Pasteurella multocida 747 In birds, P. multocida is believed to enter through the respiratory tract tissues of the host. P. multocida has been shown to adhere to air sac macrophages. The mode of transfer from the lungs to the bloodstream is poorly understood, but heterophils may play a role in the process (Harper et al., 2006). Pasteurellosis, fowl cholera The first systemic host defence used against most invading bacteria involves the innate immune system and includes phagocytosis and bactericidal properties of serum components such as complement. However, P. multocida has mechanisms to evade this system, including the presence of a capsule. Active immunity against P. multocida infection is mainly humoral; vaccination of birds with killed P. multocida stimulates protection against homologous challenge, and opsonization studies in mice have shown that bactericidal antibodies are produced (Harper et al., 2006). P. multocida causes can cause disease in fowl, pigs, cattle, rabbits and humans (Harper et al., 2006). Pasteurella multocida is a Gram-negative, non-motile coccobacillus that is penicillin-sensitive and belongs to the Pasteurellaceae family. It can cause a zoonotic infection in humans, which typically is a result of bites or scratches from domestic pets. Many mammals and fowl harbor it as part of their normal respiratory microbiota, displaying asymptomatic colonization. P. multocida is the most common cause of infection from animal injuries (pneumonia in cattle and pigs, atrophic rhinitis in pigs and goats, and wound infections after dog/cat-bites.) A high leukocyte and neutrophil count is typically observed, leading to an inflammatory reaction at the infection site (generally a diffuse localized cellulitis). It can also infect other locales, such as the respiratory tract. In more serious cases, a bacteremia can result, causing an osteomyelitis or endocarditis. The bacteria may also cross the blood-brain barrier and cause meningitis (Wiki: P. multocida). Baboon Papio cynocephalus 9556 Bank vole Clethrionomys glareolus 447135 Bear Ursus americanus 9643 Birds Passeroidea 175121 Brown Trout Salmo trutta 8032 Buffalo Bison bison 9901 Carnivores Vulpes 9625 Cat Felis catus 9685 Catfishes Siluriformes 7995 Cattle Bos taurus 9913 Chicken Gallus gallus 9031 Chimpanzee Pan troglodytes 9598 chinchillas Chinchillidae 10150 Copper Pheasant Syrmaticus soemmerringii 9067 Deer Cervus elaphus 9860 Deer mouse Peromyscus maniculatus 10042 Dog Canis familiaris 9615 Ducks Anas 8835 Ferret Mustela putorius furo 9669 Fish Hyperotreti 117565 Gerbil Gerbillina 10045 Goat Capra hircus 9925 Gray wolf Canis lupus 9612 Guinea pig Cavia porcellus 10141 Hamster Mesocricetus auratus 10036 Horse Equus caballus 9796 Human Homo sapiens 9606 Macaque Macaca fascicularis 9541 Mongolian Gerbil Meriones unguiculatus 10047 Monkey Platyrrhini 9479 Mouse Mus musculus 10090 None None Parrot Psittacidae 9224 Pig Sus scrofa 9823 Rabbit Oryctolagus cuniculus 9986 Rainbow trout Oncorhynchus mykiss 8022 Rat Rattus 10114 Raven Corvus corax 56781 sei whale Balaenoptera borealis 9768 Sheep Ovis aries 9940 Squirrel Spermophilus richardsonii 37591 Tree shrew Tupaiidae 9393 Trouts, salmons & chars Salmoninae 504568 Turkey Meleagris gallopavo 9103 Vole Microtus ochrogaster 79684 Water buffalo Bubalus bubalis 391902 Bovine Rhinotracheitis Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 45H5.20) Texas Vet Lab, Inc. VO_0001877 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 45H5.21) Texas Vet Lab, Inc. VO_0001878 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida-Salmonella Typhimurium Bacterin-Toxoid (USDA: 4109.20) Texas Vet Lab, Inc. VO_0001879 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Parainfluenza 3 Killed Virus Vaccine-Mannheimia Haemolytica-Pasteurella Multocida Bacterin (USDA: 4265.00) Boehringer Ingelheim Vetmedica, Inc. VO_0001885 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 43S5.20) Texas Vet Lab, Inc. VO_0001905 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida-Salmonella Typhimurium Bacterin-Toxoid (USDA: 43T5.20) Texas Vet Lab, Inc. VO_0001906 Inactivated or "killed" vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 45B9.20) Boehringer Ingelheim Vetmedica, Inc. VO_0002006 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus Modified Live Virus Vaccine-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 45B9.21) Boehringer Ingelheim Vetmedica, Inc. VO_0002007 Live, attenuated vaccine Licensed USA Bovine Rhinotracheitis-Virus Diarrhea-Parainfluenza 3-Respiratory Syncytial Virus-Mannheimia Haemolytica-Pasteurella Multocida Modified Live Virus, Avirulent Live Culture Vaccine (USDA: 11A8.22) Intervet Inc. VO_0002008 Live vaccine Licensed USA Bovine Virus Diarrhea Killed Virus Vaccine-Haemophilus Somnus-Mannheimia Haemolytica-Pasteurella Multocida Bacterin-Toxoid (USDA: 4C95.20) Texas Vet Lab, Inc. VO_0002024 Inactivated or "killed" vaccine Licensed USA licensed Pasteurellosis human vaccine Generic Unknown VO_0000620 Inactivated or "killed" vaccine Licensed A generic representation of vaccines utilized to prevent Pasteurellosis (fowl cholera) in humans, most commonly formulated as inactivated or 'killed' vaccines containing Pasteurella multocida antigens. These vaccines are designed to induce protective immunity without the risk of causing disease. Mannheimia Haemolytica-Pasteurella Multocida Avirulent Live Culture Vaccine (USDA: 1861.01) Intervet Inc. VO_0002029 Live vaccine Licensed USA P. multocida DNA vaccine pcDNA-OMPH + pcDNA-OMPA VO_0004536 DNA vaccine Research pcDNA3.1(+) [Ref2654:Gong et al., 2013] Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction DNA vaccine construction A significant increase in serum antibody levels was observed in chickens vaccinated with divalent combination. Additionally, the lymphocyte proliferation (SI value) and the levels of IFN-γ were both higher in chickens immunized with divalent combination than in those vaccinated with monovalent DNA vaccines (P<0.05) (Gong et al., 2013). VO_0000286 The protection provided by divalent combination vaccine was superior to that provided by monovalent DNA vaccines after challenging with the avian P. multocida strain CVCC474 (Gong et al., 2013). P. multocida DNA vaccine pcDNA-OMPH/OMPA VO_0004537 DNA vaccine Research pcDNA3.1(+) [Ref2654:Gong et al., 2013] Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction DNA vaccine construction A significant increase in serum antibody levels was observed in chickens vaccinated with the fusion DNA vaccine. Additionally, the lymphocyte proliferation (SI value) and the levels of IFN-γ were both higher in chickens immunized with the fusion DNA vaccine than in those vaccinated with monovalent DNA vaccines (P<0.05) (Gong et al., 2013). VO_0000286 Protection provided by the fusion DNA vaccine was superior to that provided by monovalent DNA vaccines after challenging with the avian P. multocida strain CVCC474. The protective efficacy in chickens immunized three times with the fusion DNA vaccine was equivalent to the protective efficacy in chickens vaccinated once with the attenuated live vaccine (Gong et al., 2013). P. multocida rOmpH Vaccine VO_0004221 Subunit vaccine Research subcutaneous injection subcutaneous injection 100μg of rOmpH (Tan et al., 2010). Recombinant protein preparation The P. multocida ompH gene was cloned into E. coli and then extracted and purified to result in rOmpH (Tan et al., 2010). Pasteurella multocida aroA mutant vaccine VO_0002862 Live, attenuated vaccine Research Intramuscular injection (i.m.) Intramuscular injection (i.m.) Gene mutation This aroA mutant is from Pasteurella multocida (Dagleish et al., 2007). An aroA mutant is highly attenuated in calves (Dagleish et al., 2007). An aroA mutant induces significant protection in calves from challenge with wild type Pasteurella multocida (Dagleish et al., 2007). After the second vaccination, serum IgM titers rose. Groups receiving 10^9 and 10^8 CFU of the mutant had significantly higher titer of IgM than those receiving 10^7 CFU, and all vaccinated groups had significantly higher titers than the unvaccinated challenge control group of calves (Dagleish et al., 2007). After second vaccination, serum IgG titers rose. Groups receiving 10^9 and 10^8 CFU of the mutant had significantly higher titer of IgG than those receiving 10^7 CFU, and all vaccinated groups had significantly higher titers than the unvaccinated challenge control group of calves (Dagleish et al., 2007). Pasteurella Multocida Avirulent Live Culture, Avian Isolate Vaccine (USDA: 1871.01) Biomune Company VO_0001722 Live vaccine Licensed USA Pasteurella Multocida Avirulent Live Culture, Avian Isolate Vaccine (USDA: 1871.02) Lohmann Animal Health International, Arko Laboratories Ltd., Biomune Company VO_0001723 Live vaccine Licensed USA Pasteurella Multocida Avirulent Live Culture, Avian Isolate Vaccine (USDA: 1871.03) Intervet Inc., Arko Laboratories Ltd., Biomune Company VO_0001724 Live vaccine Licensed USA Pasteurella Multocida Avirulent Live Culture, Avian Isolate Vaccine (USDA: 1871.04) Intervet Inc. VO_0001725 Live vaccine Licensed USA Pasteurella Multocida Avirulent Live Culture, Avian Isolate Vaccine (USDA: 1871.05) Intervet Inc. VO_0001726 Live vaccine Licensed USA Porcine Rotavirus-Transmissible Gastroenteritis Modified Live Virus Vaccine-Bordetella Bronchiseptica-Clostridium Perfringens Type C-Erysipelothrix Rhusiopathiae-Escherichia Coli-Pasteurella Multocida Bacterin-Toxoid (USDA: 49T9.21) Intervet Inc. VO_0002319 Live, attenuated vaccine Licensed USA aroA 581456 CDD:179423 CDD:30129 GOA:Q04570 InterPro:IPR001986 InterPro:IPR006264 InterPro:IPR013792 InterPro:IPR023193 UniProtKB/Swiss-Prot:Q04570 747 ? 441 3-phosphoshikimate 1-carboxyvinyltransferase; Provisional >gi|581456|emb|CAA78480.1| aroA [Pasteurella multocida] MIKDATAITLNPISYIEGEVRLPGSKSLSNRALLLSALAKGKTTLTNLLDSDDVRHMLNALKELGVTYQL SEDKSVCEIEGLGRAFEWQSGLALFLGNAGTAMRPLTAALCLSTPNREGKNEIVLTGEPRMKERPIQHLV DALCQAGAEIQYLEQEGYPPIAIRNTGLKGGRIQIDGSVSSQFLTALLMAAPMAEADTEIEIIGELVSKP YIDITLKMMQTFGVEVENQAYQRFLVKGHQQYQSPHRFLVEGDASSASYFLAAAAIKGKVKVTGVGKNSI QGDRLFADVLEKMGAHITWGDDFIQVEKGNLKGIDMDMNHIPDAAMTIATTALFAEGETVIRNIYNWRVK ETDRLTAMATELRKVGAEVEEGEDFIRIQPLNLAQFQHAELNIHDHRMAMCFALIALSKTSVTILDPSCT AKTFPTFLILFTLNTREVAYR Virmugen An aroA mutant is highly attenuated in calves and induces significant protection from challenge with wild type P. multocida [Ref1721:Dagleish et al., 2007]. IgG Bos taurus 281766 116686102 BOS_2620 GJ060600 NP_001070870 9913 3 7996510 8005231 + transcript variant 2 8.71 25616 250 Also known as CD16; FCGR3; FCGR3@ >gi|258513364:7996510-8005231 Bos taurus breed Hereford chromosome 3, Bos_taurus_UMD_3.1, whole genome shotgun sequence CACTCTAACACACAGCATGTGGCAACTGCTACCACCGGCAGCTCTGCTGCTTCTAGGTAAGTCAGACTCA CTTGGCCTGAGAGCAGAGCAAAGATGCCCTGGGCACAGCAGGCATCTCTTCTCAGGCAAGGAATGAGACC CTTAACCAAGGACAGACCCCTTCCTATGTCTATAGACCTTGGTTTAGCTCCTAGGACACAGCTTCAGGGG TTAGAGATACAGGAAATGGGCATAATCGGGAGGTGAAAGGAGAGGACGTGAGTTCTCCGTCACTTCACTC CTCTAGGTCTCAGCTTCTCTGGCTATAAACTGACAAGTATGCACTGGGTAGCCCCTAAGGTCTGTCTCCC ATTCTCACTTTCCAATGCTCCTCTCTCAGTCGTTTCATTTCTATCCTTTCCAGAGGACTTGGGTGGAGAT AGTTTCCATGGCTTGATTCAGAGATATTCAACCTGTGCCCTTAACTTTGGGGTTTGAACAGGGACCAAGG AGGAAGGGTATTGAAAACAGGGATTTGGGCAGACAACAGATTAGGAGGTACAGACGCTGGAAACCAGAAG GCAGGCAGTGGGGGAGATACCCACTCTATTCATCTTTGTTGAAGATGTCTTTACCTTGTTTGTTTATTTA ATGGCTATACGGGGCCTTCACTGTGGCGCTCAGGCTCTTCATCGCTTTGCGCGGGCTGCCTCTAGTGGCG GAGCACAAGCCTCTTGTCGCGGTGGTCTCTCGTCGCGGTGGTCTCTCTTGTTGTGGAGCACAGGCTCTAG GGTGCTCAGGCTTCAGTAGTTGTGGCTCATGGGCTCTAGAACATGAGCTCTGGTTGCTCTGCAGTGTGTG GGATCTTACTTCTGGCGCCAGGGATTGAACCTGCGTCCCCTTTACTGGCAGGCGGATTCTTACCACCAGG GAAGTCCCCACTATCTTTATCCTGATTCTTGGGAACACTGGCCTCTGAGGGCAGAGGTGGGCAGGGGAGT TTAAATACCACACTCCAGTCACCCTGTTCTCTGCTGCTGACCAATATTTGTTTTATTCTTTTTTTCTTAC AGTTTCAGCTGACACGCAAACTGGTGAGTTTGCTTCATGGTCTTGGACGGATCGAGTGAGGCATGTAAAT GTGTAATAGCTGCAGGGCACTGTTAGGAAATGTTCTTTGCACAAGAAACTGTTGACATGGAGTTGATGGT GGTGGTGGTGGTGGTTTAGTCGCTAAGTTTTGTCTGACTTGCAACCCCATGGACTATAGCCTTCCAGGCT CCTCTGTCCATGGGATTCTCCAGGCAAAATATTGGAGTGGATTGCCATTTCCTTCTCCAGAGGATCTTCC CGACCCAGGGATTGAAACCAGGTCTCTTGCATTGCGGGCAGATTCTTGGCAGGGGCAATTCCCCCAATAC AGTGGGCTTGGCCCTACCAAGGAGGAGGCTAAGTCTCTCCAAGTCAGTATGCTCAGACTGAGTGCAATGC TGCTGCTGCTGCTGCTGCTGCTGCTAAGTCACTTCAGTCGTGTCCGACTCTGTGCGACCCCACAGACGGC AGCCCACCAGGCTCCGCCGTCCCTGGGATTCTCCAGGCAAGAACAGTGGAGTGGGTTGCCATTTCCTTCT CCAATGCGTGAAAGTAAAGTCGCTCAGTCGTGTCCAACTCTTAGCGACCCCATGGACTGCAGCCTACCAG GCTCCTCCATCCATGGGATTTTCCAGGCAAGAGTACTGGAGTGGGTTGTCATTGCGTTCTCCAGACTGAG TGCAATACTAAGGCTGAAATGCCAACTCTCAGTTTGCAATGGGTTTTGAGCCTCCTTGGGAATACCAGTA TAGAGCCTGCTCACTTCTCTGCCCTTATCTACATTCTCTTAACACTTCAGCAGATCCCTCAAAGGCTGTG GTGCTCCTAGACCCTCAGTGGAACCACGTGCTCACGAATGACCGTGTGACTCTGAAGTGCCAGGGAGACT ACCCTGTTGAAGACAATTCCACAAAGTGGTGGCACAATGGGACTCTCATCTCAAGCCAGACCCCCAGCTA CTTCATCGCAGATGTCAAGGTTCAGGACAGTGGCGAGTACAAGTGTCAGACAGGCCTCTCTGCACCCAGT GACCCGGTGAAGCTAGAAGTCCACGTAGGTGAGTAGGAGAAGGGGAAAAAGGAACTGACCAATAAAGGAT AAAAAAAAAAAGAGGCCCTGAAAAAAAATAATGTTCCTGGGGATTAAAACTAGGATGAGATGATCTGTAG TCTATTGAATTACATCAGTATTGTAGTCCTAGCTCCAAGTAGGCCTTAGATAAAATGTCAAAGCCTGGCA TACACTAGGGACAGGGCTGCTGGACTGCACAATTCCAAGAATCTACATTCACATAGAAGATGAATATATA CAGCATGAATGGTGTCATCTGAAGGTCAGGGCATGACATCAATGCTCTATAACAGTTATTGAATAAATTA ATGAATCCACTTTAGGACCTCAATCTCCTCATTCTCAGACACAATCTCCACATTCTCTAAGCTACCCAAC AAGCCACCCCTAATCTAGCATCATATCTATTTCCTCTTCCCATCAACGCTACACTCAAATATACACTTCA GCCTAAATTTGGCAATGTGAAATCTCTCCTAATAGAGATAGATACACGTTCAGCCCGCACATTCATCATG GAACGAAAGTCTTTGTATCTTAACAAAATCCAGGCATTGGATGCAAGGAGAAGTATGTTGAGGCAGAGAT CTCTCCTGGTGTCCCTGAAATCACACCACATAGCCTGGTACCTGCTGTGTCTTGTAAGCTTCACTCTATT TGCATGACTGCCCCATCTCCATAATGTATGTGAGTGGAACAAGCCTGGAGGGAAAGGCGGGTTTAATGAA GGAGGACCTGCAACAAAATTCCACGGACCTGGGTCTCTGAAGCTGATTTTCTGGGCTCCTACACCTGACT GTGTTACTTAGTTGACTTAGTTCTCAGTCTAGCTAGGTGAGAGATGGCCATGCCTGTGTGCCAAGTTGCT TCAGTCATATCCGACTCTTTGTGACCCTATGGACTGTAGCCTGCCAGGCTCCTCTGTCCATGGGATTCTC CAGGCAAGAATACTGGAGTGGATGGCCATGTCTTCCTCCAGGGGATCTTCCCAACCCAGGGATCTAAGCC ACATCTCTTACATCTCGTGCTTTGGCAGGCAGGTTCTTTACCACTAGCACCACCTGGGAATCCCAAGAGA TGGCCACCTGTGACCAAATAAAATGGGGAATTACCAACAGGAAGCAAGAGAAACCTCAAGTCTTGGAGTT GTTAGTTTGTCTTAAACTTGGCTCTAGAAACTGACTTTAGAAGTCTAATTAGTGTGAACAGTTTTAAACT CTGACCAAGAGCATTAAGTTCACCAGGCAAACTCCTGATCCTTCTTTTGGACCCACTTCACTTATCTTCT TCTCTGAGAAGCTTTGCTTACTTTCTTCGGGCCGGATTTCTTTGCTCCATTCTCTTTGGTCATGCAGTCC TTTGTTCTTAGCCTGTTGTAGCACTTATCACCTGGTATTATAATTATTTCTGAGTATATGTGTTTCTACG CCTAGACCACAGGTTCTTTGATGTGTCTCAGTCATCTGGGTGTCCCCAATCTGCATGACATGACATAGTA AGTTCTCAATAAGATATTTGTTTAACTAATGTATATATGAACATTCACTGAGTCCATGACACCCTGAATG GAATAAGAACGTTCTCCCCTCAAAGAAACCAAACTATATGTGTGTGTGTGTGTGTGTGCGTATATGTGTG TATATACAACATAAGTTATGTATATATATGTGTGTGTGTATGTATGTATATATATGATTACATATGTAAC CCCTGTTAATATTTAAGCACATGTTCTTTCTTTTTTTATGAGCATACATATGTTTTATATTCTTTTTAAC TTAACATTCTCTGATAAATGTTTTCTCATGTCATTTTAAAATTTCTCTGCAGTATCGTTTTAAATTATTC AGGCCAACTTCCTCACCATGACTTCAAGTTCTCTGTGTGTGTGAAGTCTGGTTACTTGGCAAGGCAGTTG TGAAAAGAGTTGTTTTGATGATTAACTGTTTCCGTCTTAAAGGCTGTGCTCTGTAATGAAATGGTCTTGC TAAGACACTGTGGCCCCTGAAATTAGCAGTTACCATCAGCACTACCAAGCATGCTTTTCTTTCCTCTCCC TGACTTTTACAAGGAGTTCTCCTTCACAATTTCTGTAGCCACTCCTACGGATACTCAGTAACCTTCTTCC TGACTGGTTGCAACAGTCTTCCAACATTCCTAAAACCGTCTATTCCTTCTGAAGTCTACCTTTCCTTGTA TTCTAATATGATTCTTACTCTTTGATCTAGAATTTACAATGGCCCCTTATCACCTTCCATAGCAAATCCA AGTGTGGACTTTGAGGATGCTGCAGCTTGCTTCTCACCTGCCTCATCACTTAGTCCTTCTGCTCTCACTT TATGCTTTGATCATGTGTGTGTCTGTCCCTCTCTCCCATTAGACAAAAGCTCTTGGAGTGACTTGAGTTC TCATCCAGAGTCCAGTTGTCTTTTTCCCAAAGAAAGTTACATAAGGACGGCGCCGCCCGGTCTGGAGTTC CACTGTCCCCCAGCACCTGGGTGGTGAGCATTCTCTTCAGAAGAGCTTTGTGCTGGGTGCTGAGGCCTCC TGGGCCTGTGTTCTCTTTGTGTTTTGCAGGCTGGCTATTGCTCCAGGTCGCTCAACGGGTGGTAAATGTG GGAAAGCCCATTCGGCTGAAGTGTCACAGCTGGAAGAAAACTCCTGTAGCAAAGGTCCAGTATTTCCGGA ATGGCAGAGGCAAGAAGTATTCTCATGGGAATTCTGACTTCCACATTCCAGAAGCAAAACTTGAACACAG TGGTTCCTACTTCTGCAGGGGCATTATCGGGTCTAAAAACGAGTCTTCAGAGTCTGTGCAGATCACTGTT CAAGGTAAGACATGTGCGGCACTAAAGGGACCAGAGACCCCTGTGCTGGGAATCTTGCATTCACACTGGG GAGATGAGTTGGGAGGAAATTGCTGACAATTCCACAGACTTCTTTACGAGTGTCATTCACTTTGAAACAT GACAGCAGGTGCAAAGGCCACATTTCCTTCCTCATAAATATGCCTGTCCTAACTTACCTCATTCAAACCA TATCCACACTCTTGTGTATCTCTATCGTGCTGGCCCTTTTTTTGCCTGCTCCTGCTCCCCTTTCTCTTCC AAGTCTCAAGTCTTAGCCAAGAGTCTAGCTGGCAGTAACCCCTCACTTTCTCACAGAAAGACCCAGAACC TGTGTTCTGGCTCTGCTGCTGATTAACCATATGACTGTGGCCAGTTAGTCTCTTGAACTCAATTTCCACT TTTGTAACAAAGACAAGCTGACGTTTATTGGGCATTTTTCAGTGTGCTGGACACTGTGCTGAATGCTTCA TTACATGCACTTAATCTGAACACCTGAGAATTAATGCTTTTGAGCTGAGGTGTTGGAAAAGACTCTTGAG AGCCCCTTGGACAGCAAGGACAGTCAATCCTAAAGGAAATCAGTCCTGAATATTCATTGGAAGGACTGAT GCTGAAGCTGAAACTCCAATCCTTTGGCCACCTGATGAGAAGAACTGACTCATTGGAAAAGACCCTGATG CTGGGAAAGATTGAAGCAGAAGAAGAAGGGATGACAGAGGATGAGATGATTGGATGGCCTCACCGACTCA ATGGCCATGAGTTTGAGTGAGCTCTGGGAGTTGGTGATGGACAGGGAAGCCTGGCGTGTTGTAGTCCATG GGGTCACAAAGAGTCGGACACGACTGAGTGACTGAACTGAACTGATATTGAATTCCACTTTCCAAATCAT TTAGAAAACATCTCCAGTTTCTGTAGCACCATCCTCCAGGGTCAGAGATGCCCTGCCATGGTGCTCCTGG ACTTCCTAATCTCCCAAAACTTCCACCTGGAATCAGACTGAGATAAAAACCCTGATACTGGGAAAGATTG AGGGCAGGAAGAGAAGGGGGCGACAGAGGATGAGATGGTTGGATAGTGTCATTGACTAAACGGACATGAG TTTGAGCAAACTCTGAGAGATAGTGGAGGACAGTTAAGCCTGGCATGCTGCAGTCCATGGGGTCACCAAG ATTCGACACGACTGAGTGACTGAACAACAATACACTGTAAGCAATTTACATGTAATAATACAGCAATATA GACACACATCTTCTGAGATTCTAACAAAACAATGCCATAGTTTGTATAAAAAATAACTTCATCACAATTT AAATTGAAAGTAAATACTCTCAAATAGCTAGGAAAAGAGAGAGCAAATAATAAGGAAAATGGGGGCAAAT TGCAAACAATTAGTAAATCTAGGTAAAGAATATCTGAGAGTTATTTGTACTTTATTGACATCCATTTTTT AAGTTCAAAATTATATCAAAATTAAGATTTTTTAAATTGAGACTAAAAATGCACTCTCATCTTACTACCT TATAGGCAACAGCAGAGAATGACTTTAAAACAATCTTAATGGCTAAGAGGTTGAGAAGCAACAAATTCTA GCCCTTTCCATTTTGGGTTCTTAGTTACTGAGCCTTGATAAAGAAATATTTTCTGACGATAAATAGGATA AGTAGTAGATTTCAACGGCTTACCTTGTGTGTGTGTTGGTTTTTGTTTTTGTTTTTTCATTTTCTTGAGT CGGGTGAATACAATGAGTATAATTTTGAGACACAAGAGAAAGTTACTGAATTAAAAAACAGTGTTCTCAC AACAACTTAAACTTTGATATCTCTCATTGCTGTTGTACAGTCACTAAGTCATGTCCGACTTTTTGCAGCC CCATAGACAGCATGCCAGGCTTCCCTGTCCTTACCTAACTCCCAAGTTTGCTCAAACTCATGTCTATTGA GTTAGACATCTCATCCTCTGTCACCTCCTTCTCCTTCGCATCAGGTGGCCAAAGTATTGGAGCTTCAGCT TCAGCATCAGTCCTTCCAATGAATATTCAGGGTTGATGATCTCACGCAAAACCCAGCTACAAGTACCTCC ATCAAACATGAAAACACTATTGCTCAAGAAGCACTAGAATCCAAAACTGGTAGGAGGAAGCTGTGGCTTT CTTCCAGACTCCCCCACATTCAATAGAATCTGTTTCTTAGCAGGTGTCAGAAAGACTTCACAGTGATGAC CTCACTGTTTATGCCAATTTTCTGCCTTAACAAATGTCATTTTTTTCTCATCTCTCTGCTCAGCTCCAGA AACTTTACAAACTGTCTCGTCATTCTTTCTACCTTGGCACCAGATCACCTTCTGTCTGGTGATGGGAGTC CTGTTTGCAGTGGACACGGGACTGTATTTTTCTGTACGGAGACACCTTCAAAGCTCAGAGGAATGGAGGG ATGGCAAAGTCACATGGAGCAAGGGCCCTTAGGACAAATGGAGGTAATGAAAGCAGCATCCCCATCCCAT GGGGTGATAAAAGCAGCATCTATGAACCTCTTCCTAATTTCACAGCCTTATCATCTCCCAACAGGCATCT CTGGGAGCAGCAGGAAAATTACACGTCAGACTCTAGGGATCTGAGGATCCTTCACCCAACTCTGTTTCTT CTAGTCTCCTATGGAAAGAAAGTGCCAACCGTGAAGGACCAGTAAATATCTGCATCACCAGAAATAGAAG TCTCAGAGCTACGTGGCTGCTTTCTGTATTCCAACCATTCTGTCCCAAGAGAACAACCATACAGACTCTC AAAGATCAGCCCTGATGTATGAATATTGTGCTAAAATAAATGGATATGTAAAGAAGAAAATTCATGGTGG AGAGGCAGCTGGATGGTGGTCTACATCTTTTGCTGCACGCTGACTGCCTTCAAGGCACCCTGAAACTCCT TCTAGATAGTCTGTGGGTACATGTGGGGGTGTAGGGAACAGAGCACAACCATTGAAGAGGGTGGCTGAGA AAGAGCAGGGTAGTCCAGGATAGACCAGACAAGACCACCACCTGGGCTGTCTTCCTAGAACATGAACCAT GGTGGAGGTAAAAGAAAATCACAGACGTGAGATGGGATTGAGTTTTCCAGGGGAGCCCCTGTCAAAAGGA TACAGTGTGAGTCTGTGAGTATACAATGATAAATCCTCTCAATGTCATGAGTAGAAAATGATCCAGGAAG GGGGTTAGGGGTTGGAGGATTTGCAAAAGAAAGCACAGAATAAACTCATTGGAAGGAACATTGCTATCCC AAATTAAGCCCACTGAACAAAGGTATATCAGTGTCTAAACCGAGAAAGCATTACAAGAAAAAGTCAACCA CAACTGCACAGGAAGGAGCTGGCAGGAGATGAGGATGCTTTTAGACTGAGAATTAGAGAGAAGGGGCATA TAAAACCAGAATTTTCACTTCAATATCTAATTCAGCTTTATTCACATCACCCTTAAACAAATGTTGGATA ACCACTATTAAATGTACTAAACTCTACTGAATTAAATCATGAAACTTTTAAATCCTTCAGCATGTCAATA ATAATGGAGTAGAGGTGAAATCAATCACTGCTTCTGGAAGAAAGCTTTAGCTATCTCTCTTTGTGTTTTA GAAGCTTTAAACTGAACATTTCTTGATTCCGGTAAGGCATTTTTACAAGATTCTGCATGTTATTGTTAGA TAGAAGATGGAAAAACCACGATAATAAAACATGAATGATAGA >gi|116686102|ref|NP_001070870.1| low affinity immunoglobulin gamma Fc region receptor III isoform 1 precursor [Bos taurus] MWQLLPPAALLLLVSADTQTADPSKAVVLLDPQWNHVLTNDRVTLKCQGDYPVEDNSTKWWHNGTLISSQ TPSYFIADVKVQDSGEYKCQTGLSAPSDPVKLEVHVGWLLLQVAQRVVNVGKPIRLKCHSWKKTPVAKVQ YFRNGRGKKYSHGNSDFHIPEAKLEHSGSYFCRGIIGSKNESSESVQITVQAPETLQTVSSFFLPWHQIT FCLVMGVLFAVDTGLYFSVRRHLQSSEEWRDGKVTWSKGP Vaximmutor IgM Bos taurus 444875 BOS_20600 GJ062732 9913 21 71507508 71508717 + IgM >gi|258513346:71507508-71508717 Bos taurus breed Hereford chromosome 21, Bos_taurus_UMD_3.1, whole genome shotgun sequence AGTGACTTCTGGGAGTGGTGACTCTCATCTGCTCGAAGATGAACCCACTGTGGACCCTCCTCTTTGTGCT GTCAGCCCCCAGAGGTGAGTGTCTCTGGGTCAGACATGGGCACGTGGGGAAGCTGCCTCTGAGCCCACGG GTCACCGTGCTTCTCTCTCTCCACAGGGGTCCTGTCCCAGGTGCAGCTGCGGGAGTCGGGCCCCAGCCTG GTGAAGCCCTCACAGACCCTCTCCCTCACCTGCACGGTCTCTGGATTCTCATTAAGCGATAATAGTGTAG GCTGGGTCCGCCAGGCTCCAGGAAAGGCGCTGGAGTGGCTCGGTGTCATATATAGTGGTGGAAGCACAGG CTATAACCCAGCCCTGAAATCCCGGCTCAGCATCACCAAGGACAACTCCAAGAGCCAAGTCTCTCTATCA CTGAGCAGCGTGACAACTGAGGACACGGCCACATACTACTGTGCAAGAGACACAGTGAGGGGAAGTCAGT GTGAGCCCAGACAAAAACCTCCCTGCACAGGGGCCCGTGACCACGAGGGGGCGCTCAGGACTCAGTACAG AGCTGAGTCCTGGAGCAGGTACGGAAGAGGCGTTGGGCGGTGGGGGTCCCGGGGGGGATTCTCCTCAGAG CTTCTCTTTGCTCCTCCTGCCCAGGAGCTGCAGCATAGACCCATTTCTGTGTTCTGGTATTTCATTGTTG TGGTTTATGTTACTCTCAGACAACTGTGTGTATATATATATTTCATTTTATCTTGAAGCAAGGATAGCTT TAGGAAAACATACAGTCACACATAATACTCAACAGCTAAATTTTTTCTCCTTCATTTTTATTCCTTCAAG GGAACTCAGTAAAACACTTGCTGCTGATACTGCTAAGTCGCTTCAGTTGTGTCCGACTCTGTGTGACCCC ATAGATGCCAGCCCATTAGGCCCCACGGTCCCTGGGATTCTCCAGGCAAGAACACTGGAGTGGGTTGCCA TTTCGTTCTCCAATGCATGAAAGTGAAAAGTGAAAGTGAAGTCGCTCAGTCCTGTCCAACTCTTCACGAC TCCATGGACTGCAGCCTACCAGGCTCCTCCGTCCATGGGATTTTCCAGGCAAGAGTACTGGAGTGGGGTG CCATTGCCTCCTCCGTCAGTAAAACCCTTGACTCTCGATAATTTCAAAACTGTTAACTATCCTGAAAGGA AACATAAGATATTTAAACAT Vaximmutor OmpA Pasteurella multocida strain 95010872 49182347 49182348 AAT57676.1 CDD:236764 CDD:279698 CDD:143586 747 ? OmpA 9.2 34678.17 396 outer membrane protein A; Reviewed >gi|49182347|gb|AY643794.1| Pasteurella multocida strain 95010872 OmpA gene, complete cds ATGAAAAAAACAGCAATTGCATTGACTATCGTTGCACTAGCCGTGGCTTCAGTTGCACAAGCTGCACCAC AACCTAACACATTCTATGTAGGTGCTAAAGCAGGTTGGGCATCTTTCCACGATGGTTTAAATCAAGCTAA ATATCTTGAAGCTCCTGAGGCTACATTTGGCTTTAAACGTAATAGCGTTACTTATGGCGTGTTTGGTGAA TATCAAATCACTGATAATTTTGCAGTAGAGTTAGGCTATGATGATTTTGGTCGTGCGAAGTTACGTATGG CTGAAAAAGATCAAAAAGCAAAAGATGCAGCAAAACACACTAACCATGGTGCGCATTTAAGCTTAAAAGC AAGCTATCCTGTACTTGATGGTTTAGATATTTATGCGCGTGTTGGAGCAGCATTAATTCGCTCAGATTAT AAAGTGTATGATCATTCTGACCCAGCAAAATTACCACAATTTAAGAGAACTCATAGCACCCAAGTTTCTC CAGTATTCGCAGGTGGTTTAGAATATGCGTTTATGCCTGAGTTAGCTTTACGTGTTGAATACCAATGGGT AAACAATGTAGGTAAAGTTAAAGATGTTCTTGGAGAACGCGTAGATTACAGACCAGATATCGGCTCTGTA ACAGCTGGTTTCGGTCAATCTGTTTATGTACCAGAAGTTGTGAGCAAAACATTCACATTAAACTCTGATG TGACATTCGGTTTCGATAAAGCTGACTTAAAACCAGCTGCACAAAACGTGTTAGATGGTATCTACGGTGA AATCGCCCAGTTAAAATCTGCCTCTGTTGCAGTTGCTGGTTACACAGACCGTTTAGGTTCTGATGCATAC AACTTAAAATTATCACAACGTCGTGCTGACACTGTGGCTAACTACTTAGTAGCGAAAGGTGTTGCACAAA ACGCTATCAGTGCAACAGGTCATGGTGAAGCGAACCCTGTAACTGGTAACAAATGTGATTCAGTTAAAGG TCGTAAAGCACTTATCGCATGTTTAGCTGACGATCGTCGCGTTGAAATCGCTGTTAAAGGTAACAAATAA >AAT57676.1 OmpA [Pasteurella multocida] MKKTAIALTIVALAVASVAQAAPQPNTFYVGAKAGWASFHDGLNQAKYLEAPEATFGFKRNSVTYGVFGE YQITDNFAVELGYDDFGRAKLRMAEKDQKAKDAAKHTNHGAHLSLKASYPVLDGLDIYARVGAALIRSDY KVYDHSDPAKLPQFKRTHSTQVSPVFAGGLEYAFMPELALRVEYQWVNNVGKVKDVLGERVDYRPDIGSV TAGFGQSVYVPEVVSKTFTLNSDVTFGFDKADLKPAAQNVLDGIYGEIAQLKSASVAVAGYTDRLGSDAY NLKLSQRRADTVANYLVAKGVAQNAISATGHGEANPVTGNKCDSVKGRKALIACLADDRRVEIAVKGNK Protective antigen DNA vaccines with OmpH and OmpA was superior to that provided by monovalent DNA vaccines.[Ref2654:Gong et al., 2013] ompH Pasteurella multocida strain X-73 2745957 2745958 AAC02243.1 CDD:225744 CDD:238208 747 ? outer membrane protein 8.3 35024.16 419 OmpH >gi|2745957|gb|U50907.1|PMU50907 Pasteurella multocida strain X-73 outer membrane protein (ompH) gene, complete cds AAATATAAATAAAATTTGGGTGAAGTGAGAAATAGAGGCATTTACCCGATGTTTTTATCTTTGATTTATA CTATTTTTGTTTTTAAATCAATGTGTTATGTTTTAAAAGAAAAGTTCTAAAGGAAATAAAATTTTTAATA AATGTGATCTTTTTAACACTTTTTTAACCCTGAAAAAACCGCTGATTTTTTGTAACATGTTGTTTTTAAA GGATAAGGTAAAAAAAAAGACAAACCTATTTTGTTTTGACAAGAAAACGGAGCTTTGCTATCTTGCATTT GCAAAAATGTGCAGACGACTTATCGTCTGTTAGTCGATTTTAGGTTGATTGAGTGATTGGCAACAATCAT TTAATCGAATTATAAAACTCCAAAAACAAGGTAGTGATACTATGAAAAAGACAATCGTAGCATTAGCAGT CGCAGCAGTAGCAGCAACTTCAGCAAACGCAGCAACAGTTTACAATCAAGACGGTACCAAAGTTGATGTA AACGGTTCTTTACGTTTAATCCTTAAAAAAGAAAAAAATGAGCGCGGTGATTTAGTGGATAACGGTTCAC GCGTTTCATTCAAAGCATCTCATGATTTAGGCGAAGGCTTAAGCGCATTAGCTTATACAGAACTTCGTTT TAGTAAAAATGTACCCGTGCAAGTAAAAGACCAACAAGGTGAAGTAGTACGTGAGTATGAGGTTGAGAAA CTTGGTAACAATGTTCACGTAAAACGTCTTTATGCGGGTTTCGCGTATGAAGGTTTAGGTACATTAACAT TCGGTAACCAATTAACTATCGGTGATGATGTTGGTCTATCTGACTATACCTATTTCAACAGTGGTATTAA TAACCTCCTTTCTAGCGGTGAAAAAGCAATTAACTTTAAATCTGCAGAATTCAATGGTTTCACATTTGGT GGTGCGTATGTCTTCTCTGCTGATGCTGACAAACAAGCATTACGTGATGGTCGCGGTTTCGTTGTAGCAG GTTTATACAACAGAAAAATGGGTGATGTTGGTTTTGCATTCGAAGCCGGTTATAGCCAAAAATATGTGAA ACAAGAAGTTGAACAGAATCCGCCAGCAGCACAAAAAGTGTTTAAAGATGAAAAAGAGAAAGCTTTCATG GTGGGTGCTGAGTTATCATATGCTGGTTTAGCGCTTGGTGTTGACTACGCACAATCTAAAGTGACTAACG TAGATGGTAAAAAACGTGCTCTTGAAGTGGGTTTAAATTATGACCTTAACGACAGAGCGAAAGTTTACAC AGACTTCATCTGGGAAAAAGAAGGTCCTAAAGGTGATGTTACAAGAAACCGTACTGTCGCTGTAGGTTTT GGTTACAAACTTCACAAACAAGTGGAAACTTTTGTTGAAGCAGCTTGGGGTAGAGAGAAAGACTCTGATG GTGTAACAACAAAAAACAACGTAGTAGGTACAGGTTTACGCGTACACTTCTAATTTTTGTTAGAATCTGA AAAAAGCCAGTGTTAAACACTGGCTTTTTATTGGGTTTTATTTGTTTTACTTACAATAAATTAGGATTTT GAAAGTCGTTACGCGGTCATCTTTCTCAAAATAATACATATCAATATGTTGCAAGGAATGGATC >AAC02243.1 outer membrane protein [Pasteurella multocida] MKKTIVALAVAAVAATSANAATVYNQDGTKVDVNGSLRLILKKEKNERGDLVDNGSRVSFKASHDLGEGL SALAYTELRFSKNVPVQVKDQQGEVVREYEVEKLGNNVHVKRLYAGFAYEGLGTLTFGNQLTIGDDVGLS DYTYFNSGINNLLSSGEKAINFKSAEFNGFTFGGAYVFSADADKQALRDGRGFVVAGLYNRKMGDVGFAF EAGYSQKYVKQEVEQNPPAAQKVFKDEKEKAFMVGAELSYAGLALGVDYAQSKVTNVDGKKRALEVGLNY DLNDRAKVYTDFIWEKEGPKGDVTRNRTVAVGFGYKLHKQVETFVEAAWGREKDSDGVTTKNNVVGTGLR VHF Protective antigen rOmpH was used in a mice protection study. Mice immunized and challenged intraperitoneally resulted 100% protection against P. multocida whilst mice immunized subcutaneously and challenged intraperitoneally only resulted 80% protection [Ref1802:Tan et al., 2010]. ompH Pasteurella multocida strain Pm-17 99083557 99083558 ABF66676.1 CDD:225744 CDD:238208 747 ? outer membrane protein 9.15 34480.88 413 Outer membrane protein (porin) [Cell wall/membrane/envelope biogenesis]; COG3203 >gi|99083557|gb|DQ417897.1| Pasteurella multocida strain Pm-17 outer membrane protein (ompH) gene, complete cds ATGAAAAAGACAATCGTAGCATTAGCAGTCGCAGCAGTAGCAGCAACTTCAGCAAACGCAGCAACAGTTT ACAATCAAGACGGTACAAAAGTTGATGTAAACGGTTCTGTACGTTTACTTCTTAAAAAAGAAAAAGATAT GCGTGGTGATTTAATTGATAACGGTTCACGCGTTTCTTTCAAAGCATCTCATGACTTAGGTGAAGGCTTA AGCGTATTAGGTTATGCAGAACTTCGTTTTAGTAAAGATGTGAAAGATAAAGAGGGTAAAGTTAACCAAC CAATCGGTAACGAAGTTCATGCTAAACGTCTTTATGCGGGTTTTGCGTATGAAGGTTTAGGTACATTAAC TTTTGGTAACCAATTAACAATTGGTGATGATGTTGGTGTGTCTGATTACACTTATTTTAACAGTGGTATC AATGGCGTACTTATTACTAGTGGTCAAAAAGCAATTAACTTCAAATCAGCAGAGTTCAACGGTTTCACAT TTGGTGGTGCGTATGTGTTCTCAGGCGATGCAAACAAAGATGCATTACGTGATGGTCGCGGTTTCGTAGT AGCAGGTTTATACAACAGACAAATCGGTGATGTTGGTTTTGCATTCGAAGCAGGCTATAGCCAAAAATAT GTAAAACAGAAAGTTGAACAACCTCAGCCTTTACCTCCTGGTCAAGTAGAGCGTTTCAAAGATGAAAAAG AAAAAGCTTTCTTGGTCGGTGCAGAATTATCGTACGCTGGTTTAGCGCTTGGTGTTGACTACGCACAATC TAAAGTGACTAACGTAGATGGTAAAAAACGTGCACTTGAAGTGGGTTTAAACTATGACCTTAATGATAAA GCGAAAGTTTATACAGACTTCATCTGGGAAAAAGAAGGTCCTAAAGGTGATGTTGAAAGAACTCGCACTG TAGCTGTAGGTTTTGGTTACAAACTTCACAAACAAGTTGAAACCTTTGTTGAAGGTGCTTGGGGAAGAAC AAAAGATGCAGATGGTAAAACAACAAAAGATAATGTAGTTGGTACAGGTTTACGCGTACACTTCTAA >ABF66676.1 outer membrane protein [Pasteurella multocida] MKKTIVALAVAAVAATSANAATVYNQDGTKVDVNGSVRLLLKKEKDMRGDLIDNGSRVSFKASHDLGEGL SVLGYAELRFSKDVKDKEGKVNQPIGNEVHAKRLYAGFAYEGLGTLTFGNQLTIGDDVGVSDYTYFNSGI NGVLITSGQKAINFKSAEFNGFTFGGAYVFSGDANKDALRDGRGFVVAGLYNRQIGDVGFAFEAGYSQKY VKQKVEQPQPLPPGQVERFKDEKEKAFLVGAELSYAGLALGVDYAQSKVTNVDGKKRALEVGLNYDLNDK AKVYTDFIWEKEGPKGDVERTRTVAVGFGYKLHKQVETFVEGAWGRTKDADGKTTKDNVVGTGLRVHF Protective antigen DNA vaccines with OmpH and OmpA was superior to that provided by monovalent DNA vaccines.[Ref2654:Gong et al., 2013] Dagleish et al., 2007 journal Dagleish MP, Hodgson JC, Ataei S, Finucane A, Finlayson J, Sales J, Parton R, Coote JG 2007 75 12 5837-5844 Infection and immunity Gong et al., 2013 journal Gong Q, Qu N, Niu M, Qin C, Cheng M, Sun X, Zhang A 2013 152 3-4 317-324 Veterinary immunology and immunopathology Harper et al., 2006 journal Harper M, Boyce JD, Adler B 2006 265 1 1-10 FEMS microbiology letters Tan et al., 2010 journal Tan HY, Nagoor NH, Sekaran SD 2010 27 3 430-441 Tropical biomedicine Wiki: P. multocida website http://en.wikipedia.org/wiki/Pasteurella_multocida