Experimental autoimmune uveitis Experimental autoimmune uveitis Experimental autoimmune uveitis (EAU) is an organ-specific, T cell–mediated disease that targets the posterior pole of the eye. It is also a valuable, well-characterized model for the study of human idiopathic uveitis. This disease can be induced in susceptible primates and rodents after immunization with retinal self-antigens, such as interphotoreceptor retinoid-binding protein (IRBP) or S-antigen (arrestin), or by the adoptive transfer of uveitis antigen-specific T cells (Commodaro et al., 2010). Baboon Papio cynocephalus 9556 Bank vole Clethrionomys glareolus 447135 Bear Ursus americanus 9643 Birds Passeroidea 175121 Brown Trout Salmo trutta 8032 Buffalo Bison bison 9901 Carnivores Vulpes 9625 Cat Felis catus 9685 Catfishes Siluriformes 7995 Cattle Bos taurus 9913 Chicken Gallus gallus 9031 Chimpanzee Pan troglodytes 9598 chinchillas Chinchillidae 10150 Copper Pheasant Syrmaticus soemmerringii 9067 Deer Cervus elaphus 9860 Deer mouse Peromyscus maniculatus 10042 Dog Canis familiaris 9615 Ducks Anas 8835 Ferret Mustela putorius furo 9669 Fish Hyperotreti 117565 Gerbil Gerbillina 10045 Goat Capra hircus 9925 Gray wolf Canis lupus 9612 Guinea pig Cavia porcellus 10141 Hamster Mesocricetus auratus 10036 Horse Equus caballus 9796 Human Homo sapiens 9606 Macaque Macaca fascicularis 9541 Mongolian Gerbil Meriones unguiculatus 10047 Monkey Platyrrhini 9479 Mouse Mus musculus 10090 None None Parrot Psittacidae 9224 Pig Sus scrofa 9823 Rabbit Oryctolagus cuniculus 9986 Rainbow trout Oncorhynchus mykiss 8022 Rat Rattus 10114 Raven Corvus corax 56781 sei whale Balaenoptera borealis 9768 Sheep Ovis aries 9940 Squirrel Spermophilus richardsonii 37591 Tree shrew Tupaiidae 9393 Trouts, salmons & chars Salmoninae 504568 Turkey Meleagris gallopavo 9103 Vole Microtus ochrogaster 79684 Water buffalo Bubalus bubalis 391902 EAU DNA vaccine IRBP-DNA encoding IRBP VO_0004402 DNA vaccine Research pcDNA3.1 [Ref2314:Silver et al., 2007] Intravenous injection (i.v.) Intravenous injection (i.v.) DNA vaccine construction Vector pcDNA3.1 expressed the uveitogenic retinal Ag interphotoreceptor retinoid-binding protein (IRBP) (Silver et al., 2007). IRBP-specific CD4+CD25high T cells could be cultured from vaccinated mice and transferred protection to unvaccinated, EAU-challenged recipients. Expression of IRBP in the periphery by DNA vaccination results in tolerance that acts at least in part through induction of IRBP-specific, FoxP3+CD4+CD25+ regulatory T cells (Silver et al., 2007). VO_0000286 Vaccinated mice were highly protected from EAU induced by immunization with IRBP for at least 10 weeks after vaccination (Silver et al., 2007). IRBP from humans Homo sapiens 5949 4506453 AH002852 NP_002891 9606 10 47348370 47357874 + retinol binding protein 3 4.76 124350.97 1247 Also known as IRBP; RBPI; D10S64; D10S65; D10S66 >NC_000010.11:47348370-47357874 Homo sapiens chromosome 10, GRCh38.p12 Primary Assembly CTGTCCACCAGCTGAGAAGGACAAGGGCGGAAGGCAGCTGCACAGAGCAGGGCCACGGCCTTGCACACAG TCCAGGGAGCTTTTGTGCAGGAGCCAGGCCTCCCCCTGGGTCCCCATGATGAGAGAATGGGTTCTGCTCA TGTCCGTGCTGCTCTGTGGCCTGGCTGGCCCCACACACCTGTTCCAGCCAAGCCTGGTGCTGGACATGGC CAAGGTCCTCTTGGATAACTACTGCTTCCCGGAGAACCTGCTGGGCATGCAGGAAGCCATCCAGCAGGCC ATCAAGAGCCATGAGATTCTGAGCATCTCAGACCCGCAGACGCTGGCCAGTGTGCTGACAGCCGGGGTGC AGAGCTCCCTGAACGATCCTCGCCTGGTCATCTCCTATGAGCCCAGCACCCCCGAGCCTCCCCCACAAGT CCCAGCACTCACCAGCCTCTCAGAAGAGGAACTGCTTGCCTGGCTGCAAAGGGGCCTCCGCCATGAGGTT CTGGAGGGTAATGTGGGCTACCTGCGGGTGGACAGCGTCCCGGGCCAGGAGGTGCTGAGCATGATGGGGG AGTTCCTGGTGGCCCACGTGTGGGGGAATCTCATGGGCACCTCCGCCTTAGTGCTGGATCTCCGGCACTG CACAGGAGGCCAGGTCTCTGGCATTCCCTACATCATCTCCTACCTGCACCCAGGGAACACCATCCTGCAC GTGGACACTATCTACAACCGCCCCTCCAACACCACCACGGAGATCTGGACCTTGCCCCAGGTCCTGGGAG AAAGGTACGGTGCCGACAAGGATGTGGTGGTCCTCACCAGCAGCCAGACCAGGGGCGTGGCCGAGGACAT CGCGCACATCCTTAAGCAGATGCGCAGGGCCATCGTGGTGGGCGAGCGGACTGGGGGAGGGGCCCTGGAC CTCCGGAAGCTGAGGATAGGCGAGTCTGACTTCTTCTTCACGGTGCCCGTGTCCAGGTCCCTGGGGCCCC TTGGTGGAGGCAGCCAGACGTGGGAGGGCAGCGGGGTGCTGCCCTGTGTGGGGACTCCGGCCGAGCAGGC CCTGGAGAAAGCCCTGGCCATCCTCACTCTGCGCAGCGCCCTTCCAGGGGTAGTCCACTGCCTCCAGGAG GTCCTGAAGGACTACTACACGCTGGTGGACCGTGTGCCCACCCTGCTGCAGCACTTGGCCAGCATGGACT TCTCCACGGTGGTCTCCGAGGAAGATCTGGTCACCAAGCTCAATGCCGGCCTGCAGGCTGCGTCTGAGGA TCCCAGGCTCCTGGTGCGAGCCATCGGGCCCACAGAAACTCCTTCTTGGCCCGCGCCCGACGCTGCAGCC GAAGACTCACCAGGGGTGGCCCCAGAGTTGCCTGAGGACGAGGCTATCCGGCAAGCACTGGTGGACTCTG TGTTCCAGGTGTCGGTGCTGCCAGGCAATGTGGGCTACCTGCGCTTCGATAGTTTTGCTGACGCCTCCGT CCTGGGTGTGTTGGCCCCATATGTCCTGCGCCAGGTGTGGGAGCCGCTACAGGACACGGAGCACCTCATC ATGGACCTGCGCCACAACCCTGGAGGGCCATCCTCTGCTGTGCCCCTGCTCCTGTCCTACTTCCAGGGCC CTGAGGCCGGCCCCGTGCACCTCTTCACCACCTATGATCGCCGCACCAACATCACGCAGGAGCACTTCAG CCACATGGAGCTCCCGGGCCCACGCTACAGCACCCAACGTGGGGTGTATCTGCTCACCAGCCACCGCACC GCCACGGCCGCGGAGGAGTTCGCCTTCCTTATGCAGTCGCTGGGCTGGGCCACACTGGTAGGTGAGATCA CCGCGGGCAACCTGCTGCACACCCGCACGGTGCCGCTGCTGGACACACCCGAAGGCAGCCTCGCGCTCAC CGTGCCGGTCCTCACCTTCATCGACAATCACGGCGAGGCCTGGCTGGGTGGTGGAGTGGTGCCCGATGCC ATCGTGCTGGCCGAGGAGGCCCTGGACAAAGCCCAGGAAGTGCTGGAGTTCCACCAAAGCCTGGGGGCCT TGGTGGAGGGCACAGGGCACCTGCTGGAGGCCCACTATGCTCGGCCAGAGGTCGTGGGGCAGACCAGTGC CCTCCTGCGGGCCAAGCTGGCCCAGGGCGCCTACCGCACAGCTGTGGACTTGGAGTCTCTGGCCTCTCAG CTCACAGCAGACCTCCAGGAGGTGTCTGGGGACCACCGCTTGCTAGTGTTCCACAGCCCTGGCGAGCTGG TGGTAGAGGAAGCACCCCCACCACCCCCTGCTGTCCCCTCTCCAGAGGAGCTCACCTACCTTATTGAGGC CCTGTTCAAGACAGAGGTGCTGCCCGGCCAGCTGGGCTACCTGCGTTTTGACGCCATGGCTGAACTGGAG ACAGTGAAGGCCGTGGGGCCACAGCTGGTGCGGCTGGTATGGCAACAGCTGGTGGACACGGCTGCGCTGG TGATCGACCTGCGCTACAACCCTGGCAGCTACTCCACGGCCATCCCGCTGCTCTGCTCCTACTTCTTTGA GGCAGAGCCCCGCCAGCACCTGTATTCTGTCTTTGACAGGGCCACCTCAAAAGTCACGGAGGTGTGGACC TTGCCCCAGGTCGCCGGCCAGCGCTACGGCTCACACAAGGACCTCTACATCCTGATGAGCCACACCAGTG GCTCTGCGGCCGAGGCCTTTGCACACACCATGCAGGACCTGCAGCGGGCCACGGTCATTGGGGAGCCCAC GGCCGGAGGCGCACTCTCTGTGGGCATCTACCAGGTGGGCAGCAGCCCCTTATATGCATCCATGCCCACC CAGATGGCCATGAGTGCCACCACAGGCAAGGCCTGGGACCTGGCTGGTGTGGAGCCCGACATCACTGTGC CCATGAGCGAAGCCCTTTCCATAGCCCAGGACATAGTGGCTCTGCGTGCCAAGGTGCCCACGGTGCTGCA GACGGCCGGGAAGCTGGTGGCTGATAACTATGCCTCTGCCGAGCTGGGGGCCAAGATGGCCACCAAACTG AGCGGTCTGCAGAGCCGCTACTCCAGGGTGACCTCAGAAGTGGCCCTAGCCGAGATCCTGGGGGCTGACC TGCAGATGCTCTCCGGAGACCCACACCTGAAGGCAGCCCATATCCCTGAGAATGCCAAGGACCGCATTCC TGGAATTGTGCCCATGCAGGTGAGACCCAAGAGAGACCTGGCTGAACCCAGTCCCGGGAGTGAGTTGACC CATTGTCCGCACATGCAGGGCTCTGTGCACAGTGCGTGACAATGGCTTTTAGATTTGTTCTCACGTTTAA GTTTTGACCGGTCAAGTCCTTTCCTCTTTCTCAACCTGTTCCATCCACTCTCTGTGACCCTGGGGTTGCT GAACACCTCTGTAGAACATTCATATTAGGTTGGTGCAAAAGTACTTTCAATGGCAAAACCCGCAATTACT TTTGCACCCACCTCACAGGAAGCCAGTTTGAAAGCCAACCAATACTCACAGGAAGCCAGTTCGGAAGCTC CTGGGATAGAAGGCATTTCAGCCTTGGCTGGGTGGAAGGTGAGTGTTGGCAGGGCTTCTCATTGTCAGTG CTAGGGAAGAGGCCAACACCTGTCAGAGGTGGCCAATGGACTTCACCAAGTGCCCCACGCTGGCCGAGAG CTCCACCTAGGCAGCACTCACACCTCCACACTGTTCTACCTGTGGTCTGCTGCATCGTCACAATTGGGCA GGGCAGCATTTGCCATGGGATCCCTTGCAAGGAGGGTCTGAGACCAGGGCTTGGGTGCAGGCGCTTTGTC TGGGAGGCGGTTACTGAAGCAGGCATGAAGGAGGGAGCAGGGAGAGTGGGTTGGGAAGCTGACAGGCAGG TGCCTCAAAGCTGTTCTGCTGAAGCCAGGACCCTGACGAGTGCAGGGATGCTCCCAGGCATAGCCCTCTG CAGGCAGGGCCCAGTGGTTCCTGTCCCGCATTGGCCAAGAGTTGCCCTGAGACATGCCTCAGGGTGGGGC AGGCTCCCTCTTCTTGGAGAAGGCCTGAGCTACGGGTGGAAAGGCAGGGCTGTGCTATAGGAGAGCCTGT CAGTGGGGTCGGGTGCAGCTGAAAGCAGAGGGGGCTGAGAGTGCCAAAAGCATCTACTACAGAATTGTTA TCCCCATTTTCCGCAACTGAGGCCCGGAGAGGAGCAGAGGGGAGTGGCCTGAGGCCAGAGAGCTGTGACT GAGGGCAGGGCAGGGCCTGGAGGGCAGTGTCTCTGTCAATGAAGTCTCCTTGCCTGTCAATCTCACCAAG ACCTGCCTCCCTCCAGCAGCCTTAGAGAGGGAGGAGGAGGTGCATCCACGTGCGAGTAGCCTGTGCTAGG CTTGCAGAATCCCCAGTTTCCAAATCAACATCTCCTTCCTTTCCAGTATAGCCAAGGTTCACGATTTGGA GTCAGATGTGGATTCAGATTCTGGCTCCACCACTTACTGACTGTGTGACCTGAGACTAGTTACTTAATCT CTCTGTGCTTCAGTTTTTCCATGGAAAAGATGGGGATCGTGTTATCTCCTGTACAGGTGGCTGTGAGGAT GATGATAAGCTCTACAAAGTGCTTAGTACCGGGCCAGGTGCCTGGTAAAGGTAACTAACATCTTCCAATC CTGCCCCAGTGGAGCAGCTTAGAGACATAGGAAGTATCTGGTAAGGTTGGAGAGGTCAGAGGGGAGTCCA TTCCTGGGCCTTCTAGCTCAGGACACCAGGGCATGTGGGTGGCAGACAGGAGCATCCTCTGCAAGGAGGC TGCCCATAGATCACACATGTCCCAGTGGCATGTCACATCCAGACATGCCACTGGGAAAGTCCCTGGTGTC TACTAATTCCTTCAGAAATGTTAGTTCCTGTCCCATGCCCTTAATATTTCCCATGGCGCCTGCTTTCCTG GGCTCTAAAACTGGCTGCTCCTCCTGACACTGAGTAGGACCTCCAACTCTTACAGATCCCTTCCCCTGAA GTATTTGAAGAGCTGATCAAGTTTTCCTTCCACACTAACGTGCTTGAGGACAACATTGGCTACTTGAGGT TTGACATGTTTGGGGACGGTGAGCTGCTCACCCAGGTCTCCAGGCTGCTGGTGGAGCACATCTGGAAGAA GATCATGCACACGGATGCCATGATCATCGACATGAGGTCAGTGGCCAGGGGTCAGTGCTTCCTAGCCAGG ACGCAGGGCTGCCAGGGGACAGTCAAAGCTATGGGCCACAGCAGGGAAGAAAAGGAACCCTGTGACACAG CAGAGGACCTGAGGGGGGCCAGGCCTCCTGCCTATCAGGGCTTCGGCCAGCTAGCCCAGCAGATCTGCTG TCATTGCAGGTGGGGCCCAGCACTGGTAGTTTTTGCAAGCTTCTCCAGTGATTCCAATGTGCATTCCAGA TTGAGAATTACCCCAGATTTGCCTGGTCATAGGATTCAGTGGGGAGGAGAGGAAGGAGATGTTAAAAACA CTGATTCCTGGGCCCCACTCCCCTCTTACTGACTCAGAAGCTCCAGGGGACAGGCCCGATAATCTCTATT TGACAGGCATCCCAGGTGATTCTGATGGTCAGTGGTGTGTGAGACAGCCAGGCCCCCCACCTGGGAGTGT GCCTCACATCATACAAAATCAACACATGACAACCCTGGTCATATGAATCGTACAGGCAGAATTTGGAAAT CACACAGGGAAAATTTAGAAGTTAATCACTATTGTTAACAAAAGAATTCTTTGCATCACCAAATGATTGT CAGAAGGATGTCTTCAGCTAGCCACCTCCCCTCTGTTTCTGTAAAAATGTGCCTCATATGTCATGAGTTT ACCTCATGGACCAGGGGTAGGAGGTGGATGAGTACCCTCAGGCAGTGGCCAGACCTGGCATCCCATGACT CTGGGGTCCATTGGTGGACATGCCCCAGCACCCTCCCTGGGCAGGTTGCCTTCCTCAGACGGGACCCTTC CATGCTCCCGTGTTGTCTGAAAGAAGTGGCAGGGGGTGGCAGACCAGAAGCATTCACCCAGCTCCAGTTC TCTAGCCCTCAGCGCTCACTCACATGGTTCTTAGTGGTCACTAGGTGGCTCTCGGTCCTGCACCCACTGC TTCTCCTCCCTGGGAGATAAAACATTAGCCCTGGCTCCAGTTCTGTGTGTGTTCACAGCCTGAGCTTGAG GCTCCTGGGGTCTCTCTGATCATTTTATTCTTTAGAAGTTCTGTTGAGCACCCAAATGACACTCATGCTA AGTCTCTTTCTTCTGAATATGCCATTTACACTTTTTAAAGAGGAGATGGCCCCAGCCTGCAAGTGCACTT TGCTTAAAGACCCTCCCACCCCTGAGGTTTGTTTGGAGGTTCAGACACTGACATCACAGGCCCCGAACCC AGCCAGGCCATCCCAGGCCATCAGTTGGACGAGGCTCCTTAACACATCACTAGCCCCAGTGGGGAGACAG GGGCCCAACGAGGTCACACAGTGGGACAAATCTAAATGGCCTGGGAGAAATACAGGCTCCCCTGCTCCCA ATGCAGCTCCACAAGCCTGAATATTTCAATACACTAAGTGCCCTGTTAAGACACCTGAACTAAGACAGCT GGTAGGTGTCATAAGATTGTCACAGAATGCAACGGGAAAAACAATGTTTCCCAAAATGTGTTCCACTCAT AGGAAGATATGGGTGCAGGGGAGCAGTCCATGGTCAATAAATGGGGGAAATTTATCAACAAGTTTCTGAC TGCAGGACTTGTCAGGGCCTTTAATATATTAACATGTATTAATTACATAGCAGAAGGAAAGGATAACATA TTTGATCACTAAGTTTCTTGTTCTAAAGAGACTAGTACTCTTGGAAATACTCTGGAAAATGCCAACCCAT GGAATTCTAAAGGAGGAAGAGGCACTAGACTGAGGCTGCCAAGAATTAGAACCCTCCATGGGACAAAGAT CCTGGCCTCCCCGAGGGGCACACAGGGCCTCACTGTGAGCTCAGCCCCTGAACAGGCTCTGCTTCCCATC CTTCAGGTTCAACATCGGTGGCCCCACATCCTCCATTCCCATCTTGTGCTCCTACTTCTTTGATGAAGGC CCTCCAGTTCTGCTGGACAAGATCTACAGCCGGCCTGATGACTCTGTCAGTGAACTCTGGACACACGCCC AGGTTGTAGGTACGTGGAGAAGCTTTCTCCTTTCTGCTGTCATTCTAGAAGCTTCTGGGAAACCAGGGAA AGACAGCTTGGGTGTAGGTAAAAGTCATAGTAACCAGAATGTAAGGCCCTGTCCTAGTCCAGGCACTAGA TGTGCATGATCTCGAATCCCTGAAAAGCATGTATCACTATGCCCGTTTTCTAGAGGAAAAAAAAGGGGGT GTTGACTAGGTCAAATATCTTTTCTAGGGTCATGCAGCTAATAAGTGAAACCCAAAATGTGAATATTAGT TTGTCTAATACCAAAATTTCCCCCAGGCTTAGCACCATAATCACCAGCCTGCAGGACAAATGCAGAATTT GAAGGATTCTCCCCGCCCCTCACCAACCCCAGTGGGCATTGCCAGTGCTCACAGTACTTGAGCTCTCAAG CCACAGTGCCACAAACTTGCCTGGAAAGGTCATGATGCTGATCGGGCTTCTGTTCCCCGCCCTGGGCCCA GAGCAGTGGCTATAGGAGCAAAGGCAGGCCCTGAGCTTTACAGAACCATAACTCTCAAAGCCCCATTCCA GGGTGGATGGCCTCCATCTATCTTCCCTGCCCAGTGTGTAAGACCAAGGAACTTGTGCTCCTACCCAACA AGAAAGGACTGGAGAGACCGATGGGGGTGTGCCTGTGACCAGAGAAACACCCCACCATTTTCATGTCCAT GCTTCTGTAATACACAGGCATTTTGCTGTAGCTCCTAGGACATACGTTCAATAATATTACAAGTGTAAAT GCAAATCAGAACCAGGTGAATTCTAAATTCAAATAGATGTTCAAGACTGGAAGTTAAAGATAGACTCAAT TCTGCAGATAATGAGGCCCCAAAACCGCAAGGTCCAATATGGGAGCCACAAGCCACCTGCGGCCACCAAG CATTTGAAATGTGGCTCTCCCAAGTTGAGATGTGCTGGAAGTGTGATGTGCTGGAAGTGTAATGTGCATA CCAGATTTTGAAGACATTATGAAAAGAAAACCTCATTAATAATTTTATTTGATTACATTTTGAAAAGAAT GTATTTTGGATACATTGGCTTAAACAAAACATGATTAAAATTAACACCATGGGTTTCTTTTTGCTTGCTT AACATGGCTACTAGGGAACCTAAAATGACATCTATGGCCAGCATTCTGGCTCACGTTGCATTCCTGTTGG ACAGTGCTGTCCTGGAGAGTTGTTCTTATACATCACAAATTGGGCTTTTCTGAGTCCAAAGTGAAAAGGA AGTCAGGTCGGTTTAGGTGTTCTCATTATTCATGAAGAGAGAGGATGCTCCTTTCTCAGGAGAGTCAAGC CTTTCCTATCACTTGGCATAAAAAGGCCTCGTTGGGAGTTGTCAAGCACTGAAATCCTCGTGATTGGGCA TGCATTCTTGTCCCCATTTTACGGATGCAGAAATCAAGGCTCAGAGAAGTCAAGTGGCTCACAAGTGGAC ACAAGGACACAGACCTAAACCCCCGGGCCTCCTCCATCACTGCAGGCCCAGGCAGGATAGAGAAGACAGG TGCTCCAGGGTCCTGACATGACCCCCATCCTGAAGGGCCTTATGTCTTCCAGGTGAACGCTATGGCTCCA AGAAGAGCATGGTCATTCTGACCAGCAGTGTGACGGCCGGCACCGCGGAGGAGTTCACCTATATCATGAA GAGGCTGGGCCGGGCCCTGGTCATTGGGGAGGTGACCAGTGGGGGCTGCCAGCCACCACAGACCTACCAC GTGGATGACACCAACCTCTACCTCACTATCCCCACGGCCCGTTCTGTGGGGGCCTCGGATGGCAGCTCCT GGGAAGGGGTGGGGGTGACACCCCATGTGGTTGTCCCTGCAGAAGAGGCTCTCGCCAGGGCCAAGGAGAT GCTCCAGCACAACCAGCTGAGGGTGAAGCGGAGCCCAGGCCTGCAGGACCACCTGTAGGGAAGGGCCCCA TAGGCAGAGCCCCAGGGCAGACAGAACCTCTGGGACACACACCAAGGGCACTCCTGCAGGTGGCCCGGCC TGAGGTTCCCAGGAGCAGCAAAGGGGCCTGCTGAGCTCTGGTTAGGTTACAGCTGGAGGTGTGTATATAT ACACACACACACATGTATATACACATATATATGTGTATGTATATATATGTATATATATATGGCTTTCCAA TAACCACCTAAATTTTAACAAAGGTTCCTTCTAAGTGGTAGAACTTGGGGTGGTATTTTTACCTTCCTTC TTCATACTTTGCTCTTTTTCTTAAATACTCATTAATGTGCATATATCATTATTTTCAGATGCAGCTATCA TTATTCCAAAATACAAAATAAAGAAGATAAAATAAATTATATACCCGAGCCATTA >NP_002891.1 retinol-binding protein 3 precursor [Homo sapiens] MMREWVLLMSVLLCGLAGPTHLFQPSLVLDMAKVLLDNYCFPENLLGMQEAIQQAIKSHEILSISDPQTL ASVLTAGVQSSLNDPRLVISYEPSTPEPPPQVPALTSLSEEELLAWLQRGLRHEVLEGNVGYLRVDSVPG QEVLSMMGEFLVAHVWGNLMGTSALVLDLRHCTGGQVSGIPYIISYLHPGNTILHVDTIYNRPSNTTTEI WTLPQVLGERYGADKDVVVLTSSQTRGVAEDIAHILKQMRRAIVVGERTGGGALDLRKLRIGESDFFFTV PVSRSLGPLGGGSQTWEGSGVLPCVGTPAEQALEKALAILTLRSALPGVVHCLQEVLKDYYTLVDRVPTL LQHLASMDFSTVVSEEDLVTKLNAGLQAASEDPRLLVRAIGPTETPSWPAPDAAAEDSPGVAPELPEDEA IRQALVDSVFQVSVLPGNVGYLRFDSFADASVLGVLAPYVLRQVWEPLQDTEHLIMDLRHNPGGPSSAVP LLLSYFQGPEAGPVHLFTTYDRRTNITQEHFSHMELPGPRYSTQRGVYLLTSHRTATAAEEFAFLMQSLG WATLVGEITAGNLLHTRTVPLLDTPEGSLALTVPVLTFIDNHGEAWLGGGVVPDAIVLAEEALDKAQEVL EFHQSLGALVEGTGHLLEAHYARPEVVGQTSALLRAKLAQGAYRTAVDLESLASQLTADLQEVSGDHRLL VFHSPGELVVEEAPPPPPAVPSPEELTYLIEALFKTEVLPGQLGYLRFDAMAELETVKAVGPQLVRLVWQ QLVDTAALVIDLRYNPGSYSTAIPLLCSYFFEAEPRQHLYSVFDRATSKVTEVWTLPQVAGQRYGSHKDL YILMSHTSGSAAEAFAHTMQDLQRATVIGEPTAGGALSVGIYQVGSSPLYASMPTQMAMSATTGKAWDLA GVEPDITVPMSEALSIAQDIVALRAKVPTVLQTAGKLVADNYASAELGAKMATKLSGLQSRYSRVTSEVA LAEILGADLQMLSGDPHLKAAHIPENAKDRIPGIVPMQIPSPEVFEELIKFSFHTNVLEDNIGYLRFDMF GDGELLTQVSRLLVEHIWKKIMHTDAMIIDMRFNIGGPTSSIPILCSYFFDEGPPVLLDKIYSRPDDSVS ELWTHAQVVGERYGSKKSMVILTSSVTAGTAEEFTYIMKRLGRALVIGEVTSGGCQPPQTYHVDDTNLYL TIPTARSVGASDGSSWEGVGVTPHVVVPAEEALARAKEMLQHNQLRVKRSPGLQDHL Protective antigen Commodaro et al., 2010 journal Commodaro AG, Peron JP, Lopes CT, Arslanian C, Belfort R Jr, Rizzo LV, Bueno V 2010 51 5 2568-2574 Investigative ophthalmology & visual science Silver et al., 2007 journal Silver PB, Agarwal RK, Su SB, Suffia I, Grajewski RS, Luger D, Chan CC, Mahdi RM, Nickerson JM, Caspi RR 2007 179 8 5146-5158 Journal of immunology (Baltimore, Md. : 1950)