTCATGTGCCGGGAGAAACAGGACACCACCAGCG CCGTACTGCTGGAGAAAATCGTTGCCGACGAGGAAGAACACATCGACTACTTGGAAACGCAGCTGGAGCT GATGGACAAGCTAGGAGAGGAGCTTTACTCGGCGCAGTGCGTCTCTCGCCCACCGACCTG >NP_216392.1 bacterioferritin BfrA [Mycobacterium tuberculosis H37Rv] MQGDPDVLRLLNEQLTSELTAINQYFLHSKMQDNWGFTELAAHTRAESFDEMRHAEEITDRILLLDGLPN YQRIGSLRIGQTLREQFEADLAIEYDVLNRLKPGIVMCREKQDTTSAVLLEKIVADEEEHIDYLETQLEL MDKLGEELYSAQCVSRPPT Protective antigen Mycobacterium frA (or called MMP-II) is one of the immunodominant antigens [Ref3014:Mukai et al., 2014]. bfrB Mycobacterium tuberculosis H37Rv 886176 15610977 Rv3841 AF102693 NP_218358 83332 4314177 4314722 + bacterioferritin BfrB 4.47 18854.76 181 >NC_000962.3:4314177-4314722 Mycobacterium tuberculosis H37Rv, complete genome TATGACAGAATACGAAGGGCCTAAGACAAAATTCCACGCGTTAATGCAGGAACAGATTCATAACGAATTC ACAGCGGCACAACAATATGTCGCGATCGCGGTTTATTTCGACAGCGAAGACCTGCCGCAGTTGGCGAAGC ATTTTTACAGCCAAGCGGTCGAGGAACGAAACCATGCAATGATGCTCGTGCAACACCTGCTCGACCGCGA CCTTCGTGTCGAAATTCCCGGCGTAGACACGGTGCGAAACCAGTTCGACAGACCCCGCGAGGCACTGGCG CTGGCGCTCGATCAGGAACGCACAGTCACCGACCAGGTCGGTCGGCTGACAGCGGTGGCCCGCGACGAGG GCGATTTCCTCGGCGAGCAGTTCATGCAGTGGTTCTTGCAGGAACAGATCGAAGAGGTGGCCTTGATGGC AACCCTGGTGCGGGTTGCCGATCGGGCCGGGGCCAACCTGTTCGAGCTAGAGAACTTCGTCGCACGTGAA GTGGATGTGGCGCCGGCCGCATCAGGCGCCCCGCACGCTGCCGGGGGCCGCCTCTA >NP_218358.1 bacterioferritin BfrB [Mycobacterium tuberculosis H37Rv] MTEYEGPKTKFHALMQEQIHNEFTAAQQYVAIAVYFDSEDLPQLAKHFYSQAVEERNHAMMLVQHLLDRD LRVEIPGVDTVRNQFDRPREALALALDQERTVTDQVGRLTAVARDEGDFLGEQFMQWFLQEQIEEVALMA TLVRVADRAGANLFELENFVAREVDVAPAASGAPHAAGGRL Protective antigen C19orf66 Homo sapiens 55337 154350198 AC020931 NP_060851 9606 19 10196805 10203927 + chromosome 19 open reading frame 66 7.28 31422.73 291 Also known as FLJ11286; FLJ40743 >gi|224589810:10196805-10203927 Homo sapiens chromosome 19, GRCh37.p5 Primary Assembly CGACTGCAGAGGCCGCGCCGCCTCCCCCGTCCGAGGTCTGCGCGCTCCGCCGCAGGGTGCAGACCCGGGG CGCCCGCCTGGGTTTGGGGCGCAAGAGCAGAGGCGGAGCCAGGGCGGAGCCAGCGCGCCGGGTCCCCCCT GAATCGAAAGCGAAACAGGGGCCGGGGAGGAAGGGCGGAGCCGGCCCCGAGGCACCGCCCCCTGCCCTGC GCGGCTGCTGGACCGACGGGCGCACCCAGGTAGGGGGGCGGCTGAGCCGCGCAGTGCGGACCCTCGCGGG GAACTGCGCCGCCGCCACCATGTCTCAGGAAGGTGTGGAGGTAAGCGATGGCTACGGCTGGGCCGGGGTC AGCCGCGACAGACCCCGAGGAGCGGCCGGGAGGCGCGGAGGGGGCTTCGCAGTTCCTGGGGACCCCCATC CTAGAACCCCAGATCCTTACCCCTGCCTGGCGCTCGCCCCCCTTGAAAAGGAAAGTGACTCCCACGTTTC CCCAGAGCGAAATGAGGCCTCCCCGAGGAAAAGCGGGGGCTGCAGGGTCAAAGGTCAGAGGCCAGAGATA ACCTGGCCGCCCCCCCACACCTTAGGCTGGGACGCTCGCCCCAGTCCGCGCCTTCCCCCAACGCCCTGTG GGGACTTTGGCTTTTTCCAGGAAATGCCGTAAAGGGATGAAAGGCGGGGGGGGGGCGGCGGAGGCCAAAA CCAAGGGTCAAGTTCGGGCCGGGAGAACGGTGCCTAGAGATGGGGGAGGGATGATCCCGTTTCCCCTTCC CCCACCGGAACCCCCCTGTCTCCATCCCAGCTGGAGAAGAGCGTCCGGCGCCTCCGGGAGAAGTTTCATG GGAAGGTATCCTCCAAGAAGGCGGGGGCTCTGATGAGGAAATTCGGCAGCGACCACACGGGAGTGGGGCG CTCCATCGTGTACGGTGAGGCTGCAGGGGTCCCGGGCCTGGTGCGGGGACCGCGCGTGGGAGCGCCGAGG GGGCGTGGTCTAGGGAGAGGCGTTGAGATCACCTCCCCCGGAGGTCCCAGCCTTGGGGAGGCTCTGCGTC GCGGCTCTGGGTGGCCTGGAACTCCCACAGACCCTTCAAGGGCCCTTCCCTTATATGCACTCTCCCCCGC AGGGGTAAAGCAAAAAGATGGCCAAGAACTAAGTAACGATCTGGATGCCCAGGTAACCTATCCCCTCCCC TCGCAAAGGACCGGGTCACGGGAGGGAGAGCAAGAGGGGGGACCCGACCCGTTCATGGTGACTGACCCCC CTCTGAAACAGGAGGGACCAGGCATTGTCGGTCCATAACTCATCACCCAATCTCACTTTTTCACCTCTCC ATCATTTGTCCGGCCAGGGAGCCTGCAAACAGTAGGCGCTCATTATGTGCTGATTGTATTCAATGGGGGA GAGAGCCATGAAACTAAGCAATGAAAGGAGCCAGGATTATTGCCATCATAGCGGGGGCGCAGAGGTCAGA GAAGGCTTACTGGAGGAGGAGGTACCAAGTCTTAAAGGATGGGGAGGAGTGAGCCCTGGGAGGGGAAAGG GAAAGAAAGGCAGGGGTCTTGGGGTGGGGGAGGGAATGGCATAAGCAAAGGCTGGGAGGTGGGGAAGGGT TGTTAGTGGCAGTCTCTAGAAAGGCTAGGATGAGCAGAGGATTGGGGAGGAGAGAGAGGCAGGGGAATGG AACCCTTGTGTTGAATGAATGAATGAATGAATGAATGAATGAATGAATGAATGGCTTTGAAGCACAGGTG CATCATGGCTACTTTGCTTCTTTGACAGCTTTGGTGATGGGAACGCTGTGGATTTAGCCTGCCTGAAAGG CTTAGTGTAGGGCTGTTAAAGAGTATAATAGGCCAGGCGCGGTGGCTCACGCCTGCAATCCCAACATTTT GGGAGGCCGAGGCGGGCGGATCACCTGAGGTCGGGAGTTTGAGACCAGCCTGACCAACATGGAGAAACCC GTCTCTACTAAAAAATACAAAATTAGCTGGGCATGGTGGCGCATGCCTGTAATCCCAGCTACTCGGGAGG CTGAGGCAGGAGAATCACTTGAACCCAGGAGGCGGAGGTTGCAGTGAGCTGAGATCACACCATTGCACTC CAGCCTGGGCAACAAGAGCGAAACTCTGTCTCAAAAAAAAAAAAAAAAAAAAAATGCCGGGCGCGGTGGC TCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCGGATCATGAGGTCAGGAGATCGAGACCAT CCTGGCTAACATGGTGAAACCCCATCTATACTAAAAATACGAAAAAAAAAATTAGCCAGTTGTGCTGGCA GGCCCCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTG CAGTGAGCCGAGATCGCACCACTGCACTCCAGCCTGGGCAACAGAGCGATTCTCCGTCTCAAACAAACAA AAACAAACAAAACCAGAGTATAACAAAACATGTATCATAGTCTACATTTATACATATAAAAGTATGATAA AAATGATAGCAGAGGGGCCAGGTGTGGTGGCTCACGCCTGTAATTCCAGCACTTTGGGAGGTTGAGGCGG GCAGATCACTTGAGGTCAGGAGTTCGAGATTAGCCTGGCCAACACGGTGAAACCCCTTCTCTATTAAAAA TACAAAAAATTAGCTGGGCGTGGCAGTGCATGCTTGTAATCCCAGCTACTTGGCAGGCTAAGGCAGAAGA ATCACTTGAACCTGGGAGGCAGAGGTTGCAGTCAGCCAAGATTGTGCCATTGCACTCTAGCCTGGGAGAC AAGAGCGAAACTCCGTCTCAAAAATATAATAATAATAATAATAATAATAATAATAATAATAATATTAGCA GAGAGTAGCATTCATTGAGTACTTCCCACATGGCAAGATTCCAGGAATTTATTTATTTCATCACTGCAGT GACCCCAGGAGACAGGTCCTATTATTTATCCCCATTTCACAGACAAAGAAACTGAGGCCGAAGTCACTTG CCAGCAAGCAGGGGCAGCTGCGATTTGAACCCTGGCCACCTGGAGTCAGAGCCTGGGCTCTAACATGCCT GTCAGTGGGAACCCAGGGGGCAGGGAGTGGCTTGCCCAAGTCCCCACAGCTGGAAGTGATACAGCTGGGA TTTGAACCCACATCTCTCTCCCACTTAGCCGAGCACAACGTGGCCTCAAAGGGGCGATATGTCACAACAT CAACAAGCATGTGGCATTTGCAGGCAGGAAGTGAGTATGGGGGTCAGGCTAGCCTCACTCAGGCAAGCCC TCCCCTAAGCCTCAGTTTCCCCATCTGAGCAATAAGGTAACAGGTGTGCCCACCTCTCAGGGCTGCTATG AGGACAACTGAGGCCACACACCTGGCTCTCAATAAGTGAGTGGTGCTCTGTGAGCTTCTGGCGAGTTAGG GGCACCCTACTCATTGCGGCCGGGGGCCTCCCCCAGCAAACGGGTGGAAAGAGGCCTGAGCCCCATCCCC AGTTTCTGCCCCTGCTATCTCCACCCAGGATCCACCAGAAGATATGAAGCAGGACCGGGACATTCAGGTG AGTTGGTGGCTAGGGCTTGGGATGGGGGAGTTGGGAGGGGGACATCTGCAGGCACAGAAGGATGTCCATT AGGGCAGGAGGGGAGATATTCACTGGGGCAGGAAGAGGACTCGTCTGTAGGGAGAGGAGGGCTGATGCTT ATGTTGGTGCAGAAGGGGTGACACCCCCCCACCTCATCCCCACCTGCCCCATTCCACAGGCAGTGGCGAC CTCCCTCCTGCCACTGACAGAAGCCAACCTACGCATGTTTCAACGTGCCCAGGACGACCTTATCCCTGCT GTGGACCGGCAGTTTGCCTGCTCCTCCTGCGACCACGTCTGGTGGCGCCGCGTGCCCCAGCGGAAGGAGG TGATGACCTAAAACTTAACCTCGACTTTGACTGTCCCGAACTCCACTCCTGACTCCTATCCTCAGTGACT GTACCTCTGACCTAAGGATTTCAATCACTGAGAGTCCAATCCCTGACCTCCAAACTCAGAGCTCAGCCTT GATCTCTGATCCCAACCCCTGACCCAGGACTGGACTTCTGACCTTAAATGTGAGAAGCCCAATACCTCCT TACCCCAGAACCTGACCCTTGACCCACAATTGAACAGCTACTACAGAAATCAAACCTGGCCAGGCGCAGT GGCTCATGGCTGCAATCTCAGTGTTTTGGGAGGCTAAGTAGGGAGGATCGCTTGAATTTAGGAGTTTGAG ACCAGCCTGGGTGACAGGGTGAGACCCTGTCTCCAAAAAAAAATGTGTTTTGAAGACAGGGGGTCTCGCC CTGTCACCCAGGCTGGAGTGCAGTGGCGGGATCATAGCTCACTGCAGCCTCCACCTCCTGGCCTCAGGTG ATCCTCCCACCTCAACCTCCCAAGCAGCTGGGACTACAGGCCTGCGCCACCACACTTGGCTAATTTTTGT ATTTTGTGTGTGTGTGTGGAGATGGGGTTTTGCCATGTTGCCCAAGCTGGTCTCAAACTCCTGGGCTCAA GCAATCTACCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGTGCCTGGCAAAAAAAA ATTTTTTTAATTAGCTGGGAGTGGTGGTGTGTGCCTGTGGTCCCAACTACTCAGGAGGCTGAGGTGGGAG GATCGTTTGAGCCTGGGAGGTCGAGGCTGCAGTAAGCCATGATCACAACACTGCATTCCAGCCTGGGTGA CAGAGCAAGACTCTGTCTCTAAAAAAAAAAAGAAAGAAAGAAAAGAAACCAAATCTGACCTCAAAAACAC AGCCCCAAATCTGGCTTCTGAACCTCCTGACTAGTAGTTCACTTCTGGTCAATGCTTAACTAGTTCTTGC AAAAGAGGCAGGAAGCCAAGATGTGTAGTGTTTGCCAATTTCTGTGGTGTAAATATTCCCACCATGGTCA ATATCAGGCTACCCACGTGAAGTCACTGATTGAGTTGGGTTGCTCAAGCTGAGGCCAGCCGCTGCAGCAC ACTGCTAGCCCTGACCCCAACCTCAGACACCTCTGACAATCCTTGGTCCCCTCTCTGTACCTTCCTGCCC ACCACCACCAGGTATCCCGGTGCCGGAAATGCCGGAAGCGCTACGAGCCAGTGCCAGCTGACAAGATGTG GGGCCTGGCTGAGTTCCACTGCCCGAAGTGTCGGCACAACTTCCGGTGAGGGCGCTGACCCCCAGCTCCC CCTCAGCCCTGCCCTACCCCAGCCCTGCCCCTCCCTGCCCTGGCCCCACCCTGGCCCAGCCTCGCCCTCG GACCCTCACAGCCCTGCCCGCCCCCAGGGGCTGGGCACAGATGGGGTCCCCGTCCCCCTGCTACGGGTGC GGCTTCCCCGTGTATCCAACACGGATCCTCCCCCCGCGCTGGGACCGGGACCCGGATCGCCGCAGCACCC ACACTCACTCCTGCTCAGCTGCCGACTGCTACAACCGGCGAGGTGAGGCTCTTCTCCCCCAACAGCCTGG ACAGTCTTTGTCCCCTTCTGTGCCTTTAAGTCCCCAAATCTCCACTCAGTCCACTTTGTCTCCCACAGCA GCAGCCACTGCTTCCACATGGCCTCCATGACCCCCCAGTCTCCGTGGTCTTGCCCAGGAGGCTCTGAGGT TTCACCCCAGTGGCCCGTGCCTGAGGGTCCCCATGGCCTCTGCCCCCATGGTCTCTGGGTTTGGGGCCCC TGTTTTGTCCCCTGTAATCTCAGGTGACCCCCATGTCCCCAGGTCTCCTGTATCTTCAACACCCCTGAAT GTCCAGTTGTGCTGGTCCCCGTATCTGGTGGTCTCAGCTCCTCCCTAGAGCCCCGCGTGGCCTAAGTCCC CTCCTCCCCAGACTCTCATGGGACCTCCAGCCCCATGTCTGCAGCACCTCTCCCTCCCTAGAGCCCCACG TGCCTGGGACATCCTGTGCTCACCCCAAGAGCCGGAAGCAGAACCACCTGCCCAAAGTGCTCCACCCCAG CAACCCTCACATTAGCAGTGGCTCCACTGTGGCCACCTGCTTGAGCCAGGGTGGCCTCCTGGAAGACCTG GACAACCTCATCCTGGAGGACCTGAAGGAGGAGGAGGAGGAAGAGGAGGAGGTGGAGGACGAGGAGGGCG GGCCCAGGGAGTGACCCCTGCCAGGTGCAGATACAAACCAGACACGGTCTGTGGCTACTTTGTGTTATTA TAAGATATGAGCTCAAACCGAGATATGAATGACCTTGGGGAGCCATCTGAGGCCAAGATATTGACGGGGG GGATTCCTGGGTCCCATTTTCAGCGCCCAGGGTCACAGATCCACAGTGGGAAGTTCTGTGGGACACATTG GCACTGAGCCACAAAGAAGGTGTGGCCAGAACAACTTGGGCTCCTGCTGACCAATGTCCTCTAGGGCCTA GGGGACAGAGGAACACAGAGTCACAGCTTCAGGGGCCGAATGAGCATGGCGGCCTTCCTGAGAGAATATG CCCCACCACGAAACTCAGCCCAGTAGACACCATCCTGGTAGCGGCTTCGGTAGTGGCCGCCGTGGTGCCA CACACCGTTGAGGTTGGAGTGGGCACAGGCATGGTACCACCAGCCTCCCCGCTGGTACAGGGCACAGTTA CCTGAGGGGAGAGAGAGAGTCCATGTCCTCTCACCAGAATAAAAGCCTCTACCTGCACCTCACAGTGCAA GGCTTTTGCCAGGCATCCCCTGGCCCCTCCCATTCTTATTGAATACAAGCCCTGATCTTCCATCTCCTCA GCAAAAAAATAGGAGCCCTGGCCCCCCAACTTTCTTCAGAGTAATAGCCTTAATTCCTTCCCTATCTCCT TACCAAAGTACAAGTCACATCTTTCCCACCTTTTCTGCAAACTAGGAGTCTACCGTTCATTCCTTTATCA AAGAAAAGTATCTACTTCCTTTCTAGAATAAGAGTACTAGCTCTCACCCTCTGCCCTTTACTTGAACAGG AGTCTTGATTCTTTTTTTGCCTCATCAGAGAAGGAATCTGGACTCCCCATCCCCCCACCAGGATAAAAGT CCTGACCTTTGTTCTCTTGACGGAATAAAAGCTTGCTTATCCTTATACTTACC >gi|154350198|ref|NP_060851.2| UPF0515 protein C19orf66 [Homo sapiens] MSQEGVELEKSVRRLREKFHGKVSSKKAGALMRKFGSDHTGVGRSIVYGVKQKDGQELSNDLDAQDPPED MKQDRDIQAVATSLLPLTEANLRMFQRAQDDLIPAVDRQFACSSCDHVWWRRVPQRKEVSRCRKCRKRYE PVPADKMWGLAEFHCPKCRHNFRGWAQMGSPSPCYGCGFPVYPTRILPPRWDRDPDRRSTHTHSCSAADC YNRREPHVPGTSCAHPKSRKQNHLPKVLHPSNPHISSGSTVATCLSQGGLLEDLDNLILEDLKEEEEEEE EVEDEEGGPRE Vaximmutor CD70 Homo sapiens 970 4507605 AC008760 NP_001243 9606 19 6346586 6351901 - CD70 molecule 8.6 19828.21 193 Also known as CD27L; CD27LG; TNFSF7 >gi|157718668:6346586-6351901 Homo sapiens chromosome 19, alternate assembly HuRef, whole genome shotgun sequence CTGATTTTTGTATTTTTTAATAGGAAAATGAAAGAAAAAGCTCAATGCCTTCTCTTGTCCTGCCACCACT ACCCCCCACCACACTCCCACCCCAACCCCGGGTGGCCCCTGTGTGTACACTTTTTCTCTTGAACTTAAAT AAAATAAAATAAAATTTAAAAAACCCTAATCAGCAGCAGTGGTCAGGGGCGCACCCACTGCACTCCAAAG AAGGTCTCATCAGTGTTTCGGGAAGGCAAAAGTGTCCCAGTGAGGTTGGTGCAGAGTGTGTCCCCTCGGG CCAGGGGCGTCAGGCGCTGGGAGGCAATGGTACAACCTTGGTGGAAGCTGAGACGCAGCAGGCTGATGCT ACGGGAGGCGGGAGAGCAGATTCCCACGGCCAGGGTGGTGGGGTGGTGCCTGGAGGCCGTCGTGGAGGAG CAGATGGCCAGCGTCACCTGGATGTGTACCATGTAGATGCCATCACGATGGATACGTAGCTGCCCCTTGT CCAGCTCTGGTCCATGCAGGAAGGAGCGGCCCAGTGCTGGGCCCCCCTGCCAGTATAGCCTGGGGTCCTG CTGAGGTCCTGGGGGCACAGGGTTAGAGGGATGAGAGGATGGAGGTTTAGGGAAACTGAGGCACAGGGGG TCATAGAGAAAGTCAGATATAGAGATCGAGAGTTAGAGATCAAGGAATAGGTTGGACGTGGTGGCTCATG CCTGAAATCCTAACAGTTTAGGAGGCCAAGGCGGGTGGATCACCTGAGGTCAGCAGTTCAAGACCAGCCT GGACAACAGGGCGAGACCCCATCTCTACAAAAAATAAAAAGTCAGCCAGGTGTGGTGGTGCACGCCTCTG GTCCTGGCTACTCTTGGGAGGCTGAGGCTGGAGGATCGCTTGAGCCTGGGAGGCTGAGACTGCAGTGAGC TATGATCACACCACTGCACTCCAGCCTTGGTCACAGAAACCCTGTCTCAAAAAAAAGGGAGGGGGATAGA AGGTTAGAGATCTAAAGGGAAGGAGAGAGGGGTAAGGGGAAAGGGGTAGAGACAGGGAGAGAGAGACACC AGGAGAGAGAGAGACAAAAAAAAAAAAAAAAAAAAAACAGAAACAGACAAGGGTAAAGATAGAGGGGGAG GGAGAGATATATAGAGAGAGAATGCAGAGAGAGAGCAAGAGAGAGAGTGTATGTGTGAGAGAGGACGAGA GTGCACGAGAGAGTGCGACAGAGAGCATGAGAGAGAGAGCATGAGAGAGCGAGAGAGTGTGTGAGAGGAC GAGAGAGCTTGAGGGAGAGTGCACGAGAGAGTGCGAGAGAGCACGAGAGAGCAGGAGAGAGTGCAAGAGA CAGCATGAGAGAGAGAGAGATCATGAGGGAGAGAGAGCATGAGACAGCGAGAGAGTGTGAGAGAGAGGAC GAGAGAGCTTGAGGGAGAGTGCACGAGAGAGTGAGAGAGAGCACGAGAGAGCAGGAGAGAGTACGAGAGA CAGCATGAGAGAGAGAGCAAGAGAGAGAGTGTGTGTGTGAGAGAGGACGAGAGAGCTTGAGAGAGAGTGC ACGAGACAGAGTGTGAGAGTGAGCTCGAGAGAGTGCATGGGAGAGTGTGTGTGTGAGAGAGGATGAGAGC TACAGAGCTCGAGAGTGCACGGGAGTGAGCGAGAGAGTGCCTGAGAGAGTGAGACAGACAGCACGAGAGA GACAGCGTGCGCACGAGACAGCGCGCAAGAGAGTGCACGAGAGAATTCCCCAAGGGACGCCTGCTCGGGT CACACCCTGAACGCCAATAAAAGCTTTGGCTCCCAGGACCCTTTTTTTTTTCTTGCTTTCCACCCGCTGG TTGAGTGTGTGTCTCAGAGAGCTCCCCTACCTTCCCGTTGGCCCTCTTCTCTCTAGGATCTTTATTTTCT TTAGAGACAGGGTCTGGTCCTGTCACCCAGGCTGGAGTGCAGTGGTGCAATCATAGCTCACTGCAGCCTC AGCCTCTGGGGCTCAAGCTATCTCCCACCTCAGCCTTCCCGAGTCACTGGGAGGTGGCATGCGCCACCAT GACCAGCTAATTTTCAAAATTTTGTTCTAGAGATGGTGGTCTTGCTATGTTTCCCAAGAAACATAGTCTA GAACTCCTGGGCTCAAGCGATCCTCCCACCTCAGCCTCCCAAAGCGCTGGGATCACAGCCGGGAGCCAAG GTCCTCGGCTATATTTCACGTGTTTCATCTCAGCGTCTCCTGTCTCCGCAACTTATCTGACTGACACACC TGAGCCTCACTCTCCTCCTCACTCAAGGCTATAGTGGAGACTGGCTATCTTGTTAGGAAGGAACTGACAC GGGGTCAAACAGGAGCCCCAAGGGTGTTTACCAGGCTTTGCAAATTTCCTGTAAGAGTGACACCTGGTCC CCTGTCAGACACTTAGGCATGATATCATCCACCAGAAGAAAGAACTGTCCTGTGAAAGACACTGTCAACA CCTGTGGCCACCACCCCTGGTGCCCCATCGATGTGGGGCTGGACAGCCACTCTCCAGAAAGGGGCCTCAA GACCAAATTACAGGAAAAATATGGCAAAGGCCGGGGAGGGGGGGAAGATATATCAGGGGTCAGAGACCCA AGGAGAAGAGAGAGCAACAAAGGTCCCTTCCATTACAAAATGGAAGGAACAAAACACGCAAGCGTGCAGT AATTTTAGTCTCTGGGGATGAACCGTGGAAAAACAGAAGAGGAAGAAGCGTTCGAGAGAGGCTGGGAGGA GGTAGAGACTTCCACCTAGAGCAGAACTGTTCATTTCTTCTCCCTCAAGGCTTCTTGCCAATGCCACCCA CTCAGATACCACCCCCCCACCCGACCGCCGCGACTCCACTTTAAATGAAAATCCCCAACCCTGGCATGCC CGGATTCCCCACCCACCTCCTTGCCTGATTTTTTCTCTAAAACCTGCTCTTGGCCTGTTTTACTCTACGT GGCATTGCTCTGTTTTTCACATGTTCGTTTTTATTTTTTTGGTCATCTCCTCCTCGGTAGGAGTGATAGT AGAAGGGAAGCTCACCAGGCAGGGATTTCTGTCTGTTTTGTGCGCGACTGCAGTACCGGAACAGTGCCTG CCCACTGCAGATGTATAATTAGTGCTTGTAGCATGAATGGAGGAAGGGTAGCCTTTCCTGTGAGCGTTTT CGTTTACTAACAAAACCCCTTTGATTCTTCCAAGGGAGGAGATCGAATGTGAAGGACTGAGAGGGAAAGG GAGAGAAAGGTAGTGGATGCCCAGAAACACAAACCTGCTCGCTCGCTCTCTCCTCTCTCTCTCTCTCGTT CTCTCCCTTTTCCTTTTTCTCTTGGGAATGATCTCTTTCTCCCCCGCCCTGTTTTTCTTCTTCCCCCTCT CTGTCTCTGTCTCTTCCTCTCTGTTATTCTCCTTCTCTTTTCCTCTCTCTGGCTTTCTTTCTTTTCTTTC TCTTATTTCTTTTTTTCTCTTTCTTCTTTCTTTTTCCTTTCTCTCTCTCTCACCTCCCTCCCTCTCTCCT TCCTTCCTTCTTCTCTTTCTTTTCCTTCCTTCCTTTCTTTCTTTTTCTTTTCCTTTCTTCTTTTTATTTA TTTATTTGACAGAATCTCCCTCTGTCACCCAGGCTGGAGAGCAGTGCCATTATCTTGGCTCACTGCAACT TCTGCCTCCCAGGTTCAGGCGATTCTCCTGCCTCGGCCTCCTGAGTAGCTGGGATTACAGGTGCCTGCCA CCACACCCGGCTAATTTTTGTAATTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAA CTCCTGGGCTCAAGTGATCCACCCGCCGCGCCCTCCGAAAGCGCTGGGATTACAGGCTCCAGCAACCACG CCAGGTCGCCTCTCTCGTTTTTATTCTCTCTTCCTCTATCTGTCTTTTTTCTCTTTCTCCGTCTCTCTCC CTCTCTCTCGTTCTGTCTCCCCCGCCTGTCTCCCTCCCTCCCTCTTCCACTCTCCCTGCGTCTCTCCCTG TTTCTTTCTTTCCCCTTCTTCTCCTGTCTCCGTCTGTCCCCTCTGTCCCTCTCTCTGTTCTTTTTTCTGT CTCTCCCTCCCTTTCTCTGGGCCTCTCCCTCCCTCTCTGTCTTCTGTCTCCCCCTCTCCGTTTCCCTCCC TATCTCTCCCTCCCTCTCTCCCTCCGTCTCTGTCTGTGTCTCTTTCTCTCTGTCTCTCCCCACTTGTCTT TCTACCTCTCCTTCCTTCTCTCTCTGTGCCTCTTCTTCTCCCCGTCCCTCCACGTCCCCCGTGTTACCTG TGTGATTCAGCTGCAGCTCAGCTACGTCCCACTGGAAGAAAAGACCAGAAAACAGGGCACGGACGTAAGC AGAGAGGTTCTATGTGTCCCCTGTGCCAGGAGCTCTCTTTTCTCTGTCCATCCTCCTTTCCACCTCTCAT CCCACGGCGCGCACTGGTGATTTTATTTCATTTTATTTTTTTTTACTCTTAAGACTTCTTAAAGAGGAAG CAGGTCTGAACCAGCGGGGAACGTGAAGTCGAGACCTTCAAAAGAACTTCTTCGTTTCCCGAATCGTTTT CCTGGATGTCACTGGGCGCGAGAGCACCGCGCGCAGCGGACTCTCTACGCTGCAAAGGCGCCCGGCGCGG TCCCCAAAGGGACCCCTCCCCCACCCGGAGCGAGTGGCTGGGGCCATCCCGTCCTAGGAGGCCCCAGGAA GCCCCCACGCCCAGCCTTGTGAAGCCCCCAGCAGCCTCTGAGTCAGCCTGCCGGGGGAACACCAGAGCCG AGTCATCTTCCATTTCCATGTGTGCCTACCTTTCCCATCCCGGGATCTCCCTGTACCTCCTGGCAGGGCT GCCTGTCTCGTAGCCCCTACACCGGGCAGCCTGGTCCCCGCCTCGCCTCCCTGTTCTGGTCTCTGTCTCT GCCCTACCAGATCTTCCTCTCTGGCCTCTTTGTACCCATCTCATTCTGTCTTTTCGGTCACGCGCCTCTC TATGTTTTCTTCCCAACTTTTCCATCTCAACTCACCCCAAGTGACTCGAGCGGCAGCTGCTGCTGAGCCT GTGCGAAGCGCTGGATGCACACCACGAGGCAGATCACCAAGCCCGCGACCAATGGGACCAAAGCAGCCCG CAGGACGCACCCATAGGGCCTGCGCCGCACCGAGCAGCCCGAACCCTCCTCCGGCATCGCCGCGGCGATC ACCTCCGCTAGCGCAGGAGGGGCGATGGGGGCGCGGAGCGCTGCCGAGAAGGAAGGAAGGAAACTGCAGC CCCCTCCCGGGGCTCCTGGGCGTCTACTTGCTTCAACCTGTCAGGGGACCAGCCTGCCCCTCTCTG >gi|4507605|ref|NP_001243.1| CD70 antigen [Homo sapiens] MPEEGSGCSVRRRPYGCVLRAALVPLVAGLVICLVVCIQRFAQAQQQLPLESLGWDVAELQLNHTGPQQD PRLYWQGGPALGRSFLHGPELDKGQLRIHRDGIYMVHIQVTLAICSSTTASRHHPTTLAVGICSPASRSI SLLRLSFHQGCTIASQRLTPLARGDTLCTNLTGTLLPSRNTDETFFGVQWVRP Vaximmutor cfp21 Mycobacterium tuberculosis H37Rv 885813 15609121 Rv1984c AL123456 NP_216500 83332 2227907 2228560 - cutinase 5.88 19699.07 217 Also known as clp1; culp1 >NC_000962.3:2227907-2228560 Mycobacterium tuberculosis H37Rv, complete genome ATCATCCGGCGTGATCGAGCCTGTTCGCCGCGAATGTCGCCGCCTGGCTTGTCATCCCCGACTGAACATA CGAAACATGCGCCATAATATTGCCGCCTCCGGTGCATATTGGATCGTCGGGAGCACACAAGTTTATGGTC TTAGAGCTATACAGCGGACCGATTGTCGGCAACGACCCGCCGCCCCACAACATGCTGGAGAAACCACTGG ATGGCTCGCCGAAAAGGGCGACAGCGGCGACATGATCTGCCACCGCGGGCGGCATCGCCGAGGTGGACAA ATCGATGACCGTCGCACCCTGCGAATAGCCACCAAGCACAATCCTGGTGTTCGGGCAGCTGGCGACGGTG CGCTGGATGTGGGCGCTCGCATCATCGGAACCGTTTGACGCGCTCGCGCGGTAGTCGTCGCTTGCTGGGT AGTTCACCGCGTAGACCCCAATCGACCGCCCGCCAACTTGCGAGGTAAGCGAGTCGACGAACGCCTCACC GACGTCGCCAAGACCAGAAGCCTGATGCGTGCCGCGAGCGAAAACGACCGCGATGTCCGAACACGGATCC GCATGCGCGGCACGACCGCCGGCGGGTGCGCTCACCAGCGCCAAGGTCGTCGCAACCACGACACCAACGA TGCGAACAAGGCTGCGTGGAGTCA >NP_216500.1 cutinase [Mycobacterium tuberculosis H37Rv] MTPRSLVRIVGVVVATTLALVSAPAGGRAAHADPCSDIAVVFARGTHQASGLGDVGEAFVDSLTSQVGGR SIGVYAVNYPASDDYRASASNGSDDASAHIQRTVASCPNTRIVLGGYSQGATVIDLSTSAMPPAVADHVA AVALFGEPSSGFSSMLWGGGSLPTIGPLYSSKTINLCAPDDPICTGGGNIMAHVSYVQSGMTSQAATFAA NRLDHAG Protective antigen Cxcl10 Mus musculus 15945 10946576 AC109603 NP_067249 10090 5 92346637 92348888 - chemokine (C-X-C motif) ligand 10 10.57 10391.5 98 Also known as C7; IP10; CRG-2; INP10; IP-10; Ifi10; mob-1; Scyb10; gIP-10 >gi|372099105:92346637-92348888 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J ATTTCATGTCAGTTATTATTACTTTATTACATTTTGGGTCTCTTACTACTTTCAATACAGTACATTGTCT TTTGAATGTTACATAATACAAAAGGTATTGGACGGTTTTAAAAATAAACTTTAACTACCCATTGATACAT ACTTGATGACACAAGTTCTTCCATATACAATGCAAAGCATACAAAAAATACATTAGGAATTCTACTTTGT ACAGTCGTTCATTAAATAGTATTTACACATACATTTTCAGGTTCCTCTGAGTATCTTGATAACCCCTTGG GAAGATGGTGGTTAAGTTCGTCCTTACAAACTTAAATTTGTAGTCTTACATCTGAAATAAAAGAGCTCAG GTAAACTTAGAACTGACGAGCCTGAGCTAGGGAGGACAAGGAGGGTGTGGGGAGCAGCCTGGGCATGGCA CATGGTGAAGGGCGTCGCACCTCCACATAGCTTACAGTACAGAGCTAGGACAGCCATCCCAGCCACTTGA GCGAGGACTCAGACCAGCCCTTAAAGAATTCTTGCTTCGGCAGTTACTTTTGTCTCAGGACCATGGCTTG ACCATCATCCTGCAGGAGGAGTAGCAGCTGATGTGACCACGGCTGGTCACCTTTCAGAAGACCAAGGGCA ATTAGGACTAGCCATCCACTGGGTAAAGGGGAGTGATGGAGAGAGGCTCTCTGCTGTCCATCCATCGCAG CACCGGGGTGTGTGCGTGGCTTCACTCCAGTTAAGGAGCCCTTTTAGACCTGTAGGAAGAGGAAACTGTA TTGAGCGGAATAGACTCTGCTTTCACTTTGGGAACCCAAAGACCCTGGGGCAAAAGGCCTGGTGCATTTT CCTCTGCTACACAATCTCCATGGGTCACGGGTGGGTGGGGGTTGAGGGGACAGAATGGGTTTGTGTGTTT TTTGTTTTTTTTTTTTTTAAGTGAGGAAGTGGGGGGGGGGTGGGCATTTGGCAGCTTTACCCGTGACAGG TATAATAACTGCTCCACTTCTAGACACTAGGAATGTATGACCTAAGAAGGCACCTCAGTGTCTGTGTAAA TGGAGAGACAAAGGCACATGCCGGTGCCATGAGTTTAGGGCAGATGCCCCACAGCAAAGCCCAGGGCAGG CGCTGCTCCCCAGACCACCATTTCCTGAAAAAGCAACATCAAACCTACCTTTTTTGGCTAAACGCTTTCA TTAAATTCTTGATGGTCTTAGATTCCGGATTCAGACATCTCTGCTCATCATTCTTTTTCATCGTGGCACT ATAGAAATAAGAGTGAGATACTCAGATTAACGTCAGGCCAACAGAGCTGATGGCCCTGCAGGTCCATGTC AGAGTTCAGGCACTGTGCCTTAGTTAGAGTCACAGACCCGTCCCTATCTTTCCTGGTTTCCCTCACTTAT TTACTGTGTGGTTACGGGAGGGACGTTTATCGGCAGGTGGGCTTGTACTCACATGATCTCAACACGTGGG CAGGATAGGCTCGCAGGGATGATTTCAAGCTTCCCTATGGCCCTCATTCTCACTGGCCCGTCATCGATAT GGATGCAGTTGCAGCGGACCGTCCTTGCGAGAGGGATCCCTGCAGGAAGAGAGGGGGAGAGCCAGGAAGG TCAGCACACGGTTTCATTTTAGGCACTTAAAATGGATGAGGTAGTGTTTAGCAGACCCTGTCTGATCTGT GGAGAGACGCATCCCATCGTATCATAGGGCTGAGTGTTTGAATATCTTGGTCATGGTAAAGAGAGATCTG CAACTTGTCAGTTACGAAATCCTGCATTTATGCACTGCTCACAGTATTGGTTAGGCTCAGGCAAGGAGGC ATCCGTGCCTTACCCCATCCCACTCTTTCGCCCTTTCTCCTGACTCTCTCTTCCCTCCTTCTTCCTCCTC CCTCCTCCTTTTCTCCCTTTCTTCCCTTCTTCGTTCCTTTGCTTTTATTCTATTTTTCTCTGACTTCAAC CAGCCTATGATAAACTCTGCAAGCTGAAGGGATTTCTTCCTGCTTCCCTCTTCTTTATAGGTACACACAT TAAAGCTTAAATACATGCTGTGTTTAGAACTGAGCTTAATCGTCTGACTTCTGACTTCGTTAATTAAATG GCCTTGGTGTCCCTTACCTTGAGTCCCACTCAGACCCAGCAGGATGAGGCAGAAAATGACGGCAGCACTT GGGTTCATGGTGCTGATGGGGAGGCTTCCGGAAGGTTGGCTCGGGATGTCTCTCAGCGGTGGATGAAGCG CTTCTCGAGTGC >gi|10946576|ref|NP_067249.1| C-X-C motif chemokine 10 precursor [Mus musculus] MNPSAAVIFCLILLGLSGTQGIPLARTVRCNCIHIDDGPVRMRAIGKLEIIPASLSCPRVEIIATMKKND EQRCLNPESKTIKNLMKAFSQKRSKRAP Vaximmutor Cxcl11 Mus musculus 56066 9507071 AC122365 NP_062367 10090 5 92359543 92363295 - transcript variant 2 11.02 10670.03 100 Also known as Ip9; H174; Itac; b-R1; Cxc11; I-tac; Scyb11; Scyb9b; betaR1 >gi|372099105:92359543-92363295 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J CTTTACTTTAAGATAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGAAACTCCTGGTCTGTAGACCAGGTT GGCCTTGAACTCAAGAGATCCTCCTGCCTCTGCCTTTCAAGTGCTGAAATTAAGGTGTATACCACCACAC CTAGGTAATTTTTTTTATTGAAATTTTTAACAGTACCTTTTTATCATATTTTTTTCTCATCTTCAGCTCC TCCTCACTAAAGTATATTCCTTTCTATTTTAAAATTTTGTGTAGCCCTGACTGTTCTGAAAGACCAGTCT GGCCTGGACCTCACAGAGATCCGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGCGTGTATGTTTCT AAAGAATTCTGACAAAAACTTGTGTAGCTCTAAAAAACAAAACAAAACAAAACAAAATCTAAAGGCATCT CAATGTCCAGAGAGCATGTAGTTGTTGATGATTTAATAAGTGTTATAATAGCTTACTAGAGAAACACAAT TCTTATTCTTTTCAAGGAGAGACAGAGCCAGCCATCCCTACCATTCATTCACAGTATGACCAAGGAAAAG AACAAAGTACATATTTATATATTTTTCTCAAAGGCAGACTCACAGGAAACAGACTGAAAGAGGTGCACAG ATTTTTTTTTCAACATTTTTCTTTGGTGAACAGAACCAATTTATTTTAAAAATCTATCCTTGTTATTGTC ACTGATAAAACCAGACGTACAGGAAATACACATCTTAACCCCTTAGAAGGCCTCAGGGGGAAGCCAGTGT GACTAGCTAGGAAACAAACAGTAGTGTTCTTACTAGTTTCTACAACGCAGAAATGAATCGTAGTCTTTTT AAATGAACAAACAAGACAAAATGTGGAAATGTCTTCCTAGATGTTCGTGTGCCTCGTGATATTTGGGGAA AGGAATGTTTATTTGCATGAACAATTCTTTGAGGAATGTTTTACTACACACAGAGATGCTTTTGAATCCA GCACTGGTCCGGATTGCAGTAACAATGCATCCACTGTAACCACAGAAGGTAGCGTGGAGTGTGCACGTGA CTTTTAGAGACAAATATTTTTCATAGCCAAGTAGAACAGTTTAGATGTATCGGACCTTCTAGAAAGTTCT GCAGCCTGGTAATACGTGGCTGCATGTTCCAAGACAGCAGAGGGTCAGGTTCCTGGCACAGAGTTCTTAT TGGAGGGCTCACAGTCAGACGTTCCCAGGATGTCACATGTTTTGACGCCTTAAAAAATTCTTTTTTTCTA TTGCCTGTAATGAGAAAATGAAGAGTTTTACTTACTGGGTTGTTTCGTTTCCTCAGTAATTAAGAGCTGA CCTGTAAAAACAACGCGGAGGGGTAAGGATGTGGCCAGGTTTAACCCAGGACCCCTTAACTGGGTGCTTC TGTTAGGATGGTAATGGGGTCTTCCGGCAGTCGCCTGTTCTCTGTGTTCCAGCACTCTCGTGGGTAAAGC ACTTTCCGACCTGGCCGACTGGCAACCTACCTGCATTATGAGGCGAGCTTGCTTGGATCTGGGGTCCAGG CACCTTTGTCGTTTATGAGCCTTCATAGTAACACTGGTAAGGGAGACACAAAAGAGTCTTAAACTTTGGT GGGAAAACATCAACCAAACAAAAGCACCCATACCATTGTCACCTCCAGCACATACATCACTTCAACTTTG TCGCAGCCGTTACTCGGGTAAATTACAGAAGCTTTCTCGATCTCTGCCATTTTGACGGCTTTCATCCCGG GGCCGATGCAAAGACAGCGCCCCTGTTTGAACATAAGGAAGCCTGGGACAGACCACAGTGTTGGTTAGTC AGGGCTGTGAAGTGGATGGCAGACGCATTTGTTAATCTCAACTGTGCAGACTGAAATTAAGTTTTTGAAT CTCTCTGCATTAGGCTAATTTGCTGTATCCTTGATGATTTCCTTCCTTAATTTTTGTTAATTATTTAATT TAGGTTGCAGACAGGACTCACTTGCTATTTGGACCAGGTTTGCCTCCAACTCACCACAAAGCCCAGGATG ACCTTGAACTTGAAGCACTCACTACAAAGCAGCCCCCTCCCCCCATCTTGGCATTATCCTACCTCACTCA GCTCACCCTCCTAAGTGCTGAAGTTATATTACAGTATGAGTATGCTATGAGACCGGCTCTTGATTACTTT AAATAACACCTACACAGTATACAGCAACCCAGAGAACAAAGTGGCTATTTATTTGGAATTATTTTATGAC TGGTGGAATGGGTATACAAGCGTCTTTCACAGTCGCTGGTGATCTGTTCTTTACTAAATGAGCAAATAGT TGATAATTTCTTTGATCTTTGTCTTCCCAGTTCAAATTCTAGATTAAGACTAAGTATTATGAATCATGTT GTCTCCCACAGCTGAAACTGTCTTTAAAGCTTCCAGATGCTCCCAGGGAAACCTCAAAGGTTCATTATTT CATAAGAGCAGCAAGAGTTGTAATAATGGCTTGGCTTGTCACATTAACTTCATATCTGGCTTAGCCCAGG TCTGCTGTGATAATCGTGTAAGAACCATCAGCTCACAGGAGAAACACTTCCAGGTTCAGTCATGGGACAT GTGAATAATGCTTCTATTTAGAAACAATATTCTTTGGGAAGAGCTTGTGGAGCATTGTCAGTATCCACAC TACCTTTAAGTCACTCTTGCCCTCAAGTGACATATTGATCAAGCACGTATGACATTTTTATGACCATTGC CCCAGAGAGAAATTTCCTTTTCTCTATTCTGAAAGTATTGGTCATAACAAATTAGCAGTCTCCTGGTTAA AACATTAATTTTTTCAGAATACCAGCATAATCCCACATAAGATTCTCTGAACAGGCATTGAGGACTGGAT AATTGTCAGAAAATGAAGAAATTGGGCAGGGGTGGCGCACGCCTTTAATCCCAGTACTAACAACTGTGGA GGAATACAATGGCATTGTTTACTGCCTAATATTTGTAAGTTGCTCTGACCATAATATCTGTGGTTTATTT GAAATACAAATCGCCAGGTGTGGTGGTGCACGCCTTTTATCCCAGCACTTGGGAGGCAGAGGCAGGCGGA TTTCTGAGTTTGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCC TGTCTTGAAAAAAACCAAGAAAGAAAGAAAGAGAGAAAGAGAGAAAGAGAGAAAGAGAGGAAGAGAGGAA GAGAGGAAGAGAGAGAGAAAGAGAGAAAGAAAGAGAGAGAGAGAGAAAGAAAGAAAGAAAGAAAGAAAGA AAGAAAGAAAGAAAGAAAGGAAGGAAGGAAGGAAGGAAGAAGGAAAGAAAGAAAATGAATCCATAAACTG ATCATTTTCTTTAAGGATTTCCAACATTTGCTGCGCCATTGCTAGAACTTCTTTTTTTAATTATTTATTT TTTATTAGATATTTTCTTTATATACATTTCAAATGCTATCCCGAAAGTTCCCTATACCCTCCCCTTAGAA CTTCTAATATGTGAAATAAAGTAAATTTTTAAAACTGTATATTTTAGAGAAAAAGATATTTGAAAAGTAA CACAAGGAACAGACAGACTCAGAAGCTACGGGCACCAAATACCTTGAGCAGCTGTGGCCCAGATATTGCA GCCAGGGCTATGGCTGTGACCTTCCTGTTCATCTCAGCAGCCGCTGCAGCTCTCTGCCTGGGCTTTGGAG ATCTCTCTTTGCAGAGAGCAACGGCAGGCAGGAAGGCTGAACC >gi|9507071|ref|NP_062367.1| C-X-C motif chemokine 11 precursor [Mus musculus] MNRKVTAIALAAIIWATAAQGFLMFKQGRCLCIGPGMKAVKMAEIEKASVIYPSNGCDKVEVIVTMKAHK RQRCLDPRSKQARLIMQAIEKKNFLRRQNM Vaximmutor Cxcl9 Mus musculus 17329 110626107 AC109603 NP_032625 10090 5 92321330 92328078 - chemokine (C-X-C motif) ligand 9 11.28 14046.52 126 Also known as CMK; Mig; MuMIG; Scyb9; crg-10; BB139920 >gi|372099105:92321330-92328078 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J AGACATTGAAAAACATGTTTATTTTGGAATTTAGTGCACTTTTACTGATATCACTACGCTTTCACGAAAT AGCTTCAGAGCACATATGCTAATTTGCTTTGAATTAAAAATTAGAGTACACATATGAAGTCATACTGTAG GCATATGCATCCAACATAACTATTTAGTAAAAATTACACAGGCTTTGGCTAGTCGTTATGCATACTACCC AGCTGTCAGATGCAAGGGTAGCTAACTAGTTAGGAAACAATTTTAGACTTTTTTTTTTTTTTTCCAAAGT AAATTACCGAAGGGAGGTGGACAACGTTTTGACTGTGCAATGTTGTTTGGATGGGAGTCCCACCAGCAGC ACAAAAACCACCTGCATGCAGCGCCAGGAATGGCAGTATTTGAGAAAAAGCATGCTCAAGACGGAACTTC TGCCCAGAGACATTCTTTTCTATCTAATTCATCAGACCTCCTCTCTAGAGCAGTTTCCAGCTCCCACACA GGCTAACTTGGCCCTGTCCACACTTCCACTCGCAGAATTTACTGCTTCCTGTAGCTCTTAATTCCTTTAA AAATATCTCTGTAAAGATTTTTATTTGCCGTCTGAGATTATGGGTGTATGTCATGGGGGCCTGTGTACAC CTGTGCAATGCTTGCAGAGGCCAGAAGAGAGAAATGGGTTCCCTGGAGCTGGAGTCACAGGTGGCCACAA GGCTCACGCACACGTGGGGGTGGGTGCTGGGAATGGAACTCTGGCTCCCTCTCCAGCCCTGATCTTTCCA TTTCACCACTCCTAGCCTGATGTGGCAATCCATACAATCATACACTGGGATTTCTGCAATGAGGGAGGGA AGGTGGCAAGGAAATTCATATTCACCTAAGATAAACTTGCTAAAGGATTTGGCAGCTCTTTTGTTGACTC CTAGGGAGCTTGTCCTGATCAAGAATGATATTAGAGGAATTTTGTTTTGAGTGAGGAAAGATCTAGTATT TGCCAATCAGTCAAACCTGTCTTCCATGTAAGCTTATGTTTCTAGATGGCCTGTGTAATTTTTCCTGAGC AGTCCCAAATATCCGCAAATGTCTTGAACATGTGATTCCTGTTTGTTAAAGACACGAGCCTCCTGTCACA AGGTAAGGCTCTTTTTCACCCTGTCTGGCTCTGTGCGCTGAAGATGTCTGAAGATAATAAGAAAGCTAGA TAATTTAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGGAGGGAGG GAGAAAGGAAGGAAGGCAGGCAATCAGGTGTAGTAGCACACACCTTTAACCCCAGCATTCAGGAGGCAGT AGAATACAGATATGTATGAATTTGAGACCAGCCTTGTCTACTTTGAGAGTTTTAGGACAGCCAGATATAA AACTTTCTTTAAAAAAAAAAAAAAAAAGTGGTAAGCAAGCAAGGAGGCCAAAGGTTAGTTAGGCAATTTG GAGCAGCCGGGGATTGGCCAATGCCTGGTGTGTAACAGCCTTTCTACCTCTCACACATACAATTAGGAAA TTATTCAAAACCAAGTTTTTGTAGGCACTGTGGAAGATTTAGGGGAATAGTCTGTGGTCCCTACTCTTAA AAACCCGTCTCGTTGGAAAAAGTAAAACAAACAAACAAACAAATCCAATTAGATAGCAGCGAAGTGTTTA CTTGTGAAACAGGAATAAATTTAAGAGAATTTGAGTAGCCAGAAAATGTTCTTTAAATTGGTTAAGTTTT TGCACTGTTAGTGAAGGACCTGGTCCCTGCAACACCAAGTGTTCTGCCACTAAGCTACAGCCACAACCTT CTAGGAGAGTTTTATTGAAGAGGCAAACTTAGAATAAGCTCTGAAGATGGGATCAAGTTAATAATGAAAT CCCATGGTCTCGAAAGCTACGTGGGAGGTTCTGAAGGCCAACTCTGGTGGTCTCCACTGTAGAACCCCAA AACCATTAGCACCATCTCTGAGACACAATGTGTGCAGGGGCTCTAGGCTGACCCAAATGCAGCTGCCGAG GGCCTCTCCAGCTTGGTGAGGTCTATCTAGCTCACCAGCAAACAGACAGGCCTGGGATTTGGTGACGTGA GCCTCAGAAGTCTTCGGAGGCTGCCAAATGTGTGGCTCTGGAAGCCAGACAGCTGTTGTGCATTGGATAG CTATGAAGGAAAGGGACACTTGCCCAGATCTGATGTTATCACGACTAGGCTCAAGGGCGTGATGAAATTT TGTTGGCTGTGTAGAACACAGAGTTTTTGCCTCTAGCAATTGTTACTATTGGAGTTTTGCAATTAACTTC CTAAAGTTAGTTATAATTTCAAATTAACACTTTATGTTTTGTAGTTGAGTTAATGTATTAAAAGCCTATG TATTAAAGGCTGCTCTGCCAGGGAAGGCTTTTCAGTACAATCTAAAACCCATTAAGATTCAGGGTGCTTG TTGGTAAAGTAATGGTCTCTTATGTAGTCTTCCTTGAACGACGACGACTTTGGGGTGTTTTGGGTTTTCT GTTTTTCATGTTCTTTTGATGTTTTTTCCCCCTCTTTTGCTTTTTCTTTTGGCTGATCTGCAAGAAATGA GAAAAAGGCAATGCTTTCCAATGTTGTAGTTTCTCCCCTTCGAGATGGTCCAACTCCTGAGTGTGATCTC TCCTTATCCTCAGAGCCAGCTCACGTCGCTGCTCTCTCAGAGGCGCTGGCCTCATTATCTGCTGCCCCTA CATTAAAGAAGCTACAGTTCCCTCTTTGCTTGTTTGTCTGTTTTCTGGCATCTTGCTGTCTTTCATCTTC TCCCTTCATCTCCCACCTGGGCCAAACACAGCCCTTTGGGGCTGGCTGGTAGCGTTATCACTCACTTTGC ATATTAGCCAGTGGTTCTCAACTTGTATGTCGAAACCCATTCGGGGGCACATAGCAGATATCCTGTGTCT CAAGTATTTACATTATGATTGTTAACAGTAGCAAACATTACAGTTATGAAGTAGCAGTAAGATAACTTTA TGGTTGGGGGTCACCTCAATATGAGGGACTCATGCATTAGGGTCTCAGCATTAGGAAGGTTGAGAACCAC TGAAACTGGACAAGGCTAGGCGAGTGAGTAGCCTGAAGCTTAAGGGCTAGGGTCTAGGGCAGACCCAGCA GTTAGGTGTGGGGACCCAAGCTCCCCACCACATACCCCATGGCTCTTCTCAAACCCAGCTCCTTAGCTAT CTCACAGCTCTTTTCCCTTTTTCCTTGGCTCCTTTCTCATCTCAATCCCTTTGTTCTCTGCATTTAAATT CCAGTTACATTACTTCAGACTCACTGAACAGACTTTACTCAAAACCACCAACGCCCACACTGTACTTAAT TAACTGCCTGGTAAATGTCTTATCCTCAAAGATCCAGATCAGAGATCACTTGATAGGAGAAGTCTACCTT AATACTTTCTATATTGTCTCTTGGTCAAGTCAATTACCCTTTCCATCATAATTCTCAAAGTTCTCATGTA CACACTATTTATTCTAAATGTCTCTCACCACGTCTGTTGATTTTTATACATTGTTAATGACTATCTGCTA TATGGGGGAGGGGTGATAGCTTATTAATTTTGAATCCTTAGTGTCTTACTAGTTGATGCTATTCAAAAGG ATGGAAAATGGGTCCTTAATGGGTGTGCCCAGGATCTGCTGACTTTGTGAGCTGACCTCTGAAAGAAAGC CATTTGGGTTCCTCCCCCACCAACCTTCTTTTCCCATTCTTTCATCAGCTTCTTCACATTTGCCGAGTCC GGATCTAGGCAGGTTTGATCTCCGTTCTTCAGTGTAGCACTGTGCCAAAGAAACACCAGTGAGCTTTCTC CCCCCAGTAACAACTTCATAATTCTTAGTAGAAACAGTGGAGGAAAACGTGCATTTTATTGAGTTTTCCA GTGAAACCACTTAAAATGTGGCAAAACGTCTTCCTAAGATTAACAGGTATATTTTTAAAAACTGTAGCAG ATTTTTCAGGAGAAACCTCAATTTCTAAAAATTAATTTCTCTGTATAGCTTGAGAATAATAATGGCTATT CTATATAGAATTCCTTATTTTAGTTGTCAAAGGTCAAATATATTACATACATGTATGTATATATATATTT ATATCCTTTATATATGTATGTGTATATATTTATATCCTTTATATCGTATACATACATACACACATATAAA TATATTATATAATATTATATATCCTTTAATTTCATAGTTCTGATTTCTAAGAGGCTTGGAATGGTGGTTT ATTCCTGGAATCCCAGCATTCACGTGGACTGAAGTCAGCCTTGGCTACCTAGCAAGACTCTGTCTCAAAA CAAAACAAACACGAAATTCTAGTCTCTAGAAAAGAATTACTAGGAAAGAATTAAACACAATCCTACAACA GTAATAGTCTGACAACAGTGAATTTTTTTTAAACATAACCGTTGTTTCTAGCAATGGGCCATGTGCTATA CAAATTATGGCTTATCAATATGATGTAAAACAGCTTAGCCATCAAAAGTCATTATTAGGCCAGGGAGTTG ATGTGACTGCTAAGCATACTTGCCTCATAAGCCCAGAGAGCTGAGTCAACCCCAGAACCCCAAATTAGGA CAGAAGAGAAAACCGACTCCACCAAGTTGTTCTGATTGCTGTATGCATGCTGTGGGATGTGTTCTTGAAC ATACAAAGTATGGGTTAAAATTTATTTTATTTTTAACTTCTTTGTGTCTCTGCATGTGTATGTGTACTAG TGTGCAGCTGACCACAGAATCCAGAAGAAGGGATAGGATCTCTGGGGTGGAGTTACAGATGGTTGTGAGT TGTACACTGTGGGTAATAGAAACCAAACTCGGGTCCTCTTAACCTCTCAGCCATCTCTCTGCAACCCCTC AAAAAAATTTTAAATGCCGCTTTTAGGATTCAGGGTTAGGGTTAAGTAGAGAGAGAGTACATGCATAGCC TGTGCAAAATCCTAGGTTCAACCCTAGCACCCCCAGACAACTGACAAGTATGGCATTTTTAGAGAAAATT AATTGTCATGAGAAACTGTCGTTGTTATTCTGTTAAGGAAAAAACAAATTACAAATGGCAACTTCTCCTC CCCTGTAGGGAAGATGTCCAGGTTCCTACACTCTCAGATGGTACCTACGTACCTATATGCCTTACTTAAG ATAAAGTCTAATGTGTAAACTAAACAGACTAAGGTATTAACCGCACATTTTATTATTTAAAGATATGTCA TCTTCCATTTTCAGTTTCCAGAAGAAATTTCTTATTTTGAAGAGGAAGAAAGAAAAGGAGTAGACAGATT TATGGGGGGGCCTCCTGCCTACATGAACAAGTAAGCAAGCTGAGGGTGAGAATCCCACTAAGTCCAGTGT AGGATGCATGCGTTGGGAGCATTTACTTACATGATTTCAGTTTTGTTGCAATTGGGGCTTGGGGCAAACT GTTTGAGGTCTTTGAGGGATTTGTAGTGGATCGTGCCTCGGCTGGTGCTGATGCAGGAGCATCGTGCATT CCTTATCACTAGGGTTCCTAGGCGGAAGATAATGCACATTACTTAGAATGACATCCTTTCGAATGAGGCT TGTCTCAGTCACTATTGCTGCGACTGAGTGTCTGTGACTGCTTTTCAAGGTAGGTGGTATTTCCATTCTG TATACCAGGAAGCCAAGGTTAAGGGCAAAGTCATTTAGCAAAAGGGAGCAGGGGAAGAACTTCATTTTAG GTCATTTAGAGTTCTTAACAACATCACTTCACAACCTTGGAAGAAGAGAAAACCTCCATCATACTTAAAT CCCTACTGTTAGACACACATATATGCAACCTGTCAGTACTACGGGGACCGGTTTCTTAAGCAGTTCCTCA ATCTACTTCTTGCTACTGGGGTCATGGAGTTGATGTAGGTGTGGACAGAAAGCTCTACTTACAACAATTG ATTACCATTTCAATCCTATTCCCTCTCATCCTCACTGCTCTGAAAAGTCTCTTTAAACCTATGTCTCCAT CCCTTATTGCTTACCCAGGTCAGTGTCTATTTACACAGGTTTTGCCAACACACAGTAACTATGGCACACC TACTTTACACACGTGGAAAACGCTCTCTAGGAAGGTTTTTTTTTTTTGTCCGAAGTAAGCAAAGTCATGA ATAAGGAGGTCAAAGGCAATCTCTCTGACTTCTGAATTCTCAGTTGGTTAACCACAGACATTCTTATCAG TGCACAGCATCGGTTGAGGAAAGTGATGCAGGAGACAACTTAGAAAAGGATCTCTTATGTCACTCTCTGT TGCCAGGGCTGCGGATGAAATATTTCACAGGATAAATTCGAAGTAAAGCACCCAGAATCCCCTTTACATC TGAGAAATGTTTTTTCATTATTAAAACATTTAAATATGCTTTGAACCCTCATTGGGAACTCACAATACCT ACGCCAAGAAGGCATATTTAACTTGGCAGGTATAGATTACGACCTTTGGATAACTGCTCACAGCATCTAA CTGCTTTGATGACCAAACAGTATTACTTTTTATTGCATGCAAGACATTCAGCTTTATCTGCTGGCCTGAG AAGGCTTTTACGGTGATTCAGTCCTCAGAACTTACTAACGTAAAACTCCTCACCTCGAACTCCACACTGC TCCAGGAAGATGATGCCCAAGAGGAAAAGAACAGCGGACTTCATGGCAGAGCTGAGTTCTATTGAGTCAC TGTGTTGGAGTGAAGTCCGAGAATGTCTT >gi|110626107|ref|NP_032625.2| C-X-C motif chemokine 9 precursor [Mus musculus] MKSAVLFLLGIIFLEQCGVRGTLVIRNARCSCISTSRGTIHYKSLKDLKQFAPSPNCNKTEIIATLKNGD QTCLDPDSANVKKLMKEWEKKISQKKKQKRGKKHQKNMKNRKPKTPQSRRRSRKTT Vaximmutor cysQ Mycobacterium tuberculosis H37Rv 888142 15609268 Rv2131c AL123456 NP_216647 83332 2392516 2393319 - 3'(2'),5'-bisphosphate nucleotidase CysQ 4.65 26760.05 267 >NC_000962.3:2392516-2393319 Mycobacterium tuberculosis H37Rv, complete genome ATCAGCGCCACGCGTCGGCGATGGCACCGAGCAGGATCGGCGCCACCTCGGCTCGACACATCAGCAGATC CGGCAGGTAGGGGTCCAGTTGGTTGTATCGCAGCGGCGAGCCATCGAGTCGTGACGCGTGCATGCCGGCG GCCAACATCACCCCAGCCGGCGCCGCGGAATCCCACTCCCATTGGCCTCCGGCGTGCAGGTAGGCGTCGA CGTAGCCGTCAATGACGGCCATCGCTTTGGCGCCCGCCGAACCGATCGACACCGGTTGGATCGCCAGCGT CTGGCGGATGCGGTGCAGGACTGCCGGTGGCCGGGTGGCGCTGACGGCAATCCGCAAGGTGCCAGGAACG CCGGCCGGCGCGGCGCCGGAAGTCACCGTATCGGTGCGGTACACCACGTTGCCACGGGCCGGCAACGCCA CCGCGGCGTCGGTGATCTCGGGCTGGCCATTGGAGGAACGCCGCCACAGCGCAATGTGTACCGCCCAGTC GTCGCGACCCGGTGTGGAGAACTCGCGGGTGCCATCCAACGGGTCAATAATCCACACCCGATCGGATTTC AGCCGGGCCAGATCGTCGTGGGCCTCCTCACTGAGCACTGCGTCACCCGGCCGTTCGGCCTGCAGCCGTC GCAACAGCAGCGAGTTAGCCTGGCGGTCACCGGCTTCCCCGAGCGTCCATGGCTGATCGAAACCGATCTC CGCACGCACCTGGAGCAACAGCTTTCCCGCGTCCGCCGCCAGGTCGGCGGCCAGCTCGGCGTCAGTCAGG TCGTCCGTCAGATCAGGTGCGGCAGGGCTCACCA >NP_216647.1 3'-phosphoadenosine 5'-phosphate phosphatase [Mycobacterium tuberculosis H37Rv] MVSPAAPDLTDDLTDAELAADLAADAGKLLLQVRAEIGFDQPWTLGEAGDRQANSLLLRRLQAERPGDAV LSEEAHDDLARLKSDRVWIIDPLDGTREFSTPGRDDWAVHIALWRRSSNGQPEITDAAVALPARGNVVYR TDTVTSGAAPAGVPGTLRIAVSATRPPAVLHRIRQTLAIQPVSIGSAGAKAMAVIDGYVDAYLHAGGQWE WDSAAPAGVMLAAGMHASRLDGSPLRYNQLDPYLPDLLMCRAEVAPILLGAIADAWR Protective antigen Defb3 Mus musculus 27358 7304997 AC113099 NP_038784 10090 8 19293360 19295338 + defensin beta 3 11.07 6827.63 63 Also known as BD-3; MGC129397 >gi|372099102:19293360-19295338 Mus musculus strain C57BL/6J chromosome 8, GRCm38 C57BL/6J CCAGGCTTCAGTCATGAGGATCCATTACCTTCTGTTTGCATTTCTCCTGGTGCTGCTGTCTCCACCTGCA GGTGAGTCAGGGGAGTAGGGTCATCCCACACTGATAGGAGAGACAACACCCTACATCAGGTGTGTGTGTA TGTGTGTGTGTCTGTGTGCCTATGTATCTCTGTGTTTGTCTCGGAGTCTCCCTCTCTATGTATCTTTCTG TTTCTCTGTGTTTGCATCTCTCTCTGTGTCTTTGTGTTTCTGTGTCTCTATGTTTGTGTCTGTCTGTCTG TCTATCTCTCTCTCTTTCTGTGTATGTGTGCATGTGTATACCTTTATGTCCCTTAATCTTCGTGTGTTGG TTTGTCTCCCTCTCATTTTATGTGTGTGTGTTCCTCTGCGCATGCATCTGTCTTTCTGTCTCTATGTGTA TATGTCTGTCTTTCTGTGTATAAGTCCATATCTATGTTTCTGTCTGTGTCTTTTTCTCCTTTTTACATAT ACCCTCCTTACTTTTGCATTTTTCTTTCTTTCTTCTAAGCATTTTAGAAAATGTGTATTGTTGACTACTA ACACAAACCAAGGACTCTTTCTTTTTCCCTTGAAGGAGTCTAAGGTAGACACATGCTCTGCTCATCTGCA GCAATTGACTTCCATTTCCTAACTCCTGATGCTAAGCTTAAGATTATCAAATGAGTGTCTTATAGGTAGC TTAAAAATAGTAACATGAAGAATGTTTGATTCTTACATGAACAGAAAGGTATTAATTTAGAAATCTTTAC ATATAAGGAGTAGAGTCTCTGCTTACTCTAGAGAACTCCTCAGAATAACCAGCCTAGCCTGTTTGAGAAC TTCTGCTCCCCTTAGCCCCATGGAATACCCCTGAGCACACAGTGTCTACAGGGTTAATGAGGTTCATAGC ACAGGGGAGGCTATTAAATCCTGTCTCCCAAACTTTTGCTTAAGTGTATTTAGTGGAAAAAATTTATTTT CTGTAAATTATGTGTATATGTATTGATTTGTATATGGGTATGTGCCAAGTAGTACAGTACTCACAGAGGT CATAAAAGGAAATCATATCTCTCAGAGATGGAGTTATTTATAGGCACATATGGCCTGCCTGATATGGTAC TAGGAACTTATCTCAGGTCTTCAGCAGGAGCAGTATGCACTCCTACAGGCTGAGCCATCTTTCCAGGCTC CAAGCTGTGTTTCATACTTGACTTTAGACAAACAGAATAATGGGGTTAGACAACTCATGTGAGGGAGCTA AAACAAGTAATGAGTTGGAGAGGAATTGATGAACACATCTTAGGCCAACCTGTGGCCTCTATATGTGTGT GCACACATGCACATTCATGTGCACAGTCACACTCAGTGTCTGTTTCACATGCTAGAAGTAGGGGCTTGTG TGTGGAGAAGCAAAGGCAGGGCTGCTAAGAAGTAGCTAGGGAGTCACACAAAATCTTTCCTGAGCTACTG CATGCTGGCTTTGAATACTCCAATCAGTCAGGGGTTGAGCAGATCCTTCAACATTTGGGTCCTAGGAATA CCAGCACCTCCTCTACACTGCAACTGTGCCTGTCTCGAACAAGAATTGGATTCAGCCCTGCTAAAACAGA CATTACTGTTTGGGCAGAACAATCTGCTGGGATCTACTTATCATAAGAACACATTGGTATGTATTTCTGA CCTACCACTTTCAATTTTAATGTATGGTTTTTCTTACTTATCTTTCAGCTTTTAGCAAAAAAATCAACAA TCCAGTAAGTTGTTTGAGGAAAGGAGGCAGATGCTGGAATCGGTGCATTGGCAACACTCGTCAGATTGGC AGTTGTGGAGTTCCTTTCCTCAAATGCTGCAAGAGAAAATAGAGAAGACCAAGATCCCGTTAAACAAAGA AAACACCAGAATTTGCTCCTCCATGAAGATGGACAAATCTTAAACCAAATTAATATTCCTTGATTAAATG CAAACAAGTGCTCCCTAGC >gi|7304997|ref|NP_038784.1| beta-defensin 3 precursor [Mus musculus] MRIHYLLFAFLLVLLSPPAAFSKKINNPVSCLRKGGRCWNRCIGNTRQIGSCGVPFLKCCKRK Vaximmutor DEFB5 Bos taurus 783935 195539557 AJ278799 NP_001124233 9913 27 ? defensin, beta 5 10.51 6632.32 64 Also known as BNDB-5 >NP_001124233.1 beta-defensin 5 precursor [Bos taurus] MRLHHLLLVLLFLVLSAGSGFTQVVRNPQSCRWNMGVCIPISCPGNMRQIGTCFGPRVPCCRRW Protective antigen DnaK Mycobacterium tuberculosis H37Rv VO_0012408 885946 15607491 Rv0350 AL123456 NP_214864 83332 419834 421711 + chaperone protein DnaK 4.59 61176.37 625 heat shock protein 70; assists in folding of nascent polypeptide chains; refolding of misfolded proteins; utilizes ATPase activity to help fold; co-chaperones are DnaJ and GrpE; multiple copies in some bacteria >NC_000962.3:419834-421711 Mycobacterium tuberculosis H37Rv, complete genome CATGGCTCGTGCGGTCGGGATCGACCTCGGGACCACCAACTCCGTCGTCTCGGTTCTGGAAGGTGGCGAC CCGGTCGTCGTCGCCAACTCCGAGGGCTCCAGGACCACCCCGTCAATTGTCGCGTTCGCCCGCAACGGTG AGGTGCTGGTCGGCCAGCCCGCCAAGAACCAGGCAGTGACCAACGTCGATCGCACCGTGCGCTCGGTCAA GCGACACATGGGCAGCGACTGGTCCATAGAGATTGACGGCAAGAAATACACCGCGCCGGAGATCAGCGCC CGCATTCTGATGAAGCTGAAGCGCGACGCCGAGGCCTACCTCGGTGAGGACATTACCGACGCGGTTATCA CGACGCCCGCCTACTTCAATGACGCCCAGCGTCAGGCCACCAAGGACGCCGGCCAGATCGCCGGCCTCAA CGTGCTGCGGATCGTCAACGAGCCGACCGCGGCCGCGCTGGCCTACGGCCTCGACAAGGGCGAGAAGGAG CAGCGAATCCTGGTCTTCGACTTGGGTGGTGGCACTTTCGACGTTTCCCTGCTGGAGATCGGCGAGGGTG TGGTTGAGGTCCGTGCCACTTCGGGTGACAACCACCTCGGCGGCGACGACTGGGACCAGCGGGTCGTCGA TTGGCTGGTGGACAAGTTCAAGGGCACCAGCGGCATCGATCTGACCAAGGACAAGATGGCGATGCAGCGG CTGCGGGAAGCCGCCGAGAAGGCAAAGATCGAGCTGAGTTCGAGTCAGTCCACCTCGATCAACCTGCCCT ACATCACCGTCGACGCCGACAAGAACCCGTTGTTCTTAGACGAGCAGCTGACCCGCGCGGAGTTCCAACG GATCACTCAGGACCTGCTGGACCGCACTCGCAAGCCGTTCCAGTCGGTGATCGCTGACACCGGCATTTCG GTGTCGGAGATCGATCACGTTGTGCTCGTGGGTGGTTCGACCCGGATGCCCGCGGTGACCGATCTGGTCA AGGAACTCACCGGCGGCAAGGAACCCAACAAGGGCGTCAACCCCGATGAGGTTGTCGCGGTGGGAGCCGC TCTGCAGGCCGGCGTCCTCAAGGGCGAGGTGAAAGACGTTCTGCTGCTTGATGTTACCCCGCTGAGCCTG GGTATCGAGACCAAGGGCGGGGTGATGACCAGGCTCATCGAGCGCAACACCACGATCCCCACCAAGCGGT CGGAGACTTTCACCACCGCCGACGACAACCAACCGTCGGTGCAGATCCAGGTCTATCAGGGGGAGCGTGA GATCGCCGCGCACAACAAGTTGCTCGGGTCCTTCGAGCTGACCGGCATCCCGCCGGCGCCGCGGGGGATT CCGCAGATCGAGGTCACTTTCGACATCGACGCCAACGGCATTGTGCACGTCACCGCCAAGGACAAGGGCA CCGGCAAGGAGAACACGATCCGAATCCAGGAAGGCTCGGGCCTGTCCAAGGAAGACATTGACCGCATGAT CAAGGACGCCGAAGCGCACGCCGAGGAGGATCGCAAGCGTCGCGAGGAGGCCGATGTTCGTAATCAAGCC GAGACATTGGTCTACCAGACGGAGAAGTTCGTCAAAGAACAGCGTGAGGCCGAGGGTGGTTCGAAGGTAC CTGAAGACACGCTGAACAAGGTTGATGCCGCGGTGGCGGAAGCGAAGGCGGCACTTGGCGGATCGGATAT TTCGGCCATCAAGTCGGCGATGGAGAAGCTGGGCCAGGAGTCGCAGGCTCTGGGGCAAGCGATCTACGAA GCAGCTCAGGCTGCGTCACAGGCCACTGGCGCTGCCCACCCCGGCGGCGAGCCGGGCGGTGCCCACCCCG GCTCGGCTGATGACGTTGTGGACGCGGAGGTGGTCGACGACGGCCGGGAGGCCAAGTG >NP_214864.1 chaperone protein DnaK [Mycobacterium tuberculosis H37Rv] MARAVGIDLGTTNSVVSVLEGGDPVVVANSEGSRTTPSIVAFARNGEVLVGQPAKNQAVTNVDRTVRSVK RHMGSDWSIEIDGKKYTAPEISARILMKLKRDAEAYLGEDITDAVITTPAYFNDAQRQATKDAGQIAGLN VLRIVNEPTAAALAYGLDKGEKEQRILVFDLGGGTFDVSLLEIGEGVVEVRATSGDNHLGGDDWDQRVVD WLVDKFKGTSGIDLTKDKMAMQRLREAAEKAKIELSSSQSTSINLPYITVDADKNPLFLDEQLTRAEFQR ITQDLLDRTRKPFQSVIADTGISVSEIDHVVLVGGSTRMPAVTDLVKELTGGKEPNKGVNPDEVVAVGAA LQAGVLKGEVKDVLLLDVTPLSLGIETKGGVMTRLIERNTTIPTKRSETFTTADDNQPSVQIQVYQGERE IAAHNKLLGSFELTGIPPAPRGIPQIEVTFDIDANGIVHVTAKDKGTGKENTIRIQEGSGLSKEDIDRMI KDAEAHAEEDRKRREEADVRNQAETLVYQTEKFVKEQREAEGGSKVPEDTLNKVDAAVAEAKAALGGSDI SAIKSAMEKLGQESQALGQAIYEAAQAASQATGAAHPGGEPGGAHPGSADDVVDAEVVDDGREAK Protective antigen In an ex vivo transfection approach with a retroviral vector a single antigen (hsp70) could evoke strong protection in mice [Ref1533:Lowrie et al., 1997]. drrC Mycobacterium tuberculosis H37Rv 888491 15610075 Rv2938 BX842581 NP_217454 83332 3274071 3274901 ? daunorubicin-DIM-transport integral membrane protein ABC transporter DrrC 10.46 26320.25 276 Rv2938, (MTCY19H9.06), len: 276 aa. Probable drrC, daunorubicin-DIM-transport integral membrane protein ABC transporter, probably involved in daunorubicin resistance and phthiocerol dimycocerosate transport (see citations below), equivalent to Q9CB71|ML2350 PROBABLE ANTIBIOTIC RESISTANCE MEMBRANE PROTEIN from Mycobacterium leprae (276 aa), FASTA scores: opt: 1434, E(): 1.2e-81, (79.0% identity in 276 aa overlap); and Q49941|DRRC|L518_F3_76 PUTATIVE DAUNORUBICIN RESISTANCE TRANSMEMBRANE PROTEIN from Mycobacterium leprae (244 aa), FASTA scores: opt: 1194, E(): 8.3e-67, (76.85% identity in 242 aa overlap). Also similar to others e.g. Q9XCF9 DRRC PROTEIN from Mycobacterium avium (263 aa), FASTA scores: opt: 538, E(): 3.7e-26, (32.65% identity in 251 aa overlap); Q9S6H3 DAUNORUBICIN RESISTANCE PROTEIN C from Mycobacterium avium (263 aa), FASTA scores: opt: 533, E(): 7.6e-26, (32.25% identity in 251 aa overlap); P32011|DRRB_STRPE DAUNORUBICIN RESISTANCE TRANSMEMBRANE PROTEIN from Streptomyces peucetius (283 aa), FASTA scores: opt: 276, E(): 6.6e-10, (21.07% identity in 261 aa overlap); etc. Note that Rv2938|drrC belongs to the transcriptional unit Rv2930|fadD26-Rv2939|papA5 (proven experimentaly). >gi|57116681:3274071-3274901 Mycobacterium tuberculosis H37Rv, complete genome CATGATCACGACGACAAGTCAGGAAATCGAGCTTGCACCCACACGTTTGCCAGGCTCGCAAAACGCTGCT CGGCTGTTCGTTGCGCAGACCCTTTTGCAGACCAACCGGTTGCTAACTCGATGGGCACGTGACTATATCA CCGTTATCGGAGCGATCGTGTTACCGATTCTCTTCATGGTGGTGTTGAACATTGTGCTAGGTAACCTAGC TTATGTCGTAACCCACGACAGCGGGCTCTACAGCATTGTTCCGCTGATCGCACTCGGCGCCGCGATCACT GGGTCAACTTTTGTCGCGATCGACCTGATGCGCGAGCGCTCCTTCGGACTGCTTGCCCGACTGTGGGTGC TGCCCGTGCACCGAGCATCGGGCCTGATCTCTCGAATCCTGGCAAACGCGATTCGGACTCTGGTCACCAC TTTAGTGATGCTAGGTACTGGGGTGGTATTGGGTTTCCGGTTTCGACAAGGCCTGATCCCGAGCCTCATG TGGATTAGTGTCCCGGTGATACTGGGCATCGCAATCGCGGCTATGGTCACTACCGTCGCGCTTTACACAG CACAAACCGTTGTTGTCGAAGGCGTTGAGCTGGTGCAAGCAATCGCGATCTTCTTCTCCACGGGTTTGGT GCCGCTCAACTCGTATCCAGGCTGGATTCAGCCGTTCGTCGCCCATCAGCCGGTGAGCTACGCCATCGCG GCGATGCGCGGTTTTGCAATGGGTGGTCCGGTCCTCTCTCCGATGATCGGGATGCTGGTGTGGACCGCGG GTATCTGCGTCGTATGCGCCGTACCCTTGGCCATTGGCTACCGACGGGCCAGCACGCATTG >gi|15610075|ref|NP_217454.1| daunorubicin-DIM-transport integral membrane protein ABC transporter DrrC [Mycobacterium tuberculosis H37Rv] MITTTSQEIELAPTRLPGSQNAARLFVAQTLLQTNRLLTRWARDYITVIGAIVLPILFMVVLNIVLGNLA YVVTHDSGLYSIVPLIALGAAITGSTFVAIDLMRERSFGLLARLWVLPVHRASGLISRILANAIRTLVTT LVMLGTGVVLGFRFRQGLIPSLMWISVPVILGIAIAAMVTTVALYTAQTVVVEGVELVQAIAIFFSTGLV PLNSYPGWIQPFVAHQPVSYAIAAMRGFAMGGPVLSPMIGMLVWTAGICVVCAVPLAIGYRRASTH Virmugen A drrC mutant is highly attenuated in mice and induces significant protection from challenge with wild type M. tuberculosis [Ref1673:Pinto et al., 2004]. Ebi3 Mus musculus 50498 7657052 AC154235 NP_056581 10090 17 55952622 55957021 + Epstein-Barr virus induced gene 3 10.35 23864.43 228 Also known as EBI-3; IL-27; interleukin-27 subunit beta >gi|372099093:55952622-55957021 Mus musculus strain C57BL/6J chromosome 17, GRCm38 C57BL/6J TGACTTCTCCCTTTTGGGGCCCTCCTTCTACCTCAAGGCCTTTCCCCACACATACACACAGCAGCTGGTG ACAGAATCAGGGTTCCCGTCACCATCATCATCTCCTGCCCCATACACTGGACAACTGAGCCACACTGGGC AGGTCCTTCCCTGGGGCCAGGTTCCCTGTGTGAGTCCCCTGTCCTTCACCCTCTCTCTGATGGGTCACTA ACTCGGATCCAAGGAACAGAGCCACAGAGCATGTCCAAGCTGCTCTTCCTGTCACTTGCCCTCTGGGCCA GCCGCTCCCCTGGTTACACTGAAACAGGTAGGTGACCACAGGGGGACTGGGGCACCTGGGCGGAGGGAAC TAGTCCTTCCCTTTCAGTTTAAGGTCATATCTACCATCAGCTGGGAACTCACACCTATACCCGTTGGACC TGTTCTCCTTCTACTACAGGGATGGGGAAACTGAGGCATGAAGCCTCAAAACCTCATTTATCCAGGCAGA GGCACCCTTGAACTTAGACATTTGTGGAGCTAGACAGTTGACTCTCAGAAACCCGAAACCTGAAGCCTCA CCTTTGTGACTCAGCAGCTGTAAAAGAAAAGAAAACGAAAAAGAAATGTAGACAATACTGGGTATAGTGG GGCACACCTGCCATGAAGCTCTCAGGAGTTCAGGGTCATCCACAGTCACATAGGAGGAAGCCAAGTATCA AGAGATTCCAATGTCCTGATCCACTCAGGGTAGGAGGCAGCCTAGGCCCCTTGGGAACCTGCTTCTAAAG CAACAGAACAAGTGTGGGGTGTGGGTAGTCTAGGGAGTTCTTCAGAATCCACAGTTCAAGGCCAGCCTAG GCTATGTGAGACCCTCCAGAAGGAGGAAGAGGAGGAGGAAGAAGGAAGAGGAGGGAGATGGAGGAGAAGG AGAAAGGAGAGAAGGGAAATTAGAAAAAAAGAAGAAAAAAAAAAAAAAACCTCAGGCCAGGCAGTGGTGG CACACGCCTTTAATCCCAGCACTTAGGAGGCAGAGGCAGGTGGATTTCTGAGTTCCAGGCCAGCCTGGTC TACAGAGTGAATTCCAGGACAGTCAGGGCTACACAGAGAAACCCTGTCTCGAAAAACCAAAAAAAAAAAA AAACAAAAAAACAAAAAAACCTCAGTTTTCTCATCTAGAAATTGAGCTAAGAACCTTGGGGTTTGTAGTA AGTATAGGAGCTCATGCTTCCATGCATAACAGGTCCTACATGGCCTACGGAAGCTCCTGTTCCTTTGTCT CCTCCAGCTCTCGTGGCTCTAAGCCAGCCCAGAGTGCAATGCCATGCTTCTCGGTATCCCGTGGCCGTGG ACTGCTCCTGGACTCCTCTCCAGGCTCCCAACTCCACCAGATCCACGTCCTTCATTGCCACTTACAGGTC AGAAAATGGAAAGGGTTTAGGAGGAAGCCAAGTATCAAGAGATTCCAATGTCCTGATCCACTCAGGGTAG GAGGCAGCCTAGGCCCCTTGGGAACCTGCTTCTAAAGCAACAGAACAAGTGTGGGGTGTGGGTATTCTAG GGAGTTCTTCAGAATCCACAGTCCCCAAGTCCCCCTGAGCATATCACCCCCTGAGCGTGACCCACAGTCA GCTGTGGGTGAGCTGGGGCCTCACGGGCACCCAGGTTCCTTTCGAGATTCACCATGGTGCTTCCCATGCA GGCTCGGTGTGGCCACCCAGCAGCAGAGCCAGCCCTGCCTACAACGGAGCCCCCAGGCCTCCCGATGCAC CATCCCCGACGTGCACCTGTTCTCCACGGTGCCCTACATGCTAAATGTCACTGCAGTGCACCCAGGCGGC GCCAGCAGCAGCCTCCTAGCCTTTGTGGCTGAGCGAATCAGTGAGTGTCTGTCCTCCCTGCCCCTCTCCA CCTGCTCCATCCCGTGGCTCCTCCCTGCCGCCGCCCCCCTACCCCCACCCACCTCTTGCTTTGTCCCTGC AGCTTCCATCCTTTGGCTCTGCTCCCGCCTCACAGGCTTCCTGTCGATGCCCCGGGGCCTTTGCATTGCT TCCTTCCTTGGCTTTCCTGGGTCTCTGCTCCAATGTTGGCTCCTCAGTGAGGCCTCCCCAAAAGTATATC ATTAAACATAAGCAATAGTCACAGTTAAGAGAGGATGAAAGCCAAAGGTGGTGTACAAGTCTTTAATCCC AGAGGTAGGCAGATCTCTGAGAATTCAAGGCCAGCCTGATCTACAGATCATGTTCAATGCAAGCCAGGGT CACTCCTGAGCACCTGTCTCAGAAAAGAAAGCCAAGAACAAAAGCTGATGCAGGTCGGTGAAAGGCTACA CAGCTCTCGAGGGTTTAAACCTGAAGTCTCGACATGATCGCCCAACTCCAATCCTAAGGATCTGCTCAAC AGCAATAAAGCTGTTCCTCCCTACCAATCCTTGGCAACATCATTGCTGGCATCCAGAAGGCCAGTGCCCC GTGCGTTCCCATGGAGCGATGGGCACCCAGTGCTTATCTAGCGTTACTCAGCCCTAACAAAGAGAGAGTG GGGCTCTGGCACCAACAGCTGTGGCGGGACCTTGAGGACACTACGCTCAGTGAGCAACCCAGACTCAACC CACCCAGTGTGGGCTGCAGTACAGAGGAGTCTAGAGACAGGAAGCCTGAGTGCTGAGGACAGAGAAGGGT AGGAAGATGGCTTGTGTTGTCATGAAGGAGTGTTCCATAAGTAGGTGGAGTAATGAAAACGCTCTCTGCA GATGCACGGGATGTCTTCTAGCAGAGACTTGTGCTCTCAGAATTGTATCTCAGAAAAGCCCTTCAGCCTA CTGGAGACTTGCACTTTTCTTTGCCTGTGCAACCCTTGTGACTTCTATCAGCAGTGATGAGAGCGGTCCA GCAAATGGTTTTTCCTTGAGCAGATAGACGAATGAAGGAATAAATGAATGCCTGCATGACAGATATCAAG TGGATGAGTGAATGATTTAGTAATGAATGAATGCATAATGAGTGAATGAAGGCAGAAATGACATGAGTGC ATGCACACGAATGACTTAATGAATGAACGAATGAATGCATGAATGACTTAGTGAATGAGTGAATAAATGC ACAACTGACATAATGAATGCATAATGAATACATGAAGGCCATGAGTAAATGAATGAATGACATGAGTGAG TGCATGAACGAATGAATGACTCAATTAGGGAGTGAATGAATGAATTCACAAAGGATACAGTGAATGACCT AATGACTGGGAAATGGATCCCTGAACGAATGAGTGTATAAACGATAAGCGCAAATGAATGAGTTAATGAA TCCAGAAACAAATCTGCACTTGAATCAACCAATAAATAAATGTGTGAGTGAGATGGTGAGTAACTGAGTA GAGGAATGAATTAGTGAAGAATTTAGTGAATACGTGAACGAATGAAGTGTGAGTATGAATGAACGGCAGG ACGGAGCAAATCCATGAATAGCTGTCTGAGGGCGAAAGCGAACATGTGAATGAATGAGAGTCACAAACGA AGACTTAGTGTGTGAGGGATGAACCGGCAGAGCGGACGCTGACCCCGGTCCTGCTCCCACCAGTCAAGCC GGACCCTCCGGAAGGCGTGCGCCTGCGCACAGCGGGACAGCGCCTGCAGGTGCTCTGGCATCCCCCTGCT TCCTGGCCCTTCCCGGACATCTTCTCTCTCAAGTACCGACTCCGCTACCGGCGCCGAGGAGCCTCTCACT TCCGCCAGGTGAGCTGCGCGCGCAGGGGGGCGTGGTCTGGAAGAAGGAGGAGCACAGAGGTGGATGCTGA GTGAGACTGGGTCCCACTGGGTAATCTCCTGTCTCCGCCTATCGCCTCTGTTGGAGCGTAGAGATTGTTG GTGTGATTCAGCAATAGACTTTACCTGCTTGCTGGGAATTCGAACTCACTGCAAGCTCTTTACCCACCAA GCCAGCTCCCCAGCTCCCCAATGCATTGTGGTCCTTCCCACCTCCTCCTTCCAATGGCCTGACACCCCTT CTCCCCACAGGTGGGACCCATTGAAGCCACGACTTTCACCCTCAGGAACTCGAAACCCCATGCCAAGTAT TGCATCCAGGTGTCAGCTCAGGACCTCACAGATTATGGGAAACCAAGTGACTGGAGCCTCCCTGGGCAAG TAGAAAGTGCACCCCATAAGCCCTGAAGAGGGACTGGATCCTTGATATCAGACCCTGATGTCGTCTACTT GAGGGTGGCAGGGTGGCCTGTCCTGAGTCTGAATACTGACTTTCCATGTACTGGGCTGCTCCGAAGCACT GGATAATTCACTTGACTTCTTCAGACCTCAATTTCCAACCCTGTGGGATGATCTTTCTTCTTCTGCCGGT GCGGGGCTTTGTGAACTTTGTGATTTAATTAGAAAGACGGATTAAATAAGAATCATCATC >gi|7657052|ref|NP_056581.1| interleukin-27 subunit beta precursor [Mus musculus] MSKLLFLSLALWASRSPGYTETALVALSQPRVQCHASRYPVAVDCSWTPLQAPNSTRSTSFIATYRLGVA TQQQSQPCLQRSPQASRCTIPDVHLFSTVPYMLNVTAVHPGGASSSLLAFVAERIIKPDPPEGVRLRTAG QRLQVLWHPPASWPFPDIFSLKYRLRYRRRGASHFRQVGPIEATTFTLRNSKPHAKYCIQVSAQDLTDYG KPSDWSLPGQVESAPHKP Vaximmutor erm(37) Mycobacterium tuberculosis H37Rv 885632 15609125 Rv1988 AL123456 NP_216504 83332 2231679 2232218 + 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) 12.41 17423.86 179 This region is a possible MT-complex-specific genomic island (See Becq et al., 2007 PMID:17545187). >NC_000962.3:2231679-2232218 Mycobacterium tuberculosis H37Rv, complete genome CGTGTCCGCCCTCGGACGGTCGCGACGGGCATGGGGCTGGCACCGGCTCCATGACGAATGGGCAGCGCGG GTAGTCAGCGCGGCCGCAGTGCGGCCCGGTGAGCTCGTGTTTGACATCGGCGCCGGCGAAGGGGCACTGA CGGCGCATCTAGTGCGAGCGGGGGCGCGGGTGGTCGCCGTGGAGTTGCACCCGCGACGAGTCGGTGTCCT CCGCGAGCGATTCCCTGGCATTACCGTGGTGCACGCGGACGCCGCCTCGATCCGGTTGCCCGGCCGGCCG TTCCGGGTTGTGGCGAACCCGCCGTACGGGATTTCGTCCCGCCTGCTGCGGACGCTGCTGGCACCCAACA GCGGGCTTGTCGCGGCCGATCTCGTGCTGCAGCGAGCCCTCGTATGTAAATTCGCTTCTCGCAACGCGCG AAGGTTCACCCTGACCGTCGGCCTCATGCTGCCACGGCGCGCGTTCCTGCCACCGCCGCATGTGGATTCC GCGGTGCTCGTCGTCCGCCGCCGGAAGTGCGGTGACTGGCAGGGGCGGTA >NP_216504.1 23S rRNA methyltransferase [Mycobacterium tuberculosis H37Rv] MSALGRSRRAWGWHRLHDEWAARVVSAAAVRPGELVFDIGAGEGALTAHLVRAGARVVAVELHPRRVGVL RERFPGITVVHADAASIRLPGRPFRVVANPPYGISSRLLRTLLAPNSGLVAADLVLQRALVCKFASRNAR RFTLTVGLMLPRRAFLPPPHVDSAVLVVRRRKCGDWQGR Protective antigen espA Escherichia coli O157:H7 str. Sakai 915474 15833810 ECs_4556 BA000007 NP_312583 386585 4593263 4593841 - T3SS translocator EspA 4.57 19184.78 192 >NC_002695.2:4593263-4593841 Escherichia coli O157:H7 str. Sakai DNA, complete genome GTTATTTACCAAGGGATATTGCTGAAATAGTTCTATATTGTAGAGATTGCACATCAGAACGTGCACTCGT TAAGAGATTTAATGTATTCGACATTTGCTGAATTTCGAGCTGGCTATTATTCACTACCGTTGTCAGGTTA TTCGCTTTAGCTGAAATAGCCGCCTTCACTGTTTGCAGATCACCAGCGCTTAAATCACCACTAAGATCAC GAATACCAGTTACACTTATGTCATTACGTGGATCGTTTATATAGTCAATCACGTCTTGAGGAAGTTTGGC TTTCGCATTCTTATCAGTGCTACTCTGAACATCAGCAATTTTGGCATCCACAAGATTAGCCATCTTTTGT GCCGTGGTTGACGCTTTAGATGCCTCATTCATATCAGCAAACTTTGCAATCGACAGATTACTTTGTGCCT GATACATATAAGAAAACATGAGAATCGCAGCCTGAAAAACACCGAGTTCCTCAAATAGCTTAACCACCTC ATCCTTCGACATATTACCTAAGTCATAGATCGTCGATGTCGAAGAACTCGCACTCACATTAACAACGGAT GTTGCATTTGATGTATCCA >NP_312583.1 T3SS translocator EspA [Escherichia coli O157:H7 str. Sakai] MDTSNATSVVNVSASSSTSTIYDLGNMSKDEVVKLFEELGVFQAAILMFSYMYQAQSNLSIAKFADMNEA SKASTTAQKMANLVDAKIADVQSSTDKNAKAKLPQDVIDYINDPRNDISVTGIRDLSGDLSAGDLQTVKA AISAKANNLTTVVNNSQLEIQQMSNTLNLLTSARSDVQSLQYRTISAISLGK Protective antigen espC Mycobacterium tuberculosis H37Rv 885770 15610751 Rv3615c AL123456 NP_218132 83332 4054811 4055122 - ESX-1 secretion-associated protein EspC 5 10100.3 103 Also known as snm9 >NC_000962.3:4054811-4055122 Mycobacterium tuberculosis H37Rv, complete genome GTCAGGTAAACAACCCGTCGATAGCCTTGCGCCACGCTTCGTCGGCCTCGCTATATATCTTCGCCGCAAT TCGAAGACTTTTGGCGAGATCGACACCGGCCGTATGCAAGGACGAGCCCAGGGCATTGTGGGCAGTCAAG TACACATTTAACGTGTCGTTGAACTGTGAGCAGTACGGACCGTGAGTGATCGCCACAGATTCGCCTAGGC CAGCGGCAGCTTCGACGCCCGAGGAGGCATCGACCGCCGCGTTGTCATGGTGCGACGCCAGTACACCGAG ACGCTCGGGCTGGACGGTCAAGTTTTCCGTCA >NP_218132.1 ESX-1 secretion-associated protein EspC [Mycobacterium tuberculosis H37Rv] MTENLTVQPERLGVLASHHDNAAVDASSGVEAAAGLGESVAITHGPYCSQFNDTLNVYLTAHNALGSSLH TAGVDLAKSLRIAAKIYSEADEAWRKAIDGLFT Protective antigen espI Mycobacterium tuberculosis H37Rv 886206 15611012 Rv3876 AL123456 NP_218393 83332 4353009 4355009 + ESX-1 secretion-associated protein EspI 10.28 66873.64 666 Also known as snm3 >NP_218393.1 ESX-1 secretion-associated protein EspI [Mycobacterium tuberculosis H37Rv] MAADYDKLFRPHEGMEAPDDMAAQPFFDPSASFPPAPASANLPKPNGQTPPPTSDDLSERFVSAPPPPPP PPPPPPPTPMPIAAGEPPSPEPAASKPPTPPMPIAGPEPAPPKPPTPPMPIAGPEPAPPKPPTPPMPIAG PAPTPTESQLAPPRPPTPQTPTGAPQQPESPAPHVPSHGPHQPRRTAPAPPWAKMPIGEPPPAPSRPSAS PAEPPTRPAPQHSRRARRGHRYRTDTERNVGKVATGPSIQARLRAEEASGAQLAPGTEPSPAPLGQPRSY LAPPTRPAPTEPPPSPSPQRNSGRRAERRVHPDLAAQHAAAQPDSITAATTGGRRRKRAAPDLDATQKSL RPAAKGPKVKKVKPQKPKATKPPKVVSQRGWRHWVHALTRINLGLSPDEKYELDLHARVRRNPRGSYQIA VVGLKGGAGKTTLTAALGSTLAQVRADRILALDADPGAGNLADRVGRQSGATIADVLAEKELSHYNDIRA HTSVNAVNLEVLPAPEYSSAQRALSDADWHFIADPASRFYNLVLADCGAGFFDPLTRGVLSTVSGVVVVA SVSIDGAQQASVALDWLRNNGYQDLASRACVVINHIMPGEPNVAVKDLVRHFEQQVQPGRVVVMPWDRHI AAGTEISLDLLDPIYKRKVLELAAALSDDFERAGRR Protective antigen EsxA (ESAT-6) Mycobacterium tuberculosis H37Rv VO_0010919 886209 57117165 Rv3875 AL123456 YP_178023 EMBL: AE000516 EMBL: AF004671 EMBL: AF420491 EMBL: BX842584 EMBL: X79562 GenomeReviews: AE000516_GR GenomeReviews: AL123456_GR InterPro: IPR010310 KEGG: mtc:MT3989 KEGG: mtu:Rv3875 PDB: 1WA8 Pfam: PF06013 PIR: A70803 TIGR: MT3989 TubercuList: Rv3875 UniProt: P0A564 83332 4352608 4352895 + ESAT-6 protein EsxA 4.19 9605.81 95 Rv3875, (MT3989, MTV027.10), len: 95 aa. esxA, early secretory antigenic target (see citations below), identical to Q57165|O84901|ESAT6 EARLY SECRETORY ANTIGENIC TARGET from Mycobacterium bovis (94 aa), FASTA scores: opt: 596, E(): 4.6e-33, (100.0% identity in 94 aa overlap). Also similar to Q50206|ESA6_MYCLE|ESAT6|ESX|L45|ML0049|MLCB628.12c 6 KDA EARLY SECRETORY ANTIGENIC TARGET HOMOLOG (ESAT-6-LIKE PROTEIN) (L-ESAT) from Mycobacterium leprae (95 aa), FASTA scores: opt: 236, E(): 3.3e-09, (36.25% identity in 91 aa overlap); and weak similarity with others proteins ESAT-like from Mycobacterium leprae. Also some similarity with O53266|ES69_MYCTU|Rv3019c|MT3104|MTV012.33c PUTATIVE SECRETED ESAT-6 LIKE PROTEIN 9 from Mycobacterium tuberculosis (96 aa), FASTA scores: opt: 131, E(): 0.03, (26.15% identity in 88 aa overlap); and other ESAT-like protein. Contains probable coiled-coil from 56 to 92 aa. BELONGS TO THE ESAT6 FAMILY. Note that previously known as esat-6.; esat-6 FUNCTION: Not known. Elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response (UniProt: P0A564) SUBCELLULAR LOCATION: Secreted protein (UniProt: P0A564) SIMILARITY: Belongs to the ESAT-6 (esx) family (UniProt: P0A564) >NC_000962.3:4352608-4352895 Mycobacterium tuberculosis H37Rv, complete genome CATGACAGAGCAGCAGTGGAATTTCGCGGGTATCGAGGCCGCGGCAAGCGCAATCCAGGGAAATGTCACG TCCATTCATTCCCTCCTTGACGAGGGGAAGCAGTCCCTGACCAAGCTCGCAGCGGCCTGGGGCGGTAGCG GTTCGGAGGCGTACCAGGGTGTCCAGCAAAAATGGGACGCCACGGCTACCGAGCTGAACAACGCGCTGCA GAACCTGGCGCGGACGATCAGCGAAGCCGGTCAGGCAATGGCTTCGACCGAAGGCAACGTCACTGGGATG TTCGCATA >YP_178023.1 ESAT-6 protein EsxA [Mycobacterium tuberculosis H37Rv] MTEQQWNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQ NLARTISEAGQAMASTEGNVTGMFA Protective antigen The heterologous prime-boost strategy of live Salmonella vaccine expressing Mycobacterium tuberculosis fusion antigen Age5B-ESAT6 (esxA) from the chromosome followed by a systemic boost of antigen and adjuvant reduced the levels of M. tuberculosis bacteria in the lungs and spleen to the same extent as BCG. Additionally, this vaccination regimen was observed to be statistically equivalent in terms of protection to immunisation with BCG [Ref1180:Hall et al., 2009]. In a prophylactic vaccine model, immunization with the Mycobacterium tuberculosis protein ESAT-6 with LANAC adjuvant elicited significant protective immunity against aerosol challenge with virulent M. tuberculosis [Ref1479:Zaks et al., 2006]. EsxB Mycobacterium tuberculosis H37Rv VO_0010951 886194 15611010 Rv3874 AF004671 NP_218391 3FAV EMBL: AE000516 EMBL: AF004671 EMBL: AF419854 EMBL: BX842584 GenomeReviews: AE000516_GR GenomeReviews: AL123456_GR InterPro: IPR010310 KEGG: mtc:MT3988 KEGG: mtu:Rv3874 Pfam: PF06013 PIR: H70802 SMR: P0A566 TIGR: MT3988 TubercuList: Rv3874 UniProt: P0A566 83332 4352273 4352575 + ESAT-6-like protein EsxB 4.31 10396.66 100 Rv3874, (MT3988, MTV027.09), len: 100 aa. esxB, 10 KDA culture filtrate antigen (see citations below, especially first), highly similar to O33084|CF10_MYCLE|ML0050|MLCB628.13c 10 KDA CULTURE FILTRATE ANTIGEN CFP10 HOMOLOG from Mycobacterium leprae (99 aa), FASTA scores: opt: 237, E(): 2.4e-08, (39.4% identity in 99 aa overlap). Also similar to O05440|ES6D_MYCTU|Rv3905c|MT4024|MTCY15F10.06 PUTATIVE ESAT-6 LIKE PROTEIN 13 from Mycobacterium tuberculosis (103 aa) FASTA scores: opt: 126, E(): 0.18, (23.1% identity in 91 aa overlap); and shows some similarity with other proteins from Mycobacterium tuberculosis. Contains probable coiled-coil from aa 49-93. BELONGS TO THE ESAT6 FAMILY. Note that previously known as lhp (alternate gene name: cfp10).; lhp, cfp10 SUBCELLULAR LOCATION: Secreted protein (UniProt: P0A566) SIMILARITY: Belongs to the ESAT-6 (esx) family (UniProt: P0A566) >NC_000962.3:4352273-4352575 Mycobacterium tuberculosis H37Rv, complete genome CATGGCAGAGATGAAGACCGATGCCGCTACCCTCGCGCAGGAGGCAGGTAATTTCGAGCGGATCTCCGGC GACCTGAAAACCCAGATCGACCAGGTGGAGTCGACGGCAGGTTCGTTGCAGGGCCAGTGGCGCGGCGCGG CGGGGACGGCCGCCCAGGCCGCGGTGGTGCGCTTCCAAGAAGCAGCCAATAAGCAGAAGCAGGAACTCGA CGAGATCTCGACGAATATTCGTCAGGCCGGCGTCCAATACTCGAGGGCCGACGAGGAGCAGCAGCAGGCG CTGTCCTCGCAAATGGGCTTCTG >NP_218391.1 ESAT-6-like protein EsxB [Mycobacterium tuberculosis H37Rv] MAEMKTDAATLAQEAGNFERISGDLKTQIDQVESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQKQELD EISTNIRQAGVQYSRADEEQQQALSSQMGF Protective antigen The 10-kDa culture filtrate protein (CFP-10, esxB) and 6-kDa early secretory antigen of T cells (ESAT-6) are secreted in abundance by Mycobacterium tuberculosis. Study demonstrated that protective immunity is induced by CFP-10 DNA vaccination in C3H mice as measured by a CFU reduction in the lung and spleen 4 and 8 weeks after challenge with M. tuberculosis [Ref1182:Wu et al., 2008]. esxC Mycobacterium tuberculosis H37Rv 886222 15611026 Rv3890c AL123456 NP_218407 83332 4373725 4374012 - ESAT-6 like protein EsxC 4.17 9522.78 95 Also known as ES6_11 >NC_000962.3:4373725-4374012 Mycobacterium tuberculosis H37Rv, complete genome TTTAGAACAAGCCCGCGATGGCCTGGTCGGTTCCGATCGCGTTGTCCAGCACGTGGCCGGTGGTAGTCCC ATGCTGACCCACCGTCTCAATGAGCCCCTGCAGCCCCGACAGCATCTGCGCCTGGGCGTCGAAAAACCCT TGCGCGCCGTGGCCCGCGAAAAACTCTTGCAGCGCATTTGTTTTGCTGGCGGTGTCTTCGTAAATCATGT GGAGCTGGCCGGCGCGCGAGCCCACGTCGGAAGCGAAGTCGGATACGGCTCCCGGGTTATACGTGATTTG ATCTGACA >NP_218407.1 ESAT-6 like protein EsxC [Mycobacterium tuberculosis H37Rv] MSDQITYNPGAVSDFASDVGSRAGQLHMIYEDTASKTNALQEFFAGHGAQGFFDAQAQMLSGLQGLIETV GQHGTTTGHVLDNAIGTDQAIAGLF Protective antigen esxD Mycobacterium tuberculosis H37Rv 886218 15611027 Rv3891c AL123456 NP_218408 83332 4374048 4374371 - ESAT-6 like protein EsxD 4.43 10598.02 107 >NC_000962.3:4374048-4374371 Mycobacterium tuberculosis H37Rv, complete genome GTCAGGATCCGTGGCTAGCGCCGAACAGCGCCTGAAACGCTGTCTGCGAGTCCGCCTCGTGTCCCTCCAT CAGGGCTGCGGCCTGCACGAGGCCCTCGGCCAGGCGCGTGCCCCCGGTAAGGACCTTGTTCAATTCATTG GTGATCTCGGTGGCTGTCATATGCGAAGCAACGACGCCGGTACCAGACCAGGTGGCGGGGTTCATGACGT TTTCCTGGTTGGCTAGGTAGCCCTTGGCGATTCCCATGGCTTGCTCCATATTCGCCTGGATATCGTTGGC GGTGCTGCGCAGCATCTGCGGTGTTACCTGAATTGTGTCTGCCA >NP_218408.1 ESAT-6 like protein EsxD [Mycobacterium tuberculosis H37Rv] MADTIQVTPQMLRSTANDIQANMEQAMGIAKGYLANQENVMNPATWSGTGVVASHMTATEITNELNKVLT GGTRLAEGLVQAAALMEGHEADSQTAFQALFGASHGS Protective antigen esxG Mycobacterium tuberculosis H37Rv 886604 15607428 Rv0287 AL123456 NP_214801 83332 351524 351817 + ESAT-6 like protein EsxG 6.51 9281.66 97 Also known as TB9.8 >NC_000962.3:351524-351817 Mycobacterium tuberculosis H37Rv, complete genome TATGAGCCTTTTGGATGCTCATATCCCACAGTTGGTGGCCTCCCAGTCGGCGTTTGCCGCCAAGGCGGGG CTGATGCGGCACACGATCGGTCAGGCCGAGCAGGCGGCGATGTCGGCTCAGGCGTTTCACCAGGGGGAGT CGTCGGCGGCGTTTCAGGCCGCCCATGCCCGGTTTGTGGCGGCGGCCGCCAAAGTCAACACCTTGTTGGA TGTCGCGCAGGCGAATCTGGGTGAGGCCGCCGGTACCTATGTGGCCGCCGATGCTGCGGCCGCGTCGACC TATACCGGGTTCTG >NP_214801.1 ESAT-6 like protein EsxG [Mycobacterium tuberculosis H37Rv] MSLLDAHIPQLVASQSAFAAKAGLMRHTIGQAEQAAMSAQAFHQGESSAAFQAAHARFVAAAAKVNTLLD VAQANLGEAAGTYVAADAAAASTYTGF Protective antigen esxH Mycobacterium tuberculosis H37Rv 886603 15607429 Rv0288 AL123456 NP_214802 83332 351847 352137 + ESAT-6-like protein EsxH 4.35 10191.72 96 Also known as cfp7; TB10.4A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004 PMID:14766927). Predicted possible vaccine candidate (See Zvi et al.,2008). >NC_000962.3:351847-352137 Mycobacterium tuberculosis H37Rv, complete genome GATGTCGCAAATCATGTACAACTACCCCGCGATGTTGGGTCACGCCGGGGATATGGCCGGATATGCCGGC ACGCTGCAGAGCTTGGGTGCCGAGATCGCCGTGGAGCAGGCCGCGTTGCAGAGTGCGTGGCAGGGCGATA CCGGGATCACGTATCAGGCGTGGCAGGCACAGTGGAACCAGGCCATGGAAGATTTGGTGCGGGCCTATCA TGCGATGTCCAGCACCCATGAAGCCAACACCATGGCGATGATGGCCCGCGACACGGCCGAAGCCGCCAAA TGGGGCGGCTA >NP_214802.1 ESAT-6-like protein EsxH [Mycobacterium tuberculosis H37Rv] MSQIMYNYPAMLGHAGDMAGYAGTLQSLGAEIAVEQAALQSAWQGDTGITYQAWQAQWNQAMEDLVRAYH AMSSTHEANTMAMMARDTAEAAKWGG Protective antigen This protein is used for generating a fusion protein for the vaccine AERAS-402 [Ref6774:Sivakumaran et al., 2021]. esxV Mycobacterium tuberculosis H37Rv NP_218136 CDD:310537 83332 ? ESAT-6 like protein EsxV 4.62 10489.63 170 Proteins of 100 residues with WXG; pfam06013 >NP_218136.1 ESAT-6 like protein EsxV [Mycobacterium tuberculosis H37Rv] MTINYQFGDVDAHGAMIRAQAGSLEAEHQAIISDVLTASDFWGGAGSAACQGFITQLGRNFQVIYEQANA HGQKVQAAGNNMAQTDSAVGSSWA Protective antigen ID93/GLA-SE protects against TB and MDR-TB in animals.[Ref5076:Bertholet et al., 2010] esxW Mycobacterium tuberculosis H37Rv NP_218137 CDD:310537 83332 ? ESAT-6 like protein EsxW 5.09 11866.28 174 Proteins of 100 residues with WXG; pfam06013 >NP_218137.1 ESAT-6 like protein EsxW [Mycobacterium tuberculosis H37Rv] MTSRFMTDPHAMRDMAGRFEVHAQTVEDEARRMWASAQNISGAGWSGMAEATSLDTMTQMNQAFRNIVNM LHGVRDGLVRDANNYEQQEQASQQILSS Protective antigen ID93/GLA-SE protects against TB and MDR-TB in animals.[Ref5076:Bertholet et al., 2010] fadD26 Mycobacterium tuberculosis H37Rv 887603 57117038 Rv2930 BX842581 NP_217446 83332 3243696 3245447 ? acyl-CoA synthetase 6.2 57587.87 583 activates fatty acids by binding to coenzyme A >gi|57116681:3243696-3245447 Mycobacterium tuberculosis H37Rv, complete genome GATGCCGGTGACCGACCGTTCAGTGCCCTCTTTGCTGCAAGAGAGGGCCGACCAGCAGCCTGACAGCACT GCATATACGTACATCGACTACGGATCCGACCCCAAGGGATTTGCTGACAGCTTGACTTGGTCGCAGGTCT ACAGTCGTGCATGCATCATTGCTGAAGAACTCAAGTTATGCGGGTTACCCGGAGATCGAGTGGCGGTTTT AGCGCCACAAGGACTGGAATATGTCCTTGCATTCCTGGGCGCACTTCAGGCTGGATTTATCGCGGTTCCG CTGTCAACTCCACAGTATGGCATTCACGATGACCGCGTTTCTGCGGTGTTGCAGGATTCCAAGCCGGTAG CCATTCTCACGACTTCGTCCGTGGTAGGCGATGTAACGAAATACGCAGCCAGCCACGACGGGCAGCCTGC CCCGGTCGTAGTTGAGGTTGATCTGCTTGATTTGGACTCGCCGCGACAGATGCCGGCTTTCTCTCGTCAG CACACCGGGGCGGCTTATCTCCAATACACGTCCGGATCGACGCGTACGCCGGCCGGAGTCATTGTGTCGC ACACGAATGTCATTGCCAATGTGACACAAAGTATGTACGGCTATTTCGGCGATCCCGCAAAGATTCCGAC CGGGACTGTGGTGTCGTGGCTGCCTTTGTATCACGATATGGGCCTGATTCTCGGAATTTGCGCACCGCTG GTGGCCCGACGCCGCGCGATGTTGATGAGCCCAATGTCATTTTTGCGCCGTCCGGCCCGCTGGATGCAAC TGCTTGCCACCAGCGGCCGGTGCTTTTCTGCGGCACCGAATTTCGCCTTCGAGCTGGCCGTGCGCAGAAC ATCTGACCAGGACATGGCGGGGCTCGACCTGCGCGACGTGGTCGGCATCGTCAGTGGCAGTGAGCGAATC CATGTGGCAACCGTGCGGCGGTTCATCGAGCGGTTCGCGCCGTACAATCTCAGCCCCACCGCGATACGGC CGTCGTACGGGCTCGCGGAAGCGACCTTATATGTGGCAGCTCCCGAAGCCGGCGCCGCGCCCAAGACGGT CCGTTTTGACTACGAGCAGCTGACCGCCGGGCAGGCTCGGCCCTGCGGAACCGATGGGTCGGTCGGCACC GAACTGATCAGCTACGGCTCCCCCGACCCATCGTCTGTGCGAATCGTCAACCCGGAGACCATGGTTGAGA ATCCGCCTGGAGTGGTCGGTGAGATCTGGGTGCATGGCGACCACGTGACTATGGGGTATTGGCAGAAGCC GAAGCAGACCGCGCAGGTCTTCGACGCCAAGCTGGTCGATCCCGCGCCGGCAGCCCCGGAGGGGCCGTGG CTGCGCACCGGCGACCTGGGCGTCATTTCCGATGGTGAGCTGTTCATCATGGGCCGCATCAAAGACCTGC TCATCGTGGACGGGCGCAACCACTACCCCGACGACATCGAGGCAACGATCCAGGAGATCACCGGTGGACG GGCCGCGGCGATCGCAGTGCCCGACGACATCACCGAACAACTGGTGGCGATCATCGAATTCAAGCGACGC GGTAGTACCGCCGAAGAGGTCATGCTCAAGCTCCGCTCGGTGAAGCGTGAGGTCACCTCCGCGATATCGA AGTCACACAGCCTGCGGGTGGCCGATCTCGTTCTGGTGTCACCTGGTTCGATTCCCATCACCACCAGCGG CAAGATCCGGCGGTCAGCCTGCGTCGAACGCTATCGCAGCGACGGCTTCAAGCGGCTGGACGTAGCCGTA TG >gi|57117038|ref|NP_217446.2| acyl-CoA synthetase [Mycobacterium tuberculosis H37Rv] MPVTDRSVPSLLQERADQQPDSTAYTYIDYGSDPKGFADSLTWSQVYSRACIIAEELKLCGLPGDRVAVL APQGLEYVLAFLGALQAGFIAVPLSTPQYGIHDDRVSAVLQDSKPVAILTTSSVVGDVTKYAASHDGQPA PVVVEVDLLDLDSPRQMPAFSRQHTGAAYLQYTSGSTRTPAGVIVSHTNVIANVTQSMYGYFGDPAKIPT GTVVSWLPLYHDMGLILGICAPLVARRRAMLMSPMSFLRRPARWMQLLATSGRCFSAAPNFAFELAVRRT SDQDMAGLDLRDVVGIVSGSERIHVATVRRFIERFAPYNLSPTAIRPSYGLAEATLYVAAPEAGAAPKTV RFDYEQLTAGQARPCGTDGSVGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKP KQTAQVFDAKLVDPAPAAPEGPWLRTGDLGVISDGELFIMGRIKDLLIVDGRNHYPDDIEATIQEITGGR AAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPITTSG KIRRSACVERYRSDGFKRLDVAV Virmugen An fadD26 mutant is attenuated in mice and induced significant protection from challenge with wild type M. tuberculosis [Ref1669:Infante et al., 2005]. FbpA (Ag85A) Mycobacterium tuberculosis H37Rv VO_0010952 886132 15610940 Rv3804c AL123456 NP_218321 1SFR EMBL: AE000516 EMBL: BX842584 EMBL: M27016 EMBL: U47335 GenomeReviews: AE000516_GR GenomeReviews: AL123456_GR InterPro: IPR000801 KEGG: mtc:MT3911 KEGG: mtu:Rv3804c PDB: 1SFR Pfam: PF00756 PIR: H70887 TIGR: MT3911 TubercuList: Rv3804c UniProt: P0A4V2 83332 4265641 4266657 - diacylglycerol acyltransferase/mycolyltransferase Ag85A 6.51 33304.71 338 Rv3804c, (MT3911, MTV026.09c), len: 338 aa. fbpA (alternate gene names: mpt44, 85A), precursor of the 85-A antigen (fibronectin-binding protein A) (mycolyl transferase 85A) (EC 2.3.1.-) (see citations below), identical to P17944|P17996|FBPA|MPT44 ANTIGEN 85-A PRECURSOR from Mycobacterium bovis (338 aa), FASTA scores: opt: 2341, E(): 1.2e-132, (100.0% identity in 338 aa overlap); and highly similar to other Mycobacterial antigen precursors e.g. O52956|A85A_MYCAV|FBPA ANTIGEN 85-A PRECURSOR (85A) from Mycobacterium avium (347 aa), FASTA scores: opt: 1987, E(): 1.7e-111, (82.55% identity in 338 aa overlap); Q05861|A85A_MYCLE|FBPA|ML0097 ANTIGEN 85-A PRECURSOR (85A) from Mycobacterium leprae (330 aa), FASTA scores: opt: 1936, E(): 1.9e-108, (83.0% identity in 329 aa overlap); O06052|A85A_MYCGO|FBPA ANTIGEN 85-A PRECURSOR (85A) from Mycobacterium gordonae (339 aa), FASTA scores: opt: 1932, E(): 3.3e-108, (80.45% identity in 338 aa overlap); etc. Also highly similar to P31952|A85B_MYCTU|FBPB|Rv1886c|MT1934|MTCY180.32 SECRETED ANTIGEN 85-B from Mycobacterium tuberculosis (325 aa), FASTA scores: opt: 1830, E(): 3.9e-102, (78.85% identity in 317 aa overlap); P31953|A85C_MYCTU|FBPC|MPT45|Rv0129c|MTCI5.03c|FBPC2 SECRETED ANTIGEN 85-C from Mycobacterium tuberculosis (340 aa), FASTA scores: opt: 1597, E(): 3.4e-88, (67.25% identity in 336 aa overlap).; mpt44; 85A FUNCTION: Proteins of the antigen 85 complex are responsible for the high affinity of mycobacteria to fibronectin. Possesses a mycolyltransferase activity required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity (UniProt: P0A4V2) SUBCELLULAR LOCATION: Secreted protein (UniProt: P0A4V2) PTM: Contains one disulfide bridge (UniProt: P0A4V2) SIMILARITY: Belongs to the mycobacterial A85 antigen family (UniProt: P0A4V2) >NC_000962.3:4265641-4266657 Mycobacterium tuberculosis H37Rv, complete genome GCTAGGCGCCCTGGGGCGCGGGCCCGGTGTTGGGCGTGGCACCCAGTGCCCGTTGCAGGTCGGGCTTCAT AGCGTTGAGCTGCGCGCCCCAGTACTCCCAGCTGTGCGTACCGCTGTCCGGGAAGTCGAACACGCCGTTG TGGCCGCCACCGGCGTTGTAGGCGTCTTGGAACTTGATGTTGCTGGTCCGCACGAAGCCCTCGAGGAACT TGGCCGGCAGGTTGTTGCCACCCAGATCCGACGGCTTGCCGTTGCCGCAGTACACCCAGACGCGGGTGTT GTTGGCGATCAGCTTCCCGACGTTCAACAGCGGGTCGTTGCGCTGCCACGCCGGGTCCTCCTTCGGGCCC CACATGTCGGAGGCCTTGTAGCCGCCAGCGTCACCCATCGCCAGGCCGATCAGGGTGGGACCCATCGCCT GGGAGGGGTCCAACAGGCCCGACATCGCTCCCGCGTAGACGAACTGCTGGGGGTGATAGATCGCCAGCGT CAGCGCCGAAGAAGCAGCCATCGAAAGACCGACGACGGCGCTTCCGGTGGGCTTGACGTGCCTGTTGGCC TGCAGCCACCCCGGCAGCTCGCTGGTCAGGAAGGTCTCCCACTTGTAAGTCTGGCAACCGGCCTTGCCGC AGGCGGGCTGGTACCAGTCGGAGTAGAAGCTTGACTGGCCACCCACCGGCATGACCACCGACAGGCCCGA CTGGTCGTACCACTCGAACGCCGGGGTGTTGATGTCCCAGCCGCTGAAGTCGTCCTGCGCGCGCAGGCCG TCGAGCAGGTACAGGGCGGGCGAGTTGGCACCACCACTTTGGAATTGGACCTTGATGTCACGGCCCATCG ACGGCGACGGCACCTGCAGGTACTCCACCGGCAAGCCCGGCCGGGAAAATGCCCCCGCGGTCGCCGTGCC ACCGACGGCGCCGACCAGACCCGACACTAGGGCCGCGCCGACGGCCCCGACCACGAGTCGACGCGACATA CCCGTGACGGCGCCACGAACCCTGTCAACAAGCTGCA >NP_218321.1 diacylglycerol acyltransferase/mycolyltransferase Ag85A [Mycobacterium tuberculosis H37Rv] MQLVDRVRGAVTGMSRRLVVGAVGAALVSGLVGAVGGTATAGAFSRPGLPVEYLQVPSPSMGRDIKVQFQ SGGANSPALYLLDGLRAQDDFSGWDINTPAFEWYDQSGLSVVMPVGGQSSFYSDWYQPACGKAGCQTYKW ETFLTSELPGWLQANRHVKPTGSAVVGLSMAASSALTLAIYHPQQFVYAGAMSGLLDPSQAMGPTLIGLA MGDAGGYKASDMWGPKEDPAWQRNDPLLNVGKLIANNTRVWVYCGNGKPSDLGGNNLPAKFLEGFVRTSN IKFQDAYNAGGGHNGVFDFPDSGTHSWEYWGAQLNAMKPDLQRALGATPNTGPAPQGA Protective antigen Researchers have developed DNA vaccines expressing M. tuberculosis antigen 85A (Ag85A, fbpA) and cytokine granulocyte macrophage colony stimulating factor (GM-CSF). The immune strategy of BCG-prime and DNA vaccine boost induced mice to generate efficient immune protection against M. tuberculosis challenge [Ref1181:Dou et al., 2010]. Fth1 Cavia porcellus 100379547 290491181 NP_001166318 10141 Un 9448211 9449938 + ferritin heavy chain 1 5.3 20567.49 182 >gi|347623380:9448211-9449938 Cavia porcellus strain inbred line 2N unplaced genomic scaffold, Cavpor3.0 supercont2_65, whole genome shotgun sequence CGCGGTCTCCTGCTTCAACAGTGCTTGGACGGAGCCCGGTGCTCGCCTGCCCTTACCGCCGCCCTCACCG CCGCCGCAGTCTAACCTCGCCCGCCGCCACCACCACCACCGCACCGCCGCCGCCGCCATGACGACCGCCT CGCCCTCGCAGGTGCGCCAGAACTACCACCAGGACGCCGAGGCCGCCATCAACCGCCAGATCAACCTGGA GCTCTACGCCTCCTACGTCTACCTGTCCATGGTGAGCGCGTGGCGTGCAGCCCGCAGTGGGCGCTCGGGG TTGCCTGGCGACAGCGCAAGCTCCCCAGGGACGGGAGTGCGCGCCTGGATGTAGGAGAGGCGGCGGCGCC ACTGACCCCCCTCGGCTGTCTCCCGCCGGGGCGTGCACTTTGAGGGTGCAGCTGGAGAGCTCGCGAACCT GTGTAGAAGATACCGTGCAGGGCGAACGCTCAACGCACTCCCTCTAGACAGGAGGATTTCCCTGTAAACA CTGCTGGAGGTGATTTTCGGTGCTAGTTTGAAGCTTCTTGAAGCAGCTCTCAGCGACCAAGTCCCAGTTT TTTGACACAAGATCTCACTGTGTAACCCAGGCTGCCCTTGAATGCACAGTTATCCTCTTGCCTCAGCCTC CTCAGTGCTGGGATTACATGCGTGCTCTATCAGTTTTCTAGTTTAGAGTTAAGAGCCTAGTCCCTTTCAG GAACACATCAGGCCCATTCAGCCCTGGCCCCTCTGGTTGTGTGGTGAGAAGGGCAAGCTGGACAGGCAGA GGACACTCACCACCTCCCCTCGCGACAGCCATCTCCCTTCCCATGGGAACCTTCACTGGACGGTGATGTT TATTTTCAGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTCGCCAAATACTTCCTTCACC AATCTCATGAGGAGAGGGAACATGCAGAGAAGCTGATGAAGCTGCAGAACCAGCGCGGTGGCCGGATCTT TCTTCAGGATATCAAGGTGAGTGGGGAAGGTCTGGATGTGAGAGACTCTAGGGGTCCTTCCCTCCCCTCC ATGTGCAGCTATGGCTGGGGCTCTCTGCCTCTGTGCGTGTCAGGGCCACTTTATGCTAATGTAGGCACTG AATCTGCAAGGACAGTGAAGCAGCAGCTCTAGAGGGGAGATGTGGGCATTAGACCCCAGCACTCACAGAC TGGGGCTGGCCCTGTTGGGGGGAGTCTGGGCACTGGTTGATCTGGTGCCTTTGCTCAGAAACCAGACCGT GATGACTGGGAGAATGGGCTGAATGCCATGGAGTGCGCCCTTCACTTGGAGAAGAGTGTGAATCAGTCAC TCCTGGAGCTGCACAAGCTGGCCACTGACAAGAATGACCCTCATGTGAGTACAGGGATTGGGCTGGCAGC AGAGGGAGGTGGGGTTCACGAGGCACCGGGAGCCAACCATCTACCTCTTGTCTCCTAGCTGTGCGACTTC ATCGAGACTCACTACCTGGATGAGCAGGTGAAGGCCATCAAAGAGCTGGGCGACCACGTGACCAACCTGC GCAAGATGGGTGCCCCTGAGTCTGGCATGGCCGAGTATCTCTTTGACAAGCACACCCTGGGGGACAGTGA CGAGAGCTAACGGTGCTTGGGCAGTGTGTGCATGCTGCGGCTACCTTTACCTTTTCTATAAATTGTACCA GAACATCTAAAGTTCTTTAATTCCTTCAAATAAAGAGATTTGGTACCC >gi|290491181|ref|NP_001166318.1| ferritin heavy chain [Cavia porcellus] MTTASPSQVRQNYHQDAEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKL MKLQNQRGGRIFLQDIKKPDRDDWENGLNAMECALHLEKSVNQSLLELHKLATDKNDPHLCDFIETHYLD EQVKAIKELGDHVTNLRKMGAPESGMAEYLFDKHTLGDSDES Vaximmutor Ftl Cavia porcellus 100379558 290491214 NP_001166329 10141 Un 8357696 8359000 - ferritin light chain 1 5.89 19110.14 175 >gi|347623385:8357696-8359000 Cavia porcellus strain inbred line 2N unplaced genomic scaffold, Cavpor3.0 supercont2_70, whole genome shotgun sequence TGCTGTGAAAGGCTTTATTTTTCTTTTGGTCCAAGATTTGGGAGGGGACTCCAAAGAGGTGAAAAAACTG CCTAGTGACTGGAGGGAGAGGGTCCAGCCAAAGCCCTGATGCCAGTGATGCTGGAGCCCCTCAGATACTG CCAGGCTCTGGGGCCTCCTAGTCGTGCTTAAGTGTGAGCCTTTCAAAGAGGTACTCGCCCAGCCCAGCCT GGGGACCATCCAGCCTGCGGAGGTTAGTGAGGTGGTCACCTATCTTCTTGATGAGCTTCACTTCCTCATC TAGGAAGTGGTTCTCCAGAAAATCACAAACCTGAAAAGCAAAAGAAGTCAGAAATCCAATTCTGGGCCAA GAGGGTGGAGCAAAGCCGTTTCTAACAATGGGACATGCAGGAGGCAGGCTCAGAAGTAAATGTGCCTACG GCTCCTGGGACAGTCACTTACATGAGGGTCTGTCTTGGCAGAGCCCAAGGCATGGAGATCCAGAAGAGCT TGGTTCAGGCTCTTCTCCAGGGTCAGAGCCGCTTCCATAGCATCAAGGGTCTTACCCCACTCATCCTCAG AAGGCTTCTACACAGAAAACAAAAGGCACAGAAACGGCCACGTTATCGTACACACTCACAGAGAGCTAAA ACTACAAATCTCAGTTTGAGTGATCCTTTCCTGCAGTAACTTGCACAGCCTACACCTGATTTCCCAGAAA TCCCAGCGACAACTGATCAGCTAAGGCCCAGGAGGAGCCCCATGGCAAGGACTGAAGTCGCTACTTCAGT CCTTGTTTACCTGCACGTCCTGGAAGAGCGCGCGGCCGCCGCGCTGGTTTTGCATTTTCAGGAGGCGCTC GGCACCCTCACGTTTCTCCTTGGCCAGCTCACGGAAGAAATGGCCCACGCCCGCCAAAGCCACATCGTCG CGGTCGAAATAGTAGCCCTGCGGGAAGAGATGGCGGGAAGTATGTGGAGAGTTGAGGCCAGGCAAGCGGC CTCCTCCCTCCGCCTGCCCCGCAAAACAAAGACGGCCTGCGCATGCGCACAGCCAACAAGTGCGCAGTTA CGAGGAAACAGGGCCTAGGGTGTTTGGGGGACCCTTACCAGAGAGAGGTAGGTGTAGGAGGCCTGCAAGT GCAGGTTCACCAAGCGGTTGACGGCAGCCTCCACCTCGGTGGAATAATTCTGACGAATCTGAGAGGTCAT TGCTGATCAGCGGGAAGGAGCTACCCACAAAAGAACGGTGTAGGCTGGCCCTGGAAGGAGCAGATGGTCG AGAAGATGGTCCCAAAATTTGTACCTGGAGAGTGGATGGGAGATT >gi|290491214|ref|NP_001166329.1| ferritin light chain 1 [Cavia porcellus] MTSQIRQNYSTEVEAAVNRLVNLHLQASYTYLSLGYYFDRDDVALAGVGHFFRELAKEKREGAERLLKTQ NQRGGRALFQDVQKPSEDEWGKTLDAMEAALTLEKSLNQALLDLHALGSAKTDSHVCDFLENHFLDEEVK LIKKIGDHLTNLRRLDGPQAGLGEYLFERLTLKHD Vaximmutor GPR182 Homo sapiens 11318 6005705 AC026120 NP_009195 9606 12 54427153 54444913 + G protein-coupled receptor 182 7.55 41850.38 404 Also known as AMR; 7TMR; ADMR; AM-R; G10D; gamrh; hrhAMR; MGC34399 >gi|157704453:54427153-54444913 Homo sapiens chromosome 12, alternate assembly HuRef, whole genome shotgun sequence CCAGCCTCCTCACAGCTCCCCATAGCCTGGACCTGCCGGCCCTCCCTCCAGGACCGAGGGGCTCCCAAGG GAAACTCAGGTGAGTGTCGCCCTCTCCCAGGGAAGATGGGAATCAGGGCTTGGAGACAACTGGCTATCCT GCATGATCCACAGGTTTTCCTAGGGATGAATCAGATTGTGTGGTGTGCGTGTGTGTGTGTGTGTTCCACA CTCACGGTCCAAATAACAAGACAGAAAAAGAGCAATAAAGATCTGTGCAAAAGGGCTGAGGAATGAAGTG CCAGGAGAGAGGTGGAGGTTGGAGACCACCTAGGAATGGTGGGTGTGGGGGGATTGAGGGGGTTGTTGAG GGGGAACTGGCTGGGGGTGGGGAGAAATGTTGGTGGACACCAACAGTCCTGGCCTCTTCCAGCTGAAACC CCTTAAGAAAGTATCTCTGCTCTTGGGTCAGAGCTAGCAAGTGAGTTGTGTATTATTTATTCACAGTTTG AAAGCTAAACTTCCCTTTCTTGCCTTAGCCCCAGAATCTTTCCCCAAAGCCCCCGACTCCCTCTGGCCTC TACCTTGAGGACACAAGGCTCTCGAGGTCTGCTTTCCCTTCCAACCCTCCCTTCATCCCAATAGGCGTGT GCTGGTCCCAATGTCAGTGAAACCCAGCTGGGGGCCTGGCCCCTCGGAGGGGGTCACCGCAGTGCCTACC AGTGACCTTGGAGAGATCCACAACTGGACCGAGCTGCTTGACCTCTTCAACCACACTTTGTCTGAGTGCC ACGTGGAGCTCAGCCAGAGCACCAAGCGCGTGGTCCTCTTTGCCCTCTACCTGGCCATGTTTGTGGTTGG GCTGGTGGAGAACCTCCTGGTGATATGCGTCAACTGGCGCGGCTCAGGCCGGGCAGGGCTGATGAACCTC TACATCCTCAACATGGCCATCGCGGACCTGGGCATTGTCCTGTCTCTGCCCGTGTGGATGCTGGAGGTCA CGCTGGACTACACCTGGCTCTGGGGCAGCTTCTCCTGCCGCTTCACTCACTACTTCTACTTTGTCAACAT GTATAGCAGCATCTTCTTCCTGGTGTGCCTCAGTGTCGACCGCTATGTCACCCTCACCAGCGCCTCCCCC TCCTGGCAGCGTTACCAGCACCGAGTGCGGCGGGCCATGTGTGCAGGCATCTGGGTCCTCTCGGCCATCA TCCCGCTGCCTGAGGTGGTCCACATCCAGCTGGTGGAGGGCCCTGAGCCCATGTGCCTCTTCATGGCACC TTTTGAAACGTACAGCACCTGGGCCCTGGCGGTGGCCCTGTCCACCACCATCCTGGGCTTCCTGCTGCCC TTCCCTCTCATCACAGTCTTCAATGTGCTGACAGCCTGCCGGCTGCGGCAGCCAGGACAACCCAAGAGCC GGCGCCACTGCCTGCTGCTGTGCGCCTACGTGGCCGTCTTTGTCATGTGCTGGCTGCCCTATCATGTGAC CCTGCTGCTGCTCACACTGCATGGGACCCACATCTCCCTCCACTGCCACCTGGTCCACCTGCTCTACTTC TTCTATGATGTCATTGACTGCTTCTCCATGCTGCACTGTGTCATCAACCCCATCCTTTACAACTTTCTCA GCCCACACTTCCGGGGCCGGCTCCTGAATGCTGTAGTCCATTACCTTCCTAAGGACCAGACCAAGGCGGG CACATGCGCCTCCTCTTCCTCCTGTTCCACCCAGCATTCCATCATCATCACCAAGGGTGATAGCCAGCCT GCTGCAGCAGCCCCCCACCCTGAGCCAAGCCTGAGCTTTCAGGCACACCATTTGCTTCCAAATACTTCCC CCATCTCTCCCACTCAGCCTCTTACACCCAGCTGAGGTAGAGGCCAGACTCCTCCAACAGTGAAGGAAAA GGCACAGGTGAGAGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGATAGCCATGGACTCTGGATCTTTA AAAATCTGCACTTCCAGCTGGGATTGGGAAGGGAGCTGAGGCTAAAGAGGTGCTCGGCAGAGGAAACTAG ACACATTCATTTCATAAATCACTCTGGCTGGAAACAGTGAACTCTGCAGGAACCTCACAAAACTCCTTTT GCGTTTGTGAGCTAGATGATCTTCCAGCTCACTATGCAGGTTATGGGGACAAGAGGCCAGAGGCCAAGGA ACCAGGCGGCCCCAGCAGGACGTTCCCTAGCTGTGCATCCTATGCACAGCCATGATAAGTGAAGGCGGAT GGGGATGGCAGGGGTGGGGGCGAAGCCAAGGTCTGAGACCTGCACCCTGCAGCTGGGTCAGCAGGATGGT ATCTCCACCCGCGGCCATCCAGGAAACACAGCTCCATTTCCTTGCTGACACCCTCCCCTCCCCGTTGTCC ATCCTGCTCCCTCCCCACAAGCAAGAGGAGTTGTCCCAGAGTCAGCTCCTCATAGCTGACCTCTTACCAG CTTCGGATCTAGGAAACTTCCAGACTTCTAGGGAGACACAGAGCTACCAGAAAGCCCAACCCACCCCCAT CTCTTTCTCTCCAGATCATAGGAAAGTATCAAGGGATGTCATTCCGGGAGCAGCCCTTGGGACAGCCAGC AGCAGGTGCTGGCAGATGCTCTGTCCTTCACCGTCTGTCCCCGGCACCAGATGGGGAAGGCCACAGTGGA AACTTCTCAATTCCGTCACAGGGCAGAGCTCTGACACTGTTATAAGTCAGTTCTCCCTGGCCATGTCTTT TCCTAATTTTCCTGTCCCACAACTTAGGTTCCTTAACACTTGTTCTACAGATGAAACAAAAAAGTCCTCT GTTAACAAGTAGACCTTCTGGAAAAGGATCACTTATGTTTGGGAGACTAGGACCCTGTCCATGAAGAACT CTGAAGTAAAGGCCTAAGAACAGGATCTAGTTTCCGCCTTCCAATTCCACGTCCTTTGCCTCTTAATCCT GCCCGTGGTAAGGGGACTGGATGGAATCACTGATAGCCTCTTCACCATCTCCTCTGTCCTGCTTAAGTGC TCCATGTGTCTGGAATGTTTAACTTGGAGTAACTACTCACTGACTTGGAGACCACCTGTTCTACTTTGAG AAATCAGAACTGTTCTGTGGATGCACTGACAGCGTGCTCCAACTGCATATGCACCCAGGTTTGTCAGGAG CATGGAAGGAAACCTGCATCTCCCTGTTTACTGGGCCCAAACCTGAAAGGGTCTTAAGCTGCAGACAGCT TCTCAGTAGCACAGAGCTGAAGGCTTTTTGAACCAGGAGTCTGGGTTGTGGCTTGGGTCGCAGTAAGGGC TTAGCCTATCAATGCAGAATTCACATTGAAAAAGACTTTCCTCTCTTGTCCCAACACAAAAAAGCAAGCC AAGATCTGATGCCCATATCACTGCCTCCTCTGACTTGGGCTACAGGAGTTTCCTGAAGGAAACCAAATTT TACATCTCTGTCACATTCCAGATCACCTTAGAGGCCACGCTCCCAAGATTAGTTATCACCCTGGCAGTAT GAATACTTCCCTAAGGCCTCCCATCCATGGAGGGGAAGAGTGGGAACCAGCTGTTACACTCAGCATCTAC TGAGCACTGATGGGAGCCCTGCCTGGGCCATGTGCTGTGGGGCCAAATGGACATCACTCCCTAACTTCTT GAGGTCTGTCTCCTCTGTGGATTCACGCATGTATCAACCTCATTAAACTATGCCAAACTTTTGCCTCCTC CCTGTTTCAATCTGCCTCGCTCCACTCAAAGACACACCATGTCCCCGCAAATCAAATGGCATTGGCAAGA CAAACTGTAGCCAAATCTCTCACTTAGTAACGTTAGTCCAACTAGACACGGGCAGGGACCCACCACTCTC TCTGGGCTGACGGAGGATCCTCTCAGGGATACCCCCTGAAAAGTTTGAGTCTTTGACTTAATTTCCCACT AAAAACAGGTCAACAAGTTAATCCTGGGGAATTCCAGCTACAGGAAAGGAAAATGTGCTACCTGAAAGAA TTCTGGCATCTGCTGGGCAGAAATGTGCTGCTGAAAGGGTGATGAGCCCAGATATTCTGAACTCCAGGTA TGAGCATATTCATAGTAGGTAGGAAGAGAACTCTCATTTCAAATTTCTTTTTTTATTCTAAATAAAAACC ACACTTTGTGCTGAACAATGGAATATAAAAGAATCAGATGTACAATGATTTTAGACATCTACTATTTGGG GAAAAAAGTTTACAAAATATACAAAACTTTACAGCAATAGATAGTAAACACTTAAATATTAGGAGGTCAG TACTTATTAATTTAATGGAAGGAGTTAGACGTCAACAACTCTTCTCTGTTTCATAAGAGACTTTAGCTAT TCAAGGTGGGGGCGGCGAGTGGATTGGACAGGGGAAGGGTTTTAAAGAAACATCAAAATCGAGTTTCATT TTCATTCTGATGTTAAGCTTATGATGCATAACCACATTGAAACTAATGCTGTGCACCCTGCCTCCTGACT CATTTGGTGCAGGATTTAGGGAAAAGAGTCCCAGATGGGTGCAGACCACCCCATAGAGCCCACTCCCATA CCAATAAATACAGTGTCTATCTAGAATTTGCTTTGGAAGTTAAGCAGTCTTGGGCTTGGGGAAAGGGGCA CAGAAGGAGGCCAGAGGAAGAAGAAAATTCAACACTAGCAAAAACCTGTCTAGGAAAAAGATATTTAAGC CCAAAGGTTTGGCCTGGAGACAAACAGGTACTCACAGATCAGAAAAGCAGAATATAAAAAAAAACTTTTA CCCATCTCCTGGTTCTTAGGGGTCACCTGGGCCTGACCCTTATCCCACCTCCTTTCTTCCTAACTAGCCA TGGACTACGGGAACTCCATGGAGTCATAAAATTTAGGACCAAATACTAAGGTTCAGCAGAGCTTCCTAGC GGGTGCTGTGGCATCAGTGAGTCACCCAGTGGGTCACTGGAGTGCCAGGATACTAATCTCTGCCTCTGGC CACATGGAGCCTCTGCTGTTTAACCCCGGGTGCCACACAAGTATTACCATTTTCCCCTCGGAAAACAAGT TGGGAATGGCCTCATATTTGGTTTAATGCTCTATGACCACCACCTTGAAATTTTTAATAATTTATGAATA AGAGGGCTCCACAAATGATGTAGCTGGTCCTGAGTACATACCAAGAAAATGGGAGAAGAGGATGTTCAAG GCTTAAACAGAACTTCAGAGTAACACAGAAGAGAGTAGTAAACTAGGTTCCAGGAGAGAGGGAGCAACAA AATCAAATGAACAGAAAGTCAAAACAATTAAAAATGCAAAATAAAATGAATACCTTCTACCAGGTATCCC AGGGTGCCCTCTGCCACAATATAGTGTTTCAAGCAAAACCATTCAGAAGAGGGTGAGCTGGAGGCAGAGG AAGCAGACATGACTTTGGTGGGGTGGGGGGAAACGGGAAGTTCAGGTGTGAATACAGGGGTCACCAACAG GGAGAGGGTTTGGTCAACAAATCTCATGAGCTACTGATCCTATGACGTATCTGACTGGGACCAACTGAGC CACCCTGATCAATCTGATCAGCCCCCGGGGGATCAGGGGATGCAGTTTTCTGTTTCGTGTTTCAAGGGCC AAGTTTCAAGGATCTGCTTTTTGGTTTACTGGCAAAAAGGGAAAACTTCACAGTTTCAGGTTAAGTACAC AGACCACCCAGCATCCATGATGCCCTCTGTTAAGGAAAAAGGTTTCTCCAGCACATTTATCTGCTCTCAG CCACTCGGCTGGTTCCTGTAATTACCACCTCTCTCACACCAGCATTTCAAAAATCAGTCCTCACCAACAG TCTGGGAGGGATTGCTCATGCTTGAAAATGTAGCAGACACTTGACTGTAGTCTGTTACACATTTCCTTCT TAAATTCTAATGGTCAGTGTGAGTAAGAAAGGGAGAAGCCAGTCAGCAATGAAGAGTCTCAACACTGCTT TCCTGATGAGGATGTCCTCAGAGGACACTGAACAAGGTGGGTTTAGATGGTCAAAGGTGGACCTTTAAAC TGGCCACTGTACAAGTCCCTTCATCTAATGGTCACCCCACCAGAGAGCCAGGCACTCCAACTCTGAGGCT TTATCAGTTCAGGGCTGGGGAGAAGCAGCAGGTCCAACAAGGATGGAGCTTTGGTTTGTCTGACATCTCA ATATAAATAAAGACAGGAGGCTTCCAAACTGTGCAAATAGGTCATTCATGTCACTTGTTCCCCTCAACCC TGTCCCCAATCTACTCCATCACAGGAAGGAAGGGGGATGAAAGGCCTGGAATTTAACTGTCCCGAACTTT ATAGAACATACACCCATGGCCTAAAGTGTTCCCTGGGTTTATTATGCTAGAGACAATCTGGAGAAGAAGA ATTTGCTTAAGGGCTTGACATGTCTTCCCTTAGTTATACCCCTGGTTCTCCACATCTCTGGTCAAGGCTG TTCTCTGGAATATGGCTACTTGCTGGGGAGAAACTCATTTTCCTAAAGTCTCTTATACAAAAGAGATGCT GGGTAATGTGTATACAAAAACAAAAATAAATAAATATTAATGTCCAAAAAGGCTTTGTGAGTTAATGAAA ACCACTTGCTTGTGAAACAGCCCGGGGTGTTGCTGAATCCCACCAGGGGCAGTGCCAGGGCTGAGGCCAG GGACTCCCAGAGCTCACCTGAGCAGGGTGAGGTAGTATGAGCTACTGAGGTGGCACCTCACAAGTATGCA AGATCGAGGGCCCTATCCCTCAAGACTTCTTTGGGCAAAGCCCCTTAGAAAACCAAGTAAGAGTGGAGCT AACCAGCTGCGTGGGCTGTGCCGGCACACTCTGCAGCCACCCACGGAGGGCCTGAACCAGCTGCCATCCA ATAGCGCCCCCTGGAGGTGATGGCAACGACAGATCAGGATAAATTCCAGCAGGTCAAAGGTGAGCTGACA AAGCTCCGGCACTGTTAGCACAAAGTAAACAGGTTACCCGACTAGGACAGACAGTCAGAACGCACACCCT GCTACACACCACCAGTTACTCAGGTCCCTTTAATCCTGAAGGGCTCGCGGTCTGCCACTCCACCACTTCA TGGAGTCAGCTCATCTACACCACGGGCCAAACACTTCTGGACGGGAGACCAGATATAAATTAAGGCAAGG CTGCTGCTGTCAGAGACATCCCCACCTCTCCACCAAGACACTCATGGCCAACAGAAGTGGCCAAAACAGA TGACTATATCAAAGTGGTTCCCTATCCTCTCTGCCCCACAGAAGGCCGGACGTCCCGGGCCATCACTCCA TAAAATCCTGTTTCTTTGGAAATGAGGAGAAAAGAGAAACCTCTCCTGTCCATCCAGGCTGCCACTTCTG GTGGCACTGCAGGACACCCCTTCTGGCTGGCAGGGGTTCATCCCAAAGCTTTCTGACCTAAATAGAAAGC TCTGTGCCTAGTATGGATCTAGCTGCCAAAAGATTTTCACTGTTCAATCAGCAGATTAGGGCTGTAGATA AGCAGACACCAACATCCCTGATGTCTTGTCCCTGCTGAGGGCTGGAACCTGAGGGGACTGCCCTGCTGAC CTGTCTTTTCTGAATGATCACATTCCCAAAATCTTTCTCATTGCTGGTCATCTCAGTTGGAGGCCTGCAG TAACACCCACAGTGAAAACCTCCCTTTACCTCCTCTGTTAAGTGAGGCTGTTGGTTGGATTCTTAGCAGC ATAAAACCTATCAGTAAACTAAAACTAGAGAGGCTGGGGAAGGGGGTCTAGTGGACACCAACTGCTTGAC TAGATCCCAAAACCAGCCTCAAAAGAATGAAAAACATAGCCCTGCATGGGCAAGAACAGGTATGGAGGTA AATGTGATAGGGCTGTTGCTTCTCAACAACAGTAACAGCTTATGTGCTATTTCTTCTTCTTTTCTTCTTT AAACACAACACAGAACAGAATTCCTACCAAGGCAAAATTATAACTTCAGCTGGGGAGGGACCAACACCTC TTAGATATCAGCATCCTGGCTGCTGCTTGGGAGCTCCCCGTGGCTCTGCCGGGACCCAGTCAACTGTCCG GGTTCTTATCCGCCTCTTTGGAATGGATGATGTACATGAAGGTCTGCTCGATGGTGAAGTCAGGGGGGAG GCTGCCTTTGTGCACACCAACATGTTTGCTCATGAGGTTAAGGGTGGAACTGTAGTGCCCACAGACTGTG CAGCGGTACATGAGGGCCCCCGAGTGTGTCTTTAGGTGGCACTTGATGGTCGAGCGGCCTCGAAAGAACT TGTGGCACACCTTACATTGGTATGGCTTCTCCCCCGTGTGGATCCGCCGGTGGTAGTTGAATTCGCTTGT GTGGGCAAATCTTTTGCCACAGACCTTGCAGCTATACTTGCTGTTCCCAGGTTGGCCCTGCAGCGCCAGC TCTTCACCCAGAAACTCCTCTGCTGGCAGCTTCCGCTTCTGGAGAGCTGGGTGCTGCAGCCCAAAACCAG GCCGTGCATCAAGGCCACTGTTGCATTTGTGCTGGCTGACGTGGTAGCGATAGGCAGCCTCTGACTTGAA GCTCTGGCTGCACAAGCGGCAGTGGAAGAGGGCGTCTTCCAGACTTTGGTGCACAGCCATGTGCTTCTCT AGAAGATGCCAGTCCACAAAGCTGTTCGCACAGACAGAACAGGAGAAGACTGAGATGCCGAGATGGGACA GCGTGTGCCACATGAGGCTGCAGAGGTTAAGGTGACGCTGGTCGCAGACACTGCAGGCCTGGCCCTTCAA GTTTAGATGGTCAAGGATGTGGCCCCGGACCACATGGAAATCTTTGGCCAATACTTTCCCACAGGCAGCG CATTTCAGCTCTGGGGAGGCTGCCCCTGAAAAGAGACCCAGCTTCCCTGGCAGGGGATCGTTGGGGTGGA CAATGATGTTGTTCTCAAATATTCCATCCCGTCGGTGAAGGGTCAGCTGCCACTGGGTAAAGAACTTAGT TTCACACATGTCGCAGGAGAAAAGGAAAATACCAATGTGGGACAGGACATGAGTTACCCGGGAGTTTCGG TCCTGGAAGTGGGTCTCGCAGACCTTGCAGTTGCCCGTCAGCAGGTCCACATGGTCCCGAGCATGCTGCC GGATCAGTTGAATGTTGGGCTCTAGAACTTTCTTGCACACCTGGCAGGGCATGGCCTTATTCTCCCGATT GTCATTCTCTCCAAAAGCCAACTCATCCTCACTGTCATCACTCAGCTCAATCACCTCCTCAGTTGTGCCT ATATCCTCAGCAGGGTCTTCAAACTGCAAGTCATCATCCCCCAGGTCCTCGAGCTGGAGCTCATCCATCT GCCCAAAGCCAGGATCTTTGGAGTGCTCACTATTGCTCAGACAGGAGTTGGTCCCAGTGGTAATGTCTAC TGCATTGTCAGGGGTGAGGAAGCTGTTTTTACTGAAGTCTCCATTGCTTTGAACTTTGTATGGCTGGCAG GAGCTCGTGCTGGTCTGGATGCCCGTGCTGACAGTGCCTAGGAGTGGCTGGGTCATCATGCTAGGAATGG ACGTGAAGGGAGCAGGGGTGTCATGGTCTTCTGTCTTTGGCGGTGGTGGGGGCGGTTGCGGCAGATGCAG GCTATGGTTAGCGTCACTGGCAACATTCTTCTCTTCCTCTTTATAGCTCCCTCCAGCATCACTGGGCAAC ACATAGTTTCTATCAGCACCAAGGTAGTCCCCACCACCTCGGAGTTCTCCAAGGGGATGGGCAGGTTCTG CCGAAGGACAACTCAGGGTACCAGAGTGGCTCTCACTGGTGCTGGTCAGGGGCTTGGCCCTGGCAGTGCT CTCCAGATCAGGAAAGGTAGAGTGACAGGCCTGGAGGAGGTCCTCCATACCCAGACGCTCAGCTACCTCG TAGATGACCCCAACATTGATCAGGTCTGTGAAGAGTTCTGAAGTGTAGACAAAGCTCAGAACCTTCTCAA AGTTGGCAGGGGTGATGAAGTCCACCACATAGGTCCTGGCAGCATCAAGCCCAGTATTCAGGAAGAGGTT CTGGAAGTAGCCAGCTGCACAGGCCAGCACAGCCTTGTGGGCCGGGAAGGAGCGGCTCCCCACCACAATG GTGACGTCGCACATGGTCTCTGAGAGCCGGCACTTGTTGAGTTCCTTCAGCAGGTTGTTGGGGTGGTTGG TGCTTTGCAGTTTGATCCTCATGCCCATCTTAGCAGCTCCTATGAAATTACCTCCTTATCAGCACAGTTA ATCTGTGGATAGCAAGAGAAAAAGATGGATGGCCAAACACATCCGTGCAAAAGAGGGGCTATGCAGGAGA AAATACCATCACTCTGAATGCTTCCAAGCCCCAGGTAGGGATCAGGAGTAAACTGGCATGGAGAGTTCCT CCATTATGACAAGGCAATGACATCTCCCCCTGTATATACTCAAAGAGTTATGACAAAAACCAAAACGACT GATGACAATCTGATAGCTTGGTTTTAAGCTGGTGCCTAGAGACGAACCAGAGGCATTCCCTTGGACACAA TCTTATACTTCATTACCAGAGCCACTGGATCAAATTTTTCTCCTATTAATTTCCTCCCAAATACAAGCTT TCCTAATTCATTGAGCATATTCTTTTCCCTCATGATGCCAAATACCATGCACATGCTTGAACACAACTAT CAAAGCTCCACTAGCTACTGAGGAACATATAAGGTGGTTCCGTTTTTTAGCTTTGTCATTGCTGTGTCTT CAACCTCTCAATAATCTCCAAATAAATCTCACGTTCTTGAATCCCCATCTCTCTCTGCCTATACTACATA CCCGCTCTCTGGCCTTTCGATAACCCAGAGCCCCTCCAGATATTGCTTTATCACAGTCCCTGTATTCTGT GAAGTTAAAACACTCAGAATGCACAGTAAGAAAAGTAGAACACAACTGTGGTAGTATTTAATATTACTTT AATATGTTGTATTCTTTTTCGCCACCCTTTTCGGGGTTTATATTTTGTTGTCCCTGCACTAACAGATTTA CTTGATGCCGCAAGAAAGACTTCTAATTATACAGGTAAGACTCCTAGAGGCTTGTTCACTACCACTTTCT GCCCTTTCTGACTCCTGCACGACTAAGGAAGAAGAGCTACTTGCATCCCTGTTCAGTCTCACAACTCTGC CCAACACAGCCCCTTTCTCCATCTCTCCAGATTCTAACAACTCCCAACTTTTTCTGGGGCCCAGGAAGCG TCTAAGTATTGTGGGGTACCGGCCAAGCATCTCCCGGGCCCTGGTGGCCACCCCTGAACGATGGGCGGGT CGGAGCACTGGGCAGCCAGAGGAGAGGGTGGGAGAGGGGGAAGCTGGGAACTTCCAAGGACACCCCAACA ATCCCCGGGCGCCGCGGTCTCCCCTCCGGAGTCGCATCTGGGAGGGAAGGGCGCTGCATCGGCTGGGGCG CTCCCTCTGGAGGCAACGAGCGCCTCAGTCCCTCCACAAACAGCGCGGGGCTCACCCGGGTGAGCCTGTT GGGGGCATGGGGCTGAATAAGGGGCCAAGCGAGGGGCGGCGGGGCCGGCCGGGGTCGGCGCGCGGGCGGG GAGGATGGGTCCGGCCGAAGGGAGCGGGCAAGAGGGACCCCAGCAAGAGCGAGATAAATGCCGGGCCGAA GGTCGGGCTTCGGCCAGGGGCAGCCCTCGGCCTGGCCGCGCTTACCCGGCTCTGCGGCGCCCCGAGCACC ATCGCCGCCGCCGCTTCACACAAAGGCCCCGGCCCGCCGTGCCCGGCCCGACGAGGAGGCGGCGCCGCCG GCCGCGGCCAGACTCTCCATCCGCCGCGCGGNNNNNNNNNNNNNNNNNNNNAAAACCCTATCCAAACTCT CTTCACCTCCCCTGTTCTACGCTCACAGTTCCAAAAGCTTCTCCTCAGAACATCTACTATGGGCATTTTA CAAAATGTTGTAAGATTGTTTGATTACTATATATCTCCTCCACTAGGCTGTGGGCTTCCTGAAGGCAGGG ATCTGTGGCTTTGCACACGGCCGCAGGTCCACACTGACCCTTACCACACTTCCTGGCACAATAAGTGCTT AATACATTAAGCCTGCTTGAATGAATGAAGGAGCACTTGAGGGCTAGACCGCTACTACTGGACCCTTCTG ATAATCCACTGCTGTCCCTCCTTCCATCTAGGTGGCCAAATGAAGAAAGTCACCGGATCTCACTGTACTT TGGCACCCCAGGCCTGCTTCCTGAAGCCTGCCCTCCCCAACTCTGTGTAGAACTCGATCACTCTTCCTCC CCACCCTTCTCCCAAATCATCTTTTCAAATGCTTCTGCCCAAGAATTGTCATCAAACGAAAACCACCTAC ACTGCCTGCCCCACCCACCACCACCAAAGACATTAGAAGTCTTCGTCATGTCGCCCTCCCTCCCTCAGTA CAGAGGAAGCTCTTAGGCTCCAAGACCCTTCAGTTTCATCCTCTCTCCCATCCCATCCCAGTCACTGCCT GAAAATAGCTTGCAGCCAAGGACAAACTCCCAGGACAGGCGCATGGTAGGATTCAGTAATTCCTTGATGG ATAAGTGGAAAGGAAGGGACACACTGAATGTTGCCAACAGATCTAAGCCAGTTTGGCCATTCAGCAATTG TCCATTCTCTTGATGAATTTGGGAGATTGTGTGTCCTCCTTTCCACAACCTCATTAACTGGTGGCAGACT ATACTGAAAGCAAGCACTATACCAAATGCTGCCAAACATGTAACATTTGTTAGCCAGGAGATACAAAAAC AGCCATAGAACTGTTCTTATAGTTTGGGGATGAGGCATCTCACTTTACCATCTAGTGAAAGGGTCACTTA GAGGTTGACACAATGTTTCAGTGGCAGGCCCAGCTCTCACAGTTGTGCAAGGTGACTGAGTCTCCCTAAA GTGGAATATCACACAGCCAGCAGGTCCAGACCTCCAGCATGACTCTGGAGACAGAGGGCTGCTGGGCTTG CAAGGGCCTGGGCCTATACAGTCTTTCTAACCAACTGCCCCAGCCATCTCTGGAGGGTTGACCCCAATAA ACTTCACATGAAAACAAATCATCCAAAAGACGCAGGTGAAAGTATATACCACTTATACTGAAGTCTTTTT AAAGTAAATCACCATATAGTCACAATAACAAACATATACAAAGTTAAATGGAGAAGAAAGGGCCAGGAAT TTAGTCAAGGTCTTGTGGACCAGATCTCTTTAGGCATCAACTGTTTTACAACAGGAAAGGGAGGGTCAGG ATCCCTTCACACATTCTTCAAGTACAAACACAGGAATCTAACTTAACATTTGTCTTCATTTTTTTCTGGA GTCCCCAGTACTGCAATTTTTGTCAGTCCCACTAGAGAGCAATCTTTCTGCACTTAATGTTCCTACTTGC AAAGCAATTGTCTCATCTCAGCCTATAAGCCTAACTCCCACCAAGTCTGAGCAGAACCAACTCTTGTAGC TTTCATGACCCAGAGAAAGCCCCTAGACTCTGCAAAAGTGTCCCCAGTTTTAAGCCCTATAGATAGATAA TTTTGAAAAGTCAAAGAGATGACTCACTGGAAAAAGGGTCTAGGCTCTGGGGTGGGGGCATTCTTTGACC TCTGGCTATAAAGCTGACATAAGTAATACTAGGGTGAGTATTCCCTCCTTTCCCCGCCCCCCACTATACA GCTGTCATTCCAAAGTGGCCCCAAGTCAGGCTGTAGGAAGATAGAATTTTCACTAGAATGAAAGGACTGC AGGAAGTGGCCTCTGGCTCTGTGGCCCTGTCCTTACACTGCCTTGGGAAATCTATGTGTTGATGTTTTAT CAGTGCAATTCAGGGGCATAACAAAGGCCCCTGTACCACGCTAAGATAAGGAGCCTGAGGATTTCACTGG CTGTTCCATTAGGGTACAGAACCCCCCTTAGGATGGCGGGTGATAGTTGACATCATAGATTAGTAAAAGA CATCCATACCCATGCATACCACATGTAAGTGCCTTACACATTCTGGGGACTGTTAAGTCAGAGCTGCAGG AGCTGGTAACAGGCACTTTAGAGAGGAGTGGGAGATAAATGTTTTCCCTTTATAATAACTAAGGCAGCCA GTTAACGGTGAAGATATCTGCTCCTCTCTTTCTTGGCCTCGGTAGTAACTTCCCTGTCCACATCCCATCC TAGCTGGATCAAGTTCTGTTTGCTTGTCCACAGCCCTGCTAAACTGTAAACTCTGTGTATGAGTCATCAT TTCATCCCCAGGATCTGTCACAGAACCCAGGCTCAGCATTTGGAGACAAAAAAATTGTATAGGGTCATAG ATTTGTCCTCCTCTGTGGCTGGGTCTCCGGACAGCCTTGATCCCCACCTCCTTCTCTAATTAAGTAGGGT CAGATTCAGGAATGAATATCTCCCAGACCCATCCTAGTAATACAAGTCATTCTGAATATTTTTTTTCTTT TTCTTTTTCTTTTTTTATTGTTTTTTTTGTTTGTTTGTTTTGTTTTGAGACGGAGTTTTTTGCTCTTGTT GCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAACCCCTGCCTCCTGGGTTCAAACGATTCT CCTGCCTCAGCCTCCCAAGTAGCTGGAATTACAGGCATGCGCCACCATGCCTGGCTAATTTTTGTATTTT TAGTAGAGAAGGGGTTTCACCATGTTGGTCAGGCTGGTCTCGACCTCCTGACCTCGTGATCCACCCGCCT CGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGGGCCCGGCCTGTTTTTTCTTTATCTAAAAC TGGTCTGTAAAAATCTTTGTGTGTTAGAAACCATCTTCCAAATTCGTGTATTTATTTTAATCACTAAATA AATCTGATACTCATCGTGCAAAGCACTGTGTAAGGCACTGTTCAGGAAATAGATATATACAAAAAGATAT GTAAAGTTATTTACATTTAAATATGAAGTGTGTGTATCATAAGTATGTGTGTATTTACCATGTAGCTGGA GGAGTCAAGGAACAGAAAAACCCACCAACCAAACAAGTAAACACACACACCACATCATTCATATGCAAAA ATCCTTTATTGTTGAGGAATAGAGAGAGACATTATATTTTTAATGTAGGACACTACAGGAACTCTAGGGT ATTCTACAGTCAATGCCCATTGGGGTTGGGGATATTCACTATGCAGTTTCCACACCAGGGTCAGGTAGAA AAGATGGAGAAGGAGTCAAAGCACAAGAATGTTACAAGAACAGGGAAGAGAAAGGGTAACAGGAGCGTGC GCACCTGGGGATTGGGACAGGGAAAGAGGTCTTCCTAATGCAGTCCAGGAGTCCGGAAGTGGAGTACTGA GGACAAAAAACAAAACAAAACAAAAAAAAACACTGAAATCACATCTTATATCCTGTAAAAAAGTCCCACT GAAAACCTGCTGTGGCAGCAGGAGAGAAGGGGAGAGGCGTCTGTGAGAGAGGAAAGAACAAGTCTTGACC CTCAGCCTGCCCTCCAGGCTTTGGGCCTGAGGATGGAACTCTCCTAGCCGCAGGTACTTGCACCTTCCCA GCTGCCATATTTACATTACTCTACACACCTGTGTGCATGGCCAGTGGGGCCCATGTGGACCAAAGATGTA GGAACCAAGTTCATGCCATCCTAGCACCACCATGAGCCAGACACACACACAGCCCTGCCCAAGGTGCCCT ACTATGAATGCTCCCCCTGCCTTATCCAGTTCCTCCTCTTCCCAGAAACCTCCCAGAGGAAGCACAGGCC CAGGGATTACTTACAGTTTCACATGAATGAAATCAGATGACACAATGATCTGTGTTGATCCATCTCATCT AATCTTTAAATGCCAGCTCCCAGAAACCTTATGTCCTATTCTGCCTGACACCTAACAGGTTGGGAATGCT CCCTCAATGGTAAGATTATGAAAGCTGCAATTATGGGTCCCTCTGAAAGAGCCTGGATGCACAAACAAGC ATTAAAAGTAGATTTCCACAATGCACTTGTTTTTGCCATTTGTATAAAATACTATAGCAAAATTGCACTG TAATTATCTTTATGGCCAGTGCATAACGCTGGAGCAGAGTATGTCATCCAGATATAGCTGCTCGTATAAT TATCTGGCCGTCGGCAGGACTGAAATAACCATCTAATGAGAGCAAAATGATACAGTAACTACCATGTAAT CAGCAAGTTACAGTTATTATAGCTCTTTGTGCCCTCTCACCCAACACTAAATGGGGGGCCAGACTCAGTC TCTGGTCCCAGGAATTCATGCACAGGCAGCATCATCAAGCACCAGCTCCTTGGGTCTTGGGCGTGCCACC TTGAGACCAGTGAGTATGGGTAACAGGGACACAGAGAGAAAAGCAGGAGGCTCTCCATGATTACGAGCCT TCCCAATCTCTCTCCCTCTCCTTTTCACTCCCTCCACCCCAATTCTTTGGAAGCTAGTGAAAAGAAAGCC ATTGGTGGAGCTGCTCTTGCTGTGTGTGGGAAGACAGTGTCAAGGCGGAAGGTTCGTGTTTTGACTAGAC ACTTCCCTGGGGGACTCTTACACTCGCATACCAAGTGAGGAATTTGTCAGCCCACTCAGGCCTGGGGTCC AGACAGAGCCAGTCAGGGGAAACATTAGGACATCCCCCATACCTCCCAAACTGGGACAATTATCATAAAA TTGGCACACAGCACTGTGCACCCGGCACTGTTCTGAACACTTTACCTATACTCAATCCTCAAAACCACAA CCCTGTGAGATACTATTATCACTTTCATTTTTCAGATGAGGAAACTGAAGCACAGGAGGATATGTACCTT GGCCAAAAAGATACAGATAGAGTGGCAGATCCAGGATTCACATTCAGGCCATTGTTCTTATTCATTATGC TAAAGAGAGATTCAGATATGGTTTCCTGCTAATCCCCATTAGATTTCAGAGTTCCTTGCATTTCTTCCTG AATCATCCTTGTGTATGACTACCTTCTGGGCCCCGTAACCCAGGATTCTGAGTGATTTGAAAGGCTCAGC CTGCCTGGGAGGCAAATGCTTGCCTGTGAATTGGTTGTGGTATTTGTAATTATCAGATAATAATTTCTCC ATCTTTTTGCATAGATTACAAAAGAATGAAGTCCAAACAACAGAAATTGCCCATCGGGGAAGCCCCTCTC TCCTCATCTCCCAACACTCACTCCTGCTCAATAGTCAGCTGCCGTCCATGATCCAGAGGAAAGAGATGAA AGACGGGGGATGTGTACAAACACACGTGCCTCTAAGTGTCTTTGTGTTTGGCCGACAGTGTGCTGGGCAT TGGCAGTGTATTTCAGGGCAAATCTATGAGCTTTAATACCTGTAGCATGGGAGGAAATGCACAGCTGGCA ACCCCCACAGCCCCCTCCCCAGTCCCCTCTCCCCTCTCTAATGAACAATCCTTACCCTATTCTGCTCTCG GGGGATACTTGAGGATGCCAAAGCTGGGGACGTTCTCTTGATTCACATCCGTAGGAGAGTCTAGGGTAAA GCGAACAGTGTAAGTAAGAGGGATCTTTCTGAATGTGAACTACACCCTGATCCAACAGCAAAGGGCTGGC TATGACGGGCAGTGTTCAAAGACAGAGTTGGGAGACTGGGGTGGAAGCAGA >gi|6005705|ref|NP_009195.1| G-protein coupled receptor 182 [Homo sapiens] MSVKPSWGPGPSEGVTAVPTSDLGEIHNWTELLDLFNHTLSECHVELSQSTKRVVLFALYLAMFVVGLVE NLLVICVNWRGSGRAGLMNLYILNMAIADLGIVLSLPVWMLEVTLDYTWLWGSFSCRFTHYFYFVNMYSS IFFLVCLSVDRYVTLTSASPSWQRYQHRVRRAMCAGIWVLSAIIPLPEVVHIQLVEGPEPMCLFMAPFET YSTWALAVALSTTILGFLLPFPLITVFNVLTACRLRQPGQPKSRRHCLLLCAYVAVFVMCWLPYHVTLLL LTLHGTHISLHCHLVHLLYFFYDVIDCFSMLHCVINPILYNFLSPHFRGRLLNAVVHYLPKDQTKAGTCA SSSSCSTQHSIIITKGDSQPAAAAPHPEPSLSFQAHHLLPNTSPISPTQPLTPS Vaximmutor GPX1 Homo sapiens 2876 41406084 AC121247 NP_000572 9606 3 49394608 49395790 - transcript variant 1 6.51 20647.83 203 Also known as GPXD; GSHPX1 >gi|224589815:49394608-49395790 Homo sapiens chromosome 3, GRCh37.p5 Primary Assembly GTTCTGCTGACACCCGGCACTTTATTAGTGGGGAAACTCGCCTTGGTCTGGCAGAGACTGGGATCAACAG GACCAGCACCCATCTCGAGGTGGTATTTTCTGTAAGATCAGGTGTTCCTCCCTCGTAGGTTTAGAGGAAA CACCCTCATAGATGAAAACCCCCCCGAGACAGCAGCACTGCAACTGCCAAGCAGCCGGGGTAGGAGGGGC GCCCTAGGCACAGCTGGGCCCTTGAGACAGCAGGGCTTCGATGTCAGGCTCGATGTCAATGGTCTGGAAG CGGCGGCTGTACCTGCGTAGGGGCACACCGTCAGGGCCCACCAGGAACTTCTCAAAGTTCCAGGCAACAT CGTTGCGACACACCGGAGACCAGGTGATGAGCTTGGGGTCGGTCATAAGCGCGGTGGCGTCGTCGCTGGG AGCTGGCAGGGCCTCCCGCAGGAAGGCGAAGAGAGGGTGCGCCCCCGCACCGTTCACCTCGCACTTCTCG AAGAGCATGAAGTTGGGCTCGAACCCACCACCAGGCCGGACGTACTTGAGGGAATTCAGAATCTCTTCGT TCTTGGCGTTCTCCTACAGGAGAGAAGGGCAGCTAGAACCCGGGGTCAAGAGGAGGAGAGAAACGTTCTA ACCACAAACAAGGGAGATTTTCTATGAGTCACCGGGATTTTGCCCTCCATGCGCAATCCCAAGGGCGGAG AGGAATTTCAGCAGCTACGAGCAACAGAAAGGAAACGAGAGAGTAGCCAGACTCTCCGCGCATGGAGCCG ACGGCACCCACCAGCACACCGCCGGCGCCCCCAGCCACTACTGCACGTCCGCCCCCGCCCCGCCCCGCTC CGCCCGGCGCACCTGATGCCCAAACTGGTTGCACGGGAAGCCGAGCACCACCAGGCCCCGGGGTCCGAGG CGCCGCTGCAGCTCGTTCATCTGGGTGTAGTCCCGGACCGTGGTGCCTCAGAGGGACGCCACATTCTCGA TAAGTAGTACCTTGCCCCGCAGGGAGCCCAGGCTCACAGGCTCCCCGCCGGCCAGCGGGCGCGCCGAGAA GGCATACACCGACTGGGCCGCCGCCGCCGCCGCCGCTAGCCGAGCAGCACACATGGCGCAATTGTCCAAG AAGCCAGCGGAGCGCCCCGAACAAGCACTGTAAGGGGAGGCCAGCAGGCGCCTCCTTTTAACT >gi|41406084|ref|NP_000572.2| glutathione peroxidase 1 isoform 1 [Homo sapiens] MCAARLAAAAAAAQSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLUGTTVRDYTQMNELQRRLGPRG LVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDA TALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLSQGPSCA Vaximmutor grpE Mycobacterium tuberculosis H37Rv 886497 15607492 Rv0351 AL123456 NP_214865 83332 421708 422415 + stress response protein GrpE 4.17 22152.06 235 HSP-70 cofactor >NC_000962.3:421708-422415 Mycobacterium tuberculosis H37Rv, complete genome AGTGACGGACGGAAATCAAAAGCCGGATGGCAATTCGGGCGAACAGGTAACCGTCACTGACAAGCGGCGG ATCGATCCCGAGACGGGTGAAGTGCGGCACGTCCCTCCCGGCGACATGCCGGGAGGGACGGCTGCGGCCG ATGCGGCGCACACCGAAGACAAGGTCGCCGAGCTGACCGCCGATCTGCAACGCGTGCAGGCCGACTTCGC CAACTACCGTAAGCGGGCGTTGCGCGATCAGCAGGCGGCCGCTGACCGAGCCAAGGCCAGCGTTGTCAGC CAATTGCTGGGTGTACTGGACGATCTCGAGCGGGCGCGCAAGCACGGCGATTTGGAGTCGGGTCCACTGA AGTCGGTCGCCGACAAGCTAGACAGCGCGTTGACCGGGCTGGGTCTGGTGGCGTTCGGTGCCGAGGGCGA GGATTTCGACCCCGTGCTGCACGAAGCGGTGCAACACGAGGGCGACGGCGGGCAGGGGTCCAAGCCGGTA ATCGGCACCGTCATGCGGCAGGGCTACCAACTGGGTGAGCAGGTGCTGCGGCACGCCTTGGTCGGCGTCG TCGACACGGTGGTCGTCGACGCGGCCGAACTGGAGTCAGTCGACGACGGCACTGCGGTCGCAGATACCGC CGAAAACGATCAAGCTGACCAGGGCAATAGCGCCGACACCTCGGGCGAACAGGCAGAATCAGAACCGTCG GGCAGTTA >NP_214865.1 stress response protein GrpE [Mycobacterium tuberculosis H37Rv] MTDGNQKPDGNSGEQVTVTDKRRIDPETGEVRHVPPGDMPGGTAAADAAHTEDKVAELTADLQRVQADFA NYRKRALRDQQAAADRAKASVVSQLLGVLDDLERARKHGDLESGPLKSVADKLDSALTGLGLVAFGAEGE DFDPVLHEAVQHEGDGGQGSKPVIGTVMRQGYQLGEQVLRHALVGVVDTVVVDAAELESVDDGTAVADTA ENDQADQGNSADTSGEQAESEPSGS Protective antigen HBHA Mycobacterium tuberculosis H37Ra VO_0012373 5211814 148660242 MRA_0482 CP000611 YP_001281765 25177 567106 567705 + iron-regulated heparin binding hemagglutinin Hbha 9.84 20541.79 199 >NC_009525.1:567106-567705 Mycobacterium tuberculosis H37Ra, complete genome CATGGCTGAAAACTCGAACATTGATGACATCAAGGCTCCGTTGCTTGCCGCGCTTGGAGCGGCCGACCTG GCCTTGGCCACTGTCAACGAGTTGATCACGAACCTGCGTGAGCGTGCGGAGGAGACTCGTACGGACACCC GCAGCCGGGTCGAGGAGAGCCGTGCTCGCCTGACCAAGCTGCAGGAAGATCTGCCCGAGCAGCTCACCGA GCTGCGTGAGAAGTTCACCGCCGAGGAGCTGCGTAAGGCCGCCGAGGGCTACCTCGAGGCCGCGACTAGC CGGTACAACGAGCTGGTCGAGCGCGGTGAGGCCGCTCTAGAGCGGCTGCGCAGCCAGCAGAGCTTCGAGG AAGTGTCGGCGCGCGCCGAAGGCTACGTGGACCAGGCGGTGGAGTTGACCCAGGAGGCGTTGGGTACGGT CGCATCGCAGACCCGCGCGGTCGGTGAGCGTGCCGCCAAGCTGGTCGGCATCGAGCTGCCTAAGAAGGCT GCTCCGGCCAAGAAGGCCGCTCCGGCCAAGAAGGCCGCTCCGGCCAAGAAGGCGGCGGCCAAGAAGGCGC CCGCGAAGAAGGCGGCGGCCAAGAAGGTCACCCAGAAGTA >YP_001281765.1 iron-regulated heparin binding hemagglutinin Hbha [Mycobacterium tuberculosis H37Ra] MAENSNIDDIKAPLLAALGAADLALATVNELITNLRERAEETRTDTRSRVEESRARLTKLQEDLPEQLTE LREKFTAEELRKAAEGYLEAATSRYNELVERGEAALERLRSQQSFEEVSARAEGYVDQAVELTQEALGTV ASQTRAVGERAAKLVGIELPKKAAPAKKAAPAKKAAPAKKAAAKKAPAKKAAAKKVTQK Protective antigen Purified preparations of HBHA were emulsified in a dimethyldioctadecylammonium bromide-monophosphoryl lipid A adjuvant and tested for the ability to reduce M. tuberculosis infection in the mouse aerosol challenge model for tuberculosis. The HBHA-containing vaccine gave a approximately 0.7-log reduction in CFU in both mouse lungs and spleens compared to adjuvant controls 28 days following challenge [Ref1482:Parra et al., 2004]. HIST1H2BE Homo sapiens 8344 21396484 AF531288 NP_003514 9606 6 26184023 26184457 + histone cluster 1, H2be 11 13013.18 126 Also known as H2B.h; H2B/a; H2B/g; H2B/h; H2B/k; H2B/l; H2BFH; H2BFN; dJ221C16.8 >gi|224589818:26184023-26184457 Homo sapiens chromosome 6, GRCh37.p5 Primary Assembly CATGCCTGAGCCAGCGAAATCCGCTCCCGCCCCGAAGAAGGGCTCCAAGAAGGCCGTGACCAAGGCGCAG AAGAAGGACGGCAAGAAGCGCAAGCGCAGCCGCAAGGAGAGCTACTCCGTATACGTGTACAAGGTGCTGA AACAGGTCCACCCCGACACCGGCATCTCCTCTAAAGCCATGGGGATCATGAATTCCTTTGTCAACGACAT CTTCGAGCGCATCGCCGGCGAGGCTTCCCGCCTGGCGCATTACAACAAGCGCTCGACCATCACCTCCAGG GAGATCCAGACGGCCGTGCGCCTGCTGCTTCCCGGGGAGCTGGCCAAGCACGCTGTGTCAGAGGGCACCA AGGCCGTTACCAAGTACACCAGCTCCAAGTAAACTTGTCCCTGCAACTGCCTTAGTAAACCCAAAGGCTC TTTTCAGAGCCACTC >gi|21396484|ref|NP_003514.2| histone H2B type 1-C/E/F/G/I [Homo sapiens] MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDI FERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK Vaximmutor HIST2H4A Homo sapiens 8370 4504323 AF525682 NP_003539 9606 1 121215050 121215445 - histone cluster 2, H4a 11.91 10474.58 103 Also known as H4; H4/n; H4F2; H4FN; FO108; HIST2H4; HIST4H4; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4B >gi|157704448:121215050-121215445 Homo sapiens chromosome 1, alternate assembly HuRef, whole genome shotgun sequence GTGGTCGGCCCTAAAAAGGGCCTTTGGGATCGAAACGTGCAAAGCTGGAGCGGCGGCCTAGCCTCCGAAG CCGTACAGGGTGCGCCCCTGGCGCTTGAGCGCGTACACCACATCCATGGCTGTGACGGTCTTGCGCTTGG CGTGCTCGGTGTAGGTGACTGCGTCCCGAATCACATTCTCCAGGAACACCTTCAGCACACCGCGGGTCTC CTCGTAAATGAGGCCAGAGATCCGCTTAACGCCGCCACGCCGAGCTAGACGCCGAATGGCAGGCTTGGTG ATGCCCTGAATGTTGTCTCTCAAGACCTTGCGGTGGCGCTTAGCGCCCCCTTTGCCTAAGCCTTTTCCGC CCTTTCCTCTGCCGGACATGACCGCTGGAGCCCGATAGACAGCTTC >gi|4504323|ref|NP_003539.1| histone H4 [Homo sapiens] MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDA VTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG Vaximmutor hspX Mycobacterium tuberculosis H37Rv 887579 15609168 Rv2031c AL123456 NP_216547 83332 2278497 2278931 - alpha-crystallin 4.75 15135.96 144 Also known as acrheat shock protein HspX; alpha-crystallin homolog (14 kDa antigen) (HSP16.3) >NC_000962.3:2278497-2278931 Mycobacterium tuberculosis H37Rv, complete genome GTCAGTTGGTGGACCGGATCTGAATGTGCTTTTCGGTTGGCTTCCCTTCCGAAACCGCCACCGACACAGT AAGAATGCCCTTGTCGTAGGTGGCCTTAATGTCGTCCTCGTCAGCACCTACCGGCAGCGACACCGTGCGA ACGAAGGAACCGTACGCGAATTCCGAGCGACCGTCGAAGTCCTTCTGCTCGGTGCGCTCGGCCTTGATGG TCAGCTGACCATCGCGGACCATAATGTCGACGTCCTTGTCGGGGTCGACCCCGGGAAGCTCCGCGCGTAC CTCGTAGCGCCCCTCTTTCATCTCGTCTTCCAGCCGCATCAACCGGGTGTCGAAGGTGGGCCGGAGTCCG GCGAATGACGGGAAGGCCGCGAACAGCTCAGAAAACTCGGGGAAGAGGGACCGCGGGTGGCGCTGAACGG GAAGGGTGGTGGCCA >NP_216547.1 alpha-crystallin [Mycobacterium tuberculosis H37Rv] MATTLPVQRHPRSLFPEFSELFAAFPSFAGLRPTFDTRLMRLEDEMKEGRYEVRAELPGVDPDKDVDIMV RDGQLTIKAERTEQKDFDGRSEFAYGSFVRTVSLPVGADEDDIKATYDKGILTVSVAVSEGKPTEKHIQI RSTN Protective antigen htpG Mycobacterium tuberculosis H37Rv 887501 15609436 Rv2299c AL123456 NP_216815 83332 2570058 2572001 - chaperone protein HtpG 4.51 68991.53 647 heat shock protein (HSP90 family protein) (high temperature protein G) >NC_000962.3:2570058-2572001 Mycobacterium tuberculosis H37Rv, complete genome GCTACAAGGTACGCGCGAGACGTTCGGCCAGCAGCTCGGCGAACCTCGCCGGATCCTCGAGTGCGCCGCC TTCGGCGAGAAGCGCTGTGCCGTAAAGTAATTCCGCGGTTTCGGCCAATGATTTCTCGGCATCGTCTGCG CGGTCCTGGTGGGCTTGGCGCAGGCCGGTCACCAACGGATGGCTCGGGTTGAGCTCAAGTATCCGCTTGC CGACCGGAACCTCCTGGCCGGAAGCCCGGTAGATGCGCGCGAGCGCGGGTGTCATCCCGAAGGCATCGGT GATCAGACAGGCCGGTGACTCGGTCAGGCGGGTGGACAGCCGCACCTCCTTGACGTGATCGCTCAACGTC TCCTGCAACCAGGTCAGCAGGTCGGCAAATTCCTTCTGCCGCTCCTCGCGCTCGGCCTCGCTGGTGTCCT CTTCGGAACTCAAGTCCACCTCGCCCTTGGCAACCGACTGCAGCGGTTTGCCGTCGAACTCCGGCACCAT TCCCACCCAGACCTCGTCGACCGGGTCGGTGAGCAGCAGCACTTCGTACCCCTTGGCCTTAAACGCCTCC AGGTGCGGTGACTTCAGCAGTTGTTGGCGCGTCTCGCCGGTGGCGTAGAAGATCTGTTGCTGACCGTCCT TCATGCGCTCGACGTATTCGGCCAGCGTGGTGGGTTCCTCCTCGCTGTACGTGGAGACAAACGAAGAAAT ACCGAGCAGGGTCTCCCGGTTATCGATGTCTGACAGCAGTCCCTCTTTGAGGACCCTGCCGAACTGTGTC CAGAACGTGCGGTAGTCCTCCGGCCGGCTGGACTGCACGTCCTTGATCGTGGACAGCACCTTCTTGGTCA GCCGCCGGCGGATGGCCTTGATCTGCCGGTCCTGCTGCAGGATTTCGCGAGAAACGTTGAGCGACATGTC CTGCGCGTCGACCACACCCTTGACAAAACGCAAGTACTCGGGCATGAGCTGGTCGCAGTCGCCCATGATG AACACCCGCTTGACGTAGAGCTGGATACCGACGTGGGCGTCCCGGTCGAACAGATCGAACGGGGCATGAG ACGGGATGAACAGCAGGGCCTGGTACTCGAAGGTGCCCTCGGCCTTCATCGCGATGATCTCGAGCGGGTC GTCCCAGGCGTGCGCGACGTGTTTGTAGAACTCCTTGTACTCCTGCTCAGACACCTCTTCTTTGGGCCTC GCCCACAGCGCCTTCATCGAGTTGAGGGTTTCGGTTTCGATGGTGACGGTCTCCTCGCCGCCTTCCCCCC CTTCTTCCTGGGAGGCTGGGGTGCGGCGCTCGACGTCCATCCGGATGGGCCAGGCGATGAAGTCGGAGTA TTTCTTGACCAGGTTACGGATCTTCCATTCCGAGGTGTAGTCGTGCAGGTCGTCCTCGGCGTCTTCCGGC TTGAGGTGCAGGGTGACCGACGTGCCCTGGGGGGCATCCTCGACGGACTCGATGGTGTAGGTGCCCTCAC CGCTGGACTCCCATCTGGTGGCCGCGCTCTCGCCAGCCTTGCGGGTAAGCAGTTGGACCTTGTCGGCCAC CATGAACGACGAGTAGAAGCCGATGCCGAACTGACCGATCAGTTCCTCGGAGGCGGCCGCGTTCTTGGCC TCACGCAGCTGTGCGCGCAGCTCGGCGGTGCCCGACTTGGCCAGCGTGCCAATCAGATCCACCACCTCCT CGCGCGCCATCCCGATGCCGTTGTCACGAACGGTAAGAGTCCTTGCAGCTTTGTCTGCGTCGATCTCGAT GTGCAGATCGGAGGTGTCGACCTCCAGGTCCTTGTTCCGCAGCGCCTCAATCCGCAGCTTGTCTAGCGCA TCGGAGGCATTCGAGATCAACTCCCGCAGAAACGCGTCCTTATTGGAGTAGACCGAGTGGACCATCAAAT CCAGCAGTTGCCGGGCCTCCGCCTGAAACTCCAACTGCTCGACATGGGCGTTCA >NP_216815.1 chaperone protein HtpG [Mycobacterium tuberculosis H37Rv] MNAHVEQLEFQAEARQLLDLMVHSVYSNKDAFLRELISNASDALDKLRIEALRNKDLEVDTSDLHIEIDA DKAARTLTVRDNGIGMAREEVVDLIGTLAKSGTAELRAQLREAKNAAASEELIGQFGIGFYSSFMVADKV QLLTRKAGESAATRWESSGEGTYTIESVEDAPQGTSVTLHLKPEDAEDDLHDYTSEWKIRNLVKKYSDFI AWPIRMDVERRTPASQEEGGEGGEETVTIETETLNSMKALWARPKEEVSEQEYKEFYKHVAHAWDDPLEI IAMKAEGTFEYQALLFIPSHAPFDLFDRDAHVGIQLYVKRVFIMGDCDQLMPEYLRFVKGVVDAQDMSLN VSREILQQDRQIKAIRRRLTKKVLSTIKDVQSSRPEDYRTFWTQFGRVLKEGLLSDIDNRETLLGISSFV STYSEEEPTTLAEYVERMKDGQQQIFYATGETRQQLLKSPHLEAFKAKGYEVLLLTDPVDEVWVGMVPEF DGKPLQSVAKGEVDLSSEEDTSEAEREERQKEFADLLTWLQETLSDHVKEVRLSTRLTESPACLITDAFG MTPALARIYRASGQEVPVGKRILELNPSHPLVTGLRQAHQDRADDAEKSLAETAELLYGTALLAEGGALE DPARFAELLAERLARTL Protective antigen human IFNG Homo sapiens 3458 56786138 AC007458 NP_000610 9606 12 68548549 68553520 - interferon, gamma 10.01 18454.87 166 Also known as IFG; IFI >gi|224589803:68548549-68553520 Homo sapiens chromosome 12, GRCh37.p5 Primary Assembly AGTTGTGAACTTACACTTTATTCATATATATTAGATATTGATAATTTTAACAAATGAGTTACTTTCCATT TGGGTACAGTCACAGTTGTCAACAATATTTGGAAGCACCAGGCATGAAATCTCCTGAGATGCTATGTTTT CATCAGGGTCACCTGACACATTCAAGTTCTGTCTGACATGCCATTAAAGCACTGGCTCAGATTGCAGGCA TATTTTCAAACCGGCAGTAACTGGATAGTATCACTTCACTTATAAGTGTTCATTGTATCATCAAGTGAAA TAAACACACAACCCATGGGATCTTGCTTAGGTTGGCTGCCTAGTTGGCCCCTGAGATAAAGCCTTGTAAT CACATAGCCTTGCCTAATTAGTCAGAAAACAAAGGATTAAGTGAGACAGTCACAGGATATAGGAATTATA AATAATACATATATTAATAGATATTCATTTTCATTACACAAAAGTTGCTATTATAAATACTTATTTGATT GATGAGTCTAAAAATATATTCCCCATATAAATAATGTTAAATATTAATAAATAGATTTAGATTTAAAATT CAAATATTGCAGGCAGGACAACCATTACTGGGATGCTCTTCGACCTCGAAACAGCATCTGACTCCTTTTT CGCTTCCCTGTTTTAGCTGCTGGCGACAGTTCAGCCATCACTTGGATGAGTTCATGTATTGCTTTGCGTT GGACATTCAAGTCAGTTACCTATCGGGAAAGAAAAGAGCAAAATTAATTTCAGGCATATAAGCCATCAGG ATATTCTGCTAATGTCATGATGGTCAGTGAAAATAAAAGTATTCCTTTAAAAAATGGACCGTATTACCTT AATATACAATATTTACTTCTTCAGCAGTTGAAGCTAAAAATTACAATTACATTTCTAGTTGTGCTATAAT GTGGTGGAGTCCATCACATATCTTTAAATCTACTTCTATAGAATTCTTGAAATTATATAGTGAAAAGACT CCTGGTTGTGGAGTCAGCATAAAAAGGTGGGTTTGAATCTCATGCATGCAATAATTCTGACCTTGAGCCA GCTACTTAACCTCTCTGAACTTCTATTTTCTCATCTAAAGGAGGATGAGACTGTTTCTATGACCTCTCTA TCATCAATCTACCAATTATATCACTTTATACTTCAGTCTTTCCTGTATGCATCAAATGAGTAACTATAGG TCAATGATTCTCACTGTTGTGCATAGCAGATTAATCCGGAAACCTGTCAAAGACCTCAGAGATCCAGGTG CATGAAGTGGAAGGACTGTGCCCTGTGCATTCACTAAGCTCCCCAGGTGATTCTGACATCCACCTAAGTT TGAGAACCACTGATCTAGGTTAGGTACCCTAGAAAACACCAAATCCAAAACGAGTGAAAACTGTAATTTT TAATTTCTCCCAGATTAGGTCAGTCTCTCAATTTCCATAATAGATTTTAAAATAAACACCCCCAATCATA TTTATATATGGCTTACTGATATATAAAGTTTAAAATTAAGAATTGCAACAACTTTTCCAGTACCCTGCCT TCTAGGCAAGCAAATTGAACTACTTGCATCTCCTCACTCTAACCAATAGGGCCATTTAGATGATGCTTCA TAAAATGTGGACCATTTACCTTCCTCTTGGCTCTGCCATTACATTTTTTCTAATCTTGGGAACACTATGG CTACTCACCACATTTCTGAGGATGGCTGGGGGATTACAGCTAAAGACTAATTGTCAAAGGGCATAACATG CATAAAATAGCTTAGCAACTGAGCCAAAGAGAATAATAATTAATAGAGCTCACTCAAGATTGCCCATCAA GAAACAGGAGGTTTGTAGCTTCCAGAAGGAAGAAACCACAGATTTTTTTTCATTTTTTTAAGGCCCAGTT CCTGCAGAGTAGAAAAAATTTTCGACAAGCTGTGTGTTTGCAAGTGACAGATTTTACTTTCCAATGTTCA AATCATCACCAAGAAACTAAGAGACTTATCCAGGACTGAACAGTAAGCCAGGGGCACCACTGGATTAGAA ACAAGTGCTGAGTGTCTAGTTTATAACCAAAGGAGACCTGTTGCCATGTCTCTCCTTTCTGTTTCTGGGG GCTTACATGAGAGGAGTGAGAGGCAGGCCCAGCAATAGTGAAAGCTGAGCACATATCCTAAGAACACAAA TGCTAGCTTGAAGACCCTGGTCCCTCTCATTTGAGCCCATTTATATCAAAGTCAACCCCAAAGGAATCTA GAGGCCTTTGTCTTCTAGAAAACATTTTGAAAAACATGATTTGTCTTTATGCCTCAACGTTATCTTTCTG TTTATAGAAGCCATAACTTAAAGATACAAGTTCATGAGGTAAGACTTAGGAGTCTAAGGAGTTCTCCAGC TGAGATATTCTGAAAGTTGATAGAGAGTTGATAGAACAATCTTTTCATAAGGTATAAATTCTATCTATTT TTCCCTAAAAACAAACAAACAGCAACCATTCTTGCTTCTAATTGGGCAGTACAATCTGATAGGTTGGCTA GAGACTTGCAGTGGGGTGTCCCTGGTACCTATTCAAAGACTGTAGCTTTCTTCTATCTCATTCTCATTTT CTATTCTTTGCATTGTAGAGTTTTGGAGCAAAGAAGGTCATCAAACTTATACAGTGAGCCTAACAGTTTC CTTTTAAGATGAGGAAACTGAGCCCCAGCCAGCCATGTGATTCATCACAGTTCCTTGGTGGCTGAGTTGG GAGGAGAACACACATCTTCTCAGCTCCTCCCACTGCTCTTTCCATTAAGACAGACAGCCTCTCATTCAAA GTAAGAGAATTTCCATCATATGAGCAAGGGACAATGAGAGAACTGCTTCTCAGTACTCCCCGCTTCTTCC TCACCTACTTCCTCTTCACTGGATTTGTCAACTCACCTGTCTTTACGCAATAGTTACAATGCCAGCATTT CTCTACATTACATACTTCAGCGATTCTCTTACTGGCTTTGCAAAGTCACCCAAACACGAATGGAAATAGT AAGGTAGAGTTTCAGCCATGAGTTGAGTTCATAGCTTTAGCAACTGTTAAATAGCTAATGTCTACTTTCT GGAGAATAAATGCTTTGCAAGACCCTCGGCAATGAAACCAAAGAAAGAATTTAAATAGCCTCACCGAATA ATTAGTCAGCTTTTCGAAGTCATCTCGTTTCTTTTTGTTGCTATTGAAAAACTTGACATTCATGTCTTCC TTGATGGTCTCCACACTCTTTTGGATGCTCTGGTCATCTTTAAAGTTTTTAAAAAGTTTGAAGTAAAAGG AGACAATTTGGCTCTGCATTATTTTTCTGTCACTCTCCTTGGAAGGAAAGAGCACAAACAGAGGATGATG TGAATTTATCCATCAGAAAGCAAGCAACAGGAAAATTAGCCAAATGGGAATATTCAGCTTACCTCTTTCC AATTCTTCAAAATGCCTAAGAAAAGAGTTCCATTATCCGCTACATCTGAATGACCTGCATTCTAAAAAAA AAAAAAGAAAAAATTGGTTTACAATTAGCCCATAAATTGCCTTAAAAATATATTTCAAGTTTCATTGAAC TCAGATGTGACAATATTCACTGATTTCCTTTTCAACTCTTCTGCTTAGTTCTAACAATAAGTATTCCCAA AAGGCTTATGTGAGATATAGACAAAGACTATTATGTTCTTTTAGCTTTTTTATTTCCCAATATAACCATT AAATTGCAATGTCACAAATGAGCTCAACAAAGCTGATACTCCAAAGGTCCCAAAAACTATAGTAAGCTAA AGAAAGTATTTTCAAGCTAGGCTAAAAAATACAAAAACAAAAAACAGCAAAGCCACCCCACTATAAAATA CTGCCCCCCAATGGTACAGGTTTCTATTACATCTACTGTGCCTTCCTGTAGGGTATTATTATACGAGCTT TAAAAGATAGTTCCAAACATGTGCGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGATTTGATTTTGTGTT GTAAGAATAAAATCAATTGTGAATGTCTGAATATTATTATGACAGCTATAATTATAACAGCTAAGTTTTA AACAAATAATGAAATATTTAAAATGTAAAAACTTTTCATTAACCATCAATAAAATTTTAAAAGAAACTCA AAAAATTCACTTAAAATTTTTAAACATTCCTTAGCCAGAATCATAATTCTTCCAAAATCCCAGTGAGCAA CGAAGTTCCGGAATACTTCACAAGTAATATAGATAGAGAAGCAGAATGTTGAAATCACTTTTTGGAGAAA ATCACCTATCAGTTATTTTTTTAAGCCAAAGCTTTAAGGACCTTTTTGACTTTAATTTGTAAACATTTCC TATTACCTTTTTAATGCTAATCTTGACCTTAGAGTCTGAGAAGTCAAAAACTAATAACCAATCATTTTTT TCAATCTGCATACTCCTTGACTATCACAACTGAATGAGTTCCCACCACAAAATTATTAACATCATTATGC TTCATACCCATATTATGCCCATCTTTTGGAAAATTCATAAATCCCTTTTAAGTCTCTATACAAACTGAGC AGAAGGTTAAAACAATATCTAGATTATTTAGTCAAGGAACTCATCTAGTCAATAATTAACAAAATAACAC CAAATCTCAAAATGACTGCCTACAAGAGATGACAGCCTATCAGAGATGCTACAGCAAGTCGATATTCAGT CATTTTCAACCACAAACAAGTACTATTAAAAAGTCATACTTACAAAATATTTCTTAAGGTTTTCTGCTTC TTTTACATATGGGTCCTGGCAGTAACAGCCAAGAGAACCCAAAACGATGCAGAGCTGAAAAGCCAAGATA TAACTTGTATATTTCATCGTTTCCGAGAGAATTAAGCCAAAGAAGTTGAAATCAGTAGTTCTTGTATCAA GCTGATCAGGTCCAAAGGACTTAACTGATCTTTCTCTTCTAATAGCTGATCTTCAGATGATCAGAACAAT GT >gi|56786138|ref|NP_000610.2| interferon gamma precursor [Homo sapiens] MKYTSYILAFQLCIVLGSLGCYCQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQS QIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVM AELSPAAKTGKRKRSQMLFRGRRASQ Vaximmutor IFNG Bos taurus 281237 31982949 BOS_5228 AF140653 NP_776511 9913 5 45830157 45834980 + interferon, gamma 10.24 18866.5 166 >gi|258513362:45830157-45834980 Bos taurus breed Hereford chromosome 5, Bos_taurus_UMD_3.1, whole genome shotgun sequence AATTAGAAAAGAAAGATCAGCTACCTCCTTGGGACCTGATCATAACACAGGAGCTACCGATTTCAACTAC TCCGGCCTAACTCTCTCCTAAACAATGAAATATACAAGCTATTTCTTAGCTTTACTGCTCTGTGGGCTTT TGGGTTTTTCTGGTTCTTATGGCCAGGGCCAATTTTTTAGAGAAATAGAAAACTTAAAGGAGTATTTTGT AAGTATGATCTTTTAATGAGACTTTCTTGTGATTGAAATGACTTGATGTTTGATGTTGACTTGGAATTCT ATCTATGATGGGCTCTCATCTCTAGTCTTCAGTCATTTTGAGAAGACTTGCTGTTATTGTGACTGTTAGC TAGATGTGTTTGTTTGTTTGGTTTTTTTTTTTTTTCTGACTAAACAATCTCTGCTCAGTTTGCTATAGAG ATTTAGAAGGGATTCATGAATCTTTCAAAAGATGGGCATAATATGTGTATACATCATAGTGTTGATATTT TGTGATAAGAATCCATTCAACTATGATGTTAAGTAGCAAGCATACTGAAAAGACAGCTGAGTCTTAATTT TTGATTTCTCATGTTACTATAATGATGACAGTCAGAAGTCAAGATCAGCATTAAAAATGGTCATTTGACA TAATTGATCAATTAAAGAAGATGAGGTCCTTAAATATTTGGCTTAAAAACAAAAACACTTATAGGTGTTT TTCTCTAAAAAGTCATTTTAAAATTTTACTATCCCATCTAGATTGTCTGTGAATTACTCTTGGACTCAGT CACTTGCTGAGATTTTTTTTGTAGGAGTTATAATCTAGTTTATATTTTAAAAAATTTTTTGTCTTGTTTT AGGAATTTCTTAAAAATTTACTTATGGTTAATTAAAATAGCTTGGGCATTTTAAATATTTTATCATATAT CCAAATTTAGCTGTTATAATTATAGCTGGAGCTATCTTTTAAAGGCGATATAATACCTTATAGGAGGACA CAGGTAGAGGTTATGAACCGTATACCATAGGGGGGCAGTATTTTATAGTGAGATGATGTTGTTGATTTTT TTTTTTTTTTTAACTATACCTTTTTCTTACCATAGTAATTTGGGACAGTTGAAGTATCATCAGTCTTGAA TGAGTTCATTGTGATGTTATATTAGTAAATAAGAAAGCAAAAATCACATGATAGTCTTTGGCTGTTATCC AACCAGGTAGCTTTTGTGAATATTTTTTGTTAAAACTAAACAGTGTTGAAAAGAAAATCAGTAAATAGTG CCAGCATCCAAGTTCAATAAAACTTGAGGTACATTTTTACAGCAATTTATGGACTAATTTTAAGCCAATT TTTTATTGTGTTGTTTTATTTTCCAGAATGCAAGTAGCCCAGATGTAGCTAAGGGTGGGCCTCTCTTCTC AGAAATTTTGAAGAATTGGAAAGATGTAAGCTGAACATTTCCATTTGGCTGATTTTCCTGTTGCTTTTTT TCTGATGGATAAATTCACATCAACCTCTCTTTGTGCTCTTTTCTCCCAAGGAAAGTGACAAAAAAATTAT TCAGAGCCAAATTGTCTCCTTCTACTTCAAACTCTTTGAAAACCTCAAAGATAACCAGGTCATTCAAAGG AGCATGGATATCATCAAGCAAGACATGTTTCAGAAGTTCTTGAATGGCAGCTCTGAGAAACTGGAGGACT TCAAAAAGCTGATTCAAATTCCGGTGAGATGATCTTAATGCTTTCTTTGGTATCATTGCAGAGGCTCTTA CAAAGCACTTCATTCCCAGAAAATAGAAATTAGATACCAGGTGTAAAGCTCTGAAGTGAACTTGTGGTCA AAATGCCTCTTTGCTAGTTCTCTTTTGACTTGGGTGACTTTGCCGAGTCAGTTATTGGAGGCACTTAAGT CTGTGATTTTGGGAAAATACTGGCATTATGCCTATCGCACAAAGATGGGTGAATGGACAAATCAGTGAGG AGGAAGCAGAGAAGGAGTTGGGGTGATGGGGACAGAGTACTGAAAACCAGTTCTCCCATTGCCCCTTGTC TGTGTGTTGGAAATTCTCTTGCTTTGAATAGGTTGCATGTCTTAATGGAAAGAGCAGTGGGAGGGAAAGG GAAGAAGATGTGTGCTCCGCCCAGCTTAGCCACCAAAGAAATGTGTGACTTCAGATGAATCACAGGCCTG GCTGGGGCTGTTTCCTCATCTTAAAAGGAGCCTGTTGAGTTCACTGTAATTTCTACGGTCTCCTTTGCTC TAAAATTCTACAATGCCATGGAAAGAAAATGAGAATGAGATAGGAGAAAGGAATAGCCTTTGAGGAGACA AAGGCCCTCCCACTGTCGAACTCTGGTCAATCACCCAGATTGTATAGCCCAAGGAGAGGTGACTGCGTTT GTTTTTAGAAAGAAAATAGGTAGAATTGTCCTATCAATGAATTTTTAAAGTTTTTAGAAGCTGAAAGATC ACTTTGAAGCATAAGGACAAGTCACATTTTCCAAAATTTCACCTAAGGGACAACGGTCTCCAGATTTTTT TGCGGGGGAGAGTTGGCTTTTGTATAAATAGGCTTGGGTGAGAAGAGGAGGGGTCCCATTATGACTTGTG CTTTCAGGATTTGTACCAGCTCTTGCTCTAACCTGGTCTGCCTCTCAACAATCTCATATAAGCTCCCAGG AACTGAAATAAGTGGTTGCTCTTCAAGATTTCTCTAGTCTCCCATATATTAGATGGTATGAGAGTGGCTT GGAAAAATTATATGTAATGTTTTCCATGTTCAGCAGATCTCCATTGGTTGCAGGCTAGAGAATGAAAAGG CACTGTTCCTGACCCAGTGCTGCCTCTGGTTGCTGTTCAGTCCTAGAGAAGTCCCTTAGTTTCTTGGTGA TGCTTAGAAAACTGGAAAGTTATCACTTGTAAACACACAGCTGGTAGAAATTTTTCTACTCTGTTGTAAC TGGGCCTTGAAAACAATAGGTTGGTCCCATCCTTCTGGAAGCTCCAAGCCTCCTTCTTCACAATGTGCAA TCTTGAGTGAGTGCTCAACCATTGTCGTCTTTTGCTCAAATGTGAACATATTTAAGGCACGTTTTGCCTT TTTGTCTTGTAACTTAGAATCTCCTAGTCATTCTCAGAAATGTGGCAAGGTGGCTGCAGTGTTTCAGCTT TAGAAAGAAATGTAGTGGCAGAGCAAAGATGAAAGCAAATGGTCCTCATTTTATGAAGCATGAATTCACC TATATTGTCCTACTGGGAAGAGTGAGAAAATAGTTCAATTTGCTGGAGTGGAAGGCAGGGTACCGGGAGA TTGCTTTCATTTCTAACTTTATTATATAATACCAGTAATTGATTACTACCTTTAAATAAGATCCGTGTTT ATTTTTAATCCCATCATGGAAACAATGAGACTGACCTAATTTGGAGGAAATTAAAAATAATGGTTTTCCT CCTTCTAGAACTTGGTGTTTATAAAGTACTGAACCTATACCAATGGTTTTCAAACTTGGGTGGTGCCTAA ATCACCTGGTGATGTTGGTAACCACACAATGGCCCAGGCCTAGTCCGATTTCACCCATCTGGGTGTCAGA GTTCTTCATTAGGTCCTCAGGTTACTCTTTTACGCACCAAACATGAGAGCCATTGGCTATAGTAACTCAT TTGATGCTTACAGCGAAGACTAAGTACTAAGTGATAGCAGTGACGGAGACCCTAGGGATCATCTAAACAC TCTCCTCATACTTAGTGGAGAAAACAGAGATCTGGAGAGCCTCAGTGACTGGTTTAAAGTCAGATGTATT GCACACACAGAATTCACACCCACATTTTTCTGTCACTCTACTTCTGGGGTTTTTTCACTATATAATTTTC AGATCTCTGAAGTGGTAAATTTATGAATATGTGATGTGCTCCATCCTGTTACAGTACAACTATCAATTGA ATTGTTATAATTTTAGTTTTTATCACTGAAGAAACCAACATTACATATTAAGGTTCATTACTGCTAGTGA AAATAATTTGTTTTAAAGAATACTTTGATTTTCTTGGGCCTAGACAGCAGAATATCCTAATGACTCATAG GCCTGAAATTAATTTTGCTGTTTTCTTTCCCAATAGGTGGATGATCTGCAGATCCAGCGCAAAGCCATAA ATGAACTCATCAAAGTGATGAATGACCTGTCACCAAAATCTAACCTCAGAAAGCGGAAGAGAAGTCAGAA TCTCTTTCGAGGCCGGAGAGCATCAATGTAATGGTCCTCCTGCCTGCAATATTTGAATTTTTAAATCTAA ATCTATTTATTAATATTTAATATTTTACATTATTTATATGGGGGATATATATTTAGACATCAAAGTATTT ATAATAGTAACTTTTATGTCATGAAAATGAGTATCTATTAATATATGTGTTATTTATAATTCCTGTATCC TGTGACTATTTCACTTGACCCTTTTTTTTCTGAGTAACTAGGCAAGTCTATGGGATTTCAAGGTTTTATC TCAGGGGCCAACTAGGCAGCTAACCTAAGCAAGAATCTGTGGGTTGTGCACTTATTTCACTTGATGATGT AATGAATGCTGATAAATGAAATGATGCCATCTAGTCACTACTTATCTGAGACTAGATCTGGATTCTGAGC CACTACTTTGATGGCATGTCAGACAGCACTTGAATGTGTCAGGCTATATGACTTGTGCCCTGACAAAAAC ATAGCATCTCATCTCACCTTATACCTGGCGCTTCAGGATATCACTGACAATTGTGATTACACCCAAATGG AAAGTAATATGTTTGTTTAGTTTATCAATATTTAATATATATGAATAAAGTATAATTTCATAAC >gi|31982949|ref|NP_776511.1| interferon gamma precursor [Bos taurus] MKYTSYFLALLLCGLLGFSGSYGQGQFFREIENLKEYFNASSPDVAKGGPLFSEILKNWKDESDKKIIQS QIVSFYFKLFENLKDNQVIQRSMDIIKQDMFQKFLNGSSEKLEDFKKLIQIPVDDLQIQRKAINELIKVM NDLSPKSNLRKRKRSQNLFRGRRAST Vaximmutor Ifng (Interferon gamma) Mouse 15978 33468859 NM_008337 NP_032363.1 MGI:107656; UniProt:P01580 10090 ? >gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA AGTATACCTTTGCAACC >gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus] MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ LLPESSLRKRKRSRC IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria. Vaximmutor The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes [Ref2101:Wang et al., 2009]. IL-1b Mus musculus 16176 6680415 RP23-384K11.2 AL808143 NP_032387 10090 2 129364578 129371119 - interleukin 1 beta 4.31 29045.55 269 Also known as Il-1b; IL-1beta >gi|372099108:129364578-129371119 Mus musculus strain C57BL/6J chromosome 2, GRCm38 C57BL/6J GTTTGTTTGTTTTAATGAAATTTATTTCATACTCATCAAAGCAATGTGCTGGTGCTTCATTCATAAAAAG TCTCATCCATATTATAGGAACCTATTTATTCTCAGCTTCAATGAAAGACCTCAGTGCGGGCTATGACCAA TTCATCCCCCACACGTTGACAGCTAGGTTCTGTTCTAGAGAGTGCTGCCTAATGTCCCCTTGAATCAACT TAAATAGATCAACCAATCAATAAATACATAAATAAATAGGTAAGTGGTTGCCCATCAGAGGCAAGGAGGA AAACACAGGCTCTCTTTGAACAGAATGTGCCATGGTTTCTTGTGACCCTGAGCGACCTGTCTTGGCCGAG GACTAAGGAGTCCCCTGGAGATTGAGCTGTCTGCTCATTCACGAAAAGGGAGCTCCTTAACATGCCCTGG GGAAGGCATTAGAAACAGTCCAGCCCATACTTTAGGAAGACACGGATTCCATGGTGAAGTCAATTATGTC CTGACCACTGTTGTTTCCCAGGAAGACAGGCTTGTGCTCTGCTTGTGAGGTGCTGATGTACCAGTTGGGG AACTCTGCAGACTCAAACTCCACTTTGCTCTTGACTTCTATCTTGTTGAAGACAAACCGTTTTTCCATCT TCTTCTTTGGGTATTGCTTGGGATCCACACTCTGCAGAAAAGTAGAAAAAAGAATTGTGTTAACGTCTGT GTCCGTGTGCATGTTGCATGTATGACAGAAAGGATAAAGCTACCACGGTAGACATATTCTCAGGGCTGCT GGAGAAGAGAATGAAAAAGAGGTTGTGGAACTTGAGGCCACATTCTTTACTGCTGAACCCCATCTTTCTA TAATCACTGAAGGAACCTTTGTTATATATATATTTTTTCTGAATCCTACCATATGGGTGAGAATTATATT AAAGCTCCCAAGGGTACCCAGTTTCCAGGCATTTTTGTCACAGTTCCCCCTAAATCCCTGGATAGAGTGG CATCAGCTTGGAAATGAAGCTTTTGTTAGGAATGTCACAAGTGCCAGTACAGGTCTGAGGATCGTTGGTT GAACCCTTTCACTGCATTCTCCAAACTGTTCTCTGTCAAAGGAGGAAAAACTGTCACCATATACAACTCT TTCATCCTTGATGCTACGACTTCTGTTGGGGGTGATAACGGTGGCCTGACATGACAACAGCATCAAAAAG GATTGATTTCCCATGGTGGCAGTGAGGTTTATGTGCACTTAAAAGTTATGCTAATTTTATTCTATATTTA TGTAAATCGTCAAGAAGACCTCTAATGTTTTCTGGCGGGTCAGGCAGGTGGATGTCTGGGAAGTAAGTAA AGGCAGAGTCTTCGGTGAGCAGTGGGGGAGGCTTCTCTACTGATGGACTCACCTCCAGCTGCAGGGTGGG TGTGCCGTCTTTCATTACACAGGACAGGTATAGATTCTTTCCTTTGAGGCCCAAGGCCACAGGTATTTTG TCGTTGCTTGGTTCTCCTTGTACAAAGCTCATGGAGAATATCACTGGAGAAAGGAGAGGAGTCTGGGTCA AGGGAACAGCAATGCAGGGTGGGCTCAAGCATTAGACGTGACAGGCAAAGCCTATGCAGTCCCCCGTGTG AACCCAGAATGAGCCTCCTCAGACACAAGATATGGACTAGCAGGGCTATACAGTCATAGTCATAGACAGT AGCAAGGTCAGTAGGCCGATCCAGGATTCCCTCAGGACCTCTTAGGAGCAGCAATGAAGAACGAAAGGTG TCTAACTGTTAAGCTCTAAGGGCTAAAGCTGTTATGTGGCTGGCAGGCTGTGTGTCCGTCTACGTGAGAA GTACAGTTTGGTCGCTATGAAAACAAAGCTGTGCTCCTAAAAGGACCTAGGGGCCAGCACGTTTTGAGGA AAATAGAGCAGGAGGCAGGTAGAACTCTATTAGCTGGGGAGAGTGGCTCCAAAAAAGAGACCCCCCCCAC TTTGATCTTAGCCACAGTACCTCAATTGGAGGCCCCTGGCTGCTTTTATGACTTTATCAAGAAATAAGAA TTATTCTTTTTAAAAGGTATAAAGCAATAAGATTCTTTTCACCTGGACCAGAGTAGACAGCTCCCTCCAA AGCTTCTAAGATCAGAAGCTAAAAGGAGACAGCACACACTGCTGCTGTTAGCTCTTGTCCCCTCAGCTGT GGTGGTGTGGTGCCAGGTCCACACCATCATCTAAAGAGGCCCTGACCGCGCTTGGAATGCTTGTTATTAT GGAAGAGAGATGTATGCTCCTGATAGTCAGCAAACCTCCCTTCAAGGAACTGTGTGTGTGGTATTGGTGG GAAGGATATCAGCACGAGGGATAGGACTGGGAACAAAAATGGACATATCAATTCTCTACCATCTACATTT CAGCCTCAAATGAGGGAAAAAGAACCCACAGGCAGTTATTGCATGTCCATCAAATGACTTCACACTAATG TGTTATCCCTATATGACTGGGCTGGAAAAATGGTCCTTTTTCGTTCTGTCTCTGTCTCTGTCTCTGTCTC TCTCTCCTTCCTTCCTTCTTTCCTTTCCCTTTCTTTTTCCTTTTCTTTTTTTGTATAATGACCATAGTGT TCCATTTTAAAACACAGGGACCCAGTAGGCTGCCAGGAGGCCAGAAAGGGAAACCAAGATGCTCCTACTT ACCTTGTTGGTTGATATTCTGTCCATTGAGGTGGAGAGCTTTCAGCTCATATGGGTCCGACAGCACGAGG CTTTTTTGTTGTTCATCTCGGAGCCTGTAGTGCAGTTGTCTAATGGGAACGTCACACACCAGCAGGTTAT CATCATCATCCCATGAGTCACAGAGGATGGGCTCTGAAAGTTCAAGAGCAGATGTTGTTTAGGCAGATAA TTAGAAATGCAAGCACTGTGAAGTGAAGCAGCAGAATTTGGCTTATTGAGCAACACCTAAGCATGTGCAG TCAAGATTCTATTCTAATTTTAGTGGGGGTATAAAAACACTGGGAGTGGGCTGGGCAAGAACATTGGATT TGGCATCGTGAGATAAGCTGCAGTGTTATAACCATTCACTAGGGACTAGCAGTAGTGGTCCTTGCATGAA ACGATGCCAGTAATAAGACCAGCTTAACTGGGAAATTGTGAGATTCACAACCTCTAGTATAAGCCAAAGT ACTTGGCAAGTCAATGGTTGAGTTGCAAGTGTAAGCATCATTAAAGCCTTTTTATAAGTTAATCAGCTCA AGATAGAACTGATTTGTTTGGAGGTTTCCTCATTTTCTTCTAATGGTTGGAATGTCTAGCACTAAGGGTC TTGCACTTGAGGGGCGTTGAGTTCTAAGAGGTGGGCTGAGAAGGACCAGAGCGAAGGTCTGCTGTCTGCT CTCAGTCCTCATTAAGTACCTTCTTCAAAGATGAAGGAAAAGAAGGTGCTCATGTCCTCATCCTGGAAGG TCCACGGGAAAGACACAGGTAGCTGCCACAGCTTCTCCACAGCCACAATGAGTGATACTGCCTGCCTGAA GCTCTTGTTGATGTGCTGCTGCGAGATTTGAAGCTGGATGCTCTCATCAGGACAGCCCAGGTCAAAGGTT TGGAAGCAGCCCTGTGGAGAAACCAGGCAGTGAGCACATCAAGTCATCAGACCAGAGGACTCCTACATCA ACCCCAGAGAACACCAAGGGCCCGGGAGATAAGTTCTTAACCACAATCCCTGTCTATGTGGAGCCCAGAG AATTTAATTGGCCTACCCAGGGGTACATGGCAACCAGACACAGGCTTCCTGGCATTCTCTATAGTAAACC TCATTTCTACTTTATCTTTCTTGTACAGACCTTGCCTCACTGTATAATTAGACCTACTTAAACGTTTCAT CTTTTGGTATCCTTGCTGCTCAAACATCATGTTATTTAATTTCTCATGACAGTATGTTCATGTTGTCTTC TATTCAACGGAGATCACCCAACAGGCTCTCAATGGCTTCCCATACATTCTTTTTCTCATAGGAATATTTA GCAGACACTATAAGCATAGCTTTTGTTTGTAAAATATGTCACCACTTTTCTGTTCTTTGGAGAAACACCA CATTAAGGCCTAAACACAGTTGCCTTGAGGCATGATAACAGTTTCAAAGCTTGAAGGACAGCGATAGATC ATTTCAATAGTGTAAAGGAAAGGTTGATTGGGAGAGAAGTTTCTGTCCAGAGTAGAGGCTCCCAGGGAAA GCCATGTTCTGGAGCAGGCAGTGGGGGACAGAGCCTGGCTGGGAAAGTGAGAGGCGTCACCCAAGGCTGA ACTAACTAGTTAGAGTGGTCTTAAAATACATTCATGTAAGCTAAGGACTAGATTCTGTAGCTGGGGGAGA CTGTTGAGGGATTGGGAAGAGGTCTGAGAGAAACAGCTGCCTCCTGAGTGGGGCAGTGACGTGGCTGGAC TGACGCCATGTGGCATTAGAGCGTTCTCAGTCATCATAGGAAGGGACAGCTGGGATGCAACATGGCTCTT CATGAGAGCAAGTCTGGAAAGTGCCCTTGTGCTAGGATGGACTTGGCCAGCACTTAAAACACTTCAGAAT GGAAGGTTGTGACAGATCGCCAACATTTCAGAGATCTTCTGACCTGTGTTTCCCAAAGGACACAGTGGGG ACTGTCTATGATCACACAGCAATGAAAGGTCTTGTCTAAGGCCACAGGTTATTTTTAAAAATCCTAACTA ACTTTTTGTTTATATCACTTATTTCTTATTATCTTAATCTCCCCAGGAACATGCGAAACTGTTCTTTGGC AGAGAAGGAAGAGATGCTAATTCTGAAGGTCATGCTCTCTAGATCCAGAGTTCGATTTAAGTGGTCAGAT TAAGCTCCATCTCTCTCAAGGGGGGACTAAATGTATGTTGAAGACCATACTGTCTTCTTAGCATAAAACA CCATGATTGCCATCAGCAAGCCTTGGTGTGTGGCTGTGGTAAATGAAACCTGTGTGGGAGCCAGGTCAGT GTGTGGGTTGCCTTATCTATGTCACTGAAAGAGGCAAAATAATCCCATCACAAAATAGCCTGGGAGCAAG GCAGTCCCATATCCCTTCCCGTTTGGGTTTTTTTTTCCCATGCCACAGTCCCTCCACAGGTTAGGAGCCA AGCTCAGAGTCTCACCTTCATCTTTTGGGGTCCGTCAACTTCAAAGAACAGGTCATTCTCATCACTGTAA TGAAAACAGATGTGGTCAAGTGATAGCTCCTTATCAATACTGAGCAGACAGACTTCCATTGTTCATAAAA CCACACCTACCATGACTCTTAAAGCTTCCCAAAGACTTCTGGGAAGTTCTACAGTGCTACGTAAGTAACA GTCTAGAAGCAGATTCTGGGCCAGGTAAGTGGGTGCTCTGGTTGCTCTCTGTTGACTTCTGACACCTAAA TTCTAGACTAGCAGAGTTAGCTAAAGTCTGAGCTCTCTGCCTTTGCTTCCAAGCCAGACTTTGCAAAAGA GTCTCTTATCCTGTCCATGTGGAAGTTTTTCTTACCATTGCATCTGTAGGAGTCCCTGTAGTGACAGCAC CTAAGTCCCTAGGTTGGGCTTGGGAGTGAAGAGGTCAGTCTTTAAGCTAGGTTGCTTGAACTCTGATAGC CACTGAAGGAGATGAGTTGGGGACTCTCTGGCCTCTGAAAGTAGATAGCCCTCCATTCCTGAAAGCAGAG TGCTGGATATGTGACCACTCTCCAGTACCCACTGACCTGTCAAAAGGTGGCATTTCACAGTTGAGTTCAG GAACAGTTGCCATAGCTGCTTCAGACACCTGTGTGAAGAAAGAAAGTGGGTGGGCATCCAGCGTTAGCTC CCCGAGGGGCGTCCTTCATATGTGTTTCTCTTAGCTCTAGTTCAGGGCAGGCATGCTAAACTGGTCTCTG AGGAATGAAGGAACGGAGGAGCCGTTGTATAGGTCTAGAACAGTGGCCCTTTTGGAGTCAAGTGGCCCTT TCACAGGGGTCACCTAAGACCATTGGATAACACAGATATTTACACTTCATAACAGTAGCAAAATTATAGT TATGAAGTAGCAATGAAAATAATTTTAGGGTGTGTGGGGGGTCACTACAACATGGGGAACAGCATTAGGA AGATGCTCTGGAAGGAAGCAACAGCAGAGCCAAACCCTAATAAAGAAGCTTTCCTACAAGACAGGAGCTG CCTTTTAGATGTCAGCGGCTATACAGACAGTACTCATTTCCAGAGTACTTCACAAACCATGGAGGTACAG CTACTTTTTATAAATAAAAAGCCCAAAGTCCATCAGTGGGGGGGGGGGAAAGAGAGAGAGAGAGAGAGAC AGAGAGAGACAGAGAGAGAGACAGAGAGACAGAGAGAGACAGACAGAGAGAGACAGAGACAGACAGAGAG AGAGAGAGATAGACAGACAGAGACAGAGAGAGAGACAGACAGAGACAGAGAGAGAGAGACAGAGAGAGAG AGATAGAGAGAGACAGAGAGAGAGAGAGACAGAGAGAGAGAGAGACTTACTTGCACAAGGAAGCTTGGCT GGAGAGGATCCCAGATGAGCCTATTAGGCCTC >gi|6680415|ref|NP_032387.1| interleukin-1 beta precursor [Mus musculus] MATVPELNCEMPPFDSDENDLFFEVDGPQKMKGCFQTFDLGCPDESIQLQISQQHINKSFRQAVSLIVAV EKLWQLPVSFPWTFQDEDMSTFFSFIFEEEPILCDSWDDDDNLLVCDVPIRQLHYRLRDEQQKSLVLSDP YELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKK KMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS Vaximmutor IL-6 Mus musculus 16193 13624311 AC112933 NP_112445 10090 5 30013160 30019967 + interleukin 6 7.5 23291.86 211 Also known as Il-6 >gi|372099105:30013160-30019967 Mus musculus strain C57BL/6J chromosome 5, GRCm38 C57BL/6J ACCAAGAACGATAGTCAATTCCAGAAACCGCTATGAAGTTCCTCTCTGCAAGTAAGTGAAGGCAGTTCCT TGCCCTCTGGCGGAGCTATTGAGACTGTGAGAGAGGAGTGTGAGGCAGAGAGCCAGCATTGTGGGTTGGC CAGCAGCCATCAGCTAGCAGCAGGCGCCCAACTGTGCTATCTGCTCACTTGCCGGTTTTCCCTTTTCTCC ACGCAGGAGACTTCCATCCAGTTGCCTTCTTGGGACTGATGCTGGTGACAACCACGGCCTTCCCTACTTC ACAAGTCCGGAGAGGAGACTTCACAGAGGATACCACTCCCAACAGACCTGTCTATACCACTTCACAAGTC GGAGGCTTAATTACACATGTTCTCTGGGAAATCGTGGAAATGAGAAAAGAGGTGGGTAGGCTGTGAAACT GATGAAGACCCAGTGTGGGCGTCCATTCATTCTCTTTGCTCTTGAATTAGAAATTCTCTGCTGGGATCTA GGGCCCTTAGGATTTGAAGCTAAAGGTCAGACTAGACTGTGTTCTCTCTTTCTCACCTCTTTGCTGGTTT TGAGTGGAGGTTGGGAAGGGGTTCCTTTCCTGTCTGGAAGATACAGAATGTGACTGCATTTCTAGAAAAT TCACAGTGGGCCATTCTCTGTTCCAATTTAGCTATTGCTTCTTAGGTGGGGATTCCTTTTCCCTCATTAT TCCTTTTTCCTTATCTCTTTGCCTTCATTTATCCCTTGAAGTCTACAAGGACCAGAAAGTGCTTTTTGGC TGAATGTAGTAAGTTCTGTGTGTGTGAAATCAGGATGCTCTAGGGTCAGCCCAGATAAGATGCATATTGT ATAGAGCCCAATAAAGTGCTAACACACACACACACACACACACACACACACAACCTAGATTAGGCTGGGC ATGGTGGTGGATGCCTCCCAGCACTTGGGAGGCACACGCAGGAAGATCTCTGTGAGTTAGAGGCCAGCTT GGTCTACATATTGAGTTCCAGGATATCCAAGACTACATTGAGAGATCCTTTCTCAAAAAAAAAAAAAAAA AAAAAAAAACAACAACAACAAACAAACAAACAAACAAGAAAAAAAAAACCCAACCAACCCTACCTAGATT AAAATATCTCCCATCTTTAAATGAAAAGTATTTTGACTCTGCTTGACAGAAAGGAAAAAAAAAATCACAA ACAAGATTAATAGCCTAGGTCTGGGTGTGTAGAACCAACTTTGTAATCTTGGGAAAATCCTCAACTATTT AGTACTCTTTATCTGCATAATTATGGAGTTACAGATAATGGCACAGACCCTTCCAGATGGAATATTCTGT GACTTAAATTTATTTGCCCCAAGATATCATCAAAATTTCTGTCCTTTACCAAGTTCCTACTCTATGCTGG GCAGTTTTTATCGTTGAATCCTTGAGACAAATGTATAAGAGGGACATCAATAATTCTTTATTGAAACATA GGAAATACTTAGGAGAGTAACAAGCTTAAAGCCAAGGGACTGAGCCTAAGGGTGCATTTTTATTTATTGG GCTGCTTAGTAGGGTTCTAAGAAGGTATAGCTTCCCTTTTCTGTGTCCTGGCTATGAAAGAGCATGACTT GGCCCTCTAGTGGTGCTTGTTGTAAGAGGTGTTTAAGTGATTGCTCTGGTAATCTTTTGCAGTTGTGCAA TGGCAATTCTGATTGTATGAACAACGATGATGCACTTGCAGAAAACAATCTGAAACTTCCAGAGATACAA AGAAATGATGGATGCTACCAAACTGGATATAATCAGGTAGAAACTTGTCACGTTTACTTTCAACAATTCT TCAAGGTTCTCTCTTGCACGTGATACCTGCATTTGTACAGTCCCAGTCAGGCAACAAAGATGGGGGGATA CATCCAATACCACTTGGATGTAACTGGCCTGCAGTAGCTTGAATACAAAAGGAGATTGAGAGGTACCTCA GCAGCTGCTAGGGTGAGGGAAAGTTTACCAACCAGTGCTATAAATGTTCACTCAATCTATGTCCATCAGG AGCTAAATGTTTAAAGTCTCTATTCTCATCTTGGCTTTGAGGTGTTAGATGATTAAAAAAAAAACATTTT TTTTGTCTAAAAAAGTGGAAGAGTAGATATAAAACATGTTATGTAAACGTCATGAGGAAAGTAACTTACA GATTTAATTAAAAAGCAGTTTATTTAAACAGGTAAAGGCCCACTATGCTGGGATTCTTCACCACTGTTAA ATGTTAGAAGTCATAGCAAGTTTAGCTAGTTCATGAGCCATCTCTTCTCTGGCTGCCCCTGGAAGGATCC AGGCCTACACTCCCTACTGAGAACACTCTTCACACCCCTCTCCTTCCAGCCGGTGAGATAGTCTACATCA GGTCTCAGGACAAGTCAGACAAGAACAAGAGTACAGGAAGGGCACTGCAGGATAGAAGGGAAGGAGTGAG GGTCAGAAGTGGATTCCCACTGCCACACTCTGGGGTAGGGGGTGGGGGGATATTAAGTGGTGCCTCTCTC TACTTGTCCAGATAATTCAGGAGGTCAGCTGATGCTGCCTGTTGTCTAATTCTGTAGAAGATACTGAACA GCAGGAATCTTTTTTTCAATTTTTTATTAGATATTTTCTTCATTTACATTTCAAATGCTATCCCCACAGT CCCCTATACCCTCCCCCCCCTCCCCGCTCCCTTACCCACCAACTCCCACTTCTTGGCCCTGGCATTCCCC TGTACTGGGGCATATAAAGTTTGCAAGACCAAGGGGCCTCTATTCCCAATGATGGCTGACTAGGCCATCT TCTGCTACATATGCAGCTAGAGATATGAGCTCTGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTAT AGGGTTGCAGACCCCTTCAGCTCCTTGGGTACTTTCTCTAGCTCCTCCATTGGGGGCCCTGTGTTCCATC CAATAGAAGACTGTGAGCATCCACTTTTTGCCAGGCATTGGCCTAGCCTCACACGAGACAGCTATATCAG GGTCCTTTCAGCAAAATCTTGCTGGTATATGCAATACTGTCTGGGTTTGGTGGCTGATTATGGGATGGAT CCCTAGGTGGGGTAGTCTCTGGATCTTCCATCCTTTTGTCTTAGCTCCAAACTTTGTCTCTGTAACTCCT TCCATGGGTATTTTGTTCCCCATTCTAAGGAGGAATGAAGTATCCACATGTTGGTCTTCCTTCTTCTTGA TTTTCTTGTGTTTTGGAAATTGTACCTTGGGTATTCTAAGTTTCTGGGCTAATATCCACTTATCAGTGAG TGCATATCAAGTGACTTCTTTTGTGATTGGGTTACCTCACTAAGGATCATACTCTCCAGATACATCCATT TGACCAAGAATTTCATAAATCCATTGTTTTTAATAGCTGAATAGTACTCCATTTGTAAATGTACCACATT TTCTGTATCCATTCCTCTGTTGAGGGACATCTGGGTTCTTTCCAGCTTCTGGCTATTATGAATAAGGCTG CTATGAACATAGTGGAGCATATGTCCTTATTACCAGTTGGAACATCTTCTGCGTATATGCCCAGAAGAGG TATTGCTGGATCTTCCGGTAGTACTATGTCTAATTTTTTGAGGAACAGCCAGACTGATTTCCAGAGTGGT TATACAAGCTTGCAATCCCAACAGCAATGGAGGAGTGTTCCTCTTTCTCCACATCCTTACCAGCATCTGC TGTCACCTGAATTTTTGATCTTAGCCATTCTGACTGGTGTGAGGTGGAATCTCAGGGTTGTTTTGATTTG CATTTCCCTGATGATTAAGGATGTTGAACATTTTTTCAAGTGCTTCTCAGCCATTCAGTATTCCTCAGTT GAGAATTCTTTGTTTAGCTTTGTACCCCATTTTTAATGGGGTTATTTGAATTTCTGGAGTTCAGCTTCTT GAGCTCTTTGTATATATTGGATATTAGTCCCCTATCAGATTTAGGATTGGTAAAAATCCTTTCCCAATCT GTTGGTGGCCTTTTTGTCTTATTGACAGTGTCTTTTCCCTTACAGAAGCTTTGCAATTTTATGAGGTCCC ATTTGTCGATTCTCGATTTTACTGTACAAGCCATTGCTGTTCTGTTCAGGAATTTTTCCCCTGTTCCCAT ATCTACGAGGGTTTTTTTCCCACTTTCTCCTCTATAAATTTCAGTGTCTCTGGTTTTATGTGGAGTTCTT TGATCCACTTAGACTTGAGCTTTGTACAAGGAGATAAGAATGGATCAATTCGGGCTGGAGAGATGGCTCA GTGGTTAAGAGCACTGACTGCTCTTTCAGAAGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGTTCAT AACCATCTATAATAAGATCTGATGTCCTTTTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACATGTA ATAAATAAATAAAACTAGATTATTTAAAAAAAAAAAAAAAGAATGGATCAATTCACACTCTTCTACATGA TAACCACCAGTTGAGCCCGCACCAATTGTTGAAAATGCTCTCTTTTTCCCACTGGATGGTTTTAGCTCCC TTGTCAAAGATCAAGTGACCATAAGTGTGTGGGTTCATTTCTGTGTCTTCAATTGTGTTCCATTGATCTA CCTGTCTGTTGCTGTATCAATACCATGCAGTTTTTTTTATCACAATTGCTCTGTAGTACAGCTTGAGGTC AGGCATGGTGAGTCCACCAGAGGTTCTTTTATTGTTGAGAATAGTTTTTGCTATCCTAGTTTTTTTTTTT TTTTTTTTTTGAACAGCAGGAATCTTATTCTCAAATTGAATCTATTCCTAGAAAGAACTGACTTCCTTTT CCATTTTACTTATAGGAAATTTGCCTATTGAAAATTTCCTCTGGTCTTCTGGAGTACCATAGCTACCTGG AGTACATGAAGAACAACTTAAAAGATAACAAGAAAGACAAAGCCAGAGTCCTTCAGAGAGATACAGAAAC TCTAATTCATATCTTCAACCAAGAGGTGAGTGCTTCCCCATCTCTCATGCAGTGTGGGAAAGAGGACACC CGGCACCTCAGGGTAGCGGCACTTTTTCCAGACAGCTGCTCAGAAGGGAGAGAGTCTGAACAACAGTTCT TGATTATAAGGCCTTGCTTTGTTTTACTTTGGGGTTTTGTTTGAGGTTCTCTTTTGCAAAGAACATCAAT ACCTGCTTTAAACTGTATTAATAGAATGTTACTAATTGCGTAAGAGGTATGAAAACTATGACAGCCATAC ATAGTCACCCATTATGAGAGCACAGAGACAAAAGTGACTTTAATATTTAATCCTTGGCAAGTGACATTTT TGTAACCAGAGTTCTAATGCAGAGAAGTAGCCAAAAGCTAAAATGTCAGGGAAGGAAGGGATCAAGGGCT TCTGGCTACCATTAGCCAGAAGAATGGTAGATATGGAGGATTAATGACTCAGAGTGTGGGCGAACAAAGC CAGATGCAATAAGAAGGGCCTGGAATGAAACCCTCTTGCTAAGGCTGCTTTTGCCACTTGTAGTTTCTTG CCTTAAACCAGAGAGTTTCCCAATTTAATGTGCACAGGAACCACTTAGGGTCTTGTTCCAGCAGGGTCTT ACTTAGGAGGTCTAGGAAGGGAACTAAGATTCATTTTTGTAAACAGCTGCTTGTTTATGACCCTGCTATA TAGTATTCAGGCTCCTAAGTAGTGGGGCAAGCCTTCCAGTTAGTCTTCCCCATGCCAGTGGGCCCATGGA AGGGTGTTTCCAGACTTCTTCATGCTACCACACTAAGAGACTCTCAACAGAGTCTGAATGGAAACCACGA AGGAACACATTTGTTTTAGATTCCTCTGTGCCACCTTTACTGATGGGAGCTTCTGTTTTCCAGTAGAATA CAGTATGTTAGCATGGATGCTTGGATAACAGACAAATAAGATGGTGGTGCTGACCTCTGGACGCTTACTC TCTAGTGGCAGACAGAACAGTAAGGTTAGAATTCTGTTGCTATTAAAAAACTAATAATAATCACCTTGAA AAAGAATGGAGTTGTTAGGCATGGGTCTCTCTCGAGTAAGCTTGGAACAAAAGCATCTCCCCTGGCTTGG GTGAGTCAAAGCAGATGGACTTAGCTCGTCTCATTCATTCTAAATTAGAACTTCTTCCCACAGCCCAGAA CACGCCACAAGAAAAAAAAAATGTGCAATATTTAACCAGTCTTTGTTTTTTCCTCCTTTAGGTAAAAGAT TTACATAAAATAGTCCTTCCTACCCCAATTTCCAATGCTCTCCTAACAGATAAGCTGGAGTCACAGAAGG AGTGGCTAAGGACCAAGACCATCCAATTCATCTTGAAATCACTTGAAGAATTTCTAAAAGTCACTTTGAG ATCTACTCGGCAAACCTAGTGCGTTATGCCTAAGCATATCAGTTTGTGGACATTCCTCACTGTGGTCAGA AAATATATCCTGTTGTCAGGTATCTGACTTATGTTGTTCTCTACGAAGAACTGACAATATGAATGTTGGG ACACTATTTTAATTATTTTTAATTTATTGATAATTTAAATAAGTAAACTTTAAGTTAATTTATGATTGAT ATTTATTATTTTTATGAAGTGTCACTTGAAATGTTATATGTTATAGTTTTGAAATGATAACCTAAAAATC TATTTGATATAAATATTCTGTTACCTAGCCAGATGGTTTCTTGGAATGTATAAGTTTACCTCAATGAATT GCTAATTTAAATATGTTTTTAAAGAAATCTTTGTGATGTATTTTTATAATGTTTAGACTGTCTTCAAACA AATAAATTATATTATATT >gi|13624311|ref|NP_112445.1| interleukin-6 precursor [Mus musculus] MKFLSARDFHPVAFLGLMLVTTTAFPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELC NGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKAR VLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQ T Vaximmutor IL10 Homo sapiens 3586 10835141 RP11-262N9.1 AF295024 NP_000563 9606 1 206940947 206945838 - interleukin 10 8.12 19821.37 178 Also known as CSIF; TGIF; GVHDS; IL-10; IL10A >gi|224589800:206940947-206945838 Homo sapiens chromosome 1, GRCh37.p5 Primary Assembly TGATGTGAATAAGATACATTTATTTATTCAAAATTAAAATCTGCTATGAAGACAGACAAACAATGTAACA TTCCCAGAGGAATTGAATAAATTCTAGTTAAAACTGAGTTCTATTAGAACCAAATTTATTTTTATTTTTA TTTTTTGAGACAGAGTCTTGCTCTGTCACCCAGGCTGGAGTACAGGGGCATGATATCAGCTCACTGCAAC TTCCATCTCCTGGGTTCAAGCAATTCTCTTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGTGCGCGCC ACCATGCCCGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGACCAGGCTGGTTAGGA ACTCCTGACCTCAAGTGATCCACCCGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGC GCCCGGCCTAGAACCAAATTTAGGTTGTTATAAATAACAAGCTGGCCACAGCTTTCAAGAATGAAGTGGT TGGGGAATGAGGTTAGGGGAATCCCTCCGAGACACTGGAAGGTGAATTAATCATCAAAGGGGCTCCCTGG TTTCTCTTCCTAAGAGTATTTGTAGCAGTTAGGAAGCCCCAAGCCCAGAGACAAGATAAATTAGAGGGAG GTCAGGGAAAACAGCTCAACAGCTAGAAAGCGTGGTCAGGCTTGGAATGGAAGCTTCTGTTGGCTCCCCA AAGAACATTTTTTTTCCTCCCTTATGTAACTTATAATTTATCTTAAAACACTCAAATACCATAGTGTGTC ACCCTATGGAAACAGCTTAAAAACAGGTGAAAATAATAAATATTGAAAAAAATTATAATATTGGGCTTCT TTCTAAATCGTTCACAGAGAAGCTCAGTAAATAAATAGAAATGGGGGTTGAGGTATCAGAGGTAATAAAT ATTCTATAAGAGAGGTACAATAAGGTTTCTCAAGGGGCTGGGTCAGCTATCCCAGAGCCCCAGATCCGAT TTTGGAGACCTCTAATTTATGTCCTAGAGTCTATAGAGTCGCCACCCTGATGTCTCAGTTTCGTATCTTC ATTGTCATGTAGGCTTCTATGTAGTTGATGAAGATGTCAAACTCACTCATGGCTTTGTAGATGCCTTTCT CTTGGAGCTGTGCAGAGAGATAAGAAACATACAGTTAAAATCCTTTCTGGGGACTAAATAGGCTCCCCTC CCTCATGCTCATGGATGGGCATGACCTTGAGTGCAGAGGTTGCTTGTTCTCCCTCACGCTGGGAAGTAAA CCAGAGGATAGGCCTAGCTGCCTTGGGAAAGGCTGTGCATTGACCTTCATCTCCTCCAGGCCTCCCCCTA GCAAGGGGAGCATTCATTTTGCAAAGCACTGTCTTCAAAATGATGCCTTTTCAACAATTTTAAAGGTCAC GTATTCACATGATGATTATGCAGAGTTTGATGAAAAGACATTAGAGGAAGCGCTTCGAAAGCAAGACGGT GAGAGGAGAGGAGGGAGCCTTTTAAATGTCTGTGTTCTCCCACCAACACACTGAGCAGGCCCTGCCCTGC CGCCCTGACCTACTATGAGAAATTCACACCCCCTGATGTTGGCACTCTGTGCAGTGTCTGTCCCCAGCCC ACGAGGAGGACATAGGCCGCACGGTTTCTGGGAAATCAGACTGAAAAAGTTCACGCACAGTTGGAAGTGT GAAACCCCATTTCGTTTGGGAACTGACATTCTCAGGCACCTCCGCAGGTGCGAAGGGCAGATGGAAAGCG GGCTTGAACCAACTCCAGGTTAAGTGAGAATCCAGCTTCCTGCTCATTCTGAGTTCTGGGGAGTCTGGGC TGAACCCCCAGGTGTATTTGTATGTGCACATTTATGTGCCAGCTTCCTGCACGTGTGGGTTCAGCCTAGA TTCCCAACAAGCTACTTTCTGTACTTTCTGTTTCCTTTGTCACTCTCCCCAGCATGCAGCTCACTGTGTT CACAGGCTGGCCGGCCAGCCTAACCCGCAAGCCTGCAAAGGCAGCGAGCAGTCATTTAGAAACCCCCAAA GACACTTAACAGAAAACAAATACAATCAAGGCAGCAGCTCCTCACTGGCTGAGCAGGTCATACCATCTGT CAGGTTCCCACACTCTCTCCAATGAAGAATGGGGCCTATTGAGTCCCCACCACCTTCCATGCTTTGGGGT TGGGGAGTGGGCATGGGTTGTGCTCTGCAGACCCTCTCTGCCACCATCCAAGCTCACCTTATTAAAGGCA TTCTTCACCTGCTCCACGGCCTTGCTCTTGTTTTCACAGGGAAGAAATCGATGCTGTGGAAGAAAAGAGA AAGTGTTGGTGATCCTGGCTTCCAGCTCCATGTCCATCACACTTAGGGGACAAGCTGGTGGCATCATGAG GAGGCCAGATTTATCCAAATGCCTTGCCTCACAGCTCCCAGAGAGAACTGAGCCCTGCTTCCATGGCCCT GGGAGTTAAATGTATTTCTCCAGGACTCACTCCAAGATGTTTGCAAGGCTTGGATGACAACCCAGGTCTT GGGATTCCTTCCTCCCCCAGAACTCTACAGTATCTCCTGCAGTGCTGAGCGAGGCATTCTTCAGCCTGTC CTTTTCTGCCTCTCTCTCCTGTTCCTCCCCGTCAGTCACAGCCACCGAGGAACACTTCTAAGCATCTGAG GAGGGAAGCTACTGGGACCAAACCCTTCACATAGCCTTGTCCTACAAATAGACACCAACTGATTTTCCAA TATTGGTGACAGAACAGGGGAAACCATTAGTAGTGAGGGCATCATTTCAGTTGGAGAAATAAATTACCTG GAGGAATTACTCATAGACACTAGCCTGGGGATGAATAGCCCCCTTGTCCCTTCTAAAATGCCAGGGGAAA TCCTGTCAGTCAATTGGGCCCTTCTTAGAGCTTTGCTTTCAAAACATTGAGATTAAAAGAGGTGAATGAT GTTTCCTCTGCTTGGGAACCTTAGCTCCCCGCCCAGGGTCTAGTTACAAAGAGGCAGTGGAGGCCTGACT GAAGCTCTGGGCTCCTTTTATGAAGAGAGAGGGGAGTAGTTAATAACCCACAAATGACTCACAAATAACA GGAAAGCTGTTTGCAAATTTCACATGTAAATGCCTAGCAGCCACCCCCAACGCCTGCTCAAAGAGAAATG AGCAAGAGATCTGACTCCAGGAGTCTTTCCTCATTTACAGCTAGCTCTGCCAGTCTGTGTCTTTGCTGTG TCTGTGGATGTGAGTGTCCCTGCTGGTCTGTAGGAGATGGTATTTTGGGGGCAGCTGCAAGGGAAAAAAC TGATCTGCTACTTACACAGCGCCGTAGCCTCAGCCTGAGGGTCTTCAGGTTCTCCCCCAGGGAGTTCACA TGCGCCTTGATGTCTGGGTCTTGGTTCTCAGCTTGGGGCATCACCTCCTCCAGGTAAAACTGGATCATCT CAGACAAGGCTTGGCAACCCAGGTAACCCTAAGGGCAGGAGCCAAAGGTGAGTGAGAGATTGGCGGAGGT GGCTGGGAGGAGGGGCTGCTGCAACTAGCTTAAGAGGACAGCTTGGTTCTAGCGATCCTCCTTCACCAGA AGCCTCCCCGAAGGGACTGAGCCCTTTGTAAACCCTCTGGCTGCTGGATGTGCTGAGTTAACATCTTCCC ACTTCTCCTTTTCAAAGCGAAGGAAACAAACCCAATTCCCTGCAATCAGGAAGCAGAGTCTCCCTTCCCT TAATCATGCTGCACACTCCCCCAGCACCCCGCCCCTGCTCTCACCTTAAAGTCCTCCAGCAAGGACTCCT TTAACAACAAGTTGTCCAGCTGATCCTTCATTTGCTGCAGGAAGAACAAAAGGAGAATGAACTTGAGGTT TGGGGAAATAACTGAAATGCGGTCTTTTTGATGCCCTTTCATTTAGAGATTTTTTTTTTTAAATAAAATT GGCTCTGGCCCAAAAAAATCAAAAGGGGAGTTTTAAAAACAGAGGATTCAACAGTGATGGGACCATCACT TAAATCAGGTCCTCCTCCTCAGAGTCAAGTTATTTAAAAAATCTGGCCGATTTTGAAGGGAAAGCAGAAG CTTGACTCAGTCCTGGTCTTCTTTCTGCGGAGCTACATTCGGCTTTAAGGGACACCTATGTCAACCCTTC GGGGCCTTGAGCCCCCCTCATGTCCTGTTCTCTGCTTTTCTTTCTCCCCACTGTAGACATCCAGTTTAGC TTGGAAAGATCCCAGGGATTAAGAAGCTCCTTCTAATGCAGGTTTCCCTCATGTAGAGTGCTTCCCTAAA CCACTGGTCTTTCAGGTCTGGAAACGCTCTAAGCAGAGGCAATTTAAATTTCCCTGCTGCAAGGCATGGG GAGCATCTTCACCTCGAATCAGACATTTCTTTACTTCTCCTAGCCAATAAAGTATAGAGCGCCAGCAGGA TCTTATAAGTTTCTCAGCACGAGAGAGAACGCATTTACTTTATAAGGCAGGTTTCCTGCACATTTACTGT ATCATCTGGCTTAGCTTAACTCACCAAACCCATGGCTTGATCTAGGATTCTCTTAAGAATTTAAGTTTGG GGGAATAGGTGTTGGGGATGGAGGTGGAGGCGCAGGAGGAGGGTTCTTATAGTTCCAGGAGAATGTCTAG TTCAGGCAGTCCCAGGAAGAGAGAAAGGACAGGAAGGAATCATACTCACAAAGAAAGTCTTCACTCTGCT GAAGGCATCTCGGAGATCTCGAAGCATGTTAGGCAGGTTGCCTGGGAAGTGGGTGCAGCTGTTCTCAGAC TGGGTGCCCTGGCCTGGGCTGGCCCTCACCCCAGTCAGGAGGACCAGGCAACAGAGCAGTGCTGAGCTGT GCATGCCTTCTTTTGCAAGTCTGTCTTGTGGTTTGGTTTTGCAAGAGCAAGCCCCTGATGTG >gi|10835141|ref|NP_000563.1| interleukin-10 precursor [Homo sapiens] MHSSALLCCLVLLTGVRASPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESL LEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVE QVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN Vaximmutor IL10 Bos taurus 281246 41386772 BOS_15514 GJ062448 NP_776513 9913 16 4402473 4406411 - interleukin 10 8.14 19616.38 178 >gi|258513351:4402473-4406411 Bos taurus breed Hereford chromosome 16, Bos_taurus_UMD_3.1, whole genome shotgun sequence AAATAGCTCACAGAGAAGCTCAGTAAATAAATATATGTGGGAGCTGAGGTATCAGAGGTAATAAATACTC TAGGGGAGAGGCACAGTACAGGTCTCCAGGGAGCTGGCTCTGCTCTCCCAGAACCCTGGATTGGATTTCA GAGGTCTTCCGTTTACGTCATGGAGTCTAGTAGAGTCACCATCCTGGAGGTCTTCTTCCCTAGAATGCTT CACTTTTGCATCTTCGTTGTCATGTAGGTTTCTATGTAGTTGATGAAGATGTCAAACTCACTCATGGCTT TGTAGACACCCCTCTCTTGGAGCTGTGTGGGGAGACAAGACGTGTTCAGTGAAAACTCCCTCTGAGCAAG TGAGTGGGTCCCTGCTGGGAAGGTCATGACCCTGGGCCCAGAGGCTGCACTAGCTTGCTTGCATTGGGAG ATGAAGGACAGACTCTGGGCATCTGGAGAGATGCCGGACTGTCAGCAAAGGCTATGCGTTGCCCTGGACC TTGCCCGGTGGGCAGCAAGAGGAACATTCACTTTGCAAAGTGTGGTTTTTCGAACGGTGCTTTTCCAGCA ATTTTAAACGTCATGTGCTCACGTGACGCTTATTCAATATCTGATGAAAACACATTAGCGGAACCACTTT GAAAGAAAGACAATGAGAGAGAGAGAGAGAGGTAGCCTTGTTCCTGTTTATGTTCCCCCACCCGTGGAGT GAGCAGGCCCAGCCCCGCTGCTCTGACCTACTGCAGGGAGGTTCATGGCCCCTGATACTGGAACCTGCCC AGGGTCTGTCCCCCACCCACGAGGAGGACATAGGTTCCGCGGCTTCTGGAAAATGGGGTAGAAGAGCTCA TGGACAGCTGGAAGTGAGAAAGGCCACTTCGTTTGAGAGCTGAGATTCTCAGGTGCCTTGTGTGGTGAGG CTGGCGGGTGGAAAGAGAGGGGTCAAGTCAGGTAAGGAGAGCTCTGCTTCCTGCTCTGGGCTCGGGGTGC TCAGGCTGCGCCCCTGGGTGTGTTTGTAAGTGCACGTTCACGTGTCAGCTTCCTGCACGTGCGGCTTCAG ACTGGATCCCAACAAGCTACTTTCTGTTTCCTCTGTCACTCTCCCCAGCACCCCGCTCGCTGTGTTCACA GCTGGCGGGCCAGCCCAACCGGCAGGCCTGCGAAGGCAGGGAGCAGTCATTTAGAAACCCCCAAAGACAC TTCACAGAAAACAAATATAACTGAGGTGGCGGCCCCTCACTGGATGAAGAGGTCACCCCACTTGCCGGGT TCCCACACTCCCTCCGAGCAAAGTTCAGGGCCTGCTGAGCACCTGCCCACCTCTGCCCCTTCGAGAGTGG ATGGAGGTCACGGTCCTCAGGCCCTCCCTGCCAGCAGCCCAGCCTCACCTCACTGAAGACTCTCTTCACC TTCTCCACCGCCTTGCTCTTGTTTTCGCAGGGCAGAAAGCGATGCTGTGGAAAAGGAGAGAGTATGAGCA ACATTGGTGGCTGGTCCCAGATGCACCGTGCTTGGGAGACAGCCTGGGAGCAGGAGGAGGCCAGCTCATC TTGAAGGCCCACCCTGCATCTCAGGGAGGACTGAGCCTGGGCACCCTTCCCCACCAGGCAGAGAGGAAGC ACAGCAAGGCAGGGGCCAGGCGGCATGTGTGTCTCTAGGAGCTCCTCCCCACCGTTAACAAGGCTGCATC AACACCTCTGTCTTGGAGTTTCCTTCCGGGCGCATCCCAGTGCCAACCAGACTGCTGAGCCAGCCCTTCT TCCATCCTGTCCTGCTTCTGGCCTGTCCCCCTCCTTGGTCCCAGCCACAAGGACCATTTCTAAGTGCCCC GCAGAGCAGAGACACAGGGTCAGCCCTGCCAGCCAACCCCCTTGCCTAGCGCTGTCCTGCAAATACAGCC CAACTGATTTCTCAACATCGGCGAGGGAGCAGAGGAAACTGGTGGGGAGGGTATCATTGCAGTTGGAGGG ATCAGTAATCCTGGGGGATTATTCGCAGACACTAGCCTGGGACCAAGGATCCCCCTGGCTCTTCTAAAAT GCCAGGACAGTCCTGGCAGTCAGCCGGGCCCCACCTGAAGCTCTGCTTTCAAAAGGTCGAGAAGAAGAGG AAGGGGCCTTCCCTCTCCCAGGCCCTCTGCTTCTGCCTGCTTCCGTGGGGCCCAGGGCCTCTGTCCCCAG GGACAAGGGAGCCCTGGCTGAAGCTCTGGGCCCCTTTCACGTCGAGGCCGGGGACTGCTAATAACCCACA AATGACTCACAAATAACAGGAAAGCTATTGCAAATCTTCACATGTAAGCACTCAGCAACCACCCCCACCC GCGGCTCAGAAACAGCCGAGCCAGGCCTGGGGTTCCGGAGGGGCAGGGGCCCCGAGGGCTGGTGTCCCGG GGTCTCTGGGCGAGAGCAGAGCTTGAAGAGGAGGTCTCAGCACGAGCCCAGAGGAGCCAGAGCTGGCCTG CTACTCACACAGCGCCGCAGCCGCAGCCGGAGGGTCTTCAGCTTCTCCCCCAGTGAGTTCACGTGTTCCT TGATGTCAGGCCCGTGGTTCTCAGCCTGTGGCATCACCTCTTCCAGGTAAAACTGGATCATTTCCGACAA GGCTTGGCAACCCAGGTAACCCTGAGGGCGGAAACCAAGGCGGGTGGTGACCCGGGAGGAAGGGCTACAC TGCCTGAGAGGACGGCTCTGTTCCCCAAGGACCTTGTTCAGTGGCCACCTCCCCCTGGAACGGAGCCCTT GGCAACACCCAACACCCTGCCTCCCTCTGGCTATGTGCTGAGTTAAGATCTTCCCACTTCTCCTTTTTTT GAAGTGAAGGAAGCAGACCCAGCTCCCTGCAGGCGAAGAAGCAGAGTCTCCCTTGGGCACACCGCAAGCT CCTTGCCCCCGCCAAACGCACTCACCCCAGCCCTCACCTTAAAGTCATCCAGCAGAGACTGGGTCAACAG TAAGCTGTGCAGTTGGTCCTTCATTTGCTGCAAGAAGAACAGAAGGAGAATGAACCCAAGGTTTGGAGCA ATACTGAGAAGGACTGTCTTTCTGATATCCTTTTATTGAGAGACTTTTTGATTTTTAGTCAACACAGCCC TGTCTCCAAAAGGGAAAGGAGAGGTTTTGAAACAGGACATTCAGCAGCGCTGAAGCCCTTAGTTAAACCA GGTCCTTCCTCCGGGGATCAAGTTCTTTTAAAAACCTGGCTGATTTTGAAGGCAAGGCAGAAGCCTGCCT GGGTCCCAGTCTTCTTTCTGTAGAGCTGCGTCACGTGTGCAGGGACCCCCATTCCCAACCCCTCAGCACC CTCTGCTTTGCTGTCTCCAGCTGCAGCACTGACCGTTCTCTGGGAGTGGCAAGCATCTTCTCCTGCAGGT CTCCCCCTGTATGCGCTACCTCCAACCCATTTGTCATTCAGGTTCTGGAAGCCCTCCAAGCTGAGGCAAC TTTTGTATCCCTGCCACGGGGTTTTGGGAACATCTTCACCTCCAATCAGATCTCTCCCAGCCAATGAGGT ATTGAGCTCCAGAGGAATCCTTTACAGATTTTCTAGCATAGGCTAGATGTGCCTTTACATTTTTAAAGAC AACTTTTCTTGCACAATCTGCTACATCACCAGAGCTCAGATTAACTCACTGAACCCACAGTTTGACCAGG GACTCTTAAGAATTTCAGCTTCGAGGGGAATGGGTGCTGAGGGTGGAGGTGAGTGAAAAGGAGGCTCTTG TGAGTGCCTGCAGAATGGCTCTTCCTGCGGTCCTAAGGGAGAAATGAAGGACAGGAGGGCTTTATACTCA CAAAGAAAGTCTTCACCTTGCCGAAGGCAGCTCGGAGCTCCCGCAGCATGTGGGGCAGGCTGGTTGGCAA GTGGATACAGCTGCTGTCAGACAGGGTGCTCGCATCTCGGCTGGCTGCCACCCCAGCCAGGAAGACCAGG CAACAGAGCAGGGCTGAGC >gi|41386772|ref|NP_776513.1| interleukin-10 precursor [Bos taurus] MHSSALLCCLVFLAGVAASRDASTLSDSSCIHLPTSLPHMLRELRAAFGEAKTFFQMKDQLHSLLLTQSL LDDFKGYLGCQALSEMIQFYLEEVMPQAENHGPDIKEHVNSLGEKLKTLRLRLRRCHRFLPCENKSKAVE KVKRVFSELQERGVYKAMSEFDIFINYIETYMTTKMQK Vaximmutor Il10 (interleukin 10) Mus musculus 16153 6754318 AL513351.1 NM_010548 NM_010548 MGI:96537; Ensembl:ENSMUSG00000016529; 10090 ? >gi|6754317|ref|NM_010548.1| Mus musculus interleukin 10 (Il10), mRNA GGGGGGGGGGATTTAGAGACTTGCTCTTGCACTACCAAAGCCACAAAGCAGCCTTGCAGAAAAGAGAGCT CCATCATGCCTGGCTCAGCACTGCTATGCTGCCTGCTCTTACTGACTGGCATGAGGATCAGCAGGGGCCA GTACAGCCGGGAAGACAATAACTGCACCCACTTCCCAGTCGGCCAGAGCCACATGCTCCTAGAGCTGCGG ACTGCCTTCAGCCAGGTGAAGACTTTCTTTCAAACAAAGGACCAGCTGGACAACATACTGCTAACCGACT CCTTAATGCAGGACTTTAAGGGTTACTTGGGTTGCCAAGCCTTATCGGAAATGATCCAGTTTTACCTGGT AGAAGTGATGCCCCAGGCAGAGAAGCATGGCCCAGAAATCAAGGAGCATTTGAATTCCCTGGGTGAGAAG CTGAAGACCCTCAGGATGCGGCTGAGGCGCTGTCATCGATTTCTCCCCTGTGAAAATAAGAGCAAGGCAG TGGAGCAGGTGAAGAGTGATTTTAATAAGCTCCAAGACCAAGGTGTCTACAAGGCCATGAATGAATTTGA CATCTTCATCAACTGCATAGAAGCATACATGATGATCAAAATGAAAAGCTAAAACACCTGCAGTGTGTAT TGAGTCTGCTGGACTCCAGGACCTAGACAGAGCTCTCTAAATCTGATCCAGGGATCTTAGCTAACGGAAA CAACTCCTTGGAAAACCTCGTTTGTACCTCTCTCCGAAATATTTATTACCTCTGATACCTCAGTTCCCAT TCTATTTATTCACTGAGCTTCTCTGTGAACTATTTAGAAAGAAGCCCAATATTATAATTTTACAGTATTT ATTATTTTTAACCTGTGTTTAAGCTGTTTCCATTGGGGACACTTTATAGTATTTAAAGGGAGATTATATT ATATGATGGGAGGGGTTCTTCCTTGGGAAGCAATTGAAGCTTCTATTCTAAGGCTGGCCACACTTGAGAG CTGCAGGGCCCTTTGCTATGGTGTCCTTTCAATTGCTCTCATCCCTGAGTTCAGAGCTCCTAAGAGAGTT GTGAAGAAACTCATGGGTCTTGGGAAGAGAAACCAGGGAGATCCTTTGATGATCATTCCTGCAGCAGCTC AGAGGGTTCCCCTACTGTCATCCCCCAGCCGCTTCATCCCTGAAAACTGTGGCCAGTTTGTTATTTATAA CCACCTAAAATTAGTTCTAATAGAACTCATTTTTAACTAGAAGTAATGCAATTCCTCTGGGAATGGTGTA TTGTTTGTCTGCCTTTGTAGCAGCATCTAATTTTGAATAAATGGATCTTATTCG >gi|6754318|ref|NP_034678.1| interleukin 10 [Mus musculus] MPGSALLCCLLLLTGMRISRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSL MQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVE QVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS IL-10 plays an important role in Th2 type immune response. Il12b Mus musculus 16160 6680397 RP23-388G23.1 AL607030 NP_032378 10090 11 44400062 44414016 + interleukin 12b 6.5 35952.67 335 Also known as p40; Il-12b; Il12p40; Il-12p40 >gi|372099099:44400062-44414016 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J CAGAAGGAACAGTGGGTGTCCAGGCACATCAGACCAGGCAGCTCGCAGCAAAGCAAGGTAAGTTCTCTCC TCTTCCCTGTCGCTAACTCCCTGCATCTAGAGGCTGTCCAGATTCAGACTCCAGGGGACAGGCTACCCCT GAACCAGGCAGCGTGGGAGTGGGGTAAGTGGATTCTGGGAGCATCTCGGATGGCTTTCCCCGCTGGTGGA AACCAGGGGCTTTGACGACAGGGGCTTCAAATAGTCATTTAAAAAGTCAGCTAATTACAGATGTGATGGC GCACACCTGTAATCTCCAAATCTGGGAGGCAGGAAACAGAATGCACAGGAGTTTAAGGCCAGATTCAGCT GTGTAATGAGTTTGAGAGAGTCTCAAAAAATTGAGCTACTAATAATATGTGCCAAGCATTGTGGACAGAT GTGTATTTGATAGAAAGGTTTGCTTTGTCTATATAGACACATATACTATGTGCATTTTCTTAAGTATTAA TGCATATGTGTTAAGGAGTGTTGTTAAAATTCTGTTCTTGCCTTAAGAGTGAAACTTAAAAAAAAAAAAA GCCTTTCATGTACCCCTTCAAGCCTAAGCTCATCACTGAATGTCCTACCTGTGATGGCCTTTAGAATCAG CTATGATAATCAAAGTGGAGAGCTGGGTTTGGGGGTTCCTTTGGATTTTAACTAACTTTGCCACCTCTTC AAAACTGGAAAGCCCAAGGCATGTTCCAAACTATTTCCTAAAGGGTTCTAAAATCTGTGAGAAAGGGAAG GCTAATCTTCATGGGTTGGCTTCCTCTGCTTGCCCAGGTGAAATCATGAGCACCAGATGGGGCAGGGTGA TGGGGCAGGGTGACATCTTGCTTTCACCCAGGGATCACTGAGCCTCAGCTGGGGGCTCCGTTTGTTCAAG CAAGGCATTTCAAGATGAAGTTAGGTCACCCCCAAGAATTCATTTTGCTGAGATCTGAAATACTGTGATT ATTGCACCAACAATCCATGGACTCTCTCTAGGCTAGATTCTGGATATAAGACTAGACAGATATGACCTCT GTCACACACCTCGGTATGGGAAAGGGGGGACAGACATTAAAGACGTAATCAAATGATTGTCATTTCAGCG ATGCTGAAGGGGAAGAGTAGCTATTTGGGGTCCTGTTTTCATTTGGGGCTCAAGCTGGCACTTTCTGTGG ATCATTTCATGAACTCCTAACCTGACAGAGTTATTTTTAATCTAGGAGTACAGGCTTGACAAAGTCAACA CAACTTGTCAAGGTCACATAACTAACAAGTGGCAGACTGAGGTCTGTTTGGCTGTAGAACCAAGGCCCTT TGCCCGTGCCCTCCTTGCCATCCCTGACAGCCACGCCATCTGTACTGTGCATTGTTGTTTCTGGGCATTT TTATTTTTACATATCATTTCTCTTTTAAATTAAAGAATAACAGAATGAAACTTGCAAAAGGGATGTCCAC TCAGGCACCCGCTGGCAGCAATGTTTGGAAGCATTTGCATGCTCTAGGGAGCTAGAGTATCCAGCCCAGG GTTCCTGTGCTCTTTGCACAAGACTTTCAGGACTTAAGAAAACTAATGAAAACAAACAAACAAACAAACA AACAAAAAAGAATTGTTAAAACATAGCATATGGAAGAATTGTGGAGTGAGAGGGAAACAAAGCCCAGCAT CTGCCAATCAGCCTGAAAGCCAAAGCCTGGCTACATACTGCACACTTTTTTGCTTGTGCAGATTTGATTA AGAGGCGACCTGCCATCATCTCCACCCTCTGCTGGTCTTCTATGATCTAAGATGTAGGTGATGCCGAAAT GAAGATTGTTCCTTGGACCATTGCCAGGGCTCCCTTCAGCCTGAAATATGGTTCACGTGTGATGCTCAAG TGCATCTGAGGCTGACAGAGGAAATGCTTGGCATGTAGCCAAAGGGGAAATCCCTGTGTTGAGAGCATAT GAAGAGATCACCAGGAGATGCCAGGAATCTCTGCCTTTTTTCCCTGCCCAGAGTCCTACCATCTGGGTTT CACAGCCGTGTATAATTGCATCACCTTCTCTGGGCATTTCTCTCTACAGCCTGGCAGATTTTTATTTTTC AACCCAGTGTGCACAAAACAGAAAGAATTTCAATTTTTTTCCTTAGCTTTTTACTAGTGCAAGGTATTAT ACAATGTCATCTCACTGCATGCCCAATAGCTCTTGAAGATCACATAAGCCTTATGTCTTTTTATATGAAG GCAGTCGGGCTCAGAGGAACTAAACCAACACCAAACAGCTACTGAGTAGTAGGGCCAGAATCCAAGTCCA TGTCTGCTTGACTCCAAGCCTATGAATGTAGCAACCAGCACCATTTCAGCTTGTACTTGTCTTGACTATA TGATAATTTGGACCTAGACATGTCCTGACTCCTAGTCCTGTCTCTGTCATGAAAAAGAAATCATAGCAGG GCCCTCAAGAGGTTAGCAGTACTTCTGTTCTCTCAAAGGTCGTGATTTCTGACCAAGGCCTGTTTATTTT TAAGGCATTTGCCTATAACATTTAAGCCTTAATTCATATGGGTTAGACTGTGGTCTCTCAAATCTTGTTC CTGACGGGCGGTGGCACCCTCCAGGATAGATGAAAAGTATTTCTGAAGGGAATTAGTAATCTACACTGAT TATCTCAACCACATTTATGAGTGAGGTATAATCTTTACAGTCTAAAGGTTTGTGTTGCCCTGGTGTTAAT CTAGACTGGGTTACAATACAGGATGGTTACTGACTTATCTATTTCATGATAGGTTAATCAATATGGTACA GTCTTCTGCCAAAGAACACCTTGAATTTTAGGAGTTCTGGACTCTCAAACCTAGCTAGCACATTTGCCCT CACAATGTGTTCAATGGATCATAACATCAGCTTTCAGCACCTCCAAGACATGTTAAGTCAGACACTCTGT TCTGGGCTGAGACCCAACAGTCTCCGTGAACCCAGGTTATACACACACCCAAGCTTAAGAAATGCTGCAT TTTGTTGTGAGGGAGAGAATTATTTTAGAATTTGGAGTAATCTCTGCCTAGGTTTAAAAAAACCTAATCC ACTACAGGTCCTTGTCAGAACTTGAAAGTTTACCCTGTGTCACCTTTCCCACACCTAAGCTGGTCCTCTA GCCTGGGGCATCCCAGTGCCTACTGCACAGCAGGGTGATTCGGATGCCCAGTGACACTGTTCTCGTCCAT ACTGGCAGCATGGCAGTACTTTCCCATTCTGTTCAAAGCTCTTTGGGTATTATGTTAATATTACAATCAT AACTAAGGAAATATCTGATGACTGTGGACCTGGGAAGCCACGGTCATCTCTAAGTTTATGTTTCTGAACC TTAAAAAAAAATAAATAAGATGGGTGTGGTGGCTCTCGCCTTTAATCCCAGCACTCAGGAAGCAGAGGCA GGTGGATCCCTGTGAGTTTGGGGTCAGCCTTATCTACATAGAGTTCCAGGACAACGAAGGCTGAAAGTCT GTCTTAAGAAGAGGGAGGGAGGAGATGATTGTGGATATCAGGAAGACAGTTCAGCAGGTCACATTACTTC TGCACAAGCCTGGTGACCTGGGTTTGAACCCTGAACCCAGAGTGAAAGGAGACAGTCAACTCCCAAAAGT TGTCCTCTGACCTCCACACATGCACACATACAATAGTCATACATCAATATATTTTTAAAAAACAAAATGG TATCAGGTAGCTGGGCCCTATGATTATTTAAGCTCCAGGTAGCCTCTAGTTCTTTATTTGGAGATGTTTT TACCCAAATCTAGAAGAGACCTCCTATAATCCTGGTCCAACAGTGTGAAAAGAAAGGGGAAAGTGAGTAT TTGGAAGGCAGATAAGCTGTTGATCGGGTTAATTGGATAACAGTTGACATTCTCTCTCCCATTTCCCCGC CTTTTCCTTCTCCTCTGCTCCCCTTCTTCCTCCCTCCTGCCCCTCCTCCACACAGATGTGTCCTCAGAAG CTAACCATCTCCTGGTTTGCCATCGTTTTGCTGGTGTCTCCACTCATGGCCATGTGGGAGCTGGAGAAAG ACGGTAAGCAGCCTTTTCCCTGTTTTCTTTGGAGCGCCAGCACCTAAACTTGATCTCCAGGCAGCCTGCT GGGAAGGGATAGTGAAGTCTGAAGTAGGCAACCGTGCATGGGATTGTCTCAGGAAAAATCCTCAGGGAAG ATGGAGGGTTAGCGTCTCTTCCTCCACCTATCTCTCTCCCTTGTTTGGTTTTCATGTTGGATGACAGGAA AGGAGTTTCAGGTGAACTATGGTGGTGTGTGGGATATGATCTTGGCCTTAGATACCAGAAAATGACTCTT CTAAACAATTGAAATTCCCTAATAGAGAATTTGTAACGTAGCAAGATTGTCTTCTACCCTCTCTTGTCTT GCATATTACAACGTGCTCTCAGTGGGCACCATGATGGTTGTCCTCTGGCTATTTTGGGATTGGCATTCAC ACTGACTTGGTTTCAGGTCATGGCTCTGCCAGGTATTGATACTGAACCTTTGAGGCTGGGTAGTCTTGTG ACCTCAGTTTTCTTTCCTTTTTTTTTCCCCCAGACAGGGTTTCTCTATATAGCCTTGACTTGTCCTGAAA CTCACTCTGTAGACCAGGCTGGCCTTAAACTCAGAAATCTGCCTGCCTCTGCCTCCCGAGTGCTGAGATT AAAGGAATGCACCACCACTGCCTGGCTGACCTCAGTTTTCTTAATTGGGTGTTCAGTATAGCACAGCCCA CATGGACTATTGAATATGTTAATAAATACAGAAAATAACATATGGTAGAGTGAGCAAGCACTATTATTTA AAACAAAAAGTCTCTGAACTTTTCATTATCCCAATGGCAATAAGATTATTAGTAGACAAATAATGAAGGC ATAGAAGGGGGTGCTCTCTGTCCTCTGTCCTTGTGCCATGTCACAGTTAGCAAATGTCCATCAACTCAGT CCTCAGATGGTCGTCACAGGATCTCAAATGTTTCATGGAATACAAGGTAATCAAGTCAGGAATTTCTGCT TTTATAGCTACACTTTAGTAATAATTAATATTTATTTAACACTCAATTTCGCAACAACCATGTAAAGTAG GAGCTATCTGTATTCCATTTTACAGATAGTAAAAACTGAGGTTCAAGAGGCTAAGTAAGTTTCCCACAAG GTCCAGAATAGAACTTGATCCCAGTTTAGTCTCACTTCAGAGCCTGTGTGCTTAAATGCTAGATTTTACA AGTAAGGCGTGAAGCAACTATTTCCTGTGGCCATAAAGAGGAAATGGGTGTAAATTATAAATGAAAAGAT TTCCCAGCTGCTGATTAGAAATAATCTTCTGAGAATCCAGGAAAAAAAAAAAAGGTGAGCCTTTTTTTCA TTCTGAAGAATTACAAGAGTTTGAACGATATTCGCAGGTAAAGGTGCTTGCCACCAAGTCCGATGACCTA GACTTCATCACTGCGATCAGCATGATGGAAGGAGAGGATCGAGTTCTCTAAATTGCCCTCTGCTCTCAAG ACATGAGCTGTGACACCTGTGCACACACACACACACACACACACACATGAAAGAATTAAATGCAACTTTA AAAAGAGTAATCTCCAGCCTGGCCACCCACGCCAACAGAATTTTGGATGCTTTCCTAAGTAAAGCCTTTG CCACAGCCAGATGGACAACTTAGGCCCTGGCAGAGTTAACCCCTTAGTTACTTCTGACCTGATAAACCAC AGAAATTTCAGAAAAGCAACAGGAGAAACACAGAGAGAGCTTGTGTTGGAGTCACAATGGTGGCCCGAAG CCCAGCAGATATCTAAGCTCTGATTCCAACGTGTCCAAGAAGTTCTTTCCCTGCCCTGGGGCTAAGGTCT TCTAGGGTTTCTGAGTTCAGGTTCTAAAAGGAAAATAATAGACAGCCTGTTTCATGGCCTAGGGGACAAA GGGAAACTGGGAGCCAAAGGGTCCTGTGCACTGGAATCAGCTCAGGCTAACGATTTCTGAAGAGCTTGGC CTCAAGTGATTTGTACCTCTTATCTTTGCTCCCCATTCAGCAGTGACAAATCCCACTGATGTAAAAGTAA GAGCTTAGCATTTCACAAGGCTTTAAGCTCCAATGCCAATCACAAAGCCACCATTCACAGGGCACTTAAG GAAACTGTCTTAAGCTTGCATACAAATTAGTTTAGATGTTACCTTCTAAAGAAAGCACTAATATTTTGCT TTCTTTTTTCTTTTTTTAAAAGAATGACATCAGACTAGGGCATGGCAGTTCATGCCTGCAATCCCTGAAC TTGATAGATCAAGACAAGAAGAAAGCCCAATTTTGTGGCCAGCCGGGGCTATAAAGTAAAATTCTAAGTA GCTGGCTGTCTCTTCCCACCCCCGAGGCCATGGCTCTCTGAAAGCCCTTTGCTGGCTCTGCTCCATAGCC GTGCTATTCCAAAACCCAGGCTCTTCACCACTATGCCTTCCATATTCAATCAGGCATCAGATGACTGGGG CCATCCTGATCTGACTCAGCAACCAAGAACAAACCTCACTGATGGGTTTTCAGGTGATGCTGATGATGGA GGTTGAAAGACGACCCTGCTTTACGGGTCACTGCATTAAGCTAGTCTTTCGATTAATAATTGATATGTAG GTAACCTTTGAAGCTTTGTCACATGAGGCTTTGGTGTGACCCACTCCAGAATACCTTATGGAGATATTCT GGAGGCTCCTTCAGAAACTAGCATCAGATATCCATTGCACAACACAGGAGACAAAAGATCCGAAAGACAC CATTTGTTACCCTTCACACTTGATGGGACCTCACTTAGCCCTTGGCCATCAGCAAAAACAGTTCCTTCAA AAACTGAGACAATCATCTACTGCTTTGTAAGAGTGTAAAATAGCTTTTAAAAACAAGTAGTTAATGGCAA TCTCCGTCCACAAATTTCATGCAGAAGAGACTTAAAAAAAAAAAAACCTAAATCTTTCTGGATAATTTTA AGAGTTTTTCAGTGCATTTGCCTGGCTCTTTGGCCTGTGCATGACAGTGATGATGATGATGGTGGTGGTG GTGGTGATGATGATGATTTTAAAAAGCTAGCACAGGGTTATCCTGCCGTAACCTTAGCTGTGCTAAGGTG CATGCTTTTTTACCATCTATCCTCACAGCTAATCCTATTAGCATAGACAATATTGTTCTTACCATTTTGT GAGGACTCTGGGGCTACTGGTGGTTCAGGACCTTGCTCAAAATCTCCTTTGCAATTAGAGAAGATAGGAT TTCTGCATCTTTCTTTCTCCACACCTATGCTTTAAGCCACTATGTTATTCTGCTCAATACTCTGCCCTGT GTATTCCCTGTGAATTACAACTGGAAGCTTACTCCCCCTCATCTCCATTCCCAACACACACACACACACA CACACACACACACACACACACACACACACTAGTGGCTTCTGACAAGGAATCTTGTGCTGTTTGCATCTTT TCTTGTTTCAAGGTCTTCCCTTCCTAATCAGAGACCGATTATTACTGGCCACCTGCCAGTTAAATGACTC GGTGGCAGCTATAGAACTGTTTCTCTGTTCTGTCCCCATGCCCTGAGATCTCTCAGCAAGCACTTGCCAA ACTCCTGTGAGCTATGACGTTCCAAGGGCCATGGGTGTGTCAAGACACTGAGTGAAACCCAGTTCCCGGG GTTTGTGAGGCTCCTGGTCCAGGTCCTCACTGCTGGTGCTCTCCAGTTTATGTTGTAGAGGTGGACTGGA CTCCCGATGCCCCTGGAGAAACAGTGAACCTCACCTGTGACACGCCTGAAGAAGATGACATCACCTGGAC CTCAGACCAGAGACATGGAGTCATAGGCTCTGGAAAGACCCTGACCATCACTGTCAAAGAGTTTCTAGAT GCTGGCCAGTACACCTGCCACAAAGGAGGCGAGACTCTGAGCCACTCACATCTGCTGCTCCACAAGAAGG AAAATGGAATTTGGTCCACTGAAATTTTAAAAAGTAATTCCATCCCCCTTGACAGTATCTTTTCTTCTAC CCCTCCTTAAAAAGTAATAATTGCAAGTGTGTGTGTGTGTGTGTGTGTGTGCGCGTGCGCGTGCGCGTGC ACTGAGGCTCAGAAATGTTTCTCATTTCCTTTGAAACAGGAACTCTCATTGGCCCATAGCTTACCAATAA GCTTTCTTGGTTAGCCAATAACATCCAGAGACCTTGTCTCAAAAAAAAAAAAAAAAAAATCTCACCCTTG TACCATGTAATGGCCAGTGATCCATCCACAGTAAAAAAGAAATAAATAAATAAAATAAAAATAAAAAATA ACAAAAACAAATTTCTTTATTAAAAGAAAACTCCTAAATATTTAATTTTTCTTTTCTTTTCAAATGCAGA TTTCAAAAACAAGACTTTCCTGAAGTGTGAAGCACCAAATTACTCCGGACGGTTCACGTGCTCATGGCTG GTGCAAAGAAACATGGACTTGAAGTTCAACATCAAGAGCAGTAGCAGGTAAGGGAGCTGCCATGATTTCT CAAGGTTTTGTGTGATCTACTTCATAACAAAAAGAAGTACAAACTGCCCATGGATTATCAGTGACTGGTA ATGGAACAGCAAAGTCAATTCTGTATTTTTTTTTTCAACTTCCCTCTGCTCGCCTAAGGAAATAGCATGG AGCTTTAAGCCAGCCCTCTGAGACTAGTTGGCTGGGTAGTTTGAATTCACTCATCCGAATTGCCTGGCCG TGCTTCATTAAGGCTCACAGCACCATGGGGAGGAATTAAAAAGAAGCCACAGCTACAAATAACTGGCCAG AGCTCGACCTTGTGTCAAAAGCAGAATTGAGTGCTCCAGGGCCTTGCTGTTGGAAGCCCTGGAACAGATT CCAGAACCCTCTGAGGCCTTTCGAGATTTTCCTAGAGCCTTTTCAACTTCTTCCCCCCCCCACCCCCACC CCCCAACCGTTATTCTGAAGTTCAGTTTCCCCAATGCACTTGGGCTTTTCCTAAAAACATCATCTCCCAG GGCTGCAGAGCGGGTGTGTCAGACTGCTCTACAGCTGACTTTAGTCAGCAACTAAATCCCTGAGCCACAT GGAGGGCAGGGAGTGAGCGCCCTGGCTCTCCCACTATTTAAATGAATAAACGGGGGACAGGGGGTGTGGG GGGGACGACTCCCTTTTCCATCTTTATCATGAATTTGCTATGACTTAACTTAGCTAAGGAAAATAATGGT TCAGGGTGACATCAGAGTCCTTGTTGCTTCTATGAGAATTTTCTGATCTGGTAAAGGTGGCCCCAAAGGA GACCAGTGTACAATGCAGATGCTTACATATAGAAACATGCCAGACCTCTATGTGCTTTAAAAAATTAGAC AAGCACGATACATTTTCCTGCTAGAGAAATCTTGCACGTGTTCCTGAAGAAACTAGTACATGGATAAGGG TCAGTGGAACATTTTGCATAATTAGCAAAAGCATGCTCAAATGGAACATCAATGTGCATCTATTGAAATA AATAACTCAACTTTCGTGTTCTGTTTAGAGAACTCCCCCACAATACTTTCAGAGAGGAAAAAGACGTTAC AAACTCATGCATATATATTTTTCATTTGTCTTTAAAGTTGTGCTACTCTTTGGATAAACTACATATGTAC ATATATGTCTGTGTGCATGTCTATCTTAAATATTCATTTGGAAAAACTCTAGAAGTAACAAACTGTGAAC AATGTCTATATGTGGGACTCAGATTTGAGTAAAAAGGAGAATTGTGGCGACACGCATGTTTTCTTTATAA TCATCTTTGTTGTTATAATTCTTTATAATTCAGCAGTTTCTCTTGCATTACGATATTTTCAGGTTCATTT AGGCAGAGATGCAACTTTCAGTCTCTTACAGCTAAGCTTCCACTCTGCAAAGGGCAAGGGGGTTTTTGTT TGGTTTTGGTTTTGGTTGTTTTATTCCACTGGAGAAATCCAACCAGCTGGACAGACTGGCCTCGGACTGT AGACACCAGGCACTCTATCTCTGATCTATAATACAGAACTCATTCTGTCTTTGCCATCAGTTCCCCTGAC TCTCGGGCAGTGACATGTGGAATGGCGTCTCTGTCTGCAGAGAAGGTCACACTGGACCAAAGGGACTATG AGAAGTATTCAGTGTCCTGCCAGGAGGATGTCACCTGCCCAACTGCCGAGGAGACCCTGCCCATTGAACT GGCGTTGGAAGCACGGCAGCAGAATAAATATGAGAACTACAGCACCAGCTTCTTCATCAGGGACATCAGT GAGTTTTGCCTGGGAGCAGGTGCTAGGGAAGCAAAGACGATGTCTGCCACGTGTCCATGATACCCTGGGG CATAGGATTGGCCTGCATTCTTTTCCATTCTCCTAAAAAGCATGATACAGACACTACCCTCTTCATCTTA CAGATGAGGAAATTAAAGGGTTAAGCTAACCTTTTTCCAGATCTCAAATCTCACAGACAGCAGACCAGAC CCAGACCTGCCCCAATGGTGGCACCTAATGGATTATGGGTTAACTACATGCTTAGTGTAAAGCAGAAACC AAGCACAGGGAGGGTTCCCTGATTGCTGAGCCCAGATACGAGACTCTATTAAGCAGAATGACTCAACCAG TAGGGACTTCCGGTCTGTGCAGTGCAGAAAGGGTTCTAAAATGTAGGATATGGCCAATAATGGTGACTTC CTCGAGTGGAAAGATCACTCTCAGCAGATCCCTCACAGGTGCTGCCCTGATGCTCAGAGAAGTGAAAGAG CTATTCCAGAGACTAGAAGGTGGAGGGCTCTGTCAGCCAAGCTCAGGAGCCTAAGTCCTAGCTCCAAAGC CTGGCCAGGATAATAAAGGATGCTTGTCTTCTTCTGCAGTCAAACCAGACCCGCCCAAGAACTTGCAGAT GAAGCCTTTGAAGAACTCACAGGTGGAGGTCAGCTGGGAGTACCCTGACTCCTGGAGCACTCCCCATTCC TACTTCTCCCTCAAGTTCTTTGTTCGAATCCAGCGCAAGAAAGAAAAGATGAAGGAGACAGAGGAGGGGT GTAACCAGGTAGGATTTATGCAGCCCATGTTGGGGCCATTGGAAAGATCCTGACAACCAGGTGCTTGCCT CAGGACCAGAACTGTGTTAAGGGCTCTCCATGTGAATCACACACAACCTATACAAAGTCCCAAAGGCAGC CAGTATTGCTGTCACCTCTCCACAGAGGCCAGGAAAGCTCAGAGAGGCTATGTTTTGCCCACGTTCTTCC AGCTAGAGCAGTGGCAGAACCAGTACCCAGCAATGGTTTGATGGAGAAAGAGATGGTTAGCTCAACCACT TCAATTCCTCCAGTATAGTCGATTGTCAATCCTCAACCAAAGTTGTTCATGATATAGAAACCTGGGATCC AGGAGCCTATAATGATTGTTTAAACTGAGTAAATATAGTAAATGCTAAAACATTGATGCTGGGACCCACC AAATAAGGATAATCTCTACTTCCTTAATATGAGCAATCATTTCACCTTACAAGATGTCCACCTTTACTGG GCTAATATGGTGACTTTCTACCTTTCGGGTCCTTAGCACAGTGATCTCTTGCTAGGTTTGAGGAACACTT TTATGAGCATTTAAGTCACAGAGCAAAGGATTCTTCCAACCTAGCATTGAGAATGTGATAGCAATTCAGC TCAGAGATTCAACCACTTCCTAAAAGCAGGAGCAAAAAATGGGATTATTTTGAAACAGATTTTAACTGCT ACCTAAGTTTCATCCCTGTGTGTATGTATTTAATATACCAATCTTAAATAGGTAATGTCTAGTGCTTTCT ACTTGCTAAAAGCTTTCTACGCATTTTTACATAAATGTTGTATTTAGCTGCTGCAATTCTTAGAGGCAGG CATCCTGGGACCTACTCATGAATAAGGAGAGACACAGAGAACCAGAGATATTTTCCTGAAGCACCACAGC TGTTTAGTAGCATTGCCAGGATTTGAAGTCACTTTTGTAAGCTGACAGGCATGGTAATTCTTGATGTCAA GACAGCTAAAGAAAGCTGGTCTCAGTCCCAACATCAGCTTCATATGGGAGTGGTTACCCATGGGACCTTG GGGTAATGCTGCTACTTCAAGAGGCAGAAGGGGGAGCAAGCCATTTCTGTTGGAGTTTACTAAATAGAAA AGTGAGAGTGGGGTGGTGGATGAATCATGCTGGAATATGAAGACCTGAATTTTAACCCCCAGAACCTCTG TAAAACTGAGTAGTAATGCCTGAACCTGGGCTCTGGAAAGAGGTGGGACACATACACACACACACACACA CACACACACACACACACACACACACACACACACCTCTGATTATAGCTTCTACCAGCAGGTGGTGCAAGGG GAGCGCCCATGCCAGCCTGCCTTATATTGCCTAAAGCCCTTACTTAAATTTTCCTCTCCTTTGCAGAAAG GTGCGTTCCTCGTAGAGAAGACATCTACCGAAGTCCAATGCAAAGGCGGGAATGTCTGCGTGCAAGCTCA GGATCGCTATTACAATTCCTCATGCAGCAAGTGGGCATGTGTTCCCTGCAGGTGTGTGAGCAGGACCGTG AAGCCCAACCACACCTGCACATTTAGTGGTCCTGGTGGAGGACAACAGGAGGCTTGTGAGGGAGGTGGGG GTGGAATTCCTGGCATAGGGATTCCAGTCAGAAACACATTTGTTCATCAGCAGATGTTAACTTGATGTGT ATGTTAGGTCAGGCACTATGCTAGGCCAAGGATATTACAATAAAGAAAACAAGCATGGGTCCTACCCTTG TACAGGGAAAGGCAAGAAGGACCAGAGGAGGAGGAAATCAGGAGTCAGAGTATGGAGAACTTGGAAGTAG ACAGAAGTTGGAACCATATCTTAGGGGTTCTGGGGAACCACTGAAGTCTTTTTAATAAGGAAATATCCCA ATCAAAGCCCTTTTAAGAAAAATCTCTCAAGAGAGTCCCTGGCAAGCATGAGACCAGCTGAAGTCCAAGC CCTGTAAGGAAGGGTTGGGCTGTCTCGCACAGAAATCCTCAAGACCCAGTGTCTGGGGATAATAATGTGT GGTGTGAAATAGGAGGGTATTGAGGCTATACCTCTGACCAATATGGACTTACCTACCCACATTCCTTTAC AGGGTCCGATCCTAGGATGCAACGTTGGAAAGGAAAGAAAAGTGGAAGACATTAAGGAAGAAAAATTTAA ACTCAGGATGGAAGAGTCCCCCAAAAGCTGTCTTCTGCTTGGTTGGCTTTTTCCAGTTTTCCTAAGTTCA TCATGACACCTTTGCTGATTTCTACATGTAAATGTTAAATGCCCGCAGAGCCAGGGAGCTAATGTATGCA TAGATATTCTAGCATTCCACTTGGCCTTATGCTGTTGAAATATTTAAGTAATTTATGTATTTATTAATTT ATTTCTGCATTTCACATTTGTATACCAAGATGTATTGAATATTTCATGTGCTCGTGGCCTGATCCACTGG GACCAGGCCCTATTATGCAAATTGTGAGCTTGTTATCTTCTTCAACAGCTCTTCAATCAGGGCTGCGTAG GTACATTAGCTTTTGTGACAACCAATAAGAACATAATATTCTGACACAAGCAGTGTTACATATTTGTGAC CAGTAAAGACATAGGTGGTATTTGGAGACATGAAGAAGCTGTAAAGTTGACTCTGAAGAGTTTAGCACTA GTTTCAACACCAAGAAAGACTTTTTAGAAGTGATATTGATAAGAAACCAGGGCCTTCTTTAGAAGGGTAC CTAAATTTAAAAGAATTTTGAAAGGCTGGGTATCGGTGGTATATGCTTTTAATTCCAGCACTCAGGAGAC CAAGGCAGGCAGATCTCTGTGAGTTTGAGGACAGCCTGGTGTACAGAGGGAGTTCCAGCACAGCCAGTGC CACACAGAAATTCTGTCTCAAAAAC >gi|6680397|ref|NP_032378.1| interleukin-12 subunit beta precursor [Mus musculus] MCPQKLTISWFAIVLLVSPLMAMWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIG SGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCS WLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELAL EARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEK MKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS Vaximmutor Il15 Mus musculus 16168 363000984 AC156993 NP_001241676 10090 8 82331623 82402585 - transcript variant 2 4.96 17600.72 162 Also known as IL-15; AI503618 >gi|372099102:82331623-82402585 Mus musculus strain C57BL/6J chromosome 8, GRCm38 C57BL/6J GTTAAATTTCAATTGATAAAATATCCATACAACTTTATTCCACATCTTCTGTTATATTACAAATATGTAC AAATGAAGCAATAAATTAAAAATAAGCAAGCAAAAATAAGTCTGAGACGAGCTCTTTTCCTCTCTTCATT TCCAAGATTTCATGTGATCCAAGTGGCTCATTATCTCCTTGTTAGGAAGCTTAGTTTGCCCAGCAGATTT CAAATGCATGGAGCTGTGCTGCCTCTGAGCAGCAGGTGGAGGTACCTTAATAACAGAAACACGGAAGAGG CTCGCATGCAGTCAGGACGTGTTGATGAACATTTGGACAATGCGTATAAAGCTTTGCAAAAACTCTGTGA AGGTTTTCTCCTCCAGCTCCTCACATTCCTTGCAGCCAGATTCTGCTACATTCTGCCAAATAAGGAGGGA GAATTAGATCTGCAGTCAGAACACACTTCTTCGTTGGAGGATATCATACTATTGCAACACATGGTGGAGT CTTCAGACATGAAAACATTGGCAAATGAAATCAAGTTTTGAAAAATAGTGAGAAAGAGTCAAGAGATTTA TAGGGGTTGGGTATTCGTACGTTGGATTAAAATCTTTGCAAATGTACAGGACATTCATGTTACATTGTGT CCTTCCTGCTTCAAGCCCATCACTGTGTTGTTGTTGCTGTTGTTGTTGTTGTGGTGGTGGTTTTGATTAT TTTTCCTTGTTGTGGTATTTCTTTTTCTTATCTTTCGTATCTCTCTCAAAGTCTGTACTTCATGAGCACT TGTCTTTCTGTTCTATACATATAGTGTGTATGTGTGTGTGATATATATCACATATATAATAGTTAGATAT GCATATATCACAGACTTTTGGCCTTTTTACACCACTTTTAGCCTTTTACACTACTGTTGCCTAACTACTA TTCTTGGGAAGCATAGCCATCTATCTTTTGTTTCCCAGCAATAAACACAAGGCTTGGCACAGAGTAGCTA ACTGAATATTATTGGTTGATCTGAACCACTTAGGCAAATGCCATAAACATAGCTTTGGGGACAGCTAGAA TACTATAAGTGTAGCCACCATGAATAAACATTTTAGAGATCATCTGAACCACGGAGGCTGTGCCAGCAGA AATCAAAATTATATTTCATGTTCATTAGGCACTTGGAATTCAGATTCCCCAATATGTATAAGGCACACAA TGAATGCCAGACACTGCTGTAATGCTAATAGGATTCTCTAGTCGGTCCAGAAACAATTTGAAAGAGGAAA GCAAACATGCTTAGTGGTTCATCGCCACTGACATGTAAACTGAAATTCTACACAAGTCTCCTAACCATGC AGTCCTGTTCATTCTTCCAGTATTTATTTAGGCCAAACTGTCACTTTGGGGGCTCCAACATGCAGCCTAA AGCTCAGCATTGATACTGTGATGAGTTATCTGTTACCATTAATAGGCAATGATTCACTTGGTAAGAATCC CAAGTAACGGATACCTGTAAGTTTATGTACTGGGAATTGTAATAACACAGTCAAAAGAACTCTAAAATGT ATGTACTCTGATGCTGACTTTCTAATGGTGACAGATGATTTCAGCTACTGACTCTAGGGCATAATTATCA GTGTGGGGCATATATATATATATATATATATATATATATATATAGGTGGGAACAGTGAGCAAGGCTGTAG GGAAAGCATGGCAAATATGAATAAGTGATATCACTGAGGATGTAGCATCTGAGCAGACACTGAAGCAGCC CAGCATTTGGGAAGAGAAGCCAAGACATCTCAAGATCACTGTACCTCCAGTTGTGTCTGCCTAAAGACGC TCCCGGAGTGTCCCTGACATCCATTCCCTGACTTCTGTGTAGCATACCACCTTATTGACTAACTGCTCTT CACAAACACACACACAGACACACACAGACACACATAGACACACACACACACACACACACACACACACATA TTACCTCCTTCCTCAGAACATGTGCTTCTTAAAGGGATATGTTGAAGTGCATACTAAACGAAACAATTTT ACTATTCCCCCTCTGATGATAATAATCCTTTGTTTGAATCTTGTTTTCTCACTATGGTTGTCCCTCTCAG GCCACAATTCCTTGTACCTCAGGACATTTGTGTCACTTACCCTTTAGCCAACCACAGGAGTCACTAGTAT TTGTTTCTTGTAACCCACTCCTGCTCATGTTGTCTGGGATGTTCTAAATAATACTTGAATTGTTAACACT TTTCATCATTCTAATTTTTCCATTACTTAAACGTTTAGAAGGTGAGAGGGCAGAAGCTGGAGTAGAATCA CAGCTCTAATGCCTTTGGCTGCACAAACTGGGGCATGTGGTTAGCTTTCTGCTTCCCATGTGCATCAACA TTCGAACAAGAACAGAAATAGCCAGCCATGAAAGAAGGGAAGTGACCAGTCTCTGAGACTGATTTTGAGG ATTTAGTACCTTATCAGTCTAAAGTATAAAGATCAATGTGTGCTATTAAGACCTAACCCAATAAATGTGA GCTACTGTTTCTGATGCTAAACACTTGATCACATTAATGTCTATCCACTGCACCCACTTGCCAATAAGAC ATTTTATTTTAAATCAATATGACTCTGACAGGGAGCACACTGGTTGGCATGATTCAGAAATGTCTGTTTA CCAAGGAGACCAACATGTTTAGGATTCTGTGCATGCCAGCACTGGTACCTCTTTTGGACACCACTCCACT GGAATCAAATTCTCTTCTGTGTGAACCACAGGAGACTCTCCTTATATATACATGCCTTATTTCAAGCAAG CCAAAACTAGTCAGATGCAGCTCTCTCTGGATGCTGGACTACTCACCTTGTTAGAAGACAGAGTGCTGTT TGCAAGGTAGAGCACGTTTCTTACTGTTTCATTAAGAGTCATGTTACTGTACTCATGTAAAATAACCTGC AATTCCAGGAGAAAGCAGTTCATTGCAGTAACTTTGCAACTGGGCTAAAAAGAAAAGAGCCAATTTAAAA AAAATAAACAAAAAACCTTTTCCCATTATTCATTTTCATATTGATAAGACTTTGATTGAAAAAAAAAAGT CAAATCCATCTAGTTATTTTACAACGAAACGTAATATCCACACTGCTTAGTTTTAATTGTCAACTTGACA CAACTAAGAACATAGTCTTAATGGGGACCACTTCTCAGATAACCTGTAGGCATGTCTGTAGAAGCTTGTC TTCCTTGTTAATTCATGGAGGAAGGGCCAAACTAGTGTGGTGGCACCATTCCCTAGGCAGAGGGTCCTGA ACAGTATAAGACAGAGGAGAGCCACCTGAGAGCGAGCAAGCAAGCAAGCATTCTCGCAGTTACTGTGTCT CTGTTCTTCACTGTTAGCTGGATACTTTAAGTTCCAGTCCTGACTTCCCATAACTATGGACTACAACTTG GAACTGTAAGCCAAATAAACACTTTCTTCCCTAAATCACTTTCCGACTGTGTGCCAGCAGGGAGTTTTAT TTACACAGGAAGTGCCTTTCTTTTTCCATTTGATTTTGAAATAAAAACCAAAACCACTAATAGAGAAGGA TAGTACAGAAGGAAACACAGCATAAAAGGCTACACCATCTCTCACTAAACGCCCTCTTCCAACGGTTGAA TGAGAAGCCTAGAAATGACAGAACATATGTAAAAAGTGTTTAGGGTCATACTACATGTTCACATCTTACA GAGTGGTAACTGTAAGTCACACTGTCCCTATAGTTCTCAGCAGAAAGAATTCTAAGACATTTGAGAGAAA ACCCAAAGCAAAGGCAGTGCCCACTTATGCACTGGCCAAAAAGGGACTGTGGACAAGAAAATGTATTTCT TGTAGGAGTTACTTCACAAAACCCCAAACTTAAAAAAAAACAAAACCAAAACCAAAAAAAAAAAAAACTT AAGGAAACATTAAATGGAAAATTAGCTATGCCTGAGCCTCAGTATTCACATCCACACTTCAATACTGGAA GCTTTGCCTCACCTTTGAGTTGCAAGGCTATCATCCTGCATGGGAAAACTTTCTAATTAGGAAAAAAAGC AGAGAAAAACAAAAAATATGCTAAACTCAGATGAGGAGTTATCTAGATCTCACACACTTGTTTGGTGGAC ATTTGCTTGTCTGAGTTTCAGGCTCTACCTTCTATGCAACAAACCATGAGCAGAGAGCACACACCTTCCA CCTTGGAGAAGTTCCAGGTTCCAAACACAAAATACACAGTTTGGTTTTGGTTCTAGGTTCATTGCTTTCT CTGTCTCTGTGATAAAATACTCTGACAAAGAGTAACTTCAAGGAATCCGGGAACCAAGATGGCTCCCCCA GGTGCTCCGGCAAAGCTCCTTCTTAGAATTGGGAACAAAACACCCATGGAAGGAGTTACAGAGACAAAGT TTGGAGCTGAGACAAAAGGATGGACCATCTAGAGACTGCCACACCCGGGGATCCATCCCATAATCAGCCT CCAAACGCTGACACCATTGCATATGCCAGCAAGATTTTGCTGAAGGGACCCTGATATAGCTGTCTCTTGT AAGGCTATGCCGGTGCCTGGCAAACACAGAAGTGGATGCTCACAGTCAGCTATTGGATGGAACACAGGGC CCCCAATGGAGGAGCTAGAGAAAGTACCTAAGGAGCTAAAGAGGTCTGCAACCCTATAGGTGGAACAACA ATATGAACTAACCAGTACCCCCAGAGCTCGTGTCTCTAGCTGCATGTATATCAGAAGATGACCTAGTTGG CCATCATTGGAAAGAGAGGCCTATTGGTCTTGCAAACTTCATATGCCTCAGTACAGGGAATGCCGGGGCC AAGAAGTGGGAGTGGGTGCGTAGGGGGTTGATGGGGAGGGTATGGGGGACTTTTGGCATAGCATTGGAAA TGTAAATGAAGAAAATACCTAATATAAATAAATAATAAATAAATAAATAAATAAATAAATAAATAAATAA ATAAGCAACTTCAAGGAGAAAGAAGGTCTGTCTGTTCACAATTTCAAGTCATAGCTCATCATCAAAGGGA AGTCAAGGCAACAGACCTTAAGGTAGCTAAGCAGAAGCATAGTTAAGAGCAGAGAGAATGTGTTCAGCTG TGCTCAGGCCTTCTCCAGCCTTCTCTACTCTTTAGTCCAGGGTTTACCCCATGAACTGATGCAGCCCACA TCAGAGTGGATCTTCTCACCAGGATTAAAGCATTCGGGAGACATGCCCCCAGGGGCAAACCTCACTGATG TTCTTTCTAGGTAACTTTACATTGTGGGAGGTTAACAATTATTTAAATTAACATTTTCTGAGCAATGGAA TTGGAGATCTATTTCTTCATTTTAGCGATAGCCTTAGAGTAAAAGGAGAAACCAAGTGACCAAATGCCAT CAGCAACATCAGTATACTGTAATGAGGCTGCCTCATCCTTTAGAAATTCTAGTACATCATTTGGATTATT ATTTTATCTCACATAGAGACTAGTGGAAATATTTCTTACAAGTGTAGAGATCCATACAAAATACAAAGCA AGGATGTCTTTAAGCAAGTTTCAGTACATGTGCTGAGGATGTTCACACACTACAGCACTAAAGGAATGGG GTAAAGAAGACATTTAGATGGGATCATTTAGTCCCATTCTAGCATCTAATTATATGATGATCTAACTGCA TCACAGAAATATGTAACAGATTTTGTTTGACATTGATGAAAAGTTGTTTTTTTTTATTCCACTGTGATTT GACACCATTATAATAGTAGCATTTATGTAGCATTTTTCCATGAGTCCCAAAAATTCCACACTCCCTCAGA AATATCATGGATCAAACACTAAGAGAACCCACAGTAAGTTAGATTTTAAAACTATAACTGTTTCAATGAA GTTATGGAAATACGGCTGATGAACTGGAAAAATAGCAATTTTGAAAAACCTAACTATACTCACATGAAAG TCACTGTCAGTGTATAAAGTGGTGTCAATATGAATAGACTGAAAAAGAGAAAAGGACGTCATATGAAAAA TCTAGGTATGATACTAGCAACTTCCTGTAAACAATAAATTTTGTCCCTTAACTTAAACGTGAATTTTGCA ATCATTTTAAGCTCCTGTTATTATAAGAGAACATATTGAGAGACCGCCTGACCCTCCTCTGCATTTCCCA AGCAGGGAAATTCATATTGATGTAACATTTCTCTCTTCTCCAGATTTACCAGTTTTCATCCCCCTGTCCA ATCCTAGACCTTTAACATTTCAAGAATGTGAAAGAAATGGAGTTATATAGAATGCAACCTTTTAAGATTA GCTTTTTAAAAAATAACATTTTGAGAAGTTCTGTTATAGGGGAAAGACAGCAGAAGGGCCAAGGTTTCTT CACCCCTTAATCTGACTCCAGTTCCCTTCATGGTTAATGTGCCTCACACACCATATGTGATTGTTATACC CAGTGTGTGGACACCCCTACATAGCTACAGTGTGTGTTTAATGCCCTGAAAGTCTTGGTTCCAGAATAGA AATAGTGAGCCACTGGAGGGCAACAGTCACTGGGATTGGACTTTGAAGGACACTGTAGGACTTTTATCTC TTCATTTTTTCTTCATTTTTCTTTTCTAGAAAATATGTGAGCAGATTGTTTTGCCATTAGAAGATGGCAG TCCCACCAGAGACCCAAAAATAAATACTTTAGTATATAATATAATAATTATATAATATGTATTCTATGGT TTTTAAATATATATATATATATATATATATACAATGGCAGGTACCTAGCATTCTAATGGCACACATTGCA GTGGAACTATGTTAAAGAACACCATACTCTCCCTACTCACCCCTCCTTTTTCCGTCTGTCTTTTTTTTTC TGTAATTTTATCATCTCTCCAAGGAAAAATAGTTGGACCTGCCCAACATGAAACATGTTTAGACTGGGTT CTTTCACTGCATGGCCGGCTTTTGGGGTATCTGTTATCTGTTTCCTTTCATGCCTTAGCCACATCTATCT CTTGATCTACTGAACGGGTACACCAAAAGGAATAGCTTGGCAAATAAAAGTGGTGAGAGATTTGTTTTAC TCCCAGAGGAAAACTGTCTTTCCCTCACTGGCAAAAGATTAAGTATTTTAGTAATATTTTATATTAAGTT AAAGAAGTTGTCCTTTGTTTCTATTCTGCTGAGAAATTTTATCTTGAAGTAGCTCTACAGCAGTTTGCTG AAGCTGCTATAACAATTTCATATATGCTGGATAGCGTAAGTAAAGACATGTACTTTTCTCGAATTCTGAA GACAATCAGTCTCAGATGGAGATGTTAGGAGTGGTCTCCCAAGACTTCTCACTTTGCCTTATCAATTCTT ATCCTATCCTATCCTCTCTCTGTCTCCTCAGGGTATCTTCCTCCTGTGGATACCCTTCTTGGAGCATGGT TTCCTGCCCTTCTAAGGAAACTGGACATATTAGATTAGAATCATCCTAATGGACCTGTTTTCACTTAAAT TTCTCTTTAAAGACCCTCTGTCAAAAGACAGTCACACAATGGGATATGGACATTCATTAAAAAAGGAAAA TCCATCCTGTATAAGAGGTACTAAATTTATCATGTCTTTTCTAAATCAATTGATATGATTTTCCTTTTTG GCCCCTGATATGTCAGCCTGAGTTGACTGATAGTTCTTGAAATAATCAAAAGACTTGCATAACTAGAATA AATTCTAAATTCTATACAGTGATGATATAGGATTCTTTTTATAATACACTGGTAGATCTGATAGGTTGAT ATTGTACTGAGGAATTTTATACTAAGTACATGAGAGTTGCTTATATTTGGTTTCATTTTTTAAAATGTAT CTTTATCAAGTTTTCACATCAAGGTAAGATTAGTCTCCTAAACTGAATTAGAGGGACTTGCTTCTTCCTG GGGGGGGGGGGGAGGCATGATCAATGTTTGAAGTAAGTTTCAGGATGATAGAGCAAGGCTAGTCGCTCCC AAGTGTTCTTCTAGGAGGCTCTCCATGGTGACCTGAACAGTGTCAGGAGTTGTAGGAGCTCTTGAACCAC CAGTTTCATCATTGTTATGTTTTGGGGAGTTCATGATCTTTGAAGAATGTTTCCATTTCCTGTAAATCAT TGGTACAGTTATAAGACACTGAGGGAAACAGTGACAAATGTTATCCCCACAGATCCTGTGTCAATCGCCA CCAATTCCATCCATGGTGCCTGGTACCATCTCTATTATTGTTAGATGTTTACAAATTTTATTGATTTTTT TTTACTAGTGCCTTTATGTTTCATTGATTTGCCCCCTGACTTCCTGATTTCAGTTTCATTAATGCCTGCT CCCAGATCTATTTTTTCCTTCCTTCTACTCATATTGAATTCATTTTGTGATTCTTCTAATTTGGTGAGAC AGAACTTCAGGTGCTGGCTTGATGCTTAGTGCTTTTCCTCTTTTCTAATTCACGCATTGCATTTTTAAAG TTTCCTCATCTTTAGCAGTTCACATTGTAACAGACAGGGTTTCATTTTATTCATGTCTGTGAGGTTGTTT GCGTATCTTTGGGAATTAGTCTTTGACCCATGTGTTACTTAGAAATACGTTGTTTAATTTGCAAATTTGG GGGACTTTCCTTTTGTATCTTTTTATCTCCAGATTGATTCCATTGTGTTCTAAACATATTTTCTGCACAA TCAAAGTTCTGCTGAGTTCAATGCCCCTTACTTTATGGCTCGGGAGATATTTAGTCTTGGTGACTGTTTT ATGTGAATTTTTAAAAGTTTGTTTTGATGTCGTTGGGTAGAATGCTTCATGCATAAAGTCAAATCCTATT AATTGTATTTGTTATGGTTCCTATGTTTTTGGAATATTGCTACTTAATAGTTTAGTTAGTTATTGGGCAG GAGATGCTGAGATCCACAACTGTAATCATAAATTTGTCTGTTTCTCCTTTGAGCTGTTAAATTTTTGTGT CATGAATTTTGAGATTCTGTTGCTTGGTGCATATATATTAAAAATCAATGTTTCTTCTTCATGAATTTTC TTTTGTCATTAATGTTGCTCTTGGCATTGGCAGCTCCATTTTCCATCATGTTGGCTTATCTAATACTAAT ATAAGACTAATCCTGCTTTTGATTGTGACTTTCCTCTTCTTGGTATGATTAATGCTTTTTCCCTGTGCCT GGATATGCTGGATATTATGCTAGGAGACTCTTGTCCTACTTAAGTATTCTATTTTAATATGCAGCCAATC ACCTATTGAGATGTTTTGTGCTCAGCCTGACTTACTGTTGTGTGCTGTGATTCTAATGGCAGCTTAGCTT TCAGAACCTCTTCAAACCTTCCACTGTAATTCTCAATCACTGCAGTAAGAGTTGCTTCTTCATACCAGGT TATGTGGTTCATGGGGACAAAATATATATTTCAGGGTTGTCATTTATGATGAGATTCCTAATCTTTCTTG ATTAGATTGAGCCGAACCTGCTACTAGGCCATCTAGCAACTCTAGGCAGGAGAAGATGATCTACAATGCC CAGAAAAAAAATACCTCATTCTAGGGCTAACACCACATTGGGTCCCCTTCCTGTAACCCATTTTCCAGAT AAATAAGTTTCTGATTTGTGAGTTGGCAATTGAATTTATTTGTTATTCCTTTAGTAGTTCCTTGACATTT TTGTACACCAGTTTTGATACTTACTCTCCTTCTAACTCCTTCCATGACCACATCCCGTCCCTACCCACCA AACTTTGTGCCCTCTTCGTTTCTTCTTTCTTAATTAAGTATGATCTGTGCTGACCATATACTCTTATTAG ATGTGTGGCCATCCACGGTTGCTAGGTAACCTAACCAGGGACAACACACTTAAAGAAAACTTACTTCCCT TCTCTCAGCAACTACTAATTGCCAGCACTTCTTCAGTTGGGAGTGAATTTTATGTTATTTTCCACATTTC ATGTTAGGATTTTATTTGACTTGAGATTGTGTGGGTCTGGGGTATACTGACAAATGTGTATGTGTTCACA TGTGCAGCTGCCCTGCTGTGTTGATCAAACACTTTCCTTGGAGTCATCTACTACCCTGGGCTCTAACAAG TTTTCCAATACCTCTCCTACACAGATAGCCTAGTCTTGGGAAGAGGGTATGTGATACAGAAGTCCCATTT AGGGATGAGTGCTCTGCAGGCTCATATCCTCTGCACCTTGACTGGTTGTGGTCTCTGTGTTAATGACCAT CTAGAGCAAAACGAAGCTTCTGTGATGAGTTTTGGAAAATGCACTAATCTACAGGTATAATGATGTCATC AGGAGTCACTTTTATGCTATGCATTTAGCGAAGAAATGATAATAGGTTCTACATTAGAGTCCAGGATCTG TCTACACATAGTTCTTAGCTCTGATAAAGGTGTAAATCTTAACAGTAAATAGAATTTTAAAACAATGTCT TAGAAGGCTTTGCAATGTTCATATATTTCTTCACAAATATTATTATTTCAAATGGTATTTCATAGAAATT TGTAGAAAAACATAAGACATGCATACTTCATAGCCATTACATGTTTTCTTTCTGGAAAAACAATGATATC AATGACCTAACCCAGGACTTGGCTCTATGTGCCATAATACTAACCTACCATGTGGGATGTAGCTAGTGGC AAGGCTCTTGTAGACAACTAGCCACTTTTAAGTTGGATTGGAGAACTGCTCAATACTACTACTACTACTA CTACTAATAATAATAATAATAATAATAATAATAATAATAATAATAATAAAATTATGGCTGGTACTATATC CCTGAGCAAAACCCCATGGCTGGGCAGCCATACGCCCTAGGCCACATGCCACTGATATTTTCTCAGTGGT TTTGCTGTCAAATTACCTTCTCAGTATTTATAGTTGTGTTGGTAGACTTGGTATCCGTCGGTAGACTTGG TATCCGTCACCCTTGGTCAGAGGCATTTAGTCCCCCCCCCCAGTAGTAAGCAATCAGTGCTGGGGTATAA TTGGTCAAAGTGTTATGAAGAAGAGATTGACTGTTCAGCACTAAATAAGACATTTTTATGAACCCCACTC CTAGTCCTGCCAAGACTCTGAGGATACTGATGAAAAGGATGCAGAAAAAAAGTTAAAAACCAGAGGGTGA GAAGAAGGACAATGGAATACTGTCCTCTGGATCCAATATGGCTGTCACATGCATGAGCTCACAGTACTCT GTTATCTGTACAGATCTTGTCTGCCAAAATGACAGCTTAAAAGGGGATGTGGTCTCTTGGCCCAGAACCC CCTTAACAACCTGTTGGCATGTGATAGCTACTGAGAGAAAATGTATTATTCCCTTTTGTTGGTGTCTCAC TCTTATTTTTCTGTTTCAGTGGATGGCAAAATGGCAGTGTACATACTGGTGACATTAATTGGACTTAATG GGTTATCAAAACAGCATGAAACAAAAGATGCGAAGTTGGGGCAGACACAGTGGCAAAATAGAAGAGAGGT CTTAAAAGGAGTAAAATGGGGAGTAGATACAATCATATTTTACTACATATATATGAAATTCTTGAGAATA AAAGTTATAATACAACAATATATATCAAATATTAAAACAAACAAAAAAAGAAAAATGTGTACTCACTTGA ATAAGGCTTTCAATTTTCTCCAGGTCATATCTTACATCTATCCAGTTGGCCTCTGTTTTAGGGAGACCTA CACTGACACAGCTGTAAGAAGACACAAAACCAAGCTCTTTGGAGGAGGGTACCATTGTCTCACTGAGTAG ATAAGCAACGGAACAATCAAGATCAATTATCTTCTCACTAGGAAAATGTCTTATTCTGTCTCTGTGCATC TGACAGCCTGGTGGGTGAATGGAAATACCAAAAATACAGTATAATTTTGATAGTCTAAGCTAATTTGAGG AAGACTGTCCAACTGTCCAACTTAGTGATCAGAGAAAGGCTAAATGGATAGCTTATAAGTAATCACAGAT GTCAGGAGTTATAATGGGACAAGCTAATAATTAGCAGGTGATCTTCCTGAGATGGCCTGCTTCAGATTAT TACATAATCTGTTTATCACACTGATATTGAGATAGCACCTGATCTGTGCCATAGCTTAATGAATGTTTCT GAAGTGATTATTAGTTGTCTAGAAGTACAGGCTCCATGAAACTTTTAGGGCTAGTAAGTGTAAGATTTTG AACACTTAACACTTCCTCTTAACAACTGTGAGAGTAAATGTCCCTTTTTCATGATGCCCACAGTAATCTG ATGCTAGGATGATTTCATAGTCACTGAAGATTTCGCTAAATACAATATGGGTGATTACAAGTTTATTCAC TTTGGTTAAAACTCACATTTAATATCCCGAATAAAAGACACACTGATCACGTGACTGTGGAGTGATTCTT AAAGCCCCAGGAAAAACAGTTATTCTAAAAAAGACCAGGAACAAACCATGTCAGCCAATGACAATCTGTC TAATGAGGACAACTTACATACATGATATCAGTATATGGAACAGTTATAGCAAAAAGCTCAACTGCTTTAA GGGATAGAATACTCATATAGAATACTGCCAAGGTGCCGAAATCCTCAGTTAAGGATTTGCACGTCCCCCA AGCAGCTCCACAGTCACCTGATATCCTACGCTGAAGAGTGAAGACCTATATACAAGGAAACTGAACATAA TACAGTTTCAACATCAAGATACTCAGGGACAAATGATTGTGGCCACTAACTTGATAATAGCTATGACCAA AGATGAAATTACCCCAAAATGAAGACATGAATGCCAGCCTCAGTTAAAAAGTGACTGTTTAGAAGGAAAC ACAAGTAGCACGAGATGGATGTATTCCTCATATATGGTTTCTGGAAAACATAAATGTAGACAAAATGGTA AAACATCTCTAGTTAAAAACATGGAAGACTGAATTTTATTGTATTTTAGGTTCAAAATTGTAAAGGTGTC ATTTCACAATCAATACTCTTTATGGAAACAGTGAAATTTCAGTGGCTCTTCTGTTTTGTTTTATTCTAAT ATCCAGCTCTCTGTATGTACAATATCTCCAGTAGCCATTTCTCCAATTGAAGGTCAATGATAAACTGACA TTCTTAATTACTGACATGTATACTCTTGGGACTACTGAGCTTTTTCTGTCATTATCCTCTTCCTCTTGCC CTTCTGTCTTTATTAATAGACTACACATTATAAACAGCATAGATTAGTAATGTCACACAATAGCAAGCAC ACTAGTTTTACATTCATTTCATACAAGTATATGGAAATTAAAATACATATTATCACACAGAGAAAAACTG GATAAAATTTAAACCAATAAAAGTATTTGGGCCAGGGCTAGGATTGATGTCGTCGGACCTAGGATGAGAG AGACAGGAACAAGGGATTCTGTAAGCTCAGGACTACCCTGGTGAGTTCCAGGCCACCCAACGCTACATAG TTATGAAATCCTGTCTCAAAAAGAAGTATATCCTTTTTCATTCTAACAGCTCCTACATGAAAACTTAATT TGGTTATTTAGGACCTTATTTGTCTGAGATAGACACAGTTTTAATTCTCTGACCTGCCTTGTCAGTGCTG TCAGAGATTTTATAGTTACAGAGAGCAGCGCCAGTCAACACCTCAAACACCAGGAGTCTGTACTCTGTGT AGACTCTGCTGAAAGATGCCTAACACCAGACCTTGCCCTCAGAGACACAATGTGCGAAGTGAATAGACAT TTAGAGAACAAAAGAGACTGAGTGAAGTGTAATAACACCTTAACAATGGCACAAAGAATACAGGCATACT GTTAGTCTTGTTTTCCAAAGCCCATGCTACTTATTCTTAAAATCAAGATAAATCTTGGAGAAACAGATGT CTGTCGCAGCCAGAGATGTTTAGAGAATAATCATGCGACTTATTATAAAGTAGAACTGAGATTAAGGTGA CCTTATAAGAAAAGCCATATGCTATTTTTGTCTGCTTTTTTCCTTACCAAAATTTTCATTACTTAATGCC CAGGTAAGAGCTTCAATGTTGAAGATGAGCTGGCTATGGCGATGGGCTTCCAGCTGCCATCCATCCAGAA CTCTTCCTGCAACAGAACCAGGATGAAGGATGACTATCAAAACACAAGCGAGTAAGCCAGAAGCAGATAG AGAAACGCCTGGTGCTTATGCCACATCTGCTTTCCATAGGGTAGGCTTTGTGTTCCTTAACACACGCATC AGATTTAGCCCTCCTGAGAGCATCATTTGGAAAATCTGATCTGGCGTCACAGAAATCCCATGACTGCCCG GACTAAAACCATTTTAGTTTGTCTGAATAAAAGAACAAAGACATTATTTCACACAGTGTCCCAGATCTGA CAGGTTTCAAGGCCATCTCAATTTGAATAAGAGCAATCAAATACAAAGATTCACATAACCACCAGCTGAT AGTCGAATTTACAACAACAGCCTCATCTACAGACTTCACATAGATGTCTTAGGTATGTTCTTTAAAATAA AGTTTAATGAGAACACCATTTGTGAATTTTCTATATAACAAAATTATATTTTTTTCTAAACCAAAACAGG GGAACATGTGAAGTTTAAACACTCCCATCAAACTCCATTTCATTAAAATATTTAGGCTCAAAATATCTCA AATATGGATAGTCTTGCTGTCAAAAATGTTACGCATTAGTAAACCTTACCAATGAAAATATCAAGGTCTG TTGCTTCACTGATATAATTTATCACAATCAATTATCTCAATATTTTACACATTTCAGATGCTTCCTAATA TTAAAATATTTGCTATAAATTATGTGCAATGTATAACTCTTATTTTTTTACATTTACTATGTGTGTGTGT CTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGCTCATACACGTGCCA TGGTACATGTGTGGAGGTCAAAGAACAAATTGTGAGAGTTGCTGCTCTCCTTTCACCACTTGGGGATCTA GGAACAAACTTAGGCCATGAAACTGGGCAGCAAGGACTGTTACCTACTGAGTCATCTTGCTATCTTGTTT ACACTCTACAGTGTATATTACATCATTCTTAAGAGAAAATAAACCTAACATTTACCAGGGTATGAAACAG TACACTAGACTGTGTGGCTAGTACAATGCTTCCATGAAGTCTTCACCCCACGTTCCCGTTCCCTGTGACA CTGGCATTAGACTTCCCCCTCCAGCAGTGGTTCATTTATTAAACAGAGAGGCTGGTTTACACCTAGGGCT GACGTTTGCCCCTAAAGCCTAAGTCCCCTGAGCCCACAATGGAAAGTAAACGCAGCAAAGACTTGAGAGT CAGCCAGGGTTTCCCACTCAGCCCGCAATGACTTTTGTGAGGTCTGTTCTTAACTGCCTTGCTTTCATTT CAGTAAAGCTTGATTAACTTCTCCTCAGGACAACGTGTGTTCTTTGTCTGGAAAAGTGCTCTGACTGTAG TCTTTTTGCTAAGGACCATGGCTTTCCAGTTCACTGGTAACAAGAGCCTATTATGTTATCTCTGTGTTAT GTCTGTGTATGCTCCAGGCATGGCAACCCACAAGTGACAGATTACCTGGAAAAGGCAGAGAAATGGATTA AAATCTGCGTATGACAAGTGAGCACAGACCAGAAGGGAGCAGGGAGCTTGGAAGTATTAAGGCCAGTGGA ATGTGAAGCATGCCCTATTCGTTCCCTACATTGCAGGTCTTTATAGCAAAGTGTTATTTTAGCCCTACCT TGAGATATGGTCAGATGTTAACCATGAATGAGAACTGAAATAGTGTGAAGTTGGGGAGAAAGCACCTGAG CAAGACTATGTCCACCCAAGCGTTCATTTATGGCAGAAAAGTAGGAGATAACAGTTATCATTACTGTACA AATTAAAGTAATTTTCTACCAAGGGCCAGGTAATAAGTAGCACATACTTGGAAAGTCTTATGGTTTTTCT TGTAATTACATCAATGTCCACGATAGCTTGAAAATGGCGGCAGATGATGAATGGGGCTGCATCTCAGTTC AGCTTTGTCATCAAAATAGGTGGCTGGTGCCTCCTACTTACCAACTGAGAAAACAGATCTTCTGGCAAGC AAATATTTGCTTTGCTGATGACATCTGGCTTCTATGTGTCCCTTACTCCAGCTTCATTTAAACTTTACTT GTCTTGTAACTCATAGTGGCAGTTTTTCAGTCATTGGCAGTCTTAGAGCTGCCCTTGGAAGGCGTTAGTG GAACTCTACAGGAATCTCATCCAGTTCCCATCAGAACGAGCTGTTATAAGAATGCCAACCCACATTACTC TCTCACCATGTGCTCTCTCCTTCTTGTGTGTACCTTCACCATGGGGAAGCCACATATACCCCAACGGCAC TGCGACCAAGTGACACATTAATGAGGTCACTTGGTGTTGTCCAACACTGTGCATTAAATATGCCTCTTTC CTTTAGAGGTGTCCAGCTTCAGGGATGTTAAATCTTACAGTTTTTCACAAGATAACAACAAATGATCAGT TAAGATGTTTAATATTTTTTATATGTCATCTCTATAAAGATATGCAGGCCTGCACACATAATTTCAATAT TAACATGGAATCCTCTTTCTTAGAGATCTGTGCAGTTCACTCTTGGTTATCAACTTGATTAACCTGCAAT CAGTTAAGAAAAATGCCTATGGAAGGATCTGGGTGGTTGTTTCCTTTAAGAAGTGGGGAAGGTAGACCAG CATATATCCTTGGGGTGGCCTGAGGGAAAGCTGCTTTTTGCCTGCTTCCCTTCTCCCCCTGCTGGTGAAC ACATCTACTCTACTGTTGCCATTGCTGCTGTCATCCTTCAATGACATTGGATGCGGCTTCTTCAGTTTTC CAAAATGGACTCAAAAGGAACACTCCAGGCCTTGATTATTAGATCAGCACTTCAGAGGCATGTCGGTCTG AGCAGTTACCTCTCAGTGTGCAGGGTCCATTGAACTCCCTACTCCTAACTGTGTAAGCCAAGCTGGTAAC TCTCCTTTGTAATAGTGAGTCATTCCATCAGTTCTGTTCTGTACAGAAATCAGATGACGCTAGATCCCAA ACACTGCAGAAATTTAGGCCCATGGACCGTATTCCCTGTGTGCTACTACCTAGGCATGTTCCCAAGCATC TCAGAGGAAAGACCTGTCTCCCAGCCCTCTGCCTCATCTGGTCTTCTACCATTTTCCTCCCTCTTCTCAG GTATAATCACTCTCACAGTGGATTAAATTTGTTATGAAGAAAACAATAAACTGCATACAATATACAAATT ACGTCTGAATAAGCATGCAATAAAATTTCATAGGCAGAGGGAGAAGCAACTTTATCATGGCACAAGAATA ATAAGCAATATCAATACATGTTTCCAAAATAGAGGACGCTTTGGAGTCAAGTGGCCATATGAGGGAATAA ACTTTGCACAGAAGCAAAGAGGTTATTGCGGGAAACCACTGCACCGTGAGACTCCTTGAATGATGCCACT CTCTGAAGAGTCACTGGTGGAAGTCAAGAGATACAGTTGTAGAAGTGTGGTGCCAGGTTGCCTGTGACCT TCACAAACAACCTGCAGACTCTGACTGAGTATAGTAGGCAGGAAAAACCACGGGATCCTTAAACAGTGAT GCTGAGCAACAGTTGCACATGATAGGAATTCAATGCGGTATTTTAATAGTTCAACTATAAACACAGACCA GAAGTTTACTTCTCAGACAAACTGTGGCTCTAAAGCAACGAACAGCCCTTGTACTTGAATGAATTGTTTT ACAACTCCTGGCTATCCATGCTCACTCTTCAGGGAAGGAGAACCTGTCAGTTCTGAAAACGAGTTTAGCT GAATGCATGTGGAGGTTACAAGTTAATGATTAACTAGTAAAGTACAGGGCTGTTCTCTAACAACTAAGAC CCTTACATGCCCTCTCTCCATTTCTCCAACACACAGACCCCACAGAACCTCTACTGGGATGGCTTTTGAT TGTTGCCTTCTTGTCTGAATTTCCAAACATCTCCTGCATCCTATTTTCCTGCACTTTAACCTCCACACTG AAGCTAAACATTAATCTCTCCACAAACCTATTTTCGGTAATTTAGATATCTCAGCAGAGATTGAAATAGG AAAGATTTGTCTGAAAATTTCTTATAAGCCCTCTCCTCCTAAAGATCTCTTTTGTGTCTCCACCATTACC TTCGTATTTTTATAAAACTATTACTGTAAGAACCAAACAAGGAAAACCTGGAATGGGTTCCTACATGTTC TTGTTCCTGCAGACTGATAGCAAGACCCCTCATTCTTGATACCAAGCCCTACTGATAGTGATACCCAAGT TTCTTGTATATTAGGGGAGAATACTTGCTCATATTTTAAAACATCTTTAAATTACCCATACTAACAAATT CAGTATAAATGCTATAGAAATGAATACACTAATGGTCAAGAAAAAATAACAAAGAAAAAAATCTGTGCAT GGCCAGTGTGCTAGTTTGAATATGCTTGGCCTAGGGAGTGGCACTATTAGTGGCTGTGGGCTTGTTGGAA TAGGTGTGGCCTTATTGGAAGAAGTGTGTCACTGTGGGCGTGGGCTTTAAGATCTTCATCCTAGATGCCT GGAAGTTAGTCTTCTCCTGTTTGCCTTCAGATGAAAATGTAGAACTCTCAGCTCCTGCTGCACCATGTCT GCCTGGATGCTGCCATGCTCCCACCTTGATGATACTGGTCTGAACCTCTGAACCTGCAAGCCAGGCCCAG TTAAATGTCCTTAGAAGAGTTGCCTTGGTTATCATGTCTTTCACAGCAGTAAGACCCTAACTAAGACAGT CAAATTTTTCTGCCAAATCTATTTGATCTGTGATTATTGAATCATGGCGTTGAAAGGGCCAACATCCCAT AGCAGGTGGGAAGTGAGCCAGAAGTTCTTTAACTACTTGAGTAACTCTTTCCAGCTTCCTACAGGGATAA ACAACCAAGAACAGAGAGAATCTGTACTCCACACTAACAACAGTAGCTACACCACATTGTCCAAACCCCT AACTTCTCAGAACTTTCCAGTATTGACTTTGCACTCTGAATGGACCACTGTGCAGAAGAGAGGAGTCAGA CCCAGAACCTTATAGAGCCTCTATATGAAGCTGCACAACCTGTCAATGAGTCCTGATTCCAAAAAAGATA CTTCTGAGAAATATGTTCTAGCTAGAAATATGTGATTACGATGGCTTTGATCAAATGTTTGATGGGGAAA AGATAATTCTTGATAAAAAGAAATTACGTGGAGAACTCAATTACCAATATTTTCCATAGTTATACTGTCT AACAAGGCTGGAGAGACGGCTCAGCAGTTAAGAGCACTGGTTGTTCTTCTAGGGGTCCTGAGTTCAATTT TCAGCAACCACATGGTGACTCACAACCATCTATAATGGAATCTGATTCCCTCTTTTGGCACACAGGTGTA CATGTAGATAGAGCACTCATCTACATTAAAACAAACAAACAAACAAACAAACAAACAAATCCTGGGGCTT AGATTGTGTAATGAAAAAACTGACTCTAAGTTGTTCTCTGGCCCACAGTGACACACTTCTTCCAACAAGG CCATACCTACTCCACAGCTCCATAAGCTCCTGGGATACATATCCCATGGGCACATAAATAAATAAATATT GTCTAACAAAACTAGAAAAATTACCATTGTTAAATATTTCCCAGATTTTACATTTTCTTTTAAAAATCAT ATTATAATTGAAAATTTTTACTTTAAATTATATACAAGCATACATGAATGCAGTACCTGAAGAGTCCAGA AGATGGCATCAGATCCTGCAAAATTTAAGTTAAGGATGGTTATGAGTCACCTGACCTGGGTGTAGGGACC CGGATGTGGGTACTCTGCAAGAGCAGCAAGCACTCTCAGCTTCTGAGTCATCTCTCCAGCTTCCTCATTC CCCAAGACACACATCAAAATATTAGAAGTACATAAGCAAACAGCAAATCTGAGTATTGGTTTATTAGTAA CTTATATGTGGTGGTAATAAATAATCAGTTTTACTTTTGTTGGCATCTGAACAAATATTCAGACAACTAC AAGTTAGCAAAGTGTTTTTGAAAGGGAAAGTACACCCAAGAGCAAAGTCCCACATTAATAACCTCTTCCA TACAGAAGAGCAGTTTTATTGTTCCAATGCTGTTTAGAGCTGGAAAAAAAATGTCTGCAAGACTCATATC TTATTTGATTTTACTTCAATAAATTTTTATATTAAATTGAAGTTTGTGGACAGTACACTGCCAGCAGACA ACAATGACACTTGCCCTCAGCATCCACACAACTGATCGATGTTGGAGAATGGTTCTCTGCCTATAGAGAA ATCTCCTTGCACTAACAGCCCTCAGTCAAGCTCCAAATGACTTGGCTGGAGCGTGCATGGAAACACTGCA CGCATGAACAGCAAGAGTCTTCTGGAACAGTTATGGACCACTCAAGTGCTTGTCTTGTCTTGGGAGAAGT GATTTCGTAGCTTCCAATAGAGACAGACAACATAAAACTTATAAAAGGGTAAAAGACGTGGTACGAATCT GGTTGCCTCAGCTTAGCCCTTCAGTCACTCAGGACTGTGCCTGACTGAAATCTTATCAAGCAAATGACAA GAGGAGAAATGAAAATGTGTTTCCTGGCCATAGATAGCATTTGAGTGTCAGCACTGCTCATGGCATGGAT GTACAAGGAAACAGCTTGTTGGGGTCACTAATTACTCTTGACTTATTTTCTTATTTTTAAAGAGAAATGC CTCTGTTGTTGGGTTTCCTTTTGAACAGCAAGTGTCTTGGATGGTTTTATGTCACCTTGACATAAGCTAG GATTATCAGAGAGGAGGGAACCTCAGCTAAGCAAATACCTCCATAAGATCAGAATGTAAGCAAACCTGTA GAGCATTTTCTTAATGTGTGATTGATGGAGGAGGCCCCAGCCCAGTGTGAGAGGTGCCACCCATAGACTG GTGAACCATCGACTGTGTTCAATGAGAACACAGGATGAGCAAGCCATTAGCAGACCTCTTCTACGGCCTC TACATCAGTTCCCACCTTCAGGTTCCTGCCTGGCTTTGAGCTCCTGCCTTGGTTTCCCTCTGTGGACTGT GATTCGGGATATGTGAGCCAAAGAAACCCTTTCCTCTAAGAGTTTCTTTTGTTCATGATGTTTCATCCCA GAAAAAATAACCATAACTAAGACACCAAGCAATCTGTTCCCAGACCAAATGAAAGATTGCTAAGAATTAT GCTATCCTTTAAAAACGTTTCGGCTCCTTTTTGTAGAAGTTAATCAAACAGGCCAACTGCCAAGAAAAAC TGAAAGGAATAAAATGTTTCATGACTCATTTTATAGAAGAATTTAAAGCATCAATACAAAACTGTTTGAA GGAAGAAAAGTAAGGAGGGAGGGAGTAGGAGAGAGTAAGAAAGGAAGGTAAGCAGAGAGAGAGGGAAGGA AGGAAAAAGGAAGGGAAGGAGGGAGAGCAGAGAGTAAGAGAGGAAGGAAGGTAGAAAGGAAGGGAGGGAA GGAGAAAAGAAGGGAAGGAAGGAGAAAAGAAGGGAAGGAGGGAGGGAAGGAAGAAGTGAGGGAAAGAGGA AGGGAGAGGAAAGAAGCAAAAAGGAGAGAGGAAGAAAGGATGGAAGGTAGGAAGAGAGGGAAGGAAGGTA AAAGAAAGGAAAGGAGGGAGGGAGGAGGAGAGGAACTATGTTGTAAGGGATATAGACACTTGGTCATACA TCATTCAATTAGTATATAACATAACCACTATGTTGCTGAACTGTAGGACATCAGGTGTGCAGTGGCTCGT CCAGATATCACCAGTACCAGCATGTTAAGAGAGCAAAGCTGTGGCCATTCAAGGTCAAGAGAGGGTCTGT GAGATAGATCTGGGAGTGAGGGTGGAGCATGACTTTGTGTGTGAGAGACAGGGAGTGACAATGCAAGCAA GCCTGGGTCCGAGCTTAGGCATCTGGATCCACGTGCTTAACGGAACGGTTGTGGATTTTTTTTTTAAAGA AAGAAAATATTGGAACAAGAATTTGTAACATGGCAGAATTGAAATGAAAATATATTTGCCACAGTGAAAT AATCTCCAAACCACCTGATAGATATTGGGGACTGTTTCATAGGAAGCACGTCTCAGCAGCTAGGTCTGGA GTCCAAGGGGACATGTTAAATGATACGTGGTTATTGTCATTTCAAATGTCCAAGTAAGACACCCAAAAAG GCTTGGTTGGTTTCCACGTACAGGGAGGAGAGATGCATAGACTGTGTTGTTCTTTCCTACAAGAGGCTGG TGGGGGAACAGTTCAGAGAGAGAACAGTGGGTGCTAAGATGAAGGCTATGTCCAAATACCCAAGTCAAAC ACGCCCTCAGTAAAAATAGCATGGGGAGCTTTGTTAGTTCATCGGTAGCTAACAGGACGGCAACACATAA AGATGCTTTCCGGCCAAATCTTATGAAAGCTTACTTTATAGGCAAGGCATAAAGCCATCTCCAGGAGGCA GCTTTACGAGAGTGCTCTCCAGCAAGTGTCTGACAAAAGCAGCCATCTTACAAGTCTCAGATAGTTTGAA CAAAAGAAACAAAACCAACCGGCAACATAATATTTACCCCATAAACGCAAGCTCAGAGACAGGGAGGGCA CACTTCTGAGCATGGGACTGGTGGCCTGTCACTCCCTGTCTCTCACAAACAGAGTCATGCTCCTCCCTCC AGCTACAATAAGGAACTGCTATCAAACTGGAAAACCAACACATCGGCTTCCAAAACACATGGCCTCCATG GTCCCCCCTAACAGAACCCTACAGCTTTATGAAGCCACGCACTGACAGAACAGCAATGGAAACAGAAAAT AAAATCAAAATGAAAACATAAACAGCTGTTTCCCTTGCACCGTGGCAACACTGCAAATATATAGCAGTAT ACGTGCCTGCCTGGTGGAGGCTTCTTTTGAATTTTTTGGAATGCCAAGTGGCAAGGCAAATAGATTCACT CTCTGTTGGTTGTTATTGTCCTAATTTTATTGCCAAGAAAAAACAATCTTTCCCTATGTGCATGTTACCC TTAATATCCTCTCTTCCGGTGAGAGAAGTTATAGCTTAGGCCCAATTTATAATTTTACTGTTCATTTAAT TTCCTTTAACACTTTAAAATGTTATGTGAAGGCTCTGACTTGTCAAAACTGAGCCATCCATTTCAGGCAT AACCCATCAAAATGACAACACGAACAGTTCTCATAATCTACAACAGTGAAAAAGGCAGACCCAATGCTTT AAAAGAGAGAGTTTGTCCACACCAACTACTCTGCACTATCATGTTTGACATTCGTGTGTTATTTTAATAA TCTGTGCTGGAGAGCAGAAATATTTGTGCCTCATTTAAAATGCTATCAAGTATAAAGTTTTTTTGTTTTT GTTTTTTAAGTAGCATTTGAGATAAATATATTTCTGGCCCAAAGAATAAAGAAAGATGTCTTTGAGAATA CAAAAGCCTTTATTTCATAAGAAAAAAAATTATCTCTAAAGCTTCCCCACAGCCGGTCCTCCAGACTTTC CACCAGGCTTTGCTTGAACATGGACGAAAGTACACACACCTGGAATTCCTTTTTCCTCTTTTTACAAGAT CCAGGAAGTGAATTCTTATTAGTCAAACCCAACTCAAACACATCATAAATGATACTTGTATCATTTATGG AATTTGGGAACTATGCATATTTATAAGCATGAATGCTGCAGCCCAGTGATGTCTATTGGCTTGCCCCTAG TGAGTTATCAAGTAATCGAGCTTTAAAGCTGTTTAGCCCATAATCCACACCATTCAAAACACACAGAAAG TGGTTACAGTCTGTCAATGGACTCTCTGGCCTGGTGAATACCAGGAGAAAAGGTGACTCCCTGGGTATTC AGCATAGAGACTAAGAAGCTGTCAGTTAAGAAGGCAATGGTTCTATCAAAGTGTTATATTCTACTGCCCA AGAGATGCGCGCCATCTTTATCCTATTTAAAATCAGTAGAACAACGCTTGTATCAGCTTCTCCTTTGTGG GGACATTAAATCTGTTCATGTAAATGTTTGTGATCTCTAGTTAATTATCTGTGGGTATTTTATTATCCTG GGTATAATTTCCCTTTTCTCTTCTATACCTTTGTTTTCTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT GTGTTTTAAGACAGGGTCTCATTCTGTAGCCATGCTAGCCTGTAACTCACTAAGAAGCTTAAACTAAGGG CAACCCTCCTGACTCAGGTGCCCAAGTCCTTGGATTACAAGCATGAGCCATGATGCCTCCTTGGCTCTTT CTCAGTTGATTTTGCATCTTTTGCAGCTCCCCTGATGCCTTATTATGAGACTCAAAAACATTTAACACAA TTAACTGGGAAAAAAGGCAAGTAAGTAGAAAAACTGTAATTTCCATGGGTATTTGGACATGATGCTAGTT AACCAAATTTCTTCAGTGGGTTTATGAGACAGAGGGGAGAAGGCAGCATCAAAGCAGGTGATAAAATAGG GCAGATTACACTAACAAACACAAGCACAAATCAAAAATTATGTAGAGCATAACGAAGCATCATTTATGCA CTGCTTGACCATCTGGACCTGCTGTTTTATGGGACAAGGGGGTGAGAACTTGTGCTTGAGCCTTCTGAGG TGGTACTTTTTATAGCAGTATTTGAGACCCACACAGACTGGCTGTACAATGATATGGCTGCTTTACAGAA GGCCCATCCATAGAAATCAAGCCCTGCAATGAATGCTGTGTAAGGACAAAGCCCCAGAGTTCAGTCGGCT GCAGACTTGCTGACATTAATTCTGCCAATTCCTTGTGATTCATAGATTTATGCTTCTCCCTTCAAGTGGG AGTATGACAGGGGGAGGACGCGTGTTGAGATATCGTCTCTGTGCTGTGGTGGGTCACTTAGGACTATGTT GTGTCATACTGAATGGCTTACCAGAGAAGTTGCTGAGCACAAACATCAAATATGACATTAGTTATAAAGA AGAGAGGAAGAGGGAGAGGGAGGGGTGGGGGAGGGGAGGGAGGGAGAGAGAGAGAGAGAGAGAGAGAGAG AGAGAGAGAGAGGGTCGAAAAGGGGAAACGACTGACAGAAATTATGAGGAAAAATTAGACTTAAAATTAC ATATACTATATAAATCACCAACAGTTTGAAAAGGATGAGAGCTATGAAACACAGCATCCATATTTCCAGC ATGATAAGCAGCTTTTGCTGATAGCCAGTGAGGAAATATCAATCTCATTCCTAACCTACAAAGACTGAAT TCTGACAACAAACTGTAATGAGCCAGGCAATGGATTCCTTCCAGCATCTCCAGATAAGAGCTTCCCCGGT AAATGTCTTGCTTTCTACCTAATGAGACTCTGGAAAAAATATCCAATTGAACTTGACACCCATGTAAACT GTGACTCTATTGAAGATGTTGTTTTAAGCCTCTTTGTTCATGACTTCTTACACGGCAATAGAAAGGTAAT TTATAGAGCAATGTGTTTCACTGCAGGCATTGTGGAAAATAGATATAAACTAAATATTATGTTTATCTAT TTTATAAATAAATATATACAATACATATATTATATAGTTGCCTTAAATGATTTTAATAGGAAGCAATGAG ACTACATATGCAATCAATCTAGAGCAGGTTTTTGGGTAATAGTTATTTTTAACGTGTTTTTCTTTCATCT TTGGGGTTATGTCATTTATCCTTTCCCATCCTCCCTCAGCCCTTCTATACACTCCTCCTGCTCTCTCTCA AAGTTATGGCCTTTTTTCATTAATTGTCATTGCAGGCATACGGGGGGGGGGGAGGGAGGGAGGGGGAGAG AGAGAGAGAGAGACAGAGAGACAGAGAGACAGACAGACACACACACACACAAACAAACACACAGATATAT ATTACAAAATATAACCTACTCAGTCTGTATAATGTTACTTGTATGTATGTCTTCATTACTAACCATCTGG TATTGGATAACCATTATTGTGCTCTTACCTGGAGAAGCCTATTGCTGCTACCCCAGCTTTCCCTATGGTG ATGAGGAAATATATCAAAGGCTTAAGGGAAGACTTTGGTGACTATGGTTGCCTGATAGTCTAGGGAATTG TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTCAAAATTGTTCCCTTG GTTGTATAACCTGAGAAGTGACCTTATGAGTGTTGCTCTCCAAAAGGCTATGTTATATATTATGAGGCAG AACACTTCATAAATTGCATGTGCACACACACACACACACACACACACACACACACACAATCATTGCATGC TTCTTGTTTTCAATGCCCAAATTCATCCGTACTGATAAGAAAGAGGGATTCTACAGTACCATGTGCTTAC ATGTGAATATGTAAAAGAAGGAGGGTAACTTTACTACAATTTATTCCAGATAAAATTAAACATCAATTGC CCACTGCAGTGCTCTAACAACAGTGGGAGGAAATGATACAGTCATGAACAAAGACAAGGTCATATTTGTC TTCTCTGAGCTAGCGCTCTACAGAAGAGAGATGAGCCTAGCAACTGCAGATAGTAGTCTATATTAGAGGA AGGTAATGTGCTAGCATATAGAGGAAGAGGTTTCGCTGGGGGAGGCTTTCTGCAAGAAGCAATGGGAGTG GACCTAAGATCTTCAGGGCGAGAAAACACTTATTGAGAGGGTTTCAAGCAAAAGTAAACTAATCATTGGC CTTGTTATAGGGGCAGGAAAGTGACAGCAACCTAGAAACAAAGTTTACTAATGCTTACTAACACTGAAAA TGGTGTAATGATCAGGACTTTGAAATGCTCAACCTGCTGGACTTTCTCACAGGTGTAAACCCTTCCCTCA TGACACACCAGACAGTAGCTAACCAAAAACTCGTGCCAGCAGCAACTGCAAGCAGACTGAGCTGAAACTT GCTGGCCTGCTTTCCTTATTATTATCTACTACTTGTGGTTACGAAATAGGAAGAATACAATGGTTGACCA TCACAAATAAAAATGAATTTACCCTTTACTACATGGTAAATTAAAACCTGCCCCACATGTATGTGGGCTA AGACAGTCACAACAATCTTACTTATTTTGTGTGCCTTTCCAAAGTCCCTTTACTGATGTGGGGTTTCTAC TTTTCTCCTCAAATCAGCACCAAGTCCTGACCATACATGCGTAGTTATGCCTGAAAATATGTGTGAGTTC CACGGCTAGGACTTAAAAGGTCTGTCCTCTCTGTTCCTTCTACTGGCACACAAGAAAACTCAGCGAGACA ATAATGATTGAAAACAGTATAGACGTTTATACCTGCCATCCCAAGACACGGAAATGAAAATAAATCAAGG AATCAATCGATAAAACTATGAGGAATAACAAAGTATATCTAAAATAACTCTATGTGACCTAAACTGAGCA TCTATAGATACAAAACTATGTACTATAAATGTTATTAATTTATCTGAAATAAAATATTGTCTTTAAAAAC TTCTGGGTTGTAATTGGGGAAATTTTGATCTCTATAAATATCTCTTGAAAATAAACCTATCCTGGCTAGC CTTATAATTAATAAAATCATGCCAACAAAATATATTTAATCTCTAATATCTATGTGATCAAGGGAACAGG ATGGACTCATTATTCATGGCTAGTTTAAGAAAGTCCTTGGTTAGAATGGACAGAATACCAGAACTCAGAT TTTCTTCATTTGAATATTATCTAAGATGTATTTGCTTTGGTCAATATTTAAAGAATGTAACAATTTCACT TTCCATATGCCAAATCATCACAAAGTGTTGGTGTCAAACTGTTTCCTAAAGCTAGTATAATTAATTTTGT CTTATACTATAAAACTCTTGTAAATCAAAGGTATCACTCTTTGTTCTTGCAATATCTAGGAAGTCAAGAA TTATTCATAATTACTTCCATGGACCTATCAAATTAGGAATTTTCTGATATAATTTTGTTAAAATAAAATC AACATTTAAAAAGTCTGGATTTTGTTTAACTTACTTTATAGTTGATTGACAGACGCCTTTAAAAGATGCT TCAAGGTGCTGACAAGTTAAGGATAATTGAGAGTCTTCTTCTGGACAGCCTTGTGAGAGAAGCTAGCAGT GGCTATTGAATGCTCATCCCTGAATGGAACATCTACATTACACCCACCAAGGCCCACGATGGTAACCAAG AAAGGATGGAAAGGCTGTAAAAGCCAGATGCAAGGAAGGAATGCTGAGAAGAGTGGTGTCTTCTGGACAT GGTCTGGCTGTCACTGTCATGGACACATAGTGTCTGTGGCTATGTGCTCAAGAAAAACATGAAAGCAGAA ACATGGATGGTTGAAAAGAAAAAGGCCTGTGTGTGAGTAAGAAAGGCATTAGAGAATGTATTGGGGAATG AATATGATCACAATAAATCGATGGTGTGCATGTAATTGTCAAAATGAATAAAATTTTCTTTCAACTTACA AATATTTATATACTTTAGTGATAACAGTCAATTTTAATATTTTCATGCTATAATTCAACTTCTGTTACTG TGTGGTTACAGCAAAGATCATTTTCTCAGTCCCAACATACAGGTATTTCTTCCCATCCCTGAAACCAAGC AACTCCACAGGAGACACCAGTTAAGTATTTGATAATTCATTATACGCCTAAGGTTGCCTCTTTGAGAAAG TGTCAGACCTGGTGTATTGGTGGCTTTGTTCCACAACTGTACCCCACTTCTGACACCTGAGACACGTCTG CCCTCTGGAATTCCTGCTGGACTGCCTGCAGTTAGAGCCCAGCATCCTTTGTTAGCCCTATTAAATTGCT ACAGCATTATTCAAGATGCCATGGGAACACATTTACTAGTTTGTTGAGAAGGATATTTTTAAAGAACAAA CAACCATTTGAGCTACTATGATAAGGCCTGGTAGGGGCCAGACACAGGAGCTTCCATCCCTGTGTAACTG ATATGTGCAACTCTCCTGGAGTGAGAACAATTCTCAGCTTGCACATGTTCTAGAAAGTTCTCTGAACCCA TCTCTATGGCTATTTTAAAGGAGATTTGTTTACATAAGCAAGTCTGGAAAACAGGAACAGCATGGAAATT GCTAACATACTAGATGAGACCTACCATACTAGGTGAGACCTACCTACGCTGTTGCACCCAAGGCTTCTTG TCCTGCTGAGTAATACTCTTCCTGTTGGCTAACTCACAAGATTCTTGCTAAAATGAGATTTTGTGAATTA CAATCAAGTAAAGCAGGCAAGAGACCTCCTCTAAGACCAACTTCAAGAAGACAGTGGAATAGAGAAGCTG GAAGCTGTTTTTAGTTGCCATGATGTGAATTGGGGAAGAGGAAAGAGGTGAGACTATCTTTAGATCTATG ATTGCCATTTAGGAAGAGAGAAGGGAATGGGGAAAGGAAGGGCAGGACATGTTAGGGAGCAGTTGCAGTC AGAAGCACTCCACCAGCAGAGCAGCCAAACCTGCCTACATTGCCTCCACATGGCAGCAAACATCAATGCC AAGTTATCTAGTAACCACAGTAACCTCACTTTTTAATTCTCCTCTGTACAGAGACTAATCTAGATCCTGA GATGTACCATCTCAGTCAAGGACCTGATCTCCTACAGTGGATACAGTAGATGACAAATCGTGGTTTCCTG TGCCAAATTAGGTCAAGGTCATTTACCTTGTTTTGTTCCACATTCTTATTTCTGAATTACACTTAAGTAT AAAATTACATCATATATATATATATATACTTATATTTTAAATATAAAATCACAATATTTAATGTTAACAT ATGTATTAAATATGCTACTTTTACTTTTAAATATATATATAATTTATTTTAAACTATATATAATGTATTT TACTTTAAATTATACTCACAACTTAAAAAGTAGTACATATATATATATATATATATATATATACACACAC ACACACATACATATATATTAAAATGTATACATTAAATGTGAATATGAAGAATGACTCTTTAAGGTGACTT TCTAAGGCAGGTCCACTCAGGAGTGCACAGGCTACAGAAGCAGAAAAACTTCTGGAACAGGGTCCTACAG TGTTGCTCTGCACCTGGGAGATGGGGCTGTTCCGCAGCCCTCTGTATACTGGTCTTGTAGGGAGAGAGCT GGTCTCCTAAGAGTGCTGACACGGGCTTAAAGACTCACAGGAGGAACAAGCTCCAGGCAGAGACAGCAAG AACATCTAACACCAGAGATTACCAGATGGTGAAAGGCAAACACAAGAATATTACCAACAGAAATTAAGAC TACTTGGCATCATTAGAACCCAGTACTCCCAACAAAGCAAGTCCTCGATGCCCCAACACACCAGAAAAAC AAGATTTAGGCTTAAAATCATACCTCATGGTGTTGGTAGAGGATTTTAAGAAGGACATTAATAACTCCCT TAAAGAAATACAGGAGAACACAACTAAACAGGTAGAAGCTCTTAAAGAGGAAACACAAAAGTCTCTTAAA GAATTACAGGAAAACACAACTAAACAGGTGAAGGAATTGAACAAAACCATCCAAGATCTAAACATGGAAG TAGAAACAATAAAGAAATCACAAAGGGAGACATCTCTGGAGATAGAAAACCTAGGAAAGAAATCAGGAGC CATAGATCACCAACAGAATACAAGAGATGGAAGAGAGAATCTCAGATGCTGAAAGTTCCATAGAAAACGT TGACACAACCATCAATGAAAATGCAAAATGATCCTAACTCAAAACATCCGGGAAAGCCAGGACACAATGA GAAGAACAAACCTAAGGATAATAGGTTTAGATGAGAAGGAAGAATTTCACTTTAAAGGGCCAGCAAATAT CTTCAACAAAATTATAAAAGAAAACTTTCCTAACCTAAAGAAAGAAATGCTCATGAACATACAAAAAGCC TACAGAACTCCAAATAGACTAGAACAGAAAAGAAATTCTTTCTGACACATAATAATGAAAACACCAAATG CACTAAACAAAGATAGAAAGATAGCAGTAAGGGAAAAGGGTCAAGTAACATATAAAGGCAGGCCTATTAG AATTGCACCAGACTTCTCACCAGAGACTCTGAGAGCCAGAAGATCCTGGACAGATCTCATACAGACACTA AGAGACCACAAATGCCAGCCTAGTCTACAATATCCAGCAAAACTCTCAATTATCAAAGATGGAGAAACCA AAGCATCCCATGACAAAACCAAATTTACAAAATAACTTTCCACAAATCCAGCCATTCAAAGGATAATAAA GGGAAAATTCCAACACAAGGAGGGAAACTATGCCCTAGAGAAAGCAAGAAAGTAATCTTTCAACAAACCT AAAAGAAGATAGACACATGAACAGAATCCCAAATGTAACAACAAAATTAACAGCAAGCAACAATTACTTT TCCTTAATATCTCTTAATATCCATGGACTCAATTCCCCAATAAAAAGACATAGAATAACAGATTGGCTAT GTAAACTGGACCCAACATTTTACTGCATACAGGAAACCCACCTCAGAGACAAAGACAGATACAACCTCAG AGTAAAAGGCTGGAAAACAATTTTCCAAGCAAATGGTCCCAAGAAACAAGCTGGAGTAGCCATTCTAATA TCGAATAAAATCAATTTTCAACCTAAAGTTATCAAAAAAGACAAGGAGGAACACTTCATGCTGATCAAAG GTAAAATCTACCAAGACGAACTCTCAATTCTGAACATCTGAGCTCCAAATGCAAGGGCATCCAAATTCAT TAAAGAAACTTTAGTAAAACTCAAAGCACACAGTGCACCACACACAATAATAGCAGGAGACTTCCACACC CCACTCTCATAAATGGATAAATCATGGAAACAGAAACTAAACAGAGACACAATGAAACTAACAGAAGTTA TGAACCAAAGGGATTTAATAGATATCTATAGAACATTTTATCCTAATACAAAAGGATATACCTTCTTCTC AGCACCTCATGGCACCATCTCCAAAACTGACCATATACTCAGTCAAAATACAGGCCTCAACAAATATAAA AATGTTGAAGTAATCCCATGTATCCTATGAGATAACCACAGACTAAGGTGATCTTCAATAACAACATAAA TATTAAAAAGCCTCCATACACATGAAAGCTGAACAACACTCTACTCAATGATAACTTGGTCAAGGAAGAA ATAAAGAAATTAAAGAATTTTTATAGTTTGATGAAAATGAAGCCACAACATAACCAAACGTAAGGGACAC AATGAAAGCATTCATGAGAGGAAAACTCATAACTCTGAATAACTTTAAAAAGAATCTGGGGTGAGCATAC AATAGCAGCTTGACAGCATACCTAAAAGCTCTAGAACAAAAGGAAGCAAATTTACCCAAGAGGAGTAGAT AGATAAACCCTTAGCTAGACTAGAGGGCACAGGGAGAGTGTTCTAATTAATACAATCAGATATGAAAATG GAGACATAACAATAGAATCTGAGGAAATAAAAAAATCATCAGATCCTACTACAAAAGCCTATACTCAACA AAACTGGAAAACCTGGATAAAATGCACAATTTTCTAGACAGATACCAGTTGCCAAAGTTAAATTAGGACC AGATTAGTGATCTAAACAGTCCTATGTCCTCTAAAGAAATAGAGATGGTCATTAATAGTCTCCCAAACAA AAAAAGCCCAGGACCAAAGCAGTTTAGTACAGAGTACTATCAAACCTTCAAAGGAGAGCTAATTCCAATA CTTCTCAAACTATTCCACAAAATAGACACAGAAGGTACTCTACCCATTCATTCAAAGAAGCTACAATTAC TCTGATACCTAAACCACACAAAGACCCAACAAAGGACAACTTCAGACCAATTTCCCTTATGAATATTGAC GCAGAAATACTCAATAAAATTCTCACAAACCAAAACCAGAACACATCAAAACAATCATTCATCATGATCA AGTAGGCTTCATCCCAGGGATACAGGGATGGCTCAACCCACTATATAAACAAACTCAAAGAAAAATTCCA CATGATCATCTCATTAGAGGCCGAGAAAGCATTTAACATAAGCCAACACCTGTTCATGATAAAAGTCATG GATAGGTCAGGAATTCAAGGCATATACCGAAACATAATAAAATCAATATACTGCAATCCAGTAGCCAACA TCAAACTAAATGGAGAGAAACTTGAAGCAATCCCACTAAAATCAGGGATTGGACAAGTCTGCCCACTCTC TCTGTACCTATTCAATATAGTACTTGAAGTCCTAGCCAAAGCAATTAGACAATGAAAGGCGATCAAAGGG ATATAAATTGGAAAGGAAGAAATCAAAATATCACTATTTACAGATGACATGATAGTATATATTAGACACC CCCAAAATTCCATATGAGAACTGCTAATCCTGATAAACAAATTTAGTGACATATATGGATATAACTCAAA CAAATCAGTAGTCGTTCTCTACACAAAGGATAAGGCTGAGAAAAGAAATTAGAGAAACAACATCTTTTAC AATAGTCACAAATAATATAAAATACCTTAGTGTGACTCTAACTAAGCAAGTGAAAGGTCTGTATGATAAG AACTTCAAGTCTCTGAAGGAAGAAATCAAAGAAGATCTCAGAAGATGGTAAGATCTCCCATGCTCATGAA TTGGCAGGATTAATATAGTCAAAATGTATATCTTACTAAAAGCAATCTACAGATTCAATGCAATCACCAT CAAAATTCCAACTCATTTCTTCAGATTTAGAAAGGGCAATTTGCAAGTTCATCTGGAATAACAAAAAACC TAGGACAGCAAAACTATTCTCAACAATAAAAAAAAAAAAAAAAAAAAAAAAAAACCTCTGGTGGAATCAC CATCCCTGACCTCGAGCTGTACTACAGAGCAATTGTGATAAAAACTGCATGGTACTGGTACAGTGACAGA CAGGTAGATCAATGGAATAGAATTAAAGACCCAGAAATGGACCCATATACCTATGGTCACTTGATCTTTG ACAAAGGAGCTAAAACCATCCAGTAGAAAAAAGACAGCCTTTTCAACAAATGGTGCTGGCTCAACTGGCA GTTATCATGTAGAAGAATTCAAATTGATCCATTCTTATCTCCTGTACAAAGCTCAAGTCTAAGTGGATCA AGGACCTCCACATAAAACCAGAGACACTGAAACTTATAGAGGAGAAAGTGTGGAAGAGCCTCGAACATAT GGGCACATAAGAAAAATTCCTGAACAGAACACCAATGGCTTGTGCTGTAAGATCAATAATTAACAAATGG GACCTCATAAAATTACAAAACTTCTCTAAGGCAACGGACACTGTCAACAAGACAAAAAGGCCAACAAAAG ATTGGGAAAGGATCTTTACCAATCCTACATCAGATAGGGGAGTATTATCCAATATACACAAAGATCTCAA GAATTTAGACTCCAGAAAGCCAAATAACCCTATTAAAAATGTGGTACAGAGCTAAACAAAGAATTCTCAA CTGAGGAATACTAAATGGCTAAAACTGCTCAATATCTTTAATCATCAGGGAAATGCAAATCAAAACAAAC CTGAGATTCCACCTCACACCAGTCAGAATGGTTAAGATCAAAAACTCAGGTGACAGTAGATGCTGGCCTG GATGCGGGGAAAGGGGAACACTCCTCCATTGCTGGTGGGATTGCCAGCTGGTACAACCTCTCTGGAAATC ATTTTGGCAATTCCTCAGAAAATTGAACATAGTTCTACTGGAAGATCCAGGAATACCACTCCTGGGCATG TACCCAGAAGATGCCCCAACCTGTAATAAGGACACATCCTCCACTATGTTCATAGCAGCCTTATTTACAA TAGTCAGAAATTGGAAAGAACCCAGATGTCCCTCAACAGAGGAATGAATACAGAAAATCTGGTACATATA CCTATTGGAGTACTATGCAGCTATTTAAAACAATGAATTTATAAAATTTATAGACAAATGGATGGATCTG GAGGATATCATCCTAAGTGAGGTAACCCAGTCACAAAAGAACACACATGATATGAAATCACTGATAAGTG GATATTAGCCCAGAAGCTCAGAATACCCAAGATACAATTTGCAAACACATGAAACTCAAAAAGAAGGAAG ACCAAAGTGTGGATATTTCGATCCTTCTTAGAAAGGGAAACAAAAAAGGGAAGGAATTGCGGAGACAAAG TTCGGAGCAGAGACTGAAGGAATGACCATCTAGAGACTGCCCTACCTGTGTACCATAAACAACCATCAAA CCCAGGAACTATTGCAGATGTCAACAAGATTTTGCTGACAGGAGCCTGATATAGCTGTCTTCTGAGAGGC TCTGCCAGTGCCTGGCAAATACAGAAGTGGATGCTCACAGTCATCCATTGGATGCAGCACCGGGTTCCCA ATAAGGAGCTAGAGAAAGTACCCAAGGATCTGAAGAGGTCTGCAACCCTATAGGAGGAACAACAATATGA ACTAACCAGTACCCCCTCCCCCCAAGCTCCCTGGGACTAAACCACCAATCAAAGGAAACACAAGGTAGGA CTCATGGCTCTTAGCTGCATATGTACCAAAGGATGGCTCATCAATGGGAGGCGATTCCCTTGGTCCTGTG AAGGTTCTATGCCACAGTATAGGAGAATGCCAGGGCCAGGAATCAGGGTTGGAGTGCAGGGGGCATGGGA GGGGATAGGGGAATTTTGGAGGGAAAACTAGGAAAGGGAATAACATTTGAAATGTAAATAAAGAAAATAC CTAATAAAAAAATTAAAATAAAAAAGAATGAAAAACAAAACACAAAGCACATATATTAAATCTGCAAACA TTAAATATTACTCTCTGATCCTTTACTTCCAAAAGGTTTGTAATTTCCTGACTTTGAAAGAATTCTAATT GGATAACCCACTACTATCTCAAATACAATAAATCTAAAGTAAAAATATTTTCTCAGATACAGCACTCTCA TCCTGCAACTCTTCATCTCTCCTAAAGCCAACAGAGTACAGGAATTCATCACCCATAAGTACCCTAGGCT GGAGACTATTTGCTCTCAGATCAACTATTCTTACCTATCTTGTCACCATGATACTGGAAGCCACACAGCC TGAACTGAGCACATCTTTCCATTGGATCCATGCAACTGTCTGTTGCTCATACACATGCTTATGCTGTGTG CTCCTGGTTGTGGGTTTCAATCAGCATCTCTTAGTTAAGTAATGACAGTAATATGTTCAGGTCTTCTTGC TAATCTTTGATTCTAATGCACTTTCAAAGTCTTGCTCAATTCTGCAACTTATTTCCAGCTTCATTTCCAT TTAGTTTCCCTCAGGAAGACACTTGTGCCCTTCACTTTGGATTTAAGAATGATGTAGCCATATCCTCTTT GCATTTTGCATCTTCAAATATAAAAATCAGAGTATTTTGCTATTACTGATGACATGAATAATAGGTAAGC ATTATCAAGACTTCTAATCTTATGTTTATATGTTACTAATTTCTGAATCACAGCAACATTGATAGGAAAC ATTATCATCATGTTAAATGTAATAAAGCTTCAGTACAAAGAAGTTGCATGTTGTCTTATACGATTGACAT GATAAAATGCCATAACCCAGACAACTTGGGAAAGAGAGGGTTTATTTTGCTTACACTTCCACACCACAAT TCATCACTGAAGAAAGCAGGACAAGAACTCAAAATGGGCAGGGACCTAAAGACAGGAGCTGATGCAGAGG TCATGGAGGGGTGCTGCTTAATGGCTTGCTCCCTATGCCTTGCTCAGCCTGCCTTCTAAGAGAACCCAGG ACCACCAGTTCAGGAGTGAACTCACCCACAATGAGCTGAGCCATCCCCCATCAGTCACTAAGAAAATGTC CTACAGGCTTCCCTACAGCCCTATTTTATGGAGGCATCCTTTCAATCAAGAATCTCTTCTCTCAGATGGC CTTACCTTGTTGACACAAGGTATCTTACTGCACAAGTGCCCAGATGATAGCAGCACCAGAACAGAAAGTC AGGAAGCCTCTTTTGAGAATTAAGCCAGGGGTATCTCCAATGTTAACTGTCCCCACTACTTGGCTTCTTA AGGATGCCACCTTGGAGATGAAATGTGGTCTAAATCTGGTTTCTCCATACGAAGAGAAGAAATTCAATCG GTGAAGACTCACTATGAGGATGACCTACATTCTGATATTCTTCCAGGAGAGAGGGACAACAAATTAAAAC AAAGCAGAAGCCACCATGCATGGACTGACTTCTCATTCCAGGGCTGGTACCCATGGGAAGGGAGTAGAAC ATCATTGGTAGTGAACTGAGTACGCTGTATCCAGAATTACCAACACAAAAGCCATCCCAGTCTGGAAGCA ACCATGTGACAGTGTGATAAGGATGAGAGTTAGTCAGGCAAAGAAGGACTGAGTTAACAGACACAAACCT CCTCTCTGGAAGATAAAGAGATTTCTGCCAAAACTCACAGGGAACTTGAAAGAGAGCTGAGGCGTTTGTA CTGGAGCAGCAGTTGCAAGATTGCCTAAAGGAAGCTGAACCCAGCCAGCATGTCAGAGAAAATGTATCCA TGTAGCTTGGGAGACAGACAGGGTCAGGAGAGCACCAGTGTGTCCTCACATAAAGCCCTCCATTCAGAAG ACAAGGGTATGCATATGTTCTAGGTTTGTGTGACATTAATGCAGAAGCAATCATTAATAAAATTACAAAA TGTTGTCAACACAATTATATAATATGTATTAATAAAGAGAATTTGTTCCCTCTACAATCTTTCTGTGCTC AAATTATATTTCTGGTACAATGGCTTTACTAACTATGACAATAGACAGAGCTTAATGTCTCATAATAAAA ATGTTAACAATGCCAGACTATAGAATATTAACATAGCCCGTAGTACCAGAAAATTTAAATGAAGAGATTA AATAAATAAGTTCATTAAGGGTTGATGGTAATAATAGTTCTACATGTCCATACAGATCTGAGTAAACTCC AGAGTGTTCTAAGAGTGTGTTCTATGCATTTTAAGAATAAGAGACAAATGAACACACTCACATTATCAAG CACTCCTAGAGAATTTAACAAAACGGACCCAGTGTTTCAAAAACTCCCTGTTTGATTAAATTCCACCCTT CGTTCCTCCAACAGAAGAAACAGAGCCCTCAAAAGCAGAAGACCATCTTGTTCTTTCATTTTTTTTCCTG ACTGAACACAATGCATCCTCTTTTAAAAGACACATATCCTGTGAGAACAGAGCAGGAGTAAGGTAAATCA TGGATGGAGAGATGGAAGTTCTCACGGTGAGCCACTGTCAAAGTTCCAGGCAGGCTTGCTCTCACTCTTC ATCCCTTTTGCATTGCCCCATAGATAAGCACAGGTATCTCAAGAGATAATAATACATACACCATGGCTTT TCTTTTTAAGCATCTGCTACAAGTGCCATACAAGCTGTGTTACAGGCTATGGATCAGTCACGTGCCTAGG ATATTTATGAGGCACATCTAATAATAGTCTCCATTTTACAAGTAAAAATAAGAGCACAGGAAGCTTGTGT GAGGTTGTAATAAAACCACTAAATGGCACGTCTGGGATCTAAATTCCACTCCATTCAATACTAAACACAT AGAGGTTTATGCAAATATAGCCACCACTATTTATAAGGAGAAAACACAATAGCCATGACTTCTGAAATGA GGCAATTGATAATGCTCAACAAACACCAGAGGTGCCACAAAGATTGAAGTGTCAACATCTATGATATGTT GGGGGAAATGTCAATAATAAATAAAAGCTACATAACCAACATCTTTCTTATGTATTGTTCTATACCCCAG CACTATGCAGGAGAAGTAGGTTATAGAGTTTTAGGAAAGAGTATAATAATGAACACACTGGCATTACATA GAGAAAACAAGTGTAGATTGGAAGACAGGTATGCAGTTAAAAAATTTCAGGTGTGGACTTAATCCTTAAA TAGAAATACTACATTACAAAAATGTCACTTTTTTTTCCAAATTTGTTCCCAAGAAATGAAATTGAAAGCA ACGTTCACATACAAGAGCTGATTAAGGAAGTGAAATCGATGCCGTGCTCTTAAGTTCATCTAGAAAATAA TCCATGGGTATAAGCACTATTTAAATAGTAAAAGAATAAGAGGAAGCAGACAGAGTGGAGCTTACAGTCC ACTAGTTTATGAAAAGAACATGGTTCACGAGTGCTGCAATACGTCTGTCTAGGAAACCTTCTAAATCTGC ATCTTGAAGCAGCAGCTTGGCCGAACCACTTAGCACAGCACATGAAAGAATTAACCCACTTTTTTCAGAG AAAGCATAGGGTCTTAGCTTATATCATGTGACAAACTGCAAACTGCAGATTTTCAAACAAAGCATCCAGA GGAAACATGTCTGATGTCTAGCTAGAGATACAGAGTTGATATTTTTAATTCAAAATGGGAAGAATAGCAG ATTTGATACAATTTCCTAAACACATGAAACTCAAGAAAAATGAAGACTGAAGTGTGGACACTATCCCCCT CTTTAGAAGTGGGAACAAAACACCCATGGAAGGAGTTACAGAGACAAAGTTTGGAGCTGAGATGAAAGGA TGGACCATGTAGAGACTGCCATATCCAGGGATCCACCCCATAATCAGCATCCAAACGCTGACACCATTGC ATACACTAGCAAGATCTTATCGAAAGGACCCAGATGTAGCTGTCTCTTGTGAGACTATGCCGGGGCCTAG CAAACACAGAAGTGGATGCTCACAGTCAGCTAATGGATGGATCACAGGGCTCCCAATGGAGGAGCTAGAG AAAGTACCCAAGGAGCTAAAGGGATCTGCAACCCTATAGGTAGAACAACATTATTAACTAACCAGTACCC CGGAGCTCTTGACTCTAGCTGCATATGTATCAAAAGATGGCCTAGTCCTCCATCACTGGAAAGAGAGGCC CATTGGACACGCAAACTTTATATGCCCCAGTACAGGGGAACACCAGGGCCAAAAAGTGGGAGTGGGTGGG TAGGGGAGTGGGGGTGGGTGGGTATGGGGGACTTTTGGTATAGCATTGGAAATGTAAATGAGCTAAATAC CTAATAAAAAATGGGAAAAAAAGATAATACAAATGTCAAAAGTTCTATAAATTAATACATAGTAAAATTA TAAGATTAGTGGAGTTTATAAAGTAAAATAAATATACTAACATATAGCTCCTTATTATAGGAAGTGATTA TAAAAAATTAAGAACTTTTTCATATCTTTCTATTTCTAATTATAAAAGAAATGGGGCAGATAGTTTCAAA GTATGAAAAGGCAAACATTAAATGAATATACTGTGGAGCTCTGTCTCTGTGGTATTCAGGAGAGATGTCA TCTTTATACTTTGCAAAGTTAACAAAAGCTAGGGCTTTGTGAGGCAATGGAATGGGCTCAGTGAGTAGAG TAACTTGTGACCTGCCAAGAGAACTCCCGTGGAGAAATGGTAGAGATGATTTCTGAAAATTGTTCTCTAT TCTCCACACTTACGCATCACTCGCATCCCAACTCTAATAATAAATTAAAAATGCAAATTTAAAAATTACT AGTCTGCATTCTCCTCCAGTTTTCTATACAGGCCGTGTGGGGATGTGACTGAAGATGCAGGCAAGGTGAG ATGATGATGATGATTCTCATTTCCAAACCTTGCACAGGAAAGGTAAAGGCAGGAAGATCAGAGTCCAGGG CCAGCCTCTCTAAAGAGGTAGCATATTAGAGCTATCCGGAGCATTTCAGCTCTGCACGGAAAGAGATGCA CTGAAACATGAAACACACTGAAATGATACTCATAAAAATCACCCTCCAAATCCTGCCTATCAACTGCATT CTACTCTATCCCGGGTCCTTAGGCAGTGCTTCCCACCAGTTCTTGCTCATCCTGAAAAAAAATCTCCCTG CAGAATACAAGCATGTTTAGGACATAGACTTTCAATCCATCATTTCTTCTATAAATATTAAATAACAAGT AGCATTTTGTGTGTCCTTGAATATCTATTAATGAGCAGAAAGGTCATTTATATTTCTTTTTATGCAAAAT GTCTATTCAAGTTCTTTTCCCACACTTGCACCCATCCTTCTCTATTCATATCCACAGTTTCCATCAGAGA AAAAAGAATTCCTACCTGTCCCCTCCAACCCCAGTTCACCAGTTTCATTTTCAATTTCTCCAGATTGTTG TTATTTTCACCCAAGGGGAGTTTTATACAATGAGTCAGGTTTATGTTTTTGAAGATGCCTGTTTTAGACG TTGAAACTGAGTAAGAGTTGGAATTTTCCGATGAGATGCTTTGGGGAATTCCTAAGTAACAAGTCACCAA CATGGAAGAGTTTTACTTCAGTCGGCTTGTTTTAGAGTGCTAGCTCACCCTCTTGTCGGCGTTGATAATT GAGCTGGTCCAACATCACCAGGACAGTTGAGCTTTGAGCTATGGACCTTTATCAGATGCCTCTAGCATTT TGTCATTAGTGACGCCATACTGTCTGGGCTACAGCTGTGTATATGTGCACACCAATGCAATGTTTATCTG TCTGTCTCTTACTCTGTCGAGTCAGTTATTGTGTATGAATTTGGGCATATACGTACCTGCTGTCTGTATG TGTGCCTATGAGTGTGTATATTTGGCATGCAGCTCCACATACAAGTGTGTGAGTGTGTACATGGAGGTCA GAGGTCAACATCTAGTGTTTCCTTTGATGGTTCTCCACCTTAAGTTTTGAGGAAGCATCTCTCTGAAACT GCAGCTCATTGTTTCATCAAGGTTGAGTAGTTAATGAGCTCTGAGGGTTCGCCTAGCTTTGACCTCAGCC CAGTGCTGGAGTTACCAATGTGAACTGCTGAATCCAGCTTTAAATAGGTGCTAGCTATCCAAAACGGATC CTCACATTTGTGGACCAAGATCCTGACCTACTGAGCCTTATTATTATTTTTTTAAACATGTGTAAAGACT ATTGAATATTTTTTAACTAAATTTATCCATCTGTGAACATTTGCCTATTATTTTTCTTTGGCTTCTAGGA TATGATGAATTACTTTTAGACAATAAGCTTTAATTTCTAAGATAAATCTTAATTGAACATAATATATTAT TCTTTCAGTGAACATCTAGGTTCTTTTTAATATACTAATGTGTTTCAAAATTATGTTCATAAAAGAATAA GCTAGGGTATCCACCACAATATTAATGTGAACCAACATTAACTCAAATAGCCTTATTTGGAAGGTGGTAT AGCATTTCATTGGCTTGAGAAATCCAGTTTATCATGGAAGGCAACACAATATAAAGTATGTGTTTTCAAA CTCACAGCTTTAAGGTAGAAATCAGAAGCAGTCAAATGCACTATCACCACGGACACTTCCTAAACATTTC TTTTAAAATCCCATGTAACAGAAAGTAGAAAATTTCAGCACCTGCGGTATCCCTCTAGGACTAATTCCTA ACATTGGCCCAGCTGATTCGTGTGGTACATGGCATTCAACAGGGGCATAGCTCATCAATAATGGCACAGC TCACGCCATGCCCAAGCCCTCCTAGAATCTTACCAAAATCAAGCATATGTTGTATCATCAAGAAAGCCAT TACAAAATTAAATTGACAGAAATCCTAGCTGTGAAAAAAGCAAGGAAAAATGATATAAGAACTTTTCAAT TGCTCGAAAATGAGGCAACACAGGTTTAACTAGTCTGTAGAACAGCGTAGAAGTCCCAAGAGACATCAGA AGTGCAATAAAGATATATCATGCTATCTTTAAGCATGGGAATATTTACATAAGTGAACTTCCAGGAAAGC TGGAGACTTTAAACTGAGAAAATAAGAGAAAACGGGAAAGTCAGTTCGGGGAAGGGCTGCAGAGTAAACA CTCCCAGTGCAGAGAGGCAAGCAAGTTGCACTGTAAGGAGTTACTCATAGACAAAGAAAGGGGAAGGAAG TTCTGCTAACTTCTGCCTCTGACTCATCACTGAGCAGCAGCACTTTAAGTTTCCCTGGGAAAAAGGAAAC AATTCAGACAGGTGCCTGCATCTGCCTGTTTGTATGGATGTGGTGACGTTCACTGGCACTTGGGTACCAG TTCTGTCAACTCCAGCTTCATTGTGAGACGGTGCCACCAAGAAACATGCCATATCTTTCCCATGAACAGC CTTCTTTGTTTCTTCCAACAAGTATTGCTGTTTAGATTAATTTCTCAGTCAGAGGGGCATGTTGCCTCTC AGAATATTCAGTTGATGGTGGTTCACTGTTACCAGTGGGTTTCCTCCATGCCCCTCTACTCCCTCTATCT CAGGGGAGGTTCCCCCACAAAGATTTTTGCTTGGGCCACAAAGGCTAAATTGTTTGGATTTCATAGTATC TTTTATATTCCTCGAGCTCTGCTATAGAATTATTTATGAGTACCCCCCCCCACCGCCAAGTCCCTGCCAC CAGGTCCAACCTCTTTATTCTCTGTATGGTTACCTGAGAAAACAGCTTATTTTAATATAAATTTAAGTAG TAGATTAATCAAAACAACTGTATTTCCTTACCTTTGCAAGTTTCCTAGTAACCATACAGAGAGACTAAGA TTTATTAATATTGCCTTGAGCCAGATACTTAGCAATCATTGCTCCACTCTTAACCTCCAAGCTCATCAGG TTTCCTCTCAGACATCAATCCCAGTTTATTTCCAGCCCTTTGTCCTTTTTGACTCAGCTCTCTTCTGATG TTTTTAATCAACCTCTCCCCAAGGTTCCCTCTGGTTCTTTCTCCACCCTTTCTAGAATCAGAAATCCCAC CTTGTTCTCTCTAAAGCTCTGCATTGGCTCGTTGGCTTTTATTGGCAAGACAGAAAATGAATGAGAAGCA ATGTTTAAACAAACTTGAGACAGGAGTTTCTAAGAATAAACATAGCAAAGTCATATCTAGATTGAAACAA GATATGAGAGCAGAGAAATCAATATTTGAATAAGACAGGGATAACCTTTATGCAGTGCACAAAAAAGTTA TGCCTACAGCTTCCAGAAAGATATTTATATTTGGCATCAATTGTTGCCATCGTCGGTATGGAAACAGAAG AGTTCCTGGAATGTCAGCATTGGTGAAGTAGCAATGTTAGGAAGATTCACAGAGATGATCAAACCAGTGT TCACATATTTTCTATAATATATGCATAAGTTCTGATGTCTATGATGGCTACTCTGGCTTGGCTGAATTGA GAAGATGCCCAGAAAACTGGCAATCCATACTTCAAGGGGTATCTATGGAGGGGAATTTGGGAAAAACTGC TTCCTGGGTGAATGAGCAGGGCAGACAGGGATCCTGAGAGGTGGGAGTGGCCATGAGCAAAATGGACCAC AAAAGTAGACGTGGGAAGCCAGCTTTCACTCACACTCTCAGTCCCTGGCCAAGTTCACTTATTTGTTGCT GTTGCCTGTGGACATAAGACTCCAGCCTCTTTTAATTATTGATCTTAGAATTGCACCAGAGGCTCTGTAG GGACTGCCAGGCCTCTGGTCTTAGACCAGGGCTGTCCCTTGGCTCTTCTATCATTCTGAGATTTCTAGCT TTTCCAGTTAATGCAGATTTAGCTCTCCACCCTGCAAGCAGTCACTGCAGGATTTCTCAGCCTCTGTGAT GTTCTGAGTCAGCTCAATAGGTCCTCATTTGTAATAGCATGTACTTTTGGTTCTGTTTCTCTGGAGAAGC CTGAGTAATATGCCCAAACTCCAAAAATTTTTTCTAACAAAACTGTTAGAAATATGCCAGAGTCATACAC AAAAGAAATGTATTCTATTGAGTATTTCCAATATCTACCCAAATTTAAAAAAAAATCAATGATGGCATTT AATATATTTTAAACTGCATTACTATGTGCAAGGTGTTAATATTTACTTCTGTAATTCCATACAGAAAAAT GCCATTATATGGTGTTAAGTACACAAAGCTACAAAATAAAATTTTCGTTCTTTTTTAATAGAGAGTATAG AAAACTAAGAATAGAGTGTTGAATTAGCTGTTAGTTACATAACTGGCAATATGTCTTCCACTTCACACTT CTCTCTTGGCTTCACATATACTATAGTGAACACACATTATTTTGATAATCAGAAGGATAATAAGAAATAT AATTGAAAACTTTAGCCAAATAGAGTTTAACATGGATATCAGGACAACCAGAGCCCATAGGCTGTGGTAC TCGCTGAAATACAAAAGGAGAAGACTTAATATGAAAACACATACAGGAGAGACCTCTGAAGTAGAAATTT CTGGTTCCTTCGGAAATTCAGTTATACCATATGATGTTGGCCTGTGGCAGACAGGTGAAAGGGTGTTTTG ACGAAGCAGACAAGTGAGGGGATGTTTTGCTGAAAGAGACACATGAGAAAGGGCACATGATGTTTAGGAA GAATATAAATATGACTCCACAGCCAGTGGGAGGAGGCTCTGGCATTGGTTCAATTTGCTCTGTCTTGCTG GTCTTCACTGATGGTGCTCTTGTATTGGTTGGCCTTGCATCATGTTGCTGATCTTTGCTTGTGGTGACTT CATAGAGAGTAACTCACCAAAGAACTTCTCATGATATTCTGCCAGCTTCTTGTTGCTTTAATGGACCTGG GGCATTCGCCAGAGCCTTGAGATTTCTTCTGGATTGAGCTGCTGTTTCTGATTCACGTTTGTGTTTGCTA TTAGACTGAACTGCTGAACTGCTGATACCCTGACAATAAAGACTGGAATTGTCCAAAGAACTATTTCTAA ACAGGTCTACAACCTCTGTTTCCTATTAACCTTCCTTTCCCACTACCTCTGGTGGGTGGTGAGTTAGAAC GGAGGTTGAAGCACTTAAGAACCCTTATTAGAGTAGGTTTTGAAAATTCTAAGCCTACAGAACCTGTATT TACATACAACATAAACAATTTTTTTTCATTGTACAAATGTGTATTTTTACGATGTAAGTATTAAAGGGCA TGAAGAATGCAACAGGTGAGATGGGCCAACAAGACTGTCACTGCTGCCCAGGGTTCTTGACTGTAGGGCC TATGAACGTTCTCAATTGAGTGTCCAAAAGGAAAGCTGCTTTGATGGCAAACAATCCTATTCAAAGTGTG CTTCCTTGGGTCCTTATGGTAGTAAAGAGCAAGGGTCATGAAGTGACAAATTTTACTTAATTGCCTGTGT GTTAGATGCAAATTAAAAGAGATTCCTTAAGTTTCTAATTCAACTCAGCCTAAATTTCCAGCCTTCCCTC ACATCCATTGGAAAAGCTATCACTGGTCATCCTCCTATAGCCAGTTGAATGACCTAGTTTCTGGCTTTTG AACAAAGGAAGCCAGAACGAAATTATTTAGGGAATCCAGAGAGTTGAAAAAATTCATAAAGCATGTGACA GTTTTCTTATGTCTTCAGGAGCCTTATTTGTTACTGGTTACTGCTGGGTAGGGCATTTCCTGCTTAAGTT TCTTACATCCTTCAAGTGCAGTTTGAATCAAAGAAAACTCTGGCACAATAGTTTATTAGTCCTATAGTTT ATCACGTGGAAATTTTAAAAATATGATGGTTATTTATTTTTTATCACTATCTCACAGTGGCAGAGTAAAT TGGAGCTACAGTGTTTTTGCTAAAATTGTCCCAACTTCTATAACTTCAACATCCAGGATACTGCCTGTCT CTTCCTTGAGCCCCATGGCAACAATTCCTAAATGTGTGTGTCTAAAACAATAAACACGAATTGATTTTTG AGTTCAGGGAATTTGCCACAAAACTAGCTTCCCTTCGAGAAAGCCTGGGAGATCACTGAGTAAATGTCTG CTTAGTTGATCAGTACCCAGGACATGTTCTTTGCACTTTTTCTTTCAATTTTTATTATCTTTATAATTTT CCAACAACAAAAAAATGACTGAAAAAGACAGGAATTAACTTTCTGTTTTGGATTCTTGTAAAGTTATTCT TCTAATAAGCCTGGTCACTTCTATCACCTCCCTTACCTTGTCTACTGTCAATCTTGGTCTGACCACACAC GACAGAATTTTGTCTAGAGGTCACACAGAAATTACAACAGAGAATAACAGTGAAAGATATGCATTTACCA ATAAGATGCCAGAGCTTAGTTTTCTGGGTGTTTGTTTGCTTGGTTGATTGGTTTGGTTTTGCTTGGCTTT GCTTTGCTTTGCTTCTTTTTATAGCTCCCTACACTCAATGTATCCAGACAGTGTTCTCATATAGTTATCA AATGTTGAAATTATTGATCAAACAATCAGACCTGTGCTATTTTCTAATCTGTTTGCATTAAAAAAAATTC ACTGAAGAAAGGGATCTTAGAATTACTTTTGAGTGAAGCATGAAGTATCCCATTCTTTCATATTACCCAG GAACAGAGCAGAAAGTGAGGGACAGAATTTATTCAACATAGGTGCCAGAACCATCTGGTTTGGAAATGAA ACTCAGCTCTGGACACTCATATTCATCAATGACTAAGGTTCCTATCAACCCACGTGATAGAGGATAACTA GTCTGGACAGAGAACTTCTAAATAAGATGACCTAAAATATTCTGATCTGACTCCAGGTAAAAGGAAACAA CCACCTATGTTATGTGCCATACTCTCTGACCTCTCAAATGCTTGTAAAGGCTGGCATTTCCTTCTCCAAT AACATGCTCGTTTTCTTAAACCTTGAGTGTAAACAACAGCACCTTGCTTTTCATGGCCTATTTATTGTTT GTCACATTATATTGCATTATTATTACATTGTTGCATGATACAATTCTTAAAGTTTGCTTACTTGGCCTCC TGTAGGCTGGTTATCTGCTGACATGGGTTTCTGTGTTCTTAAGGACCTCACCAGCAAGGACCATGAAGAG GCAGTGCTTTGAAGAGCCAGAGGGCCATGTGTCAAGGTGGATTCTGTAGGAGTAAAAGGCAAGGCTTAAA GAGATAAAATTCATACTGACTCTTTTCCTCAAAGTTATGGTTAAATATGGACCTGTCATTCTGATAAAGA CTTGTGTGGAGAAAACTTGAAAAACTAGATCTGAATTGTTTTTTTTTTTTTAAAACATTCCTAAAGTCGT CAGTTCCCCACAATATAATTGACACCCATGTTGACTGTAACTCTCATAATAAATCAAAGCAAATAGGACA TCAAAGATAACTGTGGGCTTCATTACAATTACAAAATCTAAGCAAGATAATGGAAGTTACTTGTAGACAT CTGTTCTAAGATTTTCCACTCCTGTACTTAGAAATGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG TGTGTGTGTGTGTGTGTGTGTGTGTTTTATCATTTACAAAGTATATCTGCTTTTTAAGGGGTCTAGTTAC ACTGTTAGAGAAGCTTAAAATAGCCTACCTCCTATTTTGCTTTCCAGAAGTACACACTTTATGCTTTTCA AGAGTAAAATAATATATTCTATCTTTTAAAATAATTAAGAAACATGCACATTACAGAATGATTAATGATA TGAAGGATATCATATTTATAGCTTTAATATTATGTTAGATAAGTTCTTCTGATCGTTATTGGGATATTAG GAGTTTGAATAAAAAAGAAGCAGCTGCATTGTGTTGCCTAAACTTCCCAAGTGAGTCCCAAGTGAGGGTT TACATATTAGCTCTAAGACTAACTCACAGAGAGGAGAACTCTAACTTAGCATTTGATATCAGCAGCCCAC CCCAAGACTTCATCTCTAGTCCACATAAGGAAGAGTGAATGCCAGGGAGGAGAGGAAAAGGCAAAAACTG GAGGTACAATTCAACAACATCCCTCCTAGAGGATTAGCGCTCAACAGGATGCTAGCTTCTCCTTGTCCTT CTCTTTCCCATCCTGTCATCCTGTCATCTTCTTCTTTCCTTTCTCCTCTACGATGTGTAAGAACATGGCA AAATGATAGCCATCTCTGGAGCAGGAAAAATCATCTCACCAGAACACAAGTCAGCTGGATTCTTATTCCT AAAATCCACTGCCTTCAGAATTGTAAAAAACAAACTCTCCCTGTTTAAACCACTCAGTCTCTAGAACTCT GTTACGATACCCACAGCTGACTCATACAAATTATAACACATCCCCACCAGCCAGGAAACACCAGCCAGGA AAATTGATTTTCCCTTAAATATGCCCGTAGAAAGGAATAAAATAACAGAAAAAAATTATTGAAGGCTTTG CTTCAATAAAAATAAATAAAAATTTCCACTCTGGAAATCAGTCTGGCGGTTCCTCAGAAAATTGGACATG GTACTACCAGAGGATCCCGCAATACCTCTCCTGGGCATATATCCAGAAGATGTTCCAACTAGTAAGAAAG AAACATCCTCCACTATGTTCATAGCAGCCTTATTTATAATAGCCAGAAGCTGGAAAGAACCCAGATGCCC CTCAACAGAGGAATGGATACAGAAAATGTGGTACATTTACACAATGGAATACTACTCAGCTATTAAAAAA TGAATTTATGAAATTCCTAGGCAAATGGATGGACCTGGAGGGTATCATCCTGAGTGAAGTAACCCAATCA CAAAGGAACTCGCACAATATGTACTCACTGATAAGTGGATATTAGCCCAGAAACTTAGAATACCCAAGAT ATAAGATACAACTTGCCAAACGCATGAAATTCAAGAAGAACTAAGACCAAAGTGTGGACACTCTACCCTT TCTTAGAAATGGGAACAAAACACCCATAGAAGGAGTTACGGAGACAAAATTTGGAGCTGTGACGAAAGGA TGGACCATCTAGTGATTGCCATATGCAGGGATCCATCCCATAATCAGCTTCCAAATGCTGACACCATTGC ATACACTAGCAAGATTTTGCTGAAAGGACCCAGATATAGCTCTCTATTGTGAGACTATGCCGGGGCCTAG CAAACACAGAAGTGGATGATCACAGTCAGCTATTGGATGGGTCACACGGCCCCTTATGGAGGAGCTAGAG AAATTATCCAAGGAGCTAAAGGGAACTGCAACCCTATAGGTGGAACAACAATATGAACTAACCAGTACCC AGGAGCTCTTGTCTTTAGCTGCATATGTATCAAAAGATGGCCTAGTCGGTCATCACTGCAAAGAGAGGCC CATTGGACTTGCAAACTTTATATGCCCCAGTACAGAGGAATGCCAGGGCCAAAAAGGGGGAGTGGGTGGG TAGGGGATTGGGGGGGTGGGTATGGGGGACCTTTGGGATAGCATTGAAAATGTAAACGAGGAAAATACCT AATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAGAAATATTCTCTAATAAGAAAA AAAAATTATTGAAGGCTTTGCTTCTCAACAAAATCCTGTGTGTTCTCACTAATATCCTAGAGTGGCTACT ATTGCTGTGATAGATTCCATATGCAAGAGCAACTTGGATTGGAAAGGGTTTAATTCAGCCCTCAGTCTAT AATACATTATGAAGAAAATTTAGGGCAGGAACTAAAGAGAGGCTATGAAGGAAAACTGTTTACTGCCCTG TTCTCTTTATCAGCTGACTTTTTTATATCACTAAGACTAGCTGCCCAGGGATGGCACCACCCACAGAGGG TCATCTTCTTGATTAATGATTGATATGGGAGGTCTCAGCCCCTGTTTATTATTTTTTTCTCAACAGGGGT TCTCTCTTCCCAGTTGATCCTGGCTTCTGTCGAGTTGACAAAAAAAAAAAAAAGTCTAACAAGACAAAGG AAACTAACATACTCAGATCTGATGAGCCATAAGATGAAGAAACGGAATTGAAAGTGAGAAATTCAGCCTG CCACCCTCTATAGGCCAGTGCAGAAGCATTGTTAAAGAGACCTCACACCTATTCTGGAGGAGCAGCCCTA ACCAAGGCCCCCCCATCCAAAAGTCTTCTTCAATCCTTAGCTGGTCCAGGAGAAATTCAGCCTCTTAGCC CTCTACCATCTCCTCATGTCCAGCTTGAGGCAGTTAGAATCAGAAGGAAGCTAGAAAGCATGCTACATGG ACTGTAATGTTCCTGCTTTTAATTAGGAAATATGGGCATAAGACTCTCAGTATAAGACTAGATAATTAAT ATAGTCACAAAGTTGCCCCTATGTAAAAATTAGGGAAAGTAATAAAATTTAAAATGGGAGGGGGGAATAG ACAACGTGCCCCTCCCAAATAAAAGGCATAGGAAACACCTTGTAGCTTTTGAAGTTTAAAATTGCAATTT GAATGTATCTATGGAGTCAAGCATTAAGTTTGAGAAAATAACAGCAAATGTTAAACGTATTAGACAGGGG AGGTTTAGTTGCTTTCATTAATCAAGCAACTATGAAATATATCTATGTTATCTTGTATAAAGGTATTTAG GCTAAGTGGTGCTTGAGAAGACTATGACCATGTCACTTAGCACTCTGAAAACACCATGTCTCATTCAAAG GAGATGAGAAATATGTTTAACTGAATTACCACCCCTCTTAATGCAGTAACCAAGTTAAGACCCTTTGCTT TGTTTTTGTTAGGAAAAGAAAAACAAAAAACAACAACAACAAAAACTCTAGAAATGGTGAACAACCATTA CTTTCTATGAATGTATTTTCTGTGTCTGGTTTTCCTGTTCCTAACTTAGTGATCCACACCTTAAAAACAT GCAGAGCTGTGCTGTCAAGCTGTGCACAGTAGCCACAAACCCTACGTGACCATCTGAGGGTAAATGAAGT AAAAGAGAACAAACCAGAAATCCACTTCCTCACAGGAGCCAACCACAACTCCAAAGCTGAAAAGCTGAGG CACATGCAATTTTGAAACATGACTTAGCCACCAGCCAGCAATACCACAGCATGCGTGAGCTAACTCAAGA TACTTTCACCCGTGGCATTGTGAAAGCAGTAATATCTACCTCACAGGATGATTGTGCAATTCAGCATCTC AGGCATGTGAGTTGTGTAAAATGGGCCATGGCACACAGAGAAGACTCAAAACTATTGTTGTATTTGTATT ATAATTTTATGTTCATATTTGATTCTTTTACTGCTACTGGTGATTGGGTGACTGAGACTTAAACTGTTTA TCAGATCTAAGAACATGAGCCAATACATTTGTACCTGAGATTATGATAGTCTCCTTGAGATGACACACCT CTGGACAGGGTCTTAAAATTTCGAAGAAAGTGGTCAAAGATCATGAGGAGTTGGACAAAGCCACAAAGCA GAACAGGAGGGTGAGGTTTTCAAAGGTTGAGAGCTTGAGATAAATTTCAAATAACATAGGTACTGCAGAG GTAGAAGAATGAGAGTGGAGAAGATGTTAGAGGGATCAGGCCACGATGTTGGAAAGTGAAGCAAGAGGCA GGGAAGAAGCTCTGTAGTCTTTGCTCCTCAAAAAATATATTTCCTTTCCATCCATAGGCCAATCCATATC TTGTTTATTTGTAGGTTACAGCACCAAAATAGTCTAGCTAGGCTTGACTTTAATAGTGGTCACTTTTGTG TTCTTTCATCCCTTCTCCATGTTATCTTTATATGAAAGCATTGTGCATATATGCTACTTTTAATTGAAGA AATAGATATGGAAAGCTGTTTCCTGAACTTTGGAGATTGGCTGTTAAAAATAAACTACCTCCCTCCAGTC CAGAGATATGTCATAAACACAAAAGATCCCCAGGTATGCACTTAAAATAAAGGATCTGCTTTTGATTCCT TGTGGCATTTAGATAAAAAGATCCATATAAAATGTCAGTTACAATTCATTATAAAAATCAGCAAATCATC ACAAAACAATTTTCCAGTGCTGCCACTAGAGATAATTGTTTAACTATTAAAGTGTATGCTATGATTTATA GCAGTCACTCTGATTCCAATCACAGCGTGATCACTCAGCACTGCCACAGAAGACACACCCCATCTGTTTG TCTCGGGAAATGTTGCATGACGACTGTAATGACGCGTACCTGCTATCACCTGCTGATAACATCCCGTCAG AAGGAACAACAGCCTAATGACACAGCTCAGCTCCTTCTGGCAAATGGCCAGATTCAAATATTTCTACTGC CTCCCGCAGAAGTGAGCAGCATGATTACCACAGTCCTCGGTAATAACGCCATCAAATAAAGCAATCTAGA ACAAAGTGGTTATTTTGTAGAATACTAGTATACTTTTGACCACAAAAATCCCTTGGGGTCTATTCTGTCT ACAGACAGTCTCACCAGCAGTGACTGCCATTCTCCTTTGCTTTAATTATTCTTGCTGATGAGACCTCAGA CATCACTTATTTTGAATACTTGGCCTTTTGTGCAACATAGAGAGTGGTTTACACTGTATATGTCAATCAA CAGAGTAGTAACAGATTTCTGGAGTGATCCCAAGCATTATTTGTCTACAGCATTGCTTGAAGGGCCCTTC CTGACAAGGGAACAAAGCTTCTAGCAGCAATCCAACAGCTCGCTGAAATGCTAAGATCTTGATTAACCAA GTTTATTTGTCAGTTGGCATAACAAACGTCAGATGCCTGGGCAAAGAAAGTGGCTACTGCTTTCCCTCTC CCTGGCCTAGTCAGCAGACAGTATCCTCACTCTCAGTTATCATTTTTTCTTCTATAGGGGTTATGTGCAG TTATTTCAGGTAGAGCCAGTATATCCCTGGCCTCCAAGACAAAGGACTTAATTTAAAAATATATCTTCCT TCCATTGACCTAATCAACTACTACTTGTTAAAATAGTTATCAGAAGGCCAACTATTACTCTGGTAAAGTA GTATTCATCAAGCTACTACTAAGGATGGTCATTTTTACCTTGTTAATTAAAATTTTTTTCTCTGATTTAT ATTACTTCAATCATTCTCTTTAGATTCAGTATCAAGAAAGTGGGTTTCCTAAGCACCAGCAAATGCTGTG CCATTCCTTGTGGGATGAAGCTTGCAATACTCCAAGAATTGACTTGTTGAGGGAGGAACTGAAGGATCTG GCAGACAGACATCAAAAACTTGCTTCTTACATCAGGACAATCTCTCCACATGGAAGGCTGCCATGCTACA TGACTAAATTACAGAAAAGTCGATTCTTTAACACACACACACATGTTGAGAACAAAGACACCTTAAAAGG CTTTGCTATAACTTAGGACTTAACATATCCTAAAATGTTAAGTCATATAATTCAGGTGTGGTCCCCTGTG CAAATGGTGTTTGGAGGCAGAGTTGTGGGGAAGTACTTGCATCTTGGAAGTCCTGGCTTCAGAAATAGAT TATTCTATTGGTATATCAATATTTGAATAGGTTGTACAGAGGAGGTAGAGCTGGGTCCTAGTTAAGGGAA GTAAGTGACTGAGGAAACACCTTGGAAAGATAAATTGACCCCTTCCTGTTATATTATGCTATCTGCCCCC TAGCACCTTGAATTGTACCTTTGCTCTCTGTCTTATTTCTGCCTCACTACAGGCTGAGAGACACTGCAGC CATCCAATCATGGACTTTAAACTAAACTGTGAATCAAAGAAAATTATTCCTACTTCCCCCTCTGCATTGT TTGTCATATTGACACCAACAATAACAAGTACAACACAGTTTTATAGAATTCATATGCCATGTAAAAGCAT AACATAAATCATCCCCATGTAAGCACGGGCCATAGCCTTCTAGGCAAGTAGCATCCAGTATACCCCTAAC ATTTGCTTACAAGAATTTGTTTCTAAAAAGGCAGAATCTCTGTCTGTGGATTGTTCTCTGAACAATGATA GAGGATTCACCATCTTCTATTATATTCTCATGAAGGCTACCATGTTCACAATTAAGTTACTAAAATAATG GTAGCTTTATCACATTGAAGCTGAAGAATTTTTTATAAAATCTGGATCCTCATCAACATTGTCAGAACTA TGAAGGAGATGGGAAGCCAAGAAACATGAATATACATTTTAGTTCTGTCTTCAAGTAGCTTCATGGCCTT AGACAACTTATCTTTGAAATACAGAAATTATATTGGAGGTGACCCTAATTCAGTGTTGAAGTACCATACA TAAAGTCATAGTTGACCACCACAGCATATATTGCCAAGTCTTTTCTTTCTTCAGAATATCAGATGTAGCA GCCCAAGAATTTCATCTAATCATCTTCTTTTTTTTTTTCAGAAAAGAAAACACACAGAAATTAAGTGCAT TGTATAGATTTACACAGTGAGATAAAGTTTGGGTTCAACAATGTGTTTTGTCCCCCCTGCCCCGGGGCCT TTGCTGTTCCCCAGTTGGTTCTTGATCTGTCAGTAAAGAAGCCTCGAGACAATTGCTGGGGGCTTGGGGG TCGGGGGAGGAGGAATAGACAGGACGTCCAGGTCCCTAGAGGAAAAGAGACAGGTACAACTCATCATGCT TTGGAGAGAGAAAAGCTAAACAGCCATGTGAGGTCTCAGGAGGAATGGCCACTCACACAGGTGGGTGGTC AGGGATGTTTACTAGGGACTAGATGTATCTGAGCCACTAAAGTTTAGGGCAGGTGGGAGGTGCAGAGATA AGACTATTAATAAGGGCACACTTTTCCAGGTGGAAGATAATAGTACCCAGCAATTGTGCCAAAAGGCAAG TTGAAAATGAGCAGCGTATATGTATTTTATCCATGGATTCAAGGAAAACTGGGTGGGGTCTGGTAACACA GTCCATTCCCAGACCTTAGGCAAGGTAGGGAAAGCTACATGCAACACCCTCTCCCCAACACACATTAGTT ATCTACTAGAGTTTACTTAGCTACCAAGAATATGTGAGCATTATGCTAGCTGCCCACACAAACTTTAGTT CCGAGATGAAGGCAACTTGTTCCAATTAAGTAGGATTGTGATGGTTAGTCTTGCAACTCTACATGAATTG ACTATATATAAAGGTGTACTTATTAGTAGATTTTTGAATTGCTGTTTTACAGCTTATAATTAGCCATCCT ACAGCATCAATACTATGATTCAAAATGCTATCACACTGCTATGAATTCAATCTACTGTTTGCAAATAGAG AGCTTAAGGGTCAGAACCAACCAGTGTTCCAGAGTAATTCTCCTTATTCTAACCCACAATAGCTCAGTTA TCTAACTTCCTCCTTGACACTTTTGCTAACAGCACTCTCCTTTTTGCACTATCTGGCAGACCATAAGTAT CCTGTCTAAACATGCTCTTATCGTTGTCTTCAGCACTTCCTTTAAGGTTGAAACAATGTTCTATCCTCAT TAGCTTCCAAGAATATTTCTTCACATTTCTGTATTAAATTCAATATTTACTATACCTTTTTAAGCATACA GTTCTCCAATTTTGAAGGGTCTCATTTATCTTTACTCCCTTTGGAGCTTGACATTAGCTTTTTTTTTTTT TCCCTCATTACTAAGAATGTACTGACAGGTTTTTTTGTTGTTGTTGTTGTTGTTCTGTGTTATCTATTCA GTCTTCAAACCACAATTTGTCATGGAGAATTGTTGTGTCTCTGCTGGGAGATGTTGTATGATCTGTACTA ATGTATTATGCTAGCTAGTGATAATTTACTGTTGAAAAATATCATAAACTTACTCTCTAGTAAACTTGCT GAACTCTCTAGTAAGTTTTAGTAATTTCTTGTTTTACATAGCTTTCATATATAAATTATGGAAGTTTACA TTCTCCTAGTCACATTTTTATAAATGTTGATTATTACCTGTGAGAACTACTAGCAAATAATAGTCTTCGT ATGAAGAATGCAGAATCTAAAGTGAGACCTGAGTTCAAACCCATTCTATCACCTTCTAAATGACAGCACT GAACAGACTATTTGAGTCATATGTGTCAGGGATAAAAACCAGACACACAGGAGAAGAAGATGTTGGATGA ACCAGTACATTCCTAACAAGGGCAGCATGTCCTGATGCCTGTCTGTGAAGGAAAACAATGCATTTAAAGT CAGTCATTCTACACCAAGGGTACTCAACTCTAGATTGACAGCTACTAACTGCCCCATTAGATAATACATC TCTGGAGAGTTTCATGATTACTTATGTCTGTCTCTCACATAACTTGGCAGAGAACCATACACAGAGAGAG TGATCAGTAAATAGGCACTATTGTTCAGCAGAACTGCCCTGATGTTAGCCTTCCAGAACTAAAATCCACA GCTTGGAGGACCTAGTTGGTAAATAACAGTGGCTGGGGTTAAGAAAGGCCAAGGTTAGTAAGATAAGTAT ATTCATGCCAAGTCAAACAAGACCTCAGCAAAATTATGTCTTATGTTTCTTCACAAGCAACCACTAGGGC ATTGCTTTACAAGAGATAACATCTAAAGCTATAAACTATAGATTTGTAAATCATCATTGGACTCACACTG TGACATTATAATTCTAAGATGATATATTTAATCTTTAATATTCTTGTGAGAAGAGGCAGTAGATAAATTG CATGTCAACAGCAAGCCTAGTTTTGTAAAGTTCCCTTATCCTCTTCTGTTGAATTGTTTCTAAAATACTT GGCAATGAGAATTAAGAAAACCAAAAGAGCCCTGTTCGAATGAATTTAGAGATTAGGGTTTGCCGTATTT CAATTTCCTTTCATTTATGAAAGCATTTTCTTTGTAATTGCAAATCTGCCCACTCTATAATAAAATGTGG CATAAAATATTAGACAGCCCTGGGTTGGTTTTATTATGTATTCTATATTCTAAAACATTCACATTTCTAA TAATGAATCCATGTATTCTGCCTGCATCCTGGGTCAAAGTATGCTCCTAGGGGATTTTAGTTCACTTCTT CATCCTAAAAATAAGGAATACACTAAGATTTCTCAAAAGCCATATGATCTCACCAGGGATAATTCATACT GGTCAAAACTAAATACACTTCCTCAGACACTTAGTTTTCTTTACACTCAACAAGGAGTGGCTTAGAGGCA GCACAAATAACATTTGTAAAGCTCCATAATCTTACAGGCAAGGTGGCAGGCATTCAAGGATTCACTGCTT CCCTCATGTCAGCCCTTCTGACTCCATTGCTGCAGCTCTGAGTATTCTCCATGCCTGAGAAGCTTCATAA ATACGTTTCCATTAAACTGAGCATTACTGAAGCTCTATTCTATATGTATAGTTCCGAATCAAACAATTCA ACAAATAAATAATATACACTAATAAATCAATTATCCTTGAAATTGTTCTTCTTTCTTTTCCCAATTGTTT TTATTCTTGGGATGCATGTGTCTAGTTGGTTGGCTGGTTGGTTACTTGGTTGGGTGGTTGGTTGGGTGGT TGACTGGATGGATGGCTTTTGGACACAAGGTCTTACCAAGGTAGCCTAAGCTGACCTTGAACTCAGCATC CTTCTTCCTCCACCTACCAGTACTGAAATTGCGTGGTGAGTTCATAAAGGTAGAATGCTGACAAATTTCT AGGAGCAAGACAGGAGACCGGTATAAAGTTTGATTTGGCGTAGAAACATTTATACAAGAAGTGACTTTTT AAAGACATACATGTGAAGATAGGCACTGGGTAGACAGAAAAGTGTTTCAGAAGACAGCAAGCAGTGCAAG TTTGACATGCAAGCAAAAATATGTGACGCTTGACCTTTTTACTTGAAGATCTCAGAAAAGAGCAAGGCTT TCCCAAAGTCTTCACTGAGCATTGCTTCTCCCAGGGTAACCAGCTCTGTGACAATCCATTGTGTATATGA CCTGACTCCTGAATAATAGTCTCAGAATTGGCCATAACTGATAATTTTTTACTTGCTAATTTACAGGCTA TGCTCTTAGTCATTTGATCAATAGGCTAGTACTCCAAATGACTAATATTTTCAATTGTGTAATCCACTTT TATTACAGTCTTGAGATTCCAAAATTAGTATCTCCTTTTCTTATTTTAGGTGTAACCAAGATCTATCTAT CCTTGGCACCTTTAATTTTCCTATCTCTCAGGATTAATTCCCAACTCTTGTCTCATGCCTCCTGGTGACT CAAATCTTGTCAAGAGTTAGAATACAGACCATATAGTCTAGGAATTCAACATTACTGAACTGGCAACTGA AGCAAACAATTTAATGGATAACTAAGATGTTAATAAGTAGATTCTCTTTGAAAATACCTCTTTTTTTCTT TCTCTCACTAGTTTATATTGTTCAGTTGGTTGATTGGTTGTTTGGATGGGTGGTTGTTAGATGGTTTTCA AAAACAAAAACTTATGCTGTAGCCCAGACTGGCCTTGACCTCAACATCTTCTTGTCTCTGCCTGTTGGGG ACTGGAATTGCCAGGTATGGGCAACCATGCCAAGTCTTTACTATTTTTTTAGCCTGTTGGTATTCCTTAG TAGCAATGTATCATTAATTCGTTCATTCATTCATTGAATTTCTAATAGAAACTACCATATGCCAGAACCA ACAAATGACAGAGCTGTGTCAATGAGGAAAATAATAAAAATATATGTTCCACTATAGTTTACATTGTAAC AGAAGAAAAGAATTGTTCACAAGTTATTAAATCAAGCATATACACATCCAGAGTTCCTTTTTATGAACAT AAGAAAAGCTTTGAAAACTGAAACTTTCTCTGTATCAGTTAATTCAACATGAATTGAGCAGATATATGTC TCTATAAACTGCATTTAGTTCATTTTTTAATTGGTATCTGAATGTCTTATTGCTGCCATTTGCACCATCA GATATTGTTTAACTTTTAAAACCTGGAAGCCTGTATTTCTAATCATATTTGATCCTCTAAGTTTCAGACC AAGTATTACAAAGTTGCATTGTATTAGGTAACAGCAGAGATGTAGAGAAGTTGTGAGGTAATGGCTGTTG TTGGTCATGCAATTTGAAGACTGGTGATAACAGAAAACTTCAATGTAAATGGGGTACTCAAGTCAAGCCC CAAAGGAGGAGGTAAAGGAGAATGGACATATCTCAGATAAGAGAAGTCTAGGCAAAAATAAAATAACAGA AACTTCAAAGGTTCTTGGGCAAGAATGTAGAATGATGTTTAATAAGCAGAAACACTGTATTAGCAAATCA GTGAACCAAGAAAAGGGAATGAAGAGATACTAAGCATACCAGTCATTATAACTGAATTTGGATTTTACTT AGAGGAGAAACCAGAGGAAGATTCTGAAAAGAACAGTAGTGTGTCCTAAGTTTTTGTTTGTTTGTTTGAT TGTTTGTTTGTTTGTTTGTTTTTTACAATACCACCCTTTGTAAAATATAACTTCCAGAAATAGCTAACAC AAAGCAGTGGCTTCCAGCCTGTGACTATCTATGCCAGGCTACTTGTCAAAAAATGATGTCTATGTCCTCT GATCTTGAGTGGAGGATGGCCTTATGAGTTCTTTTTCCCAGAAGAAGGAAACAAGTATGGCAGTCAGAAC TGAATGTTTTGTCCTTAAGTGTTGGCAGGTTCTACTTCCTGTCTTACAGGAGTTCAAGATTATATAAAGT GGACATGGGCTCCAACCCTTAACCCAGAAGATTTCTGCAATTGATTCCCACTAACAAAAAGACCATTAGC AGGTATATTTACCACACATAACAGGAGGCATCATGCCAAGCAGTAGATGGCCAACACAAAATGAACTCAG TGGAATTTTTGTAGACTCATATTACATTCTTTGGGCATTTTTTCATCTTAGTTGGCTTTTGTTTGTATAC TATGGTTTCTGATGTTGTGTTTTTATGGGTCCTGTGTGTGTCTGTGTTTCTTGTGGTTTTATTTTTAAGA TTTATGGTGTTTTTGTTGGTTTGCCTGTCTGTTTGCTTTGTACGGCAAGAAATAATAGGCTTGGTGTTGG TTGGGAATGGAGGTGGGGCGGATCTGGGAGGATTCAGGGGAGGGGAAACCAGGATATATCCTCAAACCCC TGGGTGTAAGGAAGTTACACCCAACCAGAGGGAAAGTCTTGTGAAAGACAACTAAGTCACACAAGCAACA GGATAAGAAACGGATATAAAGTGCCATAATCCTGTGTCCATCATTGTCTTGGGCCATAATGAACACACCC CTGCTCAAAGGGTGCTGGCCTTGATAGATAGTACTGAAGGAACAAAATGGACTTTTGAAAGTGACAATTG TCTGCATTTAGGTGCTAAGATTAAGTATAAGACCAGAAAAAGAAGAGAATTTCAAGCTGACTGCAATGGA CCATACTTGTAAATACAGCACAGGAGAGGCAGAAGAGGTGAATGACAAATCAAGACCACACTATATAGCA AGTTCCAAGACAATGTGGCACTTTATCCCAAAAACAAAATACTTGACTACATAGAAATTAAACTTGTCCT TGCTTGTCTAGGCATCAACAAATTTTCAAAGGAACACATAGTCTTGGGGCAAAGATCAAATCCAGGGTTC ATTAGAACAACCTACTCTCAGGTTATAGATTATATGACTTGTGTATCTGAATCTCTCATTAAGACCTGAG AAAGTTTTAAGGTCATGTCTTGCAGATCTGACCAAGCAGTCTAAAGGTTACATAGTACCATATTGGTTCT TCTATAGTGTTCAGATTCACAGACAAAGAGGAACCAGTCTTGGGGGTGATGTGAATATTGTCTCTTAATG GAATGAATCTCAATAAAACTCAAAGGAAACCTCAACATTTAAAAGATATTTAAACATAAAAGAAAAACAA ATAAGCAAATAAAAAGAATAACAGCTATATAAAAGAACGTCTCTAACTCTAACAGCAGGAAGTATACAAC TTAATTGAAAATGTGTTGCTATGGAAAAAAAGAGGAAGGGTCCAAGAAGGAGCCAAATATCCAAAAGTGA AGATAAAGGACAAGAAACCTATCCTCTGGTGTTCACCACTCAAGTCAGTAGTATTAACCTGGGAGAGCAG AACCTAAAAATGAGGCAGAAGAAAAGTCCTGTACAATGGCCAACTTTATTCAGAATATCAGAACAAATTT ATGTTCTAAGAGTTTAAAAAGAGTCACCTGAGTAAAGTGTCCAATTACAAGAACAAGCCACAGGTGGTAA AAATATCTTTTTCCATAGAGGCATATGAATGACAACAGCTGAAGTAAACTGACTCCTATCTGCTGCACCT TAGAGGAGAATGAAGAAACATTCTGAGAATTCTGTGTTGTGAAGAAACTGAGGTCACAGGACACATGTCT TGTTTCCTTGGAGTTTTCTCATATGTGCTCAGAATTCCCCTCACGGAAGTCCATTCTTGGACTGTGTTCT GATTCTCTGGGAATAAGCCTGAACTTTTATAGGCCTACTTTCTGCTTTGAGGGTAAAGAGAACTAAGAAA ACAGGCCTGACTGAGTTTTGAACTGCTAGGAACCAGCGGCTATCACCGCCCTTTTTTCTCAGTTTTCAAT GGCACTGTCTATTGTAATTCTCCTGTTCATATCATCTCACTCTACACTGCAGGCAAGAGAAAGTCGCTAT ATTTTTAGGCCAGAGAAACAAACAGCACCAGAAAAAGTGTATCCATAATTTAAAGATGTAATTTAAAATC TTCAAGTCTGAGCTACAGACAGCAATATAATAAGCCATGGGGTGAGAAGTGGCAAAGTGTCAAAGTCCAC CATTGCTATCATTGAGATATTGAACATTATGGCTAAGTCAGTGTCGGCATGAGAAAAACAAATTAAATAT AACTCACACTGACTTACACATTGGTTATCTATACAACTTAAAAAATGGAATTGTCACCAAGTAACATAGG AAGGACTATGGGGACAGGTGGATTATGGAGTAAAATTGAGAATCCAACATGTTAAACATGTACTCAGGAA TGATGTGTAGGCTTCGAATAGGAGTCTGGAGTTCACATGGAAGATCATGACTAGACACACAAATTTATGA ATCACCAGCATATGAAAGCAGTAAAACCAGTGAGCCAGGGTGAGATCACCAGGAGAGGATCCCCAGGTTA GACACCAGCTCTCAACTACTGGTTGAGAAATAAACAACTAGTAAAAGAATGAAATGGTAGGTGGTGAGGT AGTAGAAAGACACAGATGGGGATAGAGTAGGAAAGGGCCCATGGGAAGAGAAGCAAGAACTACCCCATGG AGTTAACCACAACTCCATTCTTGGACTCTTTAACAAGAAGTATATTTTTAGGGAAGAAGTGGGCATATGT TGGTTGCAGTTTCCTCCAAGACTCAAACCTTTTATCCTCTAGAGAATCTCCTTAAGTCACAAATTAAACA ACTCAAAATTGATTCAGGAAGTCCCTGAAACTCAGAAGACTCACTAAGAATCAAGACTGCTGAGCTTTAG TCTTTAGCCAAGTTGCAAAGACAAAGGACTCTGAGATTAGACCAGCTGCCTAGAAGGATCAGAAATCAGA CATGCTGCCCACAAGAGTCTCAGATCAACCTAATCACCTGGAAAGGGGGCACACTTTAACCTTTTGAGCT GCGGGCAGGCTGTGCAGTGTTCTCTAGGTTTCCAGGTTTTATGAGCTGTCACCTATGCTGAAGTGGGCTT TGATGATGCAGCTGCTTTTGAGTTATTTCTGCTCTTATAAGTAATTCTCACTCATAGTTCTGTAAGCAAC TCAATTTTAAAAGTTGGACTTTGGTTGTTATGGTGGTTTGTATATTCTTGGCCCAGGAAGTGGCACTATT AGGATGTGTGGCCTTGTTAAAGTAAATGTGGCCCTGTTGTAGTAAGTGTGTCACTGTGGGGGTGGGCTTT GAGATTATCCTCCTAGGCCAGTCTCCTGTTTGCCTTCAGAAAAAGATGTAGAATTCTCAGCTCCTCTATT GCCATGCCTGCCTAGCTGTTGCCATGCTCTTGCATTGAGGATAATGGACTAAACCCCTGAACCTGTAAAT CAGCTCCAATTAAGTATTGTCCCTTATAAGAGTTGCCTTGGTCATGGTGTCTTTTCACATCAATGAAACC CTAACTTAGACAGAGTTATCCCTAGTTTGGTAGATTGTGGGTTTCATATGCAGGGTGAATAATGGTGTGT GTGTGTGTGCGTGTGGTCTTGTATCTCTTCAAGAAAAGCCTTCTCACACAGCAGCATAAAATTCTGGTTT GTCTCAAACAAGTCAATGGCCTTTCTCTATACAAAGAATAAACAGGCTGAGAAAGAAATTAGGGAAACAA CACCCTTCTCAATAGTCACAAATAATATAAAATATCTTGGCGTGACTCTAACTAAGGAAGTGAAAGATCT GTATGATAAAAACTTCAAATCTCTGAAGAAAGAAATTAAGGAAGATCTCAGAAGATGGAAAGATCTCCCA TGCTCATGGATTGGCAGGATCAACATTGTAAAAATGGCTATCTTGCCAAAAGCAATCTACAGATTCAATG CAATCCCCATCAAAATTCCAACTCAATTCTTCAATGAATTAGAAAGAGCAATTTGCAAATTCATCTGGAA TAACAAAAAACCTAGGATAGCAAAAACTCTTCTCAAGGATAAAAGAACCTCTGGTGGAATCACCATGCCT GACCTAAAGCTTTACTAGAGAGTAATTGTGATAAAAACTGCATGGTACTGGTATAGAGACAGACAAGTAG ACTAATGGAATAGAATTGAAGACCCAGAAATGAACCCACACACCTATGGTGACTTGATCTTCGACAAGGG AGCTAAAACCATCCAGTGGAAGAAAGACAGCATTTTCAACAATTGGTGCTGGCACAACTGGTTGTTATCA TGTAGAAGAATGCGAATCGATCCATACCTAACTCCTTGTACTAAGGTCAAATCTAAGTGGATCAAGTAAC TTCACATAAAACCAGAGACACTGAAACTTATAGAGGAGAAAGTGGGGAAAAGCCTTGAAGATATGGGCAC AGGGGAAAAATTCCTGAACAGAACAGCAATGGCTTGTGCTGTAAGATCGAGAATTGACAAATGGGACCTC ATGAAACTCCAAAGCTTCTGCAAGGCAAAAGACACAGTCAATAAGACAAAAAGACCACCAACAGATTGGG AAAGGATCTTTACCTATCCTAAATCAGATAGGGGACTAATATCCAACATATATAAAGAACTCAAGAAGGT GGACTTTAGAAAATCAAATAACCCCATTAAAAAATGGGGCTCAGAACTGAACAAAGAATTCTCACCTGAG GAATACCGAATGGCAGAGAAGCACCTGAAAAAATGTTCAACATCCTTAATCATCAGGGAAATGCAAATCA AAACAACCCTGAGATTCCACCTCACACCAGTCAGAATGGCTAAGATCAAAAATTCAGGTGACAGCAGGTG CTGGCGTGGATGTGGAGAAAGAGGAACACTCCTCCATTGTTGGTGGGATTGCAGGCTTGTACCACCACTC TGGAAATCAGTCTGGCGGTTCCTCAGAAAATTGGACATAGTACTACCGGAGGATCCCGCAATACCTCTCC TGGGCATATATCCAGAAGATGTCCCAACTGGTAAGAAGGACACATGCTCCACTATGTTCATAGCAGCCTT ATTTATAATAGCCAGAAGCTGGAAAGAACCCAGATGCCCCTCAACAGAGGAATGGATACAGAAAATGTGG TACATCTACACAATGAAGTACTACTCAGCTATTAAAAAGAATGAATTTATGAAATTCCTAGCCAAATGGA TGGACCTGGAGGGCATCATCCTGAGTGAAGTAACACATTCACAAAGAAACTCACACAATATGTACTCACT GATAAGTGGATATTAGCCCCAAACCTAGGATACCCAAGATATAAGATATAATTTGCTAAACACATGAAAC TCAAGAAGAATGAAGACTGAAGTGTGGACACTATGCCCCTCCTTAGATTTGGGAACAAAACACCCATGGA AGGAGTTACAGAGACAAAGTTTGGAGCTGAGATGAAAGGATGGACCATGTAGAGACTGCCATATCCAGGG ATCCACCCCATAATCAGCATCCAAACGCTGACACCATTGCATACACTAGCAAGATTTTATTGAAAGGACC CAGATGTAGCTGTCTCTTGTGAGACTATGCCGGGGCCTAGCAAACACAGAAGTGGATGCTCACAGTCAGC TAATGGATGGATCATAGGGCTCCCAATAGAGGAGCTAGAGAAAGTAGCCAAGGAGCTAAAGGGATCTGCA ACCCTATAGGTGGAACAACATTATGAACTAACCAGTACCCCGGAGCTCTTGACTCTAGCTGCATATATAT CAAAAGATGGCCTAGTCGGCCATCACTGGAAAGAGAGGCCCATTGGACTTGCAAACTTTATATGCCCCAG TACAGGGGAACACCAGGGCCAAAAAGGGGGAGTGGGTGGGCAGGGGAGTGGGGGTGGGTGGATATGGGGG ACTTTTGGTATAGCATTGGAAATGTAAATGAGCTAAATACCTAATAAAAAATGGAAAAAAAAATTCTGGT TTGTTCAAAAGAGTCTAAGAATAGGGAAATCTCAGATACATTTTTTCTATATGGTGGGATAAATGACAGA TATAGTGAGAAGGAATAGAATTTAAAACATTAGGCTATGTATTTCTTAATAGGAGGAATTGGAGCAGAGG CATGATGGCAGGAGCTCAATCTATAAGACATGATAAGGAAGCATTGCCCATTCAAGCCATACATACATTC AGTTCCACTGATCACCAGGAGACCCTCCACAGCAGCCAAACTAGGTCCATTACAGCTCCCACTCTGCCCA GTTAGAAAGAAGCCCAGGGGAGAGGTTAAACTAGATCAGGCATTTCTCTGAGCACACTATCACTTGACAA AGGGGAGTAAGACACAATAACTAAAGGAACTAAAAACCTACACTCACTATGTGTAGAAGGGATTTGACAG ATGAGCCTGGTTCTCCTTTCCTGATGCCTGAGGAATGACCCCCAACACACACACACACACACACACACAC ACACACACACACACACACACACACACACACCTATAACCCTTTCCCAACATAAACCATAAAATACAATTGA AAAAACATACATTTATGAAGAATTTCAGCAGAGAGACAGTTCCTTGATCGCCTCAAAGCTTGGTCATGAT TTCTCAAAAGCTGTCAAGTCTTTCACAGTTCAACTGGAAAGTTCAAGAGTAAGCTGTCCATCTAGAACTG TGACTCAATGTTATTGGCCGCCAACAACATTGCCAGGGCCTCTTTCTGTTCACAACAATGAGTTCAGCTC CCTTCCCCCACCAGTCCTGTGGTCATTTCTTCCCTAGGCCCCACCCTTGGCCATTTTCAGTTTCTATTTC TTCATTTGGCAAAATAATTTTTTATCATCATCCTAATGTGGTCTGTGACCTCTCAAGAAGACAGAAGAGA TCAACATTTGGAGAAAACACCCATCTTTTACACTCAGGTACATAGATCACCATCATACTGTCTAGTTCCG TGCCCTGTAGCGGGATAAAGCACTTAAACACACATGCGTACAACACTCGAGTCTCCAGACAGAGAATGTG TATGATCACTGCTAATCAAATGGCTCAAGTTACCTCACTTTGCTAATTCTGACCTCAGCCACTCAACTCC TTAATTTTCTTTTGACTTTTATCATCATGGTGTGTGTCTGCACGGTGGCGGTGACGGGCTCATGTGGCAT GGCACATATGCAAGTGAGGTCAGAGGACAACTTTATGGAATGAGTTCTCTTTGCACCTTTGAACTCAGGT CACCAGGCTTATGTAGCCAGCTCCTTTACTCACTGGCCAAGCCACCTCACTGGCCCAGGATAACACTTTA GAAAGGGATAACATTGTTTAATATACAGCAAAATAATCAGTCAAATGATGGCCAGTGTCTGCTTCCTGGC AGAGATGGTTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTCCTTTC TCACCATACTAAACACAAAGAACATGACAACCTACAAAATGAGCATTGGTAAGAGTGAGCCTTAAGCTCA GCAGCAGATCCTACTCCACTAAGGAGGGACGGCAGCACCACCATTACCGTTTTCCTTTCAGTACAGTAGG AATAAACGTGGAATGGCATTAGAATTCAACAGGACTATGCCTAAGACAAGCTGGCATGGTGCCTTCCTCT TAGTACTTTCAGGTTCACACTAGGTTCCTTCTGAACATGTTGTTTTACTTCAATATGTTAGGATTTCTAG GCTAACATTGATTTTTCCATCCAACTCTAATCCAAAAACAATTGCAAATAGCCCTTCTATCTCCTGACAG CTGCTAACATATTCACAAACACACATATACAAATCTATAGGATATATCAATATATGTATAAATGCATATG TAATTTTGATATTAGTTAACTGAAACACCATAAAATAATTCTTCATCGATTGCATGCCATTTCTCCCTAA CAACACTCACTTTCACTCAACACCCTGATAGACTTTTTCCTCCCACCAGTGCAAAGGCAAGCACAAGCCT CACACAATGTTGAGTTGCAGCCTCAGGTATGATGTAATGGGAGATTCTTCTCCTCCTATCTCTCTTTGAG TCCTAGCTTCCTGTGTGGATGGATGGAATGAGAAGCAGAGAGATTCAGATACAGAAGTCAATCCAGATTT ACCCATTATGAGTCATTATTTTAAAAATCTGATACATATTAGGCAGCTATTCCCATTTAGAGATCATGAA TTTCTCTAAGGGATCAAATGCATACAGATATTATGTGATTATAATCCTTAAATATATAATTAATAATATC AGCTATAAAAGTAATCAGAAGCCAAAATTCTAGCAACATTGGCCCAAAATAAATTAGTAACTGTCACTGT CAAAACTGTGGGGAAGCTGTTGAATAGGAAATTCTTTCATTTTTATCAGCATTGCAATCTTAGCTCTGTC TGGGGTGGAAGAGATTAACCCTCCCAAGACCTGTTTCCCTGCAAATGAGTTACTCTAGCAAATGTGCCAT ACACACGAATATTTATAAACAGATGTCTAGCATGACACTCTCTAAACACATGTGCACTTAGAACAATATT GGGGGGTTAGAATTTAAGTTGTTGACTGTTATTTATGTAACAGGCTGTGGGATGAAGACATTATTTCCTG GGTTGTGGTTAACCAGCATTCAGAAGAAAAGTTACATAAACAGAGACCAGATTCTAAACGTTTTAAAGCC TTGGGTCAAATATCCATTTATAAAAGCTGATTGGTGTTACAAATAGTTCCCTCAGGAAAATCCACAGAGC TCCTTTTTTTTTTTTTTTTTTTTTTTTTTTAGCTTCCCTGAAATCACAGAGCTGAAATCACAGAGCGGCA GCACACACCTCAGATGAAGTGCTTCCCAAGGTCAAAATTCTGCTTCCAAATTGTTTCAATAACCTTAAGC CATTTTGCTAAAACTCTTCAAAATTAGTTTATATATATATATATATATATATATATGTATATATATATAT ATATATATATATAAATCTGTGTCCTTGACAAATGTTATATTATGCAATATAGCCACAGACACTTCTTAGA ATTGAAACAAGATTTAACTAAGAAAGCACCATTGTATTTTTCCTTTAACCCACCAGCAATCTGCAGGAAA ATTCTAAGAAGCAGCTTCCCACCGCACTGTACACTGCCTCTGCCTGCCAATCAGCAGCCAGCAGTAATAT CTAAACTACTAGAAACCTGTCAGGGATCTGAACTAGGGAAAACCTGACCCCCTATCATGTTGCAAAACAG ATCACAAGGCAAGAGGAGCCATTAGAGTGAGAATGGGACTGCAAACTCCCAGAAGGGAAAAGGGGAAGCA TAAAGATCAGAGGAGAAGCAGACACATTTATAACAAGCCTGAAGCAATTTCTGACCCTTCCACATTTTAA GTAAAACTCCGGAGTTTGTTGTTGTTGTTGTTGTTGTTTTTTTATTCTAAAAGCCTAAAATAACTATAGT TTTGTCTCTACTCCTTTATTAATATTGATGTTCCCTCTTGAGTCGCTTCTTTCTTATCAATCCCTCTAAA CTTCCTTTAAGAAATCCTTTAAATTTTAGTGAGCATTCAATGTTGATAAGGATCTAACTTGAGGCAGCAA TCGTAGTAATCTGTTTATTTACCGGCTCAGACTGGAGAGTCCTGGCAGGTCAATGGGGCCTCCCTGGCGC CACCAATGAGAGTATCAGAAATCAATTTAAAATCACCAGTGGAAAGTAGCTGCTGCCAATCACCTCATTT CCATTTCAGCTTCTGCGGGACTCTGTCTCTGGTTCTGTCTGAAACCTGTCAGGACGCTGCCTGGTTCTGT CTGGAACCTGGATCATTAACATTGTTTGGGCATTAACATAATATTAAATGCAGGGGGGAGGGGCTATAGA CATTCTTTTGAGCTTCCCTTTCTTGCAACTTTGCACTCTCCAACCCACTTGAAAAAGTTAGCTGGGTTTT CATCTAAAGCCCGCTTGAGTGAGGATTTTATTTCAGGACAATCTTCCCCGTTGCTTCCCTTCAATCTGGT ATTTCATACAGACAACTGCTTTGATTAAGCCTTGAGCTAAGCTGTTGCTAATACAGACCCAGACCTATGG CCATTCCTGCACATTTATTTCCCTAGAAAACGCATCTTCAACAAGGAAAAGGTGAGTAATTTTTGCAAAG GGAGAGAGGAGAGAGAGAGAGAAAGAAAGAAAGAAAGTCCATAGCAGCCATCCATTCGCCGGGGCCAGAA GTTTGAAAGAATGTGGCAAGCAGAGCCGAGTTAGTACCTTTCCTGACCTCTCTGAGCTGTTAGATGTGGA ACTCAGCCTTCCAAACACAGCAGGATCCCGTCTTCGTCCAACTCTGCAACTGGGCGGGGGCAGTCCTGCC CTGGCAAGGTCCCTGCAGCGCGTAAGGAGATGCACTGCAAGTCTTTTGGGCGCAGAGAATTGGGAGAGAC TTGCAGGAGGACCTGCAAGCTGCCCTACTCTGCAAGGGGGAGGATGAAGAAGAGAACTCTGGGGAAGAGT GGCTGGACAGAAGGGGAATACAGTGCCACTGAGTATTGAAGTCTTGGTCAAGA >gi|363000984|ref|NP_001241676.1| interleukin-15 precursor [Mus musculus] MKILKPYMRNTSISCYLCFLLNSHFLTEAGIHVFILGCVSVGLPKTEANWIDVRYDLEKIESLIQSIHID TTLYTDSDFHPSCKVTAMNCFLLELQVILHEYSNMTLNETVRNVLYLANSTLSSNKNVAESGCKECEELE EKTFTEFLQSFIRIVQMFINTS Vaximmutor Il17f Mus musculus 257630 119360346 AC159614 NP_665855 10090 1 20777145 20784269 - interleukin 17F 8.94 16848.95 161 Also known as C87042; IL-17F >gi|372099109:20777145-20784269 Mus musculus strain C57BL/6J chromosome 1, GRCm38 C57BL/6J ATTTTTGAATTAAAAATATTTTATTATATAATTAATGGATATATTAATTTTCTACTAAATTGATATATAA TCAATAGGTCATAAATATATGTATAGTTCCCCTACCAACATAAATATATAATAGTTTATGCCAACTTCAT TTATATTTACCAAATTTTTCTCTAAAAACACACATACCATGTGTGCTTCTTCCTTGCCAGTCCAGCCCCT GCTACACAGGCCTCCTCCCATGCATTCTGATTATGTTTGTTTTCTTTCCATCACAAATATGAGCATTTCA TTGAACTCTACAAGAATGGTACTGCATCAAAGTGACAGTCTTGACAGACAAGAACTGTGGTCATCATCTA ACACAAAGAGACTGGGCCAAGGGTGCCTTCATTTACCTTTGTTTTTAGGAGTTTAAGTGAGGCATTGGGA ACACACCAGGACTGGTAACAATTCTAAAGCAAGCCAGAAAGTTCAAGCTATATCTACTGCAGAGCTGCAA GAATGCCATGCACACCTTACTGAGAGTCCTAGAAAGCATAAAGACTTGGAGATCGGGCTTCACACAGAGC TCTGGATAAAAGGAGGCTTTTTCTCAGTGGAAGTCTTCAACTGATTTTTGTATGCAGCGTTGTCAGGCCG CTTGGTGGACAATGGGCTTGACACAGGTGCAGCCAACTTTTAGGAGCATCTTCTCCAACCTGAAGGAATT AGAACAGCCCTGGGGCTCCCTCCGAAGGACCAGGATTTCTTGCTGAATGGCGACGGAGTTCATGGTGCTG TCTTCCTGACCCTGGGCATTGATGCAGCCTGAGTGTCTGCACTGGGCCTCAGCGATCTCTGAGGGGAACC GGTGGGGGTCTCGAGTGATGCTGAAGGAAAAAAATAAAAAGTAAAGCAGGATGCTTGAGGCCAGGGAGAG GAATAGAGAAGGGTTTCCGCAGCACTAGGTGTTTCCCTTCAGGGGCAGTTCTGAGTTATGCAGGGAGCAG CGCAGACTCTGAATTCTTGGACCAAGCTCTAGCTGATAAGTAACTTCCTAGACCTCAGATGCCTCCACTA TAAAACGGGGTTTCTCCCATATCATTCTCTCAGAGAGTTGTTGGGGATATCACATGGTTTGAATGCATAC AGAGTAACCCCATAAATACTTTGGTTACTCTTAAATCTACATTTTCTTTGCATTAGTGTTATTATTCATC CTTTAAGCAGAGGTTGAACATCACTAGTAGGTCTTCCAAAGTACAATGCATAAAACTCACTGCTTGGAAA GCAAAATGAAAGCCTTCAAGGTCTGTGAATCCAGAGCCTCACTTGGTTCCTCTCTTGAGACTCCCCTCTG GCTGAAGACAGGCAGCCAAGTGAGTCTACCACAGTCTACAAACCTCTCAAGAAGGGAGCTATGTATCAGC CTCAGAGCACTGCTTTGTCCCTAGAATCTGTTTTTATTCGTGTATTTGAAGAGTATTGATGGGTGGTGAG TGTTCCCAGGACACAAGACTCTGCCCTATACTACATCTATTTCCCAAGCTGACTTTTCTTCCAAATCCTG ACTTACCATCCCTACTCAAAGAAAATGTCTAATCTCAGTAGATCCCTAAACGGATTCATTGTTTCCACAT ATCCTCACAGGCTGAAAAAAATATTAAGACTTCCAATCACATAGGTGACCCAAGGCAAGAAACTGAGTTA GTGGGAAAGGTGCAACTTCTATTGGAGACGGTGAATGTGAAGCCAGGCCTGGTGGCTCATGCCTGTAATC CCAAAACTCAGAAGACTGGGGCAGAAAGGATAACCATGTATCTGAGGCTAGCCTTGGCTACCTCATAAGT TTCTGGGCTACAGAGTGAGGCCTCTATCTCAGAAAAAAAAAAAAAAGTGAATGTGAGTCCTGAATACTCA TAAGAATGAGGCATTGTAATCAAAATAATGTAGCATGTTTTTCTTTCTTTTCTTTTATTATTTTGGTTTT TCAAGATAGAATTTCACCATGTAGCTCTGGCTGTCCTGGAACTCATTACATAGACCAAGCTGGCCTTGAA ATTAGAGATCTGCCTGAGTCTGCCTCCTAAGTGCTGAGGTTAAAGGCATCCACCATTACATTGGGTTTAG CACATTCCTTTCCATGTAATGTGTCTATCTCTCTTCCTTGACTTACTTGTAATCCCATGGGGAACTGGAG CGGTTCTGGAATTCACGTGGGACAGAAATGCCCTGGTTTTGGTTGAAGATTCGAATGTCAACTCTCACAG TGTTATCCTCCAGGGGAGGACAGTTCCCAGCCTTCTGCAAGGCAGGAACCCCTGCTTTGGGGTTCTTCCG AGCTGCTACCTCCCTCAGAATGGCAAGTCCCAACATCAACAGTAGCAAAGACTTGACCTGGAAAATAGAG ATTACCACAGGTTAGAGCCCTGGTGGTGGGCAATGTACTTGAACTCTGTATAGGGTCACTTTACAGGATC AAGGTTTCTTTTTTTTTTTTTTTTTCATGGTTCACTAGCAATGAGAACTGCCGTGTTTAATGTTATCAGG ATACAAAGCAGAAAGGGTCAGGAGTAAAGCTCTCTGGAGTTTATCTCCGGCCTTATAGTCCACAAAACCT TTCTCTGGGACATTCCACCAAACCTCACCCTACTGTCAAGGTCCCATATGTAATTCAATTAGAAGTATAT TTATTGGGATGGAGGAATGGCTCCACAGCTAAGAGCACTGATTGCTCTTCTAGAGGTCCTGAGTTCAGTT CCCAGCAACCACATAGTGGCTCACAACCATCTGTAATGGCATCCAATGCCCTCTTCTGGTGTGTCTAAAG GCAGAAACAGTGAACTCTTATACATACAATAAATATTTTTTTAAAAGTATATTTATCAACATTAAATAAT AAAGATTTTAGCATGTATCACTCAAGAAGTATTTTTTCTGTTCTCTAAAGATGTCATATCTGTGTTCATA CCAGACTCAGAGTTAACTTCCTGCGAGAGCATAGTTGGTCCATGAAAACTTTCTGAATGAATGAGAGAGT GGGGAAGTGAATGAACAGGGCAGCCCAGTTAAATTAACCAGTATAGTTATGCCACGCTCATTGAGTAAAA GGATTATGGGAAACTAAGCACAGCAAAATTTCATTTATCCATGCTAATTGAAAGAAGCAGGGACACTGAT GACGCCTGGCATCACTCAGAGTGCAGAACCCATTTCTTTTAGCTCTAGAATCTATCTTTTCTGTACTAAC ACTGAAATCAGTTTCGCTGCTGTTACTAGCGCACACAAAGTGCTCAGTGGAGCTCTAAACACAAGAAGAG CTAAAACCTCAACAGCTCCCCTTGGGTGCAAATGTCCTTTGCCGTCCCTGCTCACACCTTCCTGGGGAAG AACAAAGCCCATGGTAAGGCAGAAGAGCTAAGGCTGAGCTTTTCATTTCTGGGCTCCGTGTTGTTCAGGC TCACACTGTGTAGGTAGGGAAAGTGACTGTGCATGTTGGATTTAAAGATTTGCCTGTTCTTTTCAACATC CTGGAGGCAAGAAATATGCAGAATAAACAAAGAGGTCATGGAGTCAGCCTGCTGGACTACGATCACTGAT AATACATTGAAAGCAAGCTGCAGGATGAAACAGATTTGTCTCCAGATAATCAGCAGCCATATGCCTTCGC CTCTGAAGTCATTCGTTCACTGAGTAGCTAATCCTCGAGGCCACAGTGTTGTGGTTTCAGGCATTATCTC TAGAGTTAACTGTGTGGAGTCCATAATTCATTTATAGGACTCACCGCACAATGACTTGGTTTCCTACATC CTCATAAATCCCAAAGATCCCTGTAAGCATGCAGTAGTTCCACAAGGCAACAACGGTTGGCAGTGTATTG ATGTTACCCTGTGCATGGGATGGATGTAAAATGCAGTCTTCACAGGTGCTTTGAAATTTAGAAAACATAG TCACACATCTATCAACCACTTCTTTAGATTTCTTAAGTAAATTTCTCCTCCAGAATCTTCTCTTTCTTCT TATATGTCATGTTTATTGTCTCACTTCTCATTTTGACTAGCAAGACTGAAAGTCACTATAGTTCGGGGTT ATGTGATTAATGTAAGTTGTTGTGGTGGTTGAACTGCGAAGCAACCTGCATAGGCTTATTTATGTGGATG CTTGGTCCCCAGTTGGTGGAATTATTTGGGAAGGAGTAGGAGGTGTGGTCTTGTTGGAGGAGGTGTGTCA CTGAGGTGGGCTTCGAAATTTCAAGTCTTATATCATTCCCAGTTAGTTCTTTCTGCCTCATGGTTATGTC TTAAGATGTGAACTCCCAGCAACTTCCCCAGCGCCATGCCTACCTTCCTGCCTCCAGCCATGATGATCAA GCAGCTTAACCCTTGGCAACTGTAAGCCCCAAATTAAACACTTCCTTTTATATGTTGCCATGGTTACAGT GTTTTATCACAGCAATCGAAAAATAACTAAGACAACGATCATGTCCCTAACAGCTCATTATGTTTCTTTA TTAATAGCTAGACATTCTTGGAGTATTTTAGAAAATGTTTTCACACAAGATTTGAACAGCTAACTCATAG ATGAAACTCATCCTAACTATCTAATCAGCTCTCAAAGGACTTATGTCAGAGAGGCAATAAACAACCCGGC AGGGTTCCCCAGCCTCATCGTGACAATCTATAGGATCCACCTTCTATGGCAAGGCTTGGCCACAGCGACT GAAGGTCATTACTATTCTTCACATTAGTCTTCACTGTGTCTAATTTCAAGAGCCACGAATTCTAAACACT CTCTGCACTGCATCAGTAAGACAGAAGTATTGAAAGCCACAAACCACTGTTTGCTTTATGCGTTTTTGGG GTTTTGTTTGTTTGTTTGTTTGTTTTTTGGTGTTTTGCAGCTCTGATAAGGCTGGTGCTGAGTATGGAAA AACAATAGAAACAATCCATCTTCTCAGGGGAGTCGGGAGATTTTACAGGTTTAAATTCCACCTTTAAAGG GGATTGATAGGTGTGATTCAAATCCTGTAGGATGGGAAACAGCTAGGAGAATATTGAAACTGAAGGTGTT CAGTCATAGAGTGTGAATCTTCCCAGCTTAGCTTTGTACATTACTCTTGTGTGCTCGCTGGTGGCAGAAC ATCTGCACACTTGCCCCTACACTTTCCTCCCTACACAGGAATGGGTGACAGAGACTGGTCAGCTCCAAGC TCCATCCAAATTTCCCTCAATTGCCCACAGCATATGTCTTCTCTGCTTGCCCCTCCTCATAATCCCGTTT CAACACTTCTTGCTCCACCATGTTTCTTGCTGACAATCCAACTGATTTTTTCTCCCCCAAAGCAATAGTA ATGGTTTGGAGCATTAAACTATCCAAACGATTTTCTTGGCTACAATGGCATTAGGTTGAGAATCTTGCCT CTCTCGATCATCCACAGATAAGCACACTATTGTTGGGAGTCAGAAAATCTAGATGCAGGTCTCAATATTA TTATATTAGCAATATATTAGCATTGCAATAGAAAATGACAGCAAATGAAAACAGATGACTGAGCTACTGT GTAATGGAGAAAATGTTCGTGATCTATTCCTGTGGGTTGACAAGGGTCTGAGAGGTAATAGTAAATCACA GAGCAAGGCTTGGTGGCACACTCCTGAAGTCCCAACACTCCAAGAGAGCAAAGCAAAAAAGACAGTTCCA GAGCAGTCTGAGCCCACATAGCAAACTCACGACTATCGTGAGCCACACAGAAGATCTTGTCTCAAGGGCT GGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCCTCCAGAGGTCCTGAGTTCAATTCCCAGCAACC ACATGGTGGCTTACAACCACGTGTAATAGTACCTGGTGCCCTCTTCAACAGTGTATTCACATACATTAAA TAAATAAATAAACAAACAAATAAATAAATAAATCTTTTTTTAAAAATCTTGTCTAAAAAAGCAAAAACAA TAATATAAAGAATTGGTATTTGTATACTCTTTCTACCTTTACCTCTTATAGCAATTGCAGTGTCCAAGTT AACTAAAATCTAAGCACTTATGATCTACCTTGCAAGTCTAGATTCATATCAATATAACAATCTCTTCCCC CATAATTATGCACAGGTAGGGGAAGTAAAACTCACAAAGGGAATGACCAACACCAAAGTGCATACTTAGC AGGTAGGAGAGACAAATCGGTCTGACTTCAAAGCCTCTGTCTCTAAATACTGTATTTTGGTGGTTTACTG CAATCACTATAATACTATACACTATGCAGTAATTAAAAGAATATGGCCATTGTCGGAAAGCATGAGCCAT TTATAAAAGCCATGCTCTTTCAGGTCTCAAGGCTTAGCCAGGGAAAATCAAAGGACTATCAAAACCAATA ACACAGTGTGTGATTTCCTAAGCTTACTCCATGTGCCTCTATATTAAACCCTTCCCAGGTATTAATTAAT TAGTCAGTTACCAAAGTCCCGAGACACTTCTCCAGGAGCTGAGCAAAAGTTGAGCCCTGGGCTATTCTAC TTTCTCCTCCATCCAAATGACCTTGGAGGAACCAAACCATGGGTCCCAGCTTCTTTGTGGTATATATTAT ATATTAATATTTAATGTATATTTATACTACTATCGCATGCAATGTAACACGATATTATATATAGATTCCT GCCTTGGAATACATATTTATATAAAACAAAGCTTGGCCTGGCTCTTATTTTAGGTCAACCTAAGTCTTTA ATGAGTAGGTAAGAGAGACCATGATGGTCACTGGAGATAACCAGATACACACACCAGCCCACCTGAGCAA ATCTATCAGGTAAGATCATCTGCTCTTCTCCCATTCTAACTGTTCCATTGATGTTGGTTTTTATTCTTCT CTTCTCTTCTCTTTTTTCTCCACCAGACAAATCAAAATTAAACTTAACTTCACGTTTTAAAGAGCTAATT CACAAAAGACTCTAAGAAGGCCTAAAGCCTTAGAAATCTCCCTTTCTTAAAGCTTAGACGCACCTATCCT TAGCCTATGATTATTCAACACCACTCACCATGGCTGTTTCACGGGTGCACTTCATGCTGGTTTCTCCAAT GGCTGCTTCTTCCCTCAGGAAGTACTTTCTTCTAAGTATGTCTTCCTGGGTATGT >gi|119360346|ref|NP_665855.2| interleukin-17F precursor [Mus musculus] MKCTRETAMVKSLLLLMLGLAILREVAARKNPKAGVPALQKAGNCPPLEDNTVRVDIRIFNQNQGISVPR EFQNRSSSPWDYNITRDPHRFPSEIAEAQCRHSGCINAQGQEDSTMNSVAIQQEILVLRREPQGCSNSFR LEKMLLKVGCTCVKPIVHQAA Vaximmutor IL2 Homo sapiens 3558 28178861 AC022489 NP_000577 9606 123372624 123377649 - interleukin 2 7.95 17130.78 153 Also known as IL-2; TCGF; lymphokine >gi|224589816:123372624-123377649 Homo sapiens chromosome 4, GRCh37.p5 Primary Assembly GTTTATATTTATCAAATTTATTAAATAGTTTTACTAACCAATCTACATAGATACTATATTTAACATTCAA CATAATAATAAATATTTTGGGATAAATAAGTGAAACCATTTTAGAGCCCCTAGGGCTTACAAAAAGAATC ATAAAAGATCCATATTTATAGTTTTAAGATTAAGAATAATAGTTACAATAGGTAGCAAACCATACATTCA ACAATAAATATAAAATTTAAATATTTAAATAAATAGAAGGCCTGATATGTTTTAAGTGGGAAGCACTTAA TTATCAAGTCAGTGTTGAGATGATGCTTTGACAAAAGGTAATCCATCTGTTCAGAAATTCTACAATGGTT GCTGTCTCATCAGCATATTCACACATGAATGTTGTTTCAGATCCCTATAAAAGAAAAATGTTAATTTTTT AAAGTACAGAGTAGTTTACCTTATATACAGTTATTCCCAATTGAAGTCTTATAGGCCTGTTGCCTTTATT TTCAAGCTTACCAAACATATTAATTATTCACATTTTCTTGAAATGATCAAAATGAATGCCAAAATTACAT ATTTTGTTATGATACCAAATGATAGTAACACAGAAGCTGAGATTTTCCTCATTTGTTAATTCAGTCAATG AACACAAAAATTTTTTCCCTAAATTGGGTGCAAATAAAGATTTAAATAAAAATAGATCTAGACAAATATT AAATGCTGGATGTGAAATAAACTTGATCGCTTTTCCTCTGAATGTACACCTATATTTGTGTAAGTTAAAT GTATGAATTGATATGCACATTATACTTTCTTCTTTTCTAAATCTTGATTAGAGTCTCCTATTTTTCTACT TAAGTGAATCTCCTTATATTTTCCCCAAAGCAGAACAGAACTACACTAGAGTAAGCTAGGACATGCTTCT GTTTGGGATAAATTTGTGTGAAGAACATCTTCATAAATACTTACACCTATCCCTACCCCATCATAGTATC AATGCAGGTGAATTTGCCAACTTTTAAAAGTTTAAACATTAGAAATTTTGTTCTGTGGACTGGCTTTTTA GAAATTAGGCTTCTCTGCTGAAAGGAGCTATTTAAACGCTTCCTCTAGTTGATAAGCTAAGGAGGAAATA TTTTGATATATAGTGAGATTTAAAAGAGAGCTTTTCAAATTTGAAGTGATTCCTACCCTGGGAACAAAAG CAGGTGAGATAGAGTGTTCCCTTTAAATAAGTATTCTCTTTTAAAAAACTGTTGCAGTGTTCTGTATCCT CTTAAGCATAATTGTTTAAATATTGTCATATCTTGGGATTTTTTTATTTTAAAAAGTGTTTGGCTTTTGA AACACAGTTTTCAGATTTTTGCATGGTTTTGTCTTAAATTTGTATCAAGAGTTAAGAATGAGTTTTCTGA TGTTAATTATTTTAGAGTAAAACTCTTGCCATAGATAAGGAACTGAATCTGTCAATGTCTAGATGACATT GATCTCAATGTATTTTTTTAATTCAACTGAAACAGTCCACTAATAAGTGATTCATTTGGACACAACTGGA GTTACTTTTAGGAATGTCTTTTACTACTATACTTATGCTGCTTATTTAGGATACCTATTAACTCATGAAT TTTATACCTTAGGAGACGGGATATAAAAAGACAAAAGTGAATCTCTTTTACATCACCTGAGTCCCTTGCA TATTTTATGCTTCCTGTGCATTTAAAAATACTTAAGATTCTGAAAGTGGGGATTGCCTCTAAAAAGATTG TTGTTTGTTTAGTCCACTGGCCATTTTAATGGCTCCAGTTTTTTTTTTTAAGACAAATCTCTTATGGGTA GTCCTTCCATCAAATGATAAGGAAACACCATTTGAAAAGCCTTTCAGGTAATCAGTATTGGCTATTCCAG TAAATGATCTGAAGGAATTGAAAAATATATAGTAAAATCTGCCTGCTTTCTGTGAAACTCAACTCAGTAC CTTCTCTCACTCCCAGTTTTGTGTTAATATGAAAGGTGATGGAGTTTGGGATGAGGCTTGTCCATTAGTA GATGGTAGGTGCAAACTCAGTAGCTTATACTCCCCAGTTAATCATTTAACATTAGAAATGATTCATGTTC ACAGTGTACTTAAAATGTAGGCTAATTACATGCATGGGTACTTTACAAATACCAAAAATGTTATGTCACT TTAAAATGTGGTACTTTTCCCCCTACTTTTTTTTTTTTATTTCCAGGAGAGCAAATAAAGTAATGCCTTA CCTTTAGTTCCAGAACTATTACGTTGATATTGCTGATTAAGTCCCTGGGTCTTAAGTGAAAGTTTTTGCT TTGAGCTAAATTTAGCACTTCCTCCAGAGGTTTGAGTTCTTCTTCTAGACACTGAAGATGTTTCAGTTCT GTGGCCTAGAATAATAATTATCATCAGCTCAGTTTACATAGAGGTCAGAATCAGAAATTAATTTCCAATT TATTTTATTTGGAGTAGCAGTATGTAGTTTTTACCATTCTCTCTGTTCTCAATGTCACTGTAAAGATGGA CCAGTAGAGTTTACATTATAAAACTGGAAAAACTAGGGCTCAAGTACCTATGACTAGCGTTAAGTGATAG TTGACTCACTGGTTTTAGCTTAATTTGGCTTTGCAAAATGTTAGACTTTCTGCATCTAAATGCCTATTAC AGATGTAGGCTGATTACTTTTCCAATCTGCATAGAAAATCATTAGAACCAGATGAGTTAGAATGTGGAAG CATAAAAATCACATTCTATGATTATAATATTTGATAGTCTAAGAGTTAAGAATCACAGAATGTTGAGAGA GAAGGAACTCTTGGATGCCATCTATCACAATCCCAACATTTTACACTAGGTAACAATAATCATGTAGTAG TGGCTCTCAATATAGCCTTGAGCAAGACACATTTTTAAATATGTCTTAAATTTTTACTTTTCTACAATCA CTACATTAAGATGCATTTTAAAAATGTTTGTCCCAGTGAAATAAAAAATTATAATACTTTGGGAGGACTT TTGGACAGGCCTCTTGATTCTATATTATATATAATATTTCATATTTCTATGGTATATTACAATTTAAAAG AATTTTCTCCAAAATAGGCCCATTTACTTCTCACAGTCATCTTTTGAAGTAGGCAGTGAAGTATCATCTC CACTTTATAAATAAGCACACTAAATCTCAGAATTTGACCACATAAGTAGGTAGAGCCAGAATTAGATTTC ATTTCTTTCTCATTATAGCATTAACATAGCATCCTTGGAATCAAATATTTTTATTAGTTTCTAAAATCTT TTGTAAATGTGCAATGAGGGTTAAATGAACAAATGAATAGAGTTTATCATTACTTTTGGTTTCAAACTTA GATTTCCATGAATTCTACCACCCCCTAAATTATGCACATAATCTCATCTTTCTAACACTCTTTAGTGGGA AAGGTAGGTCAAGACAATACCAATCCCCTGATATGTCCATCTATTGCGCTTTCAATTCACCACTACAAAT TCTACAAATTCGGGTTTAAGTTTTTGGCATGAACAATGTGGAGAAATTTTCTTCAGAAAACAGCATCTAG TATCTTTATGTTGCTGGAGTTGCATTATGTGGCTCTGATTAAACTAGAGTAACATCTGTAAGCTGTCTCC TTTACATCTTTCAGAAGGGTAGTTAGAAGCAGATTTCTCTTGAATTAATAGAATTTGAGTCAATGGGATT GCATCTGTGTCCCCTTAAATCCTATTATTTTCTGGGCTTGGATTCACCCTGGCACAAGCTCTTTCTGGCA CCAGATTTTGTTCATTCTCTCTTAACTGGCACAGCTACTAGGTAGCTTTACACACCTAATGTAGCCTAGG AGCTTGGATGATTATGCTTATGCTCTATCTGAATCCAAAAACAACCTAATGAAGCTTTTAATCTTGGAAA ATAAGTCATATAGCATTATAAATCTCAAGTTCTATTGGTAAAAAGATAAAGTCCAAAATTTTACTTTGTA TTTCTCAGTGAAAATTTTATCTTTAATACTACTAATAATAGCTAATGCTTGTATGGTATTTACCTTATGC CAGACACGGTTCTGAGTGCTTTACATTTACTTACTAATTCTCACAAAGCCATATTAAGTATATACAGTTA TTCATTTAAGTAGACATGAAACTGAATCACAAACAGAGTAGGTAATTTGCCAAAGATCATGAAAGCTAGG ACATGGCAGAGCTGAGATTTCACTTCAGATATCTGGCCCCAGACTCTGTGCTATTAGTGTTATGCTGCCT CTCACTTAGAAACCTTTCCTAAAACAAGAAAAGATTTTTAAAAGTGGGCTCCTTGGAAATAGATTTTGGT ATTTTACATACTAAAATGACATAATCAAATATTTTAGAGTTTTACATTTTTAAATGAAACCATAAATGAA CATTTTTGTTTAGAATTGTCTTATCGATAAAGTGAGAATATTATTTTTATGTTTTCTTCCCACCCCCACC AACACACAAATATATATTAAAAATATAGGTAAAACTGTTTTAATAGAGGCTTCATTATCAAACTTGGGTT TTCAAAAAAACAAATTAAAGTTACTCTTTACCTCAGATGAGCTGCTATTAGTCCCATCTGTGCAAATTTT CATATTACTTTGAATTTTATTAAAACAGAAATTGAACATAAAATATTGTACTTACCTTCTTGGGCATGTA AAACTTAAATGTGAGCATCCTGGTGAGTTTGGGATTCTTGTAATTCTAAGAAAGTATAATGCATTGTTAT TAAGAAATGATCTCCAGCTAGATTACTAAATGTAATAATTTTAGTAAGAAAGGAAATATACTTACATTAA TTCCATTCAAAATCATCTGTAAATCCAGCAGTAAATGCTCCAGTTGTAGCTGTGTTTTCTTTGTAGAACT TGAAGTAGGTGCACTGTTTGTGACAAGTGCAAGACTTAGTGCAATGCAAGACAGGAGTTGCATCCTGTAC ATTGTGGCAGGAGTTGAGGTTACTGTGAGTAGTGATTAAAGAGAGTGATAGGGAAC >gi|28178861|ref|NP_000577.2| interleukin-2 precursor [Homo sapiens] MYRMQLLSCIALSLALVTNSAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKA TELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNR WITFCQSIISTLT Vaximmutor Il2 Mus musculus 16183 7110653 DN-144H19.3 AF195954 NP_032392 10090 3 37120712 37125953 - interleukin 2 4.63 18506.61 169 Also known as Il-2 >gi|372099107:37120712-37125953 Mus musculus strain C57BL/6J chromosome 3, GRCm38 C57BL/6J ATTTTTTTTTAGAGGAGAGCTTTATTTCTTGAAAACACTGATTAACATAGAGTTCACAGGAATAACTGAG AAGTTATTTTAGCGCTTACTTTGTGCTGTCCTAAAAATGACAGACATCTGAGCTTATTTATATTTGAATC ATCTAAATACTTTTATTAATGAGTCTACCTACACATGATATTTAACAATTCAATATAATAAATAATTTCA GATAAATAGTTTAAAACATTTTTGAGCCCTTGGGGCTTACAAAAAGAATCTTTAAAGATCCATATTTATC ATCTGAAGACTAGTAGTTACAAAAGATAGTAAACAATACATCCAAAAATAAATTAAAGTTAAATATTTAA ATAAATAGAGAGCCTTATGTGTTGTAAGCAGGAGGTACATAGTTATTGAGGGCTTGTTGAGATGATGCTT TGACAGAAGGCTATCCATCTCCTCAGAAAGTCCACCACAGTTGCTGACTCATCATCGAATTGGCACTCAA ATGTGTTGTCAGAGCCCTAAAAAAGAATATAAAATTGTAGGCTAAGGTAGCTTACTTTGCATATAATTAT TCTTCACGGAAGCTTTTAGGCATTTATTTTAACACTGGTTAAATATATTGAACATTCACATTTCTGAAAT GATCACAATTATTGGGTTATGTTTTATTATGATATCAAATGGTAAAGCATAAAAAATAAAGCTCTCCAGA GTTGATTACCAACGAGCATTTGATGGGAACACTGAACACTTCTGTAGAATTCAGTTGCTTGACCACTTTT TCTCTGGTAAGTGTACACAGCTATGCATCAATTTAGGCTTCAGGAATGGAGAAATGATACTTGTTATATG TTCTTTGCTAAATCTTAAGAGTCTATTATTCAATTCTTTCCCCCCCAAGTCAGAATGGGAACAACCCAGA GTAGGTTAGGACAAGCATCTATTGGGGGAAATTTGGAGAATTTTGTTATAGATATACCTACCCTGATACC AGTGTAGATGAATCACAAAGTTTGTACATGTATAAATATTATAAATTTGTTCTGTGGATTAGCTTTTTGA AAGCTAACCCTCTTTGCTAAAGGAGATATTTTAATTCTGTTTTTAGGTGACAAGCTGAGAAGGAAATATT TTGACAAATTTATGGGATGGCTTTTCCAAATCTGACTAATTCCTTGTAAGTCAAATGAGGCAGATTATTC CTTTTAAATAAATACTCCTTTTCAAATAAGCTGCTGTGGTCTTATATACCCTCTTTACATAGCTACTTCA ATATTCTAATGTCTTGAATTTTTCATTTTTAAAAATATGTGGCCCTTGAAACATTTTTCTAGTTCTCATG TAGCTCTAAAGTTTGTACCATTCATTAGGGGTGAACTTTCTGATGTTGGCCATAGATTTTAGAGCATCCA TCTTTGCATACGTAAGTACTCGATTTTAGAGCATCCATCTTAGCATACGTAAGTACTCACACCTTTCAAC GTCTAGATGATGTGTATTTCAGCCTGCTTTAAAAACCTCAAATGGAAGCTGGGCAGTGGTGGTGCAGGCC TTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGT GAGTTCCAGGACAGCCAGGGCTACACAGAGAAATCCTGTCTCAAAAAAACAAACAAACAAACAAACAAGA AACAAACAAACAAAAAAAACAAAAACAAACAAAACAAAACCTCAAATGGAAACAGTGCACTGAAATGTGA CTTTGGTTTGGAAAGGACTAGCCCACACCCTCTTGGAGGCTTGCTGCTACTGTCTCACCAGAGTCCATGA TTCTTGTGCATTTAAAAACAAAGCTCTGCAGTGGGCTCTCCCCGTCCTGCTCTGTCCATTTTAATGGCTG CCATTTTTGGAGAGAAATGTCTGTTTTTCTACCAATACCAGCACAACTTCTCTGGAAAAACTTTTCAGAT AATTTTTTCTGATCTGATGAATGTAACACCAGCAAGAGTTGCTTGTTTCTTGTGGAATTCTACTCCGTGC TTTCTCTCACATCCAGTTCTATGCTGGTGTGGAGGGAGCAGAGTGTTCATGTTCCCAGTTTCCTTGCAGG TGATGGTAGGTGGAAATTCTAGCATCATCCTACAGTGGAAGGATTCACTTGCACAGTGACTTTAAACTTT GGCTGACTAAATGCACAGAACCCATCAAAGACCAGAAATGGCAAGCCACTTAAAAATGCATTTCTTCTCT ATTTTATTTCCAGATTAGCAAATAAAGCAACACCTTACCTTTAGTTTTACAACAGTTACTCTGATATTGC TGATGAAATTCTCAGCATCTTCCAATTGAAAGCTTTTGCTTTGAGTCAAATCCAGAACATGCCGCAGAGG TCCAAGTTCATCTTCTAGGCACTGAAGATCTTTCAATTCTGTGGCCTAGAGGAGTAATAAGCTTAACCAT CAGCTCAGCTCACCACATACTGAAGAGCCAGAAAGTTAGTCTGCAGTCTCTCTAATCAAGAGAAGGCAGC ACATAGCTTTTACCACTCCCTGTCCTCTGTAAAGGGGGACTGAGGTAATCGATGCCATAAATCTAGAAGA GCAAGAGCTCACAACCATAAGGGTCACCTTGACTGTTAGGCCACTCTAGTGAGCTCTTCTGGCTTCATTA GACTTTGTAAAAAGTCTGTGTTTCTCTACCAATGCATAGCACAAGTTCAGACTATTGTTCCAATCTACAA AGAAATCTGCAAGGTTCACATTCTAATATCTAATCGCAGAGTTGAGAATCACAGAAGGGTAAGGGACAGG AATCCTTGGATGCCAACTGTCATAGGCCTAAATCTTACAGATTAGGTTATCATGGCCCTGACTGAGGGGT GTCAAGATAGCCAGGAAGACACATGTAATATTTTTTAAATTTATACTTCTCTGCAATTACTAAAGATGAG TTTTTTAAAAACCCATATGTCAAGCTAGGAAGATGGCTTTTAAGGTAAAGGTACCTGCCACCAAACTTGA TGATTTGAGTTCAATTGCAGTGACCCACCCACATTGTGGAAAGAATGGCCCAACTTTCATATTTCGTCTT CTGACCTCCACAGTTACACTGTGATACACATGCACCTCCACACATACATACATACATACATACATACATA CATACATACATACATACATACATACATAAAATGTAAAATCTATTTGTCCCAATGAAAAAAGTCATAACAT TTTGGCAGAACACTTGGGAGAATTTTTCTGATCCATGTATGAAATAATGTTTAAATACATCATGCATACT TACATACATATACAGGGAAAAAGGATATTGTCTAGCTCTATAGTACACTATGATTTCCATAAAGTCAGCT GCTTTAAGTCTACAGTGAACTGTGTTATCTCCATTTTACCAAGTCTCACAATCAGTGTCTTTCCCACAAC CACATGAACGGGGAGCAAGGGTGGCCCGGTCGGTCGGTCGCTCCTTTCATTAACCACAGCCTTTGAATTA CATGCTTCTATTGTTTTTAAAATCTTACATACCGAATGCAGGTTAAATGAAAACATCAGCAGAATTTACC ACATTACTCTTGTTTTCAATAATTATTTTAAAACTTAGATTTCTAGGAATTTCACCACTCTCTAAATTAA CATGATCTCTGTTGTTTCTCTAACAATCCTTAGAGGAAGAGGTTTGACAAGATAACACGTAGTTCCTGAT TGGTCTGCTATTGACCTTCCAACTTGTTATGGTGTGTCTTGGGTATTCAGTGTGGAGCTCCTTCCCTCAC AGAACTCTCCAGTTTGTTACTGTGGGAGCTGAACATTATGTGACTGTAATTAAGCTGGAAGAGCAACTGG GGCCTTTCTTTTTCATCTCCTGTAGAACAGCTAGAGGCAAAGTTCCCCTGACTCAATAGGAATGGGTCAA CAGCTGCCTCTTTGTCCCCTTAGATCCCATTTTGCTCTAGGCTTGGATTTACTTGGGACAAGCTCTTTCT AGGACCAAAGTTATCCATTCTCTTTTGACTTTCGGGACTAGAGCAAATTTCTACCCTTGCTGGAGTTCTA AGAGCTTGTGTAAGCTGTTTTGTATCACACAACATTCTGCCTCCAAAGGAGACCAAAAAACCTTTCAATA TTGAGAGATAGTATCATGTAGTAATATTACAGCTCTCTAGTTCCACTAGTTAAAAGAGACTGTATAAATC CAAAATTGTTTTATACATCTCGGACTTCTTCTCTAGCACTAATAGTGTGTTTATGTGCTGTTGGCCAGAC ACTGTTCTGAAACCTGACAAGTGATAAGCATAGCAATGATCATTTATGTAAGTGGGTGAATGAGCATAGA CAACTTCCTGAGGCCATGAGAGCTAGGCCCTAGCAGGGCTGGACTCCACTGCAGATCATTGGCCCTAGAA TCTTAACATTGGTTATGCTGCTCCTCAAAAATGCAGTCATAGGAATCATTCTTAAAACAGTAAAGTGTGT GTAAAATGAGACCCTCTCAAGTCAATTTTTACATTTAAGAAGCTGAAATAATGCACCTCTCTCAAATATC TGTAGGACAAGCATAAAACAGAAATCAACAGTCTTTAAATTATCCCAGCATCAAAATGCAATCATCTTGC ATTTTCTTCTCATTCCCTTTCCCAACACATAAATACACAGGAAAAATGTAAAGAAAACCATTTAAAAGAA GTACCATTGTCAAGATTTTTTTACAGAGAGATATCAGAGAAACTTATTTTCTTCAGATGAGGTACTATTC ATCTGGTCACATGTTATTTACACGTTATACACATGTATGTTACTTTGAATTCATTAGAGCACCAGTTAAA CACAGAAACTCACTCACCTGCTTGGGCAAGTAAAATTTGAAGGTGAGCATCCTGGGGAGTTTCAGGTTCC TGTAATTCTGAGAAAGCGTAACACATTATTATTAAAGGTTATTAAACACAGCCTTTGGCAAGAAAGCTAA AGGTATTGCCTATAGATGGGATGGCTGTGCACTTACCTCCATCCTGCTCAGGAGCTCCTGTAGGTCCATC AACAGCTGCTCCAGGTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGTGCTTCCGCTGTAGAGC TTGAAGTGGAGCTTGAAGTGGGTGCGCTGTTGACAAGGAGCACAAGTGTCAATGTGACACAGGATGCGAG CTGCATGCTGTACATGCCTGCAGGACTTGAGGTCACTGTGAGGAGTGATTAGCAAGGGTGAT >gi|7110653|ref|NP_032392.1| interleukin-2 precursor [Mus musculus] MYSMQLASCVTLTLVLLVNSAPTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKL PRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFEC QFDDESATVVDFLRRWIAFCQSIISTSPQ Vaximmutor Il4 (interleukin 4) Mus musculus 16189 10946584 RP23-188H3.4 NM_021283.1 NM_021283.1 10090 ? IL-4, Interleukin 4 >gi|10946583|ref|NM_021283.1| Mus musculus interleukin 4 (Il4), mRNA GGATCCCCGGGCAGAGCTGGGGGGGGATTTGTTAGCATCTCTTGATAAACTTAATTGTCTCTCGTCACTG ACGGCACAGAGCTATTGATGGGTCTCAACCCCCAGCTAGTTGTCATCCTGCTCTTCTTTCTCGAATGTAC CAGGAGCCATATCCACGGATGCGACAAAAATCACTTGAGAGAGATCATCGGCATTTTGAACGAGGTCACA GGAGAAGGGACGCCATGCACGGAGATGGATGTGCCAAACGTCCTCACAGCAACGAAGAACACCACAGAGA GTGAGCTCGTCTGTAGGGCTTCCAAGGTGCTTCGCATATTTTATTTAAAACATGGGAAAACTCCATGCTT GAAGAAGAACTCTAGTGTTCTCATGGAGCTGCAGAGACTCTTTCGGGCTTTTCGATGCCTGGATTCATCG ATAAGCTGCACCATGAATGAGTCCAAGTCCACATCACTGAAAGACTTCCTGGAAAGCCTAAAGAGCATCA TGCAAATGGATTACTCGTAGTACTGAGCCACCATGCTTTAACTTATGAATTTTTAATGGTTTTATTTTAA TATTTATATATTTATAATTCATAAAATAAAATATTTGTATAATGT >gi|10946584|ref|NP_067258.1| interleukin 4 [Mus musculus] MGLNPQLVVILLFFLECTRSHIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCR ASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS Vaximmutor IL-4 plays an important role in Th2 immune response. IL6 Homo sapiens 3569 10834984 AC073072 NP_000591 9606 7 22766765 22771620 + interleukin 6 (interferon, beta 2) 6.52 22923.52 212 Also known as HGF; HSF; BSF2; IL-6; IFNB2 >gi|224589819:22766765-22771620 Homo sapiens chromosome 7, GRCh37.p5 Primary Assembly CAATATTAGAGTCTCAACCCCCAATAAATATAGGACTGGAGATGTCTGAGGCTCATTCTGCCCTCGAGCC CACCGGGAACGAAAGAGAAGCTCTATCTCCCCTCCAGGAGCCCAGCTATGAACTCCTTCTCCACAAGTAA GTGCAGGAAATCCTTAGCCCTGGAACTGCCAGCGGCGGTCGAGCCCTGTGTGAGGGAGGGGTGTGTGGCC CAGGGAGGGCTGGCGGGCGGCCAGCAGCAGAGGCAGGCTCCCAGCTGTGCTGTCAGCTCACCCCTGCGCT CGCTCCCCTCCGGCACAGGCGCCTTCGGTCCAGTTGCCTTCTCCCTGGGGCTGCTCCTGGTGTTGCCTGC TGCCTTCCCTGCCCCAGTACCCCCAGGAGAAGATTCCAAAGATGTAGCCGCCCCACACAGACAGCCACTC ACCTCTTCAGAACGAATTGACAAACAAATTCGGTACATCCTCGACGGCATCTCAGCCCTGAGAAAGGAGG TGGGTAGGCTTGGCGATGGGGTTGAAGGGCCCGGTGCGCATGCGTTCCCCTTGCCCCTGCGTGTGGCCGG GGGCTGCCTGCATTAGGAGGTCTTTGCTGGGTTCTAGAGCACTGTAGATTTGAGGCCAACGGGGCCGACT AGACTGACTTCTGTATTTATCCTTTGCTGGTGTCAGGAAGTTCCTTTCCTTTCTGGAAAATGCAGAATGG GTCTGAAATCCATGCCCACCTTTGGCATGAGCTGAGGGTTATTGCTTCTCAGGGCTTCCTTTTCCCTTTC CAAAAAATTAGGTCTGTGAAGCTCCTTTTTGTCCCCCGGGCTTTGGAAGGACTAGAAAAGTGCCACCTGA AAGGCATGTTCAGCTTCTCAGAGCAGTTGCAGTACTTTTTGGTTATGTAAACTCAATGGCTAGGATTCCT CAAAGCCATTCCAGCTAAGATTCATACCTCAGAGCCCACCAAAGTGGCAAATCATAAATAGGTTAAAGCA TCTCCCCACTTTCAATGCAAGGTATTTTGGTCCTGTTTGGTAGAAAGAAAAGAACACAGGAGGGGAGATT GGGAGCCCACACTCGAATTCTGGTTCTGCCAAACCAGCCTTGTGATCTTGGGTAAATTCCCTACCACCTC TGGACTCCATCAGTAAAATTGGGCGTGGACTAGGTGATCTCATAGATCCTTCCTGCTGGAACATTCTATG GCTTGAATTATATTCTCCTAATTATTGTCAAAATTGCTGTTATTAAGTATCTACTGTGTGCCAGGCACTT TAAATAAATATTGTGTCTAATCTTCAAAACAAATTTGCAAGGAAGGTTTTTGGAGATAAGGAAACTGAGA CTCAGGATTAAGTAACACACCTAAAGTCACAGGTGAGCTTGGAACTGAACCCAAGTGTGCCCCCACTCCA CTGGAATTTGCTTGCCAGGATGCCAATGAGTTGTAGCTTCATTTTTCTTAGAGACTTTCCTGGCTGTGGT TGAACAATGAAAAGGCCCTCTAGTGGTGTTTGTTTTAGGGACACTTAGGTGATAACAATTCTGGTATTCT TTCCCAGACATGTAACAAGAGTAACATGTGTGAAAGCAGCAAAGAGGCACTGGCAGAAAACAACCTGAAC CTTCCAAAGATGGCTGAAAAAGATGGATGCTTCCAATCTGGATTCAATGAGGTACCAACTTGTCGCACTC ACTTTTCACTATTCCTTAGGCAAAACTTCTCCCTCTTGCATGCAGTGCCTGTATACATATAGATCCAGGC AGCAACAAAAAGTGGGTAAATGTAAAGAATGTTATGTAAATTTCATGAGGAGGCCAACTTCAAGCTTTTT TAAAGGCAGTTTATTCTTGGACAGGTATGGCCAGAGATGGTGCCACTGTGGTGAGATTTTAACAACTGTC AAATGTTTAAAACTCCCACAGGTTTAATTAGTTCATCCTGGGAAAGGTACTCTCAGGGCCTTTTCCCTCT CTGGCTGCCCCTGGCAGGGTCCAGGTCTGCCCTCCCTCCCTGCCCAGCTCATTCTCCACAGTGAGATAAC CTGCACTGTCTTCTGATTATTTTATAAAAGGAGGTTCCAGCCCAGCATTAACAAGGGCAAGAGTGCAGGA AGAACATCAAGGGGGACAATCAGAGAAGGATCCCCATTGCCACATTCTAGCATCTGTTGGGCTTTGGATA AAACTAATTACATGGGGCCTCTGATTGTCCAGTTATTTAAAATGGTGCTGTCCAATGTCCCAAAACATGC TGCCTAAGAGGTACTTGAAGTTCTCTAGAGGAGCAGAGGGAAAAGATGTCGAACTGTGGCAATTTTAACT TTTCAAATTGATTCTATCTCCTGGCGATAACCAATTTTCCCACCATCTTTCCTCTTAGGAGACTTGCCTG GTGAAAATCATCACTGGTCTTTTGGAGTTTGAGGTATACCTAGAGTACCTCCAGAACAGATTTGAGAGTA GTGAGGAACAAGCCAGAGCTGTGCAGATGAGTACAAAAGTCCTGATCCAGTTCCTGCAGAAAAAGGTGGG TGTGTCCTCATTCCCTCAACTTGGTGTGGGGGAAGACAGGCTCAAAGACAGTGTCCTGGACAACTCAGGG ATGCAATGCCACTTCCAAAAGAGAAGGCTACACGTAAACAAAAGAGTCTGAGAAATAGTTTCTGATTGTT ATTGTTAAATCTTTTTTTGTTTGTTTGGTTGGTTGGCTCTCTTCTGCAAAGGACATCAATAACTGTATTT TAAACTATATATTAACTGAGGTGGATTTTAACATCAATTTTTAATAGTGCAAGAGATTTAAAACCAAAGG CGGGGGGGCGGGCAGAAAAAAGTGCATCCAACTCCAGCCAGTGATCCACAGAAACAAAGACCAAGGAGCA CAAAATGATTTTAAGATTTTAGTCATTGCCAAGTGACATTCTTCTCACTGTGGTTGTTTCAATTCTTTTT CCTACCTTTTACCAGAGAGTTAGTTCAGAGAAATGGTCAGAGACTCAAGGGTGGAAAGAGGTACCAAAGG CTTTGGCCACCAGTAGCTGGCTATTCAGACAGCAGGGAGTAGACTTGCTGGCTAGCATGTGGAGGAGCCA AAGCTCAATAAGAAGGGGCCTAGAATGAAACCCTTGGTGCTGATCCTGCCTCTGCCATTTCTACTTAAGC CAGGGTTTCTCATATGTTAACATGCATGGGAATTCCCTGGGCATCTTCTTGTGGTGTGGAGTCTGACTTA GCAAGCCTCGGGTGGGTTTGAGGGTCAAATTTCTACCAGGCTTATATCCCTGGTGATGCTGCAGAATTCC AGGACCACACTTGGAGGTTTAAGGCCTTCCACAAGTTACTTATCCCATATGGTGGGTCTATGGAAAGGTG TTTCCCAGTCCTCTTTACACCACCGGATCAGTGGTCTTTCAACAGATCCTAAAGGGATGGTGAGAGGGAA ACTGGAGAAAAGTATCAGATTTAGAGGCCACTGAAGAACCCATATTAAAATGCCTTTAAGTATGGGCTCT TCATTCATATACTAAATATGAACTATGTGCCAGGCATTATTTCATATGACAGAATACAAACAAATAAGAT AGTGATGCTGGTCAGGCTTGGTGGCTCATGCCTGTATTCCCTAAACTTTGGGAGCCTAAGGTGAGAACTC CTTGAACTCCTAAGGCCAGGAGTTCAAGACCAGCCTGGATAACATAGCAAGACCCCATCTCTACAAAAAA CCAAAACCAAACAAACAAAAATGATAGTGGTGCTTCCCTCAGGATGCTTGTGGTCTAATGGGAGACAGAA CAGCAAAGGGATGATTAGAAGTTGGTTGCTGTGAGCCAGGCACAGTGCTGATATAATCCCAGCGCTATGG GAGGCTGAGGTGGGTGGATCATTTGAGGCCAGGAGTTTAAGACCAGCCTGGTCAACATGGTAAAACCCCA TCTCTACTTAAAAATACAAAAAAGTTAGCCAGGCATGGTGGCATACACCTGTAACCCAGCTACTCAGGAG GCTGAGGCACATGAATCACTTGAACCCAGGAGGCAGAGGTTGCTGTGCACCACTGCACTCCAGCCTGGGT GACAGAACGAGACCTTGACTCAAAAAAAAAAAAAAGAAGTTTGTTGCTATGGAAGGGTCCTACTCAGAGC AGGCACCCCAGTTAATCTCATTCACCCCACATTTCACATTTGAACATCATCCCATAGCCCAGAGCATCCC TCCACTGCAAAGGATTTATTCAACATTTAAACAATCCTTTTTACTTTCATTTTCCTTCAGGCAAAGAATC TAGATGCAATAACCACCCCTGACCCAACCACAAATGCCAGCCTGCTGACGAAGCTGCAGGCACAGAACCA GTGGCTGCAGGACATGACAACTCATCTCATTCTGCGCAGCTTTAAGGAGTTCCTGCAGTCCAGCCTGAGG GCTCTTCGGCAAATGTAGCATGGGCACCTCAGATTGTTGTTGTTAATGGGCATTCCTTCTTCTGGTCAGA AACCTGTCCACTGGGCACAGAACTTATGTTGTTCTCTATGGAGAACTAAAAGTATGAGCGTTAGGACACT ATTTTAATTATTTTTAATTTATTAATATTTAAATATGTGAAGCTGAGTTAATTTATGTAAGTCATATTTA TATTTTTAAGAAGTACCACTTGAAACATTTTATGTATTAGTTTTGAAATAATAATGGAAAGTGGCTATGC AGTTTGAATATCCTTTGTTTCAGAGCCAGATCATTTCTTGGAAAGTGTAGGCTTACCTCAAATAAATGGC TAACTTATACATATTTTTAAAGAAATATTTATATTGTATTTATATAATGTATAAATGGTTTTTATACCAA TAAATGGCATTTTAAAAAATTCAGCA >gi|10834984|ref|NP_000591.1| interleukin-6 precursor [Homo sapiens] MNSFSTSAFGPVAFSLGLLLVLPAAFPAPVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKE TCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQA RAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALR QM KatG Mycobacterium tuberculosis H37Rv VO_0012369 885638 15609045 Rv1908c AL123456 NP_216424 1SJ2 83332 2153888 2156110 - catalase-peroxidase 4.87 75544.06 740 Rv1908c, (MTCY180.10), len: 740 aa. katG, catalase-peroxidase-peroxynitritase T (EC 1.11.1.6) (see citations below), HPI. FASTA results: Q57215 CATALASE-PEROXIDASE from Mycobacterium tuberculosis (740 aa) opt: 5081, E(): 0, (100% identity in 740 aa overlap). Contains peroxidases active site signature (PS00436) and ATP/GTP-binding site motif A (P-loop; PS00017). Cosmid sequence was corrected to agree with a sequencing read from the H37Rv genome. DELETIONS OR DEFECTS IN KATG GENE CAUSE ISONIAZID (INH) RESISTANCE. BELONGS TO THE PEROXIDASE FAMILY. BACTERIAL PEROXIDASE/CATALASE SUBFAMILY. KATG TRANSCRIPTION SEEMS TO BE REGULATED BY FURA|Rv1909c PRODUCT. The catalase-peroxidase activity is associated with the amino-terminal domain but no definite function has been assigned to the carboxy-terminal domain. TBparse score is 0.923. >NC_000962.3:2153888-2156110 Mycobacterium tuberculosis H37Rv, complete genome ATCAGCGCACGTCGAACCTGTCGAGGTTCATCACCTTGTCCCAGGCAGCGACGAAGTCCTGCACGAACTT CGGCTGCGCGTCATCGGCGCCATAGACCTCGACAAGCGCCCGCAACTCCGAGTTGGACCCGAAGACCAGG TCCACGCGGCTGCCGGTCCACTTCACCTTGCCACTGCCATCCTTGCCCTGGTAGGTCCCGTCATCTGCTG GCGAGGGCTCCCAGGTGATACCCATGTCGAGCAGGTTCACGAAGAAGTCGTTGGTCAGTGACTCGGAGGC CTCGGTGAACACGCCCAGCGGTAAGCGCTTGTAGTTTGCGCCGAGGACGCGCAGGCCACCTACCAGCACC GTCATCTCAGGGGCACTGAGCGTAAGCAGGTTCGCCTTGTCGAGCAGCATGTACTCGGCCGGCAACGGGT TGCCCTTTCCGAGGTAGTTTCGGAAGCCATCTGCCTTGGGCTCCAGCACGGCAAAGGATTCCACGTCGGT TTGTTCCTGCGACGCATCCGTGCGGCCCGGGGTGAAGGGCACCGTGATGTTGTGGCCAGCCGCCTTTGCT GCTTTCTCTATGGCGGCACAGCCACCGAGCACGACGAGGTCGGCGAAGGACACTTTGATGTTCCCCGGCG CCGCGGAGTTGAATGACTCCTGGATCTCTTCCAGGGTGCGAATGACCTTGCGCAGATCCCCGTCGGGGTC GTTGACCTCCCACCCGACTTGTGGCTGCAGGCGGATGCGACCACCGTTGGCGCCGCCGCGCTTGTCGCTA CCACGGAACGACGACGCCGCCGCCCATGCGGTCGAAACTAGCTGTGAGACAGTCAATCCCGATGCCCGGA TCTGGCTCTTAAGGCTGGCAATCTCGGCTTCGCCGACGAGGTCGTGGCTGACCGCAGGGACCGGATCCTG CCACAGCAGGGTCTGCTTGGGGACCAGCGGCCCAAGGTATCTCGCAACGGGACCCATGTCTCGGTGGATC AGCTTGTACCAGGCCTTGGCGAACTCGTCGGCCAATTCCTCGGGGTGTTCCAGCCAGCGACGCGTGATCC GCTCATAGATCGGATCCACCCGCAGCGAGAGGTCAGTGGCCAGCATCGTCGGGGAGCGCCCTGGCCCGCC GAACGGGTCCGGGATGGTGCCGGCACCGGCGCCGTCCTTGGCGGTGTATTGCCAAGCGCCAGCAGGGCTC TTCGTCAGCTCCCACTCGTAGCCGTACAGGATCTCGAGGAAACTGTTGTCCCATTTCGTCGGGGTGTTCG TCCATACGACCTCGATGCCGCTGGTGATCGCGTCCTTACCGGTTCCGGTGCCATACGAGCTCTTCCAGCC CAAGCCCATCTGCTCCAGCGGAGCAGCCTCGGGTTCGGGGCCGACCAGATCGGCCGGGCCGGCGCCATGG GTCTTACCGAAAGTGTGACCGCCGACGATCAGCGCCGCTGTTTCGACGTCGTTCATGGCCATGCGCCGAA ACGTCTCGCGAATGTCGACCGCCGCGGCCATGGGGTCCGGGTTGCCGTTCGGCCCCTCCGGGTTCACGTA GATCAGCCCCATCTGCACCGCGGCCAGCGGGTTCTCCAGATCCCGCTTACCGCTGTAACGCTCATCGCCG AGCCAGGTGGCTTCCTTGCCCCAATAGACCTCATCGGGCTCCCACTGGTCGACCCGGCCGAAGCCGAACC CGAACGTCTTGAAGCCCATCGATTCCAGCGCGCAGTTGCCGGCGAAAACAATCAGGTCCGCCCATGAGAG CTTCTTGCCGTACTTCTTCTTGACCGGCCACAGCAGCCGGCGCGCCTTGTCCAAGCTGGCGTTGTCGGGC CAGCTGTTAAGCGGCGCGAACCGCTGCATGCCGCCCCCGGCGCCGCCGCGGCCGTCGTGGATGCGGTAGG TGCCGGCAGCGTGCCACGCCATCCGGATAAACAGCGGCCCGTAGTGGCCGTAGTCGGCGGGCCACCACGG CTGCGAGGTGGTCATCACTTCCTCGATGTCCCGCGTCAGGGCGTCAACGTCGATGGTCGCGACCTCCGCG GCATAGTCGAACGCCGCACCCATCGGGTCAGCGACGGCCGGGTTTTGGTGCAGTACCTTCAGATTGAGCC GGTTGGGCCACCAGTCCTGGTTTCCGCCGCCCTCGACGGGGTATTTCATATGACCCACGACGGGACAGCC GTTGCTAGCGGCTCCGGTGGTGGTTTCTGTAATGGGTGGGTGTTGCTCGGGCA >NP_216424.1 catalase-peroxidase [Mycobacterium tuberculosis H37Rv] MPEQHPPITETTTGAASNGCPVVGHMKYPVEGGGNQDWWPNRLNLKVLHQNPAVADPMGAAFDYAAEVAT IDVDALTRDIEEVMTTSQPWWPADYGHYGPLFIRMAWHAAGTYRIHDGRGGAGGGMQRFAPLNSWPDNAS LDKARRLLWPVKKKYGKKLSWADLIVFAGNCALESMGFKTFGFGFGRVDQWEPDEVYWGKEATWLGDERY SGKRDLENPLAAVQMGLIYVNPEGPNGNPDPMAAAVDIRETFRRMAMNDVETAALIVGGHTFGKTHGAGP ADLVGPEPEAAPLEQMGLGWKSSYGTGTGKDAITSGIEVVWTNTPTKWDNSFLEILYGYEWELTKSPAGA WQYTAKDGAGAGTIPDPFGGPGRSPTMLATDLSLRVDPIYERITRRWLEHPEELADEFAKAWYKLIHRDM GPVARYLGPLVPKQTLLWQDPVPAVSHDLVGEAEIASLKSQIRASGLTVSQLVSTAWAAASSFRGSDKRG GANGGRIRLQPQVGWEVNDPDGDLRKVIRTLEEIQESFNSAAPGNIKVSFADLVVLGGCAAIEKAAKAAG HNITVPFTPGRTDASQEQTDVESFAVLEPKADGFRNYLGKGNPLPAEYMLLDKANLLTLSAPEMTVLVGG LRVLGANYKRLPLGVFTEASESLTNDFFVNLLDMGITWEPSPADDGTYQGKDGSGKVKWTGSRVDLVFGS NSELRALVEVYGADDAQPKFVQDFVAAWDKVMNLDRFDVR Protective antigen DNA vaccines encoding KatG antigen fused to TPA signal sequences evoked a significant protective response in mice aerogenically challenged with low doses of Mycobacterium tuberculosis Erdman 17 to 21 days after the final immunization [Ref1480:Li et al., 1999]. leuD Mycobacterium tuberculosis H37Rv 888225 15610124 Rv2987c BX842581 NP_217503 3H5J 83332 3344032 3344628 - isopropylmalate isomerase small subunit 4.89 20390.6 198 catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate in leucine biosynthesis; forms a heterodimer of LeuC/D >gi|57116681:3344032-3344628 Mycobacterium tuberculosis H37Rv, complete genome CTCAGGGGGCGGGTAGAGTGCGCGGTTTCCAGTACGCGCACGCACCCTCAAAGGCCTCGATCTCGTCGAG TTTCCGCAGCGTAAGGGCTATATCGTCGAGACCTTCAAGCAGCCGCCACGCCGAGTGGTCGTCAATCTTG AACGGCAGCACCACTGTTGCTGCGGTGATAATTCGATCTTGAAGATTGGCAGTGATTTCCAGGCCCGGAC TCTGCTCAATGAGCTTCCACAGGAGTTCCACATCGTCTTGGGCAACCTCGGCCGCCAGCAGCCCGGCCTT GCCCGCGTTGCCGCGGAAAATGTCACCAAATCGGGATGAGATAACCACCCGGAATCCGTAGTCCATGAGC GCCCAGACCGCATGCTCTCGCGAGGATCCGGTGCCGAAATCGGGCCCGGCAACCAGGACCGAACCCCGGT CAAAGGGACTGAGGTTTAGCACGAATGCAGGATCCGACCGCCAACCCGCGAACAAGCCGTCCTCGAAACC GGTTCGGGTGACCCGCTTCAGAAAGACCGCGGGAATGATCTGATCGGTGTCGACATTGGACCGCCGCAAC GGCACGCCAATACCAGAGTGGGTGTGAAAGGCTTCCA >gi|15610124|ref|NP_217503.1| isopropylmalate isomerase small subunit [Mycobacterium tuberculosis H37Rv] MEAFHTHSGIGVPLRRSNVDTDQIIPAVFLKRVTRTGFEDGLFAGWRSDPAFVLNLSPFDRGSVLVAGPD FGTGSSREHAVWALMDYGFRVVISSRFGDIFRGNAGKAGLLAAEVAQDDVELLWKLIEQSPGLEITANLQ DRIITAATVVLPFKIDDHSAWRLLEGLDDIALTLRKLDEIEAFEGACAYWKPRTLPAP Virmugen Deletion of leuD gene was shown to be highly attenuated. Immunocompetent BALB/c mice vaccinated with the DleuD auxotroph were protected as well as BCG in terms of survival following an intravenous or aerosol challenge with virulent M. tuberculosis [Ref1808:Hondalus et al., 2000]. Ltf Mus musculus 17002 31560677 AC118727 NP_032548 10090 9 111019291 111042765 + lactotransferrin 8.61 73470.01 707 Also known as Lf; Csp82; Ms10r; MMS10R >gi|372099101:111019291-111042765 Mus musculus strain C57BL/6J chromosome 9, GRCm38 C57BL/6J CAGGCTTGTCCTCTAGGTCTCCCAAGACCACAGACATGAGGCTGCTCATCCCTTCCTTGATATTTCTTGA GGCCCTTGGTAAGTGCAGGTGCCCAGAGGTAGGGAGGGGCGGGAGGTCACCTGCTGGGAAGCTGGGCACT AAATGCACTCTTCTGTTATCTAGGGAAGACTGTTGGGCTCAGAGAGGGTGAGATGTTCCTGTGAGTCCCA GTTCACTGCCTCCCAAGAAGGCTTCTTTGTACCAACATTGGGGACCCTCCATGTTTGACTCTATGCCCTC TCTTAGTCCTCCTGCTGCTGGACTCCACATCTAACCCAGGCATCCTATACAGGCAGGCTAGCTCAGTGGC CAGTGGTGCTGGTGCCCAACCGCCTAGGTTCAAATTCTAGAGTCAACACAACTTAGCTGGAAAGTCATAT AACTTGGTAAAGCTACACAATGCCTAGAACTATGGGGGTATTGACAAAAATCACACTGAAGGATAGATGG GGTGACCCAGTGAGTAAAGGTGCTTGCTACCAAGACTGAAGACAGAAGTCCTAGTCCCAGAACTCACTCA ATGGAACTACCAGCTTTCTATCTATAAATTTATCTTTTCTGGTCGAGTGTGGTGGTGCACACCTTTGATT CTAGCACTTGGGAAGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGC AGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAGGCAGAG GCAGAGGCAGGCAGATCTCTGTGAGTTCAAGGTCAGCCTGCTCTACATAGTGAGTTCCAGGATAGCCAAG GCTACGTAGAGACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTGGAGTTATCCAATATTTTGTCTT GGATTTGGCTTCCTTCATCTAGTCATGTTATATGTATGTGTATTATTTTTTATATAATTGAAGAATATTC CATTGAATGGATGTACTGCATTTTCTTCAGCCATTCCTATTCACCAGTGTTTGGGATTTATGTAGTTAGT TGGGTTTTTTTGTTGTTGTTGTTATTTTGAGACCTTTTTCCTATCTAAATAATGCTACTATGAATATTAA TGCTAGATCTTTAAGCAGATTTATGTTTTTATTTCTATGAGCAATTGCCAACCTGTTTCCCAAAGTGGCT ACATCATTTTACAGTCTCAGCAATGTAAGAAGGTTTCCATGTTTGCAACATTAAGATCCAGTGTGCACAT GAGTAAAACATGAGCCACGCCATTTCCCTGCTTCCCTGCCAGGTTCATCTAGGACAAGGCTGATGGAGGT CAATAAAATGATCTTTGGAGTGGATCATCTTTGGGGGTGGGGTGGGTGTCTGAGTCTCATCCCTCTGTGG TCCAGCAGCTTAGCTTAACTAGAGTACAGTGGCTGAACAAGACCACGTAACACGGAGGGTGGGGCAGTGA CCACGGGCAGTCTACAGAATGCCCTCAGACATCGGGGAGGTCTTCCCCAACGAGCACTGCTGGATTCCAT TCTGTCTTTCTCCCAGGACTCTGTCTAGCTAAGGCAACAACTGTTCAATGGTGTGCTGTGTCAAATTCTG AGGAAGAAAAATGTTTAAGGTGGCAGAACGAGATGAGAAAAGTGGGTGGCCCGCCGCTCAGTTGTGTCAA GAAATCCTCCACCCGCCAGTGCATCCAGGCCATTGTGGTAAGCTAACACTCCAACCCGAGTGGAGGAAAG CAAGGAGAGGTGCGCAGTCCAACCACAAGTTCGAGTCTCTGGTCCCTCTGCTTCACTTCCTGGGCACCAG TCAGTTGAGAGCAGTCTTTGCTCACAGAAACCGGTGTCTCTTGATATCTAAAGAAAGACAAATGACAGGC CTCTTATGGGGTAACTGGGGTCTTATTTCAAATTGTTCATAGGAACTATTTTTTTAAAAGCCTAAGCAGC CATATTTGATGAATATGTAATGTCTCTAATCCCTTGAGAAATAAGCCTGGTGCAGCCCTAGATGCCATAT CCCAACTGTTGGGTAGGGATGGCTAGGACTCCAGACATTAAAAAGACACTTGTTTGCCCTCCAGCACCTA TGTTAAAACCTAGAGATAGTAGCTTTCCCACTTGGACTCTGGGTCTAGGAAGTAGAGACTGGAGGTCTAA ACAAACTGGTGAGCTCCAGGTTTGGCAAAAGACTTTGTCTCAAAACGTAAGTCAGAAAGAGATGGAGGAA GACACCAGAAGTCGAGCTCTGGCCTCCACACGTTCATGTCCTCCAGTGTGTGAGCACACACACACACACA CACACACACACACTGCGCACATCTACAAGACTGAAAATACACATGAAGGCAAAATAGGTGATAAAGTATG TGGATCAAAAAGCTTTAAATACAGAAGATTGTGACAGTGTGACCAAGAGTAACTGAAGTGGACAGGCATA CGTAGGGGTCACACACAACTTCAGGAGTTAAAGCCCTGGGGGTGCTTACTGCTCACATCGAATGACCCCT GACCTGTGCTGTTGTCATCTTCTAGGTAGGACATATGGACAATACGAAGGGCTAACTCATTCTGAAGTCC TGAAATCTTTTCTCCTGAAGCATGTCTGGACCCATTTTCTTTCAGACAAACAGAGCTGATGCCATGACTC TTGATGGTGGCACTATGTTCGATGCAGGAAAGCCCCCCTACAAACTGCGACCTGTGGCAGCTGAAGTCTA CGGGACCAAAGAGCGTGAGTTCTTTTTCTGTGGACGCTGAAACCTCGTGACTGTGTTGTGGCTATGAAAT GGTTTCTGTGGCGTGGGAGTGGACGAGTAAACCTAGTCAGTGCCTCTCACTGGGGAACAGAGTCTCTGGA CTCTGGATGACAGGTCCTCCTGGTGCTTGGGTCTCCTTGCCATGGCAGCTTGGGCAGAGTGGATTCTTCA CCTCCCTCTCATCGGGGCTAAGCCTGTCTAGTGCCTCTGTTCCTCTGCAGAGCCCCGGACTCACTACTAT GCGGTAGCAGTCGTGAAGAACAGCAGTAACTTTCATCTGAACCAACTCCAAGGCCTGAGGTCCTGCCACA CCGGCATTGGCAGGAGTGCGGGGTGGAAAATCCCTATAGGGACACTTCGTCCATACCTGAATTGGAATGG GCCACCTGCATCCCTTGAGGAAGGTAAAATGGCTTGTGGGGGAGCGGGGAGAGATCCTGAGTGGTCCAAG CTGGGAATTTCTAGTCTCACTGTAGACACAAGCCACTTTGGGATGGCTTTCCATATGGGATGAGTCCAAG GTTGGGCAGAGGGAGTCTAATCAAGTAGAAGCAGGGGCCATCACACCAACAACTCCGGGAGCCGCCAGTA GCTGTTCTCTGGGCAAAGATGCACGTCTGAGTTCCGAAGCCCACTCAGCTCTGTCCTGTTCTGCCTGGCG GAGGAGGGGGCTCTTGGTCCGTGGAGGGTGGAGGGGTGGCTGCTCCTGGGAGACAGGACTGCAGTGGCTG GGATCGGCTCATCCTCTGTGGCCCACTTCTCTGTGTTTGACAGCGGTATCCAAGTTCTTCTCAAAGAGCT GTGTTCCCGGTGCCCAAAAGGATAGATTCCCCAACCTGTGTAGCTCGTGTGCAGGGACAGGAGCCAACAA ATGTGCCTCTTCCCCCGAGGAGCCATACTCAGGTTATGCTGGAGCCTTGAGGTGAGCGACACCATTCTCT CCTCCCCCACGGCCCATGGTCGCACAGCTTCAGGCCAAGGTGCGGGATGGAGATTTGGTCCTACTGATGT GCAGACGGAGAACCCAAGCCTAAGAAGTGGTTGGTCTCTCTACTACATGTCCTTCTCCAGCCCCTTAGAG GCCCAACAAGGCCCCAAAGTCTATATCACTTTGATGGAAGCTAACCTGGTTATGGCCCCTCTGATCACCT CACGCCTGATTTTGCTCTCCTATTTTTTGGGGTTATCCTGCTAACTTTCTCCCTGTTTTCATCCCATCTG GGTGGGGCAGCCATTGTGGCCTTGATATTTAGTCCAACTCAGGCCTCTCTCTCGAGTAGGAGAGGGTGAC CTCTGTCTGACTTCCTCCTCTGGCCTCTATGCTGTTGCCTAGGCTCAGCACCTCCTCTCTAGTCCTTTCC TGAAGCTCAGGCACTTTTGTAGAGTGACTTCCCTAGGTTCTTGCTGGCCCTAGCACATTTGCCCACTCAG CATGGAGAGAAAGAGCCCCATGGTCGGGGACACCTCATCTTTAACTGCGTTCTTCTCATTTCACAGGTGT CTGAGAGACAATGCTGGAGATGTGGCTTTTACCAGAGGAAGCACGGTATTTGGTAAGGGCAAGGGTCAGG CTGTGGGTGTGGCCACCTCTTAAGTGCTTTATCTGTGATGTGCGGGTCACCAGATTCCCCAGTTGTGATC AAGTTGCCTACTGGGTCCAACTCTTCTTTTAGGTTTTTCTTTTAAAAAAATACTTTAAGATTAATTATGA TTTTACGTGCATGGGTGTTTTGCCTTCGTGTATGTCTGTGCACCACATGTGTGCCTGATACTCAAGGAGA CCAGAAGAGGGTGTCAGATCCCTTGGGACTGGAGCTACAGGTGGTTGTGAGCTGCTATGTGGGTGCTGGG AAGTGAACCCAGGTTTTCTGTGAGAGTAACCACTGAGCAACCTCTCCAGCCCCTACCTCATGCTCTGAAA TCTCCCACTTCTACCCCCTTCTCCTTCCCCTCACAGACACTCTCACCAGGGAGTTCGTCATGTGCTTGCC CAGGTGTACACGCCTCTGCACCATAAACTTCACTGGCTTGTGTCTCTGGATTCTGAGTTCACACAGCTTT TACTGAACACTAGAAGCAGCGCGAGCCCAGGGCTTTCCACTCAGCATTGGGGGTCCAAGATTGATCCGTG GTTTTACTACACGAGTAACTCCGTGGTTCTGACAGTAGAAGAACGATCCGTGGAACATGTCTACCATATT TAATTAACGTCTCTGTCAGTCTAATGACAGACAGACAGGCGCGGCGGTCCTCCCTCCTTTCGGCTTTACC TTTTTGAGTGCTCAGGAAGAGTGCTCATCTTCACACGTTTCCCTTCCTCCCAGAGGAGTTACCAAATAAA GCCGAAAGGGACCAGTACAAGCTGCTCTGCCCAGACAACACCTGGAAGCCGGTGACAGAATACAAGGAGT GCCACCTGGCCCAAGTCCCTTCACATGCTGTCGTATCCCGAAGCACGAATGACAAAGAAGAGGCCATCTG GGAGCTTCTCCGCCAGTCACAGGTACAGCTACAAGTGCACAGCACCCCTTGCCTGCTTAGGTTTAGGGGT GGGGAGGGCCTCTCTTTCCCTAACTTCAGCAGTTGAGAGAGTTCCATAGTTTAAGGACCATGGGCCCTGA CTCTCCCTTGGACAGTAGAGACATAGGAACCTTCTTCAGTGTCAGCCCCTAGCAGGCTTTCCTGGGCTTC CGCAGAGGCAGGGGTGGGTACCGGATGCTATTCTTAGGTCCCCTAAGGACCTTGGGACAAAACAGTTTTC TGATGAGGTAAGTTATCGTTGAGCTCATGTCTCTGCAATGAGCAGAGTCCCTGGGAGCTCGGATATCAAT TCCATTTGAATTGTCCCCAGATCCCAAGTAGCTGTTTCCCACACTGGGTTTCGATTCCTTCCTGCCTACT GCTGGTGACCATAATTCTATTTTCTTTCCTTAAACAGGAGAAGTTTGGAAAAAAACAAGCATCGGGATTC CAGCTCTTTGCCTCCCCCTCGGGACAGAAGGACCTGCTGTTCAAGGAGTCTGCCATTGGCTTTGTGAGGG TTCCCCAGAAGGTAGATGTAGGGCTCTACCTGACCTTCAGCTACACCACATCCATACAGAACCTGAATAA AAGTAAGTGGACCCCAGGCAGGAACCCAAGGGTACTGAGCCAGCAGTACCTCTGGGTGGGTGACCTCTGC TCTGGGAGGGTCTCTCCGCTACTGGTGGGGCTGACGCACATACTGTGTGTCAAGTAAGTCAAGGCCATTG TGAAAAATGTCTCCAAAAAAAGTCCCAGACGCTGGACTAATGCTTCCATATTCCTCTGTGTATGCCAGGA TCCCAAGGCCTCTTATTAAGTGAGAAAGAGAGGCATGGACTAAGAAAAGAAGAATCTGGGGAGATTACTC CAGAGGCTAGGTGAAGCCCCCCTTCTGTGCTGGGATTCGAACTTGGCTGAAGAGATTACAATGGGCTGTA GAGATGACAACTTTGGCTATGTTTATCCTACTTGGGAGGACTTAGAGAGGGAGTCTGAGTGAACCAGGAG ACTGTCCTGTGACATCTGCTGGTGACAGAGCCTTATGGGTGGGTGGTCGGGCTCAGTCAAGGTCCTCTAA GCCAGCCTTTGGGGTATACACTAGTTTCCATGTCATGAAGCTTTTATTCTGAGGGACAGAACATTAGGCA CTCCACTCATTACACCAAGGACTGATGGATGAATTAGAAGCCAGGGAAGCCCTCTGAAAGAGCAGAAGGA GAAACAGAAAGCTTCCGGCCAAATCCAGTTTGAGGGCTGTGATGCGGGGAAAGAGAAGGTCTCACACAGA TTCCTCGGTCTCAGAAGATCTGGGCAGGACCTTGATCCCCAAGGAGGGCAGAGGTGGGAGTGAGGCAGCG CCTGAGTGTTCCTCACCACTGTCCTTCCCCACAGAGCAGCAGGATGTGATAGCCTCAAAGGCCCGGGTCA CATGGTGTGCCGTGGGCAGTGAGGAGAAGCGCAAGTGTGATCAGTGGAACAGAGCAAGCAGAGGCAGGGT CACCTGCATCTCATTCCCCACCACGGAAGACTGCATTGTCGCAATCATGGTAGGGAGCCCGGGTCACACG TGCTGTGGGTGGTGGGCTTCTTAGAGGGCACGGACCATGGTCATCTACTTATAGTGAGGTTGGTCACCCG AGAGGCCACTGTGAAGAAGGAACACCCAAAAGAGAAGGTGGGCTCGGGACCACTCATGCACATGTGTTGC ATGAAGGAGCGGCCTTCTAGGTTTGAAAACCAATTTGTTGTGGAATTTCTGCCCTTTCCAGTAAAAGTAC AATTTAGGGTTATTAGGTTCATTTGGAAAATATGGAACAATTTTAGCATCTCTCTCTCTCTCTCTCTCTC TCTCTCTCTCTCTCACACACACACACACACACACACACACACACACACACACACACCTCTGCATGCAACT TCATATAGAAATATTTACAGTGAGAGTTTTGAAATTCCCCACCCCAGGTTTCTCTGTCCATGTAGGCCGA GCAATTCTGGATTTGTATGGGTTATTCTCTTTTGATCCCTCGTTCAAATAAGCAGTATACCATGGCTACT TCCCCACATCAATGCTAATCTCGGGAAACAGTTTTAAATGCTTATTTTATTTTATGTATATGTGTGAGTG TCTGAACGTGTATGGGAGCCACATGCATGCAGGTGCCAGAAGAGGGTGTTGGATCCTCCAGAAGTAGCGT TAGAGACAGTTGGAGCTGCCATGTTGGTGCTGGAACTGAATTCAGGTTCCCCGAAAGAGCAATGGACGCT CTTAGCCACTGAACCAAATCTCTTGTCCCCTCTCATCATTTTTAACGCCCTTGTATAGCAGTGCTGACTG GGTAAAGGAGCGTGCAGTCCTGATAAACTCGTGTTTCTGCGAGGGTTGTGGCTTGTTCATTCTCATGAGT CAACTTGATACCCAATCTCTAGGTACCACGTTGAGTATCGTGCTAAGAAGCAGAAGTTAGCTGAAAGCAG CTTTTCTCAGCGTGTTTTCTGTGAGTGAGGCTGGTTCAGAAAGGAGTGCCAGGAGTGCTCGGGTACAGGA AGAGTTCTGTTCAGAAGACAGAGTTAGTGTCTCAGGTGTGTGGGTGACAGCCCTAAGATAGTGAGACCGG CGGAGGACTGAGTCCATGTGGTATTTCCTGGGGGTGTTACGTGCAAACAACACGTGTCTTGAGGCCATCC CTGAGAGCTCCGAAGACAGCATCTTTGTGTGGTGTGTGTGACTGGCTCTGTCTCTGCAGAAGGGAGATGC TGATGCCATGAGCCTGGATGGAGGCTATATCTACACTGCGGGCAAGTGCGGTTTAGTTCCAGTCTTGGCA GAGAACCAGAGTAAGTGGAGTTGGTGACCACAGGCTATGCCATATGAAGTTGACTTGGGAGTAGAGAGGT GTTACCTCACTCACTAGATCTTGGAGAAGATGGAGACAGAATCAGAAGAAGGAATAAATGAAAGGGGCCA TGACACCCACTTCACCCCATTCCCTTAGATTGACAGATCATGATAGTTTACTTCTTGATTAAAACAAAAA CAAAGACATCTTTGGGAATGGTGAGATGGCTCAGCTGGTACAGGTGACAACGGCTGCCAAGCCTGTTGAC CTAAGTTTGATGCCTAAGACCCACATGGTGGAAGGAACCAACTCCCACAAACTGTATGCAGCCCTCTATG CATGCACACACAGGTCATGTGCACCTGACATTATGTATATATAACATATTATTATAAATATGTATTTATT AACATATATGTTGCACACACAGAGCATGCATGTGCTGACAACAGGTGCATATTGACAACATGCATGTGCT ACTAGCATGAATACACTGATAGCATAAATGTACTGGCACCCAGATGAAGGGTCAGCCATCCCCAGAAGTC ACCCTGTGCCCTGCTGTCACCATACTTCCAAAAGGCAGCGACTCTCCTGACTTCCAAAGACATAGATCAC CTTGCCTCTCCTTTAAGTTAATGGAAAAAGAATTGAGCATTTGATGTCTGCTGTGCCTTGCTCAGCATTA TTTTTGTGACATCCACCCACACAAACGTGTGGCTGCTGATTAGCCAGTCTCGTGGCTGCATAACGTTCTA TTGTGTGACTGGGTGGCCGTGCTTCTCCATTGTCTTCATGGATGGGTGTTTCAGTGATTTCCATCTGGTG GTTATTGTGAAAGATACGGCTACAGCCATCCACGACCACGTCTATGATGAAAATGTGTATTTATTTCTGC TGCATTGATATACCGAGGCGTTCCCTTTGAGTTTGATCTTATCGTATTGTGGGCATTTGAAAGTTTATGG CCTCTCAGGAAATCTGAGTTGTCAGTAACGCTTTGCTTGTCTTTGCCCCTTCAGAATCCTCCAAAAGCAA TGGCTTGGATTGTGTGAACAGACCAGTGGAAGGTAAGTCGGGGGCAAAAATAAGTCACCACTCGAGAGGA GGCAGGGGGAAGGTCACTAACCACACAGAAGCCAAGCCTGCTGTTATTACAGATACAAATGGTTGATGGA GGAATAATTACAACTAAAGTCTGAATGCATCCTCATGCTTGCTGGGGAACTTTCATGTGGTCACTCTTAA TGCAAGCTTCTGTGGAAAGAGAAATGTTGCTCCCCCATTCTGTACATTCGAGGGAACAGTGGCCCAGAGA GTCCCAGTGTCTCCGATCTCACTCATACTGGCACTACGCTGCTGCAGATGCTGGATTGGAACCAGGGCTT CTCGCCTCCGCAAGCCCACGGCTCTGCACTAGCCTCAGCTGCTCTCCCGAGTTGGAGCTTGCTGAGCTGT GGAAGCCTTGGTACTTGCCCTCAGTTGGGTTATCAGATGCCTTCGGAGGGGTTCAGCAACCATGTTAACT CCCTCACTGTGCTGGGCTGGTTGCACTCAGCTCTTGGCGGTCCTTGGGTGCATGCCCTTAGCATTCATGA AGCAGATCTACTGGCCACCACAGCACTTACCTGTGTGTGTGATTTGTGCATTGCTGAGACCAATCTCATT GTTCAGAGTCTGTTGCCACTGGGTAAAGGTTTATAACTCAGCAATACTATGAGGCTGGTTGAGGACAGTT GCCTGTAACCACTTAGAGGACCAATTTTTCATATTATTTTATTTAACAATACAGGTTGGTCTTCCCTTGA CACAGAACACCTTTTTAAAATTTTCTTTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTCCTTC CTTCCTTCCTTCCTTCCTTTCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCTCCCT TCCTTCCTTCCTTCCTTCCTTCTTTCCTTCCTTTTCCTAACAATGGCCAATGATCCATGGTCATGTAATC CTGTTGTTTTTCCGGCCTGGGTTGGAGACACTGTACTTGAATCCATGTGCACCCTAGAAGCTAAGATGTT CTCTCTGTCATCCGTTGCACAAGGGTACCTTGCTGTAGCAGCAGTTAGAAGAGAAGATGCTGGCTTCACC TGGAGCTCTTTGAGAGGCAAGAAGTCCTGCCACACTGCCGTGGACAGGACCGCAGGCTGGAACATCCCCA TGGGCCTGCTTGCTAACCAGACCAGATCCTGCAAATTTAGTAAGCATATGGGAGGTTCTCACGGGGTGGT CATCCTGGGTCTGGGAGTGAGTACTTGAGGCTGTGGAACCTTAGGGGAGGGAGAGGGCAAGACGGTTGCT GACTGGCTGTCGCTTGTGGGCCTCTTTTAGAATAATTAGGTTCTCCTTCTGGTCGCCTCATGGAACCATG GGTGCACAGGATGTTTCACACAGAACACTTCCTAAGCTTCCCCAATTCCACCCCACCCACTGGGAGATCC AGATCGCAATAGCATTTAATTAAAACATTAAGATATGCTCAACCTTATGAGTTAGCAGTTAGCTGAATTG TTAACAATCAGATGTTTAGGTTTTTTGTTTTGTTTTGTTTTGGTTTGGTTTGTTTTTTTCTCATCGTAAA TCTAGTAAGATAGCGTGAATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTATATG TAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGAGGGAGTCAGATCTCATCAGATCTCATTACAGAT GGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACCTTCGGAAGAGCAGTCAGGTGCTCTTA CCCACTGAGCCATCTCACCAGCCCTAGCTTGAATTTCTAATGTAGGCAAGAATGCAGTAAAATGGGCATT TTCAAATGTTTTCATGTGTATAAAAAACTGATACCTGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACT GAACTGCTCTTCCAGAGGTTCTGAGTTCAATTCCCAGCAACCACATGGTGGTGGCTCATGACTATCTGAT GGAATCTAATATCCTCTTTGGTATGTCTGAAGAGAGTGACAGTGTACTCACATACATAAAATAAATCTTT TTTAAAAAAAAAAAAACAAACCCTGAGGCCTTTTAAGAATGTTTCCAGAGAGTTGGCACCCAGCACTCCT GAACACAAATCATTAGACCTACCTAGTACCATGGGTGTACCAGTCACAGGAACATAACAGCCATCAGGCA CCTTAATTGAGAAGCCTGGGTTTCTATTTCTGATTTTAAAAAATAAAAAGAAGTCAAGTTGTGGAGCAAG AGGCCTATCTGTGCAGGTGTGGCCTTCTCAGACACCAGCCCTGATATCCTATCCATTTGTACAGTCTGAA GCCTTTCTGATTGCCAGTGGGTCTTTGGTTGGTGGTAGAATGTCATTCTGGGGTGACAGGTATCCTGACT GTGCTGTCTACGTACTGAGCTGACATCATGCTATGCCATCTGCTGGGTAAAGACTTTGTGTCGACTGTGG TCCGAAGCACTAACTTATTGTCTTTCCTCTGTCTAGATGAGTTCTTTAGCCAAAGCTGTGCCCCTGGTGC TGACCCCAAATCCAATCTCTGTGCCCTGTGTATTGGTGATGAGAAGGGTGAGAACAAGTGTGCTCCCAAC AGCAAAGAGAGATACCAAGGCTACACTGGGGCTTTAAGGTAAGTCTGGGGCAGAGTGCCACCAGCCCAGG GCCTCAGCCCCCATTCTCAACAAGCGGCACTGTAGTTGGGGTGGGGTGCTCTCATAGAAGAGTCAAGGAT CAATGGGGGCAGAAGAAGCAAGAGCCTCCTATCATACCCAGCACCCCAGAGAGTAGAGGTTGGACAGCGG GTCATGGGGCTTACAGGGCTGCAAGGCTCAGCTTTGTCACCCTCAAAGTCAACGGGTAGAGAGTGTTTCA GTGCATTTCAGAGGATTCTAGTGACCACCACCCTTCCTTCTCCCTCACTCAGATCACCATTATCCTATGT TTTTAGTTTTCTCCTTGATTTTGGCCTTGAAGGGGCTGGAGAGATGATTCAGCCGCTGAGTGCTTGCTGT TTCATACAGAACTGCGTTCAGTTTCCAGGATCCCAATCAGGTCCAGGGGATTCAGTGTCCTCCTCTTAGG TCCAGAGTACCCACATGTGCACATATGTACATACAGGTGCACACATAAATAATAATAGGTCTTTTTAAAA AGAAGTGGCAAAGAGAAAGCTACATTACCACACCTTCTCAGTGCTGCATGGGTCACAATGACTGTACAGC TCACATTTACGTGGGTGTTGATTCTCACGGCCCTGTGTAGAACATGAGGCTCACGCAGAGGCTCCTTCCT CCATCCTTCCTTTAACACTAGATGTTGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT TCCAAAGGTCCTGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCTGTAATGAGATCTGATG CCCTCTTCTGGGGTGTCTGAAGACAGCTACAGTGTACTTACATATAATAAATAAACAAGTTAAAAAAAAA CACTAGATGTTGTCACAGACCCTCAGATGACAATATTCAAACGGGACTTTGGGCTTTTAAATATAAATTC AATAATTTTGCTCCCGTGAAACAATGACTTTTTTTTGGCTATATCTAAATGATTTGTAAACACTGATTTT TTTACGTGTATGAAACATGTCCTTAAGGACTCACATAGCCGGGCATTGGTGGCGCACGCCTTTAATCCCA GCACTAGGGAGGCAGAGGCAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTACAAAGTGAGTTTCAGG ACAGCCAGGGCTACACAGAGAAACCCTGTCTCAAAACAAACAAACAAACAAACAAACAAACAAACAAAAA GATTTACATATAGAAAGCCTACAGCATGATAGCATGATTCACTCTTATTGATTGATTGATTCACTCTTTA AATAGATAAGTTTGATAAGAAGAACCCAAGGGAATCCTGGGATCTGTTCCCCCAGACTTGCAGCCTGGTA TTTTCTCTCTGGAAACATCTGCACACCCTTACTCCTGTATACACTAAAAGTTTTTCTTCTGCTCAATTAG ATCTAGAATCTAGGCTGGGGACTATACAACAAAGTAATCCAAAGCTGGAGCCAGGACTCTTGCTTTTCTC TTACATCTTTATCTAAAACTCATTTAGGTTAGCATATAGCATGCTGGGTTTCATCATGGTGTCTCTAAAT TCACGTGACATTATGTTTGGTTCCCATCTGCTCCCCTGTACTCTGTCACTTGCTTCTTTCCTCCCCAGCT CATCATTATTACATGCGTTCTTTACTCTCTCTAGTTCCCTGATTCCTTAAGATCTCTCCCTTCCAGGGAT GGAGAGATGGCTCAGCAGTTAAGAGCCCTGGCTTCTCTTGCAGAGGACCCAGGCTTGGTTCTCAGCATCT ACATGGTGATGCAAAAGCATTCCTCTGGCCTCAGCAGGCACCAGGCATTCAGCACACAAACATTAAGGAA ACACTCATACACATAAAATAAAAATAAATAAATACTTTCAAAATGTTCTCCTCCTTCTCTCTCATAGTCC TCGTTCTGGTTTCCTCACACACATGCATATACACACATACACACATATATACACATATACACATATACAC ACACATATACACACATATACACATACATACACAACACACACATACACTCACAACACACACATACACTCAC ATATACACATACATACACAAATGTATATATACACACACATACACACATATACACACATGCACACACATAC ACACACAAACAAACATATACTTTTACATTATGGCCCCACATGTGAAAGAAAACATGTACTATTTGTCTGA GTTTGGCTTATGAACATAATAATCTCCAGCTTCACACACTTTCTATAAACATAATGATTTCCTTTTTCTT TAAACTCCCATTGTGAATTCGTAGCACATTTTCTCTACCCATTCATCTGTTGATGGACATCGTGGCTGCT TCCACTTCTTGACTATGATGAATAGAGCTTCTATAGACACGGATATGCAAATATCTCTGTGGTAGTACAC AGAGTTCTTTGGGCCTATACCCAGGTGTGGTATAGCTGGGCCGGATGATAGTTTCAGTCTCAGGTTCTTA AGAAACGTCCACAGAGGCTGCACACGTTTCATTCTTGTATTAATAACTAGCATGCATATACTATTGGTTC TTACCTTTTTTAAGTTTCACGTATTTTTCTGAGTTCCTGTTATGCTACAAAACCTAGCCTCTCACACTGT CCCTGTCCCTCAATAACTCAACCACAAACTTGGCTTTTTCTGACACCTGGATTCATCTTATCGATGCTTG TGTTGCAATGACTACTCCAGCATCATTCTCATACTGAGGATGTGGCACAAGAGCGTGTGTCCCTAGCCAG GCATGGAGGTGCAGAGAAGTAGAACTTACTTCCTCAGGCTCTGAGGCAAGAGCTTCTACACGCTGAGCCA TCTCCTCAGCCCTGTTTTGATCCCTAGTGTTCTGAGGCTTCATTTCTCTATGTGAGTCCTCTCTGCTTTA TTAGAGTGGGCAGTTAGCAGACATGGATGGCTACAAATTATTCAGTTTGGTGAAATTATTATTTTGTTGA TACTTTTTATGCTCTTGTGTTTTAAACTCCAGACTCTTGTGGGCCGAGATCTGAGTTCCTAATATCCATT AGTCATATTTTCACTTCTAGTTTCTGTAAAATTTGCTTGGTTGAATCTTTTAATCCTGTGTCATTTTAAA GCATTTTTATGGTGAAGATTATTTTTATTTTCCTTTTAAAAATATATATATATTTATTTATTTATTTATT TTAAACATGTTTGGCTTGCATGTGTGTCTGCATACTACCTACATGCCTGGTGCCCTCAGAGTCAAGAAGA GGGAGTCAGAGCCTCTGGAACTGGAGGGATGGATTGTTGAGAGCCTCCATGTGGGTGCTGGGAATAGAAC CTAGGTCCTCTGGAAGAGCAGCCAGTGCTCTTAACCATTGATCCATCTCTCCAGCACCTATTTATTTTCT TTTGAATCTTCCTAACAATTCTTTGTATTTCCATTATTCCTTTTTTTAAAATCTTCTTTTATGTGCTTCA TGAATGTTTCTCATTTCTTTCTCATCTTTAATGATATTAAGTGTAATCCTCTCCCTTCTATCGTCTCTGA ATCTTGGGAGTACAATGCTTTTGGTATCCCTTTTCATGCTCTCCCTTCCGGCATCTGACAGCCTTTGCCC GTCTGACAGTGAGGTGGGTGAGTGCCACAGCTGACTCTCAGCTCTCCATCAGTTTTCCAGTGTGAGGTGC TGGGCCCCAGTTCAGGGCTCTTGGTTTGTGCCATTTGTCATGAGCATCCTAATAGCAGAGTCTACGGATG TTTTCTTGGACTCATCAGACTCTCTTGACGAAGAGTCTCACTGCCCACATCTTGGGAGGGATAGCCCTAA TGGCCAGCCTGGGTGGTGCGTAGGGATTGACTCCATTTTCTAGAACACAGACTTTTCAGTTTACTCCTTT ATGGATGGGGGTTGGAGGCGGGGAATGTTGAGACAGATCTAACCCTGTAGCCCAGGCTTGCTTCACACTC ACAGCAATCCACTTGTCTCAGCTTCCAGAGTCCTGGGATGACTGGCATGAGTCGCTGTACCCAGCTCCCT CTCCGTCTTCTCGGTTGTGTTTTCCTATACTTCCATTGGGTTTGTTTCCCTATACTGTGTGTGTTAAGGG TCAACCTCCCAGATGTTGGGATGAGAGTTGCTGCATGGGATGGGAGCATTAAACTCTGTTTCCAGCCACT CTCAGCGATGCCTTATATTTCCTGTTGCTGAGCCTTTCTGGCATCCCATGGTTGCACATTAACTTTCGTC AGAGCTGCTACCACCCCTGCCTTATGTTACAGCCTTCCTCCTTCAGTTAGGCCAGGACTGCTCAGCCATT TTCTTTCCAGATTCCAAAGGGTGTCTTCATTACCTTCTCTTTCTCTTTTATATGTTTATGGGTTAAAAAA AAGAGAAATCTCATGGTGTCATCTCACTGGGGTTTTAGAGGGAGAGGTTACCCAGATGCTTGCAATCTGC AGTGTTAAATCTTAAATCCCTGGAAACAGTTTCAACTTGTGTATATTTACTTTTATGTTTTAGTTTTTGG AATGCATGTCACAAATATTTATGAGAATTCTAATGGTGTCTCCCTTGTGGTGTATTTATTTTGGAAAATA TTTTAGGTGTCTGGCTGAGAAGGCAGGAAATGTTGCATTTTTGAAGGACTCCACTGTCTTGCAGAATACT GACGGTATGTGAAGATGTTTCGCTTCCTCCTTCAGGAGCAGTCTTTAGCACCCTTAACATATCCTCATTT TGGGGTTGGTCTTTGGAAAGATAGTGTAACTATTTCAGACTTTTGACTAAAAGAATTCTCAAATATTTTA AATCTATCTTCCTGCCCCCCCTCTTTCTCTTTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTTTCTCTCT CAAGGCAGAGTCTCATTTTATAACTCAACCTTGAACTTACTACATACCTCACATGGTCCTTGAGCTCATG GAAGTCTTCCTCTCTTGGCAAACTGAGTTTGGGAAATTACAGGCATGAACCACACCAATTGGTGAGCTCT CAAGTCTCTACTGCTGTAGGCAGCATAGAAACACCTGTAGTGTCACAGTGTTTTTGAAGCAATGCACACA CACAGGTACACACATACAGATATACACACATTCACATAGACATACACACTTACATGCATATATACACAAA CACACATATATGCATATATACATACACACATTCACACAGATGTACATACACATATACATAAGCATACATA CTACACACACTGAGCTGCTACACATGAGTATATACATGAATATGTATGTACATACATACATCCACATATA CATTCACACAGGCATACACACACACACACACACACACACACACACACACACATACACACCTGTCATAGTT GGTAGCAGGCACATGAATGAAGAAACTCACGGTCTGTCTGTACCAGATGAAACAAGAACAGACAGTAATA ATAACTGAACCCATTCAAATAAGATTTTATCTTGAAGATGGGAATAGAGGCATTTCTTCTTCTCATCTCC ATATTAAAACCTTGGTCTAGAAACTCCTTGAGAGTCATTGAGTAAGAGTCCACACGAACATGTAGGTTAT GAAGAGAATGTAGCAGCCAACATTAGAATAAGATCCCCCCCTCTTCTGGTCGCTAGATTGGGAAAACAAA ATAGGGTACCCAACTCTTGCCAGCTGTTTGCTACTGCTCAAGTTGCTCTTCCTTCCAGGTCTCCTCAGCC TCCATGCCTCTGCCTCTGAAGGCAACCTGCTGCTTTGTTCAGTAAACTCCAGTCCAGCCCCTTCTTCCTT TCCCTTCTGCTTGCCTGTTGTCTCTGTGTGTGTGTGTTTGTGTGTGTGTGCTTGTGTGTGTGTGTGTGTG TGTATGTGTGTGTGTTTGTGTGTGTGTGTATGTGTGTGTGTGTTTGTGTGTGTGAGGAGACCTCTGAGAC CGAAATGCTCAGTATTAAGTAGAGAAAACCCAGGAAGGGGATGAAGTGTTTCCCAAGGAAGGAGAGTCAA GCAGGAGCTTGGCTAATCAGCCTTTGTGTGCACATGGTGTGCCACATTTACACCCAGAACTCATGGCAGC CTGTGCGTGATACTCCCTGAGTATCATCAGACTGATGTCAAATTTAACTTTAAGGCTCTTAGGTACTTAC TACCTATGTGTATTGGAGTTAACTAGACCTATTGATAAGCCCATGGAGTTGCGCATTTTTTCCATGTTGT ACTGAAATGAGACGGAAAAGACTGTCTGTGAAAGTCTGAGCTTGGAAATGCTCTAGGATTGGAAAGTTTT CCCACACCACACGATATCTAACATGTGGGGGAATCCTGCACACTTGACCTCATCTGATGAGTCAGAGCCA GACTGTGGTCACTCTGGCAACACTAGGCAATATGACCTTCAGGTGACACCTGCAATTTGCATATGAAACA TAATGGAATATTTTGTTTATTTTTGCATTACCATGACCACAGTATTTCATTATGTACATACAAATGTTCA AAAACTTGAACAAATTTCAAAGCAAAAGTAGTTCTGGTTCCAAACGTTTTGAATAAAGGGAACTCAACCT GCTTTCTTTCTTTGGAAACTACTAGAAAATATATTGCATAAATGGAACTCTTTTGTAAAAGGAAGGGGCA GGAAGATACATCAGTGGGTAAAGTGCATGTTATGCAAGCATTAAGACTTGAGTTCGCACTTGGGAGGCAG AGACAGGCAAATTTCTGAGTTCGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGCAGACAGAATGTCAT GGGCCTGCAGTCCCAGCTCTGAGGATGACAGGAATGGAGATAGGTGGATCCCCAGCCTTCTGGTCTAGCT GAATCAGTGAGCTCTGGGTTCTATGGAAAACTGTGTCTCAAAAAAAGAGCAAAGAAGCGATTGAGAAAGA TACCCATACGTAACTGCACAGGCAAGCATACCTGAACATATGTGTATGCACACATACAACACACACACAC ACACTTAAAAGAACATTTTTAAAAAGATTTACTTATTTACTCTGCATATAGTTTTCTGCTTGTAGGCCTG AAGAGGGCATCAGATCCCATTATAGATGGTTGTGAGCCACCATATGGTTGCTGGGAATTGAACTCAGGAC CTCTGGAAGAGCAGTCAGAGCTCTTAACCTCTGAGCTATCTCTTCAGCCCTTAAAAGAACATTTAACAGC TTGATTGGGATACAACTTGGTGATTTCAGAATCCACCCATTGTCTGTGTACAATCGAGTGGGCTCTAACA CACTCAGGATGGTGAAACCACCAATGCTACCATCCAACTCCACAACACTCATTTGAAAAACATGAAGCAC CCCATCTGTTGGCAGCCCACTGTCTTTGCTTTTTCAACATCTGCTTCCTGTTCCCATGGCTTTGTCTGTT CTCATAAATGCAACTATCATTTGTGGCCCTTTGTAGCTGGCTTCTTTATCATAGCAAAATTGTGAGAATC AGCCATGTTACCCCATGAATGAACTTCTTATTAAATAAGAATTTCCTTTATGACTAAGCTCGACATATTT GTCGCCTGAATAGCTTGTGAAGGATAAAGTTGCATTTTGTCTCCTGGACATCACCACAGCTTTCAAACTA GTCATACCTTGACCTAGCAGACTAGTTATGGCTAATGGTACCTGTCTTTTACCAGGGAAGAACACTGAAG AGTGGGCTAGGAACTTAAAGCTGAAGGACTTTGAGCTTTTGTGCCTTGATGACACCCGGAAACCTGTGAC TGAGGCTAAGAACTGCCACCTAGCCATAGCCCCAAACCATGCTGTAGTGTCTCGGACAGACAAGGTGGAA GTCCTTCAGCAGGTGCTGCTTGACCAACAGGTATGGACCAGCAGGGCTCCCAGCATGCTCTTCACTGCAC TGGCTCCTGCCGGGAGGGGGTTCATCCACTCAGACCTTTCTTTCTAGCCTTTCTGCTTGATCGATGGGGA TAGCGTTTGCTGCATGCTGTGAGTCTTTCTAGACTTCTGCCTGGATTCTCGGCTCCCAAACTTGAACTTT CTGACCTCTCCCTCCTGCTGCTTCCTCTAGTGACTCAGAGAGAGGGACGAACAGTGGCCAGGACAGAGGC TGTGAAGCAGTAAATCCAGAGTGTGTTCCATGTGCAGGAGACACGAGGTCTTTGTGCTCACAGATTAAAT ACTAGGAAAAGCAGGAATATTGAACATGTGGCGTTGTCTCTTCAAAAAAGAGATGTATGACCTATTTTTG CCAAGAAGCCTGGGAACAGGCGAGGTTAACAGCCTGGCGACTTCTACACTATCTGTATGGCCCAGCCATT CTGGATCCCAGACTATATGCCTGCCTCAGTCAGTCTATAACATTATCTTCTCTGAGCCTCTTATCCCTGA GCCTGGTTTTATAGAACCCATCTTTATGGAAGATGCCTTGTTCCTTACAAGAGTTATTCATGTAATCATG TCTTAAGCTTTGTTGTATACACAGGACAGTTTTTTCCCCCCTTAAATTGTACTGTGCTTAAATATGCCTG AAACAGACTACCCAGTGTCAGGGTTTTGAAGTTTGACCTAACACTGGCTACTGAGTTGTGTTGAACCAAA CTTCCTTCTTGCCTCAAACACAGTGCTGGCCCTGGTGTTTGTACGGAGCCCCGCGTCTGTGGATTTAGGG ATGACTGGGTTCTAGTGTTCCCTGTTCTAGTTTCTATTTTCCTATCACAGCATGTTGGCTCTGGGTACAC TTTTGAGGCCAACAGCCCTGAGTAAGGAGAAGGGACTGCAGCCGAGAGAGGCTCAATGCCCTGTGGTGGC CCAGTTCACAGAAACATGGGAATTTTTAAATTACCCGTTTGGACAGCTGGACACTACAATGTTCTAGATG ATCAGCTTTTGAGGGAAAATCTCAAAACATCTTCGTCTGAACTCTAATCACCAGCAGGAAGTGATAACAG ATTTAGTAAAAGGAAAGATATGGGAGGCAAGGTCAGAAGTGGTGTGAGGCTCCAGCCCTGGCTGAAGTGG GCATGCTGCCAGGGGGAGCACAGCACTGGGAGCACGGTGCTGGGAAGAGTCATCCCAGGCAGAGAAAAGA ACACTGCTGCTTTTTAGCTCTGCTGTTTACCCAAAATGGAGGAACCCTTATCATTCCCGCCCCCCACTCT CTGACTACTCACAGAACAGTCCACTCTCTTGACACAGGTTCAGTTTGGGAGAAATGGACAGAGGTGTCCA GGAGAGTTTTGCCTGTTCCAGTCTAAAACCAAAAACCTTCTGTTCAATGACAACACTGAGTGTCTGGCCA AGATCCCCGGCAAAACCACATCGGAGAAGTATCTGGGAAAGGAGTACGTCATAGCGACCGAGCGCCTGAA GCAGTGCTCCAGCTCCCGTGAGTGGGTTCTAGATATAATTGCCAGGGACCAACATGGGAGAAGCTCCGGG TTCGGAGGCCAAAATGCATGGAGAAGTGCAAGTTCTTAAAACCTTACAATGTGGAGCCAAAGAGATGGCT CAGGGGGTAAGAGACGGACTGCTCTTCCAGATGACACCGGTTCAGTCCAGAGCAACTGTCCATCTGGCAG CTCGCAGCTGTCTGTAACTCCAGTTCCAGGGAAGCTGACACTTCTTCTGGCCTCTGTAGGCACCAGGCCG GCATGTAGTCATCAGACATCCGTGCAGGCAAAGCGTTCACACACATGACACAATAAGTTCAAATGTAGGA AAGTATAGTGATAATAATTCCATTCTCGAAAACTGTTGGGAAGAGTGGCTGAAGAGTTAGTAGATTATTT GCCTCCCGTGCACAAAGCCCTGGGTCCAGCCTCTAGCATGGCATAAACTGTGTGGTGCACACCTGTAACC CCAGAACTCGAGAGGTAGAAACAAGACAACCAGATGTTCTAAGTCATCCTTGGCTACATATGGAGATCGA AACCAGCCTGGGGGACATAAAACCCTATCTCAAAAAAATTTCAAATACCTGTTATACATATATACATGCA TACACACACACACACATACACACACACATACACACACACACATATACACACACACATACACACACACATA TACACACACACATATACGCGCACACACACACATATACACACACATATATACACACACATATATACACACA CACACATACATACAGATTGCAATCCCCAAGCATCCCCACGCACCGATAGCCTGTGCACAGTAGCCCCTTG AATCCCTACATCTGGTGGTCCCTACTCTGAGAGGCAGAGGCATGGCAGATGTTGTGGTCCAGGAGTCGGA GCAGGCCCAGAATCTCCTTGTCAGTTGTTCTCAACTTAGGACAATTTTATCCCCAAAGCAGACATTTGGC CATGCCTAGAAACATCTTTGATGACCACAGTTTAGTAGGTGCTTCTGGTAGGAGGCAGTAGAGGTTGGGG TCATTAGCAAGCAATGCATAGGATACCACAGCCTACGAAAAAGGATTGTCAGGTTCCCTTTGCTGGTGGG CCTGAGGTGAGGAGCCACGATCTGTATTGAGCAACGTCTCAGGCTGTGGCAGGTGTGGTCCACCCTGGGT GACCCAGGGCTATTGATGAACCTGTGAACAGAAGGCACTGGTGCAGAGGCCATGTGCTCGAGGACAGAGC TGTCTGCCCATGTTCTCCCAGCTCATGGTGAAGTGGCTCACACAAGGCAGGTGCTCAGCCAGTATTTGCA GATGAAGGATAAGCAAACAGATCAGCCTTCATCGTGCACCATTCAGGAGCCAGAGGACCAGTGTCTTGTG AACTTGAGTGGTGTGAACTGATTCACTTAGAGGTGGAGTCACATGACCCTCAAGTCAGAGCAGGGAGTTG CTGTGGCCCAGGGCGCCACAGTTGCTAGAAGAGGTGGTAGAAAACATGTGGACGGACGAGGCAAGCCCTG ACTCACGATGGCAGGACAAGGGCTTGGACACTGTGTTTGAAACCTCTTTGATGTATAGATTTGGGGGGAA ATCTGTGTCTGAATGACCTGCTTTCTGTGTCCCACAGCACTCCTGGAAGCCTGCGCTTTTCTTACCCAGT GAAAACACTGAGCAAATAGCAGAACCTTCCCAGGAAGTCTCATCCCGGAGCCACGGTCCGGGGGCCTTCA GACCATCTGGTCTCCTCACTCCCTGCTGTCACTTTAGGTAGAAATAAAATGAAGTAGTGTTGAGTTTCTC GTCATATGAGGTGTCACTCATTGCTCCTCAAAGTTTATACTGGAATCCTGTGTCTGACCACTTGCCTGCA CACTTGGACTTGCTTTTGGAGATTCTCGGTCCAGCCCTTCCTTTATCCTGAAGTTTCAGGCATGGTGCCC ATTGACCTGGAGTCTGGGGACAACCACGTGATGTTTCTGTCCCCAGGGGATCTGGTTTCTTCACATAGAG ATCACAGTACATGTGCATCTGTGTGCCCCAGATCATGCGTGCGTATGGGTGACCATGTATGCCTCAACAT AAGTTTGCACATGACTGTGTGCACTCCTTGCACTGATGTTTGCATAGGACTGTGTACTTGCACTAATGCT TATGTGTGTGTGTGCACACACGTGTGAAAGCCAGAGGACAATCTGGAATGTCACCATCCACCTTTTTTAA AAATTAAATTTATTTATTTACATCC >gi|31560677|ref|NP_032548.2| lactotransferrin precursor [Mus musculus] MRLLIPSLIFLEALGLCLAKATTVQWCAVSNSEEEKCLRWQNEMRKVGGPPLSCVKKSSTRQCIQAIVTN RADAMTLDGGTMFDAGKPPYKLRPVAAEVYGTKEQPRTHYYAVAVVKNSSNFHLNQLQGLRSCHTGIGRS AGWKIPIGTLRPYLNWNGPPASLEEAVSKFFSKSCVPGAQKDRFPNLCSSCAGTGANKCASSPEEPYSGY AGALRCLRDNAGDVAFTRGSTVFEELPNKAERDQYKLLCPDNTWKPVTEYKECHLAQVPSHAVVSRSTND KEEAIWELLRQSQEKFGKKQASGFQLFASPSGQKDLLFKESAIGFVRVPQKVDVGLYLTFSYTTSIQNLN KKQQDVIASKARVTWCAVGSEEKRKCDQWNRASRGRVTCISFPTTEDCIVAIMKGDADAMSLDGGYIYTA GKCGLVPVLAENQKSSKSNGLDCVNRPVEGYLAVAAVRREDAGFTWSSLRGKKSCHTAVDRTAGWNIPMG LLANQTRSCKFNEFFSQSCAPGADPKSNLCALCIGDEKGENKCAPNSKERYQGYTGALRCLAEKAGNVAF LKDSTVLQNTDGKNTEEWARNLKLKDFELLCLDDTRKPVTEAKNCHLAIAPNHAVVSRTDKVEVLQQVLL DQQVQFGRNGQRCPGEFCLFQSKTKNLLFNDNTECLAKIPGKTTSEKYLGKEYVIATERLKQCSSSPLLE ACAFLTQ Vaximmutor lysA Mycobacterium tuberculosis H37Rv 886960 15608433 Rv1293 BX842576 NP_215809 1HKW 83332 1448027 1449370 ? diaminopimelate decarboxylase LysA 5.07 42101.78 447 Rv1293, (MTCY373.13), len: 447 aa. Probable lysA, diaminopimelate decarboxylase (EC 4.1.1.20) (see citation below), almost identical to DCDA_MYCTU|P31848. Contains PS00878 Orn/DAP/Arg decarboxylases family 2 pyridoxal-P attachment site, PS00879 Orn/DAP/Arg decarboxylases family 2 signature 2. BELONGS TO FAMILY 2 OF ORNITHINE, DAP, AND ARGININE DECARBOXYLASES. >gi|57116681:1448027-1449370 Mycobacterium tuberculosis H37Rv, complete genome TGTGAACGAGCTGCTGCACTTAGCGCCGAATGTGTGGCCGCGCAATACTACTCGCGATGAAGTCGGTGTG GTCTGCATCGCAGGAATTCCACTGACGCAGCTCGCCCAGGAGTACGGGACCCCGCTGTTCGTCATCGACG AGGACGACTTTCGCTCGCGCTGCCGAGAAACCGCCGCGGCCTTTGGAAGTGGGGCGAACGTGCACTATGC CGCCAAGGCGTTCCTGTGCAGCGAAGTAGCCCGGTGGATCAGCGAAGAAGGGCTCTGTCTGGACGTTTGC ACCGGTGGGGAGTTGGCGGTCGCGCTGCACGCTAGCTTTCCGCCCGAGCGAATTACCTTGCACGGCAACA ACAAATCGGTCTCAGAGTTGACCGCTGCGGTCAAAGCCGGAGTCGGCCATATTGTCGTCGATTCGATGAC CGAGATCGAGCGCCTCGACGCCATCGCGGGCGAGGCCGGAATCGTCCAGGATGTCCTGGTGCGTCTCACC GTCGGTGTCGAGGCGCACACCCACGAGTTCATCTCCACCGCGCACGAGGACCAGAAATTCGGGTTATCGG TGGCCAGCGGCGCGGCCATGGCAGCGGTGCGGCGCGTTTTCGCCACTGATCACCTGCGCCTGGTTGGGCT ACACAGCCACATCGGTTCGCAGATCTTCGACGTGGACGGCTTCGAACTCGCCGCGCACCGTGTCATCGGC CTGCTACGCGACGTCGTCGGCGAGTTCGGTCCCGAAAAGACGGCACAGATCGCGACCGTCGATCTCGGTG GCGGCTTGGGCATCTCGTATTTGCCGTCCGACGACCCACCGCCGATAGCCGAGCTCGCGGCCAAGCTGGG TACCATCGTGAGCGACGAGTCAACGGCCGTGGGGCTGCCGACGCCCAAGCTCGTTGTGGAGCCCGGACGC GCCATCGCCGGACCGGGCACCATCACGTTGTATGAGGTCGGCACCGTTAAGGACGTCGATGTCAGCGCCA CAGCGCATCGACGTTACGTCAGTGTCGACGGCGGCATGAGCGACAACATCCGCACCGCGCTCTACGGCGC GCAGTATGACGTCCGGCTGGTGTCTCGAGTCAGCGACGCCCCGCCGGTACCGGCCCGTCTGGTCGGAAAG CACTGCGAAAGTGGCGATATCATCGTGCGGGACACCTGGGTGCCCGACGATATTCGGCCCGGCGATCTGG TTGCGGTTGCCGCCACCGGCGCTTACTGCTATTCGCTGTCGAGTCGTTACAACATGGTCGGCCGTCCCGC TGTGGTAGCGGTGCACGCGGGCAACGCTCGCCTGGTCCTGCGTCGGGAGACGGTCGACGATTTGCTGAGT TTGGAAGTGAGGTG >gi|15608433|ref|NP_215809.1| diaminopimelate decarboxylase LysA [Mycobacterium tuberculosis H37Rv] MNELLHLAPNVWPRNTTRDEVGVVCIAGIPLTQLAQEYGTPLFVIDEDDFRSRCRETAAAFGSGANVHYA AKAFLCSEVARWISEEGLCLDVCTGGELAVALHASFPPERITLHGNNKSVSELTAAVKAGVGHIVVDSMT EIERLDAIAGEAGIVQDVLVRLTVGVEAHTHEFISTAHEDQKFGLSVASGAAMAAVRRVFATDHLRLVGL HSHIGSQIFDVDGFELAAHRVIGLLRDVVGEFGPEKTAQIATVDLGGGLGISYLPSDDPPPIAELAAKLG TIVSDESTAVGLPTPKLVVEPGRAIAGPGTITLYEVGTVKDVDVSATAHRRYVSVDGGMSDNIRTALYGA QYDVRLVSRVSDAPPVPARLVGKHCESGDIIVRDTWVPDDIRPGDLVAVAATGAYCYSLSSRYNMVGRPA VVAVHAGNARLVLRRETVDDLLSLEVR Virmugen A lysA mutant, in combination with a secA2 mutation, is highly attenuated in mice and induced significant protection from challenge with wild type M. tuberculosis [Ref1809:Hinchey et al., 2011]. mce-2 Mycobacterium tuberculosis CDC1551 925014 15839991 MT0618 AE000516 NP_335028 83331 688269 689483 + virulence factor 4.96 39757.76 404 identified by similarity to GP:5814111; match to protein family HMM PF02470; match to protein family HMM TIGR00996 >gi|50953765:688269-689483 Mycobacterium tuberculosis CDC1551, complete genome GGTGCCAACGCTGGTGACGAGGAAGAACCGACGTGCGTGGCTGTATGTGGAGGGTGTTGTCCTGCTGTTG GTGGGCGCGTTGGTGCTCGTATTGGTGTACAAGCAGTTTCGTGGGGAATTCACGCCGAAGACCGAGCTGA CTATGGTCGCCTCCCGGGCTGGGCTGGTTATGGAAGCTGGATCCAAAGTCACCTACAACGGGGTGGAGAT CGGCCGGGTGGGCAGCATTTCGGAGATTGAGCGTGACGGCCGGCCGGCGGCGAAGCTGGTTTTGGACGTG AATCCTCGCTACATCAGCCTGATTCCGGTCAATGTGGTGGCCGATATCGAGGCGGCCACCCTGTTCGGCA ACAAGTATGTTGCGCTGTCCGCGCCGAAAATTCCTCAACAGCAGCGGATTTCCTCACATGACGTGATTGA TGTGGGGTCGGTGACCACCGAATTCAACACGTTGTTCGAGACGATCACCTCGATCGCCGAGAAGGTGGAT CCGATCGAGCTGAACGCGACGCTGTCCGCGGTAGCACAGGCGCTGGATGGGCTGGGCGGCAAGTTCGGTG AGTCGATCGTTAATGGCAATCAGATTCTGGCGCAATTAAATCCGCGGCTGCCGCAGCTCGGCTATGATGT TCGGCGGTTGGCGGATCTCGGTGAGGTCTATGTCGATGCTTCGCCGGATCTGTGGTCCTTTCTGCAGAAC GCACTGACCACTGCGCGCACATTGACCAGCCAACAGCGCGATCTGGATGCCGCGTTGTTGGCGGCTACGG GTGCGGGCAACACCGGTGAAGACGTTTTTGCTCGAGGCGGGCCGTATCTTGCGCGCGCAGCCGCCGATCT GGTGCCCACCGCTACGCTGCTGGACACCTACAGTCCCGAACTGTTCTGCATGATCCGCAACTTTCACGAC GCTGCGCCCAAAGTCGCGGACGCGGTGGGCGGCAACGGCTATTCGCTAGCGGCCGCCGGAACGATTTTGG GAGCACCCAATCCCTATGTCTATCCGGACAATCTGCCGCGGGTGAATGCCCACGGTGGACCCGGGGGCCG ACCGGGCTGCTGGCAGACGATCACCCGGGAGCTGTGGCCGGCACCCTATCTGGTGATGGACACCGGTGCC AGCCTCGCACCGTACAACCACGTCGAGCTCGGCCAACCGATGTTCACTGAATACGTATGGGGACGCCAAT ACGGAGAGAACACGATCAACCCATG >gi|15839991|ref|NP_335028.1| virulence factor [Mycobacterium tuberculosis CDC1551] MPTLVTRKNRRAWLYVEGVVLLLVGALVLVLVYKQFRGEFTPKTELTMVASRAGLVMEAGSKVTYNGVEI GRVGSISEIERDGRPAAKLVLDVNPRYISLIPVNVVADIEAATLFGNKYVALSAPKIPQQQRISSHDVID VGSVTTEFNTLFETITSIAEKVDPIELNATLSAVAQALDGLGGKFGESIVNGNQILAQLNPRLPQLGYDV RRLADLGEVYVDASPDLWSFLQNALTTARTLTSQQRDLDAALLAATGAGNTGEDVFARGGPYLARAAADL VPTATLLDTYSPELFCMIRNFHDAAPKVADAVGGNGYSLAAAGTILGAPNPYVYPDNLPRVNAHGGPGGR PGCWQTITRELWPAPYLVMDTGASLAPYNHVELGQPMFTEYVWGRQYGENTINP Virmugen Mce-2 and mce-3 mutants in Mycobacterium tuberculosis is attenuated and provide protection against challenge for mice [Ref1932:Aguilar et al., 2006]. mce-3 Mycobacterium tuberculosis CDC1551 923491 15841441 MT2018 AE000516 NP_336478 83331 2206659 2207936 + virulence factor 6.51 41527.31 425 identified by similarity to GP:5814111; match to protein family HMM PF02470; match to protein family HMM TIGR00996 >gi|50953765:2206659-2207936 Mycobacterium tuberculosis CDC1551, complete genome CATGAGACGCGGGCCGGGTCGACACCGTTTGCACGACGCGTGGTGGACGCTGATCCTGTTCGCGGTGATC GGGGTGGCTGTCCTGGTGACGGCGGTGTCCTTCACGGGCAGCTTGCGGTCGACTGTGCCGGTGACGCTGG CGGCCGACCGCTCCGGGCTGGTGATGGACTCCGGCGCCAAGGTCATGATGCGCGGTGTGCAGGTCGGCCG GGTCGCCCAGATCGGTCGGATCGAGTGGGCCCAGAACGGGGCGAGCCTCAGACTGGAGATCGACCCCGAC CAGATCCGGTACATCCCGGCCAATGTCGAGGCACAGATCAGCGCCACCACCGCATTCGGTGCCAAGTTCG TCGACCTGGTGATGCCGCAAAACCCAAGTCGTGCACGGCTGTCCGCTGGGGCGGTACTGCATTCGAAGAA CGTCAGCACGGAAATCAACACCGTCTTCGAAAACGTCGTCGACCTGCTCAACATGATCGACCCGCTGAAA CTGAACGCCGTGCTGACCGCGGTCGCCGACGCCGTTCGCGGGCAAGGTGAACGGATAGGCCAGGCCACCA CCGACCTCAACGAGGTGCTGGAGGCACTCAACGCACGCGGCGACACCATCGGCGGCAACTGGCGATCGCT CAAGAACTTCACCGACACCTATGACGCGGCCGCCCAAGACATCCTGACGATCCTGAACGCCGCCAGCACC ACCAGTGCGACCGTCGTGAATCATTCGACGCAGCTGGATGCCTTGCTACTCAACGCCATCGGACTATCCA ACGCTGGCACCAACCTGCTTGGCAGCAGCCGAGACAATCTCGTCGGCGCGGCCGACATCCTGGCGCCGAC CACGAGCCTGCTGTTCAAGTACAACCCCGAATACACCTGCTTCCTGCAGGGCGCCAAGTGGTATCTCGAC AACGGCGGCTATGCGGCCTGGGGCGGGGCCGACGGGCGCACGCTACAACTCGATGTGGCGCTACTGTTCG GCAACGACCCCTATGTCTATCCGGACAACCTGCCGGTTGTCGCGGCCAAGGGGGGTCCCGGCGGAAGGCC GGGATGCGGGCCATTGCCGGATGCCACCCACAACTTCCCGGTGCGCCAGCTGGTCACCAACACCGGATGG GGAACCGGGCTGGACATCCGGCCCAACCCCGGCATCGGGCATCCCTGCTGGGCCAACTACTTCCCGGTGA CCCGCGCGGTGCCCGAGCCGCCGTCGATCCGTCAGTGCATCCCCGGGCCGGCGATCGGGCCCAACCCCGC GGCGGGGGAGCAGCCATG >gi|15841441|ref|NP_336478.1| virulence factor [Mycobacterium tuberculosis CDC1551] MRRGPGRHRLHDAWWTLILFAVIGVAVLVTAVSFTGSLRSTVPVTLAADRSGLVMDSGAKVMMRGVQVGR VAQIGRIEWAQNGASLRLEIDPDQIRYIPANVEAQISATTAFGAKFVDLVMPQNPSRARLSAGAVLHSKN VSTEINTVFENVVDLLNMIDPLKLNAVLTAVADAVRGQGERIGQATTDLNEVLEALNARGDTIGGNWRSL KNFTDTYDAAAQDILTILNAASTTSATVVNHSTQLDALLLNAIGLSNAGTNLLGSSRDNLVGAADILAPT TSLLFKYNPEYTCFLQGAKWYLDNGGYAAWGGADGRTLQLDVALLFGNDPYVYPDNLPVVAAKGGPGGRP GCGPLPDATHNFPVRQLVTNTGWGTGLDIRPNPGIGHPCWANYFPVTRAVPEPPSIRQCIPGPAIGPNPA AGEQP Virmugen Mce-2 and mce-3 mutants in Mycobacterium tuberculosis is attenuated and provide protection against challenge for mice [Ref1932:Aguilar et al., 2006]. MPT64 Mycobacterium tuberculosis H37Rv VO_0012367 885925 15609117 Rv1980c A42312 NP_216496 2HHI 83332 2223342 2224028 - immunogenic protein Mpt64 4.6 23267.4 228 Rv1980c, (MT2032, MTCY39.39), len: 228 aa. mpt64 (alternate gene name: mpb64), immunogenic protein (alternate gene name: mpb64) (see citations below), identical to MPT64|MPB64 from Mycobacterium bovis (228 aa). Similar to Rv3036c|MTV012.51c from Mycobacterium tuberculosis. Exported protein containing a N-terminal signal sequence: see notes below about proteomics.; mpb64 >NC_000962.3:2223342-2224028 Mycobacterium tuberculosis H37Rv, complete genome TCTAGGCCAGCATCGAGTCGATCGCGGAACGTGGGACCAATACCTGGGTTGGGCCGGCTGCTTCGGGCAG CAACTCCCCCGGGTTGAAGAAGAAAATCACCCCGTCGTTCGTGACTGCGAAGTTCTGATAATTCACCGGG TCCAAGCCGGCATTCGGCGCTATCGATACCTGTTGTCCGGTCTGCTTGCTCAGTTCACCTTGCACAATGG GGAAGACGACTGGCAGCGGATCGGTGTCAGCCTGCCACAGCGTGTCATAGGTGATTGGCTTGCGATAGGC CTGGTCCCAATCGAAGGCCTTGTACGTGGTCGTTGGGTGCGTGCCGCCGGCGTTCTGGTAGACCTTGAGC ACCACGGCCTGCGTACCACGCGGCGGTATCGCGGACTGGTATGTGGCCGAGGTGATATTCAATTCGTAGG GGGCTTCGCGTGGAGTGGACGATGTGGCCGCGCTGAGGAACTTGTCGCGCGTCTGGGCGATGTAATTTTC CAGCGACTTCTGGTCGGGGTAGTAACTGGGCAGGCTGATGTTGATGTTGTAGGCCGGGTCGGACATTTGA ATCTGGCACGCCTGGCCGGTATCGGTGCCTTTCAACTCCTCGCAGTAGGTCTTGGGCGCGGCCGTGGCCA CACCCGAACAACAGAGCAAAACGACAGCCGTGACCAGCATGAAGATCTTGATGCGCA >NP_216496.1 immunogenic protein Mpt64 [Mycobacterium tuberculosis H37Rv] MRIKIFMLVTAVVLLCCSGVATAAPKTYCEELKGTDTGQACQIQMSDPAYNINISLPSYYPDQKSLENYI AQTRDKFLSAATSSTPREAPYELNITSATYQSAIPPRGTQAVVLKVYQNAGGTHPTTTYKAFDWDQAYRK PITYDTLWQADTDPLPVVFPIVQGELSKQTGQQVSIAPNAGLDPVNYQNFAVTNDGVIFFFNPGELLPEA AGPTQVLVPRSAIDSMLA Protective antigen DNA vaccines encoding secreted proteins Ag85B and MPT64 protect mice from the tuberculosis H37Rv strain [Ref1478:Luo et al., 2004]. *Note* MPT64 in combination with Ag85B was protective, DNA vaccine of just MPT64 was mildly protective in mice. mpt70 Mycobacterium tuberculosis H37Rv 887724 15610012 Rv2875 AL123456 NP_217391 83332 3187029 3187610 + major secreted immunogenic protein Mpt70 Also known as mpb70 Protective antigen MPT83 Mycobacterium tuberculosis H37Rv VO_0012409 887155 15610010 Rv2873 AL123456 NP_217389 83332 3183904 3184566 + cell surface lipoprotein 4.73 20581.44 220 Rv2873, (MTCY274.04), len: 220 aa. mpt83 (alternate gene name: mpb83), cell surface lipoprotein (see citations below). Also similar to upstream ORF Q50769|MP70_MYCTU|MPT70|MPB70|Rv2875|MT2943|MTCY274.06 which is also known as MAJOR SECRETED IMMUNOGENIC PROTEIN MPT70 PRECURSOR from Mycobacterium tuberculosis (193 aa), FASTA scores: opt: 806, E(): 2.7e-38, (70.25% identity in 185 aa overlap). BELONGS TO THE MPT70 / MPT83 FAMILY. ATTACHED TO THE MEMBRANE BY A LIPID ANCHOR.; mpb83 >NC_000962.3:3183904-3184566 Mycobacterium tuberculosis H37Rv, complete genome GATGATCAACGTTCAGGCCAAACCGGCCGCAGCAGCGAGCCTCGCAGCCATCGCGATTGCGTTCTTAGCG GGTTGTTCGAGCACCAAACCCGTGTCGCAAGACACCAGCCCGAAACCGGCGACCAGCCCGGCGGCGCCCG TTACCACGGCGGCAATGGCTGACCCCGCAGCGGACCTGATTGGTCGTGGGTGCGCGCAATACGCGGCGCA AAATCCCACCGGTCCCGGATCGGTGGCCGGAATGGCGCAAGACCCGGTCGCTACCGCGGCTTCCAACAAC CCGATGCTCAGTACCCTGACCTCGGCTCTGTCGGGCAAGCTGAACCCGGATGTGAATCTGGTCGACACCC TCAACGGCGGCGAGTACACCGTTTTCGCCCCCACCAACGCCGCATTCGACAAGCTGCCGGCGGCCACTAT CGATCAACTCAAGACTGACGCCAAGCTGCTCAGCAGCATCCTGACCTACCACGTGATAGCCGGCCAGGCG AGTCCGAGCAGGATCGACGGCACCCATCAGACCCTGCAAGGTGCCGACCTGACGGTGATAGGCGCCCGCG ACGACCTCATGGTCAACAACGCCGGTTTGGTATGTGGCGGAGTTCACACCGCCAACGCGACGGTGTACAT GATCGATACGGTGCTGATGCCCCCGGCACAGTA >NP_217389.1 cell surface lipoprotein [Mycobacterium tuberculosis H37Rv] MINVQAKPAAAASLAAIAIAFLAGCSSTKPVSQDTSPKPATSPAAPVTTAAMADPAADLIGRGCAQYAAQ NPTGPGSVAGMAQDPVATAASNNPMLSTLTSALSGKLNPDVNLVDTLNGGEYTVFAPTNAAFDKLPAATI DQLKTDAKLLSSILTYHVIAGQASPSRIDGTHQTLQGADLTVIGARDDLMVNNAGLVCGGVHTANATVYM IDTVLMPPAQ Protective antigen A single DNA vaccine encoding the MPT-83 tuberculosis antigens evoked partial protection against an aerogenic challenge with M. tuberculosis Erdman in the mouse model of pulmonary tuberculosis [Ref1534:Morris et al., 2000]. Msh1 Mycobacterium tuberculosis H37Rv 887398 15609809 Rv2672 AL123456 NP_217188 83332 2987681 2989267 + mycobacterial secreted hydrolase 1 4.72 49550.79 528 >NC_000962.3:2987681-2989267 Mycobacterium tuberculosis H37Rv, complete genome AATGGCTACTGTGGTCGGCATGAGTCGGCCCATGACGTCAACCGCGATGTTGGTCGCGCTGACCTGCTCG GCGACAGTGCTGGCCGCATGCGTCCCGGCGTTCGGCGCCGACCCGCGGTTCGCGACCTACTCGGGCGCAG GACCGCAAGGCGCAGCCACCACGACACCACCGCCGGCTGGCCCACCACCGCTCGCCGCACCCAAGAACGA CTTGTCGTGGCACGACTGCACGTCACGGGTGTACTCGAATGCTGGGATCCCAGCAGCGCCCGGCGTCAAG CTGGAATGCGCAAGCTATGACACCGACCTCGACCCGCTCGTCGGCGGGTCCACAGCGGTAAGCATCGGCG TAGTGCGCGCGCGCTCCAACCAGACCCCGAGCGACGCAGGACCCCTGGTGTTCACCACCGGCTCCGACCT ACCCTCGTCGACGCAGTTGCCGGTCTGGCTGGCACACGCGGGCATCGATGTGCTCCGCAGCCACCCCATT GTCGCCGTCGACCGCCGCGGCATGGGCATGTCGAGCCCAATCGACTGCCGCGATCACTTTGACCGCGACG AGATGCGTGATCAGGCGCAATTCCAGGCTGGCGACGATCCGGTGGCCAACCTTTCCGACATCTCCAACAC CGCCACCACCGACTGCACCGACGCCATCGCGCCAGGCGAGTCCGCCTACGACAACACCCACGCCGCCTCG GATATCGAGCGCTTACGCAAACTCTGGGACGTCCCTGCCCTCGCCTTCGTCGGCATTGGCAACGGCACCC AAGTGGCGCTGGCCTACGCAGCATCGCGTCCCGACAACGTCGCCAGACTGATCCTCGACTCCCCAATCGC GTTGGGGGTCTCTGCCGAAGCCGCCGCCGAGCAACAGGTCCAGGGCCAACAGGCGGCGCTGGACGCATTC GCTGCGCAATGTGTCGCGGTGAACTGCGCGCTGGGCTCCCATCCGAAAGGCGCGGTCAGCGCGCTGCTGT CGGCCGCCCGGTCCGGTGATGGGCCCGGCGGCGCGTCGGTGGCGGCTGTCGCCAACGCCGTCGCCACCGC GTTGGGCTTCCCCGACAGTGGCCGGGTCGATAGCACCACGAAATTGGCCGACGCGCTGGCCGCGGCCCGC TCCGGGGACATGAACTTGCTGTCCGCCCTGATCAACCGCGCCGATACCACCCGGGATACGGACGGTCAGT TCATCAGCTCGTGCAGCGATGCGGTCAACCGCCCGACACCGGACCGGGTGCGCGAGCTGGTGGTGGCTTG GGGGAAGCTCTACCCGCAGTTCGGCGCCGTCGCGGCGCTCAACCTGGTGAAATGCGTGCACTGGCCCAGC AGTTCGCCGCCGCAGCCACCGAAAGACCTCAAGGTCGACGTGCTGTTGCTCGGTGTGCAAAACGACCCGA TCGTGGGCAACGAAGGGGTCGCCGCGACCGCCGCCACGGCCATCAACGCCAACGCCGCCAGCAAGCGGGT GATGTGGCAAGGTATTGGCCACGGCGCCAGCATCTACTCGTCCTGCGCGGTGCCGCCACTCGTCGCCTAC CTGGACACTGGCAAGCTGCCTGACACCGACACCTATTGCCCCGCCTG >NP_217188.1 protease [Mycobacterium tuberculosis H37Rv] MATVVGMSRPMTSTAMLVALTCSATVLAACVPAFGADPRFATYSGAGPQGAATTTPPPAGPPPLAAPKND LSWHDCTSRVYSNAGIPAAPGVKLECASYDTDLDPLVGGSTAVSIGVVRARSNQTPSDAGPLVFTTGSDL PSSTQLPVWLAHAGIDVLRSHPIVAVDRRGMGMSSPIDCRDHFDRDEMRDQAQFQAGDDPVANLSDISNT ATTDCTDAIAPGESAYDNTHAASDIERLRKLWDVPALAFVGIGNGTQVALAYAASRPDNVARLILDSPIA LGVSAEAAAEQQVQGQQAALDAFAAQCVAVNCALGSHPKGAVSALLSAARSGDGPGGASVAAVANAVATA LGFPDSGRVDSTTKLADALAAARSGDMNLLSALINRADTTRDTDGQFISSCSDAVNRPTPDRVRELVVAW GKLYPQFGAVAALNLVKCVHWPSSSPPQPPKDLKVDVLLLGVQNDPIVGNEGVAATAATAINANAASKRV MWQGIGHGASIYSSCAVPPLVAYLDTGKLPDTDTYCPA Protective antigen This is used for protein fusion vaccine development [Ref6780:Junqueira-Kipnis et al., 2022]. Mtb72F Mycobacterium tuberculosis VO_0011173 283801990 32630 ? 4.95 66088.21 799 Description of Artificial Sequence:tri-fusion protein MTB72F (Ra12-TbH9-Ra35 or MTB32-MTB39 fusion) >CBI85484.1 unnamed protein product [synthetic construct] MHHHHHHTAASDNFQLSQGGQGFAIPIGQAMAIAGQIRSGGGSPTVHIGPTAFLGLGVVDNNGNGARVQR VVGSAPAASLGISTGDVITAVDGAPINSATAMADALNGHHPGDVISVTWQTKSGGTRTGNVTLAEGPPAE FMVDFGALPPEINSARMYAGPGSASLVAAAQMWDSVASDLFSAASAFQSVVWGLTVGSWIGSSAGLMVAA ASPYVAWMSVTAGQAELTAAQVRVAAAAYETAYGLTVPPPVIAENRAELMILIATNLLGQNTPAIAVNEA EYGEMWAQDAAAMFGYAAATATATATLLPFEEAPEMTSAGGLLEQAAAVEEASDTAAANQLMNNVPQALQ QLAQPTQGTTPSSKLGGLWKTVSPHRSPISNMVSMANNHMSMTNSGVSMTNTLSSMLKGFAPAAARQAVQ TAAQNGVRAMSSLGSSLGSSGLGGGVAANLGRAASVGSLSVPQAWAAANQAVTPAARALPLTSLTSAAER GPGQMLGGLPVGQMGARAGGGLSGVLRVPPRPYVMPHSPAAGDIAPPALSQDRFADFPALPLDPSAMVAQ VGPQVVNINTKLGYNNAVGAGTGIVIDPNGVVLTNNHVIAGATDINAFSVGSGQTYGVDVVGYDRTQDVA VLQLRGAGGLPSAAIGGGVAVGEPVVAMGNSGGQGGTPRAVPGRVVALGQTVQASDSLTGAEETLNGLIQ FDAAIQPGDSGGPVVNGLGQVVGMNTAAS Protective antigen Our lead construct, Mtb72F, codes for a 72-kDa polyprotein genetically linked in tandem in the linear order Mtb32(C)-Mtb39-Mtb32(N). All forms of Mtb72F immunization tested resulted in the protection of C57BL/6 mice against aerosol challenge with a virulent strain of M. tuberculosis [Ref354:Skeiky et al., 2004]. MTP Mycobacterium tuberculosis H37Rv 3205113 57117088 Rv3312A AL123456 YP_177957 83332 3700704 3701015 - pilin 4.1 10370.63 103 >NC_000962.3:3700704-3701015 Mycobacterium tuberculosis H37Rv, complete genome GCTATGCGCCGCCACCGGCAGCCGGGGGCGGCGGCGCAGCACCGGGATCGTCAAGCACGGGACCTTCGAG GATGGGTCCGGGGTAGTCGCGGCTGTGGTCGGGGCCGTCGCTGTCGCGGTGGAAGTCGTCATGGCAGGTG TAGGGATCCCAGTTGGGCCCCCATGCGGGGTCGAAAGGCTGCCCCGGGCACCAGTAGTAGTCGGGCACCG GCGCGGTTTGGGCTGCGGACTGCGCGCCGACCCCGAGACCCGCCACACCCGTGGCCAGGATGCACGCCGC CAGCATGAGCGTGCGGCACGCGAACCGGTACA >YP_177957.1 pilin [Mycobacterium tuberculosis H37Rv] MYRFACRTLMLAACILATGVAGLGVGAQSAAQTAPVPDYYWCPGQPFDPAWGPNWDPYTCHDDFHRDSDG PDHSRDYPGPILEGPVLDDPGAAPPPPAAGGGA Protective antigen Nos2 Mus musculus 18126 6754872 RP23-341J22.2 AF427516 NP_035057 10090 78920858 78960123 + nitric oxide synthase 2, inducible 7.76 123528.57 1144 Also known as iNOS; Nos-2; Nos2a; i-NOS; NOS-II >gi|372099099:78920858-78960123 Mus musculus strain C57BL/6J chromosome 11, GRCm38 C57BL/6J TGGCCCCACGGGACACAGTGTCACTGGTTTGAAACTTCTCAGCCACCTTGGTGAAGGGACTGAGCTGTTA GAGACACTTCTGAGGCTCCTCACGCTTGGGTCTTGTTCACTCCACGGAGTAGCCTAGTCAACTGCAAGGT GAGTCTTGTGTTGGGGACAGCTGCTCTTAGGAGTGTCTTCCTGCTTGGGAAAGCCCAGAAAACCAGGCTT TCCCTTCTCCCCCCACCCCACCCCTCTTTTTGTGGCTGGACTGAAGCTGATATGGAAGGTGTAAGAAGCA CACCCACAGAAGGGTCTGCCAGTTGAACAGGGCGTGTGGTCAGATTTCTTCTCTGCAAATGTTTACTGTG GTGATTACACTACAAAGGATAAACTTTCTAGAAAGCTCCGAATAGGCTGGGGGGGGTGGTGTTAATACAT ATAGCTTACTTTACTCATCTTTATGAGATGTGCCCTCTGCCCCACCCAGATCTGATAAGATGGTTATTAT TAATCCCATTTTAGGAGTGAAGCAATGAGTTTCAGTTAACTAACTTGATTTAGATGAGTAACTAGAAAAA CTCAGGGTAGGGTCCAGTTCATCGGGACTCTGAGTTTCACTGTTCCCCTTAGGTGGGTTCTTGTACCCCC CCCCCCCGTATCTTCCAAGTATATCTTTCCTCAGGGAGGGCACTCGCCAAGGAGAAGCCACATACTCTCA GGGCAGTTCCACTTTCTATTTCTTGTCTTTTTCGTAAAGGATATAATCTCAACTCTGGAGAGTTTAATGC TCTTTTATACAAATCATTGACTTCTTCTCAAGTAACAAAGAAGAAGAAGGAACATGTGACCTAGTGTCCT TGGCTCAGGATGCGTGATGACTCCCAAATCAAAGGAAAGGTGGCCAGGACTTGAGTGCTAGCCCCAAGTG CAGGTCCAGAGGGAAGGGTACTAATGACAAGTGCTTGCCCCTCTCTCTGGTCAGCAGGTGGCAGGAAGGG TCAGGCAGATGCACACACTCCTGTATTAGTGACCCCAGCTCCTGCCCACCTTGCTGGAGGCCATGGAGTA ACTCCTGCCAAGAGAAACTGAATTGTCCTAAGGAAGCTTGTGGATGCTTGCTGTCCTTGGTAGGTGGCTA TGTGGCATCCCAAGTTATCCCAAGTTTGGTTTGTTATGTGTCTTTGTAGAACTGGAGTCTGTCCCACAAG ATTTCAACTCCAAATAAAACTGCCGAGTGCAAGCATGGAGAGAGAGCACTTCTGTGCTAGCCTTAGCTGG GCTCTGTCTCCATTCTCTGGGACACTAGCGCCCGCCCGTGAGAAGGCTCTGTTCTGTTAGGCTCTTCTTC AACCTCACCTCACAGCCTCCCTCCCCTTCCTCAGAGAACGGAGAACGTTGGATTTGGAGCAGAAGTGCAA AGTCTCAGACATGGCTTGCCCCTGGAAGTTTCTCTTCAAAGTCAAATCCTACCAAAGTGACCTGAAAGAG GAAAAGGACATTAACAACAACGTGAAGAAAACCCCTTGTGCTGTTCTCAGGTAAGCAACCTCTCTCCCAC TTCCAGCTTTCTCCTCAGGGCTTCCAGGCCCCTGCTGCCTCTCTCTGTTCTGGGCCACCTAAGAGAGTCT GGTCTCCAAGGCCCTGCTTGTAATACTCTACTGGAGTCTGATGCACCTTCCTTGGTGTGGACAAGGAAGG CCCAGGATCCCCATGCTGCTCCTGAGTGAGTCTGTACTCTCTGGAGGAAGAAGGAAGGGGGAAGGGGAAT ATGAGAGAACGGAGACTGGAGAGCCTAGTGGTCAGCTCAGTAGTGACGTCCTGATTGGCCAGGGCATGTG CCTCACCAGTTAGATATTTTGACTATTAGGCAACTGAAGGGTGTCAAGATGGCCCTGTACTATCCTCAGT GACTGTGTGACTTTGAGTAAGTGCACCTGTCTGAGTCTCAGGTCTGCAGTGTGATTTACCTGATAGTCAC AGAAGGGCTTGTCACCATTTAAGAGGCTTCTTTGTCACAGGACTACACCGTGCACACACCTACACTCTTT GAACGTAACCTTCCTGGGGCCCTTAGTTATGATCTCATTTTGCAAGTGAGGCTCTGGAGGGCAAAGTCTG GCTTACATGACTGCTAATGTCAGAGACGAAATCTGAGTTTGGCCTGAGGCATCTTAGAATTGAACTAGTC CTCCAACAATTTATCCGGATGGACTAGGAGAATTCTAGAGAATGCTAGGAAACTACAGCCATAGCCAGGG ACTTGAGGCTAACTTACGTAGAAAGATGATGAGCTGGCTTTCTTTGCAATGTGACTGCACAGAATCACGT CCAAGTCTCAGTCTGTACCTGGCTCAACATCTGCAGGACTACAGTGGACAGCGCCCCCTGGAGGCAGTAA GCAGGAAATCTCTAAATTTCCCTGTCCTGGTGTTGCCAGGTTTAGAAAGCAAGTTTAGGCAGAAGGGAGT AGCTTTGCCACTTCATAATCCTGTGGCATTTGTGACGTCCCTTAGCCTCCAAACCTCTGCCTCCTCACTT GCAGGAAAATAGTCATTATGTTAGGAGTAGAGGAGACAGATGGCTCAGGAGTGACAGGCAGAGTGAGTGG GAAGGGACTGGACCACAGCTGTCAGCAATCCTGGGGCTCAGGTGCCCTCTAGCACTTCAGTCTCAAAATA CCGTGACTTCATCCCGTTTTTTTCTCTTTACCCGGTCCCTACAACCCCTACCTTCTGTCGCCTCAGAATC CAGGTGCCTCCACACCTCTTTCCCTCCAACTTGACTTCATAGAAGTCATGCCCCTGCTCCAAAATCTTGG GTAGCTGCTGGTTACCTGTCATCACACTGAGACAAGAAGGAGGGCACATCCTCTGACAATCCAGAAGGGG ACTGGGTTGTCCTCCCACTTTAGGGAAGAGGAGACAAAAGTCCACCGTGCTTGAGTCATCTGAAGATGGC AGTGGTGAAGCAGGCATTCAAACTGGACAGTCTGGCTTATGGCCACCATCCTGGTAGCTACACTGTAGGT CTCCAATCGAGATGTATCCCAAGGACAAGGACACTTGAGGCTTTCTCACCTGGCACTATACTGCCAGTTT ACATGTCAACAGAGCCCTTCTGCTTCATCAGTGGATTGATGCAACCATTTATTAACTGCATATATGGCCA CACATATTTATAGTGTCCTTAACACTATGTCAAAAACCAGGCAGGAACTGCTTTTATCGTGTCTAAAGAG GTCAGTTTCTAGAAAGTGGAAGAGCTGAGATTTGAGCCTAACTCCTGTCTCTGAGGACTTAGTAAACTCT GGGCTGAGAAAGAGACAGATCCAGTCGCTACAGTCAGAGAGGAAACGGGTGGGGTGGGGGAGGGGGTTGC GCATGGAGAGCAACAGTTTCCTCCCTAGTTGGAGGCAGTCTTGGCAAGGCAAGATGTTAGAGAGGTTTAT GCGTCTCTGAGCCTCCTCCTAAGGGCACCGCCTGGCTGTGGGGTCAGGAGGGCCTAATGAGCTGCAGGTG GCTTTCTTAGAGGAGGGGGCCCAGCCGAGATGGGACTGCCCAGGAAAGGTGGCAAGAGGATGTTCCTGGA AGAGGAAGCCGCGTACAAAGGGGATTTTAGTCTGAAAGCAGTGGGAGCCCGTTATTTATTTTTATTTTAA GACATAATTTTCAAATCTATGCAGACGAAGAGCCTATTCCTGGTGGCTTTCAACTTTGTGTAAACAAGAG ATTCAGAAGTGTGCAGGGTGTGTCCTTGCATGTGTTTTAGAGACAAAGGATAAATGTGGGACCGTCTAGG TGACCCACAGGGAGTTTAGAGCAAAGGGTTTCAGGATGCTTAAAAGTACAAAACAAAACAAACAAAAACA CCCAAAACACTCCTCGCACTGTAATCGGGCTTGGGGATTCATTTGCTGAAATATTAGCCAGAAGGGTCCC TGAGGGCTGTAGCCCTATTTCACCTGCAACAGGGAGAAAGCGCAAAACATTTCCTGGGAGCGCTCTAGTG AAGCAAAGGTAAGAGATGACCCTAGGGCTGTTGTATGGCCTTGAGCAGGATCCTCATTTCTCTGGTCCCC CAGCTTCTGCCGCCATAAAGCAGGGGTAGTAAAGAATTGCCTTCTTAGTTGGATTTCGTTAGGGTGTGAG GACATAACCCGGTGCAGCGGGGAGTCTGGGTCGCGATCCTTACTGTCCCTTCGAGAGGAATACACAAACG AGGCACTCGCGCGCTCCTGCTTGTCCTGGGCCACTAAGAGCTGAGTCCTGCCCGCAGCAGGAGGGCGGGC GCTACGGGGGCGGGCGCTGAGGGGTTTCGCGGCGATAAAGGGACAGCGCCAAAGAGGAGCGCTGGCCCAG CTATGCGCGAGGGGCTGAGCAGCGCTTGCGACGCCGCCCGGCCGCGGCTCTGTCGCTTCCTGCGCAGGAT CTTCGGCCCGAAGGTCGCCAGTCGTGTGGGCCCAGCCAGGTACAGAGCGCGGGACCTGGGGAGGGGCCCG GGTGGCCCCGCTGGGACGCGAGAGAGGCGGGTCAGAGCGCCCACGCCGGCGGCCCTGTAAGGGTTTCTGC ACCAAGGAACTTTTGCGCTGCACTGCTTTGGACAGGTTCTAGCTATAACGATTACTTGGCGTCAAACCTG GAAATCTTCCTGCCTCGGCTTCTCAAATGCTGAGATTACAGGCGTGTGCCACCGCTTAAAGATGTTTTTT TGTTGGGGGGGGGGGGGGAAGCAGTACTGGGAACTGAACTGCATTTAAGCAAGTGCTCTTCCACTGAGCC ACTCTCCCCCTCCCGGCTTGCTGCTCTCCCTCCTTAATGCGCTCTGAACAAACAAGGTTAAGAAGCCAAA GATGATCTTTTACGATAAGACGTTCTCCCTTTCACAAGAGAGGTGGGCTGGCTGGTAGCAGTTCGATCCC AGGACTCAAAACCCTAAGTAAGCCCCTGCTGTGGGGTCTGCACTCACCCCTCAGTACCTAGTTCTGGGAC ACCGTGACAGCAGATGGTTCTATGGACCTGAGCAAGCCACCACAGTTCCCTTTGTCCGAAAGTCCATAAA GGTCGTTCTCCCTGCAATGGGCCAGTCAGGTCTCAGCAAGGCTGTGGGTTTAGAACTGCAGGAATTTGGA GCTAAGGGTCCCGAGCCTTAAAGTGTTCACCTGATATCTGGTACCTAGATTCCCTTTAACAGGTGGCAGA AGAAATCTGCTAGCAGGACATAATAGCTGGGGCAAAGCTTTATTTATCGTTGTGGGGTTTTCCCCTTAAT CTCTATTATAGCAGTACTTGAAAAATTATAAAAGTTCCAATTATAATAAAAACGTCAAAATACTTAAAAG TTAAAAAGAAAGAGATTTAAGTCACCCGTGGCTTATCACATCGTGCTAATCACTGTGAATCTTTGCTGGC ATTTCCCCCAGTTGCTTTCAGGCCTGCTGGAGCCTGCATTCATTCACACACAAGCACACATTGCATGCGT GCACAAAGACTGTCATCACAGCGTCACAGATATCCAGGCTGAGGCATTAGGCACAACCTCAGGCTGTGGA CCCTGAGGCCTGCCTAACTGTGGCTCATGTCAGCAGTGAGACTGCAGACAGCATTTAAAACATTTTGCCG TCTGGGGCAGGTTTACATCCTCACCAGAAGGAGGATCCCCAGAAAGCAAATCTTGGCTTCCGGATTTTTT GGGTTTTTTGTTTTTGTTTTTGTTTTTGTTTTTTTTCCTCCTTCTGGTGAGGTATACCAGTCCAGATCAG AGGAATATGTTAAAGAAAATATTATTCAGATGGGTGTGGTGGCACATGCCTGTAACCCCAACATTCAGAA GGTAACATCAAGAGTTCAAGGACATTGTCAGGCAAATGGTGAGTTCAAAGGCAGTGGAAGGTCATGAAAC ACTGTCACAAGCAAGAAGGAGAGAATTGTTCAATGAGTTTCTTGGTTTGCTTTGGTGGTGGCGGTGGGAA GTTATTGTTTTGCTTTGTTGTTTGAGATAGCCCCTCACCTGAGATAGCCCAGGCTAAGCAAGTCTCCTGC CTCACCTTCTGAATGCTGGGGTTTCAGACACCAGTCAGTGTATGTATCCCTCTTTAGTGATACCGGCTAA CATACCGCAAAGAGGACTTTCCTGAGGACTGTCCCTCAGGTGAGTCCAGGACCCACTGCAGTCCGTAGCA GAGGGAGAGATAGGGCTCCACCCAAACCCAGCAGGGGCAAGGGCAAGTTACAGCCCCAAACCCAGCAGGG GCAAGGGCAAGTTACAGCCCCAAACCCAGCAGGGGCAAGGGCAAGTTACAGCCCCAAACCCAGCAGGGGC AAGGGCAAGTTACAGCCAAAGGTAAAGAAGAGCCTCACTGAACTGACCTAACAATCAAACCAGGGATCGA GTTATTACCAAAACACAAAGCAGGGCCATAGCTAAGCTGGAGTTTGGGGGGTTTGTTTTGTTTTGTTTTA GATTTATTTATGTGTATGGATGTTTTGTCTGACTGTATGGGTGTATAGCCATGGCTGCCCTGGAACTCTG TAGACCAGGCTAGCCTCGAACATAGAGCTCAGCCTGCCTCTGCCTCTTAAGTGCTGAGATTAAAGGGACC CACCACCATAGCTGGCTATATATATATATATATATATATACACACACACACACACATATATACATATATA TATATATATGCACACACACATATATATACACACACACACATATATATACACATATATATGTATATATGTG TGTGTTTCTTTTTATATTATATATATATAATTTTTATAAAATTAAATTTATTTTAATTTAATTTTTATCT TATTTTCAAAACAGGTAGCATATAGCCCAGGCTAGCTTTGAATTCCCTACTTAGCTGAGGATGGATGACC TTGAACTCCTGATCCCCCTGCCTCAACTTTCCAGGTGTTAGAATCATAAGTGTGTGCCTTCTGTACCTGC CTTTGTTCCTTTCACTCTTTACAAGCACGGGGCTTGAACCTGGAATCTCTGCACTCAAGAAGCCCTGGCA AGCGGATGGAGGCCAGTGCGGTGCACAGAGTAAACAAACGAAATCAAGGGTTTGGGGTGCTTTGTAGGTT CTCTTGGAGCCCAAACTGGCTTTGAAGTTTCTTTGTAGTTGAGGTTGGCCTCAAACTCCTGACCTTTCTG TCACAACCTGCCAGATGCTAGGATTCCAATTTGTAAAATGCCCAGCTTGAACTAAGAGATGAGCTGGGCC TGGTGATGCATGCTTTTAATCCCAGCACTCAGGAGCCACAGGCAGGTGGATCTCTGTGAGTTCAGGGCCA GTCTGGTCTACCTAGAAAGTCCCACATCTTAAACATACAATACTCACCAAAATGAAAACCGAAAATACAT AAGATAAAGTTTGCTAAGCCAAGTAGGTGGTGAGTGATGGACCCTGCTGGCCTGGGAGGGCTTTTGCACT TGGCCCTCTGAGGCAGGCATCTCCCCTGAACTTGTGTGTGTGCCTCTGTCTGAGTAGTTTTGGCTGGCTC CCCTCATATCATTTTGGCTTTGAGTGGTCAAAGCAAAGATGAAAAGGGAAGTGGGGACAAGATTCTCTAA GACTGGCTCCCTCATCCTTCCTGATTGGAAGCCGGGAACAGGTCATTCCCACCTCTAGCCCTTGGTACCC CGTCTGCCAGATCGGGACTCACAGAGGCCACTGGAGATCCCCTCCGTGGCCAGACTCCATCTCACAATGA CTGTTTCCTTTCGCAGCCCAACAATACAAGATGACCCTAAGAGTCACCAAAATGGCTCCCCGCAGCTCCT CACTGGGACAGCACAGGTCAGTGGTTTGGTCATACAGTATGGGAAGCCCTTCATTCTTTTCCTTGGTAGA TTCAGGCTAAACAGCCCTTTGGAAGCTGGGCAGGAAGGCACAGGAAGCCAAGGGCTGGGGAATAAGTGGA GCACCCCAGACTTAGGGTACAGAGGGAGCCAAGGAGGTTGCAGACAGTAGGACAGTCTTGATGCTCATCA ATAGAGGTCCCCAGGGTGCGCTGGTACCTAAGTAACTTGCCTGGTGGTTGACAGAATGGGTCGGGGTAGT GCCATTAGGGCTCTGTTTCTGTCAAGAGATAGCATGACCAAGGTAATTCTTTTAAAGGACTACATTTAAG TGGGGCTGGCTTACAGGTTCAGAGGTTCAGTCCATTATCATCAAGGTGGGAACATGGCAGCATCCAGGCA GGCATGGTGCAGGAAGAGCTGAGAGTTCTACATCTTGTTCTGAAAGCAAACTGGAGAAAACTGTCTCTTC CAGGCAGCTAGGAGAAGGGTCTCAAAGCCCACACCCAAAGTGACACACTTCCTCCAACAAGGCCACACCT ATAAGCAGTATCATTCCCTATGGGCCAAGCATATTCAAACCACCACAGGTAGTTTAGGAGCATTCTCTAA ATCAGGGAAGGACGGTTTTCCAGAATGATTTCAGATTCAGCTTCTGAGTCCCTAGTCTCAGCCCCTGGCT TCTTCCATCTTCCTGAAGCCCCTTACATCTTAAGGACTGTAGAGGGGTGGCAGAGAGATGGAGCCGGGAA AGGTGTCCCTGGGGAAGGGAGACAGTGGAGTATCTGTTGACTTCCCAGCTCTGCCCTTGGCAGTCCCAGC CACAGCTTCGGCCCTCCCATCAGACTTGAATTCCCCTTATGAGCCAGCACTGGCCAGGCCTTGGTAAGGA CAGAAGTCTGGCCTTGGTCCAGCCACCTGCAGTGCTCAGCTGGCACTAGCCCAACTTCTGGGAAGGGTGG AGGACCGAGGACTGGCCCCTTGCCTTGTGTGCTCACGCAGAGCTCCGTCCCTCATCCAAGCAGAGTACAG AGGCTCAGGGGACACTTGTATGGTTGTTGAGGTTATAGACATTTAGAAAACTCTTGATGTTTACCCATTT TAGAATGTTCCAGAATCCCTGGACAAGCTGCATGTGACATCGACCCGTCCACAGTATGTGAGGATCAAAA ACTGGGGCAGTGGAGAGATTTTGCATGACACTCTTCACCACAAGGCCACATCGGTAAGAAGTCTGGGGGC TGGGGAACACCACCTTTGTCCCAACTAGGCCATCATAAGGGCCCTTTGTGGGTCTATCACATATTTCCTG GTCTTATTAAACAGCCATCTTCTCAGACAAAAGATGTTGGGAAGTCTGTCTTTATAAATTATAGCAAGAA GAAGGAGAGGTTAACTACTGGGTTAGACAGCAGATAACCTAAGATGTCAGACAAAAAGCTACAGAAACCA GTAGAGTATCTGAGAATTGTCTATTAGAATTGCCCAAAGCTGGACTGAACTGGCAGAGGAAGCTTCCTGA CTCTTTAAGGACAGCAAGGTAAGCCAGGCAGTGGTGGCAAATGCCTTTAACCAGCACTTGGGAGGCAGAG GCAGGCAGATCTCTGAGTTCGAGGCCAGCCTGGTTTACAGAGTGAGTTCCAGGACAGCCAGGGCTATACA GAGAAACCCTGTCTCAGGAAAAAAAAAAAAAAAAAAGACAGTGAGGTAACAGTGGTTTAAGCAGCCAGTG AATGAAACTAATCACCCTTTCAGGTTCCTTTCTACCTTTAAGTACCCTACTCTGACTTTGGAATTCATGC TATGAGGCTGAGCAGTGCACCAGTGCCCACCTTTAATTCCTTTGATGGAGACAAGCAGATGTCCATGAAT CCTAGACCAGCCTGGGCTACATAGTGAGTTCAGGGACAAGCCAGAGCTACACTGCGAGACCTTGTCTCAG AACAACAATAATAACAACAAAACATAAAATAAGCCAGGTGTGGTATACACCTTTAATTTCAGGAGGCAGC AGAAGAGGCAAGTGGAACTCTGTGAGTTCGAGGCCAGTCTGGTCTACGAAGGAGTCCAGGACAGCCAGGG TTCTGAGAAACCTTGTCTCAAAACACCAAAACCAAAAAATAAAAAAATGAAAAGCCCATAAAACAAAACA AAGGGGGGAGAGAGAGAGGGAGGGAGGGAGGGAGAGAGAGAGAGAGAGAGGGAGAGAGAGAGAGAGAGAG AGAGAGAGAGAGAGAGAGAGAGAATAATTCATACTGTGGTTATAAAGTGGGAAAGAATCTTTGAAGGCAG GATGATTTGCTGAACCCCCAGAAACTGTGTCTATAATGCCAGCTTGGAAGATAGAGGCAGGAGGATTGGA AGCTCACAGTGGCCTGCTATGTAGTGAGTTTGAGACCAGCCTGAGTCACAGATGATCTTGTCTCAAAACC CAACAGCAAACCTCAGCAACAAAGCAAAACAGAGAGACGCCACAGTTGACATGTGGACATCCATCTTACT GGCATTTCTTTCCAAGCCTCCCCTGACTTTGGAATAACCATTCTCTCACTCAGCTTCTCTGGGCTGACAC TGGAGCCCTGTTCCCTGAGGAGCTAGCCGGGTCTCTTTAGAAAGGAAAGCAGACCCCCTAGGGAAAGATA TCCCCTAGGAGGAAGCAGTAGAGTTTTCTCACAGGCTGTCACTGTATCTTTAGCTTACTGGAGAACAGAG AGAAGCCAGCTCACACCCACCTAGTCTCAGGAAAGGAGAGGGGAGCTAGGCTTGGAGAAAGCTTGGTTGA CTCCACGGAGAAGGAGCCTGCATGGGGTCATTGTGAGCATACCGGCCAGAGAATGGCCAGGCTTATGACT GGTTTGGAGCATGGGGGGTCTCGGTGGGATGAAGTGGGGAATAGGGGAATAGGGGCCCTACTCAGCCCAT TTCCTTTTCCAGGATTTCACTTGCAAGTCCAAGTCTTGCTTGGGGTCCATCATGAACCCCAAGAGTTTGA CCAGAGGACCCAGAGACAAGCCTACCCCTCTGGAGGAGCTCCTGCCTCATGCCATTGAGTTCATCAACCA GTATTATGGCTCCTTTAAAGAGTAAGTCTGGCTTTCGTCAGGGTGACATCCCCTCTGCCCTACTCCCTGG GGCTGACCTGTTTCCTACTGTTCCACTCTGATCGGTGAACTCTGCAGTCTCCAGACTTGATGGGGCTAAA GTAGGCTCAGGCACTGCAAAAATGTCTGAAAGTTTGGAGGAGAGTGTAACCGGTTTTGGATTCCGGCGGG CCACCAGGTCACTGCTGAGTCCTGGAGAAATCCATTGGGTCTCAACCTCCTCATCTGTAAAGTGGGCATC TTATAGCATGACTCACTAGACAGCCATGAGATGTGTACAAATTTAGAACAGTGTTCTAGCCTAGGGACTA TAATACAGTGACTTGGAATGAATCCTTTTATCCTGAAATAGAAGGACATTTAGAGACTGTCTGGTCAGGC CTCTCCTCCTACAGATGTGAGCAAGATTCCCAGAGACAGCACTGGCATCAGCCTAGTCTAGCTAGAGTCC GGGAGACCTGACTTGCTGGGGTGCTACATCTCTGGAGTGCTCTCATTTTATGCCATAATGCTTCCTTGAG TCCAGTGTCCAGACCCCTTTATTCTTTGGGGTTTTGTTGTTGTTGTTTTGTTTTGTTTTGTTTTGTTTTT TGTTTTATTTATTTATTTGGTTGGTTGGTTGCAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGC TGGCCTCGAACTCAGAAATCCGCCTGCCTTTGTCTCCCAAGTGCTGGGATTAAAGGTGTGAGCCACCACT GCCCGGAGAGACCCTTTATTCTTAAGCCCACTAGTCAGTCACTCCAAGCACCAAGTATTGTCCAGCATGA AAGGCAATTGTTTTTCATTGCCAGTTGCCAATGTTGTTGTCAATGCAGTTGCAATCATGGCATGGCTGTG TGGCAACTCTTGGTAAGAGTTGGGATCCAAGGAGCCATCTTCTGAGGTTCAAGCCTTGGTTTTCTTGCCT CAAAATATCTATTATAAAATACCCACCTTGTAGGAGTTTATATGAAGATTAAATGAATTAGAGGCTTCAG AACAGTGCTGGCCTGTAATGAGTGCAACCGAGATCTTGGTTACCTCGTACGTACTCAAGCTTTTAAACAT CTGTTTACTTGGTGGAGAATGGGGTACCTGAGCCACAGCAAGTTTGTGAAGGTCAGACAAGCTGTGGGGG TTGGGCATCTCCATCCACAGTGCGGACTCCTGGGATTGACTGAGGTCATCAGACGTGGTATCAAATGTCT TTACCTGCTCAGCCCCTCATTGGCCCCTTTACCCTCTGGCCCCTCATTGTTTTGGAGACAGCATCTCATG AAATAATGAAGGCTGGCTTCTGCCCACAATTGTTCTTGAGAGTGCTGGGGTGACAGGTACAGGGGATGCT GTCTAATTATAGAACATTGTTGACTGACTGATTTACCTGTCCAGTTGTAAGAATGACTGTCAGAGACAGC GAATGTCTACTCTGGCAGCTGGGGACGGTGTGGCAGTGTCATTTTGTGGCACAGCCTCACAGAGCAGTTC CACCTGGGGCGTGAGACACAGAAGGGTATCTGGTACCCAGAGCAGAAGTTTCCATTCTTGGGGAATCCCT GTTCCACCTCTGTACAGCCCTGGAGTGCCAGAGCCGGGAGGGCGCTTGACAAGAGGTCTCTATCGTCTAG AGTAGTGCTTCCCAGCCTTACCTGGGAGCTGGCAGAACAGTGATGACTGGTCCCCCCTGAGCCAAGGTCA ACCATCTTGGGAAAGGCTGTCTGTAGATTTGTGAAGGGTGGAGGCCTGTGGGGGCACTGTCTTTCCTGTA AGCATGTATCCGGGGCTTTGTGGCATTGAACTTCAGTTTCCCAGATCTTCCCACTCTGTTGTTAACAAAT GGCTGTTTGGGGGGTCTGAGATTCTAGATAGGCTTCATCTGGCCATTTTTTTTAATTTGGCACCAAGCAC ATGAAAAACTCTGAGTTGCTGGGTTAACTCTGAGAGCCAGGTGTGGGCAACACAGCTCTCTATAGATAAA TAGGAAGATCTTAGGAGGACTCCAAGTTCAAGGATTGCGTGGGTGACAGGGTGAATACAAGCCAGCCTGA GCAACATAGAGGGAACATGTCTCAAAATAAACGGTTCAAAAAACAAGTGCTGGGGCCACAGCTGAGCATG TAGCTCAGTGGTAGGATAAGCCTGGTTTGGCGCCCTGCTTTTCTTTTGAGGCAGGTACTCACTATGTTGC CCAGGCTGGCCTGAGTTTACCTAATTTACACAAACTCATTTGGAAATTACAAGTGGGTGTGGAGAGACGA AGCCTGCCTATGGTTTGCTCCACGTGCCGAGTCCAGAAGGCCAGAGAAACAACTGTCTCCCTCCTGACAC TCCTGTGAAAGACAGAGGCTCCTGTTTCTTCTTGCTACCATAGTTACTGCGCTTTTCATTACAACAAGAG GATGGCTTTGCTCAGGACCTGTCCACTCCTTAGAATACAACGGGAGAGGCCAAAACTCTTCCAAAGAAAT CAGGCCCACTCCTGGGTAAATGACACCAAGTTTTCCTCTTTCTCCTTGAAGCGGTGTTGCCAGACCACAG ACACTGCTGTTTTCGTGTTTGGGTAGAGTGGGGTGTATTTCTAGAATCTTCCTCTGACGACTAAGGACAC TCACCTCACCCAGAAGTTCCCCCTCACAGGGATCACTAGAGACAAACATCGCTGAATGTTTTGTCTTCAA GTCTTTCTGTAGTCAAAAAGGAGTTGCCAAGACCCTCCGCCTGCCCCGAAGGCTGTTCATTCCAAGTCCT CCTGCTGACCTGAATCCCAAGTTCATTCTTTAAATAATTTTGTTTGTTGCCATTTCGAAAGCCTCTGGTG GTGCTGGTTGGAAATCTCAAGGTTGCTGTGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT TTCAAAGTCTTTGCGACCAGGCTCCTCTCCCTTGGAATTATTTTGAAGTATGCTTAAATCAGTGAGTGTA GCTCTGTTGGGTGGGCTCTAGGGTCCTTCAAGTTGAGTCCTGACCTCAGGTCTTGTCCGGGCCTGCCTTG AGTTTTGAGGAGCCCTCGAGGCTTGCTTCTCCCCTTGGGTGGAAGGTGGGAGGTCCTATCCACGACAGAC AGTTTGGAGCCTGGGGCTGGCAGTGAGACCACCTGGAACTATGTCCTCTCTGTTACTAGGCTTGTGTTCT TGAGCCTGGGACTGTATACTCTAGAGTCTTAAGACTGCTGAGCAAACACAGCAGTGCCTCCTAAGTGACT TGTGGTGGCATGTAGCTGCACTCTCAGGTATTTGAGAGGGGGAGGCCAGAGGGTTGGAGGTTCTAGGCTA GACAAGACCTTGTTATGACTATAATAATCAGCAGGGTCCAGCGTAGGAACATAAGTGTGGTTTTTCTCCC AACAGGACTCAAGATGGAGATGGCAGAGTATTAAAAATCGGCCCCTAGTAAGCGAAGAGTGGGTCACAAA GCCAGCCCTACCGAGGACAATGTCCCAAGGGGCACAATTTGCCCTTCTAGTGTCCAAAATCAGTTCTTGC TTTCTGGGGATTGAATTAATCTCCATGTAAGGTCTTGCTATTGTTGGTCTTCCTGACAATTTCCCGTCCC ACAGGGCAAAAATAGAGGAACATCTGGCCAGGCTGGAAGCTGTAACAAAGGAAATAGAAACAACAGGAAC CTACCAGCTCACTCTGGATGAGCTCATCTTTGCCACCAAGATGGCCTGGAGGAATGCCCCTCGCTGCATC GGCAGGATCCAGTGGTCCAACCTGCAGGTGGGTCTTACCGGCAGAGCAGAAGGAGGAGCAGGGGTGGGCA GGCCCTGAGACAGAGCAAGTGGCCAGGGCTCTGCAGACTACAGCTAAACCCTGAGGTGAGGCATCCCAGA CCCCCTTCTAGGAGCCTAGAATCTTATTTTAGCACCAGGGTCTCTAGATGACCCACCCTATATAAAATGT TGGGGACCACATGCCATCATGAGACAACCAGCAACCTTGGATGTCTTATTCCTGAGGCCTTGGTGCTACA AATGTTTGCCTCCAGTGTTTGCGATCCTGGCCCTAGAGAGCATTTTATTTATCGTGGCCTCCTAGGCCAT CGTCTCCTTGGAGGGTCATTTGGGCAGGGGTGGGGGGTTGGGTCCTATTGAGTGCACTTGGACTGAGAAA ATCCAGGAGTCTCAATGGTGGTGCACTCCCATGGCCAAGATCCAGGCTAGGGCTGTGGGGATCCTGCCTT TTGGTTCTTTTCTTCTTTTAAGATTTATTTATTTATTTTATGTATATGAGTACACCATTGCTCTCTTCAG ACACACCAGAAGAGGGCATCAGATCTCATTACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGAATTGA ACTCAGGACCTCTGGAAGAGCAGTCAGTACTATTAACCGCTGAATCATCTCTCCAGCCCCTTTTGGTTCT TTAAAGCCAGACACTTTTGGTAATTTGGTTTTACTGGGGTCTTCTGAATGGGATTCTCTGATCCCTTGTC TCAAGAGTAGGGCAGCCTAGAGAAGGGCACAGGGGATGCAGAGAGGAGGGTGTGACCCTGTGAGAGAAAT GCCCTCTGTCCCCAAGGACTTTGAATCAGCCCCAAGCCTCTGTCCTCACAGTCTTCCATGGTGTGCCCTG TGCAGGTCTTTGACGCTCGGAACTGTAGCACAGCACAGGAAATGTTTCAGCACATCTGCAGACACATACT TTATGCCACCAACAATGGCAACATCAGGTGAGGCACCTTTCCCAGCTGTGCTGGGCCCAACTGACTACTC ACTCTCAGGAAGAAGATGCTGGAGAGCACAGGAGAGGGGCCTCTGGCTCCAGGAGAAACTAAGGAGAGAG AAAAGAATCCAAGACTTAGCTTTTCCAGGACATGAAATGGCAGGGCAGCCTCCTCCCCATTAGTCTCTGT TATAGCCCAGCTCTTCCCCGACCCCTTTCTAAGTTAGAACCTCAGGGCTGTTACATCTTTAGGAAATTAC TTTGAGCAGGCCATGGTGACACATGCCTGTTATCCAAGCACTTGGAAAGGTGGAAGCAGAAGGATCAAGG GTTCAAAGCCATCCTTGGCTACATAGCACGTTCAAGGCTAGCCTGGGCTCCATGTGTTCCTGCCTTAAAA ACAAAATAAAACCAAAACAAAGAAAACCATTTTCTTAGTTGGGCATGGTGGTACATAACAGCAATTTCAG CAGTGGAAGGCTGAGGCAGGAGGATTACCATGAGTTCCAGGCCAGTCTGGGTCACATTGACAGACCTTGT CTTGTTTTTAAAAAGAATCTACTCAGAAGGCAGAGGCGGGTGGATCTCTGTGAGTTCAAGGCTAGCCTAG TCTATATAGTGCTCAGTCTGTCATTCACTGTCATGCAAAAAACACGCACCTAATGGGGATTCAGCACAGG ACTAGGCCAGGGGCCAGGGAAAAGGAGGTGACAGGGTGGGATACAGAGGCGACTGAGACCCTAGAATGCA GCTCCATCCAGCTGCAAACCTATGGTGCACAGCCACCAAGGGTGAGAATAAAAGGATCCAGCAGCGAAAG CCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAAGGGACATGAGGCTTCC CAGAGTGGTGGCCCCTTGTTGGTCTTGATAGTTAGGGAGAAGCTGGCAGGAGGAGGAAGAGGAGGGTGAT ATCAGGGTGATTGGGATGCAGGGAAGAGTGGAGGGAAGTTGGCTGGGAACCCCTGACCCACTCACCCCAA CCTGGGACAGTTGATAAACTACTCTGTCCCTGTTCTGGGGAAGGTCGGCCATCACTGTGTTCCCCCAGCG GAGTGACGGCAAACATGACTTCAGGCTCTGGAATTCACAGCTCATCCGGTACGCTGGCTACCAGATGCCC GATGGCACCATCAGAGGGGATGCTGCCACCTTGGAGTTCACCCAGGTACTGACTCAGCCTCTCCTAGATC CCTGTGGGCAGGGGACCCTGAGCAAATGTGGGGCCTGGCCCTGCAGACTTACTGGGCCCCTCTCTCCCTC AGTTGTGCATCGACCTAGGCTGGAAGCCCCGCTATGGCCGCTTTGATGTGCTGCCTCTGGTCTTGCAAGC TGATGGTCAAGATCCAGAGGTCTTTGAAATCCCTCCTGATCTTGTGTTGGAGGTGACCATGGAGCATCCC AAGTAAGTCACCCAGGGTACGGGTTATATGCATGCAAGCCCATCTCAGGCAGCACCCACAGGACAGATGC TGCAAGGCCTCTTTGCCCTCTGCAGCAGCTCTGGCCTTTCCTCTCCAGCCTCGCTTTATGTGGAACTGCT GATGGTTACCAAGGGAACAGCAGCCTAGCAAACAGGGTCCTGGGAGAGTGGGATGCAGTGACTTAAGGAA AGCCCCTGAGCTTCTGTCTGAGCAGAACCAGGCTTTTTTCTCACCTCCCAACAACTTCTTTAATTTTTTT CTTTTATTTATTTAGTGTGTGTATTTGAATGTGTGTGCTTGTGTGTGTACCTGTATGTGACTGTATGTGT GCATAGGTGTGCATGTTTGTGTGTGTGTGTATATGATATGTGAGGTATATGTGGTATGTTTATAATCATG TGTATGTTGTTTGTATGTATGGTGTGTGGTGTATGTTTATGTTTGTAAGTGTGGTGTGCTTGTATGTTTC TGTGTGTGATGTGTATGTGTGGTTATGTGTGTGTGTGTGTGTATGGTGTGTGTGTGTGTGCTCAGAGGTC ACTTGCAGGAGTCAGTTGATTTGCTCCTACCATATGGGTCCTAGGGACTGAACTCAGCTTATCTATCAGA CATGGTAGCTAGTGCCCTTACCTGCTGAGCCACCTCAATGGCCTCCTCCCAGCTTTCTTAGGAAAAGGAC AACCCTCCCAGACAGAGGAAGTGTAGGCTCACAAGGACTTTAAGAGTGCAGCTGGGCCAGGCAGTGGTGG CGCACGCCTTTAATCCCAGCACTTGGGAGGCAGAGGCAGGCAGATTTCTGAGTTCGAGGCCAGCCTGGTC TACAGAGCGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTTGGAAAACAAACAAACAAACA AACAAACAAACACGAGTGCAGCTGGTTGAACCTCTGCAAAATGCCTCCATATGTGGAGGTTTACCAGCTA GCAGGTACTGACAGCTCCTGTCCTTTGAACACACTGAACTCAGTCCTGCCTCAGGACCTCTGTCCTGCTT CCCTGCCTGCTATGCCCTTTCCTCGGAATTCTGCAAGGCTCCAGTTTTCCCATCAAAAGGTATTCACTAT GACATCACTGCACCTCGGAGAAGTCTCTCCTGATCTCCTATCTTCAACCCCACACCACACTTCACTTCGT TTCTCATTTTCTCACTGGTGTATTTATTTACCCCTCATAGTCTATAGTCAAACGGTCTATAGTCTGGATT CTGGGCTGCAACTTTGGGAATAGAATCTTACTTTTTATCTATAAAGTGCAGGGCACGTAGTAGGTGCTTA ATTATAGATATTTAGAATGTCTAACTATACAATGAATGATACTATGTTTGCAATAATCCCATGAGTTTGT TGTTATCGACTCTGTGTCTATGATGGAGATACTGAGGCTCCGAGGGGCTAAAAGGACCCTGGTCAGTGCT TGAACCCCAGCTGTCTGGGTGCCAGAGTACTTTGCTGCTACATTATGTTGGAGTCTCAGCATTTAGTCCG ATATACACCTGACTTATTAGCATGAATGAGTGAGTAAAGAGATGTCTTCTACAATAAATGACCCTAAGTG TTCCCTGTTATCTCTCGTGTGTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGACT GAGATCATCTCTTCAAAAGGACTCTCTAGAGCTCGGGCTATGGCTCAGCTGAGCATGATTGCTTAGCATG CTTGAAGCCCTAGCTTTGCTCCCCAGCAGTGCATAAACTGGGTGTCGGTGATACATGCCTATAATTCAGG CCCTTGGGAGATAGAATCAGGAGGATCAAAAGTTCAAGGCCATCCTCAGGTACAGAAAATCAAGACCAGT CTTGGAAATGAGACCCTGTCTCAAAACAACAAAGAAAAAGAAAAGGAAGGATTTCTTTGGTTGATCCTTT TACTGAAGTTGATTTTTTTTGATTGGGGAGAGAGGGCAATAAAGATGGCCCTAAGTGCAGCTGGCTCTGC CTCCCTGCCAGGTACGAGTGGTTCCAGGAGCTCGGGTTGAAGTGGTATGCACTGCCTGCCGTGGCCAACA TGCTACTGGAGGTGGGTGGCCTCGAATTCCCAGCCTGCCCCTTCAATGGTTGGTACATGGGCACCGAGAT TGGAGTTCGAGACTTCTGTGACACACAGCGCTACAACATCCTGGAGGTGATGAGGGTTACCAAGCTGGGA CAGCTGGAGGATCAGGATGGGGGACCCACTCCACCCAAACTGCCTCACCCTCATCACTTGGCTTCTATAG GAAGTGGGCCGAAGGATGGGCCTGGAGACCCACACACTGGCCTCCCTCTGGAAAGACCGGGCTGTCACGG AGATCAATGTGGCTGTGCTCCATAGTTTCCAGGTATGCCAGGTATCTTGGGGGTTGGTAGAGGTTTTGGC AAGAAGGTCACTTGATCCAGGTACTTGACTGGCACTTGATGTCCCCTTTACAACATCATTCATTCATGTG TGCATTCATTCATGCTTGCTGGGTGCTGAGACCCAGCATTATTGCTGGTCTAGCTCTGGGCAACTCCTGT CCTCCCAAGGCTGCCTGCTCCCTGCTCCTGCACTTGAGCACTGTAGGCAGCTTGGCTTCCTTTGCTCCAG GACATTGCTTAGAGACCCCTCCTCCCGGCTGCATCTCCAGGGCCTTCCTCCCTGCCCCATCATTCCCACT GTGCACCAACAGTTCCTGCCAACCTCTGGCTCCTTCTTCTTCTCCTGCTCCTGTTACAGCCAATGAGACC CATCTCCTCACACAGCCTCTTCCTCCGCCACTTGCAGCCTCCTTTCTGACTCCCTGGGACATCTGAACTT CTTGCAGGGATCAAAGACTCAGGAAAAAAAGGGATCCTAACACAGGACCATAGCTCTCCTAAGCACATGC CATGGTGTGTGTAAAGGTCAGAGGACAGCTAACAGGACCCCGTTCTCTGCTTTCAGTATATGGGTTCTAG GGATCAAACCCAACTTGACTGGCTTGGCAGCAAGCACTTTTACTCCTGAGCCATCTCGCTGGCCCCCTTA CTTTTCCTCCCACCCCGTTTCTTCTTCTTTTAAATCACCCCACGACCAGGTCCTTCCCTCCTTGAAGGCT TCAGTCGTTCATGTCCTTAAAGAACCTCTCCTGGACCAGGCCACTTTGGGCAGTGCTCCCCCGTCCCCCA CACTGTCTTCTTTCCTTCCAACTCCCTTAACTTGACTTTGTTGAAGTGGTTCCCAGCACCAGGTCGTAGG TTGACGACCCCAATCTTTGTTGTTTCTTTCCTCCGCTGGAAATGTGAGCTGCGCAGTGCTCAGTGCTCCC TGTGGCCGGGATTGTGACTGAGTTCACTGCTACACCTCTAGTCTAGCTCATGATGGAGGATTGGGAATTA TGGAATGGATGGACAAGTGGAGGTGAGCCGCCCCCGCCCCCCCCACACACACACATGAGCAGGGGCCTTG TGTGCTAGGTGGACATGGTAGCTGGTTCCTGTGTGAACTGAGCCTTCTCTCCTCAGCTTTGCTTCTGTGG TTCTGTTTCCTGTGGAGCCTGGATATGTGAAGAGGTGGGGAGTCCAGTCAGGGTCATTCATTCTTTCAAC GGGGATTTAACAAATGTTTGCCCCATGTGTGCCATGCATTAGCTATGGGTTCATACAATGTCCTCCAGGA CTGGGACACTCTCTGGAAGATTAATTTTGTGCTCACTGGAGTAAAGGGTGGTGAGCTAACACAAAGAAGG AAGAGCCCCACCCCCAGCCCCTGGGGCAGGCCCAGGCAGGAAGGACCACCCCTGGTTCATACATCCTAAC CCCCAGGGACTCCCACCGCCAACCCTGACTCAAGCAGGATGCAGATGTGTCCCAGCAAATGCACAGAGAC ATAGTCACCCCCTGATGATGTCCCTCTACTGCCCAAGTCTCCAGGCCATTCTTGTCACCAACTTCTAGGA CACCCTTCAAAAAGAGATGGCCTTTTTCCCTTATAAAGTGAGGGCAGTAGACAGGTGTGATGGAGCACTA CTGTAAGTCTAGCATTCAGGAGGCGGATGCAAGAGGATGGTGAGTTCAAGGCCAGCCTGGGCAACTCCAT GAGTTATTTCTCAAAGAGGGAAACAAAACAAAAAATGGGAATAGCGCTTCCCTCGATGAGTCATTGCTAA GATTAACTGAACGTGAGACACCTTTGGAGCTCTCTTGTTCCCGAGGGGTGCTCAGCATACGTGGTTGGTC CATCTATGCTTGTCGGAGTTATCATCCTCTCCTGACATCCAAGGGCACTGTATATGTGCACAGATAGTAC CTGGCTTACGAAGGCTCCACTTGGGAATTTTTTACTTTACAAGGAAGGATATGAAGGCACCGTGAATCTA GAGCAACTCTACTTGGAGTTTTTAAAATTCTTTTTTTTTTTCATTTTATTTTATGTGTATGGATGTTTTG CCTGCACCACATGTGTGTAGTACCTGGCCAGAAGAGGGCATTATATCCTCTAGGACTGGAGTTAGAGGAT TGTGAGCTGCCATGTGGTTGCTGGGAATCAAACCAAGATCTTCTGGAAGAACAGTCAGTGCTCTTAACCA CTGAGCCATCCTTCCAGCCCCCTACTTGGCTTTGTTGTTGTTTGAGACCTTGACTTCTCTCTAAAACTCT TTTTTTTTTTGGTTTTTTATTTTGAGACAAGGTTACCCAGGCTGATCTTCAGTCACTCTGTTGTCTAGGC AGGCCTTAAACTCAAGGCAGCCTCCAGAGTCTCTGTGCTGATGGGCCTGTATGGTTAACCGCATAAGGAA TTTTAATTTCGATCTTTCCTGGGCTAACGATGATGGCCGGATGCTCTTTTGAAGCTGGACAGCAGCAGCA AGCTACAGCCTCAGCCAGCTATGGCAGCTACAAGGACTAGAAGCTGACACTATAGTGTGTTGTACAGTCA AGCTACGATGGTTGGGAGGCAAAGTGCTTTATCAAAAAGACTTGAGATGGAGTCTCATGTACCCTAGGCT GGCCTCACACCGGCTATGTAACCTCTGATCTTTTTGTCTACCTCCGTAGTGCTGGGATGACAGGTGTGAG CTACCACATCTGAGTCATTTGTTTGTCTTTTTTTTAATGTAGTACTGGGCATTGAACCTCCAGGTCTTGG TGCATGCTAGACCAGCAGGCTATGTTAGCTGAGCTACACCCCAGTCCCCATTCGATGTGTTCTTGACTGA GGATACTTTCATCTTGTGATGGGCCCATCAGCATGGAGCCCTATCATGAGTCAAGCAGCAGCTGTATATG AGTTTTAAAGTGTGTGGTTTGACTCATGAGCCTGACATCAGGTGGCAGCTGCCAACCTTGCCTCATCCTT CCCCAGGAATATCCCTTCCTTGGCCAACTTGAAACTATTTCTTAAGACTCTTTTCAGCTGTCCTGACTCC CACCTCTCCTCTAGACCTATCCCAGACACTAGGGTTTCCCCCTCTGTCCCCTCTCTGTCCCCTCTCTGTC CCTTCCAAGCAGCCTTTGCTTGCCTCTGTCACAAATCCCACTAGGCCTCATGAAAATAGCTGATTTCCTT CAGCCTTTCCAAGAGCCTTGCTGTTTGCTGGCAATACATGGAGACTGTCTCTCCTCTGCCCCATGGTCTT TCTCAGTACCTGACAGATGAACAGGAGCAGATAAGACCCTTTGAGAGCACAGGATGCTTCAGTGTTGGTT CTCTCCTTCCACTGTGTGAATTTCTGAGATGAAACTCAGGTTGGGCTTGGTGACCAACGCCCTTACCTAC TGAGCCCTCTCCCCAGTTGTAAAGAAATTTCTCATGGGTGAATGGATCTTCAGACTTTGATGGGGCCGCT ACTTTTACTGGATCATCCCAGGCTAGTTTAGGCCTCTAGTTTGTAAGATGTGAATGGCTTGTCTTCCCTA CACTCCGGTCCCCACCGTCCCCACAAACCACTCTGGTGGTTTTGTGTTTGCTTGTTTTGGAGACAGAATC TCTTGGAGCCCAGGCTGGCCTCAAACTCCCTACGTAGCTGAGGATGACCTAGAACTCCCAACCCTCATCC TGTACACCAGAGTGATGAGATTATAGTTGTCTAGTGCTTCTTGTATGCCAGGCAAGCACTCCACCAAGTG AGCCTCCTTTAAGTTTGTATTTTCTTTACCTGAAACAAGATGGTGCAGTGTGAGCTGCCCACACCTGAGG CTCTCTGTCTGGTGGCGATGCATGGAGACCTGTGCATCGAATTTCTCGATGCCGCTTTTAGGGTAGTGGT TCTCAACCTTCCTAATGCTGGGACCCTTTAATACACTTCCTCATGTTGTGGTGACCCCAACCATAGAGTT ATTTTCATTACTACTTTATAACTATAATTTTGCTACTGTTTGAATCATAATGTAAATATCTAAAATGCAT ATCTGATAAGTGACCCCCAAAGGAGGGGGAGGGAGAAGACACATAGGTTGAGAACGTGGGAACCTCTCAT GTGACCGCAGGCTGGCTGCCATATGTGTGACCGTGGTGATGGCCTAGGTCTCTAAGTCTGTGGACTTGGA CCTGTGTGTGTTTCTGCAGCAGTCAGTGAGAACATGAGGAGCAGGGGCAGAGTCATCGCGTAGATCACTG GCCCTCCAGTGCGGCACTTCCCGCTCTCCTCTGCTGCTGCATCTCAGTCCACTCCATCTTCTTCTCCTTC AGAAGCAGAATGTGACCATCATGGACCACCACACAGCCTCAGAGTCCTTCATGAAGCACATGCAGAATGA GTACCGGGCCCGTGGAGGCTGCCCGGCAGACTGGATTTGGCTGGTCCCTCCAGTGTCTGGGAGCATCACC CCTGTGTTCCACCAGGAGATGTTGAACTATGTCCTATCTCCATTCTACTACTACCAGGTCAGGGAGAAGC CTCCACCCACCTGCCCATCCCCGCCCCACCTCCCATCTTATAGCTCAGCTGTGGAGACTCTGTGGTGTGA GGACTCAGGGCTGTACATGCCAGCTGAGTGCCACCTTGTGCTCTCTAATCTAGTGTACCCAATAAAAAGA TACTAAAAGCAGGTGGGCCTCTACCTTCTGTGACCATGACAAAGGAAGCCAAGTGCCCACTGCTGTGTGG CTCATGTGGGGCCCAGGAAGCAGTACCCTGAGCTTTGGCTAACCCTTGGTGGAGTGGGCCTCTATGAGTG CAGTAGAGGGTCCTCTGCCTCTCCAGCATCCCCCGGCCCAAGCCCAGCCAGAACCCCACCCCCAACTCTC CTTCCTTTCTGCAGATCGAGCCCTGGAAGACCCACATCTGGCAGAATGAGAAGCTGAGGCCCAGGAGGAG AGAGATCCGATTTAGAGTCTTGGTGAAGTATGTGGAAGTGGGCATGTCCCTCTGTCTGTGCTGTGTCCCT TTTACTGAGGCATCCTTCCTGACTCCACCATGAAGGGCAGGGCATACGGAGAATGTCCCTTTTGGTGTTG GCTCCCAGAACTCTAGACACCAATTCTGAGACTGATTTGCTTCTGTAGTTCAGATTTGCCAAAGAGACTG AGTCAGGATGCACAGGGGCCACCAGTCAGAAAATGAGAAGTAGAGGTTCTGCCCCCCACCCCAGGGATAC CCCTGTTCCGTCTCTTCGGGACACTAACTCACCACTCAGCTGTCTGTGTATCTGTCTGTTCATCCCCACA GAGTGGTGTTCTTTGCTTCCATGCTAATGCGAAAGGTCATGGCTTCACGGGTCAGAGCCACAGTCCTCTT TGCTACTGAGACAGGGAAGTCTGAAGCACTAGCCAGGGACCTGGCCACCTTGTTCAGCTACGCCTTCAAC ACCAAGGTACCCACTTAGGTGGAAGGAGTCTGGAGTGAGTGTAAGCGGCCCCTTGGGAGCTGGGGGCTGA TGTTCAACGGTTGTCACACAGGTTGTCTGCATGGACCAGTATAAGGCAAGCACCTTGGAAGAGGAGCAAC TACTGCTGGTGGTGACAAGCACATTTGGGAATGGAGACTGTCCCAGCAATGGGCAGGTGGGTGGCCGGAG ACTCGGCAGAGAGGCTTGGGAGGACCAAGGAAAGGGTCCGGGGGACTCAGCATGACACTCTGGGTTCTTA TGCCACTTTCCCAATGGCACACAGTGTTTTCCTGTGAATTGACCAACTCTTACTTGTATGTGTTTAGATT ACTTGAGGGGATTCATTACATTTATTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG TGCAAGTCCCACAGCTTGCATGTAGAGGTTAGAGGACAACTTGAAGGAGACAATTCTTACCTTCTGTAAC ATGAGTCCCTGAGTTTTTCAGGCTTGGTGGCACTTTTACCTCCTGAGCCATCACTGCAGCCACAACCTAC TTAAAAAAATTTTTTTTCCAAAACTTCTTATTTAACTGGGCATTGTGATGTACACTCGCAATCCCAGCAC TAGGCAGTAGAGGCAGGAGGATCAGAAATTTGGGCGCAGCCTGGACTCCATAGGAAATCTTGACTCAAAT TCTCCCTCAACCAGGATTCCTCCTTAGGGGCTGAGAGATGGCTCAGCAGGTAAGATGGATTGTGGCACTT TCAAAGAACCACAGTCCAGTCCCAGCACCTATGTCAGGTGGCTCATAGTCTCCTATAACTTCAGCTCTAG GGACTCCAACATTGTCTTCTGGCCTTCCTAGGCACTCACACTCTGTACATAGACACAATCTCTGACACAA ATCTTTTTTGGGGGGGGGAGGGGTAAGGTTTGTTTGTTTGTTTATTTATTTAATGTAAGTGAGTACACTG TACAGATGGTTGTGAGCCTTCACGTGGTTGTTGGGAATTGAATTTTTAGGACCTATGCTCGCTCCAGTCA GCCCCCCTCACTCTGGCTCAAAGATTTATGTATTATTATATCTAAGTACACTGTAGCTGTCTTCAGACAC ACCAGAAGAGGGCGTCAGATCTCATTACGGGTGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTC AAGATCTGAGGAAGAACCCTTATTAAATTGCTCTTACCTTCTGAGCCACCTCACCAGCCCGACATAAATC CTTTAAAAAGAATTTTTTTTTAAATGTATTGTGGCTAGAGAGATGGCTTAGCTATTAAGAGCACTGACTG CTATTCCAGAGGTCCCGAGTTCAATTCCCAGCAACCACATGATGGCTCACAACTCACAATGTAATAAGAT CTGTTGCCCTCTCCTGGTGTGTCTGAAGACAGCTACAGTGTACTCACATACATAAAATAAATCTTAACAA ATGAGCAAAGACCGTATCATTCTCACAAAGGAAAAATGGGCCGAATATATGAATTTTGTCTAGCTACCCT CATGCAGGCCTGCACAGCCTTGTGTTTTGAGCAGAGACAAGTATGAGCAAGATGTTAAGGCTTGGTTAGG ACTAATTTGAGTTAGTCTCTCCTGTGTTGGAATGATGCAAAGGCCATCGAAAAGAAAGTGCATGAGAAGA GATTACAAAAGGCTTAGCTTCCCACAGTGTGATGAGTCCCACACTGCCTGAGACAGTTTCCTGGAAAATC AAAGTTATTTTAGAGAATCTGTTGTTTCTAGCAACTTCCAAGCAAGAAGCAAGGTTCGTCTTCTTTTTGA GGAACCTTGTGTTTTCCAAAGCTAATTGGGGAGGCTAGGGACTTCTGGGTCTTCCATTGAAGGGAAGGGC CCACGGGAGCTGGTTCAGCTGCAGTGAGGGGCAATCTGGACTTGTCCATTGGCTTTCCCTTAGTATCTGG ACTCTTTGCCTGCTCAGTGTGGCCAGTGTTTACACAGACCATCATAGTAAAGGAAAGCAGTGTTTTTGTA GCTCCTGGCCTGAGATCTCCGCTTCCTTGTCTTTTGTAGACTCTGAAGAAATCTCTGTTCATGCTTAGAG AACTCAACCACACCTTCAGGTGAGGCATCTCCCATATCCCTGACTCCTGGGCCCCAGTCTGGGATCAGAC CAAGGAACAGGGTGTACACAGGATGTCTTCTCAGACATGTCCAGGGGCAGACAGAGTTCCGGTCTGAGGT TTGTCTTCTGAGTGGGTTGAGGAGCAAGATGTGGAAACTGAGTCCCTCTTATAGATTTGCAGGCCTAGAG CCCACTGGTCACATGACCAAGGAAGGCCAGACTACCGTGTGACTCAGTGGGCATGGGTCTGTCCTTGCAA ACCATGGGCATTGATGCTGGGCATCCTGAGTCTCCATGTGTGTCCTTCTTGGCAGGTATGCTGTGTTTGG CCTTGGCTCCAGCATGTACCCTCAGTTCTGCGCCTTTGCTCATGACATCGACCAGAAGCTGTCCCACCTG GGAGCCTCTCAGCTTGCCCCAACAGGAGAAGGGGACGAACTCAGTGGGCAGGAGGATGCCTTCCGCAGCT GGGCTGTACAAACCTTCCGGGTGAGACAGGCTTTAATGAGTCTATGCCAAACACATCTGGGCCTGGTGTC CTGGGTGTCATGGCCTCTGCTAGGTGTAGGGCGGGGGTATGGGCCTGGCATCTCAGGTCAAAGCTGCTTC CCCAACCCTCACCCCACCAAGAAAAATGTCACTCTGGTGTTCTGCTTCAGGTGGGAGGTGATAGCTGTCC AGCTCCGGCCCTGACCTTATGTCTCCAGGGCTCTGAAAGCTTGGCCACCTCGTAGTAGTGAAGTCTTCCT TCAGTCTTCCAGCTCTGCAGAGGAAGGGACACAGGACACACTGCTGAGCTCTCGTGACCTGACTTTATCT TCTCAAATCTTTACTCTCTGGGTTCCTCCCTGCTGGTAAAGGAAGCTTAACACTGAAATGAATGGACAGC CATGGGAATCCCAGCAGACTTGATTCCAAGACTCAGGCCTCCTCTATGAGCTTTTCTATCAGGATAGGGA AACCCCAGACATCCCTTGCTATGTGGGAAGGGATAAATATAAGATGACAGCTTTACTATCCAGGAAAATA TCACTAACCACTGTGTGCCAAGCCTAGGATTCTCTGAGCGCCTCCAGCCCCTTTCAGATGGCACGTTGTG ATTCTGTGATGCCGCCATTCGGTATTTGACCCATGGTGAGGGGTGGTGACGGTGACGATGAATGCCATTC TGCCTAACTTTCCAGGCAGCCTGTGAGACCTTTGATGTCCGAAGCAAACATCACATTCAGATCCCGAAAC GCTTCACTTCCAATGCAACATGGGAGCCACAGCAATATAGGCTCATCCAGAGCCCGGAGCCTTTAGACCT CAACAGAGGTAAGAGCCCCTCTGCACCCTACTGGGCCTTCCCTGCTTGAGCTGAGGCTATCTGAGCCCTG AACCAGTAGTCAAAGAATCCACCATGCCCTTCTAATGAAGGCAGTCACCTGGAACTGCCAGGCAGCTGTC TCCCTGGCCACAGGGTCTGTTGGTACAGAGCTTCAGGCTCTGAGGGAGAGCGGGTCAGGAGCCCTGAGAG AGTGGTAGTGGGCACAGGGAGCTGGCATCAGCAGGTGGGTGGTGGCGATAGCTGTGCCAGGCACTGTGCC GGCCTCTTGGCCCACATGAACTGCCCACCAAGGGCTAAGGTAGCATGCAGGAATGCTCAGCTCAGCGTCT GGGGCTTTCCGCAGCACAGCACAGGAGATCTGTACCATTTCTGTCTGTCTTTCCAGCCCTCAGCAGCATC CATGCAAAGAACGTGTTTACCATGAGGCTGAAATCCCAGCAGAATCTGCAGAGTGAAAAGTCCAGGTAAG GATGGGCAGGGATGCTTAGCTGGTACCCAAGATTGCCCGCGAGATGCTTCTTGTGACAGAAGTGTGGCCA AGTACTCCATGCTCCCTGCACTGCTAGGGAAGGCTATAGCCAAACTCCTCTGGGAAACTCCAGAGTGCTG AGGGTCCCCAGCTGAGGGGATGGCTTGTCACAAGCTGGCTGGTGACCACACTTGGTGGTGGGAGAACACT TATCCTGGGGTAACTGGCAGTCACCCATGGCTCCCTTTTCTTCTGTAGCCGCACCACCCTCCTCGTTCAG CTCACCTTCGAGGGCAGCCGAGGGCCCAGCTACCTGCCTGGGGAACACCTGGGGATCTTCCCAGGCAACC AGACCGCCCTGGTGCAGGGAATCTTGGAGCGAGTTGTGGATTGTCCTACACCACACCAAACTGTGTGCCT GGAGGTTCTGGATGAGAGCGGTGAGCCCCCGGCCCTGGACAAATCTCTACTTCCCTCAGTCCCATCCCTG AGAACTGGGCCTGTGTCTGTCCTGATCTACTTCCATCCCAACTCATGGGAGAAGCTTTCTGGCCTTCACT GGTAGTGAGGCCTCCCCCTAGCCTTGGACGAGGCACTGGAGGAGCAAAACCTGGCAGTGTCCATCATCAT CCAGGAGAAAGGGAGTCAGCCTGAAACTTAGAGACAAGAGACTTGCATCCTTGGGGCTGTGAGAATACAG ATGTGGTCCAGATGAGATGCATTAATAATAAAGTCCTTGTCTCTAGAAGGAAGTGAGGGGAGTGGAGAGG AGTTAAAAGCAATGTGCTAACACGCACTAAACGTGCCCAGGGAACGGGCACTGTGCCAGCGATTTCCCAA ATGTAGTTTTTACTCATTGCCAGCACAGACTATGTCATCACATCTGTTTCTCAAGAAGCAGCTGGAGGTC ACAGGATTTGGACACTCGGCTGAGAAGTGGGAACCAGAGTTTGCGCCTGGGTCATGCCGTGACTCTCCGA ATGCTGCCTCTTGGGACTCCTGAAGCCCAGCCACCACAATTTACATTGATCTGTTAATATCTCAGGAGGT TGTAGCCATAAACCTCTCTCTAAAAAAGCAACCAGCCTCTGGGGCGTATATCAATCTCCAGTAGACTTAG TCACTAAGTTTGAGGTTCACCTGGGGATGTCAGAGACTCAAGTATGGGCCACAGAAGGATTGCTTAGTCT GTCAGGACAAAGCCAATATAGGTGCCCACGTGGGGGCAAATTTTCAGCCTACCCTGGCAGCCCTGTCTTG GCACCAGCTCCTGATCTTTGCTCTTTCTGGAGCAAAGATGTCAGAGTCTTCCCTCCCTTGCAGATGGAGA ATAGCCATCATCTGAGAGACCCAAGTAGGGCTTTCCCCACCCTGACACCTTTCTTTCTTCCATTCAGGCA GCTACTGGGTCAAAGACAAGAGGCTGCCCCCCTGCTCACTCAGCCAAGCCCTCACCTACTTCCTGGACAT TACGACCCCTCCCACCCAGCTGCAGCTCCACAAGCTGGCTCGCTTTGCCACGGACGAGACGGATAGGCAG AGATTGGAGGCCTTGTGTCAGGTGAGGCAGGGGCAATTCAGTGGAAGGAATAGAGCCCAGAGCAGAGTTC AACCTCTGAGACCATCCTCCTGAGTCCCTAGGTGTGCCTTGTCCGTGTCTGGTCGGGGCTAGGCGTCGTC TTCTGCCCCGTCTCTAGTACACAGCAGTATTGAGTGAATGAGTTTCTCTGGGAATCAGTATGCTGTAACC TGTATTCATCACATGTCCCATTTCTGTGACTAACTGCATGTCAGAAGGAACTTAAGGGAAGGAGGGTTCA TTTTGGTTCATGAGGGTCCAGTCCATCATGGTAGGGTGTCATGACAACAGGAGCATGAGTGACAGATCAC ATTGCTTCTGGAGTGAGAAAGCAGGAAAGGTGGACACCAGTGTCCTGCTCAAAGTCCCAGACCATAGGAA TTCGGGGTGGGTCTTCCCAGTCAAACCTCTAGAGAAACACCTTCACCAAGGTGTGTTTCCACAGTGATCT GAAATCCAGTCAGGTTCACTGTGAAGAGTAACAGCCAAAGTGCACTTCCTTAGAACCTGACTAAAGGGGG AAGCTCTATGGATGGCCTTTGTCTTCCCAAGTGTGGACCACGCTTGGTGGTGGGTAAATGGGGGTACTGG CGGGTGGGGAGCTGAGTTAATGAGTTAGTGGGGTGATGCATTAAGTCACTTGCGACTTTAGAGTCCATGT CTTTATAAGAGTCACTGGACAGCAAGCCATGGGACACATTGTCATTGTCTCAACAATCCTATATTAATGG CTGCATCCACCTAACTCCACAAGGATTAGGGTTAAGCTAAGGAGGGACATCCAGCCATGACTTGAACTCA GATCCAGTTCGTTCCAAGTTACAGTATAATAGAGAAATTAGCCCAGCTGGGTTGGGTGGTGCATTCTTGT GATCCCAGCACACAGGAGGCTGATGCAGGAGGATAGTGAGTTAGAGGCAAGTCTGGGTCAACAATGAGTT CAAGACCAACCTGGGCTATGTAGCAAGACCATGTCTCTACATCCTCCCCAAAGAAAGAGGACACTGAGCC CTGCCCATGAGTTGCTGATGTTGGGAGGCTTCTGTGTTTTGATTTTTGTCTTTGTTTTGTGGGTACTAGT GTTTTCCTTCAGACCTGCACACATGCCAGGCTCTACCACTGAGACAAACTCTCTCTTCTCTGACTTTTAA TTCTGAGGCAGGGTCCCACTAAGTTATCCAAGCTAGCTCTCAATTCGGTCTCTAACCCCTGCTGGTCTTA AACTTGGAATTCTCCTGCGTCAGCCTTCTAACTAGTTGGGGTTGCAATCCTGCCCCACTGTCATCTTAAT TCTATTCATAGTAGAGTTCTAGGCACAATTGAGAAATGCTACTAGCTGCCCCAGGTCCTTGCTTCCCGTC CACCGGAGTAGCCTCAGCACCCTGGATAGCGCCCGTCTTTTGCTGCGTTTGTTGAAGGAATGAATGGATA CTGGGTGGGGCAGCTCCTACACGCACGCAGCTCCTACACGCACGCGCGCACGCACGGCAGGTCCTCCGAT CCCTCACACTGCCCTTGCCTTTCAGCCCTCAGAGTACAATGACTGGAAGTTCAGCAACAACCCCACGTTC CTGGAGGTGCTTGAAGAGTTCCCTTCCTTGCATGTGCCCGCTGCCTTCCTGCTGTCGCAGCTCCCTATCT TGAAGCCCCGCTACTACTCCATCAGCTCCTCCCAGGACCACACCCCCTCGGAGGTTCACCTCACTGTGGC CGTGGTCACCTACCGCACCCGAGGTGAGACAGGCAGGCTCTGGTGTCATCTGCACCTGTCCTTCTAGGGA CAGCTGTCCTCGATTGCTTTCTGTTGCTGTGACCAACACTGACTGCCACCACTTTAGGGCAAGATCCAAT TTAGGGGTTTAACTTAAAGTTTACAACTGTCACCAAAGGAAGCCAAGGCAGGAACCACAGAGGAACGTTG CTTATTGGCTCCCTCTGGCTTGCTCAGCTGCCTTCCTTACCAGCTCAGCACCACCTGCCCAGGATCAGCA CTGCTCACAGTGGGCGATGCTCTTCTCCATCATCAATTAGCAGTTAAGAACGCACCCCACAGACATGCCG ATAGGCCTATCTGATGACAAGCCCTCAATTGAGGGTCCCTCTTCCCAGGTGTATCAAGTTGATGGCCGAC ACTGGTCACCACAACAGCTGTTCAATACCTTGGGGCTTTGTCAGCTACATGCTGCAGTGACAGCCACCTG CCAGAGTTGTCAGTGATATAAGATGCTGTGTAGGGCACAGTGTCACAAAGCAGGAGGGAAGCTCGCTCCT TTGTTTACCATTCAGACCTGTAGGAGATGGCTTCCATGTGTAATTTTACGAGGAAGGAAAAGAGTCATGG AGAGGTTAACCCACCTGTTCAGAGACACCTGTCATTAACTAGAGACACAAATTCAGGGAGTTTACCTGCT GAAGCTAAGAACTGGACCATTGTTACAGTCTCTGGCTGTTGAATAGGCTCAGACGTGAAAAGCCTTGAAT GCGTAGGGAACTCAGTTACTTTTGGTCATCCTAATGATGCAGGTGGATGCAGGGAACATTTTCTCAATTT TTTTCATTCCCTCAGTGTTCCCATCCTGTGATGGCCGTGTCTCACCTCCATGCCAGGGGCCACACTGGGT GTGGAAACTGCAGACAGGCCCTGCCCTCACAGAGCAGCCAGAAAGTCAAAAAAGAGTTCAGGCATGGACA TACTTGGACATATGTTTGTGTGGGTGCTCAGAGGCCCCCGGTATCACGAGGGAGGGAGTGGGGTGTGTGT GAAGCTGCCCTAGACTAGGAGGTCAAGTGTTCTAGGCTTGGTTCCTCCACTGGATGGACCTCCCTGTTCT AACCACTGTAACTGAGCTCTGTTTGAGCTGGAAGGGCTGGAAAGCGTCCACATCTCCGAGATCTCTCTCC TTGGCCAAGAGCGCAGTTTGTAAATACTGCACAGAGCCTGTGTCTGTCCATCCTCACTGACCATACCACA CCATTGAATGAGAGAGGAGATGCGGGTGAGCCCTGACAAGGCTGGTTCATGCAGACCAGAACCAAACAAC TATTCCAGTTGCTAAAGGCCTCCCTTATGTGTCAGGCCATGGGGAAGTGAATGTAACCCAGAGGACACTG GGGTACCCACAAGCCCAGGAAAAGGCTTCTGGGGAAGATGGAGATGCCAGAATAGACAGGGGGCGTGGAA GACCACCCCTCACCCAGAGTTCCTATTGGCAAAGGCCTCCTTCATGAGGACACTCAGCAATGGCCAGCCC TGGGGATCATTTCACACTGCGTGGGCAGAGCCTTAGTGGGGAAAGGATGTTGGGAGGGTCCCTAGACAAG CACTTGGGAGATGCTCTAGCGTAAGACTGAGGAACCTGAGCAGGAAGTTGAGTCCACCATGACTTTAGTC CCTTGCTCCGTTGCCTTCTGTGTGACTCAGTTTCTACAATGGCAAAGGCGCAGTTGCTCACCAGGCCCCT GACTGCCTTCCCTGATCTGTATAGACAGCTGGGAGAAGAGGTAACTCCTCATTCAGGAAGCAGCCACCCA ACGGGGCTTGACACTGCAGGAAGAACACCCAGCCGGGTTGCACGAAGCCCACGGGGCTTGTTAGTCAGGC CTGTGTTTTCCTTGCAGATGGTCAGGGTCCCCTGCACCATGGTGTCTGCAGCACTTGGATCAGGAACCTG AAGCCCCAGGACCCAGTGCCCTGCTTTGTGCGAAGGTAAGCACCCACTCGGGGCCCTTTGGAGCCCCTAG CCCCTGGGGATTCTGCAATGCAAGGGAAGGGTCTAAGACTGAGTCTCCTCTCGGCCCATAAAGGTTTAGG GGGTAATTCTTTGTCTCCTCGTTTTGCCATCTGGTTACAAGGTGTCGTGGAGTCCATGACACAGATACAG TGATGCCTGTGGTGGGATCCAATGAGACTGGCCCAGTGTGGAGAGAGGCCGTTGCCTGGGCTGGGAAGAT GAAGATGTGCACAGGTCCCCGCCAGGCGCTTAGGTCCCCAGCCCTATCCTGCATGTGCTCCTAGCCTGGG ATATCTGCAGACCAGGGTCTTTCTCCTTCTGTCTTCCTGCAGTGTCAGTGGCTTCCAGCTCCCTGAGGAC CCCTCCCAGCCTTGCATCCTCATTGGGCCTGGTACGGGCATTGCTCCCTTCCGAAGTTTCTGGCAGCAGC GGCTCCATGACTCCCAGCACAAAGGTACAGCTCTTACTCAATAGGGAGTGTGCCTGTCACTGTGACCGAC CTGATCATTACACAGGCACATCAAATTACTACACCATATCCCATAATCAGGTACACAGATGTGGAGTGTT TGTGCTCTGCCCACTCCACGTCTCCATGTTTCCTTGTTAATTTTTAAGGTATCTAGAGGAGTGTGTATGT CCTGGTACGCATGTGGAGGTCAGAGGTCAACTTGTTTTACTTCTTCCAGGAATCAGATTCAGCTTGTCAG TCACGACGGCAAGCACCTCTCCCTGCTGAGCCATCTCATCAGCCCATTTTGCTGGGTTTTAAATGCTTGC CTCCTTGCTGGACCATCCATTAATAACACAGAGATTAATAAGCCTGTGTAGGGGTCCCCAGAGTGAAGGG GGAATGAGAGTAACAGACTTTAGATGAAGACATTTTCTTTTCATTGGAGAAATAGCTGGGAAGGATGGAC AGCTCTGTCTGGAATCCTCCAGATGAGGCTTTTCTGGAAGAAACCCTTAGCAATGTTTGACCTTCAAGAT GCCTGAGGCCTCTGCACCTATATTTGATGTAGCATCTGGGTCAGGGGAGATGATTTTCACAAAAGAAGGC CACGAGTTTCTGATATTCCACTAAAAGAATGTCCTTAGCATGTTACACACACACACACCTATACATATTT GAGACAAAGTCTTGCTATTGTAGCCCTGGATGAACTGAAAATTCCCTATACAGAGGAGGCTGTCCTCAAA TTTGCAGGAAATCAATCCTCCTGCTTCTAATTCCCAAGAACTGGCTATAATAATATTTTAAAAGAAGAAG TAAGATTAAAATAGAATATGTCAAGTAAAAGAAATAGCAATGTGTAGACATAATCAGAACTGATACTTTT TTTTCCTGAACCATGTTTTTTAAGAGTGCCACCATTAGCTATAAAATGGGAATAATGCCAGATCAGAAAC CTACCCATTGGGTACCTGGTGCTCACAGGTTGCCCTCTGGTGGCCGTTGTGGTAACTGCAACCTTCTGGA TCTTCAAGGGCTCAAAGGAGGCCGCATGAGCTTGGTGTTTGGGTGCCGGCACCCGGAGGAGGACCACCTC TATCAGGAAGAAATGCAGGAGATGGTCCGCAAGAGAGTGCTGTTCCAGGTGCACACAGGCTACTCCCGGC TGCCCGGCAAACCCAAGGTAAGTGCAGCCACCCAGCAAGCTGGCAGAGCACGCATGATGGCCCCTGCCCA GGCTATACAGCCCTTCCACTCCAAGCCTGCAGTGAAGACAGTTGCACAGTGGCAGGGAGAGATGAAGAGC ACGATGACAACATCAAGCTCACTGTAGTTTAATCTCCACAGTGCAAACACCTGCAGGTGCCCAGCATCTC TGGGACGCAGGGCTGGGCCGGGCTGGCTTTACACTTAGTTCAGTAAGACTGGAAGGTGGAGCTGATTCCC CCCAGGGCCCTCAGGTTGGCACTCTGCTCCAGGGGCATGAACAGTGCCTACACACTTTCCATTCAGGACC ATGTGAACATTCCCTGAAGACCCCATTTGCATCCTAAAGCTGGACCACTGGTCATCTTAGATGAAAAACA AAAACAAACAGACTCCTGACTGACTGCAGGACCTTCTTTATAAATGGAAAGCCTAGGAGCCTATACCTGA GCTACAAATCAGAGCTGGGGCTAATACATACTGGGAAGCCCCCTCTTTCCGGGGTGTAGACAGGCAGATC TCACAGCCCTGGGCAGAGCTATAAACTTGATGTCTCTAAAGTATGGTGACCTGAGGGAAGCTTTACGGGT TCCATTGGCCAGGAGGACAGGATACTTGTCTATCCTGAGCTGAGGTCCTGACTGAAGCCCTCAATGGCCC TCTTCCCCTCCCCATGGCCAGTGTGCCTGACCACAGTGACACCAGCATCCAGGGCTGGTCCCACCGTGTC CTCTGCTTTTAGGTCTACGTTCAGGACATCCTGCAAAAGCAGCTGGCCAATGAGGTACTCAGCGTGCTCC ACGGGGAGCAGGGCCACCTCTACATTTGCGGAGATGTGCGCATGGCTCGGGATGTGGCTACCACATTGAA GAAGCTGGTGGCCACCAAGCTGAACTTGAGCGAGGAGCAGGTGGAAGACTATTTCTTCCAGCTCAAGGTA TGGGAGCTGGGGTGGGACCCTCCAGAGGGGAGTAGACCAAGGTCACAAGCCTTTGGAACTAGGACCAGGG ATGCAGAACTGGGGTGGGGGAGGTGTGTGCCCTGAGATCCTCGGCACCACTGCAGAAACCTGGGGCCGGC TGGGCTCCTCAAGCCTGGTCTTTCCATGCCATAGTGGTTGCCAGCTAGCTGAGAAATCAGGCCAGTGTGT TCCATTTCTCTATATCTGGGATGAAAGCAGCAGTCTCTGCTCCCCACACTCCTGCCTAAATGCCCATTGA GCATAACAGGGCTGTGGACTGTCCCCTACTGCCTATGGGGGACAGACACTTATTTACAATAGGTTGCTGG CTAGGTGCCCTCATTTCTCACAGGAAGGACTCTTGGTAGCAGGGTCTGCCTTGTGCCAGGTGCTCACCAA TGATTGCCCCGTGGTGACCTTTCCTTCTGCCCTTCCAAGGGCTTTATGACGGAAGAATGACTGTTCCATC ATTTTGCAGATGGAAGGGGCCCTGGTGCCCTAACAGCGAATGTGAGAGCCCAGACACATGGCCCCTCTCT CCTTTTCCCTCTCTCTGTCTCTCTGGTTCCTAAACCTGCCTTTAAGGCTCGAAGTTATGCTCCCCATTTA TATTAAACATCTACTATGTGTTAGAGGTAAGAAGCAGTCAACAAAACAGGTGAAAATGTTGTCAATATCA CGATAGATCACAGAGCACACCTGTATCGCCAGCACTGGGGAGGTGGAGGCCCAGGGGTCAAGAGTTCAGA TTGTCCCTGGGTTTCATAGTCAGTCCAAGGCTGGACTGGGCTATATGAGATCCTATTTCGAAAGTACAAA AGCAAAAGACCTACCTAAACGTAGCTTACATTCTGGCAGAGTTGATCAAATCAAGCACATAGGGTGCGCA CTGCTTTGGGTAAGACGCAGAACAGCAGCTCAGAGCTGGGGTGTTTGTGGTTTAATGAGGTTTGGGGAAG GGGTCCCCGGGAGGTGACATTTGAGAAAAATACCTGAAGAAGGAAGAAGCTTTGTGCACACTGGAGACAG AGGTCCAGACTATTCTGTGGGGCAGCAACATGTTCAAAGGTCCTGGGGTGTGGACTATTCTGAAACCTGT GATGACCAGCAAGGGTATCATGGGTACTTCCCAAAATGTGTTCAGAGCTTGCCAGATGTGTTCATGGATG TTAGACATCATTAACAATGGGCTGTAAGGGCAGAGGCAGTGCACACCTGTTAGTCCTAAGGCTTTGGGGA AGCTGAGGCAGGAGAGTTGTAAGTTCAAGTCAGTATGGGCTACCTTAGGCTGCAAGAATGCAGAGGACAT ACCTGTAACATCAGCTCCAGGGGATCTGATCCCTCATATACAGGTACATGTATACACACACACACACACA CACACACACTTGAAAATAAAACTGTAGCCAGACATGGAGGCATGTGCACACCTTTAACTCTAGCACTCAG AGGCAGGAAATAAAATCCTTTCTTTTTTATTTATTTTAATTTAAAAAGAGAAAATTAGTTCCAGGGGTAT AGCTTAGGCCTTGCCTAGCCTGGTATATAAAAGGCCCCAGGTTTGATTTCTAGTACCAAGGGAAAAAAAG ATTCCAAGGCCAGACATTTGGTACACACGTGCGCACCCACAAAGAGCAACAGGTGTCTGCTGTAAGATTT CTCTCGGAGCCCTCAGCCTGCTGCTGATTTCAAGGCCTGAAGAATGGGAGGCACACACTGCCTGCCCTGC ACATCCTGATCTCAAAGCTCACATCCGATGTGGGATGAGCACTAGACAGGTAGTGGGACCGACATCCATC CATGAAGTCTGGGAAGAGGCTGAGGTGGGCCACACATGGGCAGAGGAGAAACCAAGCATGGGTGTAATGG CAGCAATCCAGACAAGGGGGTAGACACAGGGCTAGCGAGCGCCTGTGTCACCAGGAAGCTGTGGCAGCCC TAGGGTTTTGTCCAAGCCTAAACATGATAACTGAGAGGTGGCTGGGCCAGCAGGGTGACACTGTCACCTT TGTTTCCCCCTCACAGAGCCAGAAACGTTATCATGAAGATATCTTCGGTGCAGTCTTTTCCTATGGGGCA AAAAAGGGCAGCGCCTTGGAGGAGCCCAAAGCCACGAGGCTCTGACAGCCCAGAGTTCCAGCTTCTGGCA CTGAGTAAAGATAATGGTGAGGGGCTTGGGGAGACAGCGAAATGCAATCCCCCCCAAGCCCCTCATGTCA TTCCCCCCTCCTCCACCCTACCAAGTAGTATTGTACTATTGTGGACTACTAAATCTCTCTCCTCTCCTCC CTCCCCTCTCTCCCTTTCCTCCCTTCTTCTCCACTCCCCAGCTCCCTCCTTCTCCTTCTCCTCCTTTGCC TCTCACTCTTCCTTGGAGCTGAGAGCAGAGAAAAACTCAACCTCCTGACTGAAGCACTTTGGGTGACCAC CAGGAGGCACCATGCCGCCGCTCTAATACTTAGCTGCACTATGTACAGATATTTATACTTCATATT >gi|6754872|ref|NP_035057.1| nitric oxide synthase, inducible [Mus musculus] MACPWKFLFKVKSYQSDLKEEKDINNNVKKTPCAVLSPTIQDDPKSHQNGSPQLLTGTAQNVPESLDKLH VTSTRPQYVRIKNWGSGEILHDTLHHKATSDFTCKSKSCLGSIMNPKSLTRGPRDKPTPLEELLPHAIEF INQYYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDARN CSTAQEMFQHICRHILYATNNGNIRSAITVFPQRSDGKHDFRLWNSQLIRYAGYQMPDGTIRGDAATLEF TQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGLKWYALPAVANML LEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASLWKDRAVTEINVAVLHSF QKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITPVFHQEMLNYVLSPFYYYQIEPW KTHIWQNEKLRPRRREIRFRVLVKVVFFASMLMRKVMASRVRATVLFATETGKSEALARDLATLFSYAFN TKVVCMDQYKASTLEEEQLLLVVTSTFGNGDCPSNGQTLKKSLFMLRELNHTFRYAVFGLGSSMYPQFCA FAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRSWAVQTFRAACETFDVRSKHHIQIPKRFTSNATWEP QQYRLIQSPEPLDLNRALSSIHAKNVFTMRLKSQQNLQSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGIF PGNQTALVQGILERVVDCPTPHQTVCLEVLDESGSYWVKDKRLPPCSLSQALTYFLDITTPPTQLQLHKL ARFATDETDRQRLEALCQPSEYNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQDH TPSEVHLTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCILIGPGTGI APFRSFWQQRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPGKPKVYV QDILQKQLANEVLSVLHGEQGHLYICGDVRMARDVATTLKKLVATKLNLSEEQVEDYFFQLKSQKRYHED IFGAVFSYGAKKGSALEEPKATRL Vaximmutor panC Mycobacterium tuberculosis H37Rv VO_0002536 885459 15610738 Rv3602c BX842583 NP_218119 83332 4044280 4045209 - pantoate--beta-alanine ligase 5.96 29602.42 309 catalyzes the formation of (R)-pantothenate from pantoate and beta-alanine >gi|57116681:4044280-4045209 Mycobacterium tuberculosis H37Rv, complete genome ATCAGTTTCTCCAATGTGATTCGAGGATTGCCCGGTATCCGTCCGGGCGGTCGGTGCCGGCGAAAGTTCC GATTTCAATCGCAATGTTGTCCAGCAGCCTGGTGGTGCCAAGCCGGGCAGCAACCAGCAGCCGACCGGAA CCGTTGAGCGGCATCGGGCCAAGCCCGATATCGCGCAGCTCCAGGTAGTCGACCGCCACGCCGGGTGCAG CGTCGAGCACCGCACGGGCGGCATCCAGCGCGGCCTGCGCGCCAGCCGTTGCCGCATGCGCTGCGGCCGT TAGCGCCGCCGAGAGCGCGACGGCCGCCGCACGCTGGGCCGGGTCCAGGTAGCGGTTGCGCGACGACATC GCCAGCCCGTCGGCTTCGCGCACGGTCGGCACGCCGACCACCGCGACATCGAGGTTGAAGTCCGCGACCA GCTGCCGGATCAGCACCAGCTGCTGGTAGTCCTTCTCACCGAAGAACACCCGATCCGGGCGCACGATCTG CAGCAGCTTTAGCACGACCGTCAGCACGCCGGCGAAATGGGTTGGCCGCGGGCCGCCCTCGAGTTCGGCG GCCAACGGACCGGGTTGCACGGTGGTGCGCAGGCCGTCGGGATACATCGCCGCGGTAGTTGGCGTGAAAG CGATTTCCACGCCTTCGGCCCGCAGTTGCGCCAGGTCGTCGTCCGGGGTGCGGGGATAGGCGTCGAGATC TTCCCCGGCACCGAATTGCATCGGGTTGACGAAGATCGACACGACGACGACCGATCCGGGCACCCGCTTG GCCGCACGCACCAACGCGAGGTGGCCTTCGTGCAGCGCACCCATAGTAGGCACCAACATCACTCGCCGGC CGGTGAGTCGCAGTGCGCGACTGACATCGGCGACATCCCCCGGTGCCGAGTACACATTGAGTTCACCGGG ATGGAACGCAGGAATCGTCA >gi|15610738|ref|NP_218119.1| pantoate--beta-alanine ligase [Mycobacterium tuberculosis H37Rv] MTIPAFHPGELNVYSAPGDVADVSRALRLTGRRVMLVPTMGALHEGHLALVRAAKRVPGSVVVVSIFVNP MQFGAGEDLDAYPRTPDDDLAQLRAEGVEIAFTPTTAAMYPDGLRTTVQPGPLAAELEGGPRPTHFAGVL TVVLKLLQIVRPDRVFFGEKDYQQLVLIRQLVADFNLDVAVVGVPTVREADGLAMSSRNRYLDPAQRAAA VALSAALTAAAHAATAGAQAALDAARAVLDAAPGVAVDYLELRDIGLGPMPLNGSGRLLVAARLGTTRLL DNIAIEIGTFAGTDRPDGYRAILESHWRN Virmugen A panCD mutant is attenuated in mice and induces significant protection from challenge with wild type M. tuberculosis [Ref1674:Sambandamurthy et al., 2002]. panD Mycobacterium tuberculosis H37Rv VO_0002538 885596 15610737 Rv3601c BX842583 NP_218118 83332 4043861 4044280 - aspartate alpha-decarboxylase 4.69 13595.37 139 Converts L-aspartate to beta-alanine and provides the major route of beta-alanine production in bacteria. Beta-alanine is essential for the biosynthesis of pantothenate (vitamin B5) >gi|57116681:4043861-4044280 Mycobacterium tuberculosis H37Rv, complete genome GCTATCCCACACCGAGCCGGGGGTCTAGCAGCTCGCCCGCGTTTTCGGGCACAAATGCCGGATCGTGGCC CATGTCGATCGGTTTGTTGTAAGCGTCGACAAACACGATCCGCGGCTGGTATGTGCGGGCCCGGGCGTCG TCCATCGTCGCGTACGCAATCAGAATCACCAGATCCCCCGGATGCACCAAGTGCGCGGCGGCACCGTTGA TGCCAATCACACCACTGCCGCGTTCGCCGGTGATCGCGTAGGTGACCAGTCGAGCACCGTTGTCGATATC GACGATGGTTACCTGTTCGCCTTCCAGCAGGTCGGCGGCGTCCATCAAGTCGGCATCGATGGTCACCGAG CCGACGTAGTGCAGGTCGGCGCAGGTCACCGTGGCGCGGTGGATCTTCGACTTCAGCATCGTCCGTAACA >gi|15610737|ref|NP_218118.1| aspartate alpha-decarboxylase [Mycobacterium tuberculosis H37Rv] MLRTMLKSKIHRATVTCADLHYVGSVTIDADLMDAADLLEGEQVTIVDIDNGARLVTYAITGERGSGVIG INGAAAHLVHPGDLVILIAYATMDDARARTYQPRIVFVDAYNKPIDMGHDPAFVPENAGELLDPRLGVG Virmugen A panCD mutant is attenuated in mice and induces significant protection from challenge with wild type M. tuberculosis [Ref1674:Sambandamurthy et al., 2002]. PCDHB1 Homo sapiens 29930 14195607 AC010223 NP_037472 9606 5 19530 22063 + protocadherin beta 1 5.03 82950.69 818 Also known as PCDH-BETA1; MGC138301; MGC138303 >gi|28558729:19530-22063 Homo sapiens protocadherin beta cluster (PCDHB@) on chromosome 5 CAGTAACCTGTTGCAGAAAAGTGAAAGTATATCCGCAACAGTTGGCTCTGATTGCAGAGAGCGCGCTTGT GAGAACTGATGGCGGGTACGCGCAGAAAATCTTTGCAAAACAGGCAAGTGGGATCTCTTCTCATTTTTCT GTGCATATCTGTGGGGGATGCGACAACTATCCGCTATTCAGTGGCAGAGGAAATGGAGAGCGGCTCGTTT GTGGCCAACGTAGCTAAGGACCTAGGACTGGAGGTAGGGAAGCTGGCTGCGCGCGGGGCGCGGCTGGTTT CCGAGGGCAACAAAATGCATTTCCGGCTCCACCGCAAGACGGGAGATTTGTTTGTGAAGGAGAAACTGGA TCGGGAGTCACTTTGTGGCAAAGCCGACCCTTGTGTTCTGCACTTTGAAGTAGTCCTGGTGGAGCCGCTG CAGTCCTTCCGGGCCGAGGTCAGGGTATTTGATATCAATGACAATGCCCCAGTTTTCCTAAACAAGGAGC CGCTTTTAAAGATTCCGGAGAGCACCCCTTTGGGTTCACGTTTTCCTCTGCAGAGCGCCCAGGATCTGGA CGTGGGCCTTAACGGTCTCCAGAACTACACCCTGAGTGCCAATGGGTATTTCCACCTGCACACCCGCTTC TGCAGCCACGGGCCTAAATATGCTGAGCTGGTGCTGAACAAACCCCTGGACCGAGAGGAGCAGCCTGAAG TCAACTTGACAATTACGGCGGTGGACGGCGGGTCCCCGCCTAAGTCTGGCACAGCTCACATCCACGTGGT GGTTCTGGATGTCAACGACCACGTGCCCCAGTTCTCGCGACTGGTGTACAGAGCCCAGGTATCAGAGAAC AGCCCCAATGGCTCTTTGGTGGCCACGGTGACTGCCGTGGACCTAGACGAGGGCACCAACAAAGCGATAA CTTACTCTTTAGCTCAAAACCCAGAAGCAATTCTCAAGACGTTTCAGATTGACCCTCAAAATGGAGAAGT TCGACTAAGAGGACCCCTCGATTTTGAAGCCATTGAAACATACGACATTGACATTCAAGCTACAGATGGT GGAGGCCTCTCTGCCCACAGCAAAGTCCTGGTAGAAGTGGTGGATGTGAATGACAATCCTCCCGAAGTGA TGGTCTCCTCTGTGTCCAGCCCACTCCCTGAAGACTCACCACCACAGACAGTAGTAGCCCTTTTCACTAT CAGAGACCGGGACATTCGAGTGGGAGGAAAAGTCACCTGCTTCCTCAGAGAAGACCTTCCCTTTGTAATC AAACCTACATTTGGGAATTCTTACTCACTGGTCACTGACAGAAGCTTGGATCGGGAGGAGGTCTCAGGCT ATAATATCACCATTGTTGCCATGGATACTGGACCACCTAGCTTGTCTGCCGAGACTATGATAGAGGTGCT AATATCCGACGTTAATGACAATCCTCCAATATTTCGGGAAGATTCCTATATCTTGACTGTTCGAGAAAAC AACAGTCCTGCGGTTTTTATTGGCAAAGTCCATGCTGAGGATCTTGATTTGGGTGAGAATGCCCAAATAA CATATTCTCTGTTGCCTCCAAAAAACGGAGATCTTTCAGTCTTTGCTTACATATCCATAAATTCAGGCAA TGGGAAGCTCTACGCGCTGAGAACCATGGATTATGAGGCCATTCAAGATTTTCAATTTGTGGTAAAGGCA ACTGATGGGGGCTTCCTGTCACTGAGTAGCCAAGTTACTGTCAGAGTGGTTGTCCTAGATGACAATGACA ATCGTCCAATGATCTTATACCCACTGCAGAACGGCACCTTGCCCTGCAATGACCTGGTGCCCAGGTCTGC AGAGGCAGGCTACCTAGTGACCAAAGTGGTGGCTGTGGATGGTGACTCAGGTCAGAATTCTTGGCTTTCA TATCATCTACTTAAGGCCACTGACCTTGGGTTATTTTCTGTTCAAAGACAAAATGGAGAAATCCATACAT TAAGGCAGATATCTGAGAGAGACCCCATGATGCAGAAATTGATCATTCTTGTTCAGGATCACGGCCAACC AGCTCTTTCCACTACTGTCTCACTCAACATCCTGCTGGTAGATGGCTTTTCAGAGCCCTACCTGCAGTTC CAGGATCCAACCAAGCATTCTAGAAAGGTAAATCCATCCACTAAATATTTGGTCATTTCTCTGGTCATCC TTTCCTTTCTCTTTCTCCTCTCTGTCATAGTGATCTTCATTATACATGTCTACCAAAAGATTAAATATAG AGAAAAGTTCACAATTCAAGAGCATTTCTATGATGACTGTAATTTCTCTAACAACCTGGTACAAGGACAA GGCAATGGATCCTTATCTCGGCCTTGTCCATATGAAATGTGTTCAGCCACTGGCACTGGTAATAGTGAGT TTCGCTTTCTTAAGCGTTTTATGCCCAACTTCCCTTTCCCTCATGCCACTGGGGAGATAAAAATGGAGGC TGGCTCCAGTTTGCCCCCAAATTCTGATAGGAATAAGTCTCAGAGATTAGAGGGCCATGACCAGGTATCT GATGACTATATGTA >gi|14195607|ref|NP_037472.2| protocadherin beta-1 precursor [Homo sapiens] MAGTRRKSLQNRQVGSLLIFLCISVGDATTIRYSVAEEMESGSFVANVAKDLGLEVGKLAARGARLVSEG NKMHFRLHRKTGDLFVKEKLDRESLCGKADPCVLHFEVVLVEPLQSFRAEVRVFDINDNAPVFLNKEPLL KIPESTPLGSRFPLQSAQDLDVGLNGLQNYTLSANGYFHLHTRFCSHGPKYAELVLNKPLDREEQPEVNL TITAVDGGSPPKSGTAHIHVVVLDVNDHVPQFSRLVYRAQVSENSPNGSLVATVTAVDLDEGTNKAITYS LAQNPEAILKTFQIDPQNGEVRLRGPLDFEAIETYDIDIQATDGGGLSAHSKVLVEVVDVNDNPPEVMVS SVSSPLPEDSPPQTVVALFTIRDRDIRVGGKVTCFLREDLPFVIKPTFGNSYSLVTDRSLDREEVSGYNI TIVAMDTGPPSLSAETMIEVLISDVNDNPPIFREDSYILTVRENNSPAVFIGKVHAEDLDLGENAQITYS LLPPKNGDLSVFAYISINSGNGKLYALRTMDYEAIQDFQFVVKATDGGFLSLSSQVTVRVVVLDDNDNRP MILYPLQNGTLPCNDLVPRSAEAGYLVTKVVAVDGDSGQNSWLSYHLLKATDLGLFSVQRQNGEIHTLRQ ISERDPMMQKLIILVQDHGQPALSTTVSLNILLVDGFSEPYLQFQDPTKHSRKVNPSTKYLVISLVILSF LFLLSVIVIFIIHVYQKIKYREKFTIQEHFYDDCNFSNNLVQGQGNGSLSRPCPYEMCSATGTGNSEFRF LKRFMPNFPFPHATGEIKMEAGSSLPPNSDRNKSQRLEGHDQVSDDYM Vaximmutor PE20 Mycobacterium tuberculosis H37Rv VO_0012371 885537 57116919 Rv1806 JLDD01000021 YP_177843 83332 2048071 2048370 + PE family protein PE20 4.19 8927.3 99 Rv1806, (MTV049.28), len: 99 aa. Member of the Mycobacterium tuberculosis PE family of gly-, ala-rich proteins (see citation below), most similar to Rv1788|MTV049.10|AL022021 (99 aa), FASTA scores: opt: 334, E(): 4.7 e-15, (59.8% identity in 97 aa overlap). TBparse score is 0.932. >NC_000962.3:2048071-2048370 Mycobacterium tuberculosis H37Rv, complete genome CATGGCGTTTGTTCTTGTCTGTCCAGATGCGCTGGCCATCGCGGCCGGTCAGTTGCGCCATGTTGGATCG GTGATAGCCGCGCGGAATGCGGTCGCGGCACCGGCAACTGCCGAATTGGCCCCGGCGGCCGCTGACGAAG TATCAGCTTTGACTGCAACACAATTCAACTTCCATGCCGCCATGTACCAAGCGGTCGGCGCCCAGGCGAT CGCCATGAATGAGGCGTTCGTCGCGATGTTGGGCGCCAGCGCGGATTCTTACGCGGCTACCGAAGCCGCC AACATCATTGCTGTGAGCTA >YP_177843.1 PE family protein PE20 [Mycobacterium tuberculosis H37Rv] MAFVLVCPDALAIAAGQLRHVGSVIAARNAVAAPATAELAPAAADEVSALTATQFNFHAAMYQAVGAQAI AMNEAFVAMLGASADSYAATEAANIIAVS Protective antigen A selection of previously identified protective Mycobacterium tuberculosis DNA vaccines were re-formulated as proteins and administered with a Th1-inducing adjuvant to help stimulate the relevant immune responses necessary for protection. One candidate (Rv1806-1807) induced protection in the guinea pig aerosol infection model 30 days post-challenge on the basis of reducing the bacterial burden of M. tuberculosis in the lungs [Ref329:Vipond et al., 2006]. PepA Mycobacterium tuberculosis H37Rv VO_0010953 886924 15607267 Rv0125 CP003248 NP_214639 83332 151147 152214 + serine protease PepA 4.91 30958.56 355 Rv0125, (MTCI418B.07, MTB32A), len: 355 aa. Probable pepA (alternate gene name: mtb32a), serine protease (EC 3.4.21.-) (see Skeiky et al., 1999), highly similar to other proteases e.g. HHOB_ECOLI|P31137 protease hhob precursor (355 aa), FASTA scores: opt: 400, E(): 3.8e-14, (32.4% identity in 346 aa overlap). Also similar to Q50320 34 kDa PROTEIN PRECURSOR from Mycobacterium tuberculosis (361 aa), FASTA scores: opt: 1689, E(): 0, (70.7% identity in 362 aa overlap). Contains PS00135 Serine proteases, trypsin family, serine active site. Has a putative signal sequence at the N-terminus. BELONGS TO THE SERINE PROTEASE FAMILY.; mtb32a >NC_000962.3:151147-152214 Mycobacterium tuberculosis H37Rv, complete genome GATGAGCAATTCGCGCCGCCGCTCACTCAGGTGGTCATGGTTGCTGAGCGTGCTGGCTGCCGTCGGGCTG GGCCTGGCCACGGCGCCGGCCCAGGCGGCCCCGCCGGCCTTGTCGCAGGACCGGTTCGCCGACTTCCCCG CGCTGCCCCTCGACCCGTCCGCGATGGTCGCCCAAGTGGGGCCACAGGTGGTCAACATCAACACCAAACT GGGCTACAACAACGCCGTGGGCGCCGGGACCGGCATCGTCATCGATCCCAACGGTGTCGTGCTGACCAAC AACCACGTGATCGCGGGCGCCACCGACATCAATGCGTTCAGCGTCGGCTCCGGCCAAACCTACGGCGTCG ATGTGGTCGGGTATGACCGCACCCAGGATGTCGCGGTGCTGCAGCTGCGCGGTGCCGGTGGCCTGCCGTC GGCGGCGATCGGTGGCGGCGTCGCGGTTGGTGAGCCCGTCGTCGCGATGGGCAACAGCGGTGGGCAGGGC GGAACGCCCCGTGCGGTGCCTGGCAGGGTGGTCGCGCTCGGCCAAACCGTGCAGGCGTCGGATTCGCTGA CCGGTGCCGAAGAGACATTGAACGGGTTGATCCAGTTCGATGCCGCGATCCAGCCCGGTGATTCGGGCGG GCCCGTCGTCAACGGCCTAGGACAGGTGGTCGGTATGAACACGGCCGCGTCCGATAACTTCCAGCTGTCC CAGGGTGGGCAGGGATTCGCCATTCCGATCGGGCAGGCGATGGCGATCGCGGGCCAGATCCGATCGGGTG GGGGGTCACCCACCGTTCATATCGGGCCTACCGCCTTCCTCGGCTTGGGTGTTGTCGACAACAACGGCAA CGGCGCACGAGTCCAACGCGTGGTCGGGAGCGCTCCGGCGGCAAGTCTCGGCATCTCCACCGGCGACGTG ATCACCGCGGTCGACGGCGCTCCGATCAACTCGGCCACCGCGATGGCGGACGCGCTTAACGGGCATCATC CCGGTGACGTCATCTCGGTGACCTGGCAAACCAAGTCGGGCGGCACGCGTACAGGGAACGTGACATTGGC CGAGGGACCCCCGGCCTG >NP_214639.1 serine protease PepA [Mycobacterium tuberculosis H37Rv] MSNSRRRSLRWSWLLSVLAAVGLGLATAPAQAAPPALSQDRFADFPALPLDPSAMVAQVGPQVVNINTKL GYNNAVGAGTGIVIDPNGVVLTNNHVIAGATDINAFSVGSGQTYGVDVVGYDRTQDVAVLQLRGAGGLPS AAIGGGVAVGEPVVAMGNSGGQGGTPRAVPGRVVALGQTVQASDSLTGAEETLNGLIQFDAAIQPGDSGG PVVNGLGQVVGMNTAASDNFQLSQGGQGFAIPIGQAMAIAGQIRSGGGSPTVHIGPTAFLGLGVVDNNGN GARVQRVVGSAPAASLGISTGDVITAVDGAPINSATAMADALNGHHPGDVISVTWQTKSGGTRTGNVTLA EGPPA Protective antigen pepN Mycobacterium tuberculosis H37Rv 887403 57116994 Rv2467 AL123456 YP_177885 83332 2768985 2771570 + aminopeptidase PepN 4.68 87835.24 861 Also known as pepDLysyl aminopeptidase (LYS-AP) (alanine aminopeptidase) >NC_000962.3:2768985-2771570 Mycobacterium tuberculosis H37Rv, complete genome CGTGGCCCTTCCAAACCTCACGCGGGACCAAGCCGTCGAACGCGCCGCCCTGATAACCGTGGACAGCTAC CAGATCATTCTCGATGTGACCGACGGTAACGGCGCTCCCGGCGAACGCACCTTCCGGTCGACCACCACCG TGGTGTTCGACGCACTCCCCGGCGCCGACACGGTCATCGACATCTCCGCCCACACCGTGCGCCGCGCCAG CCTCAACGACCAAGACCTGGACGTCTCGGGATATGACGAGGCGGCCGGGATCCCGTTGCGCGGACTGGCC CAGCGCAACGTCGTCGTCGTCGACGCCGACTGCCACTACTCCAATACCGGCGAGGGCCTGCATCGGTTTG TCGATCCGGTGGACGGCGAGACCTACCTGTACTCGCAATTCGAAACCGCCGACGCCAAGCGCATGTTCGC CTGCTTCGACCAACCCGACCTCAAGGCCACGTTTGACGTGCGGGTGACCGCGCCCGCGCACTGGAAGGTG ATCTCCAACGGCGCGCCGCTGGCCGCGGCAAACGGCGTACACACCTTCGCCACTACCCCGCGGATGAGCA CCTATCTGGTGGCCTTGATCGCCGGACCATACGCGGCCTGGACGGACACTTACATCGACGACCACGGGGA AATCCCACTCGGCATCTATTGCCGGGCCTCGCTTGCCGAATACATGGACGCCGAGCGGCTGTTCACCCAA ACCAAGCAGGGATTCGGCTTCTACCACAAGCACTTTGGCCTGCCATACGCGTTCGGCAAGTACGACCAGC TCTTCGTCCCCGAATTCAACGCCGGCGCAATGGAAAACGCCGGCGCGGTGACCTTCTTGGAGGACTACGT CTTCCGCAGCAAGGTCACCCGGGCATCCTATGAGCGGCGCGCGGAGACCGTGCTGCACGAGATGGCCCAC ATGTGGTTCGGCGACCTGGTCACCATGACCTGGTGGGACGATCTGTGGCTGAACGAGTCCTTCGCCACCT TCGCCTCGGTGCTGTGCCAAAGCGAGGCCACCGAATTCACCGAGGCTTGGACGACGTTTGCGACCGTGGA GAAGTCTTGGGCGTATCGCCAAGACCAGCTGCCGTCGACGCACCCGATCGCCGCCGACATCCCCGACCTG GCCGCTGTCGAGGTGAACTTCGACGGGATCACCTACGCCAAGGGCGCCTCGGTGCTCAAACAGCTCGTTG CCTACGTCGGGCTGGAGCGCTTTCTGGCCGGCCTGCGTGACTACTTCCGCACGCACGCTTTTGGCAATGC CAGCTTTGACGATCTGCTGGCCGCGTTGGAAAAGGCCTCGGGCCGCGACCTGTCGAATTGGGGCGAGCAG TGGCTGAAGACGACCGGGCTCAACACCCTGCGACCAGATTTCGAGGTTGATGCCGAGGGCAGGTTCACCC GGTTCGCGGTGACACAGAGCGGTGCGGCACCCGGCGCAGGTGAGACCAGGGTGCATCGGTTGGCGGTGGG CATCTACGACGATGATGGTTCCAAGAGTTCCGGCAAGCTGGTCCGGGTGCACCGCGAGGAACTCGATGTC TCCGGTCCGATCACGAACGTCCCTGCGCTGGTTGGCGTTTCGCGCGGGAAACTGATTCTGGTCAACGACG ACGACCTGACCTACTGTTCGCTGCGGCTGGACGAGCGGTCGCTACAGACCGCGCTAGACCGCATCGCCGA CATCGCCGAGCCGCTGCCGCGCACGCTGGTGTGGTCGGCCGCCTGGGAAATGACCCGTGAAGCCGAACTG CGTGCCCGCGACTTCGTGTCACTGGTGTCCGGCGGCGTGCACGCAGAAACGGAGGTCGGGGTCGCGCAGC GGCTGCTGCTACAGGCGCAGACAGCGTTGGGTTGCTATGCCGAGCCCGGCTGGGCCCGGGAGCGGGGATG GCCGCAGTTCGCCGACCGGCTGCTGGAGTTGGCGCGCGAAGCCGAGCCTGGGTCGGATCATCAGCTGGCC TATATCAACTCGCTGTGTTCGTCGGTGTTGTCCCCCCGGCATGTGCAGACCCTAGGGGCGTTGCTCGAGG GTGAGCCCGCCGCATGTGGATTGGCAGGCTTAGCCGTCGACACCGACCTGCGCTGGCGGATCGTAACCGC GCTGGCCACCGCGGGCGCCATCGACGCCGACGGGCCGGAGACACCGAGAATCGACGCCGAGGTGCAGCGC GACCCGACTGCCGCCGGAAAGCGGCATGCCGCCCAGGCCCGCGCGGCGCGGCCACAGTTCGTCGTCAAGG ACGAGGCATTCACCACGGTGGTCGAGGACGACACCCTGGCCAACGCCACTGGCCGCGCGATGATCGCCGG CATTGCCGCACCCGGACAAGGCGAGCTGCTCAAGCCGTTCGCGCGACGCTACTTTCAGGCGATCCCCGGA GTATGGGCACGGCGATCCAGCGAAGTCGCGCAATCGGTGGTGATTGGCCTGTATCCGCACTGGGACATCA GCGAGCAGGGCATCACCGCCGCCGAGGAGTTCCTCAGCGACCCCGAGGTTCCGCCCGCATTGCGCCGGCT GGTGCTCGAGGGCCAGGCCGCGGTGCAGCGATCGTTGCGGGCCCGCAACTTCGACGCTGACGGCTA >YP_177885.1 aminopeptidase PepN [Mycobacterium tuberculosis H37Rv] MALPNLTRDQAVERAALITVDSYQIILDVTDGNGAPGERTFRSTTTVVFDALPGADTVIDISAHTVRRAS LNDQDLDVSGYDEAAGIPLRGLAQRNVVVVDADCHYSNTGEGLHRFVDPVDGETYLYSQFETADAKRMFA CFDQPDLKATFDVRVTAPAHWKVISNGAPLAAANGVHTFATTPRMSTYLVALIAGPYAAWTDTYIDDHGE IPLGIYCRASLAEYMDAERLFTQTKQGFGFYHKHFGLPYAFGKYDQLFVPEFNAGAMENAGAVTFLEDYV FRSKVTRASYERRAETVLHEMAHMWFGDLVTMTWWDDLWLNESFATFASVLCQSEATEFTEAWTTFATVE KSWAYRQDQLPSTHPIAADIPDLAAVEVNFDGITYAKGASVLKQLVAYVGLERFLAGLRDYFRTHAFGNA SFDDLLAALEKASGRDLSNWGEQWLKTTGLNTLRPDFEVDAEGRFTRFAVTQSGAAPGAGETRVHRLAVG IYDDDGSKSSGKLVRVHREELDVSGPITNVPALVGVSRGKLILVNDDDLTYCSLRLDERSLQTALDRIAD IAEPLPRTLVWSAAWEMTREAELRARDFVSLVSGGVHAETEVGVAQRLLLQAQTALGCYAEPGWARERGW PQFADRLLELAREAEPGSDHQLAYINSLCSSVLSPRHVQTLGALLEGEPAACGLAGLAVDTDLRWRIVTA LATAGAIDADGPETPRIDAEVQRDPTAAGKRHAAQARAARPQFVVKDEAFTTVVEDDTLANATGRAMIAG IAAPGQGELLKPFARRYFQAIPGVWARRSSEVAQSVVIGLYPHWDISEQGITAAEEFLSDPEVPPALRRL VLEGQAAVQRSLRARNFDADG Protective antigen This protein has been used to prepare a fusion protein for TB vaccine development [Ref6780:Junqueira-Kipnis et al., 2022]. pfkB Mycobacterium tuberculosis H37Rv 887491 15609166 Rv2029c AL123456 NP_216545 83332 2275404 2276423 - 6-phosphofructokinase PfkB 5.66 32325.93 339 phosphohexokinase (phosphofructokinase) >NC_000962.3:2275404-2276423 Mycobacterium tuberculosis H37Rv, complete genome TTCATGGCGAGGCTTCCGGGTTAACGATCGGGTGCCAAACGTATTGATCCTGCCCGACTTCGGTGGGTTC GGCCGCCAGCTCGAAGAACCTCTCCACATCGTCGCGATTGCAGGCCGCGGTGCCTGGCGTCAGCAGCATG GCTGCACCTGCCGCGTTTCCCAAGCGAACGGACTTGATGAGCGACCAGCCACGGCTGAGGCCCACGGTAA TCGCGGCCACCATCGCGTCGCCGGCGCCGACACCGCTAACCGCGGTCATCGGAATCGACGAAAATCGATG GCTCGCATGTCGTGTGGCCAATAGCGCGCCCTGAGATCCAAGCGAGACCACCACGACCTCGGCGCGCCCA CGGTCAATGAGTTCGTGTGCGGCGGCCAGTTGTTCGGGCTCGGTCAGCAGTTCGGATCCGACGCACTCGC GCAGTTCCCGCACGCTCGCCTTGAGAAGAAACACCCCGGACGAAATGTGCTGCAACCCGCCACCAGATGT ATCCAGGATCAGCGGAGTGCTCGATCGGCGGCAGATGTCGGCAACCCGCTGATAGTAGTCGGCAGCCACA CCTGGCGGCAGGCTGCCACTGGCCACCACAAAGGCGGCCGAAGCCGCCGCACCGCGCAGTTCGTCGAGGC ATTGCTCCTGCTCCGCGACGGTCAGCGACGGCCCCGGAAGCACGAAACGATACTGCTTGGCGGTCCTGGA CTCGTTGACCGTGAAGCTCTCCCGCGTCGAGGCCGCGATCGGAATGACGCGAAATGGCACTCCCGCATCA CCGAGCAGCGCCATCAGCAGGCTCCCGGTCGACCCGCCGGCCGGGAACAGTGCTGTCGAGCAACCGCCGA GGACATGCACAATGCGGGCGACATTGATACCGCCGCCGCCGGGATCGTAGCGAGGTGCGCCACAACGCAT TTTCTCGGTCGGGCGCACCACGTCGACGCTCGTCGTGATGTCCAAGGCGGGGTTCATGGTCAAAGTGATG ATTCGCGGCTTGCCTTCGTCCCACGCCGCTGGCTCCGTCA >NP_216545.1 6-phosphofructokinase PfkB [Mycobacterium tuberculosis H37Rv] MTEPAAWDEGKPRIITLTMNPALDITTSVDVVRPTEKMRCGAPRYDPGGGGINVARIVHVLGGCSTALFP AGGSTGSLLMALLGDAGVPFRVIPIAASTRESFTVNESRTAKQYRFVLPGPSLTVAEQEQCLDELRGAAA SAAFVVASGSLPPGVAADYYQRVADICRRSSTPLILDTSGGGLQHISSGVFLLKASVRELRECVGSELLT EPEQLAAAHELIDRGRAEVVVVSLGSQGALLATRHASHRFSSIPMTAVSGVGAGDAMVAAITVGLSRGWS LIKSVRLGNAAGAAMLLTPGTAACNRDDVERFFELAAEPTEVGQDQYVWHPIVNPEASP Protective antigen PhoP Mycobacterium tuberculosis H37Rv 888772 15607897 Rv0757 BX842574 NP_215271 3R0J 83332 851607 852350 ? two component system response transcriptional positive regulator PHOP 6.31 24835.35 247 Rv0757, (MTCY369.02), len: 247 aa. Possible phoP, two component system response phosphate regulon transcriptional regulator (see citations below), highly similar to various transcriptional regulators e.g. CAC32360.1|AL583945 putative two component system response regulator from Streptomyces coelicolor (271 aa); T45446 probable two-component response regulator from Mycobacterium leprae (253 aa); and similar to phoP proteins e.g. P13792|PHOP_BACSU alkaline phosphatase synthesis transcription regulatory protein from Bacillus subtilis (240 aa), FASTA scores: opt: 594, E(): 2.3e-33, (41.0% identity in 234 aa overlap); etc. Also highly similar to Rv3765c from Mycobacterium tuberculosis (234 aa), Rv1033c (257 aa), RV0903c|MTCY31.31c|Q10531 (236 aa), FASTA score: (45.4% identity in 229 aa overlap); MTCY10G2_16 and MTU88959_1. >gi|57116681:851607-852350 Mycobacterium tuberculosis H37Rv, complete genome AATGCGGAAAGGGGTTGATCTCGTGACGGCGGGAACCCCAGGCGAAAACACCACACCGGAGGCTCGTGTC CTCGTGGTCGATGATGAGGCCAACATCGTTGAACTGCTGTCGGTGAGCCTCAAGTTCCAGGGCTTTGAAG TCTACACCGCGACCAACGGGGCACAGGCGCTGGATCGGGCCCGGGAAACCCGGCCGGACGCGGTGATCCT CGATGTGATGATGCCCGGGATGGACGGCTTTGGGGTGCTGCGCCGGCTGCGCGCCGACGGCATCGATGCC CCGGCGTTGTTCCTGACGGCCCGTGACTCGCTACAGGACAAGATCGCGGGTCTGACCCTGGGTGGTGACG ACTATGTGACAAAGCCCTTCAGTTTGGAGGAGGTCGTGGCCAGGCTGCGGGTCATCCTGCGACGCGCGGG CAAGGGCAACAAGGAACCACGTAATGTTCGACTGACGTTCGCCGATATCGAGCTCGACGAGGAGACCCAC GAAGTGTGGAAGGCGGGCCAACCGGTGTCGCTGTCGCCCACCGAATTCACCCTGCTGCGCTATTTCGTGA TCAACGCGGGCACCGTGCTGAGCAAGCCTAAGATTCTCGACCACGTTTGGCGCTACGACTTCGGTGGTGA TGTCAACGTCGTCGAGTCCTACGTGTCGTATCTGCGCCGCAAGATCGACACTGGGGAGAAGCGGCTGCTG CACACGCTGCGCGGGGTGGGCTACGTACTGCGGGAGCCTCGATG >gi|15607897|ref|NP_215271.1| two component system response transcriptional positive regulator PHOP [Mycobacterium tuberculosis H37Rv] MRKGVDLVTAGTPGENTTPEARVLVVDDEANIVELLSVSLKFQGFEVYTATNGAQALDRARETRPDAVIL DVMMPGMDGFGVLRRLRADGIDAPALFLTARDSLQDKIAGLTLGGDDYVTKPFSLEEVVARLRVILRRAG KGNKEPRNVRLTFADIELDEETHEVWKAGQPVSLSPTEFTLLRYFVINAGTVLSKPKILDHVWRYDFGGD VNVVESYVSYLRRKIDTGEKRLLHTLRGVGYVLREPR Virmugen Study evaluated immunogenicity and protection against Mycobacterium tuberculosis in rhesus monkeys with an attenuated M. tuberculosis with a disrupted phoP gene (SO2) as a single-dose vaccine. SO2 treatment showed reduced average lung bacterial counts (1.0 and 1.2 log respectively, compared with 0.4 log for BCG) and significant protective effect by reduction in C-reactive protein levels, body weight loss, and decrease of erythrocyte-associated hematologic parameters (MCV, MCH, Hb, Ht) as markers of inflammatory infection, all relative to non-vaccinated controls [Ref1183:Verreck et al., 2009]. PPE18 Mycobacterium tuberculosis H37Rv VO_0010954 886073 57116841 Rv1196 AL123456 YP_177795 83332 1339348 1340523 + PPE family protein PPE18 4.81 36280.96 391 Rv1196, (MTCI364.08), len: 391 aa. PPE18 (alternate gene name: mtb39a). Member of the Mycobacterium tuberculosis PPE family of glycine-rich proteins, highly similar to others e.g. Y07P_MYCTU|Q11031 hypothetical 40.0 kDa protein cy02b10.25c (396 aa), FASTA scores: opt: 2124, E(): 0, (85.1% identity in 397 aa overlap). Note that expression of Rv1196 was demonstrated in lysates by immunodetection (see Dillon et al., 1999).; mtb39a >NC_000962.3:1339348-1340523 Mycobacterium tuberculosis H37Rv, complete genome AATGGTGGATTTCGGGGCGTTACCACCGGAGATCAACTCCGCGAGGATGTACGCCGGCCCGGGTTCGGCC TCGCTGGTGGCCGCGGCTCAGATGTGGGACAGCGTGGCGAGTGACCTGTTTTCGGCCGCGTCGGCGTTTC AGTCGGTGGTCTGGGGTCTGACGGTGGGGTCGTGGATAGGTTCGTCGGCGGGTCTGATGGTGGCGGCGGC CTCGCCGTATGTGGCGTGGATGAGCGTCACCGCGGGGCAGGCCGAGCTGACCGCCGCCCAGGTCCGGGTT GCTGCGGCGGCCTACGAGACGGCGTATGGGCTGACGGTGCCCCCGCCGGTGATCGCCGAGAACCGTGCTG AACTGATGATTCTGATAGCGACCAACCTCTTGGGGCAAAACACCCCGGCGATCGCGGTCAACGAGGCCGA ATACGGCGAGATGTGGGCCCAAGACGCCGCCGCGATGTTTGGCTACGCCGCGGCGACGGCGACGGCGACG GCGACGTTGCTGCCGTTCGAGGAGGCGCCGGAGATGACCAGCGCGGGTGGGCTCCTCGAGCAGGCCGCCG CGGTCGAGGAGGCCTCCGACACCGCCGCGGCGAACCAGTTGATGAACAATGTGCCCCAGGCGCTGCAACA GCTGGCCCAGCCCACGCAGGGCACCACGCCTTCTTCCAAGCTGGGTGGCCTGTGGAAGACGGTCTCGCCG CATCGGTCGCCGATCAGCAACATGGTGTCGATGGCCAACAACCACATGTCGATGACCAACTCGGGTGTGT CGATGACCAACACCTTGAGCTCGATGTTGAAGGGCTTTGCTCCGGCGGCGGCCGCCCAGGCCGTGCAAAC CGCGGCGCAAAACGGGGTCCGGGCGATGAGCTCGCTGGGCAGCTCGCTGGGTTCTTCGGGTCTGGGCGGT GGGGTGGCCGCCAACTTGGGTCGGGCGGCCTCGGTCGGTTCGTTGTCGGTGCCGCAGGCCTGGGCCGCGG CCAACCAGGCAGTCACCCCGGCGGCGCGGGCGCTGCCGCTGACCAGCCTGACCAGCGCCGCGGAAAGAGG GCCCGGGCAGATGCTGGGCGGGCTGCCGGTGGGGCAGATGGGCGCCAGGGCCGGTGGTGGGCTCAGTGGT GTGCTGCGTGTTCCGCCGCGACCCTATGTGATGCCGCATTCTCCGGCGGCCGGCTA >YP_177795.1 PPE family protein PPE18 [Mycobacterium tuberculosis H37Rv] MVDFGALPPEINSARMYAGPGSASLVAAAQMWDSVASDLFSAASAFQSVVWGLTVGSWIGSSAGLMVAAA SPYVAWMSVTAGQAELTAAQVRVAAAAYETAYGLTVPPPVIAENRAELMILIATNLLGQNTPAIAVNEAE YGEMWAQDAAAMFGYAAATATATATLLPFEEAPEMTSAGGLLEQAAAVEEASDTAAANQLMNNVPQALQQ LAQPTQGTTPSSKLGGLWKTVSPHRSPISNMVSMANNHMSMTNSGVSMTNTLSSMLKGFAPAAAAQAVQT AAQNGVRAMSSLGSSLGSSGLGGGVAANLGRAASVGSLSVPQAWAAANQAVTPAARALPLTSLTSAAERG PGQMLGGLPVGQMGARAGGGLSGVLRVPPRPYVMPHSPAAG Protective antigen PPE31 Mycobacterium tuberculosis H37Rv VO_0012372 885072 WP_003409179 Rv1807 AL123456 YP_177653 CDD:227938 1763 2048397 2049596 + PPE family protein 4.1 39253.11 475 PPE-repeat protein [Function unknown] >NC_000962.3:2048397-2049596 Mycobacterium tuberculosis H37Rv, complete genome ACTTGACTTCGCCACGCTACCGCCCGAAATCAACTCGGCGCGTATGTATTCCGGCGCGGGCTCGGCCCCG ATGCTGGCCGCAGCGTCAGCCTGGCACGGCTTGTCCGCAGAACTGCGCGCCAGCGCACTGTCATACAGCT CGGTGCTTTCGACGCTGACCGGTGAAGAATGGCACGGTCCGGCGTCGGCATCGATGACAGCCGCGGCCGC CCCCTACGTGGCCTGGATGAGCGTCACCGCCGTCCGGGCCGAGCAGGCCGGGGCACAGGCGGAGGCTGCC GCTGCAGCGTACGAAGCCGCGTTCGCAGCAACGGTGCCCCCGCCGGTCATCGAGGCCAACCGCGCCCAGC TCATGGCGCTGATCGCCACCAATGTGCTAGGCCAAAACGCCCCCGCGATCGCGGCCACCGAGGCCCAGTA CGCCGAAATGTGGTCCCAGGACGCGATGGCCATGTACGGCTACGCCGGCGCCTCGGCAGCCGCTACCCAG CTGACCCCGTTCACCGAGCCGGTGCAGACTACCAACGCGTCCGGCCTGGCGGCCCAGTCGGCTGCGATTG CCCACGCCACCGGCGCCTCGGCTGGTGCTCAGCAAACGACGCTGTCGCAGCTGATCGCCGCCATACCGTC TGTACTGCAAGGACTTTCGTCATCGACTGCAGCCACGTTCGCGTCGGGGCCGTCCGGATTGCTGGGCATT GTCGGGTCTGGATCTTCCTGGCTCGACAAACTCTGGGCGTTACTGGACCCCAACTCCAATTTCTGGAACA CGATAGCTTCGTCCGGACTGTTCTTGCCGAGTAACACGATTGCGCCCTTTTTGGGTCTACTCGGCGGCGT GGCAGCTGCGGATGCGGCCGGGGATGTGTTGGGAGAGGCCACCAGTGGCGGGCTCGGTGGCGCGCTGGTG GCGCCGCTTGGCTCAGCGGGCGGGCTAGGCGGCACTGTCGCGGCCGGCCTGGGCAACGCGGCCACCGTCG GAACCTTGTCGGTGCCGCCGAGCTGGACGGCGGCCGCACCACTAGCCAGCCCCTTGGGCTCCGCGTTGGG AGGCACACCGATGGTGGCACCGCCCCCAGCAGTGGCGGCCGGCATGCCCGGAATGCCTTTCGGCACCATG GGCGGTCAAGGCTTCGGGCGTGCCGTGCCCCAGTATGGCTTCCGCCCCAACTTCGTCGCACGACCGCCCG CCGCCGGGTG >WP_003409179.1 MULTISPECIES: PPE family protein [Mycobacterium] MTAALDFATLPPEINSARMYSGAGSAPMLAAASAWHGLSAELRASALSYSSVLSTLTGEEWHGPASASMT AAAAPYVAWMSVTAVRAEQAGAQAEAAAAAYEAAFAATVPPPVIEANRAQLMALIATNVLGQNAPAIAAT EAQYAEMWSQDAMAMYGYAGASAAATQLTPFTEPVQTTNASGLAAQSAAIAHATGASAGAQQTTLSQLIA AIPSVLQGLSSSTAATSASGPSGLLGILGSGSSWLDKLWALLDPNSNFWNTIASSGLFLPSNTIAPFLGL LGGVAAADAAGDVLGEATSGGLGGALVAPLGSAGGLGGTVAAGLGNAATVGTLSVPPSWTAAAPLASPLG SALGGTPMVAPPPAVAAGMPGMPFGTMGGQGFGRAVPQYGFRPNFVARPPAAG Protective antigen A selection of previously identified protective Mycobacterium tuberculosis DNA vaccines were re-formulated as proteins and administered with a Th1-inducing adjuvant to help stimulate the relevant immune responses necessary for protection. One candidate (Rv1806-1807) induced protection in the guinea pig aerosol infection model 30 days post-challenge on the basis of reducing the bacterial burden of M. tuberculosis in the lungs [Ref329:Vipond et al., 2006]. PPE42 Mycobacterium tuberculosis H37Rv YP_177893 CDD:307116 CDD:279771 CDD:311933 83332 ? PPE family protein PPE42 4.26 57135.65 663 PPE family; pfam00823 >YP_177893.1 PPE family protein PPE42 [Mycobacterium tuberculosis H37Rv] MNFAVLPPEVNSARIFAGAGLGPMLAAASAWDGLAEELHAAAGSFASVTTGLAGDAWHGPASLAMTRAAS PYVGWLNTAAGQAAQAAGQARLAASAFEATLAATVSPAMVAANRTRLASLVAANLLGQNAPAIAAAEAEY EQIWAQDVAAMFGYHSAASAVATQLAPIQEGLQQQLQNVLAQLASGNLGSGNVGVGNIGNDNIGNANIGF GNRGDANIGIGNIGDRNLGIGNTGNWNIGIGITGNGQIGFGKPANPDVLVVGNGGPGVTALVMGGTDSLL PLPNIPLLEYAARFITPVHPGYTATFLETPSQFFPFTGLNSLTYDVSVAQGVTNLHTAIMAQLAAGNEVV VFGTSQSATIATFEMRYLQSLPAHLRPGLDELSFTLTGNPNRPDGGILTRFGFSIPQLGFTLSGATPADA YPTVDYAFQYDGVNDFPKYPLNVFATANAIAGILFLHSGLIALPPDLASGVVQPVSSPDVLTTYILLPSQ DLPLLVPLRAIPLLGNPLADLIQPDLRVLVELGYDRTAHQDVPSPFGLFPDVDWAEVAADLQQGAVQGVN DALSGLGLPPPWQPALPRLF Protective antigen ID93/GLA-SE protects against TB and MDR-TB in animals. PPE44 Mycobacterium tuberculosis H37Rv 888456 57117024 Rv2770c AL123456 YP_177677 83332 3079308 3080456 - PPE family protein PPE44 4.44 33995.28 382 Member of the Mycobacterium tuberculosis PPE protein family >NC_000962.3:3079308-3080456 Mycobacterium tuberculosis H37Rv, complete genome ATCAGAAGAGGCCGTGCTTGGGCATAACAATCGGCTTGACTCCGTACCGTGGTCCGGAGTCGGCACCAAC ACTGTTGGCGGCTACGACCATTCCAGGGGCAGGCGGCATCACTGCGATCGGGCCGTCCTCCTCGGGAACT GCCCAGCCTGTGCCATCCAAGGCCGCGCCGGCTGCCGTCGCCGGCGCTGCAGTAGACCAGCTTGCCGGCA CCGACAGGCGACCAACCACGGACGCATTGCCCAAATCGGCGGTCAGCGCTGTTCCGCCGACGCCCGCTGG GGCAACCGCGTGTGCCACCGCGGCTGCCGCGCCGCCACCTGGAGCGGCTCCGCCAACGGTTCCCATAGCA TCGGCAAGAAGCGTCATATTGCCAATGGCGGCCGTGGCAAAGTCTGCCACGCCACCCAGGCCGTGAAACG CGGATTCTACGAACAGCGGAACATCGAGATTGAGGAACTGCCTGACCGCCTCCAACCCGGTATCGGCCGC GGACATCACCGGGGAGGCGAAGCTCAGGACAGCGTCGGCGACGTCGCTGATCAGGTGGCTCAGACCCACC TGGCGCGCGAAAGCGGATGCGCCGGCTTGGCCAACAGCGGCGGCTTGGTGTGCGAGCCCGGCCGGATTGG TGATGTGCGACGGCCTGGTCAGCGGGTTCAGTCTTGCGGCGACCGCGGATGCGGCCGCATAGCCGTACAT GGCCGAAGCGTCTTGGGCCCACATTTCGCCATAGCGTGCCTCGGTAGCCGCGATGGCCGACACGTTTTGC CCAAGGATGTTGGTCGCTGTCAGTTCAGCCAACAGGGCTCTGTTGGCAACCACCTCGGCCGGGGGCACTG TCAGCGCAAACGCCGTTTCAAAGGCGGCCGCAGACGCCATGGCCTGTGCCGCCGCGAGCGCCGAGGATTC AGCGGTGCAGGTCAACCAGACCAAATAGGGCTGCACCGCGGCGGCCATCGACAACGATGCGGGACCCATC CAGTGCTCGGTGCTCAGCCGCGTGATGACCGACCCGACGGAGGACGCAGCTGTGCTCACCTCGACAGCTA TGCCGTTCCACGCAGCCGCAGCCGCCAGCAGGTCTGCCGCGCCCGCGCCGCCATACATGCGCGCAGAATT GACCTCCGGAGGTAGAGCTCCAAAATCCA >YP_177677.1 PPE family protein PPE44 [Mycobacterium tuberculosis H37Rv] MDFGALPPEVNSARMYGGAGAADLLAAAAAWNGIAVEVSTAASSVGSVITRLSTEHWMGPASLSMAAAVQ PYLVWLTCTAESSALAAAQAMASAAAFETAFALTVPPAEVVANRALLAELTATNILGQNVSAIAATEARY GEMWAQDASAMYGYAAASAVAARLNPLTRPSHITNPAGLAHQAAAVGQAGASAFARQVGLSHLISDVADA VLSFASPVMSAADTGLEAVRQFLNLDVPLFVESAFHGLGGVADFATAAIGNMTLLADAMGTVGGAAPGGG AAAAVAHAVAPAGVGGTALTADLGNASVVGRLSVPASWSTAAPATAAGAALDGTGWAVPEEDGPIAVMPP APGMVVAANSVGADSGPRYGVKPIVMPKHGLF Protective antigen PPE68 Mycobacterium tuberculosis H37Rv 886201 57117164 Rv3873 AL123456 YP_178022 83332 4351074 4352180 + PPE family protein PPE68 3.99 36038.85 368 Member of the Mycobacterium tuberculosis PPE family. A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004 PMID:14766927). Predicted possible vaccine candidate (See Zvi et al., 2008). >NC_000962.3:4351074-4352180 Mycobacterium tuberculosis H37Rv, complete genome CATGCTGTGGCACGCAATGCCACCGGAGCTAAATACCGCACGGCTGATGGCCGGCGCGGGTCCGGCTCCA ATGCTTGCGGCGGCCGCGGGATGGCAGACGCTTTCGGCGGCTCTGGACGCTCAGGCCGTCGAGTTGACCG CGCGCCTGAACTCTCTGGGAGAAGCCTGGACTGGAGGTGGCAGCGACAAGGCGCTTGCGGCTGCAACGCC GATGGTGGTCTGGCTACAAACCGCGTCAACACAGGCCAAGACCCGTGCGATGCAGGCGACGGCGCAAGCC GCGGCATACACCCAGGCCATGGCCACGACGCCGTCGCTGCCGGAGATCGCCGCCAACCACATCACCCAGG CCGTCCTTACGGCCACCAACTTCTTCGGTATCAACACGATCCCGATCGCGTTGACCGAGATGGATTATTT CATCCGTATGTGGAACCAGGCAGCCCTGGCAATGGAGGTCTACCAGGCCGAGACCGCGGTTAACACGCTT TTCGAGAAGCTCGAGCCGATGGCGTCGATCCTTGATCCCGGCGCGAGCCAGAGCACGACGAACCCGATCT TCGGAATGCCCTCCCCTGGCAGCTCAACACCGGTTGGCCAGTTGCCGCCGGCGGCTACCCAGACCCTCGG CCAACTGGGTGAGATGAGCGGCCCGATGCAGCAGCTGACCCAGCCGCTGCAGCAGGTGACGTCGTTGTTC AGCCAGGTGGGCGGCACCGGCGGCGGCAACCCAGCCGACGAGGAAGCCGCGCAGATGGGCCTGCTCGGCA CCAGTCCGCTGTCGAACCATCCGCTGGCTGGTGGATCAGGCCCCAGCGCGGGCGCGGGCCTGCTGCGCGC GGAGTCGCTACCTGGCGCAGGTGGGTCGTTGACCCGCACGCCGCTGATGTCTCAGCTGATCGAAAAGCCG GTTGCCCCCTCGGTGATGCCGGCGGCTGCTGCCGGATCGTCGGCGACGGGTGGCGCCGCTCCGGTGGGTG CGGGAGCGATGGGCCAGGGTGCGCAATCCGGCGGCTCCACCAGGCCGGGTCTGGTCGCGCCGGCACCGCT CGCGCAGGAGCGTGAAGAAGACGACGAGGACGACTGGGACGAAGAGGACGACTGGTG >YP_178022.1 PPE family protein PPE68 [Mycobacterium tuberculosis H37Rv] MLWHAMPPELNTARLMAGAGPAPMLAAAAGWQTLSAALDAQAVELTARLNSLGEAWTGGGSDKALAAATP MVVWLQTASTQAKTRAMQATAQAAAYTQAMATTPSLPEIAANHITQAVLTATNFFGINTIPIALTEMDYF IRMWNQAALAMEVYQAETAVNTLFEKLEPMASILDPGASQSTTNPIFGMPSPGSSTPVGQLPPAATQTLG QLGEMSGPMQQLTQPLQQVTSLFSQVGGTGGGNPADEEAAQMGLLGTSPLSNHPLAGGSGPSAGAGLLRA ESLPGAGGSLTRTPLMSQLIEKPVAPSVMPAAAAGSSATGGAAPVGAGAMGQGAQSGGSTRPGLVAPAPL AQEREEDDEDDWDEEDDW Protective antigen proC Mycobacterium tuberculosis H37Rv 887256 15607641 Rv0500 BX842573 NP_215014 83332 590082 590969 ? pyrroline-5-carboxylate reductase 4.42 26600.99 295 catalyzes the formation of L-proline from pyrroline-5-carboxylate >gi|57116681:590082-590969 Mycobacterium tuberculosis H37Rv, complete genome GATGCTTTTCGGCATGGCAAGGATCGCGATTATCGGCGGCGGCAGCATCGGTGAGGCATTGCTGTCGGGT CTGCTGCGGGCGGGCCGGCAGGTCAAAGACCTGGTAGTGGCCGAGCGGATGCCCGATCGCGCCAACTACC TGGCGCAGACCTATTCGGTGTTGGTGACGTCGGCGGCCGACGCGGTGGAGAACGCGACGTTCGTCGTCGT CGCGGTCAAACCAGCCGACGTCGAGCCGGTGATCGCGGATCTGGCGAACGCGACTGCGGCGGCCGAAAAC GACAGTGCTGAGCAGGTGTTCGTCACCGTGGTAGCGGGCATCACGATCGCGTATTTCGAATCCAAGCTAC CGGCTGGGACGCCAGTGGTGCGTGCGATGCCGAACGCGGCGGCATTGGTGGGAGCGGGGGTTACAGCGCT GGCCAAAGGCCGCTTTGTCACCCCGCAACAGCTTGAGGAGGTCTCGGCCTTGTTCGACGCGGTCGGCGGC GTGCTGACCGTTCCGGAATCGCAGTTGGACGCGGTGACCGCGGTGTCCGGCTCGGGTCCGGCCTATTTCT TTCTGCTGGTCGAGGCCCTGGTGGATGCCGGAGTCGGGGTGGGCTTGAGCCGTCAGGTGGCCACCGATCT CGCCGCGCAGACAATGGCTGGCTCAGCGGCGATGCTGCTGGAGCGGATGGAGCAAGACCAGGGTGGCGCC AATGGCGAGCTGATGGGGCTGCGCGTGGACCTTACCGCATCACGGCTGCGCGCCGCGGTTACCTCGCCGG GCGGTACGACCGCCGCTGCGCTGCGGGAACTCGAACGCGGCGGGTTTCGGATGGCTGTCGACGCGGCGGT TCAAGCCGCCAAAAGCCGCTCTGAGCAGCTCAGAATTACACCGGAATG >gi|15607641|ref|NP_215014.1| pyrroline-5-carboxylate reductase [Mycobacterium tuberculosis H37Rv] MLFGMARIAIIGGGSIGEALLSGLLRAGRQVKDLVVAERMPDRANYLAQTYSVLVTSAADAVENATFVVV AVKPADVEPVIADLANATAAAENDSAEQVFVTVVAGITIAYFESKLPAGTPVVRAMPNAAALVGAGVTAL AKGRFVTPQQLEEVSALFDAVGGVLTVPESQLDAVTAVSGSGPAYFFLLVEALVDAGVGVGLSRQVATDL AAQTMAGSAAMLLERMEQDQGGANGELMGLRVDLTASRLRAAVTSPGGTTAAALRELERGGFRMAVDAAV QAAKSRSEQLRITPE Virmugen A proC mutant is highly attenuated in mice and induces significant protection from challenge with wild type M. tuberculosis that is equal to the protection induced by the current vaccine BCG [Ref1672:Smith et al., 2001]. PTCD1 Homo sapiens 26024 38492358 AC004922 NP_056360 9606 7 93650104 93673129 - pentatricopeptide repeat domain 1 8.78 75381.12 700 Also known as KIAA0632 >gi|157734172:93650104-93673129 Homo sapiens chromosome 7, alternate assembly HuRef, whole genome shotgun sequence TGAAACAGGGTCTTGCTCTGTTCCACAGGCTGGAGTGCAGTGGTGCAATGATGGCTCACTGCAGCCTCAA CCTTCTGGGCTCAAGGGATCCTCTCACCTCAACCTCCTGAGTAGCTGTGACTACAGGTGTGCACCACCAC ACCCAGCTAATTTTTAAATTTTTTGTGGAGATGGGCCCTTGGCCCAGGCTGGTTTCTTAACTCAAATGAT CCTCTGGCGTTGGCTTCCAAAAGTGCTGGGATTACAGGTGTGAGGCACCTTGCCTGGCCTAAGTACTTTT TTTTTTTGTTCCTCCTCCAAGGACTGTCCCGTTGCAATACTGAATGCCTTTTTTTTTTTGAGATGGAGTT TCGCTGTCACCCAGGCTGGAGTCCAGTGGCACGACCTCAGGTCACTACAACCTCCGCCTCCTGGGTTCAA GTGATTCTCCTGCCTCAGCCTCCTAAGTAGCTGGGATTACAGCTGCTACCACGCCCGAGTAATTTTTGTA TTTTTAGTAGAGACGGGGTTTTACCATGTTGGCCGGGCTGGTCCTGAACTCCTGACCTCAGGTGATCCGC CTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCACCCGGCCTGAATGCTTTTTTTG ATCAAGGGTGGGGAAGGTTTTTAGACCATGGCCTGATGCTCTTTCCCCATCTTTTTGACAGAACTCTATG AATATTGTATTAAAGAAGGCTATGCAGACAAAAACCTGATTGCAAAATGGAAAAAGCAAGGATATGAGAA CTTGTGCTGCCTGCGGTGCATTCAGACACGGGACACCAACTTCGGGACGAACTGCATCTGCCGCGTGCCC AAAAGCAAGCTGGAAGTGGTAATGTCTGACACTCAAGCTTGGTGTTGTTTTCAGCTCAAAATTCTGCCTT AGTCGACTGCAAGGGATCTTATGTTATTTGGTTGGGCTGGAATGTCGTTTTGTCCCTGGATGTCCTGCTG GCTCTCCCTCGTGGGAGTGGGTCCCTGTATTCAGGAATCCATGTGAGGCAGCGTGTGGCTGTGTGTTTGT TAGGTCTGGGGTCAATCTCAACTCCACTTTTGGGCAAATTACTGAACCCCTTTCCTCACTTAGGAAATGG TGGTGGGGAGCCCTAATCCCAAGGTGGTGGCAGGGTGACATCAGGGAAGGACAACCAGTGAGTTCCTGTC CCTTTCAGTCCTCACAGTGATACTTTAACATTACCATCACTATCTTTCCCGCCCCCCCAAGACGGAGTCT TGCTTTGTCGCCCAGGCTGGAGTGCAGTGATGCCATCTTGGCTCACTGCAACCTCCACCTCCCGGGTTCA AGCGGTTCTCCTGCCTCATCCTCCTGAGTAGCTGGGACTACAGGCATGCACCACCACGCCTGGCTAATTT TGTATTTTTAGTAGAGACGGGGTTTCTCAATGTTGCTCAGGCTGGTCTCGAACTCCCGACCTCAGGTGAT CCACCCGCCTCAGCCTCCCAAAGTGCCGGGATTACAGGCATGAGCTACTGCGCCCAGCCCACTGTGTTTC TTCTTTCAGAACTTTCTGTTCCAAGTACCACTCCTAGGCCAGGCGCCTCAGGAGTGGTGCTGAGGGACAG GAGCTGAGCTCCATCCGGTTCCAAGCCCCTCTGAAAGGCAGCTGTGGCCCGAGGCAGAGTCACAGCAGTG GCCGCTCAGTGCAGCCCCTCAGCACAGTTGTTGGCCCCACCGCCCATCTGCCAGCCTGGCCTTCTTTTCC CCAGCTCCACACAGGGGCCTCTTCACATGAGTCCCCTTTATGCTGCAGTGAGACTGACAATGGAGAGCTG TGGCTGGATGCTGAGGACTTGGGTACTGAGCAGTGGGGGCGAGCAAGGGCCGCCTCCCCGACGGAAGACT GGGGTGTTGGTTTTCTGATGGAGGCAGGGACAGGTGGCAGAGTGGCAGAAGCCCCCTTCCTCTGAGTGCC CAGATTCCACAAGGAGTCCAGGTCAGGGGCTGGTGGGCTGGGGTGCTGCTTCAGCCCTGTGGCCAAAACA GCCCGCTGTAGACTTACCTGGGTTTCACCAGAGGATCCTGTTTACTTAAACTATGGTCTTGTTCTTCCAA AAAAAGAACTTGAAAAAGTAAAATAAACACAAGCCACTGTTCTAGGGCTTTCTGGCTGGCCAAGTGTTGG GCCGCACCAGGCCTGGGGGACAAGACCCTTCTTTCTGGGGGGCTCTGGGCCAGAAGGGTGTGACTCAAGT GCATAGTCTCCTGCCCTCTTCCCCCACCAGAGTGTACCAGCCCAGAGGCCCCCCTGAAGTCCCCAAACAG TAGCAGAGCCACGTCTGCTCATCGCAGGGTCTGTCATGCTCACTTAGCAGAATAACGAGACTCATTCACA CCGATTTCTTTTTCTTGATTGGCAAACGTGTGTTGGATTTGCAGAACATATTATAAATAGACATACAGAT GTAACCTCGGTGTCAACAGCAGCTGGTTCTACCTTCATGAGGGAGCCAAATTTTCCCTGTCCAGAGCTGT CAAGGAAGGGTTTCTGAGGTGTGTCCCTATATGGCATGGTGGCAGGTCCTTCGTGGGAGGGTTGCCACAT ATGTGAGTGTGCAGGGGCGAGCGTGGCGCAGTGGCATCGTCTCACTGTCCTCCTCCGTTGCAGGGCCGCA TCATCGAGTGCACACACTGTGGCTGTCGTGGCTGCTCTGGCTGAGGCTGGCGCGCTCCACCCTGGACTCT GGACTTCGCAGGTTCCTGCCTGTCACGCCACCCCCTTCCTGGGAGCAGCGAGCAGTGCCCCAGGCCCGAG TTGGAGCACGGTCTCTATGGGGAAGGCTTCGCTGTCTATCAGCTGTGATTTGTAAAAATAAAATCTTTAA ATCTCTCGAGCCCCACGTCTCTTCTTTCAGAGCATCGGCCTATGGAACCCGGCGGGGCGGCCCAGGCCCC AGGGACCAGATGCCCCAGCCCCCTTGTGGTGTGTGAGGTGACACACAAAGGTAGCTGGAGCTGGAAGTCC CGTGAAGGTGACACGCAAAGGTGGCTGGAGCTGCACTTGGACCTGCTGGGAGCACAGGCACCTTGGGCCT AGTGTGTGTCCTCACCAACACCTGTGACACGCTGCGGCTGTTCCTCAGGGCCTGGCTCTTCCCCCAGGCA GGAGGTGACACCAGCTCACTTGTCCTGGGGCTCCCACAGAGCACTGGGGGCCGAGCACATTGTTCCAGCT GTGCTCCCATCACCTGCCCCCAAGGGCACATCCGTCATCAGCCTCCTTGCCGGTGTCCTGGTCCCCCTGG GGCTTGGTCCGGAACTTCTGCCAGGGGTGCGGGGTTTCCTCTGCGGGCATCACTGTCAGCCACTGCTTGT AATAGGCTCGGAAGCCGTCAATCTTCTCCAGGTAGGTGTTCTTCCCTTGGTACTAGAATTAGAAAAGTGA GGCTAGAATCACTCCTGTTACCTACAGCTAATCCCAAACCTCGGCAGCTGGCTCAGGCCTCAGGGAAGCT GGTGGCTGCCATTTTCCCTTCCAGGTGGCTGCAGAGGACAGGGCAGAAAAGCTGTGAACACGCACTATGG GACTAGCTTACTTCAGAGAAACAGGTCCCCGCTGGGTACCCTGGGGCAGGCCCAGAGCCATCTGCCCAAT GGCGTTCTTCCCAAATAGGCACCACTGTGCTCCTCCATCCGCAGTTGCCACCCCAGGCCCCCAAGCCAGA ATCACTGGGTCCCACCAGCTCTGCCTCCACAGCCTGCCGAGCCCCTCCCCATCAAAGCCACCTCCCTCTG ACCATTATCTCAACACTGCCAAGATAGGCTCCATGAGATATGGGGAAAAAAGCAAAGACACACCAGACAC AAACTGAGAGCTGGTAGGGGAATATACCAAAAAAGCCAAAAAGTGCTATCCAACATACAGCTACTACCAC ACAATGACAAAAGGTTTGGCCGGGTGCGGTGGCTCACGTCTGTAATCCCAGCACTTTGAGAGGCCGAGGT GGGCAGATCACTTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAGCATGGTGAAACTCCATCTCTACTAAA AATACTGGATGTGGCGGTGCATGCCTGTAGCCCCAGCTACCTGGGAGGTTAAGGCAGGAGAATGGCTTGA ACCCGGGAGGCAGAGGTTGCAGTGAGCTGAGATCACGCCATTGCACTCCAGCCTGGGCGACAGAGCAAGA CTCCATCTCAAAAAAAAACAAAAACAAAAAAAGGTTTGACTCAAAAGACTGGACAATTCTAAACCTTAAG GTGCCGGATAGAGCCTGAAAGCACAGAAAGCAAAACTCAACAGAATCACGAATATAAACAAGTCCACCAT CATGAGGGAATATGAGTACAGATGCTCCTGACAGCAGGGTTCCGCCCCCCAAAAACCCATCGTAAATCAA AAATCAATCTGGCTGTGTGTGCTGGCTCACTCCTATAATCCCAGTGCTTTGGGAAGCCGAGGCAGGCGGA TTACATGAGCTCAGGAGTTCCAGACCAGTTTGGGCAATATAGTGAAACCCTATCTAAAAGATTAGCTGGG TACAGTGGTACATGCCTGTAGTCCCAGCTCCTCAGGTGGCTAAGGTGGGAGGACTTCTTAAGCCTGGGAG GTCAAGGCTACAGTGAGCCATAATCGTGCCACTGCACTCCAGCCTGGGCAACAGAGTGATTCCCGTCTCT TTAAAAAAAAAAAAAAAAAAGAAAAAAGAAAATGCGGCACGGCATGGGGGCTCACAACTATAATCCCAGC ACTTTGGGAGGCTGGGGTGGGTGGATCACGAGGTCGGGAGTTCAAGACCAGCCTGGCCAAGATGGTGAAA CCCCGTCTCTACTAAAAAAAAAAAAAAAACCACAAAAATTAGCCTGGTGTGGTGGCAGGCTCCTGTAATC CCAGCAACTCGGGAGGCTGAGGCAGGAAAATCACTTGAACCTGGGCGGCAGAGGTTGCAGTGAGGCCAGA TCGCATCACTGCACTCCAGCCTGGGTGACAGAGTGAGACTCCATCTCATAAAAAACAAAAGGAAAATGCA TCTATTGCTGGCAACACAGCAGAGGGTCCTGACTTTATAATTATGCAAAAGTGATATACATTCAGTAGAA ACTAACCCCGCACAAAGTCGATCCTTACAGAGTTACATCCTGATAAAACCATTGCAAAGTTGAAAAATCC TGTGTAGAATCATCTTTAAGTCAGGGACATCTGTCTGTGACTTAAATATGATATGCTTTAGGTCTATGAT AGTCTTGTGACTGTCGAGTTGAACCCCTAGTGAGCCCTACGCCAGAGGGAAGAGACAATCCACTAGGAAG ATATGCACAACATGTGATCAACAAAAAACCAGTACCCAGAATATATAAAAAACCCTGGAATCAATGAGAA AAGGCTTGCAACCAACAGTTCAAAGCACAGACACAGTTCAAAAATGCCCAGCTATGCCTTCAGCCAGACA CAATTTCCATTGCTGTCCCACCTATTTGTAGGAAGCCCTCAAAATGGCATTCAGGCGTGACACCTGCACG GCGTTCCTGACCCCGCACCCTCTAGCTGTGTGTGTCCCTGCCCATTTACTTGTTTCCGCCCCATCCGGGC TGTGCTTCAAGCCATAGTGGCTCTCGATGCTCCGGAGATGCTCAGCACAGAGTGTGATTCGATGGACCCA AAAGCCAAACCCCAGAGCCTGATAGCCTCAGAACAGCAGGGAGAGGCTGTGGCCGTGACAAAGCAGATTC CATGACTCTGCCCTGCTCCTGCTGATACAGGAGGTTGAAAGAAATTATTTAGGCAGATAATAAGGGCAAC AGAGTCCTTGGCGAAATTTCCCTTTTAATAAAAAGCAGCCCCCAAATAATTTCTTTTCTAACAAAGAGCA GCCTGAAAAATCGAGCTGCAGACATAGATAAGCAAACTGGAAGCTTGCAGGGGTAAATGCTGGCGGCTGT GCCAACAGAAAAGGGCTACCTGGGAGCCAGATATGTTCAACGTGGGGGCCCCATCTTCCCTTCTTTGTTG CCATGTGTACAGTAAAGAACCAGGCAACATGGCGATGGCCACCTGTCTGCATAATAAAAGGTTAGGGTGG GGCACCAGCTTTTATACATGCTATGTAAATGGCACACCTGGTCCAACTAATCTTTTGCGTCCTCTGTAAA TCAGACACCACCTCCCCAAGCTCATCTACAAAACCTCTGCATTACACTATGGAAGTGGCAACCCACTTCT CTGGCACCTCTCTCTGCAAGGAGAGAGCTCTTCTCTTTTTTTTTTTTTCACCACCACACCGGGCGAGAGC TCTTCTTTCTTTCGCCTATTAAACTTCAGCTCTTAACCTCTTTTTTTTTTTTTTGAGACCAAGTCTCCAT CTATCGCCCAGGCTGGAGTGCAGTGGCGCGATCTTGGCTCACAACAAATCTCAGGTATTCACCCCAGACA ATGACACCACTTCACTGCCTGCCCAGTCCCTGAGCAAGCGACAACCTGTTTGGAGTACCCAGCAACGTGC AGGTGCTGGGGACCCAGATGAGCACAGCAGACAGCCTCTGCCTCCAGAAGCCTGATACAGGGAAGGCCAG GGCTGCCCATGAGCACATGATCCAAGACTGTAGGACACGGCATAAAGGAACAGAGTGGGAAGGGGGAGGG GAGACTTTCATCCCAGGTCAGAGCGGCAGCCAGCTGGGCATGTCTGAGGGCCACTTAAGCAGAGACCTCC AGGAAAAGTGGGTGCCACCTGAGGCAGCGGGAACACCAATCCCGAAGCAGGCAAGAGGTTGGTGGCCTCT GCGAACTGACAGAAGGCCACTGGGACAGACAGAGAAAGCAAACGCAAGGCTGAGACATGAGATGAGCCAC GCAGACAGGGACTCGCAGGCTGTGGTCTACAGACTTGGGTACTGCTCCTAAGGGAAGCTGCTGGACAGTT TCAAGCATGGCAACACAATCCCAAGGGCCAGAACCGGGGTTGGGTGGTGGGGGGAGGGGGTAGCAATGGT GAAGGAGCTGATGAGCTTTTGAGCTTGGGGCACACACCCGGTCAAAGGTGGGAGGGTACTGGGCTGCAAA CTCCAGCTGGCGGATGACCACTTCGTTCACCGGGACCCTGTTCTGCTTCATGTCCTTCAAGATGCTGATG AGATAGGTGTAGTTCAGCTTCCTGATGGCCGCGTTGATGAGGGCACTGTAGATGTGAGTGTTGGGGGTCA CCTGGGACTTCTAGAACACAGGGACATCGTAGAATCAAGATGATGGCAGCCATTGGGACCCAGCAGCTCC CACCCTCTGTCAATCAAGCTGGCTCCCGGGACCAGCGGCCAGGGCCAGCAAGGCAAACTTGGCAGCAACA ATGTGCTGAATATCCCTCTCCAGCAGGAAAGCCAAGCCCAGCCCCTCTGCTCAGCCCTAAAGAAGCTGCG GAAAAGGACGCTCGTGCTGCTCTCAGACACCGACTGACTCAGGCAGAAAATGATGTGGGAGACACCACCT CCTTGCTCCCTGGGGTGGGCTTCCTGTGACGCACGGTCACCAGGTGGTGGTTGGCCTAGAAAAGTGCAAG CCTTGGCTGGGTGAGCCCGGTAGGGTCCAGGAAAACAGCCAGAGGATGATGGACCTGGGGACACCGACAA CCCAGGACGCAGCCTCTGCTGTGGTCGGATGAAATCCACGCAGCACTTCCAATGGGGGTTAGGTGGGAGG CACAGACCTCGGGGGCTTTGAAAACTGCCCACGGCCCCTCAGGAATTCTGAGAATCCCTGCCATACCTCT AGAGTGTTGGGGCCGAGGCCTGTGCCCAGCCCTGTGTCAACCCACGGCCACCTTCTTCCGGATGGGAACA TCCGTGTGTCTCTACGCCCAGCCCTGAAGCACTCACTGGGGTCTGCTTCCCCCAAAATGGGACCAAAGAC CAGAAGGTCATTGACCCCACTCTCTTTGCCCCATGCACAGTTCATCCTCTCCAGGTGGGGGGTCTGCAGA CCCCTCAAACTTTCCTCCTGAGCTGCAGAGTGCTCCTGAGCACCATGGGTGTCCTGCCCAGGCCCGAGTC CACTCACCTTCATGTCTGTGAGAAGCTGTAGACCGTCCTTCGGCCTGTGGCACCCGATGGCCAGGTTGCA GAATGTCTGCAGGTTGGGGACGAGGCCCCTCTTTGCCAGGACCGGCAACAGCGCCTTGGCCCCCTCCAGG TCTCCCAGCTTGCTCTTCTTTCTCACCAGCGTGTTAAAGAATGTCAGGTCGGCCTCTACCTGGTGCTCAT CCAGGAGGGCCAGCAGCAAGGACTCTGCAGGACTCCCGGACTCCACCACCTCGGCCAGTAGCGTGAGGGT CCTGATGTCGGGCTGCTGCCTGTGCTCTGCCATCTTGCTCAGGAAGCCCTCCAGGCCCCCTATCAAGGCC AGCCGGTCAGCTGGGGTGGTCACCGTTCCAAAGGAGACCACTGTAGGGGGAACGGCCCCGGGGGTCAGGA GGTTGACTTCCAGCTCCACGGGAGGTGGCTTCAGGGCCACTGCGGTGAGGGCTGCTGTGTGGCTGGGCTC TGCCTTAGTATCCACCTCTGGTTGGGCCTTGCCGGGCACTCTGGCTTCCGGAGGCTCTGGAGGCCCAAGT GCCTGAGAAGGTTCCAGAAACAGCTGCCTCTCCAGGGCCTCCACATGCAGCATGGCTGACATGAGGTTGC CTGCCTTGGCCTGGGCTGTCCTCCTTGGCCGCTGCCTGCTCACTGGGGGCTGAAGCACAGTCGCCTCCTC CCTGGGCTTCAGAAGCAGCTCTGAGGCCACCTGGGGGTCCCCTAGGCCACAGTCCCGAGCTGCCACCAAC AGCAGGTTGTAGCTGTCCCGGCTCGGCTGTAGCCCTAGACTCAGCATCAGCCGCCACACCTGCCACGGAA GAGACAAACGTCAGCTGGGTGCTCCCATTCAGCAGCTGGGCGCAGGGCTCACGCCTGGAATCCCAGCACC TTGGGAGTTTTAGATGGGAGGATCCCTTGAGATCAGGAGTTCGAGACAAGACCGGGCAACATAGCAAGAC CCTGCCTCAAAAAAATACGCCAGGCGCGGTGGCTCACGCCTGTAATCCCAACACTTTAGGAAGCTAAGGC AGGCAGACTCCTTGAGATCAGGAGTTCGAGACCAGCCCGACCAACATGGTGAAACCCCATCTCTACTAAA AATACAAAAATTATTGGGTGTGGTATTGCATGCCCACAGTCCCAGCTACTCAGGAGGCTGAAGTGGGAGA ATCACTTGAACCCAGAGATCGAGGTTGCAGTGAGCCGTGATCATGCCACAGCACTCCAGCCTGGGTGACA GAGCGAGAGTGTCTCAAAACAACTGTAAAACATTTAAAAAATTGGCCGGGCCTCTCCCTCTCCCTCTCCC TCTCCCCCTCCCTCTCCCCACGGTCTCCCTCTCCCTCTTTCCACAGTCTCCCTCTGATGCCCAGCTGAAG CTGGACTGTAACTGCTGCCATCTCGGCTCACTGCAACCTCCCTGCCTGATTCTCCTGCCTCAGCCTGCCG AGTACCTGCGATTGCAGGCGCGCGCCACCACGCCTGACTGGTTTTTGTATTATTTTGGTGGAGACGGGGT TTCGCTGTGTTGGCCAGGCTGGTCTCCAGCTCCTAACCGCGAGTGATCCACCAACCTCGGCCTCCCGAGG TGCCGGGATTGCAGACGGAGTCTCGTTCACTCAGTGCTCAATGGTGCCCAGGCTGGAGTGCAGTGGCGTG ATCTCGGCTCGCTACAACCTCCACCTCCCAGCCGCCTGCCTTGGCCTCCCAAAGTGCCAAGACGGCCGCC ACCCCGTCTGGGAAGTGAGGAGCGTCTCTGCCTGGCCGCCCATCGTCTGGGATGTGAGGAGCCCCTCTGC CTGGCTGCCCAGTCTGGAAAGTGAGGAGCGTCTCTGCCCAGCCGCCATCCCATCTAGGAAGTGAGGAGCG CCTCTTCCCGGCCGCCATCCCATCTAGGAAGTGAGGAGCATCTCTGCCCGGCCGCCCATCGTCTGAGATG TGGGGAGCGCCTCTGCCCCGCCGCCCCGTCTGGGATGTGAGGAGCGCCTCTGCCCGGCCGCGACCCCGTC TGGGAGGTGAGGAGCGTCTCTGCCCGGCCGCCCCGTCTGAGAAATGAGGAGACCCTCTGCCTGGCAACCG CCCCATCTGAGAAGTGAGGAGCCCCTCCGCCTGGCAGCCGCCCCGTCTGAGAAGTGAGGAGCCCCTCCGC CCGGGAGTCACCCTGTCTGGGAAGTGAGGAGCGTCTCCGCCCGGCAGCCACCCCGTCCGGGAGGGAGGTG GGGGTCAGCCCCCGCCAGGCCAGCCGCCCCGTCCGGGAGGGAGGTGGGGGTCAGCCCCCCACCCGGCCAG CCGCCCCGTCCGGGAGGGAGGTGGGGGTGGTCAGCCCCCTGCCCGGCCAGCCGCCCCGTCCGGGAGGTGA GGGGCACCTCTGCCCGGCTGCCCCTACTGGGAAGTGAGGAGCCCCTCTGCCCGGCTAGCACCCCGTCCAG GAGGGAGGTGGGGGGGTCAGCCCCCCGCCCGGCCAGCCGCCCCGTCCGGGAGGGAGGTGGGGGGTCAGCC CCCCGCCCGGCCAGCCGCCCCGTCCGGGAGGTGAGGGGCGCCTCTGCCCGGCCGCCCCTACTGGGAAGTG AGGAGCCCCTCTGCCAGGCCAGCCGCCCCGTCCGGGAGGGAGGTGGGGGGGGTCAGCCCCCCGCCCAGCC AGCCGCCCCGTCCGGGAGGTGAGCGGCGCCTCTGCCCGGCCGCCCCTGCTGGGAAGTGAGGAGCCCCTCT GCCCGGCCACCACCCCGTCTGGGAGGTGTGCCCAGCGGCTCATTGAGAACGGGCCATGATGACAGTGGCG GTTTTGTGGAATAGAAAGGGGGGAAAGGTGGGGAAAAGATTGAGAAATCGGATGGTTGCCGTGTCTGTGT AGAAAGAAGTAGACATGGGAGACTTTTCATTTTGTTCTGTACTAAGAAAAATTCTTCTGCCTTGGGATCC TGTTGATCTGTGACCTTACCCCCAACCCTGTGCTCTCTGAAACATGTGCTGTGTCCACTCAGGGTTAAAT GGATTAAGGGTGGTGCAAGATGTGCTTTGTTAAACAGATGCTTGAAGGCAGCATGCTCCTTAAGAGTCAT CACCACTCCCTAATCTCAAGTACCCAGGGGCACAAACACTGCAGAAGGCCGCAGGGTCGTCTGCCTAGGA AAACCAGAGACCTTTGTTCACTTGTTTATCTGCTGACCTTCCCTCCACTATTGTCCTATGACCCTGCCAA ATCTCCCTCTGCAAGAAACACCCAAGAATGATCAATAAAAAAAAAAAAAAAATTGGCCGGGCGCGGTGGC TCACGCCTGTAATACTAGCATTTTGGGAGAGCAAGGCGCCGGATCACGAGGTAGGAGAGCGAGACCATCC AGGCTAACATGGTGAAACTCTGTCTCTACTAAAAATACAAAAACAAAATTAGCTGGGCATGGTGGTGGGC GCCTATAGTCCCAGCTACTCAGGAGGCTGAGACGGGAGAATGGTGTGAACCCAGGAGGTGGAGCTTGCAG TGAGCCGAGATCATGCCACTGTACTCCAGCCTGGGCGACAGAGCAAGACTCCATCTCAAAAAAAAAAAAA AAAAATTAAAAACATTAAAAAGAAAAAGAAAAAAAAAGCTCTCCAGCGGGGAGCTGTGGTTCACACCTGT AATCCCAGCACTTTGGGAGGCCCAGGCGGGCAGATCACATGAGGCCAGGAGTTCAAGACCAGCCTGGTCA ACATGGTGAAACCCCGTCTCTACTAAAAATACAAAATTAGCTGGGCGTGGTGGCGGGCGCCTGTAATCTC AACTACTCGGGAGGCTGAGGCAGGAGTATCGCGTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAGATC GCTCACTCCAGCCTGGGCAAGAGTGAGACTCCATCTCAAAATAAATGAATGAATGAATGAATGAATGACT CCTGGGTTGCACCTGCAGAAGTGCTAATGGGGGTGTGGGAGGGTTGAACAAGTCCCCAGGTGAGTGTGTG GCAGGCTTTCAAGGAAGGTGAGTTTAGAGGGGCTAAGCCGACTGCAAAGCAGGGTGGGAGGGGCGGGGGC CACCCTCATTCCATCTCCAAGGGCCTCCTCTCCCTGCCATCTGTAGAATGGAGGCTACAGTGGCTGCTGG GTTTTCAGAAAAGAGCACAGATATAAAAATACTCAGCACAGGGCCATCGTGGGTGCTCACAGGATCACCA TTTTGCACCTCTGGCACCGGGGAAGCAGGGCAGGAAGGTGGGGTGGGAGGGACATACCTGGAGGGCGTAC CGGAAGCCTGTCTTCTTGTCTTGGATGCAGCCCATGAGCAGGAAACTGAAGGTCTCCTCTGTGACCACGT GCCCTTTGTGGATGATTTCCTGGGGGAGGGAACAAAGACGTCCCACTGAGAACCTGCAGCAGGCTGGGCA TGGTAGCTCATGTCTGTAATCCTGGCACATTGGGAGGCCAAGGCAGGATGATCACTTGAGGCCAGGAGTT TTGTGACCAGCCTGGGCAACATAGCAAGACCCTGTCTCTAGGAAGCCTTCCCTCGAGTGCACAAAGCAGG ACACAGCACACGGTGGCATACACAATCTCCAGTAAGGTCTCATCAGATCCATCTGTGAACCCCAGGGCCC AATACCGGCTGTCTCATCCTCCCTAAGCCTTCCTACATGAGGAGAGACAGACACCACAGCCCTGCCCCTG ACACGTGGGACCAGCCCCATGAAGGGGAGCAGGACGGCAGGGGAAGCCCCACCTTGAACACATCGAGGCA CATCCTAAGGTCTGCGCACTTGGCAGCCATCTTCAGCAGCGCGTGGTATGTTTTCAAGTTGAGCTCGAAG TTTTTGGCCTGCAGCTGCTGCCGGAGCTTCAGGGCGCTCTGTAGAGCTGAGTCCTTCCAGGGGGACTCGG CACAGACGTTGAACAGGGCCGTGTAGGTGGCGTCCGAGGGCTCCAGGTCCCGCTTTTTCATCTGTGGAGA TGGAAGAAGCTCAGTGGTGGCCCGGGATCAGCTGGACGCACAGGTGAGCAGCTGCCCCAGAGGAGGCTGA GGCTGGAGAGGACCCCGTCACTGCATCCACCAGGCCAGGCTCTAAGGCAGAGCCATGGACACATCAGAGC CCAGCTCTCCAAACTCAGACACATTGAAGGGACTCTGAAAACTGAGCCCACACACCCAGATGTACCAAAC AGGAGCTACCCTGCCCTGGGCACTTGTCAGCCCACTCCAGGCCTGGGGTCCTGGCAGCAAGGTCTTCCTG ATGTCCCCACCTTCAGGCCACCCCGTCAAGGGGAAAGGGCCTGTAGTGCTTACTACCTGAGGGCTCCCGG GCAAGTTAGCACTTCCTCGCGAAAATGGGGATGACACCATCACCTTGAAACCAGGCATGCCTGTGGCAAG TGGGAGGATGCACTGCAGTGCTCTAGGGGTGGCCCCGAACCAGTGGCACAATGCACATTCTCAGGGCCAC CTGATCTGGTAAGTCACAAGCCCCGGAGCTGAGTGACACTCAGCTTACTGAGCCTCCAGGGGACTAAGGC ATGTGCTCAGGGTTGACAATCCGTGGTGAAGCCTAGCACTCGCTATTGGCCAGAGAAGGGCCATCTGGAC AAAGGCAGGCGATAGGCAGCTCAGCGGCAGAAAGAACCCTGCTAGAGTCAGGCCATCTGGGGCTCAAATC CCAGCCCCGGTGCATTCAAGTGGGGTGGCCTTGGACATGTCCCTTCATCTCTCTGTGCCTCAGTTTCCTT ATCTGAAAAGCAAAGAGGGCAGTCCCTACTTTGAGGATGATGTAAGCTCTGCGTGTATTGCTGCACTCCA CAGAGGTGCACTGACAACTCACTACCTGCCCAGCAGCAGCCCTGGGGTTAGCAGAGCGGGCACAGCCTAT CCTTACTGAGCTTACACTCAAGAAGGGAGACACACTGCAGGTGAGTCACCAAAAACAGTAACAAGGCTGG GTGCCGTGGCTCACGCCTATAATCCCAGCACTTTGGAAGGCTGAGGTGGGCAGATCACCTGAGGTCAGGA GTTCGAGACCAGCCTGGCCAACATGGCGAAACCCCGTCTTCTACAAAAAACACAAAAAAATTAGCCAGGC ATGGTGGTGGGCGCCTTTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAACCTGGGAGG TGGAGGTTGCATTGAGCCGAGGTCGCACCACTGCACTCCAGCCAGGGTGACAGAGCCAAAACTCCCGTCT CAAAAAAATAAATAAATAAAATAAAATAAATAAAAACAATAAAAATATTAGTTTTTTTTCTTTTTTTTTG AGATGGAGTCTTACTCTGTCGCCAAAAAAAAGGACTGTAGGAAAAAGAAAGAGAGATCAGACTGTTACTG TGTCTATGTAGAAAGAAGTAGACACAAGAAACTCCATTTTGTTCTGTACTAAGAGAAATTCTTCTGCCTT GAGATGCTGTTAAACTGTAACCCTAGCCCCAACCCTGTGCTTGCAGAGACATGAGCTGTGTTGACTCAAG GTTTAATGGATTTAGGGCTGTGCAGGGTGTGCTTTGTTAAAAAAGTGCTTGAAGGCAGTAGGCTTGATAA AAGTCATCGCCATTCTCCAATCTCGAGTACCCAGGGACACAATACACTGCGGAAGGCCGCAGGGACCTCT GCCCAGGAAAGCCAGGTATTGTCCAAGGTTTCTCCCCATGTGATAGCCTGAGATATGGCCTCGTGGGAAG GGAAAGACCTGACTGTCTCCCAGCCTGTCCCCCAGCCCGACGCCCATAAAGGGTCTGTGCTGAGGAGGGT TAGTGAAAGAGGAAGGCCTCTTTGCAGTTGAGATAAGAGGAAGGCATCTGTCTCCTGCTCGTCCCTGGGA ATGGAATGTCTTGGGTGTAAAACCAGATTGTGTGTTCTATTTACTGAGATAGGCGAAAACCGCCTCAGGG CTGGAGGTGGGACCTGCTGGAGACAATACTGCTCTTTGATGNNNNNNNNNNNNNNNNNNNNAAATGGGGA TGACACCATCACCTTGAAACCAGGCATGCCTGTGGCAAGTGGTAGGATGCACTGCAGTGCTCTAGGGGTG GCCCCGAACCAGTGGCACAATGCACATTCTCAGGGCCACCTGATCTGGTAAGTCACAAGCCCCGGAGCTG AGTGACACTCAGCTTACTGAGCCTCCAGGGGACTAAGGCATGTGCTCAGGGTTGACAATCCGTGGTGAAG CCTAGCACTCGCTATTGGCCAGAGAAGGGCCATCTGGACAAAGGCAGGCGATAGGCAGCTCAGCGGCAGA AAGAACCCTGCTAGAGTCAGGCCATCTGGGGCTCAAATCCCAGCCCCGGTGCATTCAAGTGGGGTGGCCT TGGACATGTCCCTTCATCTCTCTGTGCCTCAGTTTCCTTATCTGAAAAGCAAAGAGGGCAGTCCCTACTT TGAGGATGATGTAAGCTCTGCGTGTATTGCTGCACTCCACAGAGGTGCACTGACAACTCACTACCTGCCC AGCAGCAGCCCTGGGGTTAGCAGAGCGGGCACAGCCTATCCTTACTGAGCTTACACTCAAGAAGGGAGAC ACACTGCAGGTGAGTCACCAAAAACAGTAACAAGGCTGGGTGCCGTGGCTCACGCCTATAATCCCAGCAC TTTGGAAGGCTGAGGTGGGCAGATCACCTGAGGTCAGGAGTTCGAGCCCAGCGTGGCCCACATGGCGAAA CTCTGTCTACTAAAAATACAAACAAAATGAGCCAGGCATGGTGGCATGTGCGCCTGTAGTCCCAGCTACT CGGGAGGCTGAGGTATGAGAACTGCTCGAGCCCAGGAGGCGGAGGTTCCAGTGAGCCGAGGTCGCACCAC TGCACTCCAGCCTGGGCGACAGAGCGAGACTCTGTCTCCAAAAAAAAAGGACTGTAGGAAAAAGAAAGAG AGATCAGACTGTTACTGTGTCTATGTAGAAAGAAGTAGACACAAGAAACTCCATTTTGTTCTGTACTAAG AGAAATTCTTCTGCCTTGAGATGCTGTTAAACTGTAACCCTAGCCCCAACCCTGTGCTTGCAGAGACATG AGCTGTGTTGACTCAAGGTTTAATGGATTTAGGGCTGTGCAGGGTGTGCTTTGTTAAAAAAGTGCTTGAA GGCAGTAGGCTTGATAAAAGTCATCGCCATTCTCCAATCTCGAGTACCCAGGGACACAATACACTGCGGA AGGCCGCAGGGACCTCTGCCCAGGAAAGCCAGGTATTGTCCAAGGTTTCTCCCCATGTGATAGCCTGAGA TATGGCCTCGTGGGAAGGGAAAGACCTGACTGTCTCCCAGCCTGTCCCCCAGCCCGACGCCCATAAAGGG TCTGTGCTGAGGAGGGTTAGTGAAAGAGGAAGGCCTCTTTGCAGTTGAGATAAGAGGAAGGCATCTGTCT CCTGCTCGTCCCTGGGAATGGAATGTCTTGGGTGTAAAACCAGATTGTGTGTTCTATTTACTGAGATAGG CGAAAACCGCCTCAGGGCTGGAGGTGGGACCTGCTGGAGACAATACTGCTCTTTGATGCACGAGATGTTG TGTAGGTGCACATCAAAGCACAGCACCCTTCCTTAAACTTATTTATGACACAGAGACCTTTGCTCACATA TTTTCCTGCTGACCCTCTCCCCATTACCCTATCGTCCTGCCACATCCCCCTCTCCGAGATGGTAGAGATA GTGATCAATAAATACTGAGGGAACTCAAGAGACCAGTGCCTGCGCAGCGCAGGTCCTCCGTATGCGCAGG TCCTCCGTATGCGGGATGCTGAGCGTATGCTGAGCACCGGTCCCAGGGGACCACTGTTCTTTCTCTATAC TTTGTCTCTGTGTCTTATTTCTTTTCTCAGTCTCTCGTCCCACCTGACGAGAAACGCCCACAGGTGTGGA GGGGCTGGCCCCCTTCAGAGGAGCAGATGCGGTGGCTGGGCTGTGACCTGTAATCCCAGCACTTTGGGAG GCTGAGGCGGATGGCCAGGGGTTAGAGACCAGCCTAGACAAAGCAGCACAATCCCACCTCTACAAAAAAC TTAAAAATTAGCCAGATATGGTGCTACACACCTGTGCTCACAGCTACTGGCGAGGCTGAGGCAGGAGGAA CTCTTGAGCCAAGGAGTTGGAGGCTGCAGTGAGCTATGACTGCACTCCAGCCTAGGTGACAGAGTGAGGC TCCCTCTGTTTTTTTTTGAAGGGAAAAGAAAGGGAACATGGGGACCGCCACAGTCCAGGCAGGTGCTAAA GGGCTTTCCATGTCATCTCGTTTATCACCCACAATCACCACCATTTTAAGGAGATGACTCTGAGGCCAGG GAGGTGAAGTCACCTGCCCAGTGTAAAGCACTAGCAAGTGGCACACTTGGGATCCGCCCCCAGCTTTTCA GAGCCTCACCCCGGCTGCCCTGCGCCCACATGCACCTGGTTGTAGAGGTTGAAGGCCTTCTTCAGGTAGC CAACCCGCCCGCAGCCCCCAATCAGCACCGTGTAGTTGCTCTCCATGGGCTGCAATCGCTCCTCCTTCAG CATCTGCCTCTCAAACAGGTCCAGGGCTTCAACCAGCTGCAGGGAAGAGGCAACAGGGCAGGGGCTCAGA ATGGCCCCAGAGACCCTCAGCAGAGAGAGGGAGGTTGAAGCTGAGGGACCCTCCTAAAATGGTGGAGAAC GGCGGCCCTGGGTGACTGCAAACCCCCTGGGCCCCCAGCTGTTTCTGCCCCATCCCTCACCTCACACCAT TTCCCCGGTGAAGCCTCAGTTCATTCCTTTATTCCTTCATTATTTTCATCTGTTCATTCAAACAATAATT ACTGAGTGCTTCAATGAGCCAGACAAGGTCTATCCACTGCATATACGGCTGGGAACAAGAGAGAGCTCCT TCTTGGAGCTCATGTTTTGGTGTGGAAAGTCAGACATGAGGCCAGGCGCAGTGGCTCACGCCTGTAATCC CAGCACTTTGGGAGGCTGAGGCGGGCAGATCACAAGGTCAGGAATTCGAGACCAGCCTGGCCAACATGGT GAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGCATGGTGGCACACACCTGTAATCCCAGCTAC TTGGAAGGCTAAGGCAGAAGAATTGCTTGAACCCGGGAGGCAGAGGCTACGGTGAGCCAAGATTTCACCA CTGCACTCCAGCCTGGGTGACGGTACAAGACTCCATCTCAAAAAAAAAAAAAAAACAAAAAAACAAACCA GACAGACATGAAATGGGATAATTAAGAAAGACACACAGTATATCAAGTGATGACAGGTGGTACGAACAAA ACTAAAGCAGCGGCCAGGTGTAATGGCTCATGTTTGTAGTCCCAGCATTTCGGGAGACCAAGGCGGGCGG ATCACCTGAGGTCTGGAGTTTGAGACCAGCCTGGCCATCATGGAGAAACCCCATCTCTACTAAAAATACA AAAATTGGCCAGGTGTGGTGGCACATGCCTGTAATCCCAGCTACTCAGGATGTTGAGATGGGAGAATTGC TTGAATTCCCCAGGAGGCGGAGGCTGCAGTGAGCCAAGATCGTGCCACTGCACTCCAGCCTGGGCGACAG AGTGAGATCCTGTTATAAAAAAAACCTAAAGCAGGGAGTGCTAGAAGGGTGTGGAGGATTCACTTTTCTT TTTCCTTTTTTTTTTTTTGAGACAGACTCTTGTTCTGTGACTCAGGCTGGAGTCTAGTGGTAAGATCAGC TCAGTGCAACCTCCGTCTCTACAAAAAAAAAAAAATACAATAAAATAAAACTGCGGTGGAGGATGGCAAA GGGTTGCGATACTTACAGCCATAAGGCTGGCAGTTCAACAGGGTAGGCCCAAAACTTAGAGGCTGGGAAA GTCAGGTGACCCCTTGCAAAACACTGAGCCACCAGGGGAAAGAAGCCAGGAGCCACAGAACCCAAAGTGC CCCTTACCTTCCCTTCCTTGATCAGGTGTTTGCACTGCAAGAAGTACCAGTACGGGGTGTTTCTCCGGCC TCGCCATAATTTGGGCTCCGGTTCCATTTGCTCATCTCTCCGTTCCCCAAACCGCAGGTTATGGAACTGG GCTGCGGATTTGCGGAATAGTCTCCGGGAGGAGTATTTGTCAGAGAGGGTCCCAAAACTCTCCTCCTCCT CCTCGTCTTCTTCCTGCGTGGCCGTGGAGTTGGAGTGGCTCGGGTCAGAGCCCAGGCTGCCCGTGTTTTC CTGACGCTCCTGGCCGAGGGGCAGCTGAGAGGAGGAGCTGCTGAAGGGCGCCCACATTGGCCGCATCAGC CCCTCCCTGCCTCCTGCCCACCTGGCTCTACAGGGGTCCAGGTGTTGCAGGATGAACAGTCCCATGGGGC GGGCCCTGGCGAACAGTCGAGCGAGTCTCACGAAGTCCATTTCTGGCTTTCCTGTCCCTCCAGGGCCTGG AATATATCAGGAAAAATAAGAGAGTCAACTCAGTTCCTAGTAACAAAAGGCAGAAAGAAAGAAGTGGTTA CAAGTATGGGCCCAGGCCAGGCGCGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGTCGAGGCTGG CATATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGACAACATGGTGAAACCCCGTCTCTATGAAAAAT ACAAAAATTAGCCAGGCTTGGTGCTGTGCGCCTGTAATCCCAGCTACTCAGGAGGCTGAGGCAGGAGAAT CACCTGAACCTGGGAGATGGAGCTTGCAGTGAGCTGAGATTGCGCCACCATACTCCAACCTGGGTGACAG AGTGAGACTCTGTCTCGAAAAAAAAAAGAAAAGAAAGAAAGAAAAAGAACAGGCCAGGGGCGGTGGCTCA TGCCTGCACTCCCAGCACTTTGGGAGGCCGAGGCGGGCAGATCAGTTCAAGACCAGCCTGGCGACACCCC ATCTCTACTAAAAATACAAAAAACATGAGCTGGGTGTGGTGCTACACGTCTAATCCCAGCTACTGAGGAG ACTGAGGTAGGAGAATCGCTTGAACCCAGGAGACGGAGCTTGCAGTGAGCTGAGATCGCGCCACTGCACT CCAGCCTGAGTGACAGTGAGACTCTGTCTCAGAAAAAAAAAAAAATAATAATAATAAATAAAATAAGAAG TATGGGCCCAGAGGTTAGAAAGCCTAGGTTCAGGTCAGACATAGCTCACTGCAGCCCCACCCTCCTGTGC TCAAGCGATCCTCCCATTTCAGCCTCCCGAGTAGCTGGAACTACAGGTGTGAGCCACTATGCCTGGCTAA TTTTATTTTTTGGTAGAGACAGGGTCTTGCTATGTGCCCAGGCTGGTCTTGAACTCCTGGACTCAAGCAA TCCTCTCGCCTCGACCTCCCAAAGCACTGGGATTACAGCAATGAGCCACCATGCCCAGCCTAAAGTATTT TAGAGTCTGGTTATATTAGAAGGTTGCGATTGGAAAAGTCTTACAAGTGATGGCAGATTGAACAGATTAA AAACTAGGCCCAGTAGGTCAGGAGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGG GTGGATCCCCTCAGGTCAGGAGTTCGAGACCAGCCTGACGTGGTGAACCCTATCTCTGCCGAAAATATAA AAATTAGCTGGGCGTGACAGCGTGCCCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCACT TGAACGTGGGAGGCAGAGGTTGCAGTGAGCCAAGATCGCGCCATTGCACTCCAGCCTGAGCGACAGAGCA AGACTCCATCTCAAAACAACAACAACAACAACAAACAAAAAACTAGGCCCAGCAAGGGGAGAGACTTGCT GGGGTCCTGCAGCAGTGCAGTGGGTGAGACTGGCCAGCTTGGGCTGGGCTGGCTTTGAACTCAGGTCCAT GTAACGCATTGATGATTGTCCTGACCAGCACCTTTTGTGAAACTAGTGCAGGGGGCCTTTATCATTCTGC ATCTGGCAAGCCCTGCCACTCCTTCTTACAAGCATGGTGTTTATGTAATTTTCTTTTTTGTGGAATGGGG TCTTCATGTTGCCCAGGCTGGAGTGCGATGGCACAGTGGAGTGCAAATGCACAATCACTGCAACCTCCAA CTCCTGGGCTCAAGTGATCCTCCTACCTCAGCCTCTCAATTAACTAGCACTACTGGTGCACACCACCATA CCTGTCTAATTTTTTGTATTTTTTTGTAGAGATGGAGTCTCCCTATGTTGCCAGGCTGGTCTAGAACTCC TGGCCTCAAGCAATCCTCCAGCCTTGGCCTCCCAAAGTGTTGGGATTGCAGGCGTAAGCCTCCATGCCCT GCCTTACGTGCATTAATTACAATACACAGAATTACAAAACAAAAAGCAATGATATTGAAATGGCATCAAA ATGCTTTAAAAACAAATTTCTGCTATACTAGTATAGATGCTTATTTTCAATGCATGAAATAACAAGACCC TATCATAATTTCTTTTCTTTTCTTTTTTTTTTTTTGAGACAGTCTCTCACTCTGTCCTCAGGCGTGACCT CGGCTCACTGCAATTTCCGCCTCCCGGATTCAAGCGATTCTCCTGCCTCAGCCTCCCCTGGAGCTGGGAC TACAGACGCATGCCACCAAGCCCAGCTAATTTTTGTATTTTTAGTAGAGACAGGGTTTCACCATGTTGGC CAGGATGGTCTGGATCTTTTGACCTTGTGATCCGCCCGCCTCGGCCTCCCGAAGTGCTGGGATTACAGCC GTGAGCCACCGCGCCCGGCCGACTCTATCATAATTTCAAAGCAGTAATAAATGTAAGTGACAGCTTAAGA TACCACCTTGTAGTTGCTGCACAAACTGGGTTTTTTTGCGTCTCACTCTGTCACCAGGCTGGAGTGGGGT GGTACAATCTCGGCTCAATGCAACCTCTGCCTCCCGGGTTTAAGCCATGCTCATGCCTCAGCCTCCCAAG TAGCTGGGATTACAGGAACGCGCCACTACTCCAGGCCAATTTTTATATTTTCAGTAGGGACGGGGTTTTG CCATCCTGTCCAGGCTGGTCTCGAACTCCTGACCTCACGTGATCCTCTCGCCTCAGACTCCCAAAGTGCT GAGGTTACAGGTGTGAGCCACCATGCCTGGCCCTGCACAAACTGTTATCTGGAACTATCATTTACACTCA CCCTCACAGTTTCAGGCACTGCTAAGGCTGCTCCAGCAATCAGGAAAGGAAATGCTATACTTCACATAGA GAAAAATAGGGGTGCATTTTGTTCCTACACAAGCTCATTACCCCACCCTGGTTAAAAAAAACCCTGAATT AGGCTGTATAGTGGCTCATGCCTGCAATTCCAGCACTTTGGGAGGCCCAGGCAGGAGGATCGCTTTGGGC TTGGGGCCAGGAGTTCGAGACCAGCCTGGGCAACATAGCAAGACCCGCCACCCGCCCCCCCAACCCCGCC GTCTCTACAAAAATTAAAAATAAAATTTTAAAAATTAGCCAAGTGTGGTGGTGCAGCGTCTGTAGTTCCA GCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGTCTGAGAAGTTGACTGCAGTGAGCTATGATCGTA CCCCTGTACTCCAGCCTGGGCCACAGAGCGAGAAAAAACCCTGAATTAGAGACACGCTGCGGCCGTCCTT CCTCGGAGAGACCCGCCCCTCCTCAGAGGCCCACCCGGACCCTCAGGCCAACGCCGACCCCTCTCCACAC GGACTTCTGCAGCCTCAGTTTCCCTCCTCAGACGCCCCCGGCTCCAATCCCGGCTCGGGCACTTCCTTCC CCTCTGGCGTGTCTTGGCCCGGGTCCCGGGGCTCCTGCGAGGATCACACAGGAACTCACTTGAAGTGTCC GGCGCAGTGCACTCCGACGGGGAGCCCTGCCCGGTCCCCGCGGCGAACCAGTCTCTTCCTCGGGTCCCCC TCTCCCCAAGCGCGCAGGCGCAATCCGCGTCGCCGACTCACGCGGCTGGTTTCAGGTTTCTGGCGA >gi|38492358|ref|NP_056360.2| pentatricopeptide repeat-containing protein 1 [Homo sapiens] MDFVRLARLFARARPMGLFILQHLDPCRARWAGGREGLMRPMWAPFSSSSSQLPLGQERQENTGSLGSDP SHSNSTATQEEDEEEEESFGTLSDKYSSRRLFRKSAAQFHNLRFGERRDEQMEPEPKLWRGRRNTPYWYF LQCKHLIKEGKLVEALDLFERQMLKEERLQPMESNYTVLIGGCGRVGYLKKAFNLYNQMKKRDLEPSDAT YTALFNVCAESPWKDSALQSALKLRQQLQAKNFELNLKTYHALLKMAAKCADLRMCLDVFKEIIHKGHVV TEETFSFLLMGCIQDKKTGFRYALQVWRLMLSLGLQPSRDSYNLLLVAARDCGLGDPQVASELLLKPREE ATVLQPPVSRQRPRRTAQAKAGNLMSAMLHVEALERQLFLEPSQALGPPEPPEARVPGKAQPEVDTKAEP SHTAALTAVALKPPPVELEVNLLTPGAVPPTVVSFGTVTTPADRLALIGGLEGFLSKMAEHRQQPDIRTL TLLAEVVESGSPAESLLLALLDEHQVEADLTFFNTLVRKKSKLGDLEGAKALLPVLAKRGLVPNLQTFCN LAIGCHRPKDGLQLLTDMKKSQVTPNTHIYSALINAAIRKLNYTYLISILKDMKQNRVPVNEVVIRQLEF AAQYPPTFDRYQGKNTYLEKIDGFRAYYKQWLTVMPAEETPHPWQKFRTKPQGDQDTGKEADDGCALGGR Vaximmutor RD1 Mycobacterium tuberculosis H37Rv 885777 15610750 Rv3614c BX842583 NP_218131 83332 4054141 4054695 - hypothetical protein 3.73 18643.7 184 Rv3614c, (MTCY07H7B.08), len: 184 aa. Conserved hypothetical protein, equivalent to Q49730|ML0407|B1620_C3_264|MLCL383.03 HYPOTHETICAL 24.2 KDA PROTEIN from Mycobacterium leprae (216 aa) FASTA scores: opt: 899, E(): 1.7e-51, (71.3% identity in 188 aa overlap); and similar to two hypothetical proteins from Mycobacterium leprae: Q9CDD6|ML0056 (169 aa), FASTA scores: opt: 285, E(): 1.2e-11, (38.35% identity in 172 aa overlap); and O33090|MLCB628.19c (338 aa), FASTA scores: opt: 289, E(): 1.2e-11, (38.95% identity in 172 aa overlap). Also highly similar to O69732|Rv3867|MTV027.02 HYPOTHETICAL 19.9 KDA PROTEIN from Mycobacterium tuberculosis (183 aa), FASTA scores: opt: 563, E(): 1e-29, (54.9% identity in 173 aa overlap). >gi|57116681:4054141-4054695 Mycobacterium tuberculosis H37Rv, complete genome CTCACCATGGATCGCTCTCGTCGTCTGGTGCTGGACAATCGTCGCTGTAGCGCGTCGCGAACACCTCAGC TTCTGCTGCCGCGGCTTCTTCCGGCGATGGTAACCCCCAGGTTTCGCCCACGGTCTTACGTAGCAGTGCG ACGCGGTGTTCATCTGCATCGACCTGTTGACTCATCCTGTCAAGGATGAAGGCGTACTGGGCCGACTGCG CCTTCTGCCGCGCCAGGTCGGCAATCACCAGGATCTCAGAAGCGAGCTGCGACTCACTCATCCAGGCCAC CCTGGCCGACAGCTCGACATGGTCAATCCGGCCGTCCATCAGCGTCGATACCGACACCGTGCGTGGGGGA TTCGTCACGGTAAAAAGCGCGATCTCTTGTTCGGTGTCCGTCTCCGCCTGACCGTGGGCATTGTCCAGGT CGGGTCCGGTGTCCGGGGTCGCCGCCGACCCGACGCCAATAATCGGATCCGCAGTCCAGCCCTCCGCGCC GTCGGCACCCCAGAGATCCACGGCGTCGAAATCGTTGCTGTCAAAGTCATTTCCGGGCAAGTCCA >gi|15610750|ref|NP_218131.1| hypothetical protein Rv3614c [Mycobacterium tuberculosis H37Rv] MDLPGNDFDSNDFDAVDLWGADGAEGWTADPIIGVGSAATPDTGPDLDNAHGQAETDTEQEIALFTVTNP PRTVSVSTLMDGRIDHVELSARVAWMSESQLASEILVIADLARQKAQSAQYAFILDRMSQQVDADEHRVA LLRKTVGETWGLPSPEEAAAAEAEVFATRYSDDCPAPDDESDPW Virmugen RD1 and panCD mutants in Mycobacterium tuberculosis were attenuated and provided protection in CD4-deficient mice against an aerosol challenge with virulent M. tuberculosis [Ref1961:Sambandamurthy et al., 2006]. relG Mycobacterium tuberculosis H37Rv 887450 15610003 Rv2866 AL123456 NP_217382 83332 3177821 3178084 + toxin RelG 11.51 9542.41 87 Also known as relE2RelG, toxin, part of toxin-antitoxin (TA) operon with Rv2865 >NC_000962.3:3177821-3178084 Mycobacterium tuberculosis H37Rv, complete genome GGTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGCATCCTC GCGGCAGTGGTCGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGC GCGAGTTGGCCGGCACGTTCAGCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCA CACAACGGTAGTGATCCTGCGCGTCGATCACCGCGCGGACATCTACCGCCGATA >NP_217382.1 toxin RelG [Mycobacterium tuberculosis H37Rv] MPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEH TTVVILRVDHRADIYRR Protective antigen ripA Mycobacterium tuberculosis H37Rv 886541 15608615 Rv1477 AL123456 NP_215993 83332 1666989 1668407 + peptidoglycan endopeptidase RipA 9.34 46335.23 472 peptidoglycan hydrolase >NC_000962.3:1666989-1668407 Mycobacterium tuberculosis H37Rv, complete genome AATGAGACGGAATCGCCGTGGCTCGCCAGCGCGACCGGCCGCACGGTTTGTCCGTCCGGCAATTCCGTCG GCTTTGAGTGTGGCCCTGCTGGTATGCACACCGGGGCTGGCTACCGCCGATCCACAGACGGACACCATCG CCGCGCTGATTGCCGACGTCGCCAAGGCCAACCAGCGCCTGCAAGACCTGAGCGACGAGGTTCAGGCCGA ACAGGAAAGCGTTAACAAGGCGATGGTCGACGTGGAAACCGCTCGGGACAACGCTGCCGCGGCCGAAGAC GACCTGGAGGTCAGCCAGCGCGCGGTTAAGGACGCCAACGCGGCGATCGCCGCGGCTCAGCACCGGTTCG ACACCTTCGCGGCGGCCACCTACATGAACGGTCCCTCGGTCAGCTACCTCAGCGCGAGCAGCCCCGACGA GATCATTGCCACTGTGACCGCCGCCAAGACCCTTAGCGCCAGTTCCCAAGCGGTGATGGCCAACCTGCAG CGGGCCCGGACCGAGCGGGTGAACACGGAGTCGGCGGCGCGGCTAGCCAAGCAGAAGGCTGATAAGGCCG CCGCCGACGCAAAGGCCAGCCAGGATGCCGCGGTGGCGGCGCTCACCGAGACCCGGCGGAAGTTCGATGA ACAGCGCGAGGAGGTCCAACGCCTGGCCGCCGAGCGCGATGCGGCTCAAGCCCGACTGCAGGCGGCCAGG TTGGTTGCCTGGTCCTCGGAGGGTGGTCAGGGTGCGCCGCCGTTCCGGATGTGGGATCCCGGATCGGGCC CTGCCGGTGGGCGTGCATGGGATGGCTTGTGGGACCCCACGCTGCCCATGATCCCCAGCGCCAACATCCC CGGCGACCCGATCGCGGTAGTGAACCAGGTGTTGGGGATCTCGGCAACGTCAGCGCAGGTCACCGCCAAT ATGGGGCGCAAGTTCCTGGAGCAGCTGGGCATCTTGCAGCCCACCGATACCGGCATCACCAACGCTCCGG CGGGCTCGGCCCAGGGCCGGATTCCGCGAGTTTATGGGCGCCAGGCTTCTGAATACGTGATCCGCCGCGG CATGTCACAGATCGGGGTGCCCTATTCCTGGGGCGGCGGCAATGCCGCGGGCCCGAGCAAGGGCATCGAC TCCGGGGCCGGCACCGTCGGCTTCGACTGCTCAGGCCTGGTGTTGTACTCGTTTGCTGGGGTGGGCATCA AGCTGCCGCACTACTCGGGTTCGCAGTACAACCTGGGCCGCAAGATCCCGTCCTCGCAGATGCGCCGCGG CGACGTCATCTTCTACGGCCCGAACGGTAGCCAGCACGTGACGATCTACCTCGGCAACGGCCAGATGCTC GAGGCGCCCGACGTCGGTTTGAAGGTGCGGGTTGCGCCCGTGCGCACGGCTGGCATGACCCCGTATGTGG TCCGATACATCGAGTACTA >NP_215993.1 peptidoglycan endopeptidase RipA [Mycobacterium tuberculosis H37Rv] MRRNRRGSPARPAARFVRPAIPSALSVALLVCTPGLATADPQTDTIAALIADVAKANQRLQDLSDEVQAE QESVNKAMVDVETARDNAAAAEDDLEVSQRAVKDANAAIAAAQHRFDTFAAATYMNGPSVSYLSASSPDE IIATVTAAKTLSASSQAVMANLQRARTERVNTESAARLAKQKADKAAADAKASQDAAVAALTETRRKFDE QREEVQRLAAERDAAQARLQAARLVAWSSEGGQGAPPFRMWDPGSGPAGGRAWDGLWDPTLPMIPSANIP GDPIAVVNQVLGISATSAQVTANMGRKFLEQLGILQPTDTGITNAPAGSAQGRIPRVYGRQASEYVIRRG MSQIGVPYSWGGGNAAGPSKGIDSGAGTVGFDCSGLVLYSFAGVGIKLPHYSGSQYNLGRKIPSSQMRRG DVIFYGPNGSQHVTIYLGNGQMLEAPDVGLKVRVAPVRTAGMTPYVVRYIEY Protective antigen rpfC Mycobacterium tuberculosis H37Rv 885759 15609021 Rv1884c AL123456 NP_216400 83332 2133730 2134260 - resuscitation-promoting factor RpfC 9.94 17450.38 176 >NC_000962.3:2133730-2134260 Mycobacterium tuberculosis H37Rv, complete genome GTCAGCGCGGAATACTTGCCTGAATGCCTGCCCAAATGATCTCGTTGATGATTTGCTTGATGCCCTGCGC GGGTTTCGACCACAGTGCGATCGGAAGGCCAGAGGCGGCGCCGCACGTCGGCCACGCGTCCAATCCCTGT TCGGCGAGAACCCGATTGGCAACTGCGATTTGTTGTTCCCGAGAGGCAGCTGCTGGGTTGCCGACACCGC CGAATGCGGCCCAGGTGGCCGGCTTGAACTGCAGTCCGCCGTATTTGCCGTTTCCGGTGTTGGCCGCCCA GTTGCCCCCGGATTCGCACTGCGCGACGGCGTCCCAGTTCGGGCTGGGACCGGCGTGGGCAACGGCGGTG GAGAGCGACATGGATGCCGTGACGAGTCCTGCGGCCATGGCGGACTTGATGAGCGGCTTGGCGATTCTTG TCATGCTCGACATATCGCCGGAAGTGGCCGAAGCGTTACCGATTAGAGAGAGTGGTGAGATCGGGTGTCT ATTGCACCGCGACCGGCCGTGGTCGGCCGGCAAAGGATGCA >NP_216400.1 resuscitation-promoting factor RpfC [Mycobacterium tuberculosis H37Rv] MHPLPADHGRSRCNRHPISPLSLIGNASATSGDMSSMTRIAKPLIKSAMAAGLVTASMSLSTAVAHAGPS PNWDAVAQCESGGNWAANTGNGKYGGLQFKPATWAAFGGVGNPAAASREQQIAVANRVLAEQGLDAWPTC GAASGLPIALWSKPAQGIKQIINEIIWAGIQASIPR Protective antigen Rv0572c Mycobacterium tuberculosis H37Rv 887696 15607712 Rv0572c AL123456 NP_215086 83332 665041 665382 - hypothetical protein 9.09 11918.46 113 >NC_000962.3:665041-665382 Mycobacterium tuberculosis H37Rv, complete genome TTCATAGGTCATCGGATTGAGGTGATCGACTCGGCGATGAGTGTTCGAAAGATGACTCAGTGGTGTGCCT TCCGTCGGTGAGCTGCACGACATATGTGCGGTCGTCGGCGTCGTACTCAACCGTGCCGCCCGAGACAACC ATCCCAACCAACGCATTGCCGTCCTGGAAGTAGTACGCGTTCTTTTCTCGGCGCATGGAATCCAGGTGGC AACCGGGCACTATGAGGACGCTCCTGCGGGGATCGTCGGTGAAGACCAGAATCCGCGCGCCCCGTTTCTG GGCTAGGGGATGCTTCGTAGGCTTCCGTTGCCGCATGTGCCGCTTGATGGCGTGCTCACCCA >NP_215086.1 hypothetical protein Rv0572c [Mycobacterium tuberculosis H37Rv] MGEHAIKRHMRQRKPTKHPLAQKRGARILVFTDDPRRSVLIVPGCHLDSMRREKNAYYFQDGNALVGMVV SGGTVEYDADDRTYVVQLTDGRHTTESSFEHSSPSRSPQSDDL Protective antigen RV1498A Mycobacterium tuberculosis H37Rv 3205040 57116877 Rv1498A AL123456 YP_177647 83332 1690133 1690345 - hypothetical protein 6.23 6835.19 70 >NC_000962.3:1690133-1690345 Mycobacterium tuberculosis H37Rv, complete genome TTCAGGAATCCTCCAGGCGGAAGCCGACTTTCATAGTCACCTGGAAGTGCGCGACCGCTCCGTCGACCAG GTGGCCTCGAATTGACTGTACTTCGAACCAGTCCAGCGCGCGCATGGTCTGCGCAGCTCGGGCCAGACCG CCCTGGATTGCCGCGTCGACGCCGTCGGGCGAGGTCCCGACGATCTCGATCACTCGGTAGGTGTGATTGC TCA >YP_177647.1 hypothetical protein Rv1498A [Mycobacterium tuberculosis H37Rv] MSNHTYRVIEIVGTSPDGVDAAIQGGLARAAQTMRALDWFEVQSIRGHLVDGAVAHFQVTMKVGFRLEDS Protective antigen Rv1626 Mycobacterium tuberculosis H37Rv 885080 15608764 Rv1626 AL123456 NP_216142 83332 1828179 1828796 + two-component system transcriptional regulator 4.74 21081.76 205 >NC_000962.3:1828179-1828796 Mycobacterium tuberculosis H37Rv, complete genome CATGACCGGCCCCACCACCGACGCCGATGCCGCTGTCCCACGTCGGGTCTTGATCGCGGAAGATGAAGCG CTCATCCGCATGGACCTGGCCGAGATGTTGCGAGAGGAGGGATATGAAATTGTCGGCGAGGCCGGCGACG GCCAGGAAGCCGTCGAGCTGGCCGAGCTGCACAAGCCCGACCTGGTGATCATGGACGTGAAGATGCCGCG CCGGGACGGGATCGACGCCGCATCCGAAATCGCCAGCAAACGTATTGCCCCGATCGTGGTGCTGACCGCG TTCAGCCAGCGTGATCTGGTCGAACGTGCGCGTGATGCCGGGGCGATGGCATACCTGGTAAAGCCTTTCA GCATCAGCGACCTGATTCCAGCGATTGAATTGGCGGTCAGCCGGTTCAGGGAGATCACCGCGTTGGAAGG CGAGGTGGCGACGCTATCTGAACGGTTGGAAACCCGCAAGCTGGTGGAACGAGCAAAAGGCCTGCTGCAG ACCAAACATGGGATGACCGAGCCGGACGCTTTCAAGTGGATTCAACGTGCCGCCATGGATCGGCGCACCA CCATGAAGCGGGTGGCCGAAGTCGTGCTGGAAACCCTCGGAACACCCAAAGACACCTG >NP_216142.1 two-component system transcriptional regulator [Mycobacterium tuberculosis H37Rv] MTGPTTDADAAVPRRVLIAEDEALIRMDLAEMLREEGYEIVGEAGDGQEAVELAELHKPDLVIMDVKMPR RDGIDAASEIASKRIAPIVVLTAFSQRDLVERARDAGAMAYLVKPFSISDLIPAIELAVSRFREITALEG EVATLSERLETRKLVERAKGLLQTKHGMTEPDAFKWIQRAAMDRRTTMKRVAEVVLETLGTPKDT Protective antigen Rv1813c Mycobacterium tuberculosis H37Rv NP_216329 CDD:316353 83332 ? hypothetical protein 10.16 14879.86 224 Domain of unknown function (DUF4189); pfam13827 >NP_216329.1 hypothetical protein Rv1813c [Mycobacterium tuberculosis H37Rv] MITNLRRRTAMAAAGLGAALGLGILLVPTVDAHLANGSMSEVMMSEIAGLPIPPIIHYGAIAYAPSGASG KAWHQRTPARAEQVALEKCGDKTCKVVSRFTRCGAVAYNGSKYQGGTGLTRRAAEDDAVNRLEGGRIVNW ACN Protective antigen ID93/GLA-SE protects against TB and MDR-TB in animals.[Ref5076:Bertholet et al., 2010] Rv2028c Mycobacterium tuberculosis H37Rv 888494 15609165 Rv2028c AL123456 NP_216544 83332 2274568 2275407 - universal stress protein 6.31 25771.85 279 >NC_000962.3:2274568-2275407 Mycobacterium tuberculosis H37Rv, complete genome GTCACAGATACTGCTGACCGACGACCAGCAGGGTGCAGCCGGCCTCCTGCAACACGGCGTTGCCCGGCGC TCCCACAAGTTGCTCCACATGCTCCTGGTCGCTCGCGCTGAGCACCACCATGTGTACCGATCGACCCAGC CCAGCCAGATAATCCAGCAGCTCGCCGTGCACTGCCGCCGATTGCACCCGCACATCGGGATACCGTGGTT GCCAACGGGCAAGCCAGCGGTCCAGGCTGGCACGGACGTCGTCCCCGGTATCGCCCACTCCGGATTGCCG GCAGGTGACCACCCGAACCGGCGAGTCGCGCAGCCGTGCTTCGGCCATCACCGCCCCCAGCAAAACACCG ATATCGGACGACCCGTCCGCCTCGACGACGATCCATGCGGCGTCGCGTCCGATGGGGACCCGGTGGGGTC GCACGATCGCCACTGGGCACTGCGCCGATAACGCCAGGGCCGCTGCGGTAGATCCCACCCGCTCCGGTCG GAAGTGGTGCACGCCGATAGCGCCAACGCACACCAGGGCAGCAGCCGCCGAAGCGCGGATCAACGAGGTG ACCGGCCGCTCCTGGGTGATCTCCACCTCGACCTTGACCGGCCGGTCCGCCGCCTCGACCGCTGTGAACG CGTAGCGCACCGCGTTCTCGGCGGCGGCGAGTTTGCGAGCCGCCGCGCCGTGTGCGGCGTACCCGGGATC GTCGGGTTCGATCGCGTACAGCAGACGCAGCGGGATGTCACGGCTGGCTGCCTCGTCGACCGCCCACAGT GCGGCTTGCACGGCCGGCTTCGAGCCATCAATACCGACGACGATCGATGGGGGTTTGTGTGATTGGTTCA >NP_216544.1 universal stress protein [Mycobacterium tuberculosis H37Rv] MNQSHKPPSIVVGIDGSKPAVQAALWAVDEAASRDIPLRLLYAIEPDDPGYAAHGAAARKLAAAENAVRY AFTAVEAADRPVKVEVEITQERPVTSLIRASAAAALVCVGAIGVHHFRPERVGSTAAALALSAQCPVAIV RPHRVPIGRDAAWIVVEADGSSDIGVLLGAVMAEARLRDSPVRVVTCRQSGVGDTGDDVRASLDRWLARW QPRYPDVRVQSAAVHGELLDYLAGLGRSVHMVVLSASDQEHVEQLVGAPGNAVLQEAGCTLLVVGQQYL Protective antigen Rv2034 Mycobacterium tuberculosis H37Rv 887859 15609171 Rv2034 AL123456 NP_216550 83332 2281293 2281616 + ArsR family HTH-type transcriptional repressor 9.75 11161.82 107 >NC_000962.3:2281293-2281616 Mycobacterium tuberculosis H37Rv, complete genome CGTGTCCACTTACAGATCACCGGATCGCGCTTGGCAGGCGCTGGCGGACGGCACTCGCCGGGCCATCGTG GAGCGGCTGGCGCACGGCCCGCTGGCCGTCGGCGAGTTGGCCCGCGACCTGCCCGTCAGCCGACCCGCGG TGTCACAGCACCTCAAAGTGCTCAAGACCGCCAGGCTGGTGTGCGACCGCCCCGCGGGAACACGCCGCGT CTACCAGCTCGACCCGACAGGCCTTGCGGCATTGCGCACCGACCTCGACCGGTTCTGGACACGCGCCCTG ACTGGCTACGCGCAGCTCATCGACTCCGAAGGAGACGACACATG >NP_216550.1 ArsR family HTH-type transcriptional repressor [Mycobacterium tuberculosis H37Rv] MSTYRSPDRAWQALADGTRRAIVERLAHGPLAVGELARDLPVSRPAVSQHLKVLKTARLVCDRPAGTRRV YQLDPTGLAALRTDLDRFWTRALTGYAQLIDSEGDDT Protective antigen Rv2628 Mycobacterium tuberculosis H37Rv 888566 15609765 Rv2628 AL123456 NP_217144 83332 2955057 2955419 + hypothetical protein 9.21 12136.76 120 >NC_000962.3:2955057-2955419 Mycobacterium tuberculosis H37Rv, complete genome CATGTCCACGCAACGACCGAGGCACTCCGGTATTCGGGCTGTTGGCCCCTACGCATGGGCCGGCCGATGT GGTCGGATAGGCAGGTGGGGGGTGCACCAGGAGGCGATGATGAATCTAGCGATATGGCACCCGCGCAAGG TGCAATCCGCCACCATCTATCAGGTGACCGATCGCTCGCACGACGGGCGCACAGCACGGGTGCCTGGTGA CGAGATCACTAGCACCGTGTCCGGTTGGTTGTCGGAGTTGGGCACCCAAAGCCCGTTGGCCGATGAGCTT GCGCGTGCGGTGCGGATCGGCGACTGGCCCGCTGCGTACGCAATCGGTGAGCACCTGTCCGTTGAGATTG CCGTTGCGGTCTA >NP_217144.1 hypothetical protein Rv2628 [Mycobacterium tuberculosis H37Rv] MSTQRPRHSGIRAVGPYAWAGRCGRIGRWGVHQEAMMNLAIWHPRKVQSATIYQVTDRSHDGRTARVPGD EITSTVSGWLSELGTQSPLADELARAVRIGDWPAAYAIGEHLSVEIAVAV Protective antigen Rv2660c Mycobacterium tuberculosis H37Rv 887222 15609797 Rv2660c CP009480 NP_217176 83332 2980962 2981189 - hypothetical protein 4.63 6788.85 75 Rv2660c, (MTCY441.29c), len: 75 aa (questionable orf). Hypothetical unknown protein. >NC_000962.3:2980962-2981189 Mycobacterium tuberculosis H37Rv, complete genome ACTAGTGAAACTGGTTCAATCCCAGTATCGCGCACCACGATTGACCTGCGGTTTCATCCACAAAATCTGG GCTGCGTGAACTAAATGTGAACTGACTCGGTGCAACCACCGAAAGGTTCCTCTGTTCCGTGCCCACGCCG ACACCGACGGTGACCCCACCAGATGCGCCTGCCGCCCGCTGGCTAGCCTGGCCTGTTGCTGCAAGCGCCT GGTCGACGCCCGCTATCA >NP_217176.1 hypothetical protein Rv2660c [Mycobacterium tuberculosis H37Rv] MIAGVDQALAATGQASQRAAGASGGVTVGVGVGTEQRNLSVVAPSQFTFSSRSPDFVDETAGQSWCAILG LNQFH Protective antigen When combined with AG85B and ESAT-6, Rv2660c provided mice protection against challenge [Ref2006:Aagaard et al., 2011]. Rv3126c Mycobacterium tuberculosis H37Rv 888806 15610263 Rv3126c AL123456 NP_217642 83332 3491807 3492121 - hypothetical protein 8.83 11072.67 104 This region is a possible MT-complex-specific genomic island (See Becq et al., 2007 PMID:17545187). >NC_000962.3:3491807-3492121 Mycobacterium tuberculosis H37Rv, complete genome TTCATGGATTGTCAATGACAGCCCAAACCGCCTTCGGCCACTGGCATTGGTGCACTGCACCGTGCGCCAT TCGTGGCGACAACTGCGAGCGGGAGCGGGACCAAGGATGATGGTCCCGGTCGCGACGGGCGCGATCCCGC TCCGGAGTGGTCAACGCATCAAACGACAAAGCGCTCAGCTCATCGACCGCAGCATCGAGCCGGTCCAGCG CCGCGACCAAACTAGAATTCTCGCGCAGACACCGCTGAAACGACAGTGACGCAAGGGATTTCATTGAGAG GACCAATGACCCTATTTGATCAAACCGGATGACCA >NP_217642.1 hypothetical protein Rv3126c [Mycobacterium tuberculosis H37Rv] MVIRFDQIGSLVLSMKSLASLSFQRCLRENSSLVAALDRLDAAVDELSALSFDALTTPERDRARRDRDHH PWSRSRSQLSPRMAHGAVHQCQWPKAVWAVIDNP Protective antigen SecA2 Mycobacterium tuberculosis H37Rv VO_0010950 885594 15608958 Rv1821 BX842577 NP_216337 1NL3 EMBL: AE000516 EMBL: BX842577 GenomeReviews: AE000516_GR GenomeReviews: AL123456_GR HSSP: O05885 InterPro: IPR000185 InterPro: IPR001650 InterPro: IPR011115 InterPro: IPR011116 InterPro: IPR011130 InterPro: IPR014018 InterPro: IPR014021 KEGG: mtc:MT1869 KEGG: mtu:Rv1821 Pfam: PF01043 Pfam: PF07516 Pfam: PF07517 PIR: F70720 PRINTS: PR00906 PROSITE: PS01312 PROSITE: PS51196 TIGR: MT1869 TubercuList: Rv1821 UniProt: P66785 83332 2066456 2068882 + preprotein translocase subunit SecA 5.11 82502.57 808 SecA2; functions in protein export; can interact with acidic membrane phospholipids and the SecYEG protein complex; binds to preproteins; binds to ATP and undergoes a conformational change to promote membrane insertion of SecA/bound preprotein; ATP hydrolysis appears to drive release of the preprotein from SecA and deinsertion of SecA from the membrane; additional proteins SecD/F/YajC aid SecA recycling; exists in an equilibrium between monomers and dimers; may possibly form higher order oligomers; proteins in this cluster correspond to SecA2; which is non-essential and seems to play a role in secretion of a subset of proteins FUNCTION: Involved in protein export. Interacts with the secY/secE subunits. SecA has a central role in coupling the hydrolysis of ATP to the transfer of pre-secretory proteins across the membrane (By similarity) (UniProt: P66785) SUBUNIT: Part of the prokaryotic protein translocation apparatus which comprise secA, secB, secD, secE, secF, secG and secY (By similarity) (UniProt: P66785) SUBCELLULAR LOCATION: Cell membrane; peripheral membrane protein; cytoplasmic side (By similarity) (UniProt: P66785) SIMILARITY: Belongs to the secA family (UniProt: P66785) >gi|57116681:2066456-2068882 Mycobacterium tuberculosis H37Rv, complete genome CGTGAACGTGCACGGTTGTCCACGAATTGCGGCCTGTCGGTGTACAGACACGCACCCTCGCGGCCGGCCG GCATTCGCGTACCGTTGGTTTGTGCCCAAGACCACCCGCGCTCAACCCGGCCGGCTGAGCAGCCGATTCT GGCGATTGCTCGGCGCCAGCACCGAAAAGAACCGGAGCCGCTCCCTGGCGGATGTAACCGCTTCGGCAGA ATACGACAAGGAAGCTGCCGATCTGTCCGACGAGAAGCTGCGTAAGGCGGCAGGCCTGCTCAACCTCGAC GACCTCGCGGAGTCCGCCGATATCCCGCAGTTTCTCGCGATTGCCCGGGAAGCCGCCGAGCGGAGGACCG GGCTGCGACCATTTGATGTGCAGTTGCTTGGCGCGTTGCGCATGCTCGCCGGAGACGTGATCGAGATGGC CACCGGTGAGGGCAAAACCCTTGCCGGGGCGATCGCGGCCGCCGGTTATGCGCTGGCCGGCCGGCACGTG CACGTCGTGACGATTAACGATTACCTGGCCCGCCGCGATGCGGAGTGGATGGGCCCGCTGCTGGACGCGA TGGGCCTGACGGTCGGCTGGATCACCGCGGACTCGACCCCTGACGAGCGCCGGACCGCATATGACCGTGA TGTCACCTATGCCTCGGTCAACGAGATTGGCTTCGATGTACTGCGCGATCAGTTGGTGACTGATGTCAAT GACCTGGTATCGCCCAATCCAGACGTGGCTCTCATCGACGAAGCCGACTCCGTGCTGGTCGACGAGGCGC TGGTGCCCCTGGTGCTGGCCGGAACCACACATCGTGAGACGCCGCGGCTGGAGATCATCCGGCTGGTCGC TGAGCTTGTTGGCGACAAGGACGCCGACGAGTACTTTGCCACCGATTCCGATAACCGCAATGTCCACTTG ACCGAGCACGGGGCACGCAAAGTCGAGAAAGCGCTCGGTGGCATCGACCTGTACTCCGAGGAGCACGTCG GCACCACACTGACTGAGGTCAATGTCGCGCTGCACGCGCATGTGCTCCTGCAACGCGACGTGCACTACAT CGTCCGCGACGACGCGGTGCACCTGATCAACGCGTCGCGTGGCCGTATCGCGCAACTGCAGCGCTGGCCG GACGGGTTGCAAGCTGCGGTCGAGGCCAAGGAAGGTATCGAGACCACGGAAACTGGGGAAGTGCTCGACA CCATCACGGTGCAGGCCCTGATCAACCGGTATGCGACTGTGTGCGGAATGACGGGAACCGCGCTGGCCGC CGGTGAGCAGCTACGGCAGTTCTACCAGCTCGGTGTCTCACCGATACCACCGAACAAGCCAAACATCCGC GAGGACGAGGCCGACCGGGTCTACATCACCACTGCAGCCAAGAACGACGGGATCGTCGAGCACATCACCG AGGTGCACCAGAGGGGGCAGCCTGTGCTGGTCGGTACCCGCGACGTGGCCGAATCCGAGGAACTGCACGA ACGCCTGGTGCGCCGCGGTGTGCCCGCCGTGGTGCTCAACGCGAAGAACGACGCCGAGGAGGCCCGGGTC ATCGCCGAGGCCGGCAAATACGGCGCGGTCACGGTGTCAACTCAAATGGCCGGGCGCGGCACCGACATCA GGCTCGGCGGGTCCGACGAAGCTGACCACGACAGGGTCGCGGAATTGGGCGGCCTGCACGTGGTCGGCAC TGGCCGTCACCACACCGAGCGGCTAGACAACCAGCTGCGCGGTCGGGCCGGGCGCCAGGGAGATCCCGGG TCGTCGGTGTTTTTCTCAAGCTGGGAAGACGATGTCGTTGCGGCCAACCTCGACCACAACAAGCTGCCGA TGGCAACCGACGAAAATGGCCGGATTGTCAGCCCGAGGACGGGTAGTCTGCTCGACCATGCCCAGCGCGT TGCCGAGGGCCGGTTATTGGATGTGCACGCCAACACGTGGCGCTACAACCAGCTGATCGCCCAGCAGCGC GCCATCATCGTCGAACGGCGTAACACGTTGTTGCGCACCGTAACCGCGCGTGAGGAACTCGCCGAACTGG CGCCTAAGCGGTACGAGGAGCTGTCCGACAAAGTATCCGAGGAACGCCTCGAGACGATTTGTCGGCAGAT CATGCTGTATCACCTCGACCGTGGCTGGGCCGATCACCTGGCGTATCTGGCCGACATCCGGGAGAGCATC CATCTACGCGCGCTGGGCCGGCAGAACCCACTCGACGAGTTTCACCGGATGGCTGTGGACGCGTTCGCGT CGCTGGCCGCCGACGCCATCGAGGCGGCTCAACAGACGTTCGAAACCGCGAACGTCCTTGACCACGAGCC GGGGCTGGACCTGTCCAAACTGGCCCGGCCGACGTCGACATGGACCTACATGGTCAATGACAACCCACTG TCCGATGACACGCTTTCTGCCCTCAGTCTGCCCGGGGTGTTCCGCTG >gi|15608958|ref|NP_216337.1| preprotein translocase subunit SecA [Mycobacterium tuberculosis H37Rv] MNVHGCPRIAACRCTDTHPRGRPAFAYRWFVPKTTRAQPGRLSSRFWRLLGASTEKNRSRSLADVTASAE YDKEAADLSDEKLRKAAGLLNLDDLAESADIPQFLAIAREAAERRTGLRPFDVQLLGALRMLAGDVIEMA TGEGKTLAGAIAAAGYALAGRHVHVVTINDYLARRDAEWMGPLLDAMGLTVGWITADSTPDERRTAYDRD VTYASVNEIGFDVLRDQLVTDVNDLVSPNPDVALIDEADSVLVDEALVPLVLAGTTHRETPRLEIIRLVA ELVGDKDADEYFATDSDNRNVHLTEHGARKVEKALGGIDLYSEEHVGTTLTEVNVALHAHVLLQRDVHYI VRDDAVHLINASRGRIAQLQRWPDGLQAAVEAKEGIETTETGEVLDTITVQALINRYATVCGMTGTALAA GEQLRQFYQLGVSPIPPNKPNIREDEADRVYITTAAKNDGIVEHITEVHQRGQPVLVGTRDVAESEELHE RLVRRGVPAVVLNAKNDAEEARVIAEAGKYGAVTVSTQMAGRGTDIRLGGSDEADHDRVAELGGLHVVGT GRHHTERLDNQLRGRAGRQGDPGSSVFFSSWEDDVVAANLDHNKLPMATDENGRIVSPRTGSLLDHAQRV AEGRLLDVHANTWRYNQLIAQQRAIIVERRNTLLRTVTAREELAELAPKRYEELSDKVSEERLETICRQI MLYHLDRGWADHLAYLADIRESIHLRALGRQNPLDEFHRMAVDAFASLAADAIEAAQQTFETANVLDHEP GLDLSKLARPTSTWTYMVNDNPLSDDTLSALSLPGVFR Virmugen Vaccination of mice and guinea pigs with a secA2 mutant significantly increased resistance to M. tuberculosis challenge compared with standard M. bovis bacille Calmette-Guérin vaccination [Ref510:Hinchey et al., 2007]. In another study, lysine auxotrophy combined with deletion of the SecA2 gene made a live attenuated vaccine for Mycobacterium tuberculosis. SCID mice was highly protected from challenge after the vaccination [Ref1809:Hinchey et al., 2011]. sigE Mycobacterium tuberculosis H37Rv 888751 15608361 Rv1221 BX842575 NP_215737 83332 1364412 1365185 ? RNA polymerase sigma factor SigE 4.82 27091.02 257 Member of the extracytoplasmic function sigma factors which are active under specific conditions; binds with the catalytic core of RNA polymerase to produce the holoenzyme and directs bacterial core RNA polymerase to specific promoter elements to initiate transcription; in M. tuberculosis this protein is involved in heat shock, oxidative stress and virulence >gi|57116681:1364412-1365185 Mycobacterium tuberculosis H37Rv, complete genome CATGGAACTCCTCGGCGGACCCCGGGTTGGGAATACGGAATCGCAACTTTGCGTTGCCGACGGTGACGAC TTGCCAACTTATTGCAGTGCAAATTCGGAGGATCTCAATATCACGACCATCACGACCTTGAGTCCGACCA GCATGTCTCATCCCCAACAGGTCCGCGATGACCAGTGGGTGGAGCCGTCTGACCAATTGCAGGGCACCGC CGTATTCGACGCCACCGGGGACAAGGCCACCATGCCGTCCTGGGATGAGCTGGTCCGTCAGCACGCCGAT CGGGTGTACCGGCTGGCTTATCGGCTCTCCGGCAACCAGCACGATGCCGAAGACCTGACCCAGGAGACCT TTATCAGGGTGTTCCGGTCGGTCCAGAATTACCAGCCGGGCACCTTCGAAGGCTGGCTACACCGCATCAC CACCAACTTGTTCCTGGACATGGTCCGCCGCCGGGCTCGCATCCGGATGGAGGCGTTACCCGAGGACTAC GACCGGGTGCCCGCCGATGAGCCCAACCCCGAGCAGATCTACCACGACGCACGGCTGGGACCTGACCTGC AGGCTGCCTTGGCCTCGCTGCCGCCGGAGTTTCGTGCCGCGGTGGTGCTGTGTGACATCGAGGGTCTGTC GTACGAGGAGATCGGCGCCACACTGGGCGTGAAGCTCGGGACGGTACGTAGCCGGATACACCGCGGACGC CAGGCACTGCGGGACTACCTGGCAGCGCACCCCGAACATGGCGAGTGCGCAGTTCACGTCAACCCAGTTC GCTG >gi|15608361|ref|NP_215737.1| RNA polymerase sigma factor SigE [Mycobacterium tuberculosis H37Rv] MELLGGPRVGNTESQLCVADGDDLPTYCSANSEDLNITTITTLSPTSMSHPQQVRDDQWVEPSDQLQGTA VFDATGDKATMPSWDELVRQHADRVYRLAYRLSGNQHDAEDLTQETFIRVFRSVQNYQPGTFEGWLHRIT TNLFLDMVRRRARIRMEALPEDYDRVPADEPNPEQIYHDARLGPDLQAALASLPPEFRAAVVLCDIEGLS YEEIGATLGVKLGTVRSRIHRGRQALRDYLAAHPEHGECAVHVNPVR Virmugen A sigE mutant is highly attenuated in mice and induced significant protection from challenge with wild type M. tuberculosis, more so than the existing vaccine BCG [Ref1668:Hernandez et al., 2010]. sigF Mycobacterium tuberculosis CDC1551 922492 15842877 MT3385 AE000516 NP_337914 83331 3663004 3663789 - RNA polymerase sigma factor SigF 6.53 26809.27 261 Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; this sigma factor is a general stress response regulator; expressed in stationary phase and under nitrogen depletion and cold shock >gi|50953765:3663004-3663789 Mycobacterium tuberculosis CDC1551 chromosome, complete genome GCTACTCCAACTGATCCCGTAGCCGTGCCAATGACTTGGCCAGCAGCCGCGACACGTGCATCTGTGAGAT ACCGACGCGCTCGGCGATCTGCGTTTGGGTCATCGAGTCGAAGAACCTGAGCACCAAGACCGTTCGTTCC CGCTCGGGCAACGCCTCGAGCAACGGACGAAGCACCTCCCGATTCTCGATCTGGTCAAGACCCGCATCCA CGTCGCCCAGGGTGTCTGTGATTGCGCGGGCATCGTCGTCGCTGCCGCCACCGCTGTCGATGGACAAGGT GTGGTAGGAACTACCCGCCAGCAAACCTTCGATAACCTCAGCGCGGTCCATCCCGAGCTCCGCGGCGAGC TCCGATGCCGACGGCGCCCGCCCGAGCCGCTGCGACAAATCGGCGGTGGCGGTACCTAGCCGCAGATGCA GTTCCTTGAGACGCCGGGGAACCTTGACCGACCAGCTGTTGTCGCGGAAGTGTCGTCGGACCTCGCCCAT GATGGTAGGAACCGCGAAGGAGACGAAGTCCGACCCGGTCTTCACGTCGAAGCGAACCGCGGCGTTGACC AGCCCGACCCGCGCGACCTGAATAAGGTCGTCACGCGGTTCGCCGCGACCCTCGAACCGCCGCGCGATGT GATCGGCCAGCGGCAAGCACCGCTGAACGATCTTGTCCCGGTGCCGCTGGAATTCCGGTGAGCCGGCAGG CAAACCAACCAGCTCGCGAAACATCTCCGGAACGTCGGCGTATTCGTTAGCTCGCGATGCAGAACCGCCG GCAGCGCGCGCCGTCA >gi|15842877|ref|NP_337914.1| RNA polymerase sigma factor SigF [Mycobacterium tuberculosis CDC1551] MTARAAGGSASRANEYADVPEMFRELVGLPAGSPEFQRHRDKIVQRCLPLADHIARRFEGRGEPRDDLIQ VARVGLVNAAVRFDVKTGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRLKELHLRLGTATADLSQRLGR APSASELAAELGMDRAEVIEGLLAGSSYHTLSIDSGGGSDDDARAITDTLGDVDAGLDQIENREVLRPLL EALPERERTVLVLRFFDSMTQTQIAERVGISQMHVSRLLAKSLARLRDQLE Virmugen A sigF mutation is highly attenuated in Mycobacterium tuberculosis and induced protection from challenge in mice[Ref2113:Geiman et al., 2004]. SLC11A1 Homo sapiens 6556 109255241 AC021016 NP_000569 9606 2 219246751 219261616 + solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 7.72 56200.3 550 Also known as LSH; NRAMP; NRAMP1 >gi|224589811:219246751-219261616 Homo sapiens chromosome 2, GRCh37.p5 Primary Assembly CACAGAACACGGGGTGCCTGGAAGGGGAACAGATGTGTTGTGGGGCACAGGGCAGGCTGGGAGGGGAACA AAGGTCCACTCCATGGGTAACCAGACCCTTCCGCCAGGGCTGGCCACTTCTGCCTTTGGAAAATGTTTCA CAACGCCCCATGTTGTGTGTGTGTGTGAATCGGCCGATGTGAACCGAATGTTGATGTAAGAGGCAGGGCA CTCGGCTGCGGATGGGTAACAGGGCGTGGGCTGGCACACTTACTTGCACCAGTGCCCAGAGAGGGGGTGC AGGCTGAGGAGCTGCCCAGAGCACCGCTCACACTCCCAGAGTACCTGAAGTCGGCATTTCAATGACAGGT GAGTAGTGGCCCCTAGGGACAGAGCCTGATTGGGGGGTGGAGTGGAGGAGATCACTAGGCTGGTGGAGAC TTGAGGAAGCAAGAAAGCCCTTGGTCCCCTGTGGAAGCCTCTGGTCCCCCGTGGGAGTCCTAGTCTAGAA CACAGTTCTGGACACCAGACTCAATCTCACTGGGTGGAGTCCTTCACACCCCTAAGCGGTCTACCTTTCC TTTGTCTGAAGTCCGTGGATCAAAGCCCCTGGAGCTTTCTGGGCTGCCGGAGAAGGTGAGGGGTCCAGCC AAGGGGCAGACATGTGGCCTCAGCTTGCCCTTCTTAGAGCCTAGGGTCTCTCTGCATTCTCTGTGGCAGG AGATCTGCCTCTGGGCATCTGTCCTCACATGAGAGGACAGTAATTCTGAAGCTGCCATGTCCCACACATC TGGAATGTTTGTTTGAGAGGTGGAGGTGATGGACCCAAGATGGGGGCCAGGGACCTTAGTTTCTCCACTT TTCCGGGAGGAGGAGGGAAAGGATCAGGCGGCTTTAACTCTGGGCCACCAGAAAGAGGCAGGACACTCAC CATGCTTCATGGGCCCCCACAGGTGACAAGGGTCCCCAAAGGCTAAGCGGGTCCAGCTATGGTTCCATCT CCAGCCCGACCAGCCCGACCAGCCCAGGGCCACAGCAAGCACCTCCCAGAGAGACCTACCTGAGTGAGAA GATCCCCATCCCAGACACAAAACCGGTGGGATGCTGGAAACTTCCTGGGGGCTTGCAAGATTGACAGGAT CCTGAAGGATCCCATGGGCTGGAGAATGGGGTCTCCTTATGATCATGGGTGGCAAGTCCCTTCCAGGTCT GAGCAGCAGTGGTGGACAGTGGCAACTGCCCCTTCCTGCTTCCGTCCCCAGTGCTTAGCTGGGGTGCTTC AGTATTTGTCTAAAAGCGATTAAATGACTTTCTCAATGAATCTCAGCTTTCCTATGAAGGAAAAGAGGCC CAGGGGGAATTGGGATTGGAGCCTTGAGGACATGTTATAGCATTTAGGAAAGGGCATAGGGAGAAGTGAA GGAGGCTGCAGGCTGGCATCTAGCATCTAGGATGCTGTCACCTGCATTTCCAGTATCTTCTCTTTTTTTG GAGATGGAGTCTCGCTCTGTAGCCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAACCTCCGC TTCCCAAGTTTAAGCGATTCTCCTGCCCCGGCCTCCCGAGTAGCTGGGATTACAGGCATGCACCACCATG CCAGGCTAATTTTTGTATTTTTACTAGAGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCT TAGCTCAAGTGATCCACCCACCTCGGCCTTCCAAAGTGCTGGGATTACAGACACGAGCCAACGCTCCCAG CCTCCAATATCTTCAGCAGATTTCTAAGTCAATATGCCATGCTCTGGAACAGGGTGGGGGGACCATAGCC TCAGCGGACCACAGAGAGGAGGCCCCGTGGTCTGAAAAGGAGCTGGCAAGGGAGGAGCTGGGCTAGGGAA GCTTGGCCCCCTCACCCCCTTAGACAGCCCCCTGTGAGGTCTCTGAGGACCTGACAAGTCAGGGCTTGAG GGGCAGGCAGTTCAGGTGCAAGCCGGCTGCTGAGCTTAACAGATCCAGAAAAAGATCCTCTGTGACTCAT CAGAGCATGCTGCTTCCTCCACCCATGCCCTCCCCATCCCTTGGGTGGCAGACCCAGGAATGGGCCATGG AGGGCAGGGCTGGGCTGATGAGCCTGTTGGGGCTCCTCTAGGGGATGGCCAAGGCCAGCTGCCACCATCC CTATACCCAGGACCCCCTCACTCTACTCCTCCCACCCCCCAACAGGGCACCTTCAGCCTGCGGAAGCTAT GGGCCTTCACGGGGCCTGGCTTCCTCATGAGCATTGCTTTCCTGGACCCAGGAAACATCGAGTCAGATCT TCAGGCTGGCGCCGTGGCGGGATTCAAAGTAACTAAGTCGGGACCTGAGTGGGGACACTTTCGAGAGGGA GGTGACCAGGCTAAGTGTTGAAGGCCCCTGCTGGCCATGTTTCTCCAATGTGGCCTGGGAGCTGGTGTTA AGTTCAAATGGGCCCGAAAAGGGTCCCCAGGGCAGCCCTGCCAGAAGGTGGGGCATTTGTGTGGGGGGTA GGGGACTGGACTCCTCTCTTTAAAATATATATATGTATATTTATTTATTTATTTATTTAATTTTATTTTT TGAGACTCAGTCTCACTCGGTCACCCAGGCTGGAGTGCAGTGGCACAATCTCGGCTCACTGCAACCTCTG CCTCCCGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGTGCATGCCACCACGCCTGGCTAATTT TTGTATTTTTAGTAGAGATGGGGTTTCACTGTGTGGGCCAGGCTGGTCTTGAACTCCTGACCTCAAGTGA TCCACCGCCTCGGCCTCCCAAAGTGCTGGGATAACAGGTGTGAGCCACCTTGCCTGGCGTAATTTATTTA TTTATTTAGAGATGGGGGTCTCACTATGTTGCTCAGGCTGCTCTTGAACTCCTGGACTCAAGCAATCCTC CCACCTTAGCCTTTTAAAGTGCTGGGATTACAGGGTGAGCTACCAGCGCCCAGCCAGGATGGGACTCCAT CTCTCCCACCCCCTCCCCGAGGAGTATGCTTGGTTAGAGGGTACCACGAGCTCAGGGGCTTTCTGAGGCT GGCCTCTCTGGCTGAAGGCCTCTCCCTGCCTCCTCACAGCTTCTCTGGGTGCTGCTCTGGGCCACCGTGT TGGGCTTGCTCTGCCAGCGACTGGCTGCACGTCTGGGCGTGGTGACAGGCAAGGACTTGGGCGAGGTCTG CCATCTCTACTACCCTAAGGTGAGCTTGGGGGGCCTGGACAGGGAGAACCACTGGCCCCAAACCCCAAAC AGCCATTTTCAGCTTCCACGATCAAATAAATACATCATCTCACATGGGGCATCCCAAGTCTGTTTGTTTT TGTTGTTGTTTGTTTGTTTTTCAGTCGGAGTCTCGCTCCGTCGCCCAGTCAGGAGTGCAATGGTGCGATG TCAGCTCACTGCAACCTCTACCTCCCAGGTACAAGTGATTCTCCTGACTCAGCCTCTCAAGTAGCTGGGA TTACAGGCGCCCACCACCATCATCGGCTAATTTTTGTATTTTTAGTAGAGCTGGGGTTTCACCATGTTGG CCAGGCTGGTCTCAAACTCCTGACCTCAGATGATCCACCCACCTCAGTCTCCCAAAGTGCTGGGATTACA GGCATGAGCCACTGCATCCGGCCCCAATTCCATTTGATAGATGGAAAGACTGAGGCTCAAACTGATAGCA CATAGCCTAGAGGGACATGAGAGGCATGTCATTTCACAGAAGGGGTAATTAAAATTTGGGAAAGAGACTT GCCCAAGACAAAAAATGGGGAGGCATCAGCTCAGGAATGGTCACTGGTTGGGGTGGACCCTGGGGACTTC GTTGTTGTGAGTGTCCAGTTTGGAAAACAGAAATTACAGCACTGGACATTTCAAAGGTATTGAATGCAGG GATTGAAAGGTTTGGGAGGGCTTGAGGGGCAAGGGAAGAGGGTGTTACAAAAAGATCAGAAAGCTGCTAC ACCTCTGGCACAAGCCCCAGAGCCATCCTTTGCTGCTGAGCTCCTGGAAGCATTATTGATGCTGATGAAA TGGCCACTAGTGCTGCCAGAGTCCGGGTTGCCTGGTGCCACTGCCAGAAATACAAGAAGCAGGGGGAGAA AATGGTTTATCTCAGCCTGGGGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGT GGATCACCTGAGGTCGGGAGTTCGAGACCAGCCTGACCAACATGGAGAAACCCCGTCTCTACTAAAAATA CAAAACTAGCCGGGCATGGTGGCACATGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCG CTTGAACACGGGAGGCGGAGATTGTGGTGAGCCGAGATCATGCCATTGCACTCCAGCCTGGGCAACAAGA GCAAAACTCTATTTCGAAAAAAAAAAAAAAAGTTTATCTCTGCCTTTCCATCTCCTGTATGCTCTTCCTA CATAAGGTAGGAAGCCCATTGGCCAGACTGTCTAGTAAATGTAGTCTGAGACGACAGACAAATGTAGTCT GAGACGACAGGCAAATAACCGGCCCACCCTTAATGAAGGATCATCTCCTCCCCATAGGTGCCCCGCACCG TCCTCTGGCTGACCATCGAGCTAGCCATTGTGGGCTCCGACATGCAGGAAGTCATCGGCACGGCCATTGC ATTCAATCTGCTCTCAGCTGGACGGTACCACCCCAGTGTACCCCAACTCTTCAGGCCAGGCAGAGAACAG CTGCTGCTACTTCCCCCCCTAACCAGTCCCTCCCAGAGTCTATTTTATCCTGCTGTCCCCTCTGAAGCAG GGCTGCTGCCCTGTTTTCCAGAAATGTAAAGTGACTTGTCTAAAGTCACACAGATGTGAGTCATGCAGGA CTTTGGGACTGCAGCCCCAAACTCCCTGCTGCGCCGGGTGCCAGGTCTCTCCTCTAGCTCTGCCCTGCCT CGACTGTTCTATGCCACACTCCCACTCCCCTTGCCCTAGCTGTCTGGGGGCGCTTAGGGTCCTGCTCCCA GGAGGCCAGATTCCTGTCTCCAGCCCTGAGGCTCCGTAGGAGTTAGAGACCCCTGGACCAGGCTGGGCTG ACCCGGGCCACTCTGGTTTCAGAATCCCACTCTGGGGTGGCGTCCTCATCACCATCGTGGACACCTTCTT CTTCCTCTTCCTCGATAACTACGGTGGGTGCACACCCCACCTCATAGGGGAGTGGTGGTGGTGAGGGTGC TGTACTGGGAGAAGGGCTCTGACATCGAACAGCCTGGGAGCGCACCTGAGCTCCCTCACTCTCCCTGGGT GGCCTCTAGCGAGTTACTTGGACGTGCCTCTCTTCACCTGTAACATGGGGAAATAATAGCACAGACTTCA GAGGGTGGTTGTGAAGACTGAGTGAGGTAACATATGTTAAGCACTTGGCACTGTGCCTAGAAGCGCTCGT AGTATTAGCCATTACGAATTGTTGATGTCACAGATTTTTTTCGTTGGTTTGTTTAGTTTGTTTGTTCTGC TCCGTAGGGCTGCGGAAGCTGGAAGCTTTTTTTGGACTCCTTATAACCATTATGGCCTTGACCTTTGGCT ATGAGGTAGGAAGCCAGTGCTGCAACCCCACTGTGGACCTCCCAAGATCATTCCTCTCCCTTCCCTCTGG CCGCGGGCTGCGGGGGGCTGGGGTGGGATGGAGGCTGAGAAATGGTGACCGCGGCGTGGTTGCGAGGGGC GGGGCTTGTCCTGACCAGGCTCCTCCCTGCAGTATGTGGTGGCGCGTCCTGAGCAGGGAGCGCTTCTTCG GGGCCTGTTCCTGCCCTCGTGCCCGGGCTGCGGCCACCCCGAGCTGCTGCAGGCGGTGGGCATTGTTGGC GCCATCATCATGCCCCACAACATCTACCTGCACTCGGCCCTGGTCAAGGTGAGCAGAGGGGAGGGGAAAG GAGACCCCCTCACTCAGTCGGAGCCATGCTGGCTCCGCCTCCAAGCCTGGAGCCCCTCCCCTCTGGCTCC TTCCCTCAGGATTTGCGGAGCCTGTAATAATTGAGGGACAGGCGGGGCGCCATGGCTCACGCCTGTAATC CCAGCACTTTGGGAGGCGGAGGCGGGCGGATCACGAGGTCAAGAGATCGACACCATCCTGGTCAACATGG CGAAACCCCGTCTCTACTAAAAATACAAAAACTAGCTGGGCGTGGTGGCGCGCGCCTGTAGTCCCAGCGG CTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCAGAGGTTGCGGTGAGCCGAGATCTTGCC ACTGCACCACTCCAGCCTGGCGACAGAGCGAGACTCTGTCTCAAAAAAAAAAAAAAAAGAAAAGAAAAAG TGTCTACTGTGCGCTGGGAGGAACAGACCGTCAAGGCCTCTGCCCTTCTAGAGTTTCCGTGCTAGTGGGG TAGACGGATGACAAGCAAGTAAACCAACAAAGGAAAGAATTATAGGCCGGGCGCAGTGGCTCACGCCTGT AATCCCAGCACTTCAGGAGGCCGAGGCGGGCGGGCGGATCACGAGGTCAGGAGTTTGAGACCTCAACAGC CTGGCCAACATGGTGAAATCCCGTCTGTACTAAAAATACAGAAAATTAGCCGGAAGTGGTGGCGCGTGCC TGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAAGCCAGTAGGCGGAAGTTGCAGTGA GGGGAGATGGTGACATTGCACTCCAGCCTGAGTGACAAAGCAAGACTCCGTCTCAAAAAAAGAAAAAGAA CAAGAACTATAGAGCTGGGCGAGGTGGCTCACGCCTGTAATCCCGGCACTTCAGGAGGCAGAGGCAGGTG GATCGCTTGAGCCCAGGAGTTCGAGACCACCCTGGGCAACATGATAAAACCCTGTCTCTACCAACAAAAA ATAAAAAATAAAATAAATTAGTTGGGTGTTGTGCACACCTGTAGTCCTAGTTACTCAGGAGGTTGAGGCT TGAGCCCTGGAGGTCGAGGCTGCAGTGAGCTGAGATCCCACCACTGCACTCTCAGTGACACAGTGAGACC CTGTCAAAATAATAATAATAATAATAATAATAATTGCGAAAGGGACTCTGAAGTAAACAAAACAGAGAAA TATGATAGAGAATGACTGAAAGTTGGGGAAGTAGATCAGGGAAAAGATATTACAAGCGGAGGGAAAGCAA GTGCAAAGGCCACTGGCAGAAGTGAACAAGGCTTGGGTCCCTAACAGCTTAACAGTGGCTCACACCTGTA ATCTCAGCATTTTAAGAGGCCAAGACAGGAGAATGCTTGAGCCCAGGAGTTCAAGGCTAGCCTAGCAAGA CCCTGTCTCTGCAGAAAATTTAAAAATTAGCGGGTTGTAGTGGTGTGTGTCTGTGGTCCCAGCTACTTAG GAGGCTGGGGCAGGAGGATCACTTGGGCCCATGAGTTGGAGGCTGCAGTGAGCCATGCATTGCACCACGG CACTCCAGTCTGGGTGACAGAACAAAACCTGTCTCTAAAAAATAAAATAAAGTAAGCTGGACACGTCTGA GGATGGAACAAGGTGAGTGAAGGAGCGTGTCAGGACCTGAGGTAGCCAGGGACCTCAAAGGCCAGCCTTG CTTCACCCACACAGTGGCTTACAGTGGTAAGGCCTCTGTGGCAAGAACAGAGATGTAGGAAACCATCGGC TGACCTGAACCTGCCCCAGACTGCCACGCAGGGGACTTAAGAAGGTACTGGGCTTTGGGGAGAACATAGA AGTGTGAGGGTGGGGGACACTGTGGTGGCTCTGAGGGACTTTGGCACTTCCCTCTCCCTTTGATCTTCGT AGTCTCGAGAGATAGACCGGGCCCGCCGAGCAGACATCAGAGAAGCCAACATGTACTTCCTGATTGAGGC CACCATCGCCCTGTCCGTCTCCTTTATCATCAACCTCTTTGTCATGGCTGTCTTTGGGCAGGCCTTCTAC CAGAAAACCAACCAGGCTGCGGTGAGACACACTTTCCCCCGCACCTGAGGCCACACACGTACTCATGTCC TGTAAGCCTTGCCGAGGACCCTAGGCAATGCAGCTGAGCCCTTCTGAGTCTCTGCCCTGATGATCTTCCC TGTTGGCAGATATCATTCATTCAGCAAATAATCATTGAGCATTTGTTATATACCAAGCACATCCTAGACC CTGGGGATACAGCAGTCAATGCTACAAAGACCCAGCTCTCTGCAGCTTAAATAGGGAGGGAGGTGAGCAG ACAGGAATGAATACCCAAGGGAAGTACAGAGTGGGAAGAAATAAAGGGGGTGAAGAAGCATAGTTGGGGG TATGGGGGTGGGGTCTGCATTAGCCCAGGTGGTCAGGAAAGGCTTCTTGGAAGAGATGACATTTGTGCTA ATACTGGAAAAGGAACTAGCCAGCTCCTCCCTGACTCTCTGGGATCTGGGACCTGTGTCTGTCTCCCCTT CTGAGTGGATCTCAGTCATGAGGCTCTAACAACTGCAGCCAGGGTTGGGGGCTTCCTGAATGCTGAGCCC TGTGGGAGAAGGGGTGGCCAGGCCTCCAAGGACCTGGGCACTGCCACTGCCTTGCTCTGTGACCTTGGGA GGTCACACTCCTCCCTGGGTCTCACTCTGTTCCTTGGCACAAGGAGGGAGATCAACATCAGGACTGCAAA CCCTTGTCTTCAAGACCAGGCAGGCGCAGAGTTGTGGGAAGTGGACAGTGTGAAGAGGGCATGGCAGCCA GCTGTCTCTGTGGTGATGCCAGCTCTTACGAACCTAAAAGAAGCTGGAAACTGGGTTCTTATGCCAGGCC TTCTCAATTTTAAATGTTGGCAACAAATCCACATTAAAGAAAAAAAAAACCCGGCCGGGTGCGGTGGCTC ACGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGATGGCTCGCTTGAGCCCGGGAGTTCAAGACCAA CCTGGGCAACATGACAAGACCCCCATCGCTACAAAACGTAAAAGTAAAACAAAACAAAACCCAACTTATT TTGTTAGCCAAACAAAATATGTCTGGCCAGATTCAGCCTTGGGCCACCAGTTTTGGAACCCTGGTCAGTG CTAGGCAGTCCAGTTTCCCAAGGCTGAGCGTGCTCCTCACATCTTCCTTCTACTGCCCTGGTACCCACAG TTCAACATCTGTGCCAACAGCAGCCTCCACGACTACGCCAAGATCTTCCCCATGAACAACGCCACCGTGG CCGTGGACATTTACCAGGGGGTGAGCGCGGGTGGGTGGGGAGGGCGTGACCCAGAGAGGCTCCCCGCCTC GGGCAGGGCCACCGGTCCTACCACACTCGTCCCTGCAGGGCGTGATCCTGGGCTGCCTGTTCGGCCCCGC GGCCCTCTACATCTGGGCCATAGGTCTCCTGGCGGCTGGGCAGAGCTCCACCATGACGGGCACCTACGCG GGACAGTTCGTGATGGAGGTAGGGCAGGGGGCGGGCCCAGGAGGGCAAGGGGTCCAAGGACAGCAGGCAC ACTACTGGGGGCTAGAACTCCGAGCCTTGTGGGTCCTCTTTTCTTTTCTTTCTTTTCTTCCTTTTTTTTT GTTTTTGTTTTTGTTGTTGTTGTTGAGACGGAGTCTCGCTCTTGTCGCCCAGGCTGGAGTGCAGTGGCGC GATCTCGGCTCACTGCAACCTCCCCCTCCCAGGTTCAAGCTTCTCCTGCTTCAGCCTCCTCAGTAGCTGG GATTTCAGGCACCTGCCACCAGGCCTGGCTAATTTTTGTACTTTTAGTAGAGACAGGGTTTCGCCATGTT GGCCAGGCTAGTCTTGAACTCTTGACCTCAAGTGATCCGCCTGCCTTGGCCTCCCAAAGTGCTGGAATTA CAGGTGTGAACCACCGCACCCGGCCAATTTTTGTATTTTTAGTAGAGACGCAGTTTTGCTGTGTTGGCCA GGCTGGTCTCAAACTCTTGACCTCGTGATCCGCCCGCCTTGGACTCCTGAAGTGCTGGGATTATAGGCGT GAGCCACTGCGTCCGGCCTTTTCTTTTCTTTCTTTCTTTCCTTTTTTTTGAGACGGCGTTTCGCTCTTAT TGCCCAGGCTGGAGTGCAATGGCGCGATCTCCGGCTCACCGCAACCTCCACCTCCTGGGTTCAAGCAATT CTCCTGCCTCAGCCTCCTGACCAGCTGGGATTACAGGCCACCACACCCAGCTAATTTTGTATTTTCAGTA AAGACGGGGTTTCTCCATGTTGGTCAGGGTGGTCTCGAACTCCCAGCCTCAGGTGATCTGCCCTCCTCAG CCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCTCTTTCTTTCTTTTTTGAGACAGGA TTTCACTCTGTTGCTCAGGCTGGAGTGCAATGGCGCCATCACAGCTCGCTGCAGCCTCAACTTCAGCCTC CCGAGTCATTGGGACTACAGGCTCATGCCACCACACCAGGCTAATTTTTAAAATTATTTGTAGAGATGTG GTCTTCCTATGTTTCCCAGGCTGGTCTCGAACTCCTGGGCTCAAGTGATCTTCCCGCCTTGGCTTCCCAA AGTGCTGGGATTACAGACGTGAGCCACCCCACTCAGCTTTGTTTCCTTCATGTAGCCCAGAGGGCGACAC TATAAGCCCCATTTTACAGATGAGAAAACAGATCCTGACAGATGAGGTGACTTGCTGGAAGCTGCAGACC CAGGACTAACAGAAGCCTTTTCCCCAAAACTGTTTAGTCTTCAGCAACCAGCTATGGGATGGGAGCTCCC CATTTCTCCCCACCCATCCCCTCTTGCCACCTAGGGACAGAGCTGTCCCAGTTCAACAGTGGAAAAACAG AGCATGCCCCCAGGGATAAATCGGTTGAGGGACTACAGAGGATCTCTCCTCTGGAATCCCCAGTCCTGTC TACTCCTCACCAAGGAGTTCACCCCCACCCCAGGGCTTCCTGAGGCTGCGGTGGTCACGCTTCGCCCGTG TCCTCCTCACCCGCTCCTGCGCCATCCTGCCCACCGTGCTCGTGGCTGTCTTCCGGGACCTGAGGGACTT GTCGGGCCTCAATGATCTGCTCAACGTGCTGCAGAGCCTGCTGGTGAGATGCGCCAACCCCACCAGCCCT GCCCACTGCTGAGCGAAACAGGACCTCCAGCAACCCCCACGCTGAGCCTTGCATGGGCCTGCCAGGCCCT GTCCCAGGCACATCTTGCCTACAAGGCTTCCATTGTCCCCACAGCCACCCTGGGGGGCAGGAGGGTAGGA ATCACCATTATCCTTCCCCCCTCCCACCCCCCAGATGGTCTCCCTCTGTCACCTAGGCTGGAGTGCAGTG GTTCAATCATGGCTCACTGCAGCCTGCCCTCCCTGTCTCTAGCAATCTTCCCACCTCAGGCTCCCGAGTA GCAGGGACTATAGGTGCATGCCATCACACCCAGCTAATTTTTTTTTTTTTTTTTTTGAGACGGAGTTTCA CTCTTGTTACCCAGGCTAGAGTGCAGTGGTGAGATCTTGGCTCACTGCAACCTCTGCCTCCCAGGTTCAA GCGATTCTTCTGCCTCAGCCTCCTAAGTAGCTGAGATTACAAGCGCCCACCATCACACCCAGCTAATTTT TTGTATTTTTAGTAGAGACAGGGTTTCATCATGTTGGCCAGGCTAGTCTCAAACTCCTGACCTCAGGTGA TCCACCCGCCTTGGCCTCCCAAAGTGCAAGGATTACAGGCATGAGCCACTGAGGCAGGTCTAATTTTTTT TTTTTTTTAAGTAGAGACAAAAGCTCATTATGTTTGTTGCCCAGGCTCATTATTCCATTTTACAGATGGG AAAACTGATGCACTCAGTTAAGTTACTAGTGGCAGGGCTTGGATTCAAACCGAAGCAACCTGGTGCCAGA GCCCCCAAACACTTTCTCCTGCTTTGGAGCCCACAACCTGATAAGGCAGGAGACGGGGAAGTAGGCTCAG TGTGGTTAGGGCAGTTGAGCTCACACCCACACAGAGGGTGTCAAGCCACGCCCATCTTGCCCCCACCCTT GCCATATTCTGAGGCAGAATTCCTCAGCCTAGGCTCCTGCTGCTCTCCCCAGCTCCCGTTCGCCGTGCTG CCCATCCTCACGTTCACCAGCATGCCCACCCTCATGCAGGAGTTTGCCAATGGCCTGTGAGTACCCCCTT TCCCAAGTGCTGGATTGCATCACCACATTTCATCCTCACAGCCACCCAGAGGTACGAGCTACAATTCTCC CCATTATCCCAATGAAGCCACAGAGGCTGAGAGAGGTTAAGGGACTTACCCAGGTTCACACAGCTAGCAA GTTGAGGAGCCAAGACTGGAATCCAGGGAAGAGGGCTGTTTCTAGAGCCTGAGCGCCTCACTCTCGCCTC AGACTCAGCTCTTGCCAAATCCCGCCAGTGTGTAGCCTGGAGGGCCCAGGAGGCAGGAGGTGGGGAGGGG GTCTGTCTGCTCCAGGTCCCACAGACCAGAGAAGCGGCCTCAGTGTATCCCCACCCCCAGTGTGGGCGCT GGGAGATGAAGAGGAGTTGATGCAGGTGGGCCAGCAGCAACCAGCCAGTCCTGAGCCTCTCTCGTGTCCC CCAGGCTGAACAAGGTCGTCACCTCTTCCATCATGGTGCTAGTCTGCGCCATCAACCTCTACTTCGTGGT CAGCTATCTGCCCAGCCTGCCCCACCCTGCCTACTTCGGCCTTGCAGCCTTGCTGGCCGCAGCCTACCTG GGCCTCAGCACCTACCTGGTACAGTAGGGCCAGGGGATGCCTTGGGAATGGATGAGGGAAGGACAAGAGG CAACCAATGGGGAGGGTTTGGGGGGACACAATGGGGCTTCCCCAGAGGTCTTGGCATCTCCCCAATTCAT GGTTGCCCCTCCCCCAGGTCTGGACCTGTTGCCTTGCCCACGGAGCCACCTTTCTGGCCCACAGCTCCCA CCACCACTTCCTGTATGGGCTCCTTGAAGAGGACCAGAAAGGGGAGACCTCTGGCTAGGCCCACACCAGG GCCTGGCTGGGAGTGGCATGTATGACGTGACTGGCCTGCTGGATGTGGAGGGGGCGCGTGCAGGCAGCAG GATAGAGTGGGACAGTTCCTGAGACCAGCCAACCTGGGGGCTTTAGGGACCTGCTGTTTCCTAGCGCAGC CATGTGATTACCCTCTGGGTCTCAGTGTCCTCATCTGTAAAATGGAGACACCACCACCCTTGCCATGGAG GTTAAGCACTTTAACACAGTGTCTGGCACTTGGGACAAAAACAAACAAACGAAAAACATTTCAAAAGGTA TTTATTGAGCACCTGCAGGCGTGACCTGACAGCCCAAGGGTGGGTGGGGTGAGGGCTTGAGGACTTGGGC GGGACACAGGCTCCAAACTGGAGCTTGAAATAGTGTCTGATGAATGTTAAATTATCTATCTATCTATTTA TTTATTTATTTGAGACAGGGAAAGGGTCTCCCTCTGTTGCCAAGGCTGGAGTGCAGTGGCGCAATCTTAA CTCATTGCAACCTCCACCTTCTGGGTTCAAGCGATTCTCTTTATTCAGCCCCGGGAGTGGCGCGCGCCAC CACGCCCAGCTAATTTGTGTATTTTCAGCAGAGACGGGGTTTGCCATGCTGGCCAGGCTGGTCTCGAACT GCTGGATTCAAGTGATCCGCCCATCTCCGTCTCCCAAAGTGCTGGGAATTACAGGCGTGAGCCACCAAAC CCGGCCTGATTAAAGTTAAATAAATACTAGTTCCCTTCTCGTCCAAAGGAGCAGGGAATGGGAACCGGGA AGGCACGAAGTCTCTAAAGCATCCAGAAGACCCCTACACCAGGGTCTGGTCCGCTCCTATTCGCCGCAGC CTTTCTGTTCCGCCTGCAACCCATTTTCCAGACAGTAAAACGGCGGCGCACTTCTTTCTCCGTCAGGCAC CAGGTCATAAGGAACCCAAGAGTCTGTGCCTCTGAGGCCCAAATTATTTGCTGTTTCCTCAGGGGAGCCG GCGGCCGCGACTCCCACGCCGCGCCGTTACCGCTCCCTCTCTGCTGACTGCTCCCCCTAGGGGCAGAGAC GGTCCCGACGCCCGCCATCCCGCCCCGGCCTCACCCCTCCCCGCCAGGCGGAACGACGCGGGGAGGCGGG CGCTCGGGGCTCGCGCCAGGGGCCCCAGAATCCTTCGGGGAGAGTGGGTGGGAGGAAGCTGTGTGGGCGG GGAGCCCCCTCTGCCTTAGGGAGCGGCTGGGCACCCATTCGCCCCATTCAGGGGCTGCACTTTATAGACG TTCCCTAGGCTGTTTCTAGGCTCCCCCAAGTCCCTCCTCCAGCCTCGTCGGGTCCCTCAGACCCCAGCCC AGGACCTGCGGAGGGCCGCAGCGAGGAGAGGCCAACAGGCCTTTCCCTAGAGTTGAACCTGGGCCGGGTG TTGCACCTGGAAGAACCCCCGATTTCCTGGGGACCCAGCAGGGCAGGCGGCCTGGCTCCGCGCTCAGGTC CGGACGCTTGTTTATGAGAAGAATTTCCTCTTTCTTAAAAGGGCAACGATGCGAGTGGGTCCCTCAAGGA GAGAAGAGATGGGACCGGTCTGGTGCGACCTGGGCAAGCGCTGCAGAGGGTACCTGGGCAAGAGGGCCGC CCGCCTCCTCTGGGTTTGGCACTGGAGAAGATGGGTCCATGCCAGCTGAAGGAGGAGATGGATGGGGGAC GTTTAGCGAAGAAAGGCATCTCCCAGATCCTTTAGCCTCCTGGAAGTGCCCCCGTTGTACCCCCTACACA CCCCTCTTGGCATTGAGTGCCAGTCCTCTGCCAGGCTCTGTGTTACAAGTTGGGGAGGGCGGCAAAGTCC CGAATTAAAGATGTCAGTTCTCAAGG >gi|109255241|ref|NP_000569.3| natural resistance-associated macrophage protein 1 [Homo sapiens] MTGDKGPQRLSGSSYGSISSPTSPTSPGPQQAPPRETYLSEKIPIPDTKPGTFSLRKLWAFTGPGFLMSI AFLDPGNIESDLQAGAVAGFKLLWVLLWATVLGLLCQRLAARLGVVTGKDLGEVCHLYYPKVPRTVLWLT IELAIVGSDMQEVIGTAIAFNLLSAGRIPLWGGVLITIVDTFFFLFLDNYGLRKLEAFFGLLITIMALTF GYEYVVARPEQGALLRGLFLPSCPGCGHPELLQAVGIVGAIIMPHNIYLHSALVKSREIDRARRADIREA NMYFLIEATIALSVSFIINLFVMAVFGQAFYQKTNQAAFNICANSSLHDYAKIFPMNNATVAVDIYQGGV ILGCLFGPAALYIWAIGLLAAGQSSTMTGTYAGQFVMEGFLRLRWSRFARVLLTRSCAILPTVLVAVFRD LRDLSGLNDLLNVLQSLLLPFAVLPILTFTSMPTLMQEFANGLLNKVVTSSIMVLVCAINLYFVVSYLPS LPHPAYFGLAALLAAAYLGLSTYLVWTCCLAHGATFLAHSSHHHFLYGLLEEDQKGETSG Vaximmutor On multivariate analysis, patients with the NRAMP1 (i.e., SLC11A1) D543N G:G genotype and allele 3 (GT)n polymorphism had decreased recurrence time (p=0.014 and p=0.03) after human BCG therapy. SodA Mycobacterium tuberculosis H37Rv 886174 15610982 Rv3846 NC_000962.2 NC_000962.2 EMBL: AE000516 EMBL: AF061030 EMBL: BX842584 EMBL: X52861 Gene3D: G3DSA:3.90.149.10 GenomeReviews: AE000516_GR GenomeReviews: AL123456_GR InterPro: IPR001189 KEGG: mtc:MT3960 KEGG: mtu:Rv3846 LinkHub: P17670 PANTHER: PTHR11404 PDB: 1GN2 PDB: 1GN3 PDB: 1GN4 PDB: 1GN6 PDB: 1IDS Pfam: PF00081 Pfam: PF02777 PIR: S15205 PRINTS: PR01703 ProDom: PD000475 PROSITE: PS00088 TIGR: MT3960 TubercuList: Rv3846 UniProt: P17670 1773 4320704 4321327 + SUPEROXIDE DISMUTASE [FE] SODA 6.43 23033.8 207 Rv3846, (MTCY01A6.22c), len: 207 aa. sodA (alternate gene names: sodB, sod), superoxyde dismutase (EC 1.15.1.1) (see citations below), equivalent to many e.g. P47201|SODM_MYCAV|SODA|SOD from Mycobacterium avium (206 aa), FASTA scores: opt: 1210, E(): 1.8e-73, (82.5% identity in 206 aa overlap); Q9F9R1|SOD from Mycobacterium paratuberculosis (207 aa), FASTA scores: opt: 1207, E(): 2.9e-73, (81.65% identity in 207 aa overlap); O86165|SODM_MYCLP|SODA|SOD from Mycobacterium lepraemurium (206 aa), FASTA scores: opt: 1204, E(): 4.5e-73, (82.05% identity in 206 aa overlap); P13367|SODM_MYCLE|SODA|ML0072 from Mycobacterium leprae (206 aa), FASTA scores: opt: 1169, E(): 9.6e-71, (80.5% identity in 205 aa overlap); etc. Contains PS00088 Manganese and iron superoxide dismutases signature. BELONGS TO THE IRON/MANGANESE SUPEROXIDE DISMUTASE FAMILY. ALTHOUGH FOUND EXTRACELLULARLY, NO SIGNAL SEQUENCE IS PRESENT. AN ALTERNATIVE SECRETORY PATHWAY MAY BE USED.; sodB; sod FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems (UniProt: P17670) CATALYTIC ACTIVITY: 2 superoxide + 2 H(+) = O(2) + H(2)O(2) (UniProt: P17670) COFACTOR: Binds 1 iron ion per subunit (UniProt: P17670) SUBUNIT: Homotetramer (UniProt: P17670) SUBCELLULAR LOCATION: Secreted protein (UniProt: P17670) SIMILARITY: Belongs to the iron/manganese superoxide dismutase family (UniProt: P17670) CAUTION: Although found extracellularly, no signal sequence is present. An alternative secretory pathway may be used (UniProt: P17670) >GeneID|886174 [Mycobacterium tuberculosis H37Rv ] 4320704..4321327 gtggccgaatacaccttgccagacctggactgggactacggagcactggaaccgcacatctcgggtcaga tcaacgagcttcaccacagcaagcaccacgccacctacgtaaagggcgccaatgacgccgtcgccaaact cgaagaggcgcgcgccaaggaagatcactcagcgatcttgctgaacgaaaagaatctagctttcaacctc gccggccacgtcaatcacaccatctggtggaagaacctgtcgcctaacggtggtgacaagcccaccggcg aactcgccgcagccatcgccgacgcgttcggttcgttcgacaagttccgtgcgcagttccacgcggccgc taccaccgtgcaggggtcgggctgggcggcactgggctgggacacactcggcaacaagctgctgatattc caggtttacgaccaccagacgaacttcccgctaggcattgttccgctgctgctgctcgacatgtgggaac acgccttctacctgcagtacaagaacgtcaaagtcgactttgccaaggcgttttggaacgtcgtgaactg ggccgatgtgcagtcacggtatgcggccgcgacctcgcagaccaaggggttgatattcggctga >gi|15610982|ref|NP_218363.1| SUPEROXIDE DISMUTASE [FE] SODA [Mycobacterium tuberculosis H37Rv ] MAEYTLPDLDWDYGALEPHISGQINELHHSKHHATYVKGANDAVAKLEEARAKEDHSAILLNEKNLAFNL AGHVNHTIWWKNLSPNGGDKPTGELAAAIADAFGSFDKFRAQFHAAATTVQGSGWAALGWDTLGNKLLIF QVYDHQTNFPLGIVPLLLLDMWEHAFYLQYKNVKVDFAKAFWNVVNWADVQSRYAAATSQTKGLIFG STAG3 Homo sapiens 10734 57863310 AC005071 NP_036579 9606 7 94410282 94446766 + stromal antigen 3 5.05 132581.25 1225 >gi|157734172:94410282-94446766 Homo sapiens chromosome 7, alternate assembly HuRef, whole genome shotgun sequence CCGCGCTTTTCTGGAATCTTTCGCCCCCGGAAGGGCAGCGGCGGGCGCCTGTGTGGAAGGTGGGGTGGCC AGAGACACCCGAGGGACCTGAGGTGGGGACCTTCCCTGAACCCTGCTCGGCCAGAAGTCACTCTTCCAGG CTCTGACAGTGAGGTTTCTCCTTGACCCAAGAATAGGCCCTCTCGCGACCCTCCCGGCCCTGACACTAGG AGCCTTCCCGGGCCGTGCCCCCAATGGCCTCCGCCACAGAGCTGGAGAATCCTCTCCCCAGGCCTCCATA GACTGAGAGTCCATTCTCTTAAGCGCTTGGAAGGCTCTTTATCCTGGAGCCGCTTACCCCAGGGGCAGTC GTAGATATTTTTACCTTCTGGAAAAATAGGAAACGCGCACCTGCCTTTCCTCCTTTTGCGTTTCCAGCCC TATTCTCATGGCGCCTCCACCCCCAAATCCTTAAGAGCCTCCTGTTCCATTCCAGGTGGAATCAGGTGCT AGTCCTCTGGAAACGAAAGCCTGGGGTCTTCTTGTATTTTTTTTCTTATTTCTTTCTTTATTTTTTTTAT TTCATTTTAAAAAACCTGTAAGTTCCCTTGCAGAGGGCGGTTTATACTTCCGAAGCCTCCAGAGAGGGGT GCGTCTGAAGCTGCCCTGCTGTCTGCCTTGCTTGGCTTTTTTTTTTTTTTCCATTTAATTTTTTTTTTTT AGAGACAGGGCGTTGCTATGTGGCCCAGGCTGATCTTGAATTCCTGGACCCAAGCGATGCTCCCGCTTCG CCCTCCCAAAGTACTGGGATTATAGGCGGGAGCACTGTGCACCACGCCTCGGCTTTTGGAGAGCAATTCC AAACCCACTGCCCAGTTTCTGGTGTCTGTAATCCCTTGGTGCTTTAGGTCCACTCTTTGCCTTATTTTAT TTATTTGTCATTACTTTTTTTTTTTTTTGAGACAGAGTCTCGCTCTGTTTCCCAGGCTGGAGTGCAGTGG TGCAGTCATAGCTCGCTGCAGCCTCGACCTCCTGGGCTCAGGCGATCGTCCCGCTTCATTCTGCCGAGTA GCTGGGACTACAGGTGCACCACCACACCTGGCTAGTTTTTTTGGTTTTGGTTTGGTTTTTTCGTAGAGAC AGGATCTTGCTATATTGCCCAGACTGATTTTGAACTCGTAGGGTTAGGCAGTCCTCCTGCTTCAGCTGCT CAAAGTGCTGGGATTCCAGGTTTGAGCCACCCTGGGCACCTTGCCTTTTTTTTTTTTTTTTTAATTATGA GTTAAATTCCCTTGCAGCAGGCAGTTTACACTTCTGAAGCCTCTAGAGAGGGCTGTGTCTGAAGCTGCCC TGGTGTGTACCTTGCTTGGGTTTTGTTTTTTTTTTTTTGGGTGTGTCTCATGTTGCCAAGGCTGGTCTCA AACTGCTGGGCTCAAGCAGTCCTCCTGTCTCAGCCTCCTGAGTAGCTGGGACCACAGGTGTGCGCAACAA CACCTAGCTAGTTTTTGCTTATTTTTTGTAGAGATGGGGTCTTGCTATGTTGCCCAGATTGGTCTTGAAC TACTGGGCTCAAGCAGTCCTCCCGCCTCAGCCGCCTAAAGTTCTGGGATTACAAGTATGATCTACCACAC CCAGTGTGCAATTTTTTTTTTTAAAGAAAATACCTATGAGTTAAATTTCCTTGCAGTAGGCAGTTTGCAC TTCCACAGCCTCTAGAGAGAGATGTGTCTGAAGCTACCCTGGTGTGTGACTTCCTCAGCTTTTGGAGAGC AACTCCAAACCTGCTGGCCAGTTTCTGGGGTGTTGTTGTGCTTAATCCCTTGATGCCTTAGGTCAGCTCT TTGTCTTATTTATTTATTTATTTTGAGATGGAGTTTCACTCTTGTTGCCCAGGCTGGAGTGCAGTGGCAC AATCTCAGCTCACCACAACCTCCGCCTTCTAGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCAAGTAGCT GGAATTACAGGCATATGCCACCACACCCGGATAATTTTGTATTTTTAGTAGAAATGGGGTTTCTCCATGT TGGTCAGGCTGGTCTCGAACCCCTGACCTCAGGTGATCTGCCTGCCTCGACCTCCCAAAGTGCTGGGATT ACAGGCATGAGTCACTGTGCCCAGCCATTTGTCTTATTTATCATTACTATTTTTTTGAGGGGGGACAGGG TCTTCTTCTGTCACCCAAGCTGGATGGAGTACAGTGGTGCGATCGTAGCTCACTGCAGCCTTGAACTCCT GGACTCAAGCAATCCTCCCACCTCAGCCTCCTGAATAGCTGAATACAGGCATGCTCTACCACACCTGACT AATGATTATTATTATTTTTTTTTTAGAAATGGGGTCTTGCTGTATTGCCCAGGCTGGTCTTGAACTCCTG GGCTCAAGCCATCCTCCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAGGCATGAGCCACGGTGCCCAGC CTTTGTCTTAGTTTTTGGTCCCCCTCACCTGACCCCACAGTTCTAGAACCATTCCCTTGGAAAGAAAGGG ACTGGTCAGGGCTGGGGATGGAGAAGTGCTGTGGTAGGAGCCCTTTCTTCTCTTTCTTCCCCAGCTGGAT CGCCATACCTACCCTGTGGTCCTCATCTTCCTGGCCTCATAGCTCCTCCTCTCCAAGCATGTCTTCCCCG TTGCAAAGAGCTGTGGGAGATACCAAGAGGGCCTTGTCTGCATCTTCTAGTTCCTCTGCCAGTCTACCCT TTGATGACAGGGACTCAAACCATACCTCAGAGGGGTAAGTAGATGTTGCATTGTGTGGCCACTTCCTGTG TCCCTGGCAGCCTTCCCCCTCGTTTTCCCACATTGATGATAATATGCGTGGGACTGTGTGAAAACACCAG AAGTGCTATACTCGTTCAGCACAGTGACACCCTCCCTCCAGCTCCAGGTGTTTTTTTTGGCGGTCCTAGA TGTTGTAGACATCAAAATAGTAGTACATCCTGTTTTTTTTTTTTTTTTTGAGACGGAGTCTCGCCCTGTC GCCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTTACTGCAACCTCCGCCTCCTGGGTTCAAGCACATCC TGTTTTTAATCCATCATCCATCTTGTCTAAACCTCTCTTAAACACATTTCTATGTTCAGCATGTACCAAC CCTGGTGGGGGCTGAGAGCTGGCAGATGGGGGAGTAAAGAGTTCTTTATTTGTTGCCTGGTAAATTGTAT ATACTTTTTGGCTAGTGTCCCCTATTTCTTCTACTTGGGGATTTTGTAAATAGGTCTGTGTATCCTTATT TATTACCATATATGAGTTTATTCTGTGTAGGTTATAATAATATATCTTGGTTTTTTACCTTTCCAGAAGG AAAAAAGCGAATTTCATACAGATCTGCTTTATCCCCTGGCTGTTTTAATTGGCTTTCTCTGAATCATTTC TGTTTCTGTCAGTGCTGCTGTGGGGGACCAGATTATATCAAAGTTATAAACTTGAATTGATAAGACATGT GGTTTATGTTTCAAGGGGGTGAAATTTAACTCTAGATACATATTACTCTCCCATTTATGGATGAATGATA GATGACTTCCTGAGCCATGATTATCTTTCTCTACAAAGAGCAGTGATCTCGCTACCTGCTAAGTGAGTAT CATTTGATTCTCTTCTTCGGAGAGAATACTAGGAAGAGTTGTCCTTCTCCTGCCTCATTTCCTCATTCTT CGTCTCCATTGCTTTCACGTGAAAGTTTTCCTTTAAGGCTTTGTCCCGGCCGGGTGTGGTGGCTCATGCC TATAATCCCAACACTTTGGGAGGCCGAGGCGGGTGGATCACCTGAAGTCAGGAGTTTGAGACCACCTTGG CCAACACGGTGAAACACCTGTAATCCCAGCTACTCGGGAGGCTGAGGTAGGAGAATCGCTTGAACCTGGG AGGCGGAGGTTGCAGTGAGCTGAGATCATGCGATTGCACTCCAGCCTGGGTGACAAGGGTGAAACTCCGT CTCAAAAAAAAAAAAAAAAAAGACTTTGTCCCAACTTCCTACTAACAATAAGCTCATTTTTTCTTAAAAT CTGGATATGTTTTTCTCATGGAGATGGGGTGCGGTGAGAGACAGAGAAACCATAATTAATTAGCCTTTTA TATGGAGGGAATAGGGTGGTTATATTTAAAGAGAACCATACTTTCTCACAGGAATGGCGACTCTTTGTTA GCTGATGAAGACACTGACTTTGAAGACAGCTTGAATCGCAATGTGAAGAAGAGAGCAGCAAAACGACCAC CGAAAACAACACCGGTGAGTCAGCCAGTTTTCTTTTGTTTTTGAATCTTGTGGGGGAAGCAGCCCAGCTA CTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGGGGCAGAGCTTGCAGTGAGCCGAGATTGCGCC CCTGCACTCTAGCCTGGGCGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAGAAAAGAAAACCTAAAA GAGGTTCAGGTGATACGGTTCATAAAAGGACAAGATTTACAAAGTATTAAGGGATGGAAATAGGAAGAAG AGAAATTTTGAAGAGAAAAGAAGAGAAAAGGACGTTAGGATGTAGAGATACAGGAAGGGGACTCAAGGAC TTTTTTTTTAAGGGAAAAAAATAAACTTCATAACAATATAAAAATCAATTATTTCAGGACTGAGTCTAAC TCAGACTCTGCTGTGGAATTGGCAGCTTAAGCCTACTTGTGTGATAATGATGAAACCAAGCGTTAATGTC ACTGTTACCTTTTTTGTTTTTTTTTCATATTTCTGATCTTTTTATACATATTAGGTGGCAAAACATCCAA AGAAAGGGTCCCGAGTGGTACATCGTCATAGCCGGAAACAGTCAGAGCCACCAGCCAATGATCTTTTCAA TGCTGTGAAAGCCGCCAAAAGTGACATGCAGGTAAAGCAGTGTCTCACCTCTGTTGATACCATCTCACTT TTTGTAAGGTGGTAAGGACAAGTGTCTACAAACTTGATTTGACGTGAACATTTTAGTGAGTGTAACATGT CAAGAAAGATAAGGTTATGGGGTGAATCCTTGGAGTGATAGAAGGGTAACCTTGAATGAGAGAGCATGTT CTTTTTTTTTTTTTAGACAGAGTCTCTTTCTGTCGCCCAGGCTGGAGTGCAGTGGCACAGTCTTGGCTCA CTGCAGCCTCTGCCTCCTGGGTTCAAGCGATTCTTGTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGC GTGTGCCACCACGCCCAGCTACTTTTTGTATTTTTAGTAGAGATGGGGTTTCGCCATGTTGGCCAGGCTG GTCTCCAACTTCTGACCTCAGGTGATCCGCCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAG TCACTGCACCTGGCTTTGAGAGAGCATGTTCTTTTCTTAAAGGTATCACAGGAGTGATTATGCCTTTATT GATTGATGGAGCAGATGAGGAGAGGCATTGCATGACACAGGAAGGAAGCTTGGATGTGGGTGGCACCTTG TTCACCCATGTTATTTTTTAGCTCCATCTTGACAATTATACTTAATTATTTTTTCCTTGTTTCTTTTTTT ATAATTTTATTGAAGTTCTAATACCCGAAAGTGTACAAAGCTTATATGTAGTTTAAAAAATAAAAATGAA ATGATTACTCAGATCTCTAGCCCCAGCTCAAGAAATGAAACATTAATAGTGCTGTAGAGATCCTTTGTGT ACCTTCTTTCTACTCCATTTCACAGTCTCCTTATTACCCACAGGTAAACACTAGAGTGAATTTTGTGTTA ATCTTTCTCATCTCTCATTATAGTGTTACCACTATGAATATGTTCTTTAATTATGTAAATGAATCCATTA TTCAGGCTTCTATGATTTAATTATTTGAGTTAACATTGTTTGAGATTTACCCACATTAATGTGTAATTTT ATAGTTTCTGGGAAAATATTTAAAATTTTCAAACATATAGAATTTTGCTAGTTATATTTTCGTTATTTGA TTTCTAACTTCATTGTGTTGTAGTCAGGGAATGTGGTGTTTGAAATTTATGTAGGGGCCGGGCACAGTGG CTCACACCTGTGATCCCAGCACTTGGGGAGGCTGAGGCGGGCAGATCACTCGAGGCCAGGAGTTTGAGAC CAGCCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGCATGTGGTGGCGTTT GTCTGTAATCCCAGCCACTTTGGAGGCTGAGGCATGAGAATTACTTGAACTCGGGAGGCGGAGGTTGCAG TGAGCTGAGATTGTGCCACGGCACTCCAGCCTGGATGACAGAGTGAGACTCTGTCTCAAAAAAAAAAAAA GAAAGAAATTTATGGAGACTTAGCCTTATGGCCTAGTATTGAGTTGGTACATTCGTTTTATCTTTTCTGT TTCTTTTTCCACCTTTGCTTGCCTTCCTTTGGATTTCTTATCTTCTGCAAGGACCTGAGCACATTTTAAT TCCAAATGTCTTTTCCACCTTATATGCAGTTGTACAGCGTGTTAGGTTTTTTTTTTTTTTTTTTTTTTTT TTGAGACGGAGTCTTGCTCTGTCGCCAGGCTGGAGTGCAGTGACGTGATCTTGGCTCACTGCAACCTCCA CCTCCCGGGTTCACATGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGATGCATGCCACCAC GCCCAACTAATTTTTGTATTTTTAGCAGAGATGGGGTTTCACCGTGTTGGCCGGGATGGTCTCGATCTTT TGACCTCGTGATCCGCCGGCCTCGGCCTTCCAAAGTGCTGGGATTACAGGTGTGAGCCACCATGCCTGGC CTTTTATTTCTTTTTTTTAGCCCATTCTCCCCCTTTTATTTTTAAAATTTTATTAAACATAGTCATTTTA TAATCTATGATAAATTATTATCTAAAGTCTTTGTGAGTATAATTCTGTCAGTGCCTTGTTTCTTTATATG TTTTACTAGGTTTTATTGTGAGCCACTCATTTTCCCTGGAGTTCTTATGTGTTGGAATTCTTTGGGGCCT AGGCCAAAGCTGGGTTTTTAAGAAAGGAACCTGCTTTTGCTTTGACAGTTGTCTGGGGACACTCCAGAAT CACTTTTTTTTTTTTAAGACAGGGTCTCATTCTTTCTAGGCTGGAGTGCAGTGGTGCTATCACGGCTCCC TGCTGGTTTGACCTCGCTCAGTGGCGAGGTGGTGATCTTCCCACCTCAGCCCCTCTCATAGCTGGGACTA TAGGCATGTGCCACCATGCCTGACTAATTATTCTGTTTTTGGTAGACCAGCCTGGGCAACATGAGTTTCA CTTTACTTGGGTTACTGCTCCCAGCCTGAATCGCTTTTAATTATCTGAATGATTATTCTGGGCTGGGTGT GGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTAAGGTGGATCTCACTTGAGGTCAGGAGTTCGA GATCAGCCTGGCCAATATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCATTAGCCAGGCGT GGTGGCGCACACCTGTAATCCCAGCTACTTGTGAGGCTGAAGCAGGAGAATTGCTTGAACGTGGGAGGTG GAGGTTGTAGTGAGCCGAGATCACACCATTGCACTCCAGCTTGGGCTACAGAGTGAGACTCCATCTCAAA AAAAAAAAAAAAGGAAAAGAAAAAAAAAGATTATTCTGATTATTCTGATCATGTATGTGGTGTGAATCTT GATTGCAAATCCAGATCTGAGGGCTCACTATGGTTATGAATTCTCAAGAAAGAATTATTTTTTTTCCCTC CACTGTACCCTCAGGTCATAACAAGCTAGTTTTCTTGCTGTCTCTATCTGCCCGGTAGGTTTAGTGTTCA ATCTGACTTCCTTGAGGATCTGACTCTTTGCAGGACCCAGCTTTATTTAGGTGCCTCCTCTTGGACTACT CGTCTTGGAAAAAGCTTAGGCTCTACCTCCTGTGCTCTGCATTCCATGCAGCCATCTGGATACAAAGTCA TAAATCCCCAGGGATTAATGAAACCTTTAGGGCAAAAGTCAGTTTGTTTACCTCCCAGGGTTGCTACTTA CACCTCATTTTTGGCCTATATGAAATTTTACCTTTCATTCTAGTTCACCAGTACATTGTGAAAGATGTTT TAAATGTTTTACAGAAGTTTAGATGGTTCAACTGGGAAGATCATATAGGATTTCTATTCTGTCATATTGC CAGAAACAGAAGTCATCTACATTTTTTTTCCCTAGTCTTTGGTAGATGAGTGGCTGGATAGCTACAAGCA AGACCAGGATGCAGGATTTCTGGAGCTTGTTAACTTTTTCATCCAATCTTGCGGATGTAAAGGTGAGGAA ACTGCTCCCCCTTTCTAATTCCCAGCCTTTTGTTCCTATGTTATGAAATTCTTTCTCCATTTAGATAAGT AGGATTAGACATTTCCTAAATAAAGGAAGATGGTAAAGCATTAGAAAAATTTTACTTATTTAGGCCGGGC ACTGTGACTCACACCTGTAATCCCAGCACTTTGGGAAGCTGAGGCAGGTGGATCACTTGAGGTCAGGAGT TTAAAACCAGCTTGGGAAACATGGCGAAACCCCTACTAAAATACAAAAAATTATCTGGGTATGGTGGCTC ATGCCTGTAGAATTGCTTGAACCCAGGAGGTGGAGGTTATAGTGAGCCAGGATAGCGCCACTGCACTCCA GCCTGGGCGACAGAGCCAGACTGTCTTCAAAAAAAAAGAGAGAAAAGTTTCACTTCTTTAACTAAATTTT TTATTGATAAATATGGGAAGTGATCCAGTAGGGAACCATGTTGGTTTAGAGGCAAGACACTTTAGGGAAA AAGAAGTAATCACGCTTATGTTATGGCACAAATTTGAAGACACCCTTTGGTCTTTCTGGATGCAATAATG TTACTCTTTGTTTCCCAGCTACTTTGCCCATATTTCTCCTCTGTAGCTGTGGTACCCCCAGAACTCTTAT TGCCATGGCTTCGTTTATTGGGACAGATGGTCTCAGTCAGGTTGTATTATATAAGAAATCAGCATCTGGC TTAGTTTTATTGCTGTCTTTGTTTTGTTTTTTTTTGTTTTTTTTTGAGACAGAGTCTCACTCTGTTGCCC AGGCTATAGTGCAGTGGCATGATCTCGGCTCACTGCAACCTCTACATCCTGGATTTAAGCGATTCTCGTG CCTCAGCCTCCCAGGTGGCTGGGATTACAGGCGAGTGCCACCATACGTGGCTAATTTTTGTATTTTTAGT AGAGATGGGGTTTCACCAGGCTGATCTTGAACTCCTGACCTCAGGCGATCCCCCTGCCTCGACCTCCCAA AGTGTTGGTATTACAGGCATGAGCCATCGCGCCTGGCCTGTTTTTTGGTTTGTTTTTTTTTTGTCGTTGT TGTTGTTGTTTTTTGGCAGTTTTTCACTGCTTGATTCCTTTTTTTTTTTTTGAAACAAATCCTGCCTCAG GTTTATTTGTACAAATAGTGCAAGGGGACACCAGTCCTGACCTCATGCTGGCAGAGAGAGATCTCTACTC TGAAGCCTTTGTACAGGCCTGGGTACTTTTGGGAGCCTGAGCTGGAACTGAAGGTGGAGGTGCAGCCTGG CCCTTCATTTGATCCTTGGCCTTTGGCTGGCACAGCCTGAGCCCCTTGGCATTGTGGGCACAAGCATGCT TCTCAAGCTTGGGGTGGGCAGTGTAGGCAAGTCGGCTTAACCTCCTTGGGCTTTATGAGGGACTTGATAG CCTCAGCATGTGCACTCATGGCCCTATGGCCCTGGCATTGTTGCCCTGCATCATCTTTTTTTTTTTTTTT TTTGAGACAGTCTCACTTGTTGCCCAGGCTGGAGTGCAGTAGTGCGATCTCAGCTTACTGCAACCTCCGC CTCCCAGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACCCGCCACTATG CCCAGCTCATTTTTTGCATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAATTCC TGACCTTGTGATTCACCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGACCGACTGCACCCGGC TGGCCTGCATCTTCTTTAGGCCGTTCTTGTTGTGCTTCTTGGCAAAGCACATATTCCTCAGGAATTTGGG TCTATCCGCTTAAGAGATTTGTATCTTTGTGATTGGGGTTTCTTGATGCCATTTCTGTGCAGTTTTCAGC CTGGTTGTGTGAGGTGTGGTTCTTGGATTTGGCCATGTCTACACCATAACCTGTGGCTCCCTTCCTTTGG GTTTTTAGAGAGATTGCCTTATAGCAGATACATGGTTGGATGTTGGCAGACTGTTGACCAGGGGGTCTTC TCATTCCCCACCTAAGCTCTTTGCAGAATGTTTCCTTGCTCATCTTCAGGTATATCTGTAAATGATCAAA GCATCCTGTTATTTTCCTTGTAAGCACCTCATATCCTTCATTCTTTAGGCATTGTGACCCCTGAGATGTT CAAGAAGATGTCCAACTCAGAGATCATCCAGCACCTAACAGAGCAGTTTAATGAGGTGGAAGAAGATGAC CAGGATCCTCTTACCCCTCTTATTTCAAAACACAATGCCTATTATCCCCTTCTTTTTGATTCTGTGAAAT AGGGTAGCTTTTAGAAGGAGGAATTCAGTCTCTTGGGGGTAAAAGAGATCTGAATATGCCTCTCAAATTG GGGAGAATACCAGGAAATGAGGTATAACAGATATTTTTACTGGACTGTTGAGTTTTGACATCCAAGCCCC TATGACTTCATGGACCTGGTAATAACTTTCCCATCCTTTTCATACATCCTTTTGTAGGACTCGGGGGACT ACCCTCTCATAGCTCCAGGTCCATCCTGGAAGAAGTTCCAGGGCAGCTTCTGTGAATTTGTGAGGACATT GGTCTGTCAGTGCCAGTACAGCCTCCTCTATGATGGCTTCCCTATGGACGACCTCATCTCCCTGCTCACT GGCCTCTCAGACTCACAAGTCCGCGCCTTCCGTCACACTAGCACCCTGGCTGGTGAGCATTCATTTTTAC TCTGGACATTCTCCTGGGGATTTATAGGACTTTCCTCTGTTCTCTGATTCAGGATCTTCTTTCCTACCTG CATCTTGGCTCTTCACTTCAAGGCCATGCCTCTTTTATCCTAAACTTCAATCCAGTTTCTCACTAGTGGA GTTTTGAGAAGGGGTAGATCACAGCAACAATTTCCAGGTTTAATATTTTTGTCAGAGCTCACCACTAGGG GTATGCTGATGGTTAAAAGAAGACCACAAGGGTTCGAGGGAAACAGTCCACTCCAGGACAGGCCGTCTTA GGATTTTAGGGTCATCCTGCTCTGACTTCTCATTTTCATTGCCCTTCTTTCCTTCTATTTTTTCCTTCTA CTCATCCTCTCTCCTCTGACCTCAGTAATGATTTCTTTATCTCTTTTTCCTTTCTCAAAGCTATGAAACT GATGACCTCCCTGGTAAAAGTTGCCCTCCAACTGAGTGTGCACCAAGATAACAATCAGCGTCAGTATGAG GCTGAAAGAAACAAGGGGCCAGGGCAGAGGGCACCTGAGCGGCTGGAGAGCCTGTTGGAGAAACGCAAAG AGGTGAGGAGTGTTCCCTGCTTCTTCCTTCCCTTTTTCCCAACTTGCACCCACTTCTGCCACAGACCCCC TTATCAGGCCCTGGGCAGTCTTTCAAGATCATGAATAATGGAGTCTGGCAATAGTTTCATAGACCCATCC TGTAAGGGGTCATTACATGCTCATCTTGTTTCTGGGTTGTGGGCTCAACAAATAATGTGTAATTTAGAGT TCTCTCTAATTCCACTTTTGCTCAGTTTTTTTTTTTTTGTCAGCACTCATTGTTAGGAGTGTTTAAGTGC TGTACCTGCCTGTCATGCAGGGAATTAAGTTTTGACCTACTTGTCAAGTGTGCAGTGTGCAAGTGAGTGC AGGAGTACTAAAAGCCAACCATTGACAAGGGAGGCCAAATTTTCAAGGAATGGAAAGAAAACAGGCCTCC CAGTGAACTCGCTTTAAATTCTGTTGAATTTAGTCACTAGCTCCATAGCCTTATGTAACCTCTCTTCAGT TTTCTTTTCTTTAAAAAAAGGAATAATCTCACCTTTTTGTGAAGAATAAATGAGATGAGTAAATTGCCTC GCTTGGCTCATAGTAGATACTTGTTTTGTTTCCTTTTAAACTTAAAGATGACCACTGGGGGGCATAAGCC ATAGCAAAATCTTTTACGCTGATGAAATCTTTAATCAGCTGGGTCTGTCATAAGAGTAACACTACTGGTG TTTTCTCCTCACTCTCTGTTAGTGTCTAAGACCCCTCCTATGAGGTTGGCAGGACTCACCATGTCTGACC ATATCATTTGGCTGTTTCTCTAGCCCTCACAAAAGATCTCTTCCATTCTAAATCACTAGAGAAGGAAACT TTCCACTTCCCTTATTCTCTTTTGTTATTTTGCATTGTCAGGTTGATCTTAAAAGCTTGAATATTAATCT ATCCCGGATTTTAAATTATGTACACTAGTGCGAATCTTTTCTTTTCTTTTTCTTTTTTGGCTTCTCTGGG CTCTTGCTTAATCAATTATCATTATAATTCCCCCATTTTATCCTAAATTTCTTCCTCCCCACTATTTTCT TCTCTCCACTTATAAATGTATATAGGCTCTTGAATCTTTATTTCACTCTGCTCCTTTGTGAGTTACTCTT TTTTCCATCCTTCCTTTTAGGATAGTATTTCCTTACTTCCTTAATGTCCAACTAACTGAATCCTTTTTGC TACCTAGCTTATTTCCCCATTTTCTTTGAAACTGTTCTTTCTAAACCAATGACTTCCTAATTCCTAAAGC CAGTAGCCTTTTCTCAGTCTCCCTTTCCCAAATATTTTACATATTTGCTTTACCTATTTTTGCTATTTTA GTGTAAAATATGGACATATAGGTACTTCAGCCCTAAAGACTTAAGTATGTCCTGTCGAAAATGATATGTT GTTCTTACATAATTGAAGAGGTGGGGCCTGTTGTCTTAAAGGATGTTCCCTTTCTCCTTATAATTCTGTT TCCTTGGTGTCTATTGTTCATGTGATCCAGGATTTTTCAATAATGGCACTATGGACGTTGCGGGGCTGTC CTGCCATGGTAGGATGTTTAGCAGATCTCTGGCCTTTATCTACTAGATAGATACCAGTAGTACCCTGCTC CCCCAGCAAGTTGTGGCAGCCAAAAAAATCTTTAGACACTGCTAAATCTCCCCTGGGGAACAAAATTAGC CTCAGTTGAGAACCACTGATCTAATTCCTTTCTTCTCATTTTTGCTAGTTCCACTTCTATTAAACTGCAA CTTACTAAAAATTTGTTTTCCAAATTGTTAGAAACATATACGGGCATCATACCTCAAAGATATTGCAGAT TCAATTCCAGACCACCATAATAAAGTGAATATTGAAGTACAGTGAGTCACACAATCAAAAAATCAAAATT TTTAATTCTCAGTGCATATGAAAGTTGTGTTTACACTGTAGTCTGTTAAGTGTGCAAGACTATTGTCTAA AAAGCAGTGTATGTACCTTAATTTAGAAATATTTTATTGCTAAAAAATGCTAATGACAATCTGAACCTTT AGCGAGTTAAATCTTTTTGTTGGTGGAGGGTCTTGCCTCCATGTTGATGGCTGCTGACTGATCAGGGTAG TGGTTGCTGAAGGTTAGGGTGGCTGTAGCAATTTCTTGAAATAAGACAACAGTGAAGTTTGCTGCATCTA CTGACTCTTCCTTTTACAAAAAATTTCTCTGTAACATGAAATGCTGTTTGATGACATTTTACCCACAGTA GAAATTCTTTCAAAACAAGAGTCAATTCTCTCAAGCCCTGGTGCTGCTTTATCAGCTAAATTTAGGTAAT AGTCTGAATCATTTGTTGTCATTTCAACAGTATTCACAACCTCTTCACCAGGAATAGATTCCATTTCAGG AAACCACTTTCCTTGCTCATTCATAAGAAGCAATTCCTTATTTGTTCAAGGTCATGAAATTGCAGCAATT CAGCCACATCTTCAGTCTCTACTTCTAGTTCTCTTGCTATTTCCACTGCACGTGTAGTTTACTTCCTCTA CTGAAGTCTTAAACCTCTCAAAGTCATCCGTGAGGGTTAGAATCAACTTAACTCTTGTTAATGTTGATAT TTTGACCTCTTCCCATGAACCACAAATGTTCTTTATGGCATTTAAAATGGTGAATCCCGGCCAGGCACAG TGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGACTACGTGAGGTGAGGAGTTTGA AACCAGCCTGGCCAACATGGCAAAAACCCATCTCTACTAAAAATACAAAAATTAGCTAGGTGTGGTGGTG CTGCTTGTAATCCCAGCTACTTGGGAGGCTGAAGCAGGAGAATTGCTTGAACCCAGGAGGCAGAGGTTGC AGTGAGCCAAGATTGCGCCACCACACTCTAGCCTGTGCTATGGAGCAAGACTCCATCTCAAAAATAAATA AAATAAAATAAGATAAAATGGTGAATCCTTCCCGGACGGGGGGTTGAGTTTTTCTGGTTTTGTTTTTTGT TTTGAAGACAGGGTCTCGCTCTGTTGCCTAGACCGGAATGTACTGGTGCAGTCATGGCTCACTGCAGCCC CAACCTCCTGTGCTCAAGTGATGTTCCTGCCTTATCCTTCCAAGTATCTGGTACTACAGATTGCATACCA CATCTGGCTAGTTTTTGTTTTGTTTCTGTAGAGACAGGGTCTCACTGTGTTGCCCAGGCTAGTCTTGAAC TCCTGGGCTTAATCAGTCCTCCCACCTCAGCCTCCCAAAAAGTTGGGATAGTAGGCATGAGCCACCATGC CTAACCCCAGGAGGTTTTAAATGGACTTTGCCCAAATCATCAGAGGAATCACTGTGGCAGCTATAGCCTT ATGAAATGTATTTCTGAAATAATAAGACTTGAAGTTGAAATTACTCCTTGTTTCATGGGCTGCAAAATGG ATGTTGTGTTAGTGGGCATGAAAAAAGCAGCATGGATCTCCTTGTACATCTTCACCAGAGCTCTTGGGTG ACCAGGTGCATTGTCATTGAGCGGTAATATTTTGAAAGGAATCTTTTTTTCTGAGCAGTAGGCCTCACCC ATGTGCTTAAAATATTCAGGAAACCATACTGTGAACAGATGTGATATCAGTTAGGCTTTGTTGTTTCATT TATAGAGCATAGGAAGAGTAGATTTAGCATAATTCTTAAGGGCCTTAGGATTTTTAAAATGGTAAATGAG TATTGGCTTCAACTTAAAGTCAGCAACTGCATTAGCCCCTAACGAGGAGTCAGGCTGTCCGTTGAAGCTT TGAAGCCAGGCATTGACTTCTCCTCAATAGCTGCGAAAATCCTAGATTTTCCAATATAAGGCGTTTTCCA ATTATAAGGCAGTTTAGTCCACATTGAAAATTCATTGTTTAGTGTAGCCACCTTCATCAGTGATCTTAGC TAGATTTTCTTGATAACTTGTTGCTTCTCTATCAGTATTTGCTGCTTCACCGTGTACTTTTATGTTATGG AGAGGGCTTCTTTCCTTAAACCTCACGAACTAACTTCTAGCTTCAAACTTTTCTTCTGTGGCTTCCTTAC TTTGCTCAGCCTTCATAGAATGGAAAACAGCTAGGACATTGATCTGGATTGGGCTTTGGCCAAAGGGAAT GTTGTGGCTGGTTTGATCTTCTGTTCGGACTACTAAAACTGTCTCTGTATCAGCAATAAGACTGTTTAAC TTTCTTACCCTTTGTGTGTTCACTGGAGTAGCATTTTTAATTTCTTTCAAGAATTTTTCTTTTGCATTCA TAACTTGGCTGTTTGGCACAAGAGGCCTAGCTATCGATTGGCCTGTCTTGGCTTTCAACTAAGCTTAATC ATTTCTTTTTTTTTTTTTTTTTTTTCTTTTTCAGACGGAGTCTCACTTTGTTGCCCAGGCTAGAGTGCGA TGGCGTGATCTTGGCTCACCACAACCTCCATCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGA GTAGGTGGGGTTATAGGTGCCCGCCACCGCACCCAGCTAATTTTCTGTTTTTAGTAGAGACGGGGTTTCG CCATGTTGGTCAGGCTGGTCTCGAACTGACCTCAAGTGATTTGCCCACCTTGGCCACCCAAAGTGCTGGG ATTATAGGTGTGAGCCACTGCACCTGGCCTCTAAGCTTAATCATTTCTAGCTTTTGATTTAAAGTGAGAA ACATGTGACTCTTCCTTTCATTTGGACACTTAAAAGGGTTATTAATTGACCTAATTACAATATTTTTGTG TCTCAAGGAATGAATAGGGAGGCCTGAGGAGAGAGAGAGAGACAGAGAAACTCTTGTTGGTAGAGTAGTC AGAACACAGACATTGTTAAGTTTGCCATCTTATATGGGCACGGTTTGTGGTACCCCAAAACAGTTACAGT AGTAACATTAGGCTGGGTGTGATGGCCCATGCCTGTAATCCCAGCACTTTGGGAGAGTGAGGTGGAAGGA TCGCTTGAGCCCAGGAGTTCAAGACCAGCCTGGGCAAACATAGGGAGAGCCCGTCTCTACAAAAAAAAAA ATTTTTTTTAACTAGCTGGGTGTGGCAGCATGCATCTGGACTCCCAGCTAGTTGGGAGGCTGAGGCAGGA GGATTGCTCGAGTCCAGGAGGTGGAGGCTGCCGTGAGCCATGGTCACACCACTGTACTCCAGCCTGGGTG CCAGAGTGAGACCCTGTCTCATAAAAAAATATGTAACATTAAAGATTACTGATCACAGATAACCATAACA GATACCATAATGAAAAGGTTTGAAATATTGTGAGAATTACCAAAATGTGCCACAGGGACACAAAGTGAGG ATATACTATTAGAAAAATGGCACTGATAGGTATGCTCAATGCAGGGTTGTCACACACCTTTAGTTTGTTT GAAAAAAATCCACAATGTCTGCAAATCAATAAAGTGCAATAAAGCGAGATACACCTGTATAATGCCAGCT AGGGCTTCTGAATAGTCCGAATAACCACATGCAGAGGGGTTATTATTTTTACTAAACACAAACCTCTGAG GCATTAGTTGACTATACAGCTTCTTCCAGAATTAGAAGGTTTAAAAGAGAGCATAGGATTACGGGGGTTC ACACTATCCTATAATAAACAGGAAGTAGGTGGAAGTTGTAGTTTTTCTGTATCTTCAGGGCCTTATGCTT GTTAGGGTACAAGAAAGATTAACCCGTTTCTCCCTGTCCTCCAGCTCCAAGAGCATCAAGAGGAGATTGA GGGGATGATGAATGCCCTCTTCAGGGGTGTCTTTGTTCATCGGTACAGGTGAGCTAATGGGCCTCTCTCA TTGAAGCAGCTGGCCTTTGGTTATGGAATCTGAGGAAGTGAATAAGGTTTTCCCCCCTCCAACCCTTAGT TATTTGGTTAGTCCCAGAATTAATGCAGCTGGAGCAAAAATTCTTGACAACAAATGTTTCAAATTGGTGA GGTTTAGTTTATCACCACCTACAAAGGATCTTGTAAGTGCTTCTGGGAGAAAATAAGAGAATAAAGCATT GCTGAGTATTTTCCTGCTCTGGCTTTGCCCCAGATCTGCCCCAACTCCTCATCTTTCTGGGGTTGGAGAC TTGAGGATAAGAGCAGAGAATAGAAAGGCGGGGCTTTTCTGAGGGATGCCTGCTGCCTGGTGAGCCTTCT CCTGGCCCAGCCTGCTGTCTCACTGTGCGAAGGGCAGGGGAGTTTTATACTGTTTAATGACCTGATCAAA TTAAGTTAAAAAGAATATATGTAGGCCGGGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC CAAGGCAGGCAGATCACCTGAGGTCAGGAGTTCACAACCAGCCTGACCAACATGGAGAAACTCCATCTCT ACTGAAAATACAAAATTAGCCGGGTGTGGTGGCGCATCCCTGTAATCCCAGCTGCTCAGGAGGCTGAGGC AGGAGAATCGCTTGAACCCAGGAGGTGGAGGTTGCGGTGAGCTGAGATGACGCCATTGCACTCCAGCCTA GGCAACAAGAGTGAAACTCCATCTCAAAAAAAGAAAAAAAAGAAAAAAAAAATTGTAGATGTTTCTCACT CTTACCTAGGTTTCACTTCTGCCTCTCCAGAGCCTTTCAGCTGATGTAACCTTTTCTTAATGTGGAATTA AAACACACTCACACACTTTGGCCAACAGTTGAATTAACTTTTTTTTTTTTGGCAGGGACGGAGTCTCACT GTGTTGCCCAGGCTGGAGTGCAGTGGTGCGATCTCGGCTCACGGCAAGCCCCACCCCCCGGGTTTAGGCG ATTCTTCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCGCCCACCACCATGCTTGGCTAATTTTTGG CCACCATGCCCAGCCTGAATGAACTTTCAAAATTGGTTTTTAAAAGAGGTATGGAGACCGGGTGCAGTGG CTCACACCTGTAACCCCAGCACTTTGGGAGGCCAAGACAGGCAGATCACTTGAGGTCAGGAGTTCGAGAC CAGCCTGGCCAACATGGCAAAACCCCGTCTCTACTAAAAATACAAAAAGGAAGCTGGGCGTGGTGGTGCA CCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCACTTGAACCCGAGAGGTAGAGGTTGCAGT GAGCTGAGATTGTGCCGCTGCACTCCAGCCTGGGCAACAGGTGGAGTGGAGAATTAGGCTGTACAAGGCT GGTGTTTAACTATTTTATGGTTTGGTAACAGAAAGAAAATATTTACTAACTGGGTATGGCACATTTTTGT AGTCCTAGCTACTTGAGAGGCCAAAGTGGGAAGATGACTTGAACCCAGGAGTTCAAGGCCAGCTTGGGCA ACATAGCAAGAGATCCTATCCCTTAAAAAAAATATTTACATGTATTTTACTATATATATAGTATTTACAT CGGGAGGAAGAAATGAAAGGCATGGGGTACATGGCATTTCAAGAAAATAAATTCTTCCATGAGAGGGAGT TATCTGGTAGGATGGAAAGTGAGTTTGGAGAGAGGGTGGCTCTAAGAGTCAACTTATCTGTCTGTGTCTA GGGATGTCCTTCCTGAGATCCGTGCTATCTGCATTGAGGAAATTGGGTGTTGGATGCAAAGCTACAGCAC GTCTTTCCTCACCGACAGCTATTTAAAATATATTGGTTGGACTCTGCATGATAAGGTGGGATTCGAGTCA GTTTCCCTTTCCGTTTCCTTCATCTTTTTCCTGTCCTAGGACATTTCACCTTTTCCTGGGCCTGCCACTG AGATATTTTACCTAGTGACCAATGATGAACCCATCAAGGCGCTGAACCTTGAAATTCCCCTAGCACTGGG GAGAGTAGGATAAGGTAGAGAGAGACATACCCAGGAGATGAAATGACTCAAAACCTGAGGTACGGGGAGA GAGACTTTCCCATGGAAGAGAGTTATTGATGGATGGTAATGCAGGTGCTAAGGGGCAGCCAAAGGATCCT TCTCATCATGAGTACTGCTGTGATCCTTTTGTTTTTTAAGTAAGAAGACAGCTGTTCATTGTTTCTGACA TCTTAAGAGTCCTCAGTAGAGGGGACACCCAAGCTAGAATGAGGGATCGGAGAGGGGAGTCTGGAGGCTT AGTAGGGGCGAGTAGAGTGTGGTTAGTCTTATTTCCATTCTCCTGGTTTTCCCTCCTCACCAGCACCGAG AAGTCCGCCTGAAGTGTGTGAAGGCCCTGAAAGGGCTGTACGGTAACCGGGACCTGACCACACGCCTGGA GCTCTTCACCAGCCGCTTCAAGGTAAAATGGGTGGTGCTCCATGGCTTGGCTCTTTGTCGTGGTTCCTAT TTCCCCACCTTGTATCTTTCTACCCCTCATGCTCTAAGATGTCTTGGCTATCCCAGCATCTGCCTCTACG TAGGCACTCTCTTTAGGAGTCTCTGATTCTAAGAGAACAGAATGTTTGGAATGGGTGTAATGAGATATTG AGTGACTTTCTCCTTTCTGCAGGACCGGATGGTTTCCATGGTCATGGACAGAGAGTATGATGTGGCAGTG GAGGCTGTCAGATTACTGATACTTATCCTTAAGTGAGTCCTGGGAAGAGGGGAGGCCAGTGTCTCAGACC TTTGCCCTTCCAGGGTCATCTCCCTCCACCTGTCATAGCTGACTCTTCCATCTGTGCAGGTTGACTGAGG TCATTCCTGAGTTGCAGTATGTTGAGGGGGTAATATTTCTGTCTTCTCTAACTCCCCATACTCCTTTGTC TTCCGCTCTTCATTTAGAAGTTTTTTGTGAGTTATGTCCTTGTTGCTTTTGCCTCTTTTTGTTTCTAGCC TTGGTTGTGCCAGAAGACAATGTCCCTATTCACACACTCTTTCTGCTTTTCTGTGGGCAGGAACATGGAA GGGGTGCTGACGGACGCGGATTGTGAGAGCGTCTACCCCGTTGTGTATGCCTCTCATCGAGGCCTGGCCT CTGCCGCAGGCGAATTTCTGTACTGGAAGTGAGTGGGGCTCCTTTTATGTTTCTTTAACACCACGCTCTC GGTTTCTCTCCTCCATCTGCTGCTGCCCTACCTAAGGCTTCCCCATCTCTGTGAAAGTCTCCCTGGTGTC TCCTTTCTTCTCGCAGCTCCTTGGGGCTTTCCTTAGCTCTCACCTCCTTTTCCTTTATCATCTCCTTGGG CCATCTCCTCCCTCTGTGGTCGTTACACCTCCTTGTTTCCTGTTGTGCTGAGCCCTTTCCTCGTGTCCAT TCCACCAGACTCTTCTACCCTGAGTGCGAGATAAGAATGATGGGTGGAAGAGAGCAACGCCAGAGCCCAG GCGCCCAGAGGACTTTCTTCCAGCTTCTGCTGTCCTTCTTTGTGGAGAGCGAGGTGACATACACAGAGAG AAGTCTGGCTGTTGTGCATAGGACCTACAAGTGGGCTGGGGTTGGTGGCTCACGCCTGTAAGCCCAGCAC TTTGGGAGGCTGAGGTGGGAGGATCCTTTGAGCCCAGGAGTTTGAGACCAGCCTGGGCAACACAGTGAGA CCCTGTCTCTATCGAAAAAAAAAAAAAGACAAATGGAGAAGGATGGGAGTGGACACTGTAGGAAGGAGAG CGATATTTAATCAGTAACCACTTCGTAGGGTATTTTTAACATGCTATCATTAACTCCCCATGCACGTTCT CCCCTAGCTCCATGACCACGCTGCTTACTTAGTAGACAGTCTGTGGGACTGTGCAGGGGCTCGGCTGAAG GACTGGGAGGGTCTGACAAGCCTGCTGCTGGAGAAGGACCAGAGTACGTGTCACACGGAGCCAGGGACAG GGACCTTCCACCCGCTAGGGTGAAACTGGGAGGGATTGCTTGCTTCACTTGTACAAGGCAGCAACGGTGG CATCGGGTGGGGGGAGCTTGGAGTTGGAAGGTGGCTAATCTTTGATTCTATGTTTTTGATCCTCCTGGCA CTCCAGACCTGGGTGATGTGCAGGAGAGCACACTGATAGAAATCCTTGTGTCCAGTGCCCGGCAAGCTTC AGAGGGGCACCCGCCTGTGGGCCGGGTCACTGGGAGGAAGGTATGGTGTGAGGGTAGAGTGGGTAGGTCA GCAATACTCAGTCTTGACAGGCAATGTGCATCCTTATCCCCCAGGCTGGGGTTAGGGTGCTCCTTCTACC CTTTAAAGAATTTCATTTCCAGAAAAAAGGGCCAGGCGCAGTGGCTTACGCCTATAATCCCAGCACTTTG GGAGGCTGAGGCAGGCAGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTGACACAGTGAAACCCTGT CTCTACTAAAAATACAAAAAAAAAAAAAAAAAAATTAGCTGGGCGTGGTAGCGGGCACCTGTAGTCCCAG CTACTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACCTGGGAGGTGGAGCTTGCAGTGAGCCAAGATCAC GCCACTGCACTCCAGCCTGGGTGACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAAAAAAAGGAGTTTC ATTTCCAGAAAAAAGAAATCTCGTGGGAGCTACTGAGTTCTCTTTTCCTTCTAGGCAGATAAGGTCATGG GGAGGAGGGCTGCACACACCCCCTGCACCAGTGTTTCTTTACACCTCCCCCACCCCCAAGTGACTCTCAT TCCAGGGCTTAACCTCTAAGGAGCGCAAGACCCAAGCCGATGACAGGGTGAAGTTGACTGAGCACCTCAT CCCCCTGCTGCCCCAGCTCCTGGCCAAGGTACCGCTGCCCCTCCACTCTGCATCACACCAAGAGAAGTGA AAGGAAATGGCCTCTGTGGGGGTGGGAGTAGGAATTAGCAATGTGTCAGCTTGTAAGGAGGCCTCCCTGT CAATCATCACAGTTCTCAGCTGATGCAGAGAAGGTCACTCCCCTGCTCCAGCTTCTCAGCTGCTTTGACC TCCACATCTACTGCACTGGGCGCTTGGAGAAGGTAGGGAGATAGATTTCCTATACCTTGCTGCTTGCCTG CCAGGGCCAAAGGGTTCTATTGCCAGTATCTTTTTTTCCTAAGAACTTGGGTTCCAGAAAAATCAGCAAG CCTTTTCTTAGGCATCTTATCCTTGAAGTTTAATGCTTTTAACCCCGTTCCACTTCCCGCCAGAAGAGTG TCCTCCTCCCATCCCCACAGCACACCATCTTCTGCCTTTTCTTCTCAGCACCTGGAGCTGTTCCTGCAGC AACTCCAGGAGGTGGTGGTGAAGCATGCAGAGCCAGCGGTGCTTGAGGCTGGGGCGCATGCCCTCTACCT GCTCTGTAATCCCGAATTCACTTTCTTCAGCCGGGCGGACTTTGCCCGCAGCCAGCTAGTAGATTTGCTG ACTGACCGCTTCCAGCAGGAGCTTGAAGAGCTGTTACAGGTAGGAGCTGGGGCTGGACAATGGGACACCC CAAACAAGGGTGGGAGGGGCTGCAAGACGGGGGATGTGGACTGATGAGTTCTGGGGGAAATGCTATTGTG GATCTTCTTTCCTTCTCAGTCGTCCTTCCTAGATGAGGATGAGGTATATAATCTGGCAGCCACTCTGAAA CGCCTCTCTGCCTTCTACAAGTGAGTGGCTTTCCTCCTCTTCCCCATCCCGTTTTTACTGGTGTCTGTGG AGTTGGTTCCCTCTGTTCTTTTCCAGTATAACATTCCCCTTTCTCCCCCAAAGCACTCATGACCTGACTC GCTGGGAGCTCTATGAGCCATGTTGCCAACTCCTGCAGAAGGCTGTGGACACAGGAGAGGTTCCTCACCA GGTGAGTGGACAGGCTAGAGATGGGTTGGGGGCTGGGGGGCTAGATTTTAAGGACCTACGTTCTAGGTGG CCACTAGGTGTGTCAAGTCTCAGTGCGTGGAGTGGACAAAGCGAGGAAGATCAGGTGGGTGGGGGTCACC TCTTACATTCCTCTCTTAGAAGGTGGACTCTACTTCCAGGGTCTTCTTGGATTTCCTGGAAGCCAAGGAT AATATTGAGGGATTCTAGAAGACGTAGAGTAAATTAGGGAGCGGTGACATTTCTACTTCGTGTAGATGCC TCTGAAGAATGTCCAGAATATCACTCATTTTCCACTGGAAAAGGAATTAGCTCTCCCTTTACTCATTTTC TCTCCTCTTCACTGGTGTGTTTCTGGCTATCTGTCTCTCCCTCTCCTAACCCAAACCTCATTTTTCAAAC CCTTTGTTGTTACAGGTTATCCTGCCAGCCTTGACTCTTGTCTATTTTTCCATTCTCTGGACACTAACCC ACATTTCTAAATCAGATGCTTCCCAGGTGAGTGTGGGTCTTAGATGGGATAATGGGAACAGAGGAGTCTG TGTCTTCATTCTTCCCCTTCAAGCCACTGTGCCCACAGAAGCAGCTGTCGAGTTTGAGGGACAGAATGGT GGCCTTCTGTGAACTCTGCCAGAGTTGCCTCTCAGATGTGGATACTGAGATCCAGGAGCAGGTGAGTACT GACTCAAGTGGGAGCAACAAGGCGAGTATCCCTGCCCCCATTCCAAGGAGAAATTGACAGTGTCATTTCT ACCTTGGGAAACATTCTGTCCTTTTAAACATCCTTTCTTTTCCCCCTACAGGCTTTTGTCTTATTAAGTG ATCTACTTCTCATCTTTAGCCCTCAGATGATAGTTGGGGGCCGTGATTTCCTTAGGCCACTTGTCTTTTT TCCTGAAGCTACTCTCCAGTCTGAGCTAGCCAGCTTCCTCATGGACCACGTCTTCATCCAGCCGGGAGAC CTGGGCAGTGGTGCAGTGACTCTATACCTGGGGCTCAGTAAGGGCTGGGGCTTGGGTGTAGCTCAGAAAT ATAGGGCAGGAAGCTTAAGTCTGTGATTCCCTTTTGCCTTCCATGTGAGCCAGGTGATTCCCAGGAGGAT CATTTACAGATAGAGCGGCTACACCAGCGGCGCCGCCTCCTAGCCGGGTTCTGCAAGCTGTTGCTTTATG GGGTGCTGGAGATGGATGCAGCCTCAGATGTTTTCAAACACTACAACAAGGTACACCAAGGCCCTACAGA AATAAAAGAGAAGGGAGCAAGTTGAGCACAGCCATGACTTGGAAACATAATAGCACTACAGGTGGGGGAT ATCCAATGGTTTCAAGCTTAAAGGAGAAGTAGGAGGGAAAGGCACTGGGAGTTCAGAATAAGGGGACAAA GAAAAGAGACTTAATAGTAAATAATTATGGAGAAAAAGCTGTGAAGAAATTCACTGGAGGGGAAATAGCT TATTCTTCCTGTCCAGAAATATTTATTTTTCTCTGACCCCAGTCTATGGAATGGGGTAACATAAGAAGGA AAAAAGAATAAAAGAGGGATCAGTCCTCACAGGATACCTAGATAGGTGCCCCAGTTTCCTCAACCCACCA GTATTGATTTATGCTTATTGAGCACCTGCTGTGTGCCAAGCACCATTCCAGGAGCTAAGGCCACAGCAGT GAACAAAACACACAAGTTCCTATTTGGTGGAGCTTCCATTGTAGTTGGGGAGAGACAAATAGTGAAGGTG TCAGTGCTGTGTGAGAGGGCACTGTCAGGCTTACTTCTCTTCTCCAAGAATTGGGCTTGGGGCAGTGTTT ATTTCTTTTCTTTTCTTTTTTTTTTTTTTCCTGAGACAGAGTCTAGCTCTGTCGCCAGGCTAGAATGCAA TGGCACTATCTCGGCTCACTGCAACCTCTGCCTCCCGGGGTTCAAGCAGTTCTCCTGCCTCAGCCTCCCA AGTAGCTGGGATTACAGGCATGCACTGCCACGCCCAGCTAATTTTTATAGTTTTAGTAAAGACAGGGTTT CACCATCGCCAGGATGGTCTTGATCTCCTGACCTTGTGATCCGCCCAACTTGGCCTCCCAAACTGCTGGG ATTATAGGCGTGAGCCACCATGCCTGGCCTGTTTCATTATTTGTTTTTTATTTTATTTATTTATTTATTT ATTTATTTTTTGAGACGGAATCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACT ACAAGGTCCGGCTCCTGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCAC CCACCACCACGCCCGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCGTATTAGCCAGGATGG TCTCGATCTCCTGACCTCGTGATCCACCCGTCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCA CTGCACCCGGCCTTATTATTTGTTTACCGAAACTCTGATTTATTTTGGGCTTTGCACCTGTTGATTAGAG ATGAAGTCTAGTCTGAATTATTTTATGCTTCAGTTCATGTTTCCTACCTAAGGGAGTTTGGGAGGGAGAC ATGATTAGGGATGTGAAGGAAGAAACCAAATGGTCTTTTCCACCAGTCAGACATTACCTTCCCCACTCTT TCCCCTCAGTTCTACAATGACTATGGTGACATTATCAAGGAAACATTAACTAGAGCAAGGCAGATTGACC GAAGTCATTGTTCCCGAATCCTGCTGCTGAGCCTCAAGCAGGTGCGCCCTTCTGCCTTGAGGACATGCCA GCCCTGTTGTCCCCAAGTCTCTGTCTACCCTCAGCCACTCAGAATGTAAGATCCTTCAGAGCAGTAACTT TTCTTGTTTTGACTCTTAACTTTACGTGATAGGTTCCTTCATTCTTAGTAGACCCTTGGGAAGTATTTTT TTTTTAACTGACTGGGCAACCCCATCAAAAGTAACTTATCAGGACATTGGAACGCCAACAAGGAAGTGAT AGGGAGTTCCTAATTATTTTTCAGTCTGTTCTCTCTCAGCCTCCTTCAGTTACTATAGACGTTTTTATTT AATGTTATGGAGTAGAGGTGATTGGCTTTGTTTGCTGCAGGGGTATCGGGAGTTCCCTAGAAGGAAGGAA AGAGTAAAAGTAGTAGATGGTGTCCCAAGGCCTTTGGAATTTCTGAGTAGAGAAGAGAATGCTGGACTTC TCTGTTTCCGCCGTGTTCCTCCCTTTCCCACATGCACCATGTCTCCTGGACACAGCTGTACACAGAACTG CTGCAGGAGCATGGGCCCCAGGGCCTGAATGAGCTTCCTGCCTTCATCGAGATGAGGGACCTGGCCCGGA GGTTTGCCTTGAGTTTTGGACCCCAGCAGCTGCAGAACCGTGACCTCGTGGTCATGCTACACAAGTAGGA AGTATTGGTTGCAGGTTGTGTCCAGGGAGGGCTGGGAACGAGGTCTTGGAGGGAGGTCTCGGAGGGAGGG TATGTGTGTCAAGGCATAGCAGTCAGGTTAGGGGTGGGTATGCCTTTGGAGACAAGCTGGGGACGGGGGG AGGGTGCATTTGGACAGGATAGCTCAGGCCTGGGAGGGAAGTGGGAAGAGACTTTCTTCCTAAAATAATT CTGCCCAACCAAGGGAAGGCATCCAGTTCTCCTTGTCTGAGCTTCCTCCAGCTGGCTCCTCCAATCAGCC TCCAAATCTGGCATTCCTGGAGCTCCTTTCAGAGTTTTCCCCCCGACTCTTCCATCAGGACAAGCAGCTT TTGTAAGTTGGTGGGTGGATAGAGATGTGGATTAGGGAAGGGCCTGCTGAGGGCCCACTAGCCCCTTCAG GCTTTTGGTTCTACCTCTTTCATAGACTGTCCTATCTAGAAAAGTGCCTGCAGCATGTCTCCCAGGCACC TGGCCATCCCTGGGGCCCAGTCACCACCTACTGCCACTCCCTCAGCCCTGTGGAGAACACAGCAGAGACC AGCCCTCAGGTCCTCCCCAGCTCCAAGAGGAGGCGCGTTGAAGGTAGGGTGCTGTGTGTGGGTATGGGGG TGCCCAGCAGGTGGTCGTTGGCTGCCTGCAAACTTATTTTGTCAAGTATTTCATTGAGCATCTACTGTCA TTCAGGGTATTAATTTCTTTCAAGGTTTATCTAGTCAACTGAAAACCTGTATTAAAAATCAAATAGAGGG CCAGGTGTGGTGGCTCATGCCTGCAATCCCAGCACTTTGGGAGGCCGAGGCAGGCGGCTCACGAGGTCAG GTGTTCGAGACCAGCCTGGCCAAGATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCTGGGCA TGGTGGCGCGCATCTGTAATCCCAACTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAACCCGGGAGGC GGAGGTTGCCGTGAGCTGAGATTGCGCCACTGCACCCCAGCCTGGGTGACAGAGCGAGACTCTGTCTCAA AAAAAAAAAAAAGAAACTGATGATAAAACCAAATATTGAACTAAGAAATCTATACATTCAGAGTACCCTC CTCAACATCTTGTATTCCATTATTTTCGAAACCTTGGACACTTTTACACAACCTTCATTGTTTTTTACCT TCAGATCACCTGTTGTCTTATAAGTTGCCTAAGGTTTTGTTTTTGTTTTTGTTTATTTTTTGAGATGGAG TCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGGCGCCATCTCGGCTCACTGCAAGCTCTGCCTCCCAGGT TCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCACCCGCCACCACGCCTGGCTAA TTTTTTTTGAATTTTTGGTAGAGATGGGTTTTCAGCATGTTAGCCAGGATGGTCTTGATCTCCTGACCTC ATGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGTGCCTGGCTGCCTAA GGTTTTTTCTTTGCTTTTTAAAAAACAGCTTCATATTAAGCAACATTGTAGCAATCATTATTTCATATGC AAGTATGTGTTTTCTAGTGTACATTTTTAAATGGGACTTTATGTGACAGCTTTATTGAGATACAACTTAC ATATCATAAAAATCATTTTGTTTTTTTTGAGACGGAATCTTGCTCTGTCACCCAGGCTGGAGTCCAGTGG CGTGATCTCGACTCACTGCAACCTCCGCCTCCCACGTTCAAGTGGTTCTCCTCCCTCAGCCTCCTGAGTA GTTGGGATTACAGGCGTCCACCACCACCCCCAGCCAATTTTTGTATTTCTAGTAGAGACAGGGTTTCACC ATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTTGTGATCTGCCCACCTCAGCCTCCCAAAGTGCTCCCA AAGTGCTGGTGAGCCCCCGTGCCCAGCCAAAAATCACCCTTTTAAAGTGTACACTTAGCATATTCACTGA ATTGTACAACCATCACAACTATCTAATTTTAGAACATTTTCATCTTTCCAAAAGAAACCACATACCCATT CGCAGTCTCTGCCCATCTTCCCAGCCTTAGGTAGTATCACTAATATAATTCCTATCTCTTGATTTGCCTA CTCTGGACCTTTCATATAAATGGAATCATATAATATGTGGCCTTTTATGTCCAGCTTCTTTCACTTAGTA CAGTGTTTTCAACGTTCATCCATACTTTGACATATATCAGTATCTCATACCTTTTTATGGCCGAAACAAT ATGCCATCGTGTGGATATGCCACATTCTACTTATCCACTTGTCAGTTGATTGACATTTGGGCTCTTTTAT GCATAATGCTGCTAAACATGCATGTACACATTTTTGGGTGGATGCATCTTTTCAGTTATCTTGAGATATA CCTGGGAGTGGAATTGCTGGTTCGTATGGCAATTCTATGTTTAAGTTTTTGAGGAACTACCACACTGTTT TCCAAAGTACCTACACCAGTTTACAATCCTACCAGCAATGTATGGGGACTCTGGTTTCTCCATGTTGTCA CCGGTACTTATTAATGTCTTTTTTTATTTTATCCATCTCCTAGTAGCTGTGAGGTGTGGTATCTCATTGT GGTTTAAATTTGCATTTCCCTAATGACAAATGGTATTGAGCATCTTTTCATGTGCTTATTGACAATTTGT TTATCTTCTTTGGAGAAATGCCTGTTCTGATCCTTTAGCTATTTTAAAATAGGGTTGTCTTTTTATTGTT GAGTTGTAAGTATTCTTTATATATTCTAGACATGAGATTCCTTATGATATGCAAACATTTTCTCCCATTT TTTGGGTTGTCTTTTCGTGTTATTGGTGGTGTCCTTTGAAACACGAAAGTTCTTGAATTTGGTGAAATCT AACTTTTTCCTTTTGTTGCTTGTGCTTTTGGTGCCATATCTTGAGAAATTGTTGGCTAATTCATGAGGTC ACAAAGATTTACTCCTGTGTTTTCTTCTAAGAGTTATAGTTTTAGCCGGGCATGGTGGCTCACGCCTGTA ATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACGAGGTCAGGAGATTGAGACCATCCTGGCTAACA CGGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGCCAGGCATGATGGTGGGCGCCTGTAGTCCCA GCCACTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGCGGAGCTTGCAGTGAGCCGAGATTG TTCCACTGCACTCCAACCTGGGCGAAAGAGCGAGATACTGTCTCAGAAAAAAAAAAAAAGTTATAGTTTT AGCTCTTAATTTAGATTTGTGATCCAGTTTGAGTTAATTTTTGTATATAGTGTGAGATGAGGTGTGATTT CCTTTTGTTGTCTTCTGTGTACCACCTAAAATTATGTCTTATACCACCAGGAAACTCTGCTTTAATTAAT TAACTAATACATAGTTGTTAGGGACCTACTATATGAAAGATACTATGTTCAGTGTTTTAGGGGCCATGAA GAATCCAGCAGCTTATTATCTAATAGGAAAGGGAGAAATTTTAATTATGGAAAACTCGAATTATGAACAA TTGCCAATAACTGCCTGAGGGCTTGTACAAGTGAATGCTGTAGAATTCAGAGAATGATGAGAGCTTTACC AAGAAGATGGAATTGCTGGAGCTTAAAAAATAAGTAAGATTTTTATAAGCTGAACAGAGGAAGGAGGAGC ATCATGGGCTGGCAGAATAGCACAAGCAAAGCATAGAAGGCTGTGTGCTGGGCGAAGCATGGAGGTACAT AGATCGCTCTGCCTAAAGCGTAGGGTTTGTGAAGGCAAGATAAAACTGAAGAGTTTGTTTTTATTCATTC AATAGATACTGAGCACCGTGTGCTGGGAATACAGCAGTGAACAAAAAAGCTAAACATCTCTTTCTTTAGA GAGTGTCAGATAGTAGTAAATACTATGGCAAAAAAGCAGGGAGGATTGTGAGGAATGCTGGCGGGGTGGG GATTGCAGTCTTACAAAGACTGATCAAGGGAAGACTCAACACAAATGTTGGGCAAAAAGCCTAAAAAATA AGTTAAAAGATACACTTAGGCACATTACAATTTTAACAGGTTTATTTGAGCATTCAGCAATTTTATCAAT CAGGCAGTGGCAGACCGCCAGCAGTTCAGGGCGCCACCACCGAGGAAACCAGAGGGGACACTTACAAGGT GTCTCTGGGAGCAAGACAAAGAAACTATTTGATTGGTTAGAGTGGAAAGTTCATATTTAGAGGTTAACTG ACCATAAATCTCTTGTTAGAGGTTAGTCGGTAGTTTCTGACTGGTTAAGCTGAAGTTTCCTGCTCCTAGG TTACACACAACACTTTCACTCTGAGTTGAGTTTCAGTTTGCTGACTTAGGAACCCAAAGTGCAGGAGCCA TTTCAGCCTATTGGCCTCCCAGCGAATTTTTTATAACAGGTGAGGGAGGGTGCTTAGTGTGTATCAGAAT AGCATACCAAAGGAAGCAGCCAGTCCTGAGGCCACGAGGGGATGGGGGGAATGTTCCTGGAATGTTCAAG GAACAGCAGAGAAGACCATTATGGCCAGAATGCAGATTGTAGGACATGAGACCAAAGAGGGTACAGGATC CCAGAGCATCTAAGGCAGAGACTGGCTTTTACTCTGAGTGCAGTAGGAAAAGCATTGGAGGATTTTGAGG AACGACAGGATCAACCTCTGTTTTTAAAGGATCCTTCTGGCTGCTGTGTCGAAAACGACAGGAGATGGGG GAAGGCTAGAAGCTGGGAGACCAGCAGGGAAGTTGTGGCTGTTGTAGGAGTCCAGCCCAAGATGCAAGAT AAGAGAGCTTCTTAGGCCGGGGGCAATGGTGGAGGTGCCGAGAAACGGCCAGATTCTGGGTGCATTTTCA AGGTATCTCTAATAGGCTTTTCTGACAAACTGGATGTGGGATGTAAGACAAAAAGAGCAGTTGAGGATGG CTCCAAGGATTTTGGCCTGACAGAAGGACGGAGTTGACAGTATCTGAGATGGGGAAGGCAGAGAGTGAAG ATAGCTGTGGGGGAAGGTTGGGGCATTGGTGTTGGCCACAGGATTACAGGTGGAGATGTTGAACAAACCA TGGGGGCTGTTAAGATGGAGTTCAGGGGAGAGCTGAAGGCTTGAGATCCCTTTGGGATGTGGCAGAGTAT AGGTGATATTTAAGGACATGAACGTGAGTGGCTGGGGAAGCCAGTGAAGATGGAGAAGAGGAGAGACTCA AGGGCCCAGCCCTCGGGCATCACACCATTAATAGCTCAGGGAGATCAGGAGGACACATCTGGGGCCCCAA GGTTTCCTCTCTGGGAATCCAAGCCCAGGGGTATGTCTCTTCCCTGTTTTCTGTAGCCCTCTGCTTAAAC CCATAGGCTGAGCGCTCACTTTGGGATTATTGTTTTTAGTGGAGAAGGGGGGTAATGGGAGTGTGACTGT TTACAGTTAACAGATGTTAATGTCAATTTGACTGATGCTTTAAAATAGTATTGCCTGGGTTTTCTCAGGC AGTGAGAATCTTTGGATTCTTTCTCCTCCTCTACCTTCTCCAATCTCTGCAGGAGATAAGTCCCCCTTCA TATCATATCAGTTATACTGGCATTGCAATCACTTCATTTTTTTCTAGCTTTTAGAAAATCCTGTCTCCCA TTTTCCAGTTCCTCCCTGGCCCTCATTTGTCCACTCTAGCCTCTGGTACCAAAACTCTATTGCCATTTAC AGCTTTTTCTGACCACAGAAATCTTGGATAGGATCTCAGATGTCTTCATTGGCTAACTCAGACCTCTCAA TCTTAGAGACTCCAACCCTCCCTCATGTTTGTGTTCACCTGTTCACCTGTTACAGTTTATCTGTGAAATG TATATTTATATATCTTTCCATCTTGTTTTCTAGGCTGTGTAGCAAGTTCTTCTTGGACAAGGCCTAGCTA CAATTCTCTAAGCCTTACTTTTTGGTACATAGGGCTATGTATTTAGTACATAGGGCTATGCCCATTTGAG GGCAACAGAATGAAGCTGTATAAGTTTTCCATTCTCTTCCTGATTGACTTTCCCTTACTAAATCCTGGGC AGGTCTTGGAAAGAGAGCACACCTGTCGCAGGCCCTGGGCTGTGGTTAATGTATGCATCTGCTTGGCAGG GCCTGCCAAGCCTAACAGAGAGGACGTCTCCTCGTCCCAGGAAGAAAGTCTGCAGCTGAACAGCATCCCG CCCACGCCCACCCTCACCTCCACAGCTGTGAAGAGCAGGCAGCCCCTGTGGGGGTTGAAAGAGATGGAGG AAGAAGATGGCTCAGAGTTGGATTTTGCCCAGGGGTGAGGCCATGGAGGGAATCTGGGTGTCTGAGTTCC CAGTTTGGTGTCTGGCTGCCTCCATCTTGCTGTTTCTGTTTCATGGTTCCCTGCTGTAGCACTCCCACAT TGTTGGGTTCTTCTCAAACCCTTCTCCATTGTTTAGCTTTAAAATGCATCTCTCTGAGCTTTTCTCCCCA TTAACACCAGCTGCCTTACATCCTCTTCTTACCCTTGATTTTTATCCCATCATTGATCCTGCTTCATTCC CAGCAGTCAGCCCGTCGCAGGCACCGAGAGGTCAAGGTTCTTGGGTCCACAATATTTCCAGACTCCACAC AACCCTTCAGGTCCTGGCCTGGGCAACCAGCTGATGCGGTGAGCTTTTCTCATCATCTCCTGTCTTCACT TCAGATCTGTCGCCCACTGTGAGGGGATTCCTGTCTCACCCATTGCCTCTCTGTGGGTGCTTTTTGGACT TGTTTTAGCCACAGAGCACTTCCTGTCAGCACAATCGGGAAACTACTATGCTGTACTTACTGCTCCCCAC TTCCCCCACCCCGGGTGTCTGAGAAACTCTCAGGGGTCCTCAAAGAACAGTTTGAAAAGCCAGTCTCTTT GCCCCTACATCAGACTCAGCCTTATGGAAGAGGACGAGGAAGAAGAGTTAGAAATCCAGGATGAGTCAAA TGAAGAACGGCAGGATACAGACATGGTGAGTAGACCACCCTGCCCTTCTCTCTCAAGAACTAGGGGCTGA TGTGCCAAAGAGAAGCCAGGGTGTTTCCCCTCTCTCCGCTCGGTGATGCCTGGTCTGGAAAAGCTGAATG AATCTAGAGTAAAGGAGGACAGGTTATATGTTGGTTTGGACTTTGCTTGGACTCTACTTTTATTAAAGAA TAAGGAAAAGAAATTACAAATACCAAAGAAGGTTGGAATAGCACTCTCGCTCCCAATCCCTAAATAAATA TTGTTGCCTCCGAACCCCCTAGGAATTGGAAGGAGTCCTAATGCTGGAGGTGGGAGGTCATTCAGTCCAA GTGGCAGCCCCTTCAGTGTCTCTCCCCACAAAACATGACCCCCTATCCTCCATCTCCCTACTAGAGGGCT TCCCCTCCCTGTCCCTTCCCTCACTCGCTTTCTAACCTAGCCTGTTTCCCCAGGAGGAACTGCTGCTGGT GTAGTGGGAAAAGCCTGGCATTTGGAGTGAGGTGACATGAGTGCTAGTCCTCAACTCCATGTCAAGTTAG CGCTTTTACCCCTCCTCAGCCTCCATTTTCTCATCTTTATTTTTTAATTATTTTTATTTTTTGAGGTAGA GTCTCTCTCTGTCACCTAGGCTGGAGTGCAGTGGCATAGTCTCGGCTCACTGCAACCTCTGCCTCCTGGG CTCAAGCAATTCTCCTGCCTCAGACTCCCAAGTAGCTGGGATTACAGGCACGCGCCATCATGCCCGGCTA ATTTTTTTTGTATTTTTAGTAGAGACGGGGTTTTGCCATGTTGGCCAGGCTAATCTCGAACACCTGACCT CAAGTGGCTCCACCTGGCTCAGCCTCCCTAAGTGCTGGGATTACAGGTGTGAGCCACTGCGTGGGGCCCA TTTTCTCATCTTTAAAACAGAGCTGTTAGCCTGTAGTTCCAGCAACTCGGGAGGTGGAGACAGGAGGATC AATAGTCCAGGAGTTGGAGTCCAGCCTGGGTAACATAGTAAACACGTGTCTCTTAAAAAAAAATCAACAA TTCAAAAATAAATAAAATGGACCTTTTACATAAAATATAAGGTAAAACCACCTTATAAATGGAAAAGGGC TATGTCTGAGTTCAGGCTACTGTAACAGAATTCCATAGACTGGTGTCTGACAAACAGCAGGTATGTATTT CTCACAGTTCTGGAGGCTGAAGTCCAAGGGTTGGGTTCTGGTGAGGGCCTTCTTCCAGCCTGTAGACTGC TGGCTTCTAGTTGTATCCTCACCTGGAGGAGAAACAGCAAGCTAGCCCTCTGGCCTGGGCTTAGAAGGGC ATTCATCCATCCATGAGGGCTGCACCCCTATGACCCAATCACCTCCCAAAGGCCCCACTTCCAAACAGCA TCACACTGGGGGAAGGATTTCAATATGTGGGTTTAGGGGGACACAGATATTCAGTCCATTACAGGCTGCG TATCTTCTGCTGTTATTCTTCTGTTCTCTTCTCTGCAGGGAAGGAAAAAGAACAGAATAGAACAGAACAG GGGAGTCTTGTGCTCAGAAGGAGAAACCAAACAATGATACCTTCAAACCAGAGACTAGAGAGCAAGGCCT CTGTTAAAATATTATTTCCTAACATCTCAGAAAAAATCCGTTTTCTGGTTTCCAAAACAAAGTCATTTAA GGGGCTCAGTCAGCAGGCCAAGAAGCGCTCTGCAGTCAGGCCTGATCCTGGTGCCCACACTGACTTCCCT CCCAGGAGGTGCCATAGGGGCCTGCTGTGCCCATATTTGTTCTTATGAGGCTGGGAAAGGAACTGGTTTT TTTATTTGCGAAGTGACAGGAGTGTGTAAAGGCCTTTTTGACTTTTAACCTCATTCTCTCTAGCAAGCAA GTAGCTACTCTTCCACCAGTGAGCGCGGGCTGGACCTCTTAGATTCTACAGAGCTGGATATTGAGGTGAG TGTCCCCAGAGCAGGAGTTATGTATCCTTCGGAAATGCTGGCAGGCAACCCGTGCACTCATCAAATTGAC AGGCCATAGCCTGGGGGTGGCCCTCTGGGCTGTCTCTGGGGCTTTGAGGGTAACCCAGGGGAGGATGGTC TGCCCATTGGCCCGTTGCTGTGTCCTGTGTATTCCTTTCATCTTTCTCATTATAGGATTTCTGACAGGAC TCTGGGCCCCTCCCCAGCTCCACTCCCTACCTCAAGAATGTGACCATTTGGAAAAGGCAAAGAGAAAAGG AGCAAAATGAAGCATTCCCCCAGGCTTCAGCCCTGGGCTCTGAGGGGAAAGAGTTGGGCATTGTTTTTCT AACCTAACCTTTCCCTCTGGGGTAGAGAAGCCGAGAGACCCTGTCCTCCCTTATGCACTGTGGCCCAGTC CCCTTGCCTTTTTCCTGTTCTGTTTGGAGTGGAGAAGGGCAGCACCTCTGTGTTTAATGGAAATAGCCCA TAGTCTCCTGGATTTTTGGAACATCTTTCTCAGCCTATTTTGTGTCCTAATGATTCGCTCAATAAACATG TTTGAATCCACACGT >gi|57863310|ref|NP_036579.2| cohesin subunit SA-3 [Homo sapiens] MSSPLQRAVGDTKRALSASSSSSASLPFDDRDSNHTSEGNGDSLLADEDTDFEDSLNRNVKKRAAKRPPK TTPVAKHPKKGSRVVHRHSRKQSEPPANDLFNAVKAAKSDMQSLVDEWLDSYKQDQDAGFLELVNFFIQS CGCKGIVTPEMFKKMSNSEIIQHLTEQFNEDSGDYPLIAPGPSWKKFQGSFCEFVRTLVCQCQYSLLYDG FPMDDLISLLTGLSDSQVRAFRHTSTLAAMKLMTSLVKVALQLSVHQDNNQRQYEAERNKGPGQRAPERL ESLLEKRKELQEHQEEIEGMMNALFRGVFVHRYRDVLPEIRAICIEEIGCWMQSYSTSFLTDSYLKYIGW TLHDKHREVRLKCVKALKGLYGNRDLTTRLELFTSRFKDRMVSMVMDREYDVAVEAVRLLILILKNMEGV LTDADCESVYPVVYASHRGLASAAGEFLYWKLFYPECEIRMMGGREQRQSPGAQRTFFQLLLSFFVESEL HDHAAYLVDSLWDCAGARLKDWEGLTSLLLEKDQNLGDVQESTLIEILVSSARQASEGHPPVGRVTGRKG LTSKERKTQADDRVKLTEHLIPLLPQLLAKFSADAEKVTPLLQLLSCFDLHIYCTGRLEKHLELFLQQLQ EVVVKHAEPAVLEAGAHALYLLCNPEFTFFSRADFARSQLVDLLTDRFQQELEELLQSSFLDEDEVYNLA ATLKRLSAFYNTHDLTRWELYEPCCQLLQKAVDTGEVPHQVILPALTLVYFSILWTLTHISKSDASQKQL SSLRDRMVAFCELCQSCLSDVDTEIQEQAFVLLSDLLLIFSPQMIVGGRDFLRPLVFFPEATLQSELASF LMDHVFIQPGDLGSGDSQEDHLQIERLHQRRRLLAGFCKLLLYGVLEMDAASDVFKHYNKFYNDYGDIIK ETLTRARQIDRSHCSRILLLSLKQLYTELLQEHGPQGLNELPAFIEMRDLARRFALSFGPQQLQNRDLVV MLHKEGIQFSLSELPPAGSSNQPPNLAFLELLSEFSPRLFHQDKQLLLSYLEKCLQHVSQAPGHPWGPVT TYCHSLSPVENTAETSPQVLPSSKRRRVEGPAKPNREDVSSSQEESLQLNSIPPTPTLTSTAVKSRQPLW GLKEMEEEDGSELDFAQGQPVAGTERSRFLGPQYFQTPHNPSGPGLGNQLMRLSLMEEDEEEELEIQDES NEERQDTDMQASSYSSTSERGLDLLDSTELDIEDF Vaximmutor TBX21 Homo sapiens 30009 7019549 AC015674 NP_037483 9606 17 41179682 41192555 + T-box 21 5.92 55747.12 535 Also known as TBET; T-PET; T-bet; TBLYM >gi|157713538:41179682-41192555 Homo sapiens chromosome 17, alternate assembly HuRef, whole genome shotgun sequence GCGGCCCGCTGGAGAGGAAGCCCGAGAGCTGCCGCGCGCCTGCCGGACGAGGGCGTAGAAGCCAGGCGTC AGAGCCCGGGCTCCGGTGGGGTCCCCCACCCGGCCCTCGGGTCCCCCGCCCCCTGCTCCCTGCCCATCCC AGCCCACGCGACCCTCTCGCGCGCGGAGGGGCGGGTCCTCGACGGCTACGGGAAGGTGCCAGCCCGCCCC GGATGGGCATCGTGGAGCCGGGTTGCGGAGACATGCTGACGGGCACCGAGCCGATGCCGGGGAGCGACGA GGGCCGGGCGCCTGGCGCCGACCCGCAGCACCGCTACTTCTACCCGGAGCCGGGCGCGCAGGACGCGGAC GAGCGTCGCGGGGGCGGCAGCCTGGGGTCTCCCTACCCGGGGGGCGCCTTGGTGCCCGCCCCGCCGAGCC GCTTCCTTGGAGCCTACGCCTACCCGCCGCGACCCCAGGCGGCCGGCTTCCCCGGCGCGGGCGAGTCCTT CCCGCCGCCCGCGGACGCCGAGGGCTACCAGCCGGGCGAGGGCTACGCCGCCCCGGACCCGCGCGCCGGG CTCTACCCGGGGCCGCGTGAGGACTACGCGCTACCCGCGGGACTGGAGGTGTCGGGGAAACTGAGGGTCG CGCTCAACAACCACCTGTTGTGGTCCAAGTTTAATCAGCACCAGACAGAGATGATCATCACCAAGCAGGG ACGGTGAGTGCGGCGCGCCGGCCCTTGGGGCCTCTGTGCCCGCGCCGGAACAAGAACGTCTCGTCTGTTT TTCTGGCTCGACAATGCTTCTGACTCCGTGTCCCTCACTGCTTTGGCTTCAGCGTAGGGAGACAGGGGAA TGGGGTTGTTAGGAGGACAGGGAAAGCTCCGGAGGGGCGTCTGTGCCCAGGCTGTTGCACCAACAGCCAG AGGACTCACAAGGGAGACGGGTGAGTGCGGGACAGTGAGAAGTCACCTTGATTTAGGGGAAGGGTGACTG TGGCTTCACCTAGAATTGGTGTGCGCCCCTGCCCCACTCTCTACTGTAGAGGAGTCGCAGCGGGCAGTGA AAGCCTGTGCTCTGGGCGGACAGGACGCCTGGGCCTCCTGTGTGGGGAAACTGGAGGGGAAGGGAGCCCC TTATCTCCGGGCCCCCTGCGCCCACCTCCCCCGGCTCCTTTGCTGCTGGTGTGCTCAGGTCAGCTTTAAT GGTGGTAGTGGTGGTGGTAGCGGTGGTGGTGGTGTGTGTGTACGGGGGGAGATTGGGATTTGGTGACATG GAGAAGCAGTCGCCAAGTTTCCTTTCCGGTCTTACTTTGAGATCATATGTCTGGTGTGTGTGTGTGTGTG TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTGTGTGTGGGTGTGTGTGTATGGGGGCTAGTGCA GTAAAGCTTGTAGGGGGCACAGATCCCTTCCAGGCACAAATGCCCACGGGCTGGGCAGATGAAGCTCACC CAGGGGTCCAGCCTGGTAGCCAGCCCCACACTGCACCCTTTGAGGCTGGTCCAGTGAAAACCTTCCCCAC ACTCCTGTCCAGAAATTCACCGGTTCAGCCTGGAGAAGTGGGGAAGGGGTGTCCCATGGCTTCATGGCTC AGGGTTCCTGAGCCCCGTGCGTGATGGGGAGAGTTTGGGGCTGAGGGTGCTGCTTCCGGATAGAGCCTCC TGCGCAAGGAAAGAAACAGAAACCTGTGACTTGTGTGGTATTTGTTTAGTAAGCAACCCCCGGAGTGGAC TGTGTCTGATGTGGGCTGTGCGCACCCACCCTTCCCAGTGCGGCCCATGTGAGCAGGGGACCAGCGAGGA CCAGTGTGGAAGGGCTGTTGTCATTGGTGGACCTGGGATGCTGGGTCCCAGGTCCGAGAGGTGTGGATAC CAAACGTGGGGCTTGGGGTGGAGGGGAGAGGGAGAAGGCCATGTTGGACCCCAGAGGTTGGTATTCGATC TGGGCATTGCTGGAACATTCTTCCCTCCAGATGATTTTTGTGGGGTAGCCTGGGACTGGGGAACCTGTTG CCAGCCACAACTGGCTTCAAGTTACTGAGCTGTTCCACTTTCCCTGGGATGAACCCAGGAAAGTTGGCGT GTTCTTTGATGGCTCAGCCTTCATCTCTTAAGCCTTCTGATAATTTCTCTCTGCTCCCCCCAACCCTAGT CACCCTTTGGGTGTACCCAGACACAGCCCAAGCTTCTGGTCCCAATGTGTCTGAATTGGAAGGGACCCAG AAGATCCTGTCCCTCTCACTGCACAGACAGGGAAACAGGCCCAGAGATAGCAAGAGACTCTTCCAAGGTC ACACAACACACTCATTGCAGGCAGAGCTGGCACTACAGCCCAGATAGTATTTGCTTGGGTTTCTTTCTCT GGCCCCCTACGGCCACCAGTAAAAACAGCCACCCACGCTCTCTGAAGGCCCTGATGCATTCAGAGTGCTT TCACTTCCATTATTTCACTGTGCGTTAGGTAGGCCAAGCAGTCTCCCCATTTTACAGTTGAGGTCACTGA GTTGCAGGGTCAGCTAGGGGCTTGCACTCCATCACACAGCAGACCAGAAGTGGGGCCTCTTAACCTTCCA CTCTGTTGTGTGGTCAGGAGGACTTCCCACCCTGGGCCCTTGCTGGGGTCTCCCCACAGCCTCTGCCAGG CCTCTGCCTCCTCCTGCCAGTCTTGAGGGATGGGGTGGATGGAGCATCAGCCAGCACTCTAGGGAGTTGG CCAGTGCTAGGGGGCTGCCTCTCTGCCTGTAGAGCCAGCTTCAGGGAGGCTGTAGAGCACTTCAGGGAGG CACTTGAGGGGATGATTCTCGAAGTGTGTATCACCATCTCTACTCCCATGGCCTCCTTTCCTTGGTGTTC CTGCTGAGTAATTCTCACTTGACAAGTTTTTTTGTCCCCCTTTTAGATACATACACTTACATTTTTTTAT TATGAAAAATTCCACTACATACAAACATGGACAGAAGAATGTAACAGACTTCCATCCCCAGCTTCCACAG TTGTCAATATGGGTCCAATCTTGTTTCTGCATCCTCACTTCGCTTCTTCTGTCTGCAACTATATTAGTAA TATAGTAATTACTAAGTACATTCTAGATATTATTTTATCTCTGCACCTCTAAAAATGACTTTTTAAAATG AATGCATTACCATTAGCAAACCTGAAAAGAATTTCCTAAATATCACCAAGCTTCTCTGATTGCTTCATAA ATGTGTGTGTGTGTGTGTTTTCCAATCCTCACACTTTTTGCTCCATTCTGAGGGGCTCCATACGCGGGGT CCCTGTTGAAGGAGGCAGTGGCTCTTCACTGTTTCTTTTGGTGCTTTTTCTTGGATCAGGCTTCATAGAC CCCACGCTGCCATGGAGGGGACAGAGGACACACAACCCCAATTCAGTCCCACTAGCCCCAGGTTACCTCC ATACCTTCTCAGCTTGGGCTCCTGAGATCTGGGAAATCAGTGGCCATTTCCTCTCAAAACAGGCTTTTGG GCTTATAGCACAGCTTCCTTTCCGTCTTCCTGCTTTTGTGGGCTGGCCTATCCCTCCGTGGGTTCCAGTG ACCCACCAGTCACACCACATCACCCATGACCTTCTTCCTTTTTGAAAGGTTCTCCCACCTCAGGCCCACC TACAGCCTACAGCCCCACGGCTTTTTGCCTTGAGCTGACTCACGCCGTCCCGGATTCTTCCTCTCCCACC CACCCTGGATCTATCCCTCGGTATTGACCAGCGCCAGTGGCATGCTCTTCCCAACGGGCAGAAATGGGGC AGGGCACAGGGGGTGATTCCTGGGTCTGGAGATTGATCAAGGCAGCTCAGATGGGGCTGAGAGAGGGGAA CTGAGAACAAAACGGTGGATTATCCCAGTAGGAGACTTTGGTGTCTACGTTTGTGTCTGGAGATTTGGGG CAGCCTGATAATGCCCCACACAGGCTTGTGCGAGGGTTTCTGTGTGGCAGTGTGTGTGTGTTGCGAATGT GTTGGCGAGTATATAATAGTTGATGGTAGACATTCTCAGTAGAGGAAGAGAGAGGCCTGTGTCTCTGTGG GTGTGTGGGTGTGTATTAATTGCTGGCAATCCCCACTTTCTGGGTGTGATTGGTTGATTATTATAATTAA GATAACTTTGGGTAGCTGTGTTGTGTTAAGCTCAATCTGCATTTGGATTTGTGCATGTGAAGACAGAGGT TGGTAAAACAACAATTTCAGTCCCACACTATCCTTGCTATCCTCACAGTCATTACAATTTTTTTTTGTCT CCACTTTCCCAACTCCAAGAACTTTAATTTTTTTATTTGTAATATTTTTCATGGGCCAGGAAGTGGACTT GAACCTCCCACATAGATAACTTTAGTCGATAACTTTCTACATCTTTTTTTTTTTTTGAGATGGAGTCTCA CTCTGTCCCCCAGGCTGGAGTGCAGTGGCTCAATCTTGGCTCACTGCAACCTGCACCTCCCAGGTTCAAG TGATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCAGAAGCCACCAAGCCCAGCTAATTTAT GTATTTTTAGTAGAGACGGGGTTTCGCCATGTTGGCCAGGCTGGTCCCAAACACCTGACTTCAAGTGATC CCCCGCCTCGGCCTCTCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCTGGCCCAACTTTTCAGAT CTTAAAAAATTTTTTTTAATTTATTTTTTATGGAGACAGAATATTACTGTGTTGCCCAGGCGGTCTCAAA TCCCTGATCTCAAGCAATCTCCCGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCATTGTGC CCAGCTAAAAACATTTTTTTTAGGCTTAAGCATGCTTAACTGGTTTCATTTGTCTTGGGGCAAGTGAAAA CAACTTTAATTAAAAATTTTTTTTAAATGAGACATGGTCTTGCTCTGTCACCCACGCTGGAGTGCAGTGG TGCGATTATGGCTCACTGCAGCCTCAACCTGCTGGGCTCAAGCAATCCTCCCATCCCAGCCTCCTGAGAA ACTGGGACTATAGGCGCACATCACCATGTCCGGCTAATTGTTTCTATTTTTTGTAGAGATGGGGTCTCAC TATGTTGCCCAGGCTGCAGCTTTGCTTTTTGTCTGGCTGTTTGCCAGTGGCTGTCTGAGTCTATTAAGTG GATCTGAGGTTGGTAAGACGAGGAGTTCCCTTGATGCCCAAGCACATTTTGTACTTTGGTAGGTACGACA CATTGCCAGAATACCCTCCCAAAAGGTGCTACCAATTTACGCCCACACCAATAGTCTATGAGAGGACCCA TTTTCTCACAGCCTCATCAGCAGTAGATATTATCACATTTTTTTTTTTTTTGAGACAAAGTCTCGTTCTG TTGCCCAGGCTGAAGTGCAGTGGCATGATCTCTGCTCACCGCAACCTCCGCCTCCCAGGTTCAAGCGATT CTCCCACCTCAGCCTCCCAAGTAGCTGGGATTACCGGCATGCACCACCACGCCTGGCTAATTTTTGTATT TTTAATAGAGATGGGGTTTCACCATGTTACCCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCCACCT GCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCACGCCGGGCCAATATTATCAATTTTTA AACATTGACCAATATGACTGAGAAGAACTATCTATCTCCTTGCTATTTTAATTTGCACTTAATTACAGTG AAGCAGAGCCTGTGCCAGGAGCTCCGAATCTGGGAGAGGTATGACGACCCTGTGAACCATCCCAAGGCTA CTTAGTTCTTAGGGCCAGCAGAGGAATTTGGGGTCTTGGACCCTGTCTTATGAGGGTGGGATGGTAAGTG GCCTTTAGGGACGCTCAATTTGACACCAGACCCATCACCACTCTCGGTTCCTTCCCAGCAAAGTGTCATG TAAATCAGGGGCTATTTTGGGATTCCAGCTGGTGGTGATTCTAGACTTTTAGGGAGAAACCGAGATCTCC TTTCTCTTCTCCCTTTTCCCCAGCCTCTTCCTGTGTCTCCATTTCCCTCTACTAGTGATAAAAACAGGAT GAGCTGGGCATGGTGGTGAGCACCTATAACCCCGCTCGGGTGACTCGGGAGGCCAAGGCAGGAGGATTGC CTGTGCCCAGGCATTTGAGCAGAGCCTGTGCAGCATAGCAGACTCCATCTCTTAAAAAAAAATCAGTATG GCCTGGGCGCGGTGGCTCACCCCTGTAATCCCAGCACTTTGGGAGGTCGAGGCCAGTGGATCACCTGAGG TCAGGAGTTCGAGACCAGCTTGATTAACATGGTGAAACCCCGTCTCTACTAAAAGTACAAAAATTAGCCA GGTGTGGTGGCGGGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATAATGGCGTGAACCT TGGAGGCGGAGCTTGCAGTGAGCCAAGATCACACCACTGCACTACAGCCTGGGCAACAGAGCGAGACTCC GTCTCAAAAAAAAAAAAAAAATTAGCCAGGCGCAGTGTTGGGTGCCTGTAGTCAAGGAGAATCTCTTGAA CCTGGGAGGCAGAGGTTGCAGTGAGCCGAGATCACACCACTGCACTCCAGCCTGGGGGACAGAGCGAGAC TCTGTCTAAAAAAAAAGAAAGAAAAAAATGAATATGAAGAAATGGGGACCACAGAGCACAGCATGCACTC TGCAGTCAGAAGACCTCACCTCTGCTCTCCCACCGAGGACCGTCACCAAACCTCTCGGAGCGTCAGTTTC TTCAAATATAAAATTGGAAATAACATTTTATTTTTTATTTTTTCTTTGAGATGGAGTCTCACACTGTTGC CCAGGCTGGAGTGCAGTGGCGCCATCTCGGGTCACCGCAACCTTCGCCTCCCAGGTTCAAGCGATTCTCC TTGCCTCAGCCTCCAAAGTAGCTGGGACTACAGGTGCATGCCACCACACCAGGCTAATTTTCTGTATTTT TAGTAGATATGGGGTTTCACTATGTTGGCCAGGCTGGTCTCGAACTCCTGAGCTCAGGAAATCCACCCTC CTCGGCCTCACAAAGTGCTGGGATTACGAGTGTGAGCCACTGTGCTGGGCCATCTTTCGAACTTTTTAAC AGCTTTCAGAGGAAAGATGGACAGGAGTTAGACTCTCCATTTACATGTGAAGAAACTGAGATTTGGGCTG GGTGCAGTGGCCCATGTCTATAATCCCAGCACTTTGGAAACCTGAGGCGGGTAGACCACTTGAGGTCAGG AGTTCGAGACCAGCTTGGCCAACGTGAAACTCCATCTCTACTAAAAATACAAAAATTAGCCGGGTATGGT GGCACATGCCTGTAGTCCCAGCTCTTGGGAGGCTGAGGCAGGAGAATCACTTGAACCTGGAAGGTGGAGG TTAGAGTGAGCTGAGATCACGCCACTGCACTCCAGCCTGCATGACAGAGCGAAACTCCATTTCAAAAAGA AACTGAGATTTGGCTTGAGAATTAACTTATCCAGGGTCATAGGGTAGTAATCTATTTTTCTCAACTCAGG AAAGTCAGGGGAAGACAGGCGTGTGACAGCACCCTTCTAGGACACCTTCCCAAGGTTGTTAGGTAAGTTG TCCTGGGGACCTCTGGGGATTAGAAGTCCCTCAAAAACAGTTCCTAGGCCCCTGGAACCCTCCCTCCTCC AGGTTTCCTGACTCAGATAGGTCCCTTTTTCCTCATCCTTCAGGGCTTCTCATGAGAGGAGGGGGAAGTG TGTGGAAATGAAAAATAATTCTGATGAGGATTAGCTTCTGCCATTCTGAAAACTCTGCTCTTCCTGCTTG TGTTAGAAGAGAAAGGCTAAGCCATTGGAGCTGGTGATGGAATTGGTGCTGGTGGAGGTGGTGGTTGTAG TGACGGTACTACTGGTGGTGGTTGTGATGGTGAAGGTGGTGGCTGTAGATGGTGGTGCCCGTGCTGGTGC TGGTGGAGATCGTGGTTATCGTGATGGTGGAGGGGGCAGTTGTAGTGACGGTGGTTGTGATGGTGGTGAT GGGTGGTTACAGTGCTACTGGTGGTGATGGTGCTGGTTGTAGTGGTGGTGGTGACGGGGCTAGTGAAGGT GTTAGTGGTGGAGATGGTGATGGTAGAAGAGTGCTTCTTTTCTGAATCTGTTACTCTGGCACAACAGCGG AATCATACAGCATCCCACCCCCTAACTTCCTCTACTTTTCCTGGAAACAAGGACTTTGAACATGAGCCCC GTGTTCAATAGAGCCTTCTTTCAGGTGGATGGGAAGCTCCCTGTTCAACTAGGATGTGTGCAACTTCTCT AAAGGCTGAGGGCAGACTATACCGCAGGTGGCAGACCCAGGAGCATGGGAGAGGGAGGGCCTGTGAGTGG GTGTCATGGGTCTGGGAGTGTGTTAGCACTGTGAGAGCCACGGCAGAGAGGGTGGCTGAGGAACTGAGGC GTGAGGACCTTCCAGGAACTGCCTGTGGAGAGCTGGTGGTGCCCCCTCCTAGGAAGCAGCTAAGGTTAGA GACAGGGGTGTGCAGGTAGATGTCGAGCAACTGACCCTCTGAAAGAACATTTTAAAAAACTCTGATTTGT AGTGTTTGCTGATCCCTGTGGTGTAAATACTCCTGCTATGGATGATTTCAAGCGACTCATGTGAGTCATT GAACACAGAGCCGGGAAGAGACTCACAGTGCACTGTGATTTAGTACCTCCTCCATGCAGCTGCGATAGAC GGAAGTAACCTCGACAGTGCAGACAATAGTAAAACGTAAAATAACTGGGCAGTGATGATTTTTTAGTTTT TACTCCTTTTTTTTTTTTTTGAGATGGAGACTTGCTCTTGTCATCCAGGCTGGAGGGCAGTGGCGCGATC TCAGCTCACTGTAACCTCTGCCTCCTGGGTTCAAGCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTAGGA TTACAGGCGCCCACGACCACACCCAGCTATTTTTGTTGTTGTTGTTGTACTTTTTAGTAGAGACGGGGTT TCGCCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCGCCCGCCTTGGCCTCTCAAAGT GCTGGGATTACAGGCATGAGCCACTGTGCCGGGCCTACTTTGACTGTTAATATGTCTTGAATTGCAAGTT TATATAATTTAATTTTTAATTATGGCTGTGTTTAACCACTGGCTGGCAAACTCCCTAAACACCTTCCAGC TGGTTCTTGTGAGTGGGAGGAAGCCGGCTACAGCACACCACTGATGCCTGGGCACTGTTGCAGGGGGGAC TGGCTGTCAAGCTGGAGCTGATGGGTGCTGGGGGCTTTCTCTCTTCTCTCCAGGCGGATGTTCCCATTCC TGTCATTTACTGTGGCCGGGCTGGAGCCCACCAGCCACTACAGGATGTTTGTGGACGTGGTCTTGGTGGA CCAGCACCACTGGCGGTACCAGAGCGGCAAGTGGGTGCAGTGTGGAAAGGCCGAGGGCAGCATGCCAGGT GCGCGCGCCCCTGGGAGCGGTGGGCTCTGTTTCGCTGGGACTGGGCGCCCCCTGGTGGGCCCACCAAGCC CCTACCCCTAATTCCTAGACCTTTAACCCCCTCCCACTCCATCCCACGCCATTGCATCCCTCCTGTTTCT GGCTTCCTGCTTTGCTCTAGCCTGTCCTCTGCTGAGTCCTCTGCCCTTCCCTGCCTGGTCCTCCCCCTGT GTCCTTCCTTACGTCCCTCTCGGGACAGGCAAAGCCCTACATCACCAGGGTTCTGTCCCGGGGGCTGCAT GTCAAAGAGGTGAACTGTCCACAGGAAACCGCCTGTACGTCCACCCGGACTCCCCCAACACAGGAGCGCA CTGGATGCGCCAGGAAGTTTCATTTGGGAAACTAAAGCTCACAAACAACAAGGGGGCGTCCAACAATGTG ACCCAGGTAGGACCTGCTCTTCAAAAGGTAGCCTCGCCCTGCTCCCCACCCTGGGTCTGAGACCTCCAAG GCCACAAGGGTCCTGCGGGGCCAGTTTGGTTCATTTTTTCTTCCTTCCTACAGGAAGGAAGGTTCGTTTT TCTTCTGTCCTAAACGAAGGGTCATTTTTCCTCCTTCCTAAACTCTGGCTGTTTCTCACCCACCTTGGGA GGAAGATGAGATGGGATGAAGCTGAATCTTGGACGGGGGTCATATTCAGGCCACACAGGCCAACCCAGCC TCAGGGTTTCTTCACCAAATCATGGGTTGCCATTGCTCCGGCACCATGAAAGGCACAGAGTTGGCCCTGT GGTCCCAGGGAATAGATGCCGAGTTTCTCTAGGTTGAACAATCCTGTTAAAAGTGCCCTTGCTGGCTGGG TGTGGTGGCTCACACCCATAATCCCAGCACTTTGGGAGGCCAAGGCGGGTGGATCACAAGGTCAGGAGTT CAAGACCAGCCTGGCCAAGATGATGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAAGCATGGTG GCGGGTGCCTGTAATCCCAGCTACTCAAGAGGCGGAGGCAGGAGAATCGCTTGAACCCGGGAGGCAGAGG TTACAGTGAGCTGAGATCATGCCATTGCACTCCAGCCTGGGTGACAGAGTGAGACTCCGTCTCAAAAAAA AAAAAAAAAGAAAAAAGAAAAAAGAAAAAAAAGTGCCCTTGCCCTAAAGCCCTGTTTGTGCTGATACCTT GGTCTCAATTGACATCTGGAGGTAGAAGAGGGGGCAAGAGGCTAGAATTGGTGCCCCAAAGAGTCCGGAC ACTGGAGTTGGAAAGCTTGGGGGAAGTTACATGGTGGCAGAGCAGGGGAATGGACAGGATGACCTCAAGG TGCTTGCTAGCCTCACGTGGGGCCAGCTGTTGGTGGGGGTGATGGGATCCTCGAAATCCTTCCTCCCCAC CCCTACAACCGTCTTGCTCTGTCTACAGATGATTGTGCTCCCAGTCCCTCCATAAGTACCAGCCCCGGCT GCATATCGTTGAGGTGAACGACGGAGAAGCCAGAGGCAGCCTGCAACGCTTCCAACACGCATATCTTTAC TTTCCAAGAAACCCAGTTCATTGCCGTGACTGCCTACCAGAATGCCGAGGTGAGGGCTGCCTGAGCCCCG GTGGGGAGGAGGGCAGAGTGGGGCCCACTGTCTTCCTTGGGAGGGATTTGGAAAGTTCCCGAGCCCCAGA CTCAGGACTCAGGTGACTCTATTTCCCTTCTCTCTAGATTACTCAGCTGAAAATTGATAATAACCCCTTT GCCAAAGGATTCCGGGAGAACTTTGAGTCGTAAGTGCCACTGGGTTCAACTCAGCTTTGGTCCCTCCTGA GACACATCCTCTCCCTGCCCCTGAAAACAGGAGGGTGGGGGACAGATGCTACAGGTGGGCAGGCCAGGGA AGGAGGGTCGGAGAAGGAATGTGTGAAACAGGTAGGCTCACAGGTGACTGGTTCTGCTTGTGACCCGTTT TCTTGCCTTCTATTTTTTTCTAGCATGTACACATCTGTTGACACCAGCATCCCCTCCCCGCCTGGACCCA ACTGTCAATTCCTTGGGGGAGATCACTACTCTCCTCTCCTACCCAACCAGTATCCTGTTCCCAGCCGCTT CTACCCCGACCTTCCTGGCCAGGCGAAGGATGTGGTTCCCCAGGCTTACTGGCTGGGGGCCCCCCGGGAC CACAGCTATGAGGCTGAGTTTCGAGCAGTCAGCATGAAGCCTGCATTCTTGCCCTCTGCCCCTGGGCCCA CCATGTCCTACTACCGAGGCCAGGAGGTCCTGGCACCTGGAGCTGGCTGGCCTGTGGCACCCCAGTACCC TCCCAAGATGGGCCCGGCCAGCTGGTTCCGCCCTATGCGGACTCTGCCCATGGAACCCGGCCCTGGAGGC TCAGAGGGACGGGGACCAGAGGACCAGGGTCCCCCCTTGGTGTGGACTGAGATTGCCCCCATCCGGCCGG AATCCAGTGATTCAGGACTGGGCGAAGGAGACTCTAAGAGGAGGCGCGTGTCCCCCTATCCTTCCAGTGG TGACAGCTCCTCCCCTGCTGGGGCCCCTTCTCCTTTTGATAAGGAAGCTGAAGGACAGTTTTATAACTAT TTTCCCAACTGAGCAGATGACATGATGAAAGGAACAGAAACAGTGTTATTAGGTTGGAGGACACCGACTA ATTTGGGAAACGGATGAAGGACTGAGAAGGCCCCCGCTCCCTCTGGCCCTTCTCTGTTTAGTAGTTGGTT GGGGAAGTGGGGCTCAAGAAGGATTTTGGGGTTCACCAGACGCTTCCTGGCCCACGATGAAACCTGAGAG GGGTGTCCCCTTGCCCCATCCTCTGCCCTAACTACAGTCGTTTACCTGGTGCTGCGTCTTGCTTTTGGTT TCCAGCTGGAGAAAAGAAGACAAGAAAGTCTTGGGCATGAAGGAGCTTTTTGCATCTAGTGGGTGGGAGG GGTCAGGTGTGGGACATGGGAGCAGGAGACTCCACTTTCTTCCTTTGTACAGTAACTTTCAACCTTTTCG TTGGCATGTGTGTTAATCCCTGATCCAAAAAGAACAAATACACGTATGTTATAACCATCAGCCCGCCAGG GTCAGGGAAAGGACTCACCTGACTTTGGACAGCTGGCCTGGGCTCCCCCTGCTCAAACACAGTGGGGATC AGAGAAAAGGGGCTGGAAAGGGGGGAATGGCCCACATCTCAAGAAGCAAGATATTGTTTGTGGTGGTTGT GTGTGGGTGTGTGTTTTTTCTTTTTCTTTCTTTTTATTTTTTTTGAATGGGGGAGGCTATTTATTGTACT GAGAGTGGTGTCTGGATATATTCCTTTTGTCTTCATCACTTTCTGAAAATAAACATAAAACTGT >gi|7019549|ref|NP_037483.1| T-box transcription factor TBX21 [Homo sapiens] MGIVEPGCGDMLTGTEPMPGSDEGRAPGADPQHRYFYPEPGAQDADERRGGGSLGSPYPGGALVPAPPSR FLGAYAYPPRPQAAGFPGAGESFPPPADAEGYQPGEGYAAPDPRAGLYPGPREDYALPAGLEVSGKLRVA LNNHLLWSKFNQHQTEMIITKQGRRMFPFLSFTVAGLEPTSHYRMFVDVVLVDQHHWRYQSGKWVQCGKA EGSMPGNRLYVHPDSPNTGAHWMRQEVSFGKLKLTNNKGASNNVTQMIVLQSLHKYQPRLHIVEVNDGEP EAACNASNTHIFTFQETQFIAVTAYQNAEITQLKIDNNPFAKGFRENFESMYTSVDTSIPSPPGPNCQFL GGDHYSPLLPNQYPVPSRFYPDLPGQAKDVVPQAYWLGAPRDHSYEAEFRAVSMKPAFLPSAPGPTMSYY RGQEVLAPGAGWPVAPQYPPKMGPASWFRPMRTLPMEPGPGGSEGRGPEDQGPPLVWTEIAPIRPESSDS GLGEGDSKRRRVSPYPSSGDSSSPAGAPSPFDKEAEGQFYNYFPN Vaximmutor TLR1 Homo sapiens 7096 41350337 AC108044 NP_003254 9606 4 38797875 38806411 - toll-like receptor 1 7.07 86022.3 786 Also known as TIL; CD281; rsc786; TIL. LPRS5 >gi|224589816:38797875-38806411 Homo sapiens chromosome 4, GRCh37.p5 Primary Assembly CTTATGTGTCAGAACTGTTTAAATCAAAGTTATATCATTTCATTAATTTTTAATACCACATATAAATGGT GAACTGCGACCCGAAGGTATATATTTTTACCTACATCATACACTCACAATTGTGTTTACATCTATGCTGA TGCAAAATAAAGTCATTGTTGGAACTTCCAAAAGCAGCAATATCAACAGGAGGAATATTTTTCACTTGAT GTGTAATCTATTTCTTTGCTTGCTCTGTCAGCTTAATATTAATGGCTGCCCTTAAGTTAGCCCAAAAAAG GCCACGTTTGCTCTTTTCCTTGGGCCATTCCAAATAAGTCCTCCTGGCCATGAGACTTTTGAGCTTGTGA TAACTGCTAGGAATGGAGTACTGCGGAATGGGTTCCAGCAAGATCAGGATTAAGCTATTAGATCCTTCAT GAAAGAGATTGTGATGGGCAAAGTAGAGTTCATAATGGCACCATTCACTCTGGACAAAGTTGGGAGACAA AACAAAGATGGACTTGTAACTCTTCTCAATGCAGGTGATGATATTTTCCACAATGCTCTTGCCAGGAACA AAGTTTCTCTCATGAAGGCAAATCTGCATACCTTCTTTCTCTAGGTTTGGCAATAATTCATTCTTCACCC AGAAAGAATCGTGCCCACTATATGAAATAAATGCATGAAACTGGAGATTTCTTTGGAGTTCTTCTAAGGG TATGTTCCTGGCCCTGCGCCGGGTCTGGGTCCACTGGCACACCATCCTGAGATACCAGGGCAGATCCAAG TAGCTGCAGAGGGAGGTCACAGTCACAGCCAACACCAGCATGGTGGCAACGATGGTGACGATCAGCAGAG TTATGTTGCAGGATAATTCAGACATGTGAAAGTCCTTTAGTAGGGTTCCTCTATAACTTTCCGGGTAGTC ACACTTATAAGAATCAGGCCAGCCCTCTAACACTTCACTTGATACTTGGTCTATATTTTTGACAAATTCT CCTAGCTCACAGGTACATTGGAATGGATTGTCCCCTGCTTTTATTGACCTCATCTTCTGGCAGCTCTGGA AGAAATCAGCCGATGGGTGGGAAACTGAATTGTGATCAATGATCAATACAGAAAGGCTGCTAAAGCTGCC ACATCCAGGAAGGTCAGTTAAAGAATTGAAAGCAACATTGAGTTCTTGCAAAGCTTCCAGTTTTACGACT TGTTTAGGAATGCTCTTTATTTTATTGCTGTGAAGATCAAGTACCTTGATCCTGGGAGGTAAACATCTGA AAATAGTGTCAGTAAGTATATTTGAAGACATATTTAAACTTAATAAACTTTTAGTCCAAGAACAGTCTCC TTTCTTTTCATCATAGCTTACAGAATTCTGGCTAATATCCAATTGTTGCAGAGACTTCATCTGTGTAGTC ATTTCAGCTATTTTTGAAAGTTCTTTTAATTGATTCATTTGTAAAATAAGTGTCTCCAACTCAGTAAGGT GCCCACAATTTTCAAAAACCGTGTCTGTTAAGAGATTATTGGAAAAATCCAAATGCAGGAACGGGCTAAT TTTGGATGGGCAAAGCATGTGGACCATGCGTGTACCAGACACTGTGAAATTTTTGATGTTCATATTCGAA AAGATTTCATAGATATAACTTTGCGGAAAACCGAACACATCGCTGACAACTTGGTGTATAGACAAGGCCT TCAAGGAAGTGCCAGAATAATCAAAATCTCTGAAGTCCAGCTGACCCTGTAGCTTCACGTTTGAAATTGA GAAATACCATACAGTTGTATGCCAAACCAGCTGGAGGATCCTAATGAAAGAATTCCAAGTTGTTTCAATG TTGTTTAAGGTAAGATTTGATAACTTTGGATTTGTTTGAAGTTTCGCCAGAATACTTAGGAAGTAAGAAC ATTTGTTATCTTCTAGCACACATTTGATATTAGATAGTTCCAGATTTGCTACAGTCTTGACTGACACATC CAAAATAAAATGGAATTCTTTGTTTGTGGGGAACACAATGTGCAGACTCTCAGTGTTAAAGTCTTGAAGG CCCTCAGGGTCTTCTTTTTCCCCATAAGTCTCTCCTAAGACCAGCAAGACCTTGCTGATATTCAAATGAG CAATTGGCAGCACACTAGATTTTTCTAAGTGTGTGGTGCTCAACCCCAGAAATTTTAGTTGAGACATATT GCCAAACTCTTTGCATATAGGCAGGGCATCAAATGCATTAAATGACAGGTCCAAGTGCTTGAGGTTCACA GTAGGGTGGCAAGAAATCTTCACCAACTTGTTGTGGGACAAATCCAAGTATTCCAATTCCTGGTTGAATT TGAAAACACTGATATCAAGATACTGGATTCTATTATGAGAAATTATCAAAATCCTCAGTTTTGACAGTGA TAAGATGTCAGAAGTCCAAAGCTCAGATATATAATTTTGCGATATATTTAAGATTGTTGTTTTCTGGGAT AGGTCTTTAGGAACGTGGATGAGACCGTTTTTTGACCTATCAACTAAAAATTCACTTTCTTCAGATAATT GTATTCTGATCTGAAGTATTAACATGAAGATAATGGCAAAATGGAAGATGCTAGTCATTTTGGAACACTA GACATTCCTAAAGGTAGAAGCTGTTCTTCAGATCATCTTGATACAGATACAGATTCTAGAAAAAAAATAA TGAAATGATGAAATACATTACATACATTTTTAAAATACACGAAATTAGTCATGGCTGACATTAAACTTTT TTTTGTTACAGACTTATAAAGTGGAGGCTACATTCTTTTGGGTTACATGGCCTAAAACTAGAGAAGTATA TATATTGGTAAATAATGATGCCAGCTCTAATCAAATAAGTTTTAATATGGATGGTGTAATAGGTTGAATG GTGGCTCACCAAAAGATATGTCTACATCTTAACCATGTCCTAATCCCCAGAAACCATGAATGTTACCTTC CTTAGAAAAGGGGTCTTTGCAGAGATAATAAAGTTAAGGATCTTGAGATGAGGAGATCATCCCAAATTAT CCAGGTGGTCCATAAATCCAATGACAAGTGCTTTTTTAAGAGAAAGGCAGAGGGATATTTGACAAAGAGA CAAGAGAAGAGGCAATTGAGACACAGAGGAGAAGGCCTTGTGACAGTGGAGGCAGAGACTGGAGTGCAGC AGCTACAAGGCAAGGAACATCAACAGGCGCCAGAAGCTAGAGGAGGCTCTGGAGTGATGGTGGCCCTGCT GACACCTTGAGTTTCGACTTCTGCTCTCCAGAACGTTGAGGGCAAAGCCACCACGTTTATGATCATCTGT TACAGCAGCCTCAGGAAACTAATACAGGTGGGTTCATAGAAAGTTTTCTGGCCCCAAGCTTCCCAAAATT ATTTAGAACTCCAGCTAAGACTCAACTGGTGTACTTTCACTCTTTGACGTACATCTCCCCATGACTTTCT CAAGAGGTGTTCCTAATGGTGGTATTGGGACTGCTCTTCTATTACCCTTTATGACCTATACACGGTTTGG GTCTAACTTTGGTCTTACTCAAAAGGTAATTAGATCCTGATAAAAGGCCTCTCAGAAGATCCATTTATTC ATCCATTTATTTATTCAACCAGTATTTATTGATCACCTACTGGCCATGTTTTAAGAACTGGAGATAGAGC AGTGAACAAGATAAAGTCCGAATTCTCAAGGAGTTTTTATCTAGTGGGGAGACAGAAAATAAATGAATGA CTAAACTCAAGTTCCTGTAGTGATAAGTTCTGTAGAGAAAAATAAAGCAGGGTAAATTGTAGGTATGAGA CAGTGCTTGGGGGTGGCTATTTTTGAGTGATTAGTTAGAGAAGGCCTTTTTGAGATGATGACACTAGAGT AGAAATCAAAATGAAGTGAATGAGTAACCTATGGGAAGATCTGGGTAACCTACAGCATTTCTGGCAAAAG AAAAGGCAAGTATAAAGGACCTGAGATGGAATTGAGCTTGGCTTGTCTGAGAAGGAACGGGAAGGTCAAT GTTAGTAAGGAGTTAGGGAAGATGAGAGGAGTTAGGGCCAAGATCACTCAGGCTTTGTTGATCATTGCAA GGAGTTGGGATTCTATTCCAAAAATGATGAGAGGGTGGAGAACACTGGGAGGTTTTGAGAAAGGGAGTTG TTTCCTCCTCTCTCTTATTCAGAATACTTTTTTCATTCATTCATCAAATATGTATTTATTCTCCATGCAG TGGTAGGCACTGTTCTAGGTTATGCCTATTGTACCAGTATTCAAACTGCTCTAGCTAAGACACCATTTTG TGGTCAGCATTCCAGTGGCGTATGAGAACAGAGGGAGCTGTGTTGGGATGAACTCTAGAGTGTGCAGTCC TCACCTCCAGAGACCCTTTCAGCCGTGATTACACAGCTACCCAGTTCTCACAAGGAACTTATTCAAAATC CCCTAGAACCATTAGACAAGCAGGGCATTAGTATCAGCAAAGCACCAAATTCCTCTTCACATATCCCCTC CCATATCTCAATGAGCACAGAGAACCTAAATAATTTAGCTGCTACAAACAACCCTCGGTTACAAAAGTTC CCCAATTTAGATGTGCAAACTTATCTGGGTTTTGGAGCCTTCCTTGGACTTACCCTTTTGTAGGGGTGCC CAATATGCCTTTGTTATCCTGATTTCTTCTAACGAGGAAGAGGGCCTGGTACCCCTATTAGTGTTCATGA AGACCCTGAGCAAGGAAGATGATGCAGACATTAATGTTCATGTTAAAAGTCAAGGTGGTGTTTTTATGAC ATTAACTGTATTTGGCTGAAAGACCCGGAAATTTCCCAATTCTTCCTCTCCAGCTTCCTTAAAGAGCTAC TCACACAAGGAGCAATATTTCAATGGATGTTATAGCTTGAATGTTTGTGCCCTGTGAAATTCTTATGTTG AAATCCTAACCCCCAAGGTGATGGTATTAGCAGGAGAAAATTGTGAAGTAATTAGGTCATAAGAATAGAG CCTTTATGAGTCAGATTAGTACCTTTATAAAAAAGACCCCAGAGAGCCTCAGGCTGTCATTCTGCCATAT GAAAATACAAGAAGGAGGCATCCGCAAACCAGAAGAGGGCCTTCGCCAGAACCCAACACTACCACCCTGA TCTTAGACTTCTACCTTCCAGAACTGTGAAAAGTAAGTGTCTGTTGTTTATAAGTCACCCATTCTGGTAC TTTGTTACAGCAATTTAAACAGACTAAAATAATGGGAATAAAAATAATACCCTGGGGCAGTGCCAAGCTA GGTCGAAGCAAAAGTGGCCTGGTCTTAAGGCCTAAGTGGGGTTCCCTGCTTATTCCTATGCCTGAATCCA GATCAAGGCTCTTTCCACCTCCTAGCCAACTCCAGAGTTCATGGAAGCAGTGATCAGCACAGTCAGGCGT GGTATTCAAAAGTGATCAGAGGCACACAGGACCCCGTGCATGAGTGCGAGAGCTTGGCTCGCAGAACCTA CCACACGCAAGATGAAAGTTGTCCTTTTTCCCTTAACAACAGCTATCATCACAAAAATATATAAATTTAT ATAACTGCCTGCCCCCAAGTGTTAGAGTAGGTACTTAGGCAGACATGAGCAGGACAGGAGAGGGGCCCCC CAACCAGGAATATCAGGTGACCATCAGGTGATGATCAGGCAGTTGTTAAACAGTCTCTCTAAGCCGGGGG CCATGGCTCACGCCTGTAATTCCAGCACTTCGGGAGGCTGAGATAGGTGGATCACTTGAGGTCGGGAGTT TGAGACTAGTCTGGCCAGCATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCGGGCGTGATG GCAGGTGCCTGTAATCCCAGCTACTGGGGAAGCTGAGGCAGGAGAATTGCTTGAACCCAGGAGGCAGAGG TTGCAGTGAGCCAAGATCATGCCATTGCACTCCAGCCTGGGCAACAGGGCTAGACTCTGTCTCAAAAACA AACAAACAAAAACAAAAAAACAGTCTCTCTAGAATAATAATTGTTTGCAGCCAGCATCAGGGAAAGTCAG TCTCCTAATAGATAGAAAACACACCTGAAGCTGGTGGTTAGCAGACTCCTGACAAACTCTCAGGAGCTGG GCAAGCAGGCTTGAGCATGCATACCAAGAGGCAAAATGGCGGACTTCAACGGGTATGTGACCTTCCTCTA GGAAGACTTGTCTGGCAAGGGAAAAATGTCTCCAATGAGCATGTACACAACTTCAGTAAACACAGTGTGT GTGCAGCCCATCCCAAGGGCTGGCAGGCCACTGCACATGCAGACAGCCCACCCCAAGGGAAGAATCAGGG GAGAAGAGACGCAAGACTCCAGAAGCCTGCCAACAAATAAGCCCCAAGTAATAGGTTAAACTGGGCACTT GGATCTCTCAAGTTGCCCACTTGGCCCTCCTCCAAGTGTACTTTATTTCCTTTCGTTCCTGATCTAAAAC TTCTTAATAAACTCTCTGTAAGGAATATGGCTGTGCTTTGGTCAAGGATAGGCCAAGGTAAACATCCAGA GTGACTCAGCGAGTTTAGAGCGCAGGCGTATAACTCACTTTTTATCATAGCCATGTAGACATAACATAGA GAAGCTTACCATAGCCATAACATAGGGAAGGTTCATCCCTTGGCTCTAAGCCACTATTGTCCGTAAATGG TATAATTGCCCTGCTGACGCTGTACAGGTGCATTGGCAGCCAGAGAAAGAGAGAGAGAGAGAGCCAAAGC TGTCCATCTTTGCAGATGGACTGAGGGGAGCCAGGACACAGCTCGGCTTGCTTGTACCCAGAGAGAGAAA GAGTTAAGCTGCTGACCCTGAGGGCAAGGGAGAGCCGGACGCACAGTTGTGTGTGGAAGCTGCCGAACTA AGCAGCCAGGACAAGGCGGACAGTCTGAGAGAACTACTGTAAGTAAGCTGCTAATGAGAGCTGTTGCTGA ATAAAATCACCTTTCACCTGCCTACGGCCCCCTGAGTGTTCTTTCAGCTATCTGCTCATCCACCCCCTCC CCTCAGACCTCAGTATGGGCTGGAACCTGGCCCTGGGCGTGACACTCTCTCTCTTACTCTAAAACTTGCC TCGGTCTCTCACTCTGCCTTATGCCCCTCACACAAATTCTTTCCACTGAGGAGGCAAGAATTAAGTTTCT ACGACCCATATGGATTCACCACTGCTAACGCAAGCATTTAATGAAAGCATAGCTTCAGTTTTATGTCGAG CTAAATTAATTGACTCAAATATCTTCTATTTGCCTAACCTCCATCTTGCCCTTTCATTTAAAGTCATTAA TTTTAAGTACTCAGTTTATGAAACCATTCTTATTTCTATAATTAAGAAATCAAAATGTTATACACAGGAA TAACTGATTTTTAATACTTCCAGGATCTTTGAATTTTATATACCAAACTTGCTAAATCCATGAAAATCAA TGTCTCAGTTTCCTAGACCAAAGAATTTTTTGATCAACGTCTTAATGACCATGACTGAGCCTCATCTATT TTAGAACCTTTAAACTCTTTTACTATCCAAAAAATAAGCTGAGCAGAAAAGGATGCTTTAATTTGAAAGT GTAAAATGCCTCTAGGTGAGCTTCTGTGAATTTTCATTATTATGATAGGTTTAGCCTCACATTATATGAT ACAATGAATCAATACATTTAATTTGTCTTCTTTTGGAGACTTTGGAGGCATCAAATCAAGGCTTTGACTG GTCTTAATGGACAATACCCATACAATCTATCTGGCCCATTAAGTTTAGTCCTGTAAATATTTACTAAGGG CCTTCTCTGCACAAGACCAAATAGCTATTAACCAAAGGTTAAAAGAATCTGAGACAGTTCAAGAACTGTT CTGCTTTGCTTTCTATGCGGTTTTGTGAACTCTTGCTTTGTTATAAAACAAATTCACTTTCAAGTCTTTT GACCAAATTGGATGTAGCCTGGGAAACATTTTCAAAACCTGCAATTCTACCTCACGTCTAGCTCCCCAGA ACAGTGTTAGTGGCCTGAGAAACAGAAGGACTAGCTAGTGGGAAGGCCCCAGAGAGAAAAACTGAAACAA ACCTGGCCACAAAAACAGAAGAGCTGAACAGCAGCATTGCCTCCGGGAGTAACTGACTTATATCTCATTT TCTTTAACACTGAGAAGAGAATACAAATGGCGGGATTAGGTGAAGAGAAATGCTGTCAAAATCAACAACT AGCAGCTGCTCTGGAAGAAAACCAGAAGACGTGTGTGACCCAAATATTCATTTCCCAACTCAAAAATATT TCCTATAAGCAAATCACACACGAGGTGTTATTCCCAGAGGAGTGTGGGGACTTTCTCTGATTTAGGACTC AGAACTGATACACAAATGCCAGTACAAACTACACAGTAACTATATACATATCAGAAATATAACCCACTTA GAAAAACACCCAGGACTTACACAGATTTTTGAGACTTCTGTACTTAATAACTAGCTATACAATTATTTTC TTTTACAAAAGAAAAGAATGTGTTGCTTACCAAGACAACCTGCTTGTCTGTTCCATTTGGCAGTCTG >gi|41350337|ref|NP_003254.2| toll-like receptor 1 precursor [Homo sapiens] MTSIFHFAIIFMLILQIRIQLSEESEFLVDRSKNGLIHVPKDLSQKTTILNISQNYISELWTSDILSLSK LRILIISHNRIQYLDISVFKFNQELEYLDLSHNKLVKISCHPTVNLKHLDLSFNAFDALPICKEFGNMSQ LKFLGLSTTHLEKSSVLPIAHLNISKVLLVLGETYGEKEDPEGLQDFNTESLHIVFPTNKEFHFILDVSV KTVANLELSNIKCVLEDNKCSYFLSILAKLQTNPKLSNLTLNNIETTWNSFIRILQLVWHTTVWYFSISN VKLQGQLDFRDFDYSGTSLKALSIHQVVSDVFGFPQSYIYEIFSNMNIKNFTVSGTRMVHMLCPSKISPF LHLDFSNNLLTDTVFENCGHLTELETLILQMNQLKELSKIAEMTTQMKSLQQLDISQNSVSYDEKKGDCS WTKSLLSLNMSSNILTDTIFRCLPPRIKVLDLHSNKIKSIPKQVVKLEALQELNVAFNSLTDLPGCGSFS SLSVLIIDHNSVSHPSADFFQSCQKMRSIKAGDNPFQCTCELGEFVKNIDQVSSEVLEGWPDSYKCDYPE SYRGTLLKDFHMSELSCNITLLIVTIVATMLVLAVTVTSLCSYLDLPWYLRMVCQWTQTRRRARNIPLEE LQRNLQFHAFISYSGHDSFWVKNELLPNLEKEGMQICLHERNFVPGKSIVENIITCIEKSYKSIFVLSPN FVQSEWCHYELYFAHHNLFHEGSNSLILILLEPIPQYSIPSSYHKLKSLMARRTYLEWPKEKSKRGLFWA NLRAAINIKLTEQAKK Vaximmutor TNF-alpha Mus musculus 21926 7305585 AB039224 NP_038721 10090 17 35199389 35201995 - tumor necrosis factor 4.75 24010.47 235 Also known as DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; MGC151434 >gi|372099093:35199389-35201995 Mus musculus strain C57BL/6J chromosome 17, GRCm38 C57BL/6J CTTTATTTCTCTCAATGACCCGTAGGGCGATTACAGTCACGGCTCCCGTGGGGAGCAGAGGTTCAGTGAT GTAGCGACAGCCTGGTCACCAAATCAGCGTTATTAAGACAATTGGGTTAGATAAATATTTTGTTTTAAAC ATAAGCAAAAGAGGAGGCAACAAGGTAGAGAGGCCAGGTGGGGACAGCTCAGCTCCGTTTTCACAGAAAA CATGTCTGTCTGAAGACAGCTTCCCACACTGGGTCCTCCAGGACACCCCGGCCTTCCAAATAAATACATT CATAAGCAAATAAATAAATAATAAATAAATAATAAATAATAAGTGCAAATATAAATAGAGGGGGGCTGGC TCTGTGAGGAAGGCTGTGCATTGCACCTCAGGGAAGAATCTGGAAAGGTCTGAAGGTAGGAAGGCCTGAG ATCTTATCCAGCCTCATTCTGAGACAGAGGCAACCTGACCACTCTCCCTTTGCAGAACTCAGGAATGGAC ATTCGAGGCTCCAGTGAATTCGGAAAGCCCATTTGAGTCCTTGATGGTGGTGCATGAGAGGCCCACAGTC CAGGTCACTGTCCCAGCATCTTGTGTTTCTGAGTAGTTGTTGAAAGCTCTGAGCACAGAGTTGGACCCTG AGCCATAATCCCCTTTCTAAGTTAGAAGGATACAGACTGGGGGCTCTGAGGAGTAGACAATAAAGGGGTC AGAGTAAAGGGGTCAGAGTGGGGGCTGGGTAGAGAATGGATGAACACCCATTCCCTTCACAGAGCAATGA CTCCAAAGTAGACCTGCCCGGACTCCGCAAAGTCTAAGTACTTGGGCAGATTGACCTCAGCGCTGAGTTG GTCCCCCTTCTCCAGCTGGAAGACTCCTCCCAGGTATATGGGCTCATACCAGGGTTTGAGCTCAGCCCCC TCAGGGGTGTCCTTGGGGCAGGGGCTCTTGACGGCAGAGAGGAGGTTGACTTTCTCCTGGTATGAGATAG CAAATCGGCTGACGGTGTGGGTGAGGAGCACGTAGTCGGGGCAGCCTTGTCCCTTGAAGAGAACCTGGGA GTAGACAAGGTACAACCCATCGGCTGGCACCACTAGTTGGTTGTCTTTGAGATCCATGCCGTTGGCCAGG AGGGCGTTGGCGCGCTGGCTCAGCCACTCCAGCTGCTCCTCCACTTGGTGGTTTGCTGAGGGGGGGGGGG AGGATTGAGTCAGTGTCACCCTCTTAGTTCACACTCCACATCCTGAGCCTCAGCAGCTACCCACACTTCA CTTCCGGTTCCTGCACCCTCTGTCTTTCCACATCCCATTGGCTATGAGGTCCCGGGTGGCCCCCTGATGC CTTGCTTTTGAGTCACTGCTCTGACTCTCACGTGCTGTCTCTAAGAGCTCTGTCTTTTCTCAGCCTGGCT CGACACCCCTCAACCCGCCCCCCAAAATCATGCCCCTTCATTCTCAAGGCACATGTAAAGAAATCTTACC TACGACGTGGGCTACAGGCTTGTCACTCGAATTTTGAGAAGATGATCCTGGAGGGGAAGAGACAAAGGCA AGGATGAGCCTTTTAGGCTTCCCAGCAAGCATCTATGCACTTAGACCCCTTTCCTCCCAAACCAAAGCTT TAAGTTCTCCCCCCACCCCATCTCATCCCATGCCTAACTGCCCTTCCTCCATCTTAAATTAAGAGAGAGG TGTGGGAACACTTACTGAGTGTGAGGGTCTGGGCCATAGAACTGATGAGAGGGAGGCCATTTGGGAACTT CTGTGTAGGAAAAGGAGGTTAGTTAAGACAGACTCACCCCAAAGGAGAAGCCTCCCGGCTGATTGCCCCG CTTACAGTTCCTCTTTGCCCCACCCCACCCCCCAGCTTTGTGTTTTTCTTCTTCATTCATTCATCTGTCC AACCCACGGCTTCTTTCTGCGGTGCCCTCTGTGCTTGATCTCCCGTTATCTCCCCTTCATCTTCCTCCTT ATCTCTCATGCCTCTCTCATTTCTGTCTCTGAGTTTTATCTCTTGCTTATCCCCTCTTCCCCTGGCCACA TCTTTCCAGATCTCTCCACGTGTGAACACACTTGTTCGTTCATTCATCTCTCTGTGCATCCGACGAAGGA TGTTTAGTCAGCTGGACGCATGGGTCCGAGGTCCTGACTCTGTCCCCTCCACACTCTCCTCCACCTTGCC CTGCCCATTAGCCCACTTCTTTCCCTCACACTGTCCTTCTTGCCCTCCTAACCCGTTTTGCTTGTGAGCG AGAATAAGGGTTGCCCAGACACTCACCTCATCCCTTTGGGGACCGATCACCCCGAAGTTCAGTAGACAGA AGAGCGTGGTGGCCCCTGCCACAAGCAGGAATGAGAAGAGGCTGAGACATAGGCACCGCCTGGAGTTCTG GAAGCCCCCCATCTTTTGGGGGAGTGCCTCTTCTGCCAGTTCCACGTCGCGGATCATGCTTTCTGTGCTC ATGGTGTCTTTTCTGGAGGGAGATGTGGCGCCTTGGGCCAGTGAGTGAAAGGGACAGAACCTGCCTGGTT GGCTGCTTGCTTTTCTGGGAGCTATTTCCAAGATGTTCTGGAGTTTCTGTTCTCCCTCCTGGCTAGTCCC TTGCTGTCCTCGCTGAG >gi|7305585|ref|NP_038721.1| tumor necrosis factor [Mus musculus] MSTESMIRDVELAEEALPQKMGGFQNSRRCLCLSLFSFLLVAGATTLFCLLNFGVIGPQRDEKFPNGLPL ISSMAQTLTLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQ VLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQL SAEVNLPKYLDFAESGQVYFGVIAL Vaximmutor trpD Mycobacterium tuberculosis H37Rv 887681 15609329 Rv2192c BX842579 NP_216708 2BPQ 83332 2455630 2456742 - anthranilate phosphoribosyltransferase 6.4 34267.71 370 Catalyzes the conversion of N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate >gi|57116681:2455630-2456742 Mycobacterium tuberculosis H37Rv, complete genome GTCAGATCTGCCGACCGAACCGCACCCATCGCGCGAGCAACTGTTCGGCCGCACCGGTGTCGATTGCCGC ACTGGCCCGCCGCAACCCCTCCTCCCAGGCCGGCAGCCATTCAGCACGGCTGGATAATCCGGCGTGGGCG ACGATCGCACCGGCGGCGTTGAGCACCACAGCGTCCCGGACCGGGCCCCTGGCACCGCCCAACACCGCGC GCACCGCGGCCGCGTTGGCTTGCGCATCGCCTCCAGCCAGCTGGTCAAGCTGGGCGCGCGCAAACCCGAA TCCGGCGGGATCAAACGTCAACTTATCCACGCTGCCCGCCGCAACGCGCCAGATCGTGCTCGTGGTGGTG GTGGTCAACTCGTCCAGCCCATCGTCGCCGTGTACCACCAGCACACTGGACCGGCGCGCAGCAAACACCC CGGCCATCACTTCGGCGAGGTCGGCGAACGCGCATCCGATCAGTCCAGCCCGGGGCCGGGCCGGATTGGT CAGCGGCCCGAGAAGATTGAACACGGTGGGCACACCGATCTCGCGGCGTACCGCGGCCGCGTGCCGGTAG GAGGGATGGAACCGCGGCGCGAAGCAGAACCCGATCCCAACCTCCGCGAGGCTGCGCGCGACCAGGTCGG GTCCCAGGTCGATGCGCACCCCCAGCGCCTCCAGCGTGTCGGCGCCACCGGACAACGAGGACGCCGCTCG GTTGCCGTGCTTGACCACCGGCACACCCGCAGCCGCCACCACAATCGCCGCCATGGTGGATAGGTTCACC GTGTTGACTCCGTCGCCACCGGTGCCGACGACGTCGACGGCGTCGTCGGGGACCGTATCGGCGGGCAACG GATGCGCGTGGCTGAGCATGACGCCAGCGAGCTCACCGACTTCGTCGGCGGTCGGAGCCTTCATCGTCAT CGCCACCGCGAAGGCGGCGATCTGCGCCGGCCGCGCATTGCCGGTCATGATCTGGTCCATGGCCCAGGCA GCCTGGCCCCGCGCCAGATCGCGGTTGTCGGTCAACCGCCCCAAAATCTGCGGCCAGGACGGCACCGATG CGGCTTCTGCTTTCGGCGAGCCCCCGCGAGATCCCCCCGAAGAACCCTCAGCTGACAGCGCCA >gi|15609329|ref|NP_216708.1| anthranilate phosphoribosyltransferase [Mycobacterium tuberculosis H37Rv] MALSAEGSSGGSRGGSPKAEAASVPSWPQILGRLTDNRDLARGQAAWAMDQIMTGNARPAQIAAFAVAMT MKAPTADEVGELAGVMLSHAHPLPADTVPDDAVDVVGTGGDGVNTVNLSTMAAIVVAAAGVPVVKHGNRA ASSLSGGADTLEALGVRIDLGPDLVARSLAEVGIGFCFAPRFHPSYRHAAAVRREIGVPTVFNLLGPLTN PARPRAGLIGCAFADLAEVMAGVFAARRSSVLVVHGDDGLDELTTTTTSTIWRVAAGSVDKLTFDPAGFG FARAQLDQLAGGDAQANAAAVRAVLGGARGPVRDAVVLNAAGAIVAHAGLSSRAEWLPAWEEGLRRASAA IDTGAAEQLLARWVRFGRQI Virmugen A trpD mutant is highly attenuated in mice and induces more protection from challenge with wild type M. tuberculosis than that induced by the current vaccine BCG [Ref1672:Smith et al., 2001]. TSC22D4 Homo sapiens 81628 13569901 AC092849 NP_112197 9606 7 94697542 94710214 - TSC22 domain family, member 4 7.24 39140.01 395 Also known as THG1; THG-1; TILZ2 >gi|157734172:94697542-94710214 Homo sapiens chromosome 7, alternate assembly HuRef, whole genome shotgun sequence ATTCAAGTTTCTTCAGAGTTTTTAATTATTATTATTATTTTAATAACAACCTGAATCCCAGAACTTCCAG ATACTGTCCACACTCCCTCCCCTGGGCAGAGGAGAGGAGGCACCTCAAGGGAACAAGATGGGGGTGGGGT GTCTGCCGGGGAGCCTCCGACTCCCCCAAGCCGTCTACTGCTGGGGGGTCCCAGGAGGGAGGGTGGGGGT TGGTTCCCTCCCAGAGGGGGCTCCCTCTGACGCCCCGTCCTGAAGCCCTCCCTCCTCCATCAAGGCTGGG GATGATGAGGAGATGGGGCAGGGGCCGGGGGACCAGGCCATTACTGAGCCTTGAACTCCCACCCCGGGGA CACGGGGACATTAAAGCTGCATAGGAAGAGGGGGCAGGCGGCTGACGCAAGGCCGGGCAGCCCCAAAGGC ACATTGTAAGGGAAGGGAGGCTCAGACGGAGGGCCCATTGGGCGCAGGGGGCCCAAGCCGTGGGACCCCC GAGGAGGGCAGCTGAGCCAGCTGCTCCGGGCTGGCCAGGGCGCGCAGCAGCCCATTCTCCTGCTCCAGCG CAGCGTTCCGCTCCGCCAATTCCCGGATCTGCTCCTTCAGCACCTCCACCTCCTCCCGGACCGCAAACAT GAGGTGGGACTTCACCAAGTCCTGGGGGCCCCGGGACAGGCACAGCGTCAACGGGGTGGGTGCCTCATCC CCTGCCCAGTGCCTTGAGGGCTGGGGTGGGAGACAGTCCCCAGTGTCCAGAGGATGAGGGACAAAGCAGG GAAGGGACAGGGGAAAAGGACGAGGGACAGAGGCAGAGTCAGGGCTCAAAGACAGAGATGGGGACCAGTA GCCAGACTAGAGGCTGGGCAAAGTCAAGGGTGGAGGTGGTGGCAGGAGGGGGCAGAGGATGCCCAGCAGC CCAGCAGGGAGAGGGACGGAGGAGCTGGGAGTTGGTGTTCCCTGGTAAGGAAGAGGACAGGGCTGGGGCA GAGGGATGGCTTAAGGGGGCCAGGACCTCCTGGGACCAAGATGGCTTCTCTTACCATGGCTTGCTCGATT TTGTTGTCAATGCCAACCAGGCTTCCGGAGCCACTGGAGAGACACAGGAAGGTGAGGGGAGGAGAGGAGA AGCCTTCCCAGGTGCTGGTTTCTTGGACCACAGCCTCCCGCCCACACCCCCCTGTACCTGTGACAGTAGA GAATGAGGGAGAGGAGGGTTTTGTCCATCCCCCTGCGGGTTGAAGGCCGCTGCCCAGGGCCTCCCAGGCC CCGGTGGGGCGGGGGCCCAGATGTCCCTCGGATGGGGGATGCACCCCGGGGAGAGCAAGGGGAAGAGGCA CAGCCCCTTTTTCGGGGCAGCCCCGGAGCCATCCCCACCACTCGGGTCTCATAAGACGACACCAGGGACT TGACAGAGACCCCTGGCTGGGCCATGCCCAAGGGGAAAGGGTCCTTAGACACCCCCCCAATGGAGAGGGA TGCAGTGAAGGGAATGCCTGGGGGGCTCAGAGCCCCCCGGGCAGAGCAGGGTCTGGCTGGTGCCAGGCAG GCAGAGAGGAGACAGTGCCTCGGGGGTGCTGGCCTGAAAGATATACACCACCCCCCCAAGGGCAAGGCCT GGCTGCTATTGGCTCCTTGGGGACACGGGGACCCCCCCTCCTTTCAGCTCCTCGGTGGGCGGGAATTCCC AGACACGGTCAGTGCCAGTGTCCCCTTCTCACCCTGGCACCAATCCCAGAGTGGAGGGGAGGGGGCCGGC CGAGGAAGTCAGCTGGTGGCTGGGCCAGGGGAGGGGCGTGGGGCAGAAGCCTGGGTCTGAGCGGGGAGTG AAGGGTGTGAAGGGAGAGGAAGGGGCTGGCCTTCGGAGGCCCCTCGCCACCTCCGACCCCATTCTGTCCC CTCCGTCACTCAAGGCCTTGGGAAAGAGGGTGGAGGTGGCTGGGGTAGGGGGCGTTCTGACGTGCTCGTG GTCTTCCATAGATCATGAGCGCTCTCCCACCAAAAAACCCCACAAGGCTCACATCATGGGTGACCCCTGG GATCTGGCCAGAGGGTCCCTGAGTCTCCAGAGAAGACCAAAAATGGGAAAGGTTACCATGGCAACAGAAA CTGAGCCCAAAGCCCTAGAAGATGAATACAGCGTAAAGGCCAGGCGTGGTGGCTCACACCTGTAATCCCA GCACTTTGGGAGGCCAAGGTGGGTGTATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGA GAAACCCCATCTCTACTGAAAATACAAAAATTAGCCAGGCGTGGTGGAGGGTGCCAGCAATTCCAGCTAC TCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCCAGGAGGTGGAGGTTGCAGTGAGCCGAGATCGTGCTA CTGCACTCCAGCCTGGACGACAGAGTGAGATCTTGTCTCAAAAAAAAGCCAGGTGTGGTGGCTCACGCTT GTAATCCCAGCACTTTGGGAGGCTGAGGCGAGTGGATCACCTGAGGTCAGAAGTTTGAGACCAGCCTGGC CAACATGGTGAAACCCTGGCTCTACTAAAAGTACAAAAAATTAGCTGGGCATGGTGGGGGGTGCCTGTAA TCCTAGCTACTCTGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCGA GATCGTGCCACTGAATTCCAGCCTGGACAACAAGAGCAAAACTCTGTCTTAAAAAAAAAAAAAAAAAAAT CGCCTATGGGCCTGGGACCTGGGTCTGGGGTCTCAGAGGAAGCGAGGGTTTGAGGAGAGGAAGGGAAGTG AGCTTGGAGAAGGGACACTGGGAAGAGGAGCAACAGGGCTTGGAAGAGGGCTGGGAGTCTGGGGTGACAG GGTCTGGCTGCAGTCTGGGAAGTGGCCACCAGAGGGCGACTCAGGCCTGGCTCAACAGCCCCCAGGGCCG GGCTCCAGGCTGGCTCCACGACGTTGGGCTCCTGGTCCCAGAACCTGGCTCTCCCTCAAGCCCCACTCTC AGGCCCCACCTGCGCAGGCCCTTGTGCTGACCTCATCCTCCAAGGCTCCCTCTCCCACCCCTCAGGCCCC AGGCTCTTGCCCTGAGGCTCCCGACCCACCCTTGCACCCCTGCACTCTGGCGCTGTGGCGGGATCCCCAG TCCTGCCCTCCAGCCCCCAGCTGGGCCCCCCTTGGAGCAGGGGCCTCAAGGCGCTGCAGCCCACATCCCT GGACTCCCTTGTAAGCCCTCCCTCCTGGCTGTCAGCTCAGACTTGCTTTCCTGCAGGGGGATCAGCACTC AATTCAAGGTGACCCCTGGATAAGGTCCTGCAGCCAGGAAGGGAGCACAAGGGAGCTGGGAATTTTCCAT TTCTCATCCCAGCTCTGGGTGCAGAAGGCGCTGACAGGTGGAGCAACTAAGGAGGTGACTAGGCCTGAGC GAGCAGGATGGGGTGGGTGGGGGAAGGGCATCTGGGACTCTTGGGGTGGTGGGAGAGGAGAAGTTGAAGG GTGGAGGTGGGTCTGCCAGCAGGAGGGTCAAAGTAATTTTCGAGCTATTTCAATCAGCAACACCTGGGTG GAGATGGAGCCACCACAGAAAGCCCAATACTGCCACCACCCCATTAAACACTCACAGAGACCAAAGGGGT TGGATACACAGGGATAGGAACATGGGCCCTGCTGGGCAGAGACTCAGGGGTAAAGATGTCAAGTAAGGTG TTCATTCTCACCAGAGATAAAGGAATAATACAGCAATAAACGTTTGTTAGCCGACAGGTGACTGTCTTCT GTTTGTCTAGCGCCTGGGAGTTTTTGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCGGGATTTTCCCTCA TTGGAACCTCCACCTCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCAGAGTAGCTGAGATTACAGGCA TGAGCCACCATGCCCAGCTAATTTTTGTATTTTTGCCGGGGAGCCTCCGGCAAAATGGGGTTTCACCATG TTGGCCAGGCTGGTCTTGAACTCCTGACCTCATGATCCACCCACCTCAGCCTCCCATCTCAGTCCTGGGA TTACAGGTGTGAGTCACTGTGCCAGGCCTCTATTGGAACATTCTAACACACACTTCAAGGGACCAGGGCT CTGGCAATGCAGGCCCTTCCCTTTAGGACCCCAAAGCGAGGGGTTGTGGGCACTGTGCTAGAATTCTTTG ACAGGGTCCGGGGGAGGAAAGTAGGGGCTGCTGAGACAGGATCGACCTCCACCACAAGCCCAAAAGCAAG TTTGTATGGATTAGAAAATGCCCCCTTCTTCGAGATCCTTAATGCCATCTTCTGGAAAACTGTCGTGACT TCAAGCATTGACTGTGATTTCAAATATGAGCGGCACCCCCTGGAGTTAGTTGTGCAGTGTGTGACATGCA CAGTGGGAGACTTAGCCCTGCAGGACTCTTGGTCCTGGGGCGGGGACAGCCCTAGAAGCTGGTCTTCCTC TTTGAGGGTCAAATAAAACGGCCTCAACATGAAGATGAGATAAAAAGCAGGACACCTGGGAGAAATGGGG AGCCTGGGATCTTGGGGACAGGGGCCAGCCCTGCACACTCTCCAGTGGGTTCAGGGGATCCCCCAGGGCC TAGGTCTGGGGCCAAGAAAGTGTCATCCAGCTGCCCTGTCTCTATGCTGTCCCGGATCTGTGGTGTCTTT GGGGCGGGGCTGGGGAGGTCGGGGAGGGGGAAGGGGGGCTGGCTCCCTGCCCTGCTTGGGAGAGCTGTAC CTGACTGTCCTAGAGAGAAAGGATATTGTAGACCATTGCCGCCACCTCTTTGCCTGGGACACAGGCATCC CAGTAGGACAGTGGAGAAGGTGTGGCTTTGGAGTTGGACCCAGGCTCCAGCTGCAGTCCAGCCACTTAAA CTAGCTGGGTGGCCCTTGGGCAAGTTTCTAAAACCCTCCTGGCCGGGTGAGGTGGCTCATGCTTGTAATC CCAGCACTTTGGGAGGTCGAGGTGGGTGGATCACCTGAGGTCAGGAGTTTGAGATCAGCCTGGCCAACAT GGTGAAACCCCGTTTCTACTAAAAATACAAAAATTAGCCGGGCATGGTGGCATGCACCTGTAGTCCCAGC TACTGGGGAGGCTGAGCCAGGAGAATCGCTTAAACCTGGGAGGCAGAGGTTGCAGTGAGCCAAGATCGTG CCACTGCATTCCAGCTTGGGAAACAGAGCAAGACTCCATCTAAAAAAGAAAAAAACAAAAACAAAACAAA AAAAAACCTCCTTCTCTGAGTCTCAGATACTTCATCTGGCAAGTGGGAGAATGGAACTGCTTTTTAGGAT TAGAAGGGAGAAGGCAGTACCGGACCCAAGGGAGGCACTGGATAAATAAATGTACCTTTTCTTTCCCAAG TCCCTGGGATCCATTCTGTATAATGCCTGGATCCCCCAGGCCGGTCACTGCCCCCACCCCCATTCCTTCC ATCCACTTCTTCACACCTTAATTACCTCCTGCCTGCCGGGAGGGGACAAGGATTTCCAGCCCTGGGGCAC GTGAGCATTCTAGATTCACCTGCACCCTCTGTTCGCCCATCCCCAGATTCAGGGACTGGTCAGGCGTCCC CAACTCCCTCCCTGTGATCCAGGCACTGAACATCTCAGCCCACCTTCCCTCTTAAGAGAGAGGTCCCAGG CCACTGGCATCCTGTCCTGCCAGCCCTGTGCACACCAGACAGGAGAGGTGGGGCCTACATGTCCCATTCC TAGGGGGCAGGGGGTGGCCCAACCCAAACCCGCTGGCCTGGCAGGGTGGGGCTTGAGGGGCTGGGCTGGG ACAGATCCCCAAGAGCCTTGGAGCACAGCAGGGTGAAGTGGAGGCTGGGAACCGGACCGGTCAGATTGGG GTGTGCAGGGAGGGGGTGGGATGGAGGGAGGCAGGGAGGGAGGGTACAGGGGAAAGACGGAGGCCTCAGG AGGTTGAATCCTCTAAGACAGAGACAGCAGGAGGCTGGGCTGTGAACTCAACAGGGGTGGGGGAGGGACG GGGAGTCCTCTTGCCGGGGAACCTCCCAAGTGGGAGAGGGAGAGGGCGGGAAACGCTGAGAATAGCAGTG ATGGCGGCAGGTGACTCTCCCAGCCCTGGCCGACCTCACACCTCGCACATTCCTGAGAGTGGGAATGGGG CGTGCAGAAGCCACCCAGTCCTGCCCAGCCCCGCCCAGCCCCACCCAGCACTGGCACTCTTGCCCTCCGT CCCACCAGCTCCTGGCCACCTCCTTTGCCAGGTCCCTATCCTCCCTGAGGCCGCAGGACCCTATCAGTGG TGGGCTTGAGTCCCTGATCCCAGCGAGTGGCCCCTGATCTGGGGAGGACAGGAAGGAGCGACTCCCATCT GGAATTGGTCCCTCAGGCACCCACTCACCCACTCTCGAAGCCCCACCCCCAATTTCCTTCCAAGCAGGAA GTCCCTCCCCTTGAAAACCTCCCACGGGAAGTCCCACTTCTCCCTCCAAAAGAAAAGCGGTGACTCCCCA GCCCTTCTGCCCGGTTCCTATCAGTCCCCAGGGAGAAGCAGGCAGCCTGGCTATCTCTGACTCACGATTC TGGGCCTCCCTTTTCAGCTTGCTGGCTTTCTTAGGAATGGTGACACCTGGCCCTGAAAACCCACCCAACA GAGCACTGTCCCCTAAAGGGAGAACCCCAGAAAGTCGCTCTCTGCAGTGCTGCCCCTGCCCCTTTGCCGT TCCTAGCTCTGATTCTGTTCCTAGCTGGCCCATCTTAGGGACTTGCTTTCTCGGTTCTAGAAATCTTAGG ATCCTAAAGTAGACCCTGTGGGCTATCAGGATGTCACGCTCCACAGGTGCTGTTAGGGAGTCCCAGTCCT TTGTTAGGAGAGAAAGAGGGAGATGGAACTGGGAGGAAACTGATTTCCATTCTTTTTCAGTTTTTTTTGT TGTTTTTTTTTTGAGGCGGAGTCTCGCTCTGTCACCCACGCTGGAGTGCAGTGGTGCGATCTCGGCTCAC TGCACCCTCTGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCA CGTACCACCACGCCTGGCTAATTTTTGTATTTTTAGTACAGACAGGGTTTCACCGTGTTGGCAAGGCTGG CCTCGAACTCCTGACCTCAGGTGATCTGCCCGCTTCAGCCTCCTAAAGTGCTGGGATTACAGGCATGAGC CACCATGCCCAGCCTACTTCCTTTATGTATATATATATATATTTTTTTTTTTCTTTCTTTCTTTTTCTTT TTAGAGATGAGGTCTCGCTACATTGCCAGGGCTTGGTCTTGAACTCCTGGCCTCAAGCAATTTTCCTGCC TGGGCCTCCCAAAGCACTATGAGTACAGGCGGGAGCCACTGTGCCCCGGCCTGATTTGCTATTCTGTCTA GAGCATGCATTTGCCAGTGGGTTCCTGGGTTGGCCAGATGATCTCTAAATCTGCAAGAGCTCTGGAATCT GACAACAATGACTCCCTTCAGACTCTAAAAAAGTCTGTGACCTGACAGTTCTGAGCCAGGGAGTCCCACC CAGCCCTCTCCACAGAGAGGGAGTGGGTCCGAAATCAACTGTGTGCAGTGGCTATGCCCAGGCCAGGTTT CTCTAAGAGGTCCTCTCCAAGTTCAACCTGGGGGAAGGATGCCTACCAGGCACTTTCTTACAGCTACCCC GGGTGCTGGGCGGGGAACCTGAACCCCACGCCCCACCACCCCACGAAGCCCACCTACCTATCATCGTCGC TGTCTAGGTGACCACTGATGGCCAACATGGAGTGGGCCAGGCTGAACTTCTGAGCTGCTACTGCTCCGAA GGGGTCTGGAGATTTGTGAACCAGGCTGGCATCGTGGGTGAAGTAGAGGGCTGGGGAGCTGGGGCGAGAG TCAAGTGGGGGCACCTGGAGGCGGAGAGGTAGGAACCATCACATGAAAAGAGAAAAGAAAGGAACCAGCT GCCTTAAAACTTGCCTATTAGCCTTCCTCCCTCTCCACCTCCCCACTCTCTTTCGAGGACCCAGGAGTCC TGTCCCCGCAGTCCTTCCCTCCTCCAGCTCTGGAGAAGGCATTAGGCAGGTACTAGAAGCAAGAAGTGGG TCAACCTAAGGAATGGGAGGAGTGGTGTATAGGACATTAGAGAGAGTCTCCCAAGTGGGAGGAGGGGGTG ACTGACGGAGGAACCCAGAATAGACTCCAATGCCCCAGCTCCCACTGGGCCGGGACCCCTAATCAGAGGC TACGCAGCGTGTCTGCCTTCTTTTATGTTTTATTTTTATTTTTTTGAGACAGTCTCACTCTGTCACCCAG GCTGGAGTGAAGTGGCACAATCATGGCTCACTGCAGCCTTGAATTCCTGAGCTTAAGCGATCCTCCAGCC TCAGCCACCCGAGTAGCTGGGACTACAGGCATGCACCATCATAACTGGCTAATTTTTTATTTTTGGTTAA CACAGGGTCTCAAGATGTTGTCCCGGCTGGTCTCAAACTCCTGGGCTCAAGTGATCCTCCTGCCTCAGCC TCCCAAAGTGCTGGAAGAATGAGCCACCACTCCCAGCCTGTGCCTTTACTGGAGTCTCGCTCTGTCGCCC ATGCTGGAGTGCAGTGACGTGAACTTGGCTCACTGTAACCTATGCCTTCCAGGTTAAACTGATTCTCCTG CCTCAGCCTCCTGAGTAGCCGGGACTACCGGCACCTGCCATCACACCTGGCTAATTTTTTTATTTTTAGT AGAGACGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCTCAAGTGATCTGCCCGCC TCGGCCTCCCAAAATGCTGGGATTACAGGCGTGAACCACTGCACCCAGGCTGCCTTCTTAAAATGACTCC AATGTCACCTCTTAGAGAGGCATTTCCCCATCATTCACTGGTTTACTGAACTATTTCATTTTTTTATAGC ACCTAACATTATCTGAAATTAAAGTATTTATTGTGTCTCTCCCCTGCTAGAAGGTCAGTTTGCTCAGTAG CTATTTGTAGAATGAATGGATCCTCAGAAACTGCTCCTCCATCCCCTCACCCACCCCCCACTCTCGCTTC CCCTCTCAAACTTCTCGGACTCAGGTGGAATTCTGCTCCTGCGCTCTGCCCTAGGGTTGGGGGGGGAGCT GGTTGTGGAGCTGGGGGGAGGCGGAGCCTGGGCGTGGACCTGCATAGCCCAACAGGCTCTGGGGGCAGCA GCAAAAAGGGCGGGGGCGACAGGAACATGACAAGACGCCTCTGTGAGGCCCCGGGAGGCCCCAGCTTCTC AGAAAAGCCAAGAACAGAGAGTCCAGGGTGGTTAAGGGACAGAGCAGAGGAAGGGGGCCCAGGTGGTGTC CCCTGGCCCTTCTCCACCACTCCCCTCTGTTAAGAGGGAGAAGAGGCATGAGGGGACGTGGAAGGGAGAC AGACTTTTGGGCAAGGAAATAGCAAAACAGATGCGTGCAAACAGAAGACCGTCAGGAGGTGGGGGAAGGC TGGGGAGTAGGGGGCACTGAGAGAGAGAGGCAGGGTCTGTGGCCCCAAGAGAAGCTCAGGCCAGGCTTAA GGACAGAGAGCAGGGAAGCTGGAGGCCAAGGGGGTTTGCTTTGGAAGAGTCTGGGGACAGGACAGGAAGA GAAACAGCATCTTCTCCCCTGGGGTGGGTGCTGAATGCAAGAGTTCAAGAAGAGTTGGGGGAGGGGGGGT AGGAGGGGGCATCACGAGAGATGTGAGCTTGAGGCCAGGAGGGGTTTGGGGGGAGCCAGTCCTGACCGGT CCTGACAGGACCCAGACATCCAGTCCCAGGTTATTTTTATACTGTGGTTTGTGCCCAAGGCCAGGAAGGG GAAAGAGTTCGCATCCTCACACTCCCTGCCTTTCCCCAGACCTCCGAGGTGTTATATTCTCCCACAGGAC AGAAGTATCCAACCTTCTATCTCTGGCTGCTCACCCCCACCATGGGGACCCAGGCATCCTGCCCCCACAG CCTGGCTTGTTCCTCTTTTGGAGACAGGAAGGAGAAAGAGTATGGGACTGAACTCCCTTCTGGGGCTCTT CTCTCCCACTCCACAGTGGTCACCCTCGAGTTATCAATCACAGAGAGGCTCAGATGGACGGGGGTGAGCC AGACAACTGGGGGGTGAAATGGATCAACCAGCAGGCCCCCTCCCTGTCCCTGAGAGGTGTTCCCCCCACC CAATTTCTCAGTGACACTTGGCTTACAGCAAAGCTGGAGGCAGGACTGAGATGGGTGGCTGGGGGAAAAG AAAGACCCCCACATTCAGGATGGGGCTGCAGTGCAGAGACTGGCAGCGCGCAAGGCCTACCTTTTACTTT TCCTCCCCAAATCCTGGCAGCTCCTCCCCTAGGGACCCAGCTACCCAGCTATCCAGCTGGGGCCTCCACC CTCATCACCTAGAGGCAGAGGGGCTGAGACCAAGACAGAAGAAGGCCTGATGAGGGGGCCACATATGCTA CAGGCTACATGAAGACCAAGACAGAGAATCAGAGGCCAGGGAGAAAGAGAGAGGGTGCAGAAAAACAGGA AGGAGGCTGAGACAGAAAATGAAGTAGGAGGAAGGGGCTTCAGAGTGACCTGGCAGGCACAGAGAAGAGG GCTATCTGATCTTATAAAGTGATGGAGAAGGAGGAGGAGAGGGGGGGGAGGAGGAGGAAGGAGGCTCAAG ATAGAGGTTAGGCCAGGGCTGATGGGCCAGCCCTAGAACCCAGGTCTTACCTGCCCCATCTCTTCTGGAG CACCCAACTCCATCCGCAGCCGCATGTCTACAGCTTTCCTCCGGGACAGTGGAGGGGTCCTGGCCCCTGA GCCCCCAGCCTCCGCTTCCACCCTCAGAGAGGGCAGGGGCGTGGCAGCCCGGGATGTGCCCGCACTCTCA CCGGTCTCAGGCTCTGGGGGGCGCTGCTGGGGTGAGGAGGCCGACAGTGGGGGTTTCTCTGCCTTGGCTT TGCTGGGCACCACCAGCTGGCCCAGTCCCCCAGTGAAGGAGCGGGCCTGAGGTCCAGGAGAGGTGGGGGG TGGACGGAGCCAGGAGGTGGGGCCCTGAGACAGGCCTGACGGTGGGGTGGGGGCGCCCAGGCCGAGGCTG GCCAGCTCCAACCTGGAATCCAAAGATCTGCCCCCGGCGCCCCCTGAGGCCCCTCGAATTCCCTCCAGGA GTCCGCCGAAGCTGTGGGGCTCCAGGTCTCGCTCATAAACATCCACACACGTCCAGCGACCGCGGCGATA AGGCTCTCCCAGGCCGTGGGGCAGCTTCACCACCCGGAAACGGGAGGAAGGGGCCCCAGGTGGTGGGGAG CCATTCCGGGGGGTGCCCTTGCCCCCCGGATCGGGGCTGGGCTCCCCATTGGGCAGGCGGGGCGGGGGCC CGGTTGGGGGCTGTGGGGTAGGGGGATCCGAAGCCCCTGGGCTCCCAGGGCCCTCATAGTCCGTGGTGAC GCTGGTGATTTGGAAACTACTCTTCTTCTTGCCCCCGCTCATGGTCCCTGGGGCTCAGGGCTGGGCCAAG GTTGGGGGTGGGTTGGGGCTCCTTGAAGGGGCTCAGGCACCCCTGGAACAAGGGGGCCACATGGCGGGGA CATCCGAGCCCCTGGGGACGTCTGGGTCCGACATGGCAGGAGGGCCTGGCGGGAACCGGGGGTGCCTGCT GGGTGAGGGGAGAAGAGGAGAGGGGAGGTGGCGGGAGGGGGGAGCAGGGGCAGGTTTTTCCTGCTGCTGT TGCTGCTGAACTCCAGAGCTGAGTTTGCAAACTGGAAAAAGAGGGGGAGAGAGAGAGAGAGAGAGAGAGA GAGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGAAACCAGTGGGCAGG CCACCTCCGGGGCTTCCTCTCCGGGGAGCAGAGTGGGGGAAGGGGAAACCGTCAGAGACCCAGAGACAGG CAAGAGATGACAGAGACCAGGGCAGAGGGGACTGGATCCAAGAAGGCACTCAGAGTAGAAAGAGGCAGAT GAGTCCCAGAGTTGGGCATTTCTCGCCTGGGATTCTGACTCAGGGGGCCCGTATTGTCCCAAGAGCTCGG ACTCCAGGGCCTGGGAGAACCAGGCTGAAGCAGCTCCCCAGCCTGGCCCGGCCACTCAGAGAGACCTCTC CTGGGCCTCGAGCCCCCACCCTCACCCCAGCTCCCACATCTCTGGGGCAGGCCCTGCCCGGCTACCCCCC AGCGCCCCACCCAGCCCTGAACCCTACCTCTGGGGCCTCGGCCACTGATCTGAGCCTGAGGGTCCCGGCG CCTAAGAGGTCGGGCTGTCCGTTCCCTGGGGCCTCCTGGCCATGGGCAGTGGCGGGGCACGCAGGCGGCG ATCAGAGGCTGTCCCGTCCTCTCCGGGGGCCGCGGCTCATCCTGCCAGGCATCTCCGAGGAAAGTTTGCT CTCCGGAAAAGAAGAAACCCGCGCCTCCTCCGAGGAGCCTAGGAGCGGCCTCTGAAGCCCCAAGGGTCTG GAGACTGATGTGGGAGGGGGCGGCCGAGCCCCCCTCTGCTCCCGGTGGCAGCCAGCGAGTGGGTGTCCCG AGTCTCCTCCCGCGTGACCCTGCTGGGTCCGTGTCTCCGCTCCTCGGCTCCCCTGGGTCTCTGCCTCTCC GTC >gi|13569901|ref|NP_112197.1| TSC22 domain family protein 4 [Homo sapiens] MSGGKKKSSFQITSVTTDYEGPGSPGASDPPTPQPPTGPPPRLPNGEPSPDPGGKGTPRNGSPPPGAPSS RFRVVKLPHGLGEPYRRGRWTCVDVYERDLEPHSFGGLLEGIRGASGGAGGRSLDSRLELASLGLGAPTP PSGLSQGPTSWLRPPPTSPGPQARSFTGGLGQLVVPSKAKAEKPPLSASSPQQRPPEPETGESAGTSRAA TPLPSLRVEAEAGGSGARTPPLSRRKAVDMRLRMELGAPEEMGQVPPLDSRPSSPALYFTHDASLVHKSP DPFGAVAAQKFSLAHSMLAISGHLDSDDDSGSGSLVGIDNKIEQAMDLVKSHLMFAVREEVEVLKEQIRE LAERNAALEQENGLLRALASPEQLAQLPSSGVPRLGPPAPNGPSV Vaximmutor Aagaard et al., 2011 journal Aagaard C, Hoang T, Dietrich J, Cardona PJ, Izzo A, Dolganov G, Schoolnik GK, Cassidy JP, Billeskov R, Andersen P 2011 17 2 189-194 Nature medicine Agger et al., 2002 journal Agger EM, Andersen P 2002 Nov 22 21 1-2 7-14 Vaccine Aguilar et al., 2006 journal Aguilar 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