Gallid herpesvirus 1 10386 Avian infectious laryngotracheitis, Fowl Laryngotracheitis ILTV infection of chickens frequently induces production of virus-neutralizing antibodies, but antibody titers correlate only poorly with protective immunity (Fuchs et al., 2007). Like other avian herpesviruses, ILTV exhibits a very narrow host range both in vivo and in vitro. Besides domestic chickens, pheasants represent the only natural hosts. Furthermore, the virus could be isolated from peafowl, and experimental infections of turkeys have also been described (Fuchs et al., 2007). Infectious laryngotracheitis (ILT) is a worldwide occurring respiratory disease of chickens, which is caused by a virus designated as ILTV or gallid herpesvirus 1 (GaHV-1). Infectious laryngotracheitis virus (ILTV) is an alphaherpesvirus that causes an economically important chicken disease, which results in delayed growth, reduced egg production, and also frequently in death of the animals. After acute infection of the upper respiratory tract, the virus can establish latency in the central nervous system, and subsequent reactivations can lead to infection of naive chickens. For prevention of ILT, conventionally attenuated live vaccines are available. The severity of clinical symptoms depends on the virulence of a particular strain or isolate, and mortality rates can vary between 0 and more than 70%. Symptoms of milder forms of ILT are nasal discharge, conjunctivitis and reduced egg production, whereas severe forms are additionally characterized by gasping, coughing, expectoration of bloody mucus, and marked dyspnoea which may lead to suffocation. Pathomorphological alterations can be observed in conjunctiva, larynx, trachea, and sometimes also in the lungs and air sacs (Fuchs et al., 2007). Baboon Papio cynocephalus 9556 Bank vole Clethrionomys glareolus 447135 Bear Ursus americanus 9643 Birds Passeroidea 175121 Brown Trout Salmo trutta 8032 Buffalo Bison bison 9901 Carnivores Vulpes 9625 Cat Felis catus 9685 Catfishes Siluriformes 7995 Cattle Bos taurus 9913 Chicken Gallus gallus 9031 Chimpanzee Pan troglodytes 9598 chinchillas Chinchillidae 10150 Copper Pheasant Syrmaticus soemmerringii 9067 Deer Cervus elaphus 9860 Deer mouse Peromyscus maniculatus 10042 Dog Canis familiaris 9615 Ducks Anas 8835 Ferret Mustela putorius furo 9669 Fish Hyperotreti 117565 Gerbil Gerbillina 10045 Goat Capra hircus 9925 Gray wolf Canis lupus 9612 Guinea pig Cavia porcellus 10141 Hamster Mesocricetus auratus 10036 Horse Equus caballus 9796 Human Homo sapiens 9606 Macaque Macaca fascicularis 9541 Mongolian Gerbil Meriones unguiculatus 10047 Monkey Platyrrhini 9479 Mouse Mus musculus 10090 None None Parrot Psittacidae 9224 Pig Sus scrofa 9823 Rabbit Oryctolagus cuniculus 9986 Rainbow trout Oncorhynchus mykiss 8022 Rat Rattus 10114 Raven Corvus corax 56781 sei whale Balaenoptera borealis 9768 Sheep Ovis aries 9940 Squirrel Spermophilus richardsonii 37591 Tree shrew Tupaiidae 9393 Trouts, salmons & chars Salmoninae 504568 Turkey Meleagris gallopavo 9103 Vole Microtus ochrogaster 79684 Water buffalo Bubalus bubalis 391902 Avian Encephalomyelitis-Fowl Pox-Laryngotracheitis Live Virus, Live Fowl Pox Vector Vaccine (USDA: 10S1.R0) Biomune Company VO_0002038 Live vaccine Licensed USA Fowl Laryngotracheitis Modified Live Virus Vaccine (USDA: 1601.01) Intervet Inc. VO_0001671 Live, attenuated vaccine Licensed USA Fowl Laryngotracheitis Modified Live Virus Vaccine (USDA: 1601.02) Intervet Inc. VO_0001672 Live, attenuated vaccine Licensed USA Fowl Laryngotracheitis Modified Live Virus Vaccine (USDA: 1601.11) Wyeth, Lohmann Animal Health International, Merial, Inc. VO_0001673 Live, attenuated vaccine Licensed USA Fowl Laryngotracheitis Modified Live Virus Vaccine (USDA: 1601.13) Intervet Inc. VO_0001674 Live, attenuated vaccine Licensed USA Fowl Laryngotracheitis Modified Live Virus Vaccine (USDA: 1601.14) Intervet Inc. VO_0001675 Live, attenuated vaccine Licensed USA Fowl Laryngotracheitis Modified Live Virus Vaccine (USDA: 1601.15) Intervet Inc. VO_0001676 Live, attenuated vaccine Licensed USA Fowl Laryngotracheitis-Marek's Disease Serotype 3, Live Marek's Disease Vector Vaccine (USDA: 16J1.R1) Intervet Inc. VO_0002141 Live vaccine Licensed USA Fowl Laryngotracheitis-Marek's Disease Serotypes 2 & 3, Modified Live & Live Marek's Disease Vector Vaccine (USDA: 16J1.R2) Intervet Inc. VO_0002142 Live vaccine Licensed USA Fowl Pox-Laryngotracheitis Live Fowl Pox Vector Vaccine (USDA: 1F31.R0) Biomune Company VO_0002202 Live vaccine Licensed USA Gallid herpesvirus 1 UL0 mutant vaccine VO_0002966 Live, attenuated vaccine Research Eye drop Eye drop Gene mutation This UL0 mutant is from Gallid herpesvirus 1 (Veits et al., 2003). A UL0 mutant is attenuated in chickens (Veits et al., 2003). A UL0 mutant induces significant protection in chickens from challenge with wild type gallid herpesvirus 1 (Veits et al., 2003). Gallid herpesvirus 1 UL23 mutant vaccine VO_0002967 Live, attenuated vaccine Research Intraocular immunization Intraocular immunization Gene mutation This UL23 mutant is from Gallid herpesvirus 1 (Han et al., 2002). A UL23 mutant is attenuated in chickens (Han et al., 2002). A UL23 mutant induces significant protection in chickens from challenge with wild type GHV-1 (Han et al., 2002). Gallid herpesvirus 1 UL47 mutant vaccine VO_0002968 Live, attenuated vaccine Research Intratracheal immunization Intratracheal immunization Gene mutation This UL47 mutant is from Gallid herpesvirus 1 (Helferich et al., 2007). A UL47 mutant is attenuated in chickens (Helferich et al., 2007). A UL47 mutant induces significant protection in chickens from challenge with wild type GHV-1 (Helferich et al., 2007). Laryngotracheitis DNA Vaccine encoding gB VO_0004565 DNA vaccine Research pcDNA3.1(+) [Ref2691:Chen et al., 2011] Intramuscular injection (i.m.) Intramuscular injection (i.m.) DNA vaccine construction Compared with the chickens in the pcDNA3.1(+) and PBS group, the chickens in the pgB with or without pIL-18 groups showed significantly increased levels of anti-ILTV antibodies with subsequent vaccination. In addition, an enhanced T-cell proliferative response to gB was clearly observed in the groups immunized with pgB alone or with pgB plus pIL-18 (Chen et al., 2011). At 21 days of age, the chickens were randomly divided into four groups of 20 chickens each. Chickens in group 1 were administered 150 μg plasmid pgB alone by intramuscular injection into the quadriceps muscle. Chickens in group 2 were immunized with 150 μg plasmid pgB plus 150 μg plasmid pIL-18. Chickens in group 3, which served as a negative-control group, were immunized with 150 μg plasmid pcDNA3.1(+). Chickens in group 4 were immunized with 0.5 ml sterile PBS alone as a blank control (Chen et al., 2011). VO_0003057 The protection rates in the pgB and pgB plus pIL-18 groups were 60 and 80 %, respectively (Chen et al., 2011). At 15 days after the booster immunization, all of the chickens were challenged intratracheally with 150 EID50 virulent ILTV CG strain (Chen et al., 2011). gB Gallid herpesvirus 1 isolate CG 110676569 CDD:223014 10386 ? glycoprotein B 6.87 93635.88 942 glycoprotein BALF4; Provisional >ABG85153.1 glycoprotein B [Gallid alphaherpesvirus 1] MASLKMLICVCVAILIPSTLSQDSHGIAGIIDPRDTASMDVGKISFPEAIGSGAPKEPQIRNRIFACSSP TGASVARLAQPRHCHRHADSTNMTEGIAVVFKQNIAPYVFNVTLYYKHITTVTTWALFSRPQITNEYVTR VPIDYHEIVRIDRSGECSSKATYHKNFMFFEAYDNDEAEKKLPLVPSLLRSTVSKAFHTTNFTKRHQTLG YRTSTSVDCVVEYLQARSVYPYDYFGMATGDTVEISPFYTKNTTGPRRHSVYRDYRFLEIANYQVRDLET GQIRPPKKRNFLTDEQFTIGWDAMEEKESVCTLSKWIEVPEAVRVSYKNSYHFSLKDMTMTFSSGKQPFN ISRLHLAECVPTIASEAIDGIFARKYSSTHVRSGDIEYYLGSGGFLIAFQKLMSHGLAEMHLEEAQRQNH LPRGRERRQAAGRRTASLQSGPQGDRITTHSSATFAMLQFAYDKIQAHVNELIGNLLEAWCELQNHQLIV WHEMKKLNPNSLMTSLFGQPVSARLLGDIVAVSKCIETPIENIRMQDSMRMPGDPTMCYTRPVLIFRYSS SPESQFSTNSTENHNLDILGQLGEHNEILQGRNLIEPCMINHRRYFLLGENYLLYEDYTFVRQVNASEIE EVSIFINLNATILEDLDFVPVEVYTREELRDTGTLNYDDVVRYQNIYNKRFRDIDTVIRGDRGDAIFRAI ADFFGNTLGEVGKALGTVVMTAAAAVISTVSGIASFLSNPFAALGIGIAVVVSIILGLLAFKYVMNLKSN PVQVLFPGAVPPAGTPPRPSRRYYKDEEEVEEDSDEDDRILATRVLKGLELLHKDEQKARRQKARFSAFA KNMRNLFRRKPRTKEDDYPLLEYPSWAEESEDE Protective antigen UL0 Gallid herpesvirus 1 3239037 148828637 GaHV1_gp60 X97256 YP_182395 10386 106974 109923 - protein UL0 6.59 53811.98 506 UL0 family >gi|57790931:106974-109923 Gallid herpesvirus 1, complete genome ATTTATTGTTTATCTCGCAATTTTACATATATTTTAACAGCAACAAGCTCCGCCCCAAAATAAACACCGG CGGAAAAAACATACTAATCTAAATTCCAGTCAATCGGTGACAGGCCCTTAGATTCGAAGTATTGATTTGC TTCCTCGAAATGGCGACAGTTGAACGGACGTCGCGTGCAGGTAGAAGGATGGATGAACTCCAACTTTAAA TGATTTTTAGGCACGCGGGGAATAAATCGTCTAGCTTCTTGGCCCCAAAGCATCAACACCGTAGATGACA ACTTTTCTAGTAGCGATGAGATCACTCCCTTTGTAAATTTGTCCCAGCCGATGGTTGAGTGCGATCTAGG AAAACCTTGTTCCACCGTCAAGTATTTGTTCAAAAGCAATACTCCCTTCCTGGCCCAAGATTCTAGACAC CCGTGAGTTTCCTTTGGAATAGATGGGTAACAGTCGCGTAGAGCTTCCAAAATGCGGCGCAAGCTGGGCG GTATACGGCACCCCCTAGGGACACTGAAAGCTAAGCCATGCGCGTGTCCTTCCGTGGGATACGGGTCTTG CCCAACGATAATAACACGCACATCTTGCGGATTGCAGTATCTTGTCCATGCAAAAATATCTCCTTTAGGA GGAAAGATTTTGCGGAATCTAGAACAGTAGGAGTACTGTAACAGCGTCTCACCGAAATCTTCATTTTCAA TTAGAGGTTTCAGAATTGACTCCCATGATGATGGTATTGAAAACTCTATGGCCACGTCGGTCCACTCCGG TACGAGCTTCCTCGCGGATGGTATAACCAATTGGACGGAAGTCATGCTCGGAGGGGGGAGAGTACGGCGT TTTTTAGCAGCGTTGGGGTCGGTATCTCTTTGGTTGCTTTCAGTAGGAGAGCTAATGTCCAGCGGACGCT TGAAGGGACTTTTTATGGGCTGCTCGCATAGTCCGGGTGATAGTGGTTGATGTTCGTTTCCGGCCATCTG AAATTTATGGTGGTCCTTTTATACAACGAATCAACAAGTCCACGAGCCAAGACGTTAGCAGCCGCAAGCC CGGTAGCTTCCAGCCGGTCTTGAAGAAGTCTAATCCCCAAGTACGGATTCACTTTTGCAGCGTACCCATT CTTATAGTAGAAAACAACTTCCGTGTTTACGCACAGAGGTCTGTAAATAATCACAGTCAAATTTTCAAAG TAGTCTCCCGATGGCAACGGCCCGAAAATTCGCGCACCGTTATCTCGCGAATTACTATCACATGGTGCGG CCGCGGCAGAAACAGGATCACGAAAAATGCCAGGTATATCGACACTTGAACATAGCATAAGAGATGCTAT TAGAAAAAAACAAGTCAACATTTTAAAAATCATTATCATATGGAGACCTAGGTCTCCGTTCACGATCCGT GTTACGTCGACGAGCAGATGGAACTCTTTGCGCCCGACTTCTAAATGGATCGTCGTCAGAATCTGAGTCG TCCGAATACAAATGTAGTGGTCTACAGGAACCAGTAGGTACTCTTTGTGGCGGACTTTTGAATGGATCGT CGTCCGAATCAGAGTCGTCCCAATATAAATATGACGGCCTAGATGGAGTACTTCGGCTCTGTAGTCCTGA AGGACCTTCACCTGCGTCATCGTTTCGCATGCGGAGGCTCCGATTCTGCATGGAATCGAGGTCTAAAATA CCTCTGTCTGCGGCGACGGGGGATTCGGGTTCATTCTTTATCCCCCCATGACGAGTCTGTGCACCAGTCG TATTTTGCCCTGCGCCATCATAATTAGACGAGGGGTGGTGGGGCGAAAGCTGTGGAGGGGAGCGTCTAGA TGGCGAGGGGAGGGATGATAACCTGAGGTCTAGGGCCGTAGGAGATGGCGCAGAAAATAAAGGGGGTATA GAGGACGGACGTGCGGGCGGTGGACTATGAGCGGGGCTATTTTCTATTTCATGCCGCACGACCCGTCGCA CCCACGACATCTCATATACATCTCGCACTTCTGCTCTACTCGCAAGGCCATAGTTGCCTTCCCCCTCTTC TGTCTCCGGTAACATAAACGTATCGGATACGGTGGTCGGCCGCTTTTTAACCATTGGATGTGCGAGCCCA ACTGGCTTGACGTCCGTGCTGTCATGAGACTCGCCTTTGACAGGAAGGCGGATCGACACCATCCCGGCGG AATTTTTAAAATATTTCAAATCCACCGTGAGTGGTCTGGTCAGCATGTCGGGCTGTATCGTCTCAGGAAT GGCCATTCCTACCCTGTACTCTAGACCCGATGCACTCTGTACCGTCGGGGTTTGTTCGTACGCCAAAAAG CTATCATATCCGCTAGGGTGATTCGTCGTAAACTCACGTATCCATCCATTGATCCTGGTTAGAAACGAGT AAAATACTTCAAGCATCTGTTCTTCATCCTGGAATGACAATTCCACTGCGCGTAGTGCGGAATTTGCTCC GCGCGTCACCAGATGACGCGCGTTTGAGATTTCTGGAGCTGAGGCGATTGCCATACTATAAACGACACTT TTAGCCCTCAGTAGAGTAGAATACAGCGCCTCAAAATTTCTCCACGTTAGTTGACGAGTTACAAACGTTT CAAGCATTATCGCCCTCAAAACGACTACAACGTCATCTATGGGGGATAGCCTTCCGGTATGGTGTCTTCG AGTTACTGGTCTTTCCTGTTCCAAAATAGTCTGGATGGCTTGGCGTTCTCCATCGGTAATAGGGGTACCG GCCTGCCGCCTGGTTTGAAGTAATCTCCTAACGCTTTCAACTGTTGTCGGAAAATAAGCTACAACAGAAA CCAAACCATTAATCCTCGTACCTCAAAAAATCTTACACGCGTCATCCCACCCCACAAACCACTTACGCCT CATCAAGACCCAGAAATCCATATAGTGCGCTTCATCGATGTCCGCCACGAATGCTATATTGAAATCTCCG TTCATTGTCA >gi|148828637|ref|YP_182395.2| protein UL0 [Gallid herpesvirus 1] MTMNGDFNIAFVADIDEAHYMDFWVLMRPYFPTTVESVRRLLQTRRQAGTPITDGERQAIQTILEQERPV TRRHHTGRLSPIDDVVVVLRAIMLETFVTRQLTWRNFEALYSTLLRAKSVVYSMAIASAPEISNARHLVT RGANSALRAVELSFQDEEQMLEVFYSFLTRINGWIREFTTNHPSGYDSFLAYEQTPTVQSASGLEYRVGM AIPETIQPDMLTRPLTVDLKYFKNSAGMVSIRLPVKGESHDSTDVKPVGLAHPMVKKRPTTVSDTFMLPE TEEGEGNYGLASRAEVRDVYEMSWVRRVVRHEIENSPAHSPPPARPSSIPPLFSAPSPTALDLRLSSLPS PSRRSPPQLSPHHPSSNYDGAGQNTTGAQTRHGGIKNEPESPVAADRGILDLDSMQNRSLRMRNDDAGEG PSGLQSRSTPSRPSYLYWDDSDSDDDPFKSPPQRVPTGSCRPLHLYSDDSDSDDDPFRSRAQRVPSARRR NTDRERRPRSPYDNDF Virmugen A UL0 mutant in infectious laryngotracheitis virus (ILTV) was attenuated. Chickens immunized with UL0-negative ILTV were protected from clinical disease after subsequent infection with virulent ILTV. [Ref1975:Veits et al., 2003]. UL23 Gallid herpesvirus 1 3239035 57790948 GaHV1_gp17 L36139 YP_182352 10386 29808 34666 - thymidine kinase 5.45 37832.47 364 2.7.1.21 >gi|57790931:29808-34666 Gallid herpesvirus 1, complete genome GTTTATTGATGTTTATGTTTCGGGTAATTATCCAAACAGATCAGGCCGCGTCGCAATTCCCATGTTCTTC CATGCTTCGGACACTGCTGTGCAAGCAGCCGTCTTAAAATCGCCTATCTCATCGGGGGTCCAATGAGAGT GCAATAAAATGCCGAGTCTAGGTGGATCGTACGCGTGGCAAATTGCTGCGCACAGAAACTTGCCGCACAT GGGAGTTTCTTCTAAAAGAGCGCGCCAGAAGGTAAAGCCTCGCCCAGTAAGCCAGCTAGTAGCTCTTGAT GCCCATGTATCTACCGCTAGATATGAGCGTTCGTCCGATGCCAATACCCCCGAAACTGGGAAAAACGCGC AAAGAATGTGAGTTCCTTTCGGAATCGAGGCTGTCTGCTGTGCGCGCGGTGGATGTATTTGGCTGTCGCC AATCGCAACACAAAACGGGATACGGAAAGCCCATTCTTCGCGGCAAGTAATTATCGGCGAAGCTATCTGT GATCGATACGCGCGTAACCCAGTAAATATCGCCGCTTGTAGTAGTCCGACGGGCGAGTGATGCGGGCTGT CTCCGTGCTTTAAATCAAGGATTTCTCCGCTTAGAGTAAGTGATGGAGGACTTTGAAAGAACAAGGCCCC GCTGAATGCGCGTACAGCAGCAACAACCGGAGAAAGTTCCAAATCCGAGATAAGTCCTAACATTAGAAAT CCGACTCCATCTTTCCACTTGTCTGCAGACAACAGTTTAAAAGGCAAATACTTGGCTCCAAAAGTCATCG GGTTTTTTGAATGCACGCTATCAGCTACGGCTTGCCATCTTTGGAAATTTTTTAAGGAAGCTCTTTTCAT GGCAAGAAGGAGCTGCTCGACGCCAATCTCTAAATTAGAATTAGTTGTGTAGGGAGTTCTAGTCATGATA ATTGCAGTAGAAGGAATTCCAGTGTCGTTGGAAAGGGATTCTTGCGGATCCTCAAATAATGGTGCTGCTA CGAATCGGATTTGAGAGTTATCGGGCTGTTTTGAGTCATGGTCTTGAAACACATGAATAGTCAAATCCTT GTTGCAATCCAGAAAAAACGATGCTGTGCCGTGCAGTACAGCTAGGCCATATTCTACCTTTTCTGTCCAT AGTTTTCCACGATGTGTTCCTAATTCCATGTGAGACGAGTCTACAAAGTTTGCGAGGCAGTGAGTTACTG CAAAACGACATTCGCGATTGCTGGTAGACAGTGCTTTGGAAGCCTGAGACTGCTTCTTTGACGGAGCCAT AGCCAGTTATTCGTGCTGCCGCAAGTGCAGTTGAAAAGGGTTTGTAAGGAACAAGGATTAAAGTTCGCTC TCCTCGTCAGACGGTAGCCGTACACGTCTAAATTGCGTTGCCGAGCTGAGGCAGAATTTATATATAGCCC TGATGGCACTCCATAGAGCAACCAATCCAACAAGTGCCCCTACCACAATACCGATAATGTATGCCTTGTC AAAAACTATTATGCTTCTGTCAAGTCCTAAAATTCGGACAGCAGTTCCGTGTGGTCCTAGAAGCGCGTAG TTATAGAACTCGGGGTTCATAGACTTAAAAAATGCAATAGATGAGTTGGCACCGGCTAACAGTTCTGATT GAACATATGCACTGTCTATAAAGAAAGCATCCTGGAACCCCCGTAGACCGTATTGAACTATCAGGTGGTT GCAATATATGCAAGTTTTCGCTCGTCCATTCAAATGACCATCCTCCATTTTTGCTGTTCGCGCGATTTGC GGTAGGGAGTTGCAACTTGAATTCACAAACGTTACAAACAAAGGGGCAGCTGTTGAGGTATCCGGAAGTC TGTAGGTCATTCCAGTGCTCGGCGGGCTACTTGTCATCACCCAACTAGAATGCGCGGTAACAGGGAGGAA AAATACGATGTGGTGTTCCAGGTCCCAGTTTAAACATTTCTTTACCTCTGGAAAAAACGCAGTTGCCAGG ATCTGAGGGGTCAACAGAGCTTGTGGCGGTATCGGTGCACTATATGATGGCAATTCACCTTCAGATGATA AATATATTTTGGTGTAAAAAAATCGAAATGGAGCAGATGCTGTCAGAATTATTTTCCATGGTGCACTTCT GGAAATATCAAACCTTGTCGCAATTGCACAAGGAGAAAACCACTCTTCAATTTTCGATGCCTGTCCTTTC TTGAAGCTCCCGTCTGGTTGGGAGTGAATCTCGTTGATATCGGCTAGCCGGTCGACAGAGCATAAAATTG TCATGCGCCTGAGGAAGTTCCTTCCATGTTGTATAGAAAGTTGAGGAGCCCGCAAAATAATGTACGTAGA AACTGCCAATAACTTTCTGTACGCGGGGGTCATTACCTTTTGTGTAATGATTTCTCTGTAACTGTTATCT ATTATTTCAAGAGCCAGTTGGCTGGGGGTCACTCCATCGATGCTCGCCAGGAATGCATTTTGGGGATCTT GTTCTGCAGTGATAAGCACCGCATTCATGATAGTAAGAAGCAGGTTATGATTTTTTATCATGGGTTCCCA TGGCCTGTCGTGCATAATTTTAAGAATACTGGATGGGATAGAAGTAAGAGCACCACCGACATTCTGAATA AAGCGAATATTCGTTGTATAGTTTACTGAATTCGCCCCCCCAAAACACACTGCGACTTGGTCTGCCCATG TTTGCGTTTCTGCAATAAACTCGACGATCTCCGAATCAGTTACGTATGCATCGATCGTAAACTTTAGAAA TCGAAACCACGCGGCCATAAACCGTGCTGCGAATCTTACAAAAAAAGACTCGTCTATGGTATTCGGGCAA CCTGCGATTGCGACCTTCTGCAAAGTTAAAAAGTGTCCCGCGGATATTTGTTCGTCGAAGCTGGCCAGCT CTAAGAAATCTTGAGCAGCGGTCGTTACTGCTGTAAACAAATTTGGCCGATAATTTGGTCCAGCTGCGAC GTTGATGCGCACAGTTAACCCCTGATCTTCAGCCTTCAGCATATAGGGCCATGACTTCAGGGACGTTTCT TTAACCTTCGCGCTGGTTGGTGTAATTATCATCTCTATTTGAGGAGAATGCTGGGTCGCGATTATAAGGG TCGCCACATTCAGTCCGATGTTAATCTTAAGATCTATGTCACTAGTAACTGCGAGGCTAATATATCGTGT AAACACAAATGGAAAAACAGTTTGTAAAGGATCACTGTAAATTACACGAATCGGAGCGATGATGCTCTCG CCCCTATGAACTGAGTTATTTGGTGGAAAGTTGACGAGTTTCGCAGATGTAAGTGCATTCCCCTTGTATC CTACCAAAAGTCGATGCTCAAGCTTTGGATCGTTGCTGAATTTTCGTGGTTGGATGGTAGCCTCGCTAGC AGAACCCCATCTCGAGTAAGTATAGGCTACGTACAGTTGTCCAAGCAAATCTAGAGCGATCGATAAATTC CTGTTAGCGCTTCCAGGAGGCCCAAAGATTAAGTTTCGATCGACAAGGCATAGTTGTGTGATGTGGCCGT TTTCGACTGCTGTCGCTATCGCAATGAATTCTTTCGGAGGGGCCAAATTTTCACTGGTGGGGGGCACGAA GACAATTTTTGCCTTTGCCTTGTCATCCCCTTTAGCTCTAAGTAATATCGCCAGAATGAGGAAAAATATT TCTCTCGCGTGTCCCATCGGCGCGGGTTGCTGGTGAAGCCACGCTCTCTCGAGTAAGAATGAGTACAAAG CAAGGAAATGAGTAAAGGACATACTCTTATTGTGAAGGATTATGGAAGCGGAACATTACGAACCCATATC AGCATTGTAGCGCTCTACTGCGTCAATTAAGCTCGCGATCGCCGCTCGTTTGGTTGTAAGAGAACTTGCC GTCTCGGCAAACTCTAAACATTTCAGTGCACATGCCTCCGGGGTGAGGCCATGTGCTGGTAAGTAAAAAA TGCACAAGTTTTTCAATACCGTCGCGAGTTTTTGAACCACAAATGAAAGTACAGTTTTTAATCTTTCCCC GTGAACATTTTGCTGATCAAAAGCTAATATTGCAAATAGCGTCTGGTCGATTGAAGGATTGATTTCTGGA ACAGTAGCAGTTTCTAAGCACTCTCTCTTGAATTTTAAGAGCGTGGCACTTGTTAAGGCTTCTTGCTCCT TCAACAATGCTGGATAAGATGTACGTTGGAGGTAGGTGGTAGTATTCATGAGGCAGATGTACGAGTTACG TAATGCACACAAATACGTCCAGTCGACCGTTTCCCCTGGGCGCCCCCTTTGTATTATACGCCGTGCCTGT TCGCAAATATCCAAATCCATTAAAACAATGTTGGAGTGTGTTGGAATGTCAGGAATTCCTGACAATAAAG TTATTAAATCTCCAAATTCGAGCGCTCCGCTTAGATATTGTGCAACAGGGAAACACAAACATGCCGCGAG TGGGTGCCTATCTACGAGGAATAAGACATCATCTGGTCCGGATGCAGCTGCGCTGTAGCAGCGTTCAGAT CCCAAATAATTATAAATGACGTGGTTCATTATTCTTAGTGGGGCAGCAATAGTCATCTGAACTTCCGCTA CGGCAACCGGAGACGCCTCCCGACATTCAAGTTTTCCGGAAGCCTTTAGGGTTTGTATTTCATTAATTTC TTTGATCATATCCGCTCCGCCAAACCATTGGCGCCATGCTTGCATAGGTTCCGCTAAGTATAGACGGCAA CCTCTCCAATTTGGCATTTGCATCAACGTCTTTGCAGTGACTGTTTTACCGACTCCGAATGGCCCGTCGA CGTAAAGAAGTACAACGCGGCGAAAGTCGGAGTCATCGTTCTCGAACTCGGAGCAAAACTGCACACCACC GGAAGTTTTCACGGCGCCAGCTACGGCCA >gi|57790948|ref|YP_182352.1| thymidine kinase [Gallid herpesvirus 1] MAVAGAVKTSGGVQFCSEFENDDSDFRRVVLLYVDGPFGVGKTVTAKTLMQMPNWRGCRLYLAEPMQAWR QWFGGADMIKEINEIQTLKASGKLECREASPVAVAEVQMTIAAPLRIMNHVIYNYLGSERCYSAAASGPD DVLFLVDRHPLAACLCFPVAQYLSGALEFGDLITLLSGIPDIPTHSNIVLMDLDICEQARRIIQRGRPGE TVDWTYLCALRNSYICLMNTTTYLQRTSYPALLKEQEALTSATLLKFKRECLETATVPEINPSIDQTLFA ILAFDQQNVHGERLKTVLSFVVQKLATVLKNLCIFYLPAHGLTPEACALKCLEFAETASSLTTKRAAIAS LIDAVERYNADMGS Virmugen A thymidine-kinase mutant is attenuated in chickens and induced protection from challenge with wild type GHV-1 [Ref1985:Han et al., 2002]. UL47 Gallid herpesvirus 1 3239064 57790998 GaHV1_gp67 EU104855 YP_182402 10386 126615 129614 + tegument protein VP13/14 9.89 65621.89 623 >gi|57790931:126615-129614 Gallid herpesvirus 1, complete genome AATGACCTTGCCCCATCGATTAACGAAAAGACCTTTCGCGCGTCGATTCTGCTCGGTCTTTGTGATACAT TATAGTGAGACTAAACTCGACCGATATAACAAGACAATGTTACTCTATAGACCGGACTCAACCATGCGGC ATAGCGGAGGCGACGCAAATCACAGAGGGATAAGGCCGAGGCGGAAATCTATTGGAGCGTTTAGCGCGCG CGAAAAGACTGGAAAACGAAATGCGCTGACGGAAAGCAGCTCCTCCTCCGACATGCTAGATCCGTTTTCC ACGGATAAGGAATTTGGCGGTAAGTGGACGGTAGACGGACCTGCCGACATTACTGCCGAGGTCCTTTCTC AGGCATGGGACGTTCTCCAATTAGTGAAGCATGAAGATGCGGAGGAGGAGAGAGTGACTTATGAGTCCAA ACCGACCCCGATACAGCCGTTCAATGCCTGGCCGGACGGGCCGAGTTGGAACGCGCAGGATTTTACTCGA GCGCCAATAGTTTATCCCTCTGCGGAGGTATTGGACGCAGAGGCGTTGAAAGTAGGGGCATTCGTTAGCC GAGTTTTACAATGTGTACCGTTCACGCGATCAAAGAAAAGCGTTACGGTGCGGGATGCGCAGTCGTTTTT GGGGGACTCGTTCTGGAGAATAATGCAGAACGTTTACACGGTTGTCTTACGACAGCACATAACTCGACTC AGGCACCCTTCCAGCAAAAGCATTGTTAACTGCAACGACCCTCTATGGTACGCCTACGCGAATCAATTTC ACTGGAGAGGAATGCGCGTGCCGTCGCTTAAATTAGCCTCTCCCCCGGAGGAGAATATTCAACACGGCCC AATGGCCGCCGTTTTTAGAAACGCGGGGGCTGGTCTGTTCCTGTGGCCTGCCATGCGCGCAGCCTTTGAA GAGCGCGACAAGCGACTGTTAAGAGCATGCCTGTCTTCACTCGATATCATGGACGCAGCCGTCCTCGCGT CGTTTCCATTTTACTGGCGCGGCGTCCAAGACACCTCGCGCTTCGAGCCTGCGCTGGGCTGTTTGTCAGA GTACTTTGCACTAGTGGTGTTACTGGCCGAGACGGTCTTAGCGACCATGTTCGACCACGCACTGGTATTC ATGAGGGCGCTGGCAGACGGCAATTTCGATGACTATGACGAAACTAGATATATAGACCCCGTTAAAAACG AGTACCTGAACGGAGCCGAGGGTACTCTGTTACGGGGCATAGTGGCCTCCAACACCGCTCTGGCGGTGGT TTGCGCAAACACCTATTCGACGATAAGAAAACTCCCGTCCGTGGCAACTAGCGCGTGCAATGTTGCCTAC AGGACCGAAACGCTGAAAGCGAGGCGCCCTGGCATGAGCGACATATACCGGATATTACAAAAAGAGTTTT TCTTTTACATTGCGTGGCTCCAGAGGGTTGCAACACACGCAAATTTCTGTTTAAACATTCTGAAGAGAAG CGTGGATACGGGGGCCCCGCCATTTTTGTTCAGGGCCAGCTCGGAGAAGCGGCTGCAGCAGTTAAATAAA ATGCTCTGCCCCCTTCTCGTGCCGATTCAATATGAAGACTTTTCGAAGGCCATGGGGTCTGAGCTCAAGA GGGAAAAGTTAGAGACATTCGTTAAAGCTATTTCCAGCGACAGGGACCCGAGGGGGTCCTTAAGATTTCT CATTTCGGACCATGCAAGGGAAATTATTGCAGACGGAGTACGGTTTAAGCCGGTGATAGACGAGCCGGTT CGGGCTTCAGTTGCGCTGAGTACCGCTGCCGCTGGGAAAGTGAAAGCGCGACGCTTAACCTCAGTTCGCG CGCCCGTACCGGGCGCAGGCGCCGTTTCCGCGCGCCGGAAATCGGAAATATGATAAAAATGCTTGGCATT TGCGGGCGAAGAGGCGTGATCTGAAGGGCTCCACAATGACGTAACTGAGCTACGCATCCCTATAAAGTGT ACCCGCTGACCGCTAGCCCATACAGTGTTACAGGAGGGGAGAGAGACAACTTCAGCTCGAAGTCTGAAGA GACATCATGAGCGGCTTCAGTAACATAGGATCGATTGCCACCGTTTCCCTAGTATGCTCGCTTTTGTGCG CATCTGTATTAGGGGCGCCGGTACTGGACGGGCTCGAGTCGAGCCCTTTCCCGTTCGGGGGCAAAATTAT AGCCCAGGCGTGCAACCGCACCACGATTGAGGTGACGGTCCCGTGGAGCGACTACTCTGGTCGCACCGAA GGAGTGTCAGTCGAGGTGAAATGGTTCTACGGGAATAGTAATCCCGAAAGCTTCGTGTTCGGGGTGGATA GCGAAACGGGCAGTGGACACGAGGACCTGTCTACGTGCTGGGCTCTAATCCATAATCTGAACGCGTCTGT GTGCAGGGCGTCTGACGCCGGGATACCTGATTTCGACAAGCAGTGCGAAAAAGTGCAGAGAAGACTGCGC TCCGGGGTGGAACTTGGTAGTTACGTGTCTGGCAATGGATCCCTGGTGCTGTACCCAGGGATGTACGATG CCGGCATCTACGCCTACCAGCTCTCAGTGGGTGGGAAGGGATATACCGGGTCTGTTTATCTAGACGTCGG ACCAAACCCCGGATGCCACGACCAGTATGGGTACACCTATTACAGCCTGGCCGACGAGGCGTCAGACTTA TCATCTTATGACGTAGCCTCGCCCGAACTCGACGGTCCTATGGAGGAAGATTATTCCAATTGTCTAGACA TGCCCCCGCTACGCCCATGGACAACCGTTTGTTCGCATGACGTCGAGGAGCAGGAAAACGCCACGGACGA GCTTTACCTATGGGACGAGGAATGCGCCGGTCCGCTGGACGAGTACGTCGACGAAAGGTCAGAGACGATG CCCAGGATGGTTGTCTTTTCACCGCCCTCTACGCTCCAGCAGTAGCCACCCGAGAGTGTTTTTTGTGAGC GCCCACGCAACATACCTAACTGCTTCATTTCTGATCAATTATTGCGTATTGAATAAATAA >gi|57790998|ref|YP_182402.1| tegument protein VP13/14 [Gallid herpesvirus 1] MTLPHRLTKRPFARRFCSVFVIHYSETKLDRYNKTMLLYRPDSTMRHSGGDANHRGIRPRRKSIGAFSAR EKTGKRNALTESSSSSDMLDPFSTDKEFGGKWTVDGPADITAEVLSQAWDVLQLVKHEDAEEERVTYESK PTPIQPFNAWPDGPSWNAQDFTRAPIVYPSAEVLDAEALKVGAFVSRVLQCVPFTRSKKSVTVRDAQSFL GDSFWRIMQNVYTVVLRQHITRLRHPSSKSIVNCNDPLWYAYANQFHWRGMRVPSLKLASPPEENIQHGP MAAVFRNAGAGLFLWPAMRAAFEERDKRLLRACLSSLDIMDAAVLASFPFYWRGVQDTSRFEPALGCLSE YFALVVLLAETVLATMFDHALVFMRALADGNFDDYDETRYIDPVKNEYLNGAEGTLLRGIVASNTALAVV CANTYSTIRKLPSVATSACNVAYRTETLKARRPGMSDIYRILQKEFFFYIAWLQRVATHANFCLNILKRS VDTGAPPFLFRASSEKRLQQLNKMLCPLLVPIQYEDFSKAMGSELKREKLETFVKAISSDRDPRGSLRFL ISDHAREIIADGVRFKPVIDEPVRASVALSTAAAGKVKARRLTSVRAPVPGAGAVSARRKSEI Virmugen A UL47 mutant in avian infectious laryngotracheitis virus is attenuated and provides protection in chicken against subsequent challenge infection with virulent ILTV [Ref1974:Helferich et al., 2007]. Chen et al., 2011 journal Chen HY, Zhang HY, Li XS, Cui BA, Wang SJ, Geng JW, Li K 2011 60 Pt 1 110-116 Journal of medical microbiology Fuchs et al., 2007 journal Fuchs W, Veits J, Helferich D, Granzow H, Teifke JP, Mettenleiter TC 2007 38 2 261-279 Veterinary research Han et al., 2002 journal Han MG, Kweon CH, Mo IP, Kim SJ 2002 147 5 1017-1031 Archives of virology Helferich et al., 2007 journal Helferich D, Veits J, Teifke JP, Mettenleiter TC, Fuchs W 2007 88 Pt 3 732-742 The Journal of general virology Veits et al., 2003 journal Veits J, Lüschow D, Kindermann K, Werner O, Teifke JP, Mettenleiter TC, Fuchs W 2003 84 Pt 12 3343-3352 The Journal of general virology