Schistosoma japonicum 6182 Schistosomiasis Schistosoma japonica remains endemic in seven provinces in China. Human infection is acquired during the course of domestic or occupational activities, in particular fishing and farming. Acute infection may result in fever, weakness, diarrhoea, abdominal pain and hepatomegaly. Chronic disease involves granuloma formation, tissue inflammation, liver lesions and fibrosis, which may persist after infection has been cleared. Schistosoma japonicum is also known to infect 45 species of animals, of which water buffaloes are especially important for transmission (Balen et al., 2007). Baboon Papio cynocephalus 9556 Bank vole Clethrionomys glareolus 447135 Bear Ursus americanus 9643 Birds Passeroidea 175121 Brown Trout Salmo trutta 8032 Buffalo Bison bison 9901 Carnivores Vulpes 9625 Cat Felis catus 9685 Catfishes Siluriformes 7995 Cattle Bos taurus 9913 Chicken Gallus gallus 9031 Chimpanzee Pan troglodytes 9598 chinchillas Chinchillidae 10150 Copper Pheasant Syrmaticus soemmerringii 9067 Deer Cervus elaphus 9860 Deer mouse Peromyscus maniculatus 10042 Dog Canis familiaris 9615 Ducks Anas 8835 Ferret Mustela putorius furo 9669 Fish Hyperotreti 117565 Gerbil Gerbillina 10045 Goat Capra hircus 9925 Gray wolf Canis lupus 9612 Guinea pig Cavia porcellus 10141 Hamster Mesocricetus auratus 10036 Horse Equus caballus 9796 Human Homo sapiens 9606 Macaque Macaca fascicularis 9541 Mongolian Gerbil Meriones unguiculatus 10047 Monkey Platyrrhini 9479 Mouse Mus musculus 10090 None None Parrot Psittacidae 9224 Pig Sus scrofa 9823 Rabbit Oryctolagus cuniculus 9986 Rainbow trout Oncorhynchus mykiss 8022 Rat Rattus 10114 Raven Corvus corax 56781 sei whale Balaenoptera borealis 9768 Sheep Ovis aries 9940 Squirrel Spermophilus richardsonii 37591 Tree shrew Tupaiidae 9393 Trouts, salmons & chars Salmoninae 504568 Turkey Meleagris gallopavo 9103 Vole Microtus ochrogaster 79684 Water buffalo Bubalus bubalis 391902 Chinese S. japonicum DNA vaccine pCMV-Sjc97 encoding paramyosin VO_0004456 DNA vaccine Research pcDNA1/Amp [Ref2391:Zhou et al., 2000] Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction Vector pcDNA vector/Amp expressed the full-length cDNA encoding paramyosin of Chinese S. japonicum (Sjc97) (Zhou et al., 2000). VO_0000286 In three separate experiments, pCMV-Sjc97, inoculated intramuscularly three times at doses of 100 or 200 μg per C57BL/6 mouse, was found to provide significant worm reduction rates. In addition, there was a substantial reduction in the number of eggs present in the livers and spleens of the pCMV-Sjc97 immunized group when compared with the pCMV blank vector control groups. Therefore, preliminary results suggest pCMV-Sjc97 can confer protective immunity in C57BL/6 mice (Zhou et al., 2000). Chinese S. japonicum DNA vaccine pVAX/SjFABP encoding FABP VO_0004457 DNA vaccine Research pVAX1 [Ref2392:Wei et al., 2009] Intramuscular injection (i.m.) IL-18 (Wei et al., 2009) Intramuscular injection (i.m.) DNA vaccine construction Vector pVAX1 expressed Schistosoma japonicum 14 kDa fatty acid binding protein (SjFABP) (Wei et al., 2009). VO_0000286 The mice inoculated intramuscularly with pVAX/SjFABP showed significant worm reduction rate of 22.94% (P < 0.05) and egg reduction rate of 30.23% when compared with the pVAX1 control group. The addition of the pVAX/mIL-18 plasmids dramatically enhanced protection with worm reduction rate of 36.92% and egg reduction rate of 43.23% (P < 0.05), compared to the pVAX/SjFABP group (Wei et al., 2009). Chinese S. japonicum DNA vaccine pVIVO2-IL12-Sj23 encoding Sj23 and IL-12 VO_0004460 DNA vaccine Research pVIVO2 [Ref2394:Gan et al., 2004] Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction Vector pVIVO2 co-expressed S. japonicum integral membrane protein Sj23 and murine cytokine IL-12 (Gan et al., 2004). DNA vaccine construction Vector pVIVO2 co-expressed S. japonicum integral membrane protein Sj23 and murine cytokine IL-12 (Gan et al., 2004). VO_0000286 A 45.53% reduction in worm burden as well as a 58.35% egg reduction rate were found in mice vaccinated with pVIVO2-IL12-Sj23. The multivalent DNA vaccine pVIVO2-IL12-Sj23 induced stronger protection than the monovalent vaccine pVIVO2-Sj23 (P<0.05) (Gan et al., 2004). Chinese S. japonicum DNA vaccine SjC23 VO_0004512 DNA vaccine Research pVAX1 [Ref2393:Da'dara et al., 2008] Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction pVAX1 expressed SjC23 (Da'dara et al., 2008). VO_0000286 This vaccine reduced worm burdens by 45.5% and fecal miracidial hatching by 47.4% after challenge (Da'dara et al., 2008). Chinese S. japonicum DNA vaccine SjC23-Hsp70 VO_0004511 DNA vaccine Research pVAX1 [Ref2393:Da'dara et al., 2008] Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction pVAX1 expressed SjC23 and bovine heat shock protein 70 (Da'dara et al., 2008). VO_0000286 Worm burdens were reduced by 50.9% and fecal miracidial hatching by 52.0% after challenge (Da'dara et al., 2008). Chinese S. japonicum DNA vaccine SjCTPI VO_0004458 DNA vaccine Research Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction This DNA vaccine expressed triose-phosphate isomerase (SjCTPI) (Da'dara et al., 2008). VO_0000286 Worm burdens were reduced by 41.5% and fecal miracidial hatching were reduced by 33.2% (Da'dara et al., 2008). Chinese S. japonicum DNA vaccine SjCTPI-Hsp70 VO_0004459 DNA vaccine Research Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction This DNA vaccine expressed triose-phosphate isomerase (SjCTPI) fused to bovine heat shock protein 70 (Da'dara et al., 2008). VO_0000286 This vaccine produced a 51.2% reduction in worm burden, 61.5% liver egg reduction, 52.1% fecal egg reduction and 52.1% reduction in fecal miracidia (Da'dara et al., 2008). Chinese S. japonicum M6-Sj-F1 Vaccine Vector Recombinant vector vaccine Research The bacterium Streptococcus gordonii is used as the vaccine vector, with the Streptococcus pyogenes protein M6 designed to surface-localize the S. japonicum antigen on S. gordonii [Ref2886:Wang et al., 2013]. intranasal immunization Because chemotherapy is currently the main effective method to treat schistosomiasis, but it doesn't prevent reinfection, there has been a need to create a vaccine that can safely express the pathogenic antigen and elicit protection against the disease. This vectored vaccine has been shown to partially protect laboratory mice from S. japonicum challenge, making it a good candidate for schistosomiasis prevention (Wang et al., 2013). intranasal immunization The Sj-F1 antigen of S. japonicum (Wang et al., 2013). The mice inoculated with the vaccine showed strong serum IgG, serum IgA, and saliva IgA antibodies against the antigen Sj-F1 (Wang et al., 2013). VO_0000287 The mice immunized with the vaccine showed "significant protection" including a 21.45% reduction in the number of parasitic worms present and a 34.77% reduction in the number of eggs (Wang et al., 2013). The mice were challenged with the cercariae (parasitic larva) of S. japonicum (Wang et al., 2013). The protection received shows that the S. gordonii vector expressing S. japonicum is highly immunogenic and has potential to become a safe, effective vaccine against schistosomiasis (Wang et al., 2013). licensed Schistosomiasis human vaccine Generic Unknown VO_0001229 Subunit vaccine Licensed A generic representation of vaccines utilized to prevent Schistosomiasis infection in humans, typically based on purified Schistosoma antigens such as recombinant proteins. These subunit vaccines aim to induce protective immunity without using live or whole parasite components. rAdV-SjTPI.opt VO_0004782 Recombinant vector vaccine Research Intramuscular injection (i.m.) Replication-defective adenoviral vector-based vaccine with optimized SjTPI (rAdV-SjTPI.opt) (Dai et al., 2014). Intramuscular injection (i.m.) Mice were randomly divided into different vaccination groups (16 mice/group) for intramuscular (i.m.), subcutaneous (s.c.) and oral immunization (o.i.). Groups consisted of vaccination with the adenoviral vector alone or without vaccination were used as vector or normal control, respectively (Dai et al., 2014). VO_0003057 Results showed that intramuscular rAdV-SjTPI.opt induced Th1 biased immune responses in the host, while subcutaneous rAdV-SjTPI.opt induced Th2 predominant immune responses. Oral rAdV-SjTPI.opt induced low levels of immune responses and no significant protection. Intramuscular rAdV-SjTPI.opt provided a consistent and repeatable higher protective effect in mice (Dai et al., 2014). Each mouse was challenged with 40 ± 1 S. japonicum cercariae by abdominal skin penetration two weeks after the last vaccination (Dai et al., 2014). S. gordonii -M6-Sj-F1 VO_0004668 Recombinant vector vaccine Research Intramuscular injection (i.m.) Intramuscular injection (i.m.) FABP Schistosoma japonicum 349804 CDD:278490 6182 ? fatty acid binding protein 9.01 13671.32 198 Lipocalin / cytosolic fatty-acid binding protein family; pfam00061 >AAA64426.1 fatty acid binding protein [Schistosoma japonicum] MSSFLGKWKLSESHNFDAVMSKLGVSWATRQIGNTVTPTVTFTMDGDTMTMLTESTFKNLSVTFKFGEEF DEKTSDGRSVKSVVTKDSESKITHTQKDSKNTTVIVREIVGDTMKTTVTVDDVTAIRNYKRL Protective antigen Il12a Mus musculus 16159 226874945 AC121306 NP_001152896 10090 3 68690643 68698549 + transcript variant 1 8.33 25111.9 236 Also known as p35; Ll12a; Il-12a; IL-12p35 >NC_000069.6:68690643-68698549 Mus musculus strain C57BL/6J chromosome 3, GRCm38.p4 C57BL/6J CTGCCACCTACTCCCTTGGATCTGAGCTGGACCCTTGCATCTGGCGTCTACACTGCTGCTGAAATCTTCT CACCGTGCACATCCAAGGATATCTCTATGGTAAAAGTCCAGAAAGGCTAAAGATGTTGTCCACAATCTCT CGGCAATGACCAGATATATCCCCACCAGTATCACAACTTGCAACGCGCTCCGTTCCCATCTGCCCTCTGC CTCCCTACTGTGTTGATTCCCATTTCCAAAACTAGCTCAGAATGCATCCCACTGGCAGCCAGGCAATGCC TTCTTGCAGATCTGGAGGCTTCAGGAGCCTTTGCTGCCTCTTAGGCTCCTGCAGAGAGATGGCCTTGCTC TCTCTGGGCCCTTTTCCCCCAAGATGCTGTGCAGTCGTCTCACTAAATCAAAACTGCCAGAACTGAGTTT TAAGCGATATATAGCCACCACATCTATTTTGTTCCTTTATACTTTCAAAGTAGGACAGATTCGCAAAATA CATTGAGTCAGACTCAGTGTCCACGATGGAGAGACGAGTGGGTAGCTTGTGCTTCTTTAGAAGGGGCACT GGGCAGCGTGTTAGGGCTAGGGGGTAGTGGGGCAGGACAGACCTGTCCCGGGACAAGAGTGGCTACTCGC AGGCCGGTTTTGCTTTCGCTCTGAGTGTGGTGGGCGGTGGGACGGTCCCGAATCTCCTCAAACGGGCACT CCGAGAGTTAATTCGAAAGCGCCACCAGCCTTGGGGGCGATCGACGCACTTGTCCTTGAGATGTAGAGAC AGAGAGAGAAAGCAAGAGACACAGTCCTGGGAAAGTCCTGCCGGCTATCCAGACAATTATAAAAATGTGT CTCCCAAGGTCAGCGTTCCAACAGCCTCACCCTCGGCATCCAGCAGCTCCTCTCAGTGCCGGTCCAGCAT GTGTCAATCACGCTGTAAGTACCTGAGGGCCGGGACCAGGGTGACTTGATGAGAGGAGAAGGCTTGAGAA AGAACAGACAGAAGCCCTGGACCCATTAAGGGAGACTAAAGGGCTGCCAGCAACTGCAGGCCAAGGGAAG GAAGAGGGGAAAGATGGTCGTGCTAGATTGCGCTGCACCGAGCATGGTCTCATTATAGAACAACCCTGGG GGCTACGGCTATGCCCGTTTCACAGAAGAGAACTAAAGCCCAGCCTTGAAACCAATGTAAGGAAAATGCA GGTCTCAGGCAGTCCCCAAACAAGATCTGGGAGGTCTTGCTTCATCTGTCAGCCCCGGGGAAGGGCTTAA ATTATGTGTGGAGGGAGGTCAGTACCCAGAATGGATGGAAAAAATTGCCAGAATGGCTGAAGAAAGAGGC AGGTCAAAACCAAGTTGACAAGCTCCACCTGCCCTTTGTTTCCCTTCCAGACCTCCTCTTTTTGGCCACC CTTGCCCTCCTAAACCACCTCAGTTTGGCCAGGGTCATTCCAGTCTCTGGACCTGCCAGGTGTCTTAGCC AGTCCCGAAACCTGCTGAAGACCACAGATGACATGGTGAAGACGGTGAGTTTCTCCTGTCCTTCTGGCTT CAAAGTTCCGCTGTTGACATGACTTCTAACCTATCTGGTATCTGCTGGGAAGTTTATGGTATCTGGCAGG AACCGTAGAGGGTTTCAAGACTTCTAAATATAATCTAAAGGAAACTGTGTCTCTAGAGAAAGGAAGAATG GTAATAAATTGTAACGGCATCGGTTTAGGCTGTGTCTACACAGAGGAAAGTGGGGACTTGCAATGTCCCT CCCAGCCTGCCTGCCTGTGTGACACAGTGTCAGGTGCTCCCTGCTTCTGCTTCAGGGCAGGTGGAACAGG GAGACAGTCTCTAGGTACACTCTCTGTGCTTCACAAGCCATAGAGATGAATTAGATTGTCTCTCCCCCTG CTCACCAGTGTCCAGCACTGTGATGCAGTCCCTTGTCGGATGGACACATGTCAGGGAGGACTCAGACATC AGATAGCAAGCAGGTCCAGCCTCCAGTTTTCTGGGGCAAGTGTGATCCAGTTTTAGTCACGGCTCCACTC ATTTTTCTCAGGTTTCATGTTTGAATGCAAAATAGCCTTTTCCGTTGTAAAATTGCCACGGTTTCCTCTC GCTTTGGGGACAAAGCTACAGCAAAATGCTAATGTGTTTGAGATATCCTGCAGCTGCTAGGGCTGGAGAT CCAGTAGAATTCTGGCTTAATTATGAATGGGTCTCCCGGGTTCTATCCCCCAACAGAGCGTGCGTTCATA AGCACCTGGGAACAGAGGAGGGAAGGAGGGAAACTCTGTCTTCCTGTTTTGTTTTTAAATCTTTCTTGAG GATCTTCTAGCTTGTTGTCTTATGGCTATCCCAACATAAGCTCCGCTCCACAAGCCCTTCTTGGTGAATT TTCTTTCTTTCCTTCCCGTCTTGCATAACGTGCTGCTGGCTGTTCCTCCAAGCTCAGTCTTAATTGAGTG GGGTTCCCAGGAGGGAGAGATGGCTCTGTGGTTAAGAGAGCTGGCTGCTCTTCCAGAGGTCCAGAGTTTC CACACCCTCATGGTGGCTCACAATCATGGTAACTCTGATCATAGGGAACCTGTCGTTTTCTCTGTGCCTC TGTGGGCACTGAGCACATGCCACACAGACATCCATGCAGGCAGAACACTCTTGGCCATGAAACCTTAAAA TGAAGGAGAAACCTTACGAGAGGTTCCCTAAGGAATCCCTGCAGAAGGGAGAGATTGTTTTCCTAAGATG AGGTTTGGTGTGGAGGGGATGAGGTATTCAAAGTTAACTAGCCGCTCCTCACTCCTCTGCTGCCAAATAC CAGCACTGGGCTTCGAGACACAATAGAATTCGACCTTGTTTTCGTTTGGGTCACTATTGCTCTCTAGTTG AACACGGAATCCACGGGGGCACATTGCTGTCTGTCCTTTGTCACACCCCAGGACCACAGCTGCATGGAAA AGTCATCGCAGGCTTCAGTCAGAGAGCTGTTGACTTCCTAATATGTTGTGTAATTCTGTTCAGTGGTGCT TTCCAGCACACCAGGGAATAAGAGACAGTCCCCGCGTATGTAAATTAAAGCTCAACTAGTATTTAGGGCT TGCAAGTTTGTTACAGCCATCAAGCTGTGACTTTAACAGTTTCCTCTTTAGAAGTTAAACTTGAAAAAAA ATAGAGGGAGAGAGAGATTCACAGGGCACAGCTGCTTGGAAAAAGGAAAAGCCACAGGACTCACCAGAAG CAAGCAGCACAGTGTGGCACTGATGCTGATGAGTACAGCCTGCTGCACCCCAGTGTTCACATGGTGAAAA AAATACCCTGCTGGCGGACAGCCTAGGCAAAGGGGGAGGAGAGTAGGGAGAGCGTGCGGGAGGTTGGGTT TCTTAGCTTATGAAGAGCCTGAGTTTCCTACAGGCCAAAGAGATGACGGTTCAGAGTGGCCAGCCTAGGC TCTGCTGATCCACTGCGATGCAGGTCTCAGAGGGAACCAGCTGTCTTGCTGAGAGATTCACCTCAGCTTC TCCTTATGTTTTTCCCAGGCCAGAGAAAAACTGAAACATTATTCCTGCACTGCTGAAGACATCGATCATG AAGACATCACACGGGACCAAACCAGCACATTGAAGACCTGTTTACCACTGGAACTACACAAGGTATGGAG GACCCCCACCCAAAAAACAACAACAACAACAACAAAAAACACAATTAAGAGCCGTGCAGCCAACCTCAGC TGTCTCCAGATACTTACTTGTAGCTATCAAACGTGAGAGTTTTGAAGAAAAAAAAAAAAGTCAACAACCA TACAAATACTTCTACAAGCTGAAGGATATACTCGGTAACTAGGAGGTGGCTATGGTGGTATAGACCTTTA ATTCCAGCACTTGATGGATAGAGGAAGGCCGGCTCCTGTTAAGTTAGAGGCCAGTCAGGGATACACAGCA AGATTTCATCTTTAAAAAGAAAGGGGGTGGTGTGGCGGGTGAAGCTTACTTATTGAAGTGCTCATGTCAC AAGCCTGGGGACCTAAGTTCCCATCCCCAGCACCCATGTAGGAGCTGGGTGTGATGGTTTCACATGCAGA GACAGGAGGATACCTGGGCCAGCTAGTCTAGCCGAATCCATGAGCTGCAGGCCCAGTGAGAGAACCTGTA TCAGAAAACAAGAAAGTCAGGAGAGCGGCTGAGAAAGACCCCAAAGATCATCCTCCGATTTCCACGAGTA CATGCACCTGCACACACGTGCACACAGGAATCCTATACATAGCCACCCAAAAGCAGTTATGATGGTGAAG TTTCTTTTACTGGAATGAAAAGTTAGTTTGAGAAAAAAATATCAGAGAGATTTATATTAAGAGTGAATGG GTGGGGGGTATGCATGCCTTTAATCTCAGCACTTGATAGGCAGAGGCAGATGGATCTCTGTGAGTTCAAG GCCGGCCTGGTCTACAGAGTAAGTTCCAGGACAGCCAGTGCTACAATAGAAACAGGTGATGGTGATGATG GTGATGATGGCGGTGATGATGATGATGATGACGAAGACAAACTCCTCGGTTTATGATTTTCTTCTAGAAC GAGAGTTGCCTGGCTACTAGAGAGACTTCTTCCACAACAGTAAGTAAGCACTCTAAGGGTTCCTTCCCCA TGACGGATTCATAACACTGATGCCTGGTCATTCTTTCTCTAGAGAGGGAGCTGCCTGCCCCCACAGAAGA CGTCTTTGATGATGGTAAGCCACATCACTTTTCTGAAATGCAGCAGGGTCCTGTAGTTCCAGCCCATCTC AATGATTCCTTGCCCATTGTGTCCTGCGCTCTAGACCCTGTGCCTTGGTAGCATCTATGAGGACTTGAAG ATGTACCAGACAGAGTTCCAGGCCATCAACGCAGCACTTCAGAATCACAACCATCAGCAGATCATTCTAG ACAAGGGCATGCTGGTGGCCATCGATGAGCTGATGCAGGTAAGCCTTCCTCCTATCAGCCAGCGCTCCTT TTCCATGGTGAAGAGAGGTCCTTTATTAGATATGATAAAATCAGCACAGCTTGGGTTGGGAGAGACCTGA ACACCCCCACCCCACCCCACACCCTCATCCCCCAACCATGAAAGGCCTTGCAAGGTTACATTTGCTTAGA GAGCCTTGAGGGGGCCTCTACAATAAGGGCAAATGCAAGCAGCCTTCTACTTGTTGACTCATGATACAAA TCAGGCACTTTTCAAATGCTTCTTCTCATACCATCCCCACAACAAGCAAATAAGGTCTCTACAGAGGAGG AAACTGAAGCTTAAGAGGATGAACGAGGGTTCCCTACAAAAGGGTTTTTTGTTGTTTGGTTTGGTTTGGT TTGGTTTGGTTTGGTTTGGTTTTTCCGAGACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTGGAACTCAC TTTGTAGATCAGGCTGGCCTCGAACTCAGAACTCCACCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGG TGTGCGCCACCATTGCCCAGCTTATTGTTGTTTTAAATACATTCTTTTCTTAAAGGTTCAAGATTAGGAC TGGAGAGACAGTCCGATGATTAAGAGGACTTGAGTTTGGCCTGGGGCTCCTATGTAACAAACAGGGTGTG GTCCTGAACTGCTCTCTAACTCCAGAGAGTGGAGACAGGAGGCCTATGGAGGCTTTCTTGCTGCAAGTTT AGCCATGAAATCTCCAGGTTCAGGGAGAAAGCATGCCTCAAAGAAATAAGATGGAGAGTCACGTGATCTC TTCTCTGGCTTCTGCATGGTGTAGGCACAGATATGCACATGTTTGAGTGCATACAATACAGGCAAACATA CACGTGTCCTTTTTAAAATGTATAGGATGGACATTCTAGGGGCTGCACACATATTCAAATATTACTAGGG GAAGCACTTGGGGCTGGTGTCCAAGTATAATCTATTTTGAACCAAAATGTGACCAAAATGAAAAGTGGTC TTACATCTAGGTAATTACAGTGTTAGGTATCCGTGGGAACTTTGCTGTACCACCAGTAACACAAAGCACA GGAGTGTGCACACCAGAGACAAGCACCTTGGCAACCAAGGCGTGGGATGAACATATGTTCAGAAGTCACT GCCTGGAAAGCAATTGTTTTTCATTTCATACATACTGTCTTGCACAGGGTTTCTATGTCTTAGATAAAAT ATCATGACGGAAAGCAAGTTGGGGAGGAAAGGGTTTATTTCATCTTGTAGAGTGTAGTGAGTCTATTACC CAGGGAAGTGGAGGCAGAAGCTGATGCTGGAGAGGACTCCTGGTGACTGGAGTGCTCCACATGACTTGCT CAGTGTGCTTTGTATAGCACCCAGGACCACCAGGCTAGGGTGAGCTGGTGCCTCCCACATCAACTGTCAA TCAAGAAAATCACCAGAGGCTTGCCCACTGGCCAATCTGGCTGGAGGTGTTTTTCTCAATGACCACAGGC TTGTGTAGAGTTAACATAAAAGCACCCAGGACATTACATATTGCCAGATGACTCCTTTGAAAGACAGTGA AGTTTATGCAGGTCAAACAACCTATCATGTAGAGATTTGTAATGGATATTGCATGAACAAGGCCAGAAGT CAGGTGATTTGGATAGTAGGAAGAGGTCTACCAGGGACCAGCCAACTATGCTGACAGGGTTAACTCTTGT CAGCCTGTTTGTAAAATAAGATGACTTTAAATAGCCTATGTCTCTCTTGAGATTTTTGTGAGGAGCAAGC AAAAATCTTAAACATCACAGAAACCTAAGTTCTTATTCTATGTTATGAAATCAAAGGATCGAGTGGGGAA ACACGGTACTGGCTTGTTTATCTACCACAAATAGCGTTTCTATCAGAGATAGGTCTGTTGTTGATAATAA GCTCTAATCCATAGATCCTACTTACCCTCCAACTCCCTCCTCTAAAAGAACAGGACTTCTTAACATAATT TATAAAAATTAATTAGCTATTGCTGCAGGTGCTACCTCATAGACTCACACCTTCTGAGAGATCAGCTCTA ATGACAATTTCTTTCTGGAAAATTTAGGGGTCTTGTCAGAGATCATTAAAAACATAGGTATATGCTCACA AAAGAGTATTTCTTCCCTCTTTTCCTGCAGGAACACAGCAGGAGTAGATGTGAAATGGGTCTAGGAGTGT GATGAGGGGCTAGGTTGCAGGCCCTGGACTTTGCATTGACTGTCTCCCATTTTGCAGACAAACATTCTAA TTTTTAAATTGTGCATATGCTATGCTTGGTGTTTCTTTCTAGTCTCTGAATCATAATGGCGAGACTCTGC GCCAGAAACCTCCTGTGGGAGAAGCAGACCCTTACAGAGTGAAAATGAAGCTCTGCATCCTGCTTCACGC CTTCAGCACCCGCGTCGTGACCATCAACAGGGTGATGGGCTATCTGAGCTCCGCCTGAAAGGCTCAAGGC CCTCTGCCACAGCGCCCTCCTCACACAGATAGGAAACAAAGAAAGATTCATAAGAGTCAGGTGGTCTTGG CCTGGTGGGCCTTAAGCTCCTTCAGGAATCTGTTCTCCCATCACATCTCATCTCCCCAAAGGTGGCACAG CTACCTCAGCATGGTCCCCTCCATCGCTTCTCTCATATTCACTATACAAGTTGTTTGTAAGTTTTCATCA AAATATTGTTAAGGGGCGAAGACGTCCTCCCCTCAATGTGTTAGCAGAAGAGCAAGAACTGATAAGCTAT TGTTTTTGTGCCAAAGTGTTTATGAAAACACTCAGTCACCCCTTATTTAAAAATATTTATTGCTATATTT TATACTCATGAAAGTACATGAGCCTATTTATATTTATTTATTTTCTATTTATTATAATATTTCTTATCAG ATGAATTTGAAACATTTTGAAACATACCTTATTTTGTGGTTCTAATAAAGTAATGTTATCACTTCAC >NP_001152896.1 interleukin-12 subunit alpha isoform 1 [Mus musculus] MVSVPTASPSASSSSSQCRSSMCQSRYLLFLATLALLNHLSLARVIPVSGPARCLSQSRNLLKTTDDMVK TAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTTRGSCLPPQKTSLMMTLC LGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKM KLCILLHAFSTRVVTINRVMGYLSSA Protective antigen Paramyosin Schistosoma japonicum 4761226 CDD:278467 CDD:283846 6182 ? paramyosin 5.01 97539.21 927 Intermediate filament protein; pfam00038 >AAD29285.1 paramyosin [Schistosoma japonicum] MMNHDTESHVKISRTIYRGVSPSTTRLESRVRELEDILDLERDARVRAERHAADLSYQVDALSERLDEAG GSTTQTQELLKRREMEINKLRKDLENANASLELAETSMRRRHQTALNELSLEVENLQKQKGKAEKDKSHL IMEVDNVLGQLDGALKAKQSAESKLEGLDSQLNRLKTLTDDLQRQLTELNNAKSRLTSENFELLHINQDY EAQILNYSKAKSSLESQVDDLKRSLDDESRNRFNLQAQLTSLQMDYDNLQAKYDEESEEASNLRNQVSKF NADIAALKSKFERELMSKTEEFEEMKRKLTMRITELEDVAERERLKAVSLEKLKTKLTLEIKDLQSEIES LSLENGELIRRAKSAESLASELQRRVDELTIEVNTLTSQNNQLESENMRLKSLVNDLTDKNNALERENRQ MNDQVKELKSSLRDANRRLTDLEALRSQLEAERDNLASALHDAEEALRDMDQKYQASQAALNHLKSEMEQ RLRERDEELESLRKSTTRTIEELTVTITEMEVKYKSELSRLKKRYESSIADLEIQLDATNKANANLMKEN KNLAQRIKDLETFLDDERRLREAAENNLQITEHKRIQLANEVEELRSAMENLERLRKHAETELEETQSRV SELTIQVNTLSNDKRRLEGDIGVMQADMDDAINAKQAAEDRATRLNNEVLRLADELRQEQENYKHAEALR KQLEIEIREITVKLEEAEAYATREGRRMVQKLQARVRELEAEFDGESRRCKDALAQARKFERQYKELQTQ AEDDRRMVLELQDLLDKTQMKMKAYKRQLEEMEEVSQITMNKYRKAQQQIEEAEHRADMAERTVTVRRVG PGGRAVSVARELSVTSNRGMRATSMM Protective antigen Sj23 Schistosoma japonicum 124390 CDD:278750 CDD:239401 6182 ? 23 kDa integral membrane protein 6.08 22840.74 314 Sj23 >sp|P27591.1|IM23_SCHJA RecName: Full=23 kDa integral membrane protein; AltName: Full=Sj23 MATLGTGMRCLKSCVFILNIICLLCSLVLIGAGAYVEVKFSQYEANLHKVWQAAPIAIIVVGVVILIVSF LGCCGAIKENVCMLYMYAFFLIVLLIAELVAAIVAVVYKDKIDDEINTLMTGALENPNEEITATMDKIQT SFHCCGVKGPDDYKGNVPASCKEGQEVYVQGCLSVFSAFLKRNLIIVACVAFGVCFFQLLSIVIACCLGQ RIHDYQNV Protective antigen TPI Schistosoma japonicum 2501312 CDD:238190 6182 ? Triosephosphate isomerase 7.47 27589.91 374 TIM; Triose-phosphate isomerase >sp|Q27775.1|TPIS_SCHJA RecName: Full=Triosephosphate isomerase; Short=TIM; AltName: Full=Triose-phosphate isomerase MSGSRKFFVGGNWKMNGSRADNKVLIHSLSEAHFCGDTEILIAAPFVYLNEVRQSLAKEIHVAAQNCYKV PKGAFTGEISPSMIKDVGCDWVILGHSERRSIFNESDELVAEKVQHALAEGLSVVACIGETLSERESGKT EEVCVRQLNAIANKIKSIDEWKRVVVAYEPVWAIGTGKVASPGQAQEVHNFLRKWFKTNTPSGVDQQIRI IYGGSVTAANCKELAQQHDVDGFLVGGASLKPEFIDICKARQ Protective antigen Balen et al., 2007 journal Balen J, Zhao ZY, Williams GM, McManus DP, Raso G, Utzinger J, Zhou J, Li YS 2007 85 7 519-526 Bulletin of the World Health Organization Chen et al., 2016 journal Chen L, Chen Y, Zhang D, Hou M, Yang B, Zhang F, Zhang W, Luo X, Ji M, Wu G 2016 38 10 589-598 Parasite immunology Da'dara et al., 2008 journal Da'dara AA, Li YS, Xiong T, Zhou J, Williams GM, McManus DP, Feng Z, Yu XL, Gray DJ, Harn DA 2008 26 29-30 3617-3625 Vaccine Gan et al., 2004 journal Gan Y, Shi YE, Bu LY, Zhu XH, Ning CX, Zhu HG 2004 117 12 1842-1846 Chinese medical journal Gao et al., 2017 journal Gao Y, Zhou X, Wang H, Liu R, Ye Q, Zhao Q, Ming Z, Dong H 2017 116 6 1665-1674 Parasitology research Ke et al., 2017 journal Ke XD, Shen S, Song LJ, Yu CX, Kikuchi M, Hirayama K, Gao H, Wang J, Yin X, Yao Y, Liu Q, Zhou W 2017 10 1 89 Parasites & vectors Wei et al., 2009 journal Wei F, Liu Q, Zhai Y, Fu Z, Liu W, Shang L, Men J, Gao S, Lian H, Jin H, Chen C, Lin J, Shi Y, Xia Z, Zhu XQ 2009 111 3 284-288 Acta tropica Zhou et al., 2000 journal Zhou S, Liu S, Song G, Xu Y, Sun W 2000 18 27 3196-3204 Vaccine