Chlamydia muridarum 83560 C. muridarum naturally infects only members of the family Muridae (which includes both mice and hamsters) (Wiki: Chlamydia muridarum). Chlamydia muridarum is an intracellular bacterial species that at one time belonged to Chlamydia trachomatis. However, C. trachomatis naturally only infects humans and C. muridarum naturally infects only members of the family Muridae (includes both mice and hamsters, Alderton, 1996). Two strains of Chlamydia muridarum, MoPn (originally named Nigg) and SFPD, have been isolated from mice and hamsters. Glycogen production by both strains has been demonstrated, although it is difficult to detect glycogen in MoPn (Moulder et al., 1984). Chlamydia muridarum MoPn binds mAbs recognizing Chlamydia trachomatis MOMP vs4 core epitope (T)LNPT(IA). DNA sequence analysis indicates that these mAbs should recognize SFPD and that Chlamydia trachomatis B-serogroup mAbs specific for the vs4 epitope IAGAG should recognize SFPD (Wiki: Chlamydia muridarum). Baboon Papio cynocephalus 9556 Bank vole Clethrionomys glareolus 447135 Bear Ursus americanus 9643 Birds Passeroidea 175121 Brown Trout Salmo trutta 8032 Buffalo Bison bison 9901 Carnivores Vulpes 9625 Cat Felis catus 9685 Catfishes Siluriformes 7995 Cattle Bos taurus 9913 Chicken Gallus gallus 9031 Chimpanzee Pan troglodytes 9598 chinchillas Chinchillidae 10150 Copper Pheasant Syrmaticus soemmerringii 9067 Deer Cervus elaphus 9860 Deer mouse Peromyscus maniculatus 10042 Dog Canis familiaris 9615 Ducks Anas 8835 Ferret Mustela putorius furo 9669 Fish Hyperotreti 117565 Gerbil Gerbillina 10045 Goat Capra hircus 9925 Gray wolf Canis lupus 9612 Guinea pig Cavia porcellus 10141 Hamster Mesocricetus auratus 10036 Horse Equus caballus 9796 Human Homo sapiens 9606 Macaque Macaca fascicularis 9541 Mongolian Gerbil Meriones unguiculatus 10047 Monkey Platyrrhini 9479 Mouse Mus musculus 10090 None None Parrot Psittacidae 9224 Pig Sus scrofa 9823 Rabbit Oryctolagus cuniculus 9986 Rainbow trout Oncorhynchus mykiss 8022 Rat Rattus 10114 Raven Corvus corax 56781 sei whale Balaenoptera borealis 9768 Sheep Ovis aries 9940 Squirrel Spermophilus richardsonii 37591 Tree shrew Tupaiidae 9393 Trouts, salmons & chars Salmoninae 504568 Turkey Meleagris gallopavo 9103 Vole Microtus ochrogaster 79684 Water buffalo Bubalus bubalis 391902 C. muridarum DNA vaccine encoding TC0439 VO_0011533 DNA vaccine Research pCI30 Subcutaneous Subcutaneous C. muridarum TC0439 adherence factor DNA vaccine construction The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007). BALB/c Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007). The groups of female BALB/c mice immunized intra-abdominally with TC0439 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007). All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007). C. muridarum DNA vaccine encoding TC0512 VO_0011534 DNA vaccine Research pCI30 [Ref1057:McNeilly et al., 2007] Subcutaneous injection Subcutaneous injection C. muridarum TC0512 outer membrane protein, putative DNA vaccine construction The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007). BALB/c Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007). The groups of female BALB/c mice immunized intra-abdominally with TC0512 showed significant levels of protection when compared to negative control animals. At 9 days following challenge TC0512 showed a 73% reduction in the number of recoverable Chlamydia compared with vector only immunized controls (McNeilly et al., 2007). All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007). C. muridarum DNA vaccine encoding TC0757 VO_0011522 DNA vaccine Research pCI30 Subcutaneous injection Subcutaneous injection C. muridarum TC0757 DNA vaccine construction The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007). BALB/c Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007). The groups of female BALB/c mice immunized intra-abdominally with TC0757 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007). All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007). C. muridarum DNA vaccine encoding TC0767/768 VO_0011524 DNA vaccine Research pCI30 intra-abdominal injection intra-abdominal injection C. muridarum TC0767/768 DNA vaccine construction The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007). DNA vaccine construction The C. muridarum genomic expression library was created by ligating fragments of C. muridarum DNA into the novel expression vector, pCI30. Eighteen recombinant C. muridarum clones were selected for immunizations and these were grown on LB agar. Cultures were grown for 18 h at 37 °C with shaking at 225 rpm. Following this, 1 mL of the starter culture was used to inoculate 80 mL of selective LB broth and the cultures again incubated for 18 h at 37 °C with vigorous shaking. DNA was then extracted from cell pellets using Roche Genopure Plasmid Midi kits (Roche Diagnostics Australia Pty Ltd.) (McNeilly et al., 2007). BALB/c Mice were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia) and the abdominal fur of each mouse was removed with electric clippers. The barrel liner of the Helios gene gun was held directly against the abdominal skin and a DNA/microcarrier shot delivered using a helium pressure of 400 psi. Each animal received two shots with the appropriate DNA/microcarrier preparation, resulting in administration of approximately 2 μg of DNA. Each mouse was immunized three times at 3-week intervals. Positive control mice received 2.5 mg of medroxyprogesterone acetate (Ralovera) (Kenral Division of Pharmacia Australia Pty Limited, Rydalmere, Australia) subcutaneously 7 days prior to receiving a dose of 5 × 10^4 live C. muridarum elementary bodies (McNeilly et al., 2007). The groups of female BALB/c mice immunized intra-abdominally with TC0767/768 showed significant levels of protection when compared to negative control animals (McNeilly et al., 2007). All animals were challenged intra-vaginally with 5 × 10^4 live C. muridarum elementary bodies, 10 days following the final gene gun immunization, as described for the positive control mice. To assess the level of infection, cervico-vaginal swabs were collected from each animal every 3 days following the intra-vaginal challenge for a period of 3 weeks (McNeilly et al., 2007). C. muridarum MOMP protein vaccine VO_0011453 Subunit vaccine Research Intranasal immunization Cholera toxin and CpG oligodeoxynucleotide Intranasal immunization C. muridarum major outer membrane protein MOMP Recombinant protein preparation Recombinant maltose binding protein-MOMP (MBP-MOMP) was cloned into the bacterial expression vector pMAL-c2, and used to transform Escherichia coli (DH5α[pMMM3]). The MBP-MOMP was purified by passage through a PD-10 column (Amersham Biosciences, NSW, Australia), followed by a Sephadex G-75 column (Sigma–Aldrich) (Skelding et al., 2006). BALB/c Groups of five mice were immunized on days 0, 7, 14, and 35 via either the IN or TCI route. One group of five mice were used as a control, receiving no immunization solution. Mice immunized intranasally were anaesthetised via inhalation of 4% isofluorane (Abbott Australasia, NSW, Australia), and 10 μl of immunization solution containing 100 μg MOMP, admixed with 10 μg CpG-ODN 1826 (5′-TCC ATG ACG TTC CTG ACG TT-3′) (Geneworks, SA, Australia), and 10 μg cholera toxin (Sigma–Aldrich) was gently pipetted onto each nare (5 μl per nare) (Skelding et al., 2006). Both intranasal and transcutaneous immunization protected mice against respiratory challenge with Chlamydia (Skelding et al., 2006). Five days after the final boost, mice were infected intranasally with C. muridarum. Mice were anaesthetised with 4% isoflurane and 10^3 IFUs were gently pipetted onto the nares. Mice were weighed daily for 10 days then sacrificed 10 days post-infection via a sodium pentobarbital overdose (Nembutal, Abbott Australasia). The lungs from the mice were either perfused and fixed for histopathology or collected for determination of C. muridarum levels (Skelding et al., 2006). Both TCI and IN immunization resulted in greatly increased IFN-γ gene expression in stimulated lymph node cells (stimulated with MOMP) as compared to non-immunized mice (Skelding et al., 2006). Both routes of immunization induced a significant MOMP-specific IgA response in serum with the highest IgA response seen in animals immunized intranasally. These results were compared 7 days post-immunization to non-immunized control mice (Skelding et al., 2006). Both routes of immunization (TCI and IN) resulted in levels of MOMP-specific IgG in serum that were significantly higher than that in serum of non-immunized control mice. There was no significant difference between serum IgG levels in animals immunized by either route. These results were gathered 7 days post-immunization (Skelding et al., 2006). C. muridarum PmpE/F-2 protein vaccine VO_0011456 Subunit vaccine Research Intravenous injection (i.v.) Incomplete Freunds Adjuvant (Sigma) Intravenous injection (i.v.) C. muridarum polymorphic membrane protein E/F family protein Recombinant protein preparation The source proteins containing the MHC II binding Chlamydia peptides were cloned, expressed and purified as follows: pmpE/F-2 and gap DNA fragments were generated by PCR using genomic DNA isolated from C. muridarum. The PCR products were purified and cloned into pET32a (Novagen) for pmpE/F-2 and gap after restriction enzyme digestion with BamHI/NotI using standard molecular biology techniques. Only the first half of the gene (25–575) was cloned into the vector for expression (Yu et al., 2009). C57BL/6 Mice were vaccinated three times with a 2-week interval, intravenously (i.v.) into the tail veins with 1 × 10^6 DCs transfected with Chlamydia protein PmpE/F-225–575 in 200 μl of PBS. DCs pulsed with live EB or GST protein was used as positive or negative controls respectively (Yu et al., 2009). PmpE/F-2(25-575) immunized mice exhibited significant resistance to challenge infection. In addition, pmpE/F-2 was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009). Two weeks after the final immunization, five to ten mice from each group were intranasally (i.n.) challenged with 2000 IFU of C. muridarum. Weight loss was monitored each or every two days. On 10 day after i.n. challenge, the mice were euthanized and the lungs were collected for Chlamydia titration (Yu et al., 2009). C. muridarum PmpG-1 protein vaccine VO_0011458 Subunit vaccine Research Intravenous injection (i.v.) Incomplete Freunds Adjuvant (Sigma) Intravenous injection (i.v.) C. muridarum polymorphic membrane protein G family protein Recombinant protein preparation The source proteins containing the MHC II binding Chlamydia peptides were cloned, expressed and purified as follows: pmpG-1 and gap DNA fragments were generated by PCR using genomic DNA isolated from C. muridarum. The PCR products were purified and cloned into pET32a (Novagen) for pmpG-1 and gap after restriction enzyme digestion with BamHI/NotI using standard molecular biology techniques. Only the first half of the gene (25–500) was cloned into the vector for expression (Yu et al., 2009). C57BL/6 Mice were vaccinated three times with a 2-week interval, intravenously (i.v.) into the tail veins with 1 × 10^6 DCs transfected with Chlamydia protein PmpG-1 25–500 in 200 μl of PBS. DCs pulsed with live EB or GST protein was used as positive or negative controls respectively (Yu et al., 2009). Overall, vaccination with DCs transfected with PmpG-1(25-500) exhibited the greatest degree of protective immunity among the four Chlamydia Ags tested (Yu et al., 2009). Two weeks after the final immunization, five to ten mice from each group were intranasally (i.n.) challenged with 2000 IFU of C. muridarum. Weight loss was monitored each or every two days. On 10 day after i.n. challenge, the mice were euthanized and the lungs were collected for Chlamydia titration (Yu et al., 2009). C. muridarum PmpG-1, PmpE/F-2, and MOMP Proteins Vaccine VO_0004257 Subunit vaccine Research subcutaneous injection subcutaneous injection Chlamydia proteins PmpG-1, PmpE/F-2, and MOMP (Yu et al., 2010). C57BL/6 and BALB/c Mice were vaccinated 3 times subcutaneously with PmpG-1 plus PmpE/F-2 plus MOMP plus AbISCO-100 (G+F+M+AbISCO) (Yu et al., 2010). The combination of three Chlamydia proteins formulated with AbISCO exhibited significant protection at day 13 compared to the group with adjuvant alone (Yu et al., 2010). Two weeks after the final immunization, the mice in the three animal trials described above were then challenged with live EBs for protection evaluation (Yu et al., 2010). C. muridarum RplF protein vaccine VO_0011437 Subunit vaccine Research Intravenous injection (i.v.) Incomplete Freunds Adjuvant (Sigma) Intravenous injection (i.v.) C. muridarum rplF Recombinant protein preparation The source proteins containing the MHC II binding Chlamydia peptides were cloned, expressed and purified as follows: rplF DNA fragments were generated by PCR using genomic DNA isolated from C. muridarum. The PCR products were purified and cloned into pGEX-6P-3 (GE Healthcare) after restriction enzyme digestion with BamHI/NotI using standard molecular biology techniques (Yu et al., 2009). C57BL/6 Mice were vaccinated three times with a 2-week interval, intravenously (i.v.) into the tail veins with 1 × 10^6 DCs transfected with Chlamydia protein RplF in 200 μl of PBS. DCs pulsed with live EB or GST protein was used as positive or negative controls respectively (Yu et al., 2009). RplF was able to engender protective immunity against challenge with C. muridarum (Yu et al., 2009). Two weeks after the final immunization, five to ten mice from each group were intranasally (i.n.) challenged with 2000 IFU of C. muridarum. Weight loss was monitored each or every two days. On 10 day after i.n. challenge, the mice were euthanized and the lungs were collected for Chlamydia titration (Yu et al., 2009). C. muridarum Tarp subunit vaccine VO_0004202 Subunit vaccine Research Intramuscular injection (i.m.) CpG-IFA adjuvant Intramuscular injection (i.m.) purified C. muridarum Tarp Recombinant protein preparation BALB/c Mice were immunized intramuscularly with purified C. muridarum Tarp emulsified in CPG-IFA adjuvant or just adjuvant (as a control). Mice were immunized in the hind legs 3 times with an interval of 3 weeks between the first and second injections and 9 days between the second and third injections (Wang et al., 2009). Immunization of mice with Tarp induced Th1-dominant immunity that significantly reduced the shedding of live organisms from the lower genital tract and attenuated inflammatory pathologies in the fallopian tube tissues. Tarp, an immunodominant antigen identified by human antisera, can induce protective immunity against chlamydial infection and pathology in mice (Wang et al., 2009). Thirty days after the third immunization, each mouse was inoculated intravaginally with 1 × 10^4 IFUs of live C. muridarum organisms in 20μl of SPG (sucrose-phosphate-glutamate buffer). Five days prior to infection, each mouse was injected with 2.5mg Depo-provera subcutaneously to synchronize menstrual cycle and increase mouse susceptibility to chlamydial infection (Wang et al., 2009). Ifng (Interferon gamma) Mouse 15978 33468859 NM_008337 NP_032363.1 MGI:107656; UniProt:P01580 10090 ? >gi|145966741|ref|NM_008337.3| Mus musculus interferon gamma (Ifng), mRNA ATAGCTGCCATCGGCTGACCTAGAGAAGACACATCAGCTGATCCTTTGGACCCTCTGACTTGAGACAGAA GTTCTGGGCTTCTCCTCCTGCGGCCTAGCTCTGAGACAATGAACGCTACACACTGCATCTTGGCTTTGCA GCTCTTCCTCATGGCTGTTTCTGGCTGTTACTGCCACGGCACAGTCATTGAAAGCCTAGAAAGTCTGAAT AACTATTTTAACTCAAGTGGCATAGATGTGGAAGAAAAGAGTCTCTTCTTGGATATCTGGAGGAACTGGC AAAAGGATGGTGACATGAAAATCCTGCAGAGCCAGATTATCTCTTTCTACCTCAGACTCTTTGAAGTCTT GAAAGACAATCAGGCCATCAGCAACAACATAAGCGTCATTGAATCACACCTGATTACTACCTTCTTCAGC AACAGCAAGGCGAAAAAGGATGCATTCATGAGTATTGCCAAGTTTGAGGTCAACAACCCACAGGTCCAGC GCCAAGCATTCAATGAGCTCATCCGAGTGGTCCACCAGCTGTTGCCGGAATCCAGCCTCAGGAAGCGGAA AAGGAGTCGCTGCTGATTCGGGGTGGGGAAGAGATTGTCCCAATAAGAATAATTCTGCCAGCACTATTTG AATTTTTAAATCTAAACCTATTTATTAATATTTAAAACTATTTATATGGAGAATCTATTTTAGATGCATC AACCAAAGAAGTATTTATAGTAACAACTTATATGTGATAAGAGTGAATTCCTATTAATATATGTGTTATT TATAATTTCTGTCTCCTCAACTATTTCTCTTTGACCAATTAATTATTCTTTCTGACTAATTAGCCAAGAC TGTGATTGCGGGGTTGTATCTGGGGGTGGGGGACAGCCAAGCGGCTGACTGAACTCAGATTGTAGCTTGT ACCTTTACTTCACTGACCAATAAGAAACATTCAGAGCTGCAGTGACCCCGGGAGGTGCTGCTGATGGGAG GAGATGTCTACACTCCGGGCCAGCGCTTTAACAGCAGGCCAGACAGCACTCGAATGTGTCAGGTAGTAAC AGGCTGTCCCTGAAAGAAAGCAGTGTCTCAAGAGACTTGACACCTGGTGCTTCCCTATACAGCTGAAAAC TGTGACTACACCCGAATGACAAATAACTCGCTCATTTATAGTTTATCACTGTCTAATTGCATATGAATAA AGTATACCTTTGCAACC >gi|33468859|ref|NP_032363.1| interferon gamma [Mus musculus] MNATHCILALQLFLMAVSGCYCHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQI ISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQ LLPESSLRKRKRSRC IFN-gamma plays a critical role in Th1 type immune response. It is important for protection against infections by various viruses and intracellular bacteria. Vaximmutor The experimental data demonstrated that three time vaccinations with BCG in BALB/c mice induced strong TB Ag-specific IFN-gamma immune responses in splenocytes [Ref2101:Wang et al., 2009]. IgA Mus musculus 238447 AC160982 10090 12 8607 12639 - immunoglobulin heavy constant alpha Also known as IgA; Igh-2 >gi|121699722:8607-12639 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12 AGTACTGGGGGACCTCTTTGCTGCCAAACGGGCCTCGAACAGTTGTAACAGTGAGTGCTGTGCTGTAGAA CAAGCTCAGTAGGAAGAGGGTGAGGAAGGTCACAGTGGTGGGCCACAGGCTGGCACCTGGGGCCTCTTCC TCCAGGATGTCTTCTGACTGGTCCAGTAGCACATAGGAAAGTGGCTCTTGACGTTCTGTAAGACATAGAT GACCTTCTAGATCCAAACCCATGGGGATGGGGGCTCAGACTTAAGGTTTCTGCTTCTATGCCTCCTTGGG CTTTGGGGACAGTGGTGGTAGGGGTCCCTTATTCCCGGGAAGGGACTTGTTCAGGGATAATGAGGAGGCA GGGTCCCAGGTAAGGGACTGGAATCCCAGCAGTTCTTGAGATGGCCCCAAGACCCATCTCTCTCTCTTAG GGAAACCTTAATTAGAGACCTGTCATGGTAAGAGAGTTGTTTCTGTCAAACTCTGAGAAGAGTTTGAACC CAAGGCTCCTTAGGTCTCAGTCCTTTCTAGATATTTAGCAATACCTCCCTAAACCCCAGTTCTTAGGCTA ATCTTGGCTTGGGTACTAGGGTTGTGTAAGATCAGACACCTTCAAACAGATACAGGCCAATGTGATTTCT GGGCCAAAGTTAGACTACCAAGGCCTGGACATGTGGTCAGAGGACTCTGTTGCATTCTTAAGAGCTGTGA CTGGGGGTTAAGCAACAGTAAAGACCCCCAGTAGAGCCAACCCTGCTGTCCTCAGGTTCTCTGCGCACAG AGTTTCATTTTAAGTGGCAGTAGGAAATGTGTACTGATAGTCAACCACTCGTATTTCTGTCACTGCAGCC ATCCATCAATCCATCCATCATCCCCATCGTTCATCCTTGTAGCCAGCCATCCATTACCCTCCAGCCGCTC ACTCATCATCTTTCCCGACTATCGTGATTCTCCAGCTTCTCTGCCCCCTCTCCCTGTGGGGATTCAGTCT TTCACCTATTGACTCCTCCTTACTAGGATTCTCTGTCATGGGCCAGGCCTTGGATTCCATGGGTGGCTAT GGTACTTTTCTGAGTCAGGTTTCCCAAGCTGGTCTGTTGCTCTTGCTATTCTCAGGGAACTGGACTTTCA AGCTGGAGAGTATCCAGGGCATGCATGGGCCTTTACTCCACTCTTGCTCTGAGAGCAGAGGGAAGTTGCT AGAAACCAGCAACTCCTCCCCCCCCCCCCCGAAGTTCTGAAGGCCTGTGAGACAGGAGCAGGGCCCCCAC TGCCCTCTTGTGGACACCTGGGCTCCAAGTATACCTGGTTGTAGAACTTTGTGTTCTAGGGGTGCCCATG GAGGAGGCCGAGGCCTGTGTGGGAGTGGTCTCCATGGCCTGCTCCTGCTTCTTCCCAGATTTGCCTCTTA TCCTGCTTCTGGTTTGTGATACTTGGGCTCAGGATGGGAAGGGAGACTTAGGTTAGAAAGGGGGCACCTG GAGGGGCCTATGCCCTTGTCCATTGTGTGTAGTTGTCAGTGTCGGGCCTGGAAATTCTCCCGGGGGCTTG GAGGGGACTCCCCTTGCTTTCCAGAAGCTGGGTGTGGGCTGTGAACTTCATCTTCCTCCAGGGTTCAGGT CTGTGTGAGTGTGCAAATGTGAGTGTCTGTGCATGTATGCCCATGTGCATGTATGCAGAACAGGGTGTAT GCCCATGTGCATGTGTGAAGAGCAGGGTGCTGCTGAGGGTTGTCTGTGCCATGCTATATGTCTTTCTCAT GAGTCAGTGGTCACCCTGAATGCATGTTTCCATTCAGGGATGGCCTACTGAGGGTGCGTGGCATCTTCTT CCCAGTGCCCCTGTGTTCATCCCTTCATTCTCATACCATCATCTCTATTGTGACCCCCACACGGCACCAG CCTTGATGGTGCTACCCTTTTGCTCCATCTATCCCTGTCTTGTTTGGGTTAGAAGCTGCGGTCTAATAAA CAGCCACACCACCCACCACTCAGAGTACTTGTGCAGCAACCCACTGCCCTGGGGACTCTCCCATGAGCTT TGCCTTCTTGGCTGAGTCTGCTCGCCTTGGATTTCATCCCCAGTTTATTCTTACTTCCCATTCACTCATT GCACATTCACAGGGCAGGTGCACACACTCTCATGTTTCCACCAACATGAACACACATAGATATATGCATG CTCATAGGTATGCACAGGCACACACTTGCTCACAGGGACATAGATATAGTGTGTCCACATATGTACTTAT ACCCATATACATACCTATAAACCATAGGTGCACACAAACTCACTCCTCTCTTGCTGCACACAGGTACACA CATGAAGGCACATGGACCCATCCATAAGCACTCACCAATACATAGACATGCATACACATATACTCTAGGC ATGAAGGGTCTTCAAAGTCTCCGAGGCCTTACAAGCTTAGGTGGTGGTGTTTCTCCCTCCCTGGGCCCTG ACCCCTCCCTGTGTTCTGTAGACCCTAAGGATGATGGACAGGCACTGGATGGAAGTGCAGGGATACTTTG GATGAGCACAGAGTTTATTTCAGGAGTAGGGACAGGCAGGGTGGCTCAGTAGCAGATGCCATCTCCCTCT GACATGATCACAGACACGCTGACATTGGTGGGTTTACCCGACAGACGGTCGATGGTCTTCTGGGTGAAGT TCATGGGCAAGGCCTCGTGGCCCACCATGCAGGAGTACTGGTCACCCTGTTTCCAGAGTTCAGCTGATAC ACGCAACACGCTTGTCACCAGGTAGGTGGTGGCTCCCTCGCCTGGCTCCTTTAGGGGCTCAAACACTAGG TAGCTTTCTGGGGACAGCTCCTCATTTCCATGCAGCCATCGCACCAGCACTTCTTTAGGGTTGAAAGCTC GCACCAGGCATGTCAGGGACACGAGCTCATTCAGGGCCAGCTCCTCCGACGGCGGCGGTAGCAGGTGGAC CTGGGGTGGGAAGGTGTTCACTGGAAGGTAGAAAGAAGAGTTATGGCTCAGACAAGGAGAGCAAGACCCC TCTGCCCTTCCCTTCTGGGCAGTTCCTCAGATAGCTCCAATTTCCCTCTGTGATATAAGGACAGGAGCAG TTAGTTGAGCATCTGTATTATAGGAAGAAAGCAGGCATGTGAGCAGGGAACGTCATACAATGTCCCGGGT ATGCATCTGGGCTCACCTGTGATTTTGGCAATTGTGCCAGTTAAGGTGTCAGACTCAGGATGGGTAACTG TGCACTTGAATGATGCGCCACTGTTCCAGCGCTCAGCACAGCCAGGCAGGACGCTGGACACACTGTAGCA GCCGCAGGAATTCTGCACAGCTTTCTTCTGCACTGCATCCTTCCCAGTGGAGGGCTCCCAGGTGAAGACA GCTCCCTCAGGATTTCTCAGGCCATTCAGAGTACATGTGAGGCTGGCATCTGAACCCAGGAGCAGGTCCT CAAGAGCTGGCCGCTGCAGTGACAGGCTGGGATGGCAGGAAGGAGGACAAGGAGGACAAGGAGGAGGAGG ACCTGTAACAGAGAAGTCCTAGCAAATCAGTATATTCTCCTTTTCTCCTTTGCTGTCCCCTTTTCTGGAT GTTCTGAGGCCTCAGTTCCTTATGGCCTGTTTACGCTTCCCTTCCCCCACCCCCATGGGAGGTTTCTGAG GGTCCCTGTGAGGGTGTGCCTCAGAGGGATATATGGATCTAGATAAGGTAGAACTTATCCCACCCCCAGC TGACCCCCTAACGTTCTTTACCAGAGCACTTCACATCCAATTCTTGGACGGCGTTAGAGTCATGTTGCAC GGAACATTTCACGGATTCTCCTTCTGGGCACTCGACAGCTGGCAGGGTCAACTGGCTGCTCATGGTGTAC CCTCCCCCAGAGGCCAGGGCAGGTGGGAAGTTTACGGTGGTTATATCCTTCCCACTCTTTCCCCAGGTCA CATTCATCGTGCCGGAAGGGAAGTAATCGTGAATCAGGCAGCCGATTATCACTGGGTCACTTGACAGAGC TCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGAC Vaximmutor IgG Mus musculus 16059 AF010213 10090 12 881411 914690 + immunoglobulin heavy chain (V7183 family) Also known as IgG; IgH; VI24H; VH7183; B9-scFv; IgVH1(VSG) >gi|94393741:881411-914690 Mus musculus strain 129/SvJ chromosome 12 unlocalized genomic contig, MGSCv37 alternate locus group 129/SvJ CATGGACTTTGGGCTCAGCTGTGTTTTCCTTGTCCTCATTTTAAGAGGTAATTTGTAGAAATAAGATCCT GCCAGTATTGTGTACAGGAGAAATAGAAAAATTTTTCTTTCCTCTATTTTGTTTTGTTTTGTTAGTGACA GTTTACAAATAAGCATTCTCTGTTGTGAGGTGCCCAGTGTGAGGTGAAGATGGTGGAGTCTGTGGGAGGC TTAGTGCAGCCTGTAGGGTCATTGAAACCCTCCTGTGCAGCCTCTGGATTCATTCTCACTGACTACTGAA TGACCTGGATCCTTCAGGCTTCAAAGAAAAGGATGGAGAGGGTGACAATAAAATTTTTCCCACTCACACC CACTTGCTGGCCCTGGCGTTCCCCTGTAATGAGGCACATAAAGTTTGCAAAACCAAGGGGCCTCTCTTCT CAATGATGGCTGACTAGGACATCTTCTGATACATATGCAGCTAGAGACATGAGCTCCCGGGATACTGGTT ACTTCATATTGTTGTTCCCCCACAACAGTTGCAGACCCTTTTATTTCTGTGGGTACTTTCACTAGCTCCT CCATTGGGGGCCCTGTGTTCCATCCAATAGCTGACTGTGAGCATCCACTTCTGTAGTTGCTAGGCCCCGG CATAGCCTCACAAGAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTTGCTGGTGTATGCAATGGTGTC AGAGTTTGGAGTCTGATTATGGGATGGATCCCCAGGTATGGCAAACTCTAGATGGTCCATCCTTTCCCAA CAATAATTTTTAATGGTGGAGATAGCACCTACTATCCAGACACAATGAAGGGCCAATTCACTATCTCTAG AGATGATGCCAAAAACACACTTTACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTATACA TATATTCCTAAGCTTGAGTAAGTGGACTTAAACTGATTCCATCACAACTTGCATGAGATGGATATTCCCC AGTGTTAAGACCTGTCACCATCACTGTCAATCAGAAGACAAAGTTTATGCACAACAAAACAAAAAAACCA AAAGCAGAGGCCTCCAATTACAAGTAATAGACCCAGACCCACAGTCTCTGAAAACTGACTGTACAGTTGG ATCCAGTCTTTTACTTCTTTTCCCTGGATTTTATATTACTGAGGAAATGAGGAAAGCTCTACAATATCTG TTCTCCATAGTGCTCAACACCTCCAAGCACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTTCTT GTCTTGTAGACTACTTCAACCTATTTTGTACTCCAGTTAATGAAACTCAAGTCTAGCAGCCTGTCACTGT TTATTCTAAAGTATTATGAACAGGTGACCTCCCATCCTTCCCCAACGCAATAATCATATTTAGGAATTTG AGGTTTTATGAGATATGATCTCAGGGTAGAGAGAGAAAGCAAACTACATAGAAATATAGACTGACATAAA TCAAGACTTGCATAAGCTAGTCCCCAAGTTCCATGTCCCTAAGTGGCAAGGACTATCTTCTGAGCCTAAT GAGATGAGATCCAAATCAAACCTCCTGGGTCTTTTTAGATAAACATGTGAGATCAATAGACTAAATGCTT TGGCTGGGCTTCCTTGCAATCCAATTCCCAAACAAAAATGGATCTGGCTCCACAGACACCACAAGAATAG TCTTAAATAGTTCTTTAAGTAGAATGTCTGATCACTACGAGCCCAATTCCATCCTAAATACTCTTCTGGA TTATACATAAATAAAAATTGAACATAGGGCATGGGGCACTGATCTCCCTGTGCTACATGAATGGGGGCTC ATTTACTAAATGTTCCCATTTTTCTTTCTAGCGCTGCACAGTGCAAATCCTACAACTTCCTGTTTATCTA CAATGTGAACTCCAAACAGTACAAGAAAAAACGTTCCTTATGTTCCTCTCCAGGTTCTCCAACAAGCACA GAGCAACCTTCTGTCACCAGGACGGAAAACTGGAAACCTTGCTCACTGCTTCCTTTTATTCCCTCGGGAA CCTCCCCCAATGCAAAGCAGCCCTCAGGCAGAGGATAAAAGCTCACACAAAGACGAGAAGCCCCATCCTC TTCTCATAGAGCATCCATCAGAGCATGGCTGTCCTGGGGCTGCTTCTCTGCCTGGTGACTTTCCCAAGCT GTAAGTGTTTCAGGGTTTCAGAAGAGGGACTAAGACATGTCAGCTAGAAGATGTGTGACTAATGGTGATG TTGCTTGTCCCCAGGTGTCCTGTCCCAGGTGCAGCTGAAGGAGTCAGGACCTGGCCTGGTGGCGCCCTCA CAGAGCCTGTCCATCACATGCACCGTCTCAGGGTTCTCATTAACTAGCTATGGTGTACACTGGGTTCGCC AGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTGATATGGAGTGATGGAAGCACAACCTATAATTCAGC TCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTC CAAACTGATGACACAGCCATGTACTACTGTGCCAGAAACACAGTGTGGGAAGTCCAATGTGAGCCTGCAC AAATACTTCTCTGCAGGGATGATCACAACCAGCAGGGGGCGCTGATGACCCAAAGGGACTTCCCAGGATC TCTTCTGGAATCTAGGGAGTTCTGGCCTGTGTCTATCAGCATGTGTTTCAATGTTAGAGTTGTGAGTTTT CCTTCCAGCAACAGAGATATTTTAGAGCCCACTTTTCATGGTCATTCTACTAAATTTGTTCACATAGTGG AAAGATTTGTTAAATGATTCTATAGTCTAATAGGGTCAAACAAAAACAAATAATTGAGTTCATATCTACC AAAAAAAAAAAAATTCCCTCAAAGTGGACAACTGTGTAGGTGAGGAAAACCAGGGGGATTTGGGAATAGA TTATTTCTCTCCCGACTGTGGTGTTAGTCACCTTTCAGCCATTTACTGTATTTAATTCATGTTTAAAGTT AATTGTATTCATCTTCCCTAACATGAAGTTTTCAAACACATGTCCACAATTTAATGATGACACAGTTATC ATTGTGAATGATAACTCATAAGATCTCTCTCCATATATGAAACACACGATATTCTGTTATTAGCTATAGC CAACAAAGTTACATATCATTATTATTACTGAAATAATTCTTCCCATCTAACTGAATAGTTTTCTCACTCG ACACTGCCTATGCTGCGATCAGCCTGCTCTAATTTTACCTTTGCTCAGAGCACTGCTTTCTGTTTCATTT GATAAGATGGTGCCTGGTTGTCACTGAAAATGTGTCCTCCAGTTTCACAGTGATTAATGTGATTTTCAGT ACATTGAGAACAGAGCCACAGCAAAAATGAGATGGAATGTCATCATTTTTACGCCAGGTGCTGCAGAAAT AGCTAGGTGGTTAAGAGAGAATATTGAACTTTGAGGGGTTCAGATTAAATTCCTGATACCCACAATGTGC ACCTCATAACCACATGTACATATAGAAAACTCAGTATAAATGTGGCCTCCGTGAGCACCACACTACTCCC ACAAACACATATACACATAATTAAAAGTAAAGATTTTAGAAAAATGGCTAATCTAATGATAGGAAGTAGT CAAAAAGAGAGTTCTTTTGTCATGTACATATGTGTAGCAGACTTAAATGTTAAACATTCAAGAATACATT CCTCGATCCACATTAAAATTTTGCAAAGAGTAGCACAGACGGTGGCAATTGCTAAACTTATATCTAGAAA CACAATTATGTGTGGTGATATTTAATTACACATTTATACCAGGACATATGACAATATGGAAACCAAACAT GTTGTATCCACATGCTCTAAGGAAAACTAAATGGAGTGTGATAAAACCAAAGAAAATGTGAATATGAAAT ATTTTTCCAACTCTGCATCTTAAAACGGTTTCTTTCATGTGTCATATCTGCTATGAGGACTTTCTTCTGC CCATGTCCAACTCCAGAGCATGCCACAGCAGGAAGACCTACAGGTATTACTTCTCTGCACCCAGGAAAAC CACCTCTGTCCTGACCCTGCAGCTCTCAGAAGAGCCCAGACCTTCATTCTCAGGCCCTCATCCAGTAATC AGCACTGAATACAGAGCACTCACCATGGACTTTGGGCTCAGCTTGGTTTTCCTTGTCCTTATTTTAAAAG GTAATTCATAGAGATAAGATTCTATCTGTTTTGTGTACATGAGAAACAGAAAAATTGTATTGTTTCTCTA TTTTGTTTTGTTTTGTTAGTGACAGTTTCTGACTCAAGATTCTCTGTTTGAAGGTACCCAGTGTGAGGTG AAGCTGGTGAAGTCTAAGGGGAGGCATAGTGCAGCCTAGAAGGTCCATGATACTCTACTGTGCAGCCTCG GATTCACTGTAAGTGACGACTGGTTTGTCCGTGTTTGCCAGGCTCCAAAGAAGGGGCTGCAGTGGGGGAT GGGAATAATTTTTCATGGTTGTGGTAGCCCCTCTTATGCAGACACCTTGAAGAAGTGGGTTGGACGTAAC ATATTCAGAATCAATATTTAAAGATTCTAATCCTTGAAGATATCACTTTTGACCAAGTATATATGAACCA TGTTACTGAGGTTTATGGAGGTTTGAGTATGTTAGGTCCATGGATAGGGAAAATATTAGGGGATTTTAGG AGTAACTGAGGCTTGTTGTAGGAAGGACATCACTGTAGGGGTAGGCTCCGTGTTCCTATCTTCAAGCTCT ACCCAGTGCAGAATAGAGCCCTCTTCTGCCGGCAAGTGGAAAGAGTTTCTCTTCCTGGCTGCCTTCAATC CAAGTTGTAGAATATCAAATCTCCTAACACTATGACTGCGAGCATGCTGACATGCGTCCCACCATAAAAA TAGACTGAACCTCTGAAGCTTTAAGCCAGCCTCTAGTAACTGTATTCTTTAATAAGACTGAACTTGGCTA TGGTGTCTTTTCACAGTAAAATGGAAACATAGACAAGGTTCTAACTCTCTGTACTAGAGGACCATCTCTA TGTCCTTGGTGTTGTATGTAAATTATTGAACAAATACTATAGACAAGTTGTATGGCCAAAAGTCCATCCC CTTCAACAATCTACTATCTGGAACCATATAGATTAGATTCCTTTCCTGCACTCATTTCCCTTACTTGCTG AGACATTTTGAAGACATGCTCAGAATCCCTGAACTTCCTGCTGAAAAAAAAATACCCCTCCAATTTGAAG ACAGTTCTCTTCCAAAATTCATCATAAATACTGATCCACATGTCCAGGCATATCATGTAGTTTTAAAAAA CAAACCAATCAATGCTAAAGTGGGTAAGTGCCTACATCATTGGTTTATGTCTGTTCATAACCTGGTATAT AGCTAGAGACCAGGACTGCATGTTTCTCCTAGGCCACACCTCTCCCACAAATGCTGGCTCTGCCTCTCCA GAATTCCAAGATCATGGATCTCTGGGTCCCCAAGGAACAATAAGTGTGCACTCTTGCCAGCCTGTACACT ACTGCACTAGCTTCTCCCCCTGGAGCTTGGTCATTGCTCAATCTTCCCTTTCCAAAGCCTGGCCAAATTC CTCCTACCTTTTGTTCTTTCAGTCAGGCCACCTAAGTCATCTTGAATGAAAAACAACACAAAATCTGAAC TTAAAATCAACAAATAATACAAGTGCTGCGCACAAAATCAATCATTCTAAACTCATCAACTATTTTATGA TGGAAATCTTCCAACATACAAGCTACCACCAGGTCTAAGAATTACTCATCTACATGTTGCTTCCTCTCAC TCACAGCCTAACCATAAAAGGGCTGTTTCTTCCTCCCATGTCCCCTCTTTTGCCTTCAGAAGCAGAAGCT CCACCTCCTCCTCTTTGCCCAGCAATTGGCTTCTTGTCGTCTTTATTATCATATTAATTACTTAGGGGAA AATCCCGTGTAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGG AAGGCTCACCAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAG CCATAGGGGCTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACG CCTTTCATCTGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAA TATAAGGACATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTG AGTAACTGCTAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGA CTCTAAGTCATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTG ACTAATACAGAAGTTGGTACCAGGAGTGGTTCTAGAGTAACAGAAGTACAAGGATGAATCTTTTAAAATA CTGGAATTGGCTTGTTGATCCACCAGCACTTTCAACTATTGAAACCTCTCCAGATTCTCTCCCTCCTGGG AGCTCAGAGAATTTTGAAGACCCATGGTTGAAACTATATTCCGAACTTAAAGAAGCAAATGCCCTTGATT TTCTTAATGAATTAGGTGATTCAGTGCACAAAGCTTTCTACAAGATGGGGAAAAAATTGGAAAATGATTT TACTGGCTGGCTGCTCTTAGTATCTGTGGAAAAAATGATGAATGAAAGGAAGGAGTTGTGTGATAAAATC GAAAGGCTCCAGACACAAGTAAACGATCTAAAAGTTGCTAAGTGTGTCCTTGAGGAGAATCTTCTCTCTT GTAGCAATAGAGCTCAAGTTGCAGAAAATCAAACAGAAACTCTCATTGTAAGGTTGGCTGAACTACAGCG AAAATTCAAGTCTCAGCCTCAGAGTGTGTCAACAGTTAAAGTAAGGGCTCTAATTGGCAAAGAATGGGAT CCTACAACATGGGACGGGGATGTGTGGGAAGACCATGTTGAAGCTGAGAATTTTGAATCTTCAGATTCTC AAGGGTTTGCCCCACCTGAGGAAGTAGTACCCTCAGCCCCACCCCTTGAAATAATGCCTTCCCCACATGA GGAAATTAATTTTGCAGAGTCTGATAAACCAGCAATGATTTTCACTACTGATGTTTCTCAAGGCCCACCA ATAGTTTCTTCTAGACCTGTAACCAGACTCAAAGCAAAACAGGCTCCTAGAGGGGAGGTAGAAAGTGTAG TCCATGAGGAAATTCGCTACACTACTAAGGAGCTTAATGAGTTTGCTAATTCATTCAAGCAGAAACCTGG TGAATATGTGTGGGAATGGATTTTAAGGGTGTGGGATAAGGGTGGAAGGAACATAAGACTAGAGCAGGCT GAGTTTATTGACATGGGTCCTCTGAGTAGAGATTCTAGGTTTAATACGGAAGCTCGCATAATTAAAAAAG GTGTCAAAAGTTTGTTTGAATGGTTGGCTGAGGTGTTTATCAAAAGATGGCCTACTGGAAATGACTTGGA GATGCCTGATATTCCGTGGCTTAGTGTTGATGAAGGGATTTTAAGACTTAGGGAAATTGCAATGCTAGAG TGGATATATTGTGTAAAGCATAATTGTCCACAATGGGAAGGTCCAGAAGATATGCCTTTCACTAGCTCTA TAAGACGCAAATTGGTGAGAGGGGCACCAGCACATTTGAAGGGTTTTGTTCTTTCCCTTTTCCTTGTACC AGATCTTAGCATTGGAGATGCTTCTGCTCAATTAGATGAATTAAATTCACTGGGTTTAGTTGGATTCCGA GGTAACAAGGGCCAGGTGGCAGCATTGAATCGCCGGAGACAAGGTGATTCTAGTTATTATAATGGACAGC GTAGACAAAAGAATGTTTATAATAACATACCCAGCAATGGTCAGCACAGGAGAGGTGAAATTTATAATGG CATGACTCGGTTGGACCTTTGGTACTGGCTAACCAATCATGGTGTTTCCAGGAATGAAATACATAGGAAG CCTACTGCATATTTGTTTGATCTGTATAAGCAGAAAAATTCTCAAACAAATGAAAGAAAGGCTACATTAG ATCATGGTAAACAGCAATCTCGGCCAGTGAATCAATTTCCAGACTTGAGACAGTTTGCAGATCCAGAACC CCTTGAATGAAGGGGTGGCCAGGTTCCGCTGAGGAAGGATCTTGATAAGACACCCAAAGGTTTTGCTGTT ACCCTTTCTCCAGTTCTTCCCCAGAGGGACCTAAGGCCTTTTACAAGGGTAACTGTACACTGGGGAAAAG GAAATAATCAGACTTTTCAGGGTCTGCTGGATACTGGTTCTGAGTTGACACTGATTCCAGGGGATCCCAA GAAACATTGTGGCCCTCCAGTTAAAGTAGAGGCTTATGGAGGGCAGGTGATTAATGGAGTTTTGACTGAT GTCCGACTCACAATAGGTCCAGTAGGTCCCCAGACACATCCTGTGGTGATTTCCCCAGTTCCAGAATGTA TAATTGGGATAGATATACTCAGAAATTGGCAGAATTCTCATATTGGTTCCCTGAACTGTAGAGTGAGGGC TATTATGGTTGGAAAGGCCAAATGGAAACCTTTAGAGTTGCCTCTGCCAAAGAAAATAGTGAATCAAAAA CAGTATCGTATTCCTGGAGGCATTGCAGAAATTACTGCCACTATCAAGGACTTGAAAGATGCAGGGGTGG TGGTTCCCACCACATCTCCGTTTAACTCTCCTATCTGGCCAGTGCAGAAAACAGATGGATCATGGAGAAT GACAGTTGATTATCGAAAACTAAATCAGGTAGTAACTCCAATTGCAGCTGCTGTACCAGATGTAGTTTCA TTACTTGAGCAAATTAACACATCTCCTGGCACCTGGTATGCGGCTATTGATCTGGCAAATGCCTTCTTCT CAGTACCTGTCCATAAGGACCACCAGAAGCAATTTGCTTTCAGTTGGCAAGGCCAACAGTATACTTTCAC AGTTTTGCCTCAAGGATATATTAACTCTCCTGCCCTGTGTCATAATTTAGTTAGAAGGGATCTTGATCGT TTGGATCTTCCACAAAATATCACATTGGTGCACTATATTGATGACATTATGCTGATTGGACCAAGTGAGC AGGAAGTAGCAACCACTTTGGACTCATTGGTAACACATATGCGTATCAGAGGATGGGAAATAAATCCAAC CAAAATTCAAGGACCATCTACCTCAGTGAAATTCTTAGGAGTCCAGTGGTGTGGAGCATGCAGAGATATT CCTTCTAAGGTGAAAGATAAGTTATTGCACCTGGCCCCTCCTACAACCAAGAAAGAAGCACAACGTTTAG TGGGTCTATTTGGATTCTGGAGACAACACATCCCTCACTTGGGTGTGTTACTTAGGCCTATTTACCAAGT GACTCGGAAAGCTGCTAGCTTTGTGTGGGGCCTGGAACAGGAGAAGGCCCTTCAACAGGTCCAGGCTGCT GTGCAGGCTGCACTACCACTTGGACCATATGACCCAGCAGACCCGATGGTACTTGAGGTGTCTGTGGCTG ATAGAGATGCTGTTTGGAGCCTCTGGCAGGCCCCTGTAGGTGAATCACAGAAAAGACCTTTGGGATTTTG GAGCAAAGCTCTACCATCATCTGCAGACAACTATTCTCCCTTTGAAAAACAGCTCTTGGCCTGCTATTGG GCCTTAGTGGAAACTGAACGTTTGACAATAGGACACCAAGTTACTATGCGACCTGAACTACCCATCATGA GCTGGGTACTATCAGACCCTGCAAGTCATAAAGTGGGACGCGCACAGCAGCAGTCTGTTATCAAATGGAA GTGGTATATACGTGATCGGGCCAGAGCAGGTCCTGAAGGCACAAGCAAGTTACATGAAGAAGTTGCTCAA ATGCCTATGGTTTCTACTCCTGTTACACTGCCATCTGCTGCCAAACATGTGCCTATAGCCTCATGGGGTG TTCCCTATGATCGACTGACCGAAGAGGAAAAGACTAGAGCCTGGTTTACTGATGGCTCTGCACGTTATGC AGGCACCACCCAGAAGTGGACAGACAGCTGCAGCATTACAACCCCTTTCTGGGACAACCCTGAAAGACAC AGGTGAAGGGAAATCTTCACAGTGGGCAGAACTTCGGGCAGTACACATGGTATTACAGTTTGTTTGCAAG AAGAAATGGCCAGATGTACGATTATTCACTGACTCATGGGCTGTAGCCAATGGATTGGCTGGATGGTCAG GGACTTGGAAAGATCACAATTGGAAAATTGGTGAGAAAGACATCTGGGGAAGAAGTATGTGGATAGATCT CTCCAAATGGGCAAAGGATGTGAAGATATTTGTGTCCCATGTAAATGCTCACCAAAAGGTGACTTCAGCT GAGGAGGAGTTCAATAATCAAGTGGATAAGATGACCCGTTCTGTGGACAGTCAGCCTCTCTCCCCAGCCA TCCCTGTCATTGCTCAATGGGCACATGAACAAAGTGGCCATGGTGGTCGAGATGGAGGTTATGCTTGGGC TCAGCAACACGGGCTTCCACTCACCAAGGCTGACCTGGCTACAGCTGCTGCTGATTGCCAGATCTGCCAA CAGCAGAAACCAACACTGAGTCCCAGATATGGCACCATTCCTCGAGGTGACCAGCCAGCAACCTGGTGGC AGGTTGACTACATTGGACCACTTCCTTCGTGGAAAGGACAGCGTTTTGTTCTTACTGGAGTAGATACTTA TTCTGGTTATGGATTTGCCTTTCCTGTACGTAATGCCTCTGCTAAAACCACCATTAACGGACTGACAGAA TGCCTTATCTATCGTCATGGTATTCCACACAGTATTGCTTCTGACCAAGGAACTCATTTCACAGCCAGAG AAGTACGACAGTGGGCCCACGATCATGGAATTCACTGGTCTTACCACATTCCCCATCATCCTGAAGCAGC TGGTCTGATAGAAAGATGGAATGGCCTTCTGAAGACGCAGTTACAGCGCCAATTAGGTGGTAACAGCTTG GAAGGCTGGGGTAGAGTTCTTCAGAAGGCAGTATATGCTTTGAATCAGCGCTCGATATATAGTACAGTTT CACCCATAGCCAGGATTCATGGGTCCAGGAATCAAGGGGTGGAAAAAGGAATAGTTCCACTTACTATCAC TCCTAGTGACCCTCTAGGAAAATTTTTGCTTCCTGTCCCCATAACTCTAGGTTCTGCTGGCTTAGAAGTT TTGGCTCCAGAGAGGGGAGTGCTCCTACCAGGAGCTACAACAAACATTCCATTGAACTGGAAGCTCAGAC TTCCCCCTGGTCATTTTGGGCTTCTAATGCCCTTAAACCAACAGGCTAAAAAAGGAGTAACAGTGTTAGG AGGGGTGATAGATCCAGATTACCATGGGGAAATTGGATTACCTCTTCACAATGGTGGTAAGCAAGATTAT GTCTGGAGTGTAGGAGATCCCTTAGGGCGTCTCTTAGTACTACCATGTCCTGTGATTAAAGTCAATGGGA AACTACAACAGCCTAATCCAAGCAGGATGACAAAGGACACAGACCCATCAGGAATGAAGGTATGGGTCAA TCCTCCAGGAAAAGAGCCAAGACCTGCTGAGGTGCTGACTGAAGGAGAAGGAAATATAGAATGGGTAGTA GAGGAAGGTAGTTATAAATACCAATTAAGGCCACGTAACCAGTTGCAGAAACGAGGATTATAAAGTAATA TGAATGCCCATTGTAAATTTACTAATGCGTTTGCGATTGTACGAGGGATAGTTATATCATGTTAGGCGTA TTTACAAACTTGTTATTGTTTTATGTGAACATGAGATATTATTTGTGTCAAGTTGACAAGGGGTGGATTG TAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGGAAGGCTCAC CAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAGCCATAGTGG CTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACGCCTTTCATC TGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAATATAAGGAC ATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTGAGTAACTAC TAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGACTCTAAGTC ATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTGACTAATACA TCCCTACATACAGGAAACTTGATGTATAAGTAAAGAAAATAGTAAATCTAAAAATTTCCTTATCAAATAC ATGCAAGAAATCCAAAATACCATGAAAAGACCTGATCTAAAAATAGTAGGAACTGAAGGTGAAGTGTCCC AGCTCTGAAAACTAGAAAATGTTTTCAATATTTTTCCTAACAGTGAAGACATGGATCTAATGAGGCCACC TCTTATAGCCATGCAAGACTGACAGTGGAGGGATAAGGACACCAATCCACCCACAAAACTTTTGACCCTA AATCAGTTTTATCTAAAAGCAATGCAGGGGCACAAATGGAGCAGAGACTGGAGGAATGGTCAAACAATAA CCTGTCATATCTGAGACCCACCTAATTGGCATACACCAGTCACTGACATTATTAATAATGTTCCATTATG CTTACAGGCATTTCTCTAGCATTACTATTCTTGGAGAGACTTCACACAGCATCTTATTGAAACAGATGCA GACACCCAGAACCAAACATTGGATGGAGATTGGGATCCTTAAAGAAGAGTTGGGAGGATAATTGAGAGAC CTCAAAGGAATAGCAACCCCATAGAAAGACACGAACAGGGTCAGTAAACCTGGACCCATGGGGTATCTCA GAGACTGATCACCAACCAAAAATCACAGAGGGCCTGGATTGATTCCCCTGGCACACATGTAGCAGAGGTC TGCCTTGTCCTTCAGTAGGTGAAGATGAGCCTAAGGCTTCAGAGACTTGATATATCAGCATACAGCAACA CCCAAGTGTGTCCCACTATCTCAGAGGTGAAGGGGTGGAGATGGGCAGAAGGGTCCTGATAGGGGACAAC TGCAAAGAGGCAATATTTGGGACAGAAAGAAAGGAAGAGAGAGGAAGATAAAGAGAGAGATGAAGGAAGG AAACAAACAAACCAAGATGAAGAGAGAGAAAAGGGGGAAAATAGCCTTGAATGGTTCAGGGATTCAAAGG GACATACCTAAGCATGGTAAAACAATGCACATAATGGCAGCAAGTAGCTAACACTGAATTAAATAGAAAG ACACATAAAGCAGTTCTACTAAAGTAAGAGACAGAGTTGCCCAATCTCACCCTATTTATTCAATAATAAT GTACTTGAAGTTCTAGCTAGGGCTGTAAGACAACTCAAGGAGATCGGAGGATACAAATTAGAAAGAAAAA TTCAAAGAATTGTAATTGGTAGATGATAGGATAATATACATAAATGACCTCAAAATTCTTCCAGAGAACT CTGAAAGCTGATAAATACCTTCAGCAAAGTGGCTAGAAACAAAATTACTTCATAGAATTCAGGAGCTATC CTTTATAGAAAAGTTAAAGTGGCTGAGAAAGAAATTAGGAAAATCACATTCTTTGCAATAACCAAAAATA ATATGAATTAGCTTGGTGTGACTCTAATCTTGCAAGTGAAAGATGTGTGTGACAAGAGCTTCAGGACCCT GAAGAAAGAAATCAAAGAACTCAAAGATGCAAAGTACTCTAATGCTCATGGATAGGCAGAATTAACATAA TGAAAATGCCAAATTTAACAATTCAATCTACAGATTCAGTGGAATTCCCATTAAATATCCAACCCAATTT ATTACATTCTTGAAAAAGCAAATCTCAACTTCATATAGAAAAACAAGAAATTGAGGGTAGCTAACAAAAT CCTGAACAATGAAAACACTTCAGGAGAATTCACCATCCCCTACCTCAAGCTGTATTTTAGAGCAATAGTT ATTAAAACTGCATGGTATTTGTATAGAAACAGATATGATGATCAATGTAATTGAATTGAATACACTGACA TAAAACCACACTGTTATGGACACTTGATTTTTGACAAAGAACCCAATAATCATAATAAAAATCATAATAA GAATGCATCCCCCAACAAATGGTCCTGATCTAAATGCAAATAGATTCATATCTATCATCCTGCACGAAAT TCAAATCAAAGTGAATTGCAGACTTCGACCTAATACTGGATTAACTAAATCTAAATGAACAAAAAGTAGA GAATAGTTTTGAACTCATTGATGCAGGATTCAATTTCCTGAACAGAACCACAATGGCTCAGGCTCTAGGA TCATAAATTGGTAGATAGGATCTCATGAAACTGAAAAACTTCTGTAAGGCAAAGGCAATAAAACAAAATG GCAACCTACAGATTGGAAAAAAAATAATCTTCAGTATCCCTAAATCCCATTGAAGGCCAATATGCAAAGT ATATAAAGAACTCAAGATGTTATCCTCCACAAAACCAAATAAATCAATTAAAAATGAACTACAGAGCTAA CAAGAGAATTCTCAACAGAGGAATCACCAATGACTGAGAATCACTTAAAGAAACATTCAATATTTGTAGT CACCAGAGAATTGCAAATCAAAACTACCCTGAGATTCTACTTTATACCAATCATAATGGTCAAGATAAAA ATTCAAATGACAGCGCATGTTGGTGAGGATGTGTATACTTTTGCATTGCTGGTGAGGAAAACAATCTGGC AGCTCCTCAGAAAATTGTAAATAATTCTACCTGAAGATCCAGCCATACCACTCCTGTGCATATACATAAA ATGTGCTCCACCATACCACTAGGAGATATGTGCCACTATGCCCATAGCAGCCTTATTTGTTAATAGCCAA AAGATGGATACAACCCTGATGTGCCTCAAACAAAGATTGTATATAGAAAATATGGGTTCCCTTACACAAT GGTATTCTACTCAGCAATTAAAACGTGAGAACATCATAAAGTTTTCAGGCAAACTGATCAAATGAAAAAG TATAATCTTGAGTGAGGTAACAGAACCAAAATGACCTGCATGGGATGTACTCACTGGTAAGTGAATATTA GACTAAAAGTATAGAATATCAATGATGAAAGCCACAGACCATAAAGAGTTTAATAAGATGGAAAGCTCAA GTACGGATTCTTCAATCCAATATAGAAAGAGGGACAAAATAATCAGGGGAGGCAGAGGGAGAGAGAGACA TTTGGTGGAAATGGGAGAAGAAGGGAGAAAGGCAGAAAGGATCAGGCGTTGGGGAACACTGGAAAGCAGC CCAGGGGTCCTGGTAAATGGATTAAATATTCAGCTGAATGGAGGTGGGAGGCAGGGGGAAGCTCTGCAAA GTCCAAGAGACCTGGGATGTGAGAGGCTCCCCAGATGATAATCTTAGCCTTCATGCCTAACAGTTGTAGA TAAACCCTGAAGAGATCACTTCCAATAGATGCAGAGGGCCCTAAGTGGTTGGATGGAGTCCCCCAACTTA CCTCAAAATTGTCAATACTCATAGAAATTAGGACAATGAAAATCAAAACACCCTGAGATTGTATCTTATG TCTGTCACAATGGTTAAGACCAAAACCTCAAGTGATGGCTTGTGCTGGCAAAGATTCAGAATAGTAAAAG TCTCCCCATGGCTTGTGGGAATGCAAACTCTTACAACCTCTTTGGAAGTATATTTGATTGTTACCTGGTA TAAAATGGCAAAAAAAAAAAATGGTTGAAGGGGGACTAAAAAAGGAAGAAAGGGGAGAACTATGGGATGC GAAACAAGAAAGTTTGTTGCAAAAGAAATATGTTTCCACTGCAAACCCTGAGTCTCAGACAGAAGGGGAC CTGGAATTCTTCAGATACAAAGAATCTCTAAACCCTGAGGACATTCTATCACAAATAAGTAAAATTCAGA AAATTCTGAGTGCTCCCATCACGGAGATGAATCTGCTATGAACAGCTCATAGGTGTGACCCTCTACAAAA GCCATATTATTGAAAAGCCACATTGTGCCCAGACTTTGGAAAGACTGAGCTCATATCCTGAAATACAGTT ATGTGTGGTTCTATTTAATTACACATTTACACTAAGAAAACATGGCAGTATGGGAATGAAGCTTGTTCTG TACACATTAACAGAGGGAAACTAAACAAAGTATGGTGAATCCCTAACCAAAAGTAAAAAAAAAAAAAGAA AGAAAAGAAAATAAAAGTGAAACTACAATATGTTTCAAATGCTGTAACTGAAATCTGGTTTTTTGATGCT TATATCTGGTATCATCAGTGACTTCAGATTTAGTCCAACTCCAGAGCATGGTATAGCAGAAAGACATGCA AATAAGCCTTCTCTCTGCCCATGAAAAACACCTCGGCCCTGACCCTGCAGCTCTGACAGAGGAGGCCAGT CCTGGATTCGATTCCCAGTTCCTCACATTCAGTGATCAGCACTGAACACGGACCCCTCACCATGAACTTG GGGCTCAGCTTGATTTTCCTTGTCCTTGTTTTAAAAGGTAATTTATTGAGAAGAGATGACATCTGTTGTA TGCTCATGAGACAGAAAAATTGTTTGTTTTGTTAGTGACAGTTTTCCAACCAGTATTCTCTGTTTGCAGG TGTCCAGTGTGAAGTGAAGCTGGTGGAGTCTGGGGGAGGCTTAGTGCAGCCTGGAGGGTCCCTGAAACTC TCCTGTGCAACCTCTGGATTCACTTTCAGTGACTATTACATGTATTGGGTTCGCCAGACTCCAGAGAAGA GGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTATTATCCAGACACTGTAAAGGGCCG ATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCCGTCTGAAGTCTGAGGAC ACAGCCATGTATTACTGTGCAAGACACACAATGAGGAAATGTTACTGTGAGCTCAAACTAAAACCTCCTG CAGAGCACCCAGGACCAGCAGGGGGCGCAGAGAGCACATGGAGTTCTGATTCACAGAAGAGTTACAGCCT GTACAATTAGACCCAATCTTCAACAAACCGTCAAAATATTCGATCCAAAATTGTTCCTGTCTAAAAGTAA TTCAAGGACAAAATGGACCAGAGACTGAAGAAATGGCTGACCTGTGACCCTCCCAACTTTGGATCTATCT CATAGGCAGGTACCAAACCTTGACATTTGTCACTGACACTGTATTGTGCTTGCAGACAGGAGCATAGCAT GGCTGACCTCTAAGAGGCTCTGCAAGCACCTGAATGAGACAGATGTAAATAGAGTGTACAACTGGATTAA GTTTGAAGACTGCAATGGAAGAGTTAGAGGAAGGAATGAAAAATCTGAAGGGGATCACAACCCCAGAAGA AGACCAACAGTGCCAATTAAACTGGATGCCTGGGAACTCCCAGAGACTAAGCCACAAACCAAGAATCATG CAGGCTGGTCTGAGAATCCTGGCACCCAAGTCTCAAGGAACTGCCTTTTCTTGGCTCAGATGGGAGAAGA TGAGCCTAAAGCTTTAGACTTGACGCCCCAGGGATGGGCAATACAAAGTTGAGTTTCTTGGGAAAGTGAA GAGGATGGTGGGAGGAGGAATAAATCTGGGAAGTGGGACTGGTTGGGGACAACATATTGGTCCTAAATTT ATGAAATAATTATCTAATTGATAAAAAGAGTCCTTGGGGTAATGGAGGGCTAGACTCCTCTGTGCCTAGT TTGTAACTCTACAGGGATCCTCTTTAAAAGAATAGGGTGCACATAGAGTATATGTGTGTGACACTAATAC AGGGGTAAGTGTTTCTGTAAGAACTTTATGAATAAACTTTATTAAAACATCAAAAAGTATAGGTTGTAGC AACCCTTGACCTGTACGAATGTTTATAAAACTTTCTATTTTCCTTAATTATATCTGTTTTGCATTTGTTT ATTTATTTATTTACTTACTTACTTATTTATGATTTCACTGTGTCTTTCTGTAGTCCTGTATGTGTTTATT TGTCTCTTTATTGCTCTGTTTCTGTTTCTTTCTCTCTGTCTAGTATTGTATCTCTCTGTGTCTCTATGTC GCCGATTGTGTCTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCATATTGAAA TGAGATCTCTTCTTCTATCAGTGTACATAATGACTTTGAAATAAAGTCCATCACTGGGCTTGGATATCAC TATCTTGTTAGAATGGTTGCCCAAAGTGTTCCAACCATCACACTGTTTCTACATATAATAGATGGGTTTA TTGATTTACTACCAAAACTACCCCATCTTAAGTTATGGATATTAAACACAGATCCTCATGGTGTGTTAAG AACTTGAAGCACAGAGCTATATGTACAGCCCACTAAAAATGACTTAAATATAAGATAAACTCTGGCTTGA ACAAAACACAGAGCATCACACGAGGCAGAAGTGAATCTCTATGAGGACCAGGATCACATCCCAAAGAACC AAAGAACACTTGAGACTGTGGAACCCTAGAGTCAGGGCTGACACTCAGTAAGATTTACTTCATAGAGCAT GATGTGGGATTCAAGAAGAAATCAGGCAGGGCCTGTGACCCATGAACCAGATGACTTCCAGTCTCTCCAT CATCCTCACACAAACCATAGGCAACAGCTCAGCACTGACTCACCTGTGATGCTGGTGACAAAATGTGTGC AAAGCCTCAGAACTGAGACGTGAGGCCAATGTTGCTACCGACACTCAGTGAGCTTCAATTTGCTGCAAAC TGCTCCAAAACAACTCAAAGGGTAACTTCTTCTTTTTCTATTTGTACTTGTGATGCCTTGGTAGATATAG TTCCCCAATAATTTAATAGGACAAATCATTTTTCCTTTTAACACATTAATCCCCTTTCCTTCCCCCAAGA TTCTAAAATTTTTCTTTAACACTGATGTCTGAGAGATATGGCATAATAGATGTTTGTCTTATTCTGAGTT GTTCCTTATCAAAAGAAATCAGCTGTGGCACTTTGAACCTAAATATAACAATATTTCACAAAGGCATTCT GGGAATATATAGATGGTATTGCTACAAAAGAATAGGAGGATCTATGGCCATACCACCCTTAATGAACCAG ATACTATCTAAAAGATTAGGACAGCTGGGCATGTTGGCACAGGCCTTTAATCCCAGCACTAAGGAGGCAG AGGCAGGCAGATTTCTGATTCGAGGCCAGACTGGACTACAAAGTGAGTTCCAAGACAACCAGGGCTATAC AGAGAAACCCTGTCTCGAAAAAACAAAACAAAACAAAACAAGAGTAGGAGGGTATTAGATGCGAGGTGAT GATGGCCATTCAATCAAACCAGAATGTGACCTGAGAAAAGGTCTAAGTTTGTCAGCTTGTCCTGGAGATG TGTTGCCTCTGATAAATGCAGAGCTGGAACTGCAATACATTGGATTCCTGTGAGTTACGGAGAAATAAAA GGAATAGCCTCTGTTTCCTTTGGTTCATCAAGTTGGCAGTGAGCTAGAAGATGATACTGGGAACCATCAC ATGTACTTCTACAGAGTTATTCTACATTCTACAAGTCAGTCTTGCTTGCTTTCGTTTCTGTGAGGAGCTA AATACAGAATGTTTTCAATGTCTCATAATGTTCTGTGCACACCCATGGGTGCCTAGCCAAGGATAGAAAC TTGTATCTGATAAGGAAAGAGGATGCTGCAGTGGCCACACTCTGAGATCACCTCCTGCCAAATTATACTA GAGTGTTTTACATAGTCTTGAAAACACTAGGTCAAATATGTTGTCATCCAGATATGAAAACTCTGTGCAA CAATTCAGAAATACAGTGGTCAGGACAACTGGTATGTAACACTTTAGTTCCAGCCCACATTAATCTCACA TCTTTTCTGTGGTTTCAATAAAGAGCACACATGTTTGGAACACCATCTCCACTTTGTATGCTTATCCTTA TGCATCAAACTTTTCCTGTAATTTCCTACCCCGCACAGGTTCCTGTCTTGATCTCTTTTTCTAATGAACA GCAATACAGAAGTGTTGGCCAAATGAACACTTTCACCCCAAGTTGCTTTGCCATTGTGTTTCATCACAGC ATTAGTAACTCCCAGTAAGGCTACTGGTTACCCTGCATCTTCTATGTAGATCTTTTGATGAAGTTCCAGG CTTGGGGTCTTTATCTGACTGTTATCCTGGTTAATTCATGTCCACCAATAGAATATTGTTCTCATTTTCA ATATCATATGAAGTTACCTGTTTATAGGATCTTCCTAAAGCAGAAAACTATATAAAATGTCTGCTTACTT TAAGAAAAGTATGTGTCCTTGGAGGTAAATGGAGGAAAGGGTGGAGGATGTTGAGTAATGGAAGACTGGC TAATACAGGAAGGTCACAGAATTGAATGATTTTAAAAATTTTAACTCCTCATCAATGAGGTACTTCAAGT TGAGGCAGGAGTCCTTGATGCACAGGTGACCCAAAGGCCCCAGCCTCTGTTTCTTGGTCTGCTCCAGTCT CCCGGCTCAGCACAGTTCACTGAGTGACAATCTTTAAGATAGTCTTTCAGGATACAGTTATCACAGTTTT CAAACTCTCTTCATTGAAACAATATAATGAGTCAGAGTCCAGTGTGCCATTAATAGGCCCTATGCAATAG GATTTTACTTCATCTGTGGAAAGAAACCACCATGGCCTCATCAAGGCCTCATTTGATGATTAGTGTAGAA AGCGTCCCCAGAGCTTTGCTGGGATGAAAACCCTGGCAAGATGTTTAGGGCACTCTCCATCTGACAGGAC AATTCTATCAAGGGTTGCTTCCATATAGAGAGAAATCTCCACATCTGCAGTATTTCCCGATTTATCAACA GTTCTTTACCCCGTGTCATATGCATAAAATCTTTTCTCTGTAGAATTTTCAGTACTAAAAACTAACAAGG ATGTTGGTCCTGATATCAGACATAATAAATAAATAAACATCAAATTATACCCTAAACATGTGGAGATGGT TTTCTGTTATGAATTTATTGGCTCTCTAAAAAGTGTTAATTAAGCCAGGCGTGATGGCACATGCCTTTAA TCCCAGCACTTGTGAGGCAGAGGCAGGCGAATTTCTGAGTTTGAGGCCAAACTGATCTACAGAGTGATTT CCAGGACAGCCAGGGTTACACAAAAAAACCCTGTCTCCAAAAAGTAACAAAAAGAAAGTGTTAATTAACA TCTACACTTTAAGATAATTTGGTGGCAAAGGTTAATAGTCTGAAATACTAGATATGAAAGTATGACACCT AAATTGCATGCAGAGAATAAAGCTGAATTCCAATGAGATTCATGCCTTGTGAATCCATTCCAATCTGATC TCAATCTTCAAACATGTTTGTGCATAATTTACATCTTAATCAGTTGCTGGAAATTAAAAGGACATAAGCT AAACTTAAAACTCTTTCTATTTAATGTGTCAGAATAGAATATGTGCACTGAAGTTTTCATGTGCATAGGA TATCATTGTTACAATGATGGTTCAAATACGCACCAGTAGCACAGGTGATGCTTGACATGAAAAAAACTGT GACCAGAGCCCAGACGTTGATGTTGGCTGAAAGAGCCTAGTGTCTCACATTTGATCAAGCAAAGTGTTAT TCAGAGAAGCAGTGCCAGATTTGGGATCCTTCTTAATGGATCAAGAACATGAGGAAGATTTGGAAGCCAG ATTGATGAGATTACTAGAATCCTGGATCTGAACACACAACACACAATAGTAAGTTGAACTTTTCCACGCA ATGCTAAAGATAAAGCATCCAGTATCATGTTATACAGGACCAAGGTGAGGATCAGTCTGTGGGTGAAGTA CAATGAACTGGAAATTGTTTGGATTTTAAATGTTGGTTTTTAAATTATATATAAAATATATATATATATA TATATATATACATATATATATATATATATATATATATATATATATCGGAACCAACATTTAAAATTTGGAT TTTAAATGTTGGTTTTTAAATTATATATAAATTATATATATATATATATATATATATATATATATATATA TATATATATATATATCGGAACCAACATTTAAATTCCTAATTCTTAAGGACTTCACTTTTGACCAAAAATA TTTGAAATGGGTTATTTAGGTCTGTGGTGGTTTGAATATGCATAGCCTATAGGAAGTGGAAGTATGGCCA TTTTGTAGTATGTGTAGCCTTGATGGAGGAAGTACATCACTATATGGTGTTGTGAGAGTTGAGATCCTAT GTTCAAGTTCTGCCAAAAACAGAAGAGAGTCCCCTCCTGGATATTTGAAACAGATGCAGAGGCCCAAAGC CAAACATTGGGTATATCTCAGGGTTTCTTATGGAAAAGTTGGCTGAAGGATTGAAGGCCTTAAAGGGGAT AGGAACTTCACAGGAAAACCAACAGATTCATCTAACCTAGACTCTAGGGGTCTTTCAAAGGTTGAGTCAC CATACAAAAAGGACACAATGGCTAGACCTAGGTCCCTGATACATATGTAGCAAATGTGCAGCTTAGACTC CATGTGGATTCCCCAACGACTAGAGCAGGTATTGTCCCTGAAGTTATTGCTTGTCTGTAGAATACATTTC CCAACATAATTGTCTTGTCTGGCCTCAGTAGGAGAGTATGTTCCAATCCTGGAGAGACTTGATATGCCAG GATTGAGAGATTAAGGGTCTCCCACCATCTCAAAGTTCATGGGTATGACAGGGGAGGGGCAGTGCAGGTT TTCCCCATCTCTGATTTCCAGCATTTGCACCCCCAATATTGAGGGGGAGGTCTAGCAATTGGAGGGGGGA CAAACAGTATGAGAAAGCTAACTTTAGTGGTGTTGTCACAGTGTGGGGTATTGTGGGTGGGAGTGGAGAT GAATCCTCCTCCTCCTCAGGAGGCTGTAGCAGGTGCTTGCCCTCTTTTGGGACAGTAGAGCTGTGGTCAG TACTGGCCTGTTCCTGACAAATTTATTCTGTGCTGTGACCATTTATTCAAGGGAGGAAGATTTAAGACTT GCTATGATAACCTGACGAATCTGGACAAACAAGAGACAGGAGGTAACACCTGAGTTACTCTTTTGCTTGC TAGTACAGAAGAGCACTATAATCCACAAATTGGGGTCCCACATATTCTTGAGTGGGATCCAAGGTGCCAT AATTTCAGCCACATTCCAAAGTCCCTCAAGGTCTGACAGTTTAGTCTTTAGGCTTCTCCTAACCAAATCA TATTTGGAGTTCTTTAACATAAAAGGGTAGTTTCCCCATATCACTGCCAGCAAAACAGGTTGAATTTGGA CAGACAAGCAAAACTATCACAAACAAGACAGACTATGTGTGCGATCCAAATGTCTTGTGATTAGAGGGGA AGTTCTGCAGCTTATTAGGCTGTGTCCTTATGCCCACAAATGTCTTCAGATAGGAAAGGGCCATTGGGTG CCAATCCTTAGTTCTTCTGTTTCCTGGGCTGTCAGTTTTTATGTGGAGAAGACATGAACCAAATGCAAAG TCATGTATCCTACAAGAACTGACATACACAGACAAACAAGGAATAACAATGAGCTCTCTCATTCACCTAA GGGGGTCTTATAACATAGAACAGAAAGGGGCCAACTTAGCAAGAATTGACCAAAAGGTGGTCACCTAGAC CACACCACAATGCAGGAAGGATATCAATGAAGGAAGAACAAAGGAAAGGCCAAATCAAGTTTGTTGGCAC ATGACATAAAACAGTGCTCAAAAAACAATAGATGCGAGCAAAATGAAGCATTGGAATCATATCGGGGTAC ATAGGGGTCACTCTTACCCCTGTTCAAGTGCCAGATGTTGTTTTAGCCTTAGGCTCCCTGAGTTTGTCTT TAGGGGAAATGGGTAAGAATACACAAAAATAATGTCACAGAATCTTAGAGGCAGATGAGAAGTCAAAGCA GACTCATTTATTGCTATAGAAAAGAATGCCTTTATACCTTCTGTCCATAACACCTCTGCCCATATATGCT CATCTCTGTGAGGCAATGGACTACCTGCAGACAGGCTGGTATCCAGTCAAGCTGAGGTCTGAACCACAAT GGTCGTAACTCACCTAAAGGACGTGGTAGGCATGACGCGATAGGCGTTCCCTCATGCTCCTGGAACTCCG GCCCCTGCCTAAGGTACCACCTCCCACAGCCCCCACAAGAGAAGCATGGTCAGTAGTCATGTAAGCAATG GCCCAAGCTTCTGACCTTCAGCCTAAACTCCTCCCCAGTTACCTAGCAACAGTGAAGACCATAAAATGGG ATGTTAGGCCCCCACCTCACTCTCTTACTCCTTTGTTCCTTTACCTCTCACTTCTCTCAATTCTCTCTCC TCTTCCCTTTCTTTGTCTTCTTCTTTCCTTGTCTCTCTGTCTTTCTCTTTCTCTCTAGCCTTCTCTGCCT CTCTCTCTCTCTCTGTCTCTCTGTCTCTCTCTTCTCTCTTTCCCTTGAATTTCTTTTTTTCTTTTTTCTT TTTTTGATGAGCACAGTATAAATAGTTTATTGCATTGTTTCTGAATGTGGCTGTATTCTCTATTGTTCTC TTTCTTATTTTTTTTCTTTTTTTGGTTGTTTTTGTTTTTATAGTCTCGCACAGAATTCTACATCTACCAA ATCCCCATTGTGTACTTTAGTTTTGTCTTGTATTCAAAGGTTCTTTCAGAAAGAATCTTAAAGATGAAAT TTGCCTTCCAACTTTTCAAAAATACATATAAAGATAGATGTTACATAGTTCACGGAATAGACACATATAC GGAGATGCAGCAATATCTCCTTTGTGCCTCTTGTATATTCATGTTATTTCCTCAACTAAAAATACATAGC CACAAAGACATACAATGAGCACTCCACTTAGGAAAATATGGCCATATTTTTTTACATTTTTATGATTTTA TGATTAGGTATATTCTTCATTTACATTTCCAATGCTACCCCAAAAGTCCCCCAACCCTTCCCTCACTCCC CTTCCCCACTCCCACTTGTTGGCCCTGGAGTTCCCCTGTACTGAGGCATATAAAGTTTGCAAGACCAATG GGCCTCTCTTTCCACTGATGGCCCACAAGGTCATCTTCTGATACATATGCAGCTAGATACATATACATAC ACGAGCTCCAGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTATAGGGTTGCAGATCCCTTCAGCTC CTTGGGTACTTTCTCTAGCTCCTCCATTGAGGGCCCTGTGATCCATCCAATAGCTGACTGTGAGCATCCA CTTCTGTGTTTGCTAGGCCCCATTATAGCCTTGCAAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTT GCTAGTGTGTGCAATGGTGTCAGCATTTGGGGGCTGATTATGGCATGGATCCCCGGATATGGCAGTCTCT AGATGGTCCATCCTTTCATCTCAGCTCCAATCTTTGTCTCTGTAACTCTTTCCATGGGTGTTTTGTTCCC AATTCTAAGAAGGAGCAAAGTGTCCACACATTTGTATTCATTACTCTTGAGTTTCATGTGTTTCCCTTGC ATTTCTATAATAAACCATAAGGAGTCTCTGCTCTACCAAGACCCGCTGCACACTCTGGTCAGTGTTGGGA ACTTTTCCCCTATTCCCTCTCTCCTATAACTCCGGGGCTACAGGGTGTCTCCTTTGGGTCCCGGTTGGGA GCTGTCTCTTCTCAACCCCCTGACTCATGGGTCAGAGGCCTAAATCTCCACCCAAGGCTGTGTGAAAAGC ACCGGGTGGTCTCCCCATATCTCCCTGTCCAGAGCACAGGAACTCTGGCCGGACATGGCATATTTTTCCT CCCCAACTTCTTCCCCGGCCTCCTCAGGGCTGTCCCTTCATTTTGTTCCCCACACATCTCCACATGGCTG CCACCATCCAATTGGCTGCTGAGGTCACACACTCTGTCTTTGTTCTTCTCTGAGTCTCATGCAGGTTTTG TGTTAGTAAATGCAAAAGTGCCTGCTGTGCATGCATGAACCATTACAGGCTGCTAGGCAGAACATGCTGA CTTGCTCCTGGATTATAAATCCATCACTGTGATAGCAGGACATATCCAGAAGACATTATTTAACAACACT CCTCTTCGTGCTCAACCTCTCATGGTCTTTCTACCTCCTCTTTCTTAGTGTTCTCTGAGCCTACATGAAA TGTGAACAAACCTGAAAAAAATCTCTGTGTCAAATACATAGTGTGGATTGGGAAGGAAAAACCCTGCACT TGTGTGTGGGCAGGGAAATGCTGGTGCTTTAACAGGACAAGTGCGGTGTGGCTAGAGAAAGTGATGAGAA GAAGGGAAGCATGAGAACTGGGTGGGTGGATCCCATGTCTACTGAAAATGCTATGTTGATGTCAAGATAT TGTGTCTTGTTACAAAAATTCATTGAGTACCTAGGTTATTCTCAGTATATAAAAGGGGAGGAATGAAACT AGAATTAAACACACACACACACACACACACACACACACACAAACACACACACACGAGTAGAGAAAAGAAA TAGTCATGAGTTGCCTAGTGAATGAAGAGCGGAAAATGGTGCAGTTAATAGGGATCATAGAAAGAACTGG GGGATGCACACGAATGGGTTTCCTGTAATTATGTCTTCATAGTAATCTCTGCTCAATAATCAGACAGTGA CAATGTATGCCTCATTTACAAGCCCTGATGGCCTCTCAGAGGAAAGCATCTCTCTGAAATGAATAAAGTT CAGAAATGTCCTAAGTGTCCCTGTCACAGGAAAGCAGTGTTGGTAACGTTTCCAGGAAGCTCAGTCTTTG TCAAATATCCACAACAAGAAGAAGCCATGTCTAGACAGACAACAGACTGGGAAAGACTGAGCTCATGTAT GGAAACACAACTATGTGTCATGCTTTTATTTTCACATTATACTGAGGGGATATGACATTATAGAAACACA GTTTGTTCTTTGCATATGCTGAGAGAGGAACTAGATGAAGTGTGCTAATTCTTAGCAATAAAAAGATATA CAAAGTGGTACTTTACTGTTTCAATTTTTGTAACTGGCATCATTGTCTTTGATATTTTATATCTTCCTTG AGCACTGCCTTCTCTCAAGTGTCCAACTTCAGAGTATGCTATAGCAGGAAGACTACCAAATAAGATTAAT TTTTTTGTACCCATGAAAAAATTATGTGCCCTGACCCCTGTTCTCTGAAAGAGGAGCCAAGTTCTGGATT CCCAGATCCTCATATTCATTGATCAGCAGTGAACACAGATCATTCACCATGGACATGGGGCTCATCTGGA TTCTCCTTATTGTTTTTAAAAAAGGATTTCATTGGGAAAAGCTGCCTCATATTTCTATGACCAGGAGAGG AAGATACAGCTAGAGACACAAGCCCCACCATGTAAAATCTGTATGGTACTCTCGTTTTCATTTTACAACA TTTTGCTTTTGCATTGCAAAGGAGCAATAAGGGTTCAGCAATCAGCACTGTAACTGTACTTTTAAAATTT CCACCTCTACCCTCTGTGTCCTCTATCTAGTGATAGAAAGTGAAATAAGCACATAAAGCATGGGACAAGA AGAACACACAGAGGAGCAGAGACTGGCTACAAGTGGCAGCCAGCATGGAGCTGGACAGAATTTAAAAAGA TAAAGAGAAATGTCATGGCATGAAGCAGTGACTTTTTCTGTTCACAATGCCTGCAAAAACTTTATGGAGC CTACTACTGCTGAGATATGCAGGTTTGGTTCCAGAGAGGATTTTCTGTTTTATTTAATTTGCTTTTAAAT TTTTCTTCTTTGTATAGTCTGTATTTTTAAAAAGGGGTTGAATGAATACCTAAGCAGTTGGAAAATTTGT GAGTAGAAACCGAGGGCCTGAGAAACAAATAAATCAAAATTTTGAAATGGCAAAAGAAAGGGTTCATTCT ATTTTCTCTTTTCAGAAAAGGTTGCAAAGTTGGATGTGCAAATGGAGATGTTATGGGTGGACTTGGAACA TCTTGGGAAGGAAGGAACCCAGGAAAAGAAAGAATGAAATGGCTCAGCCTCAATGACTGAGCAAGAGAGT TAGAGTCACCCACAGGAGAAAAGAAGAAAATAAACAGCACTGGGGTGAATTTGGGTCAGAGAAAACCTTA AAATGCCAGAGGACAATGTGATGAAAGAGACCATTTGAAAGAAAGGGTTAACCTCATTCCATCAGTCATG CCAGAGTTAGAGAATGGGCAGTCTGGAGTTACAAGATCACGATTAGCCTTCCCAGTATGTATACAGCATA TTCCTGATAATGAGATGAAGCAAAGTTTGACAAGGTAAGATTGTATCCTATAGTAATATATAGATCAAAG GCATGTTGCAGTAAATCTCCAACAGAAATAAGCCCGGGCAATGAAAACACAACTCAATTAATATGAATAC GTGCTGTGCACCTAGACTGGGCAGATCTACCACTCCACTACCATATGAGAGATCCCTTATAACTTGTGGT TTCTCCAGGCCAGCTGCTTCTGCTCTACTTTCCTTCCTCCTCCTCCTATGTCATCCTCTCCCTCACTCTT TCTCTCCCAAAACTTTCAGCTGCACCTTCCCCTCTTCATCCCCCAATCAGTGGCTCTAGCCTTTATTTAT AAATTAAGGTAGGAAGAAGGTTTATAGGAAATTACCTGAGTGTTGACGTGTTGATAACCCCACACAAGAG AACAAAATTAATATCAAATATGATTAGCTCCAGGGTTATATGCAACAAAGGCATGATAAAGTGGTCATGA TTTCATATGAGATGTACTGGACTCATGTGAAAAAACGTTTAAATACATGGGCTTACTCAAAATATAGACT CATACCCCCAAGATGGGAAGAGACTGGTAACATTTGTACTGGAGGCTGGTCTGCTGTGGCAGCCATTAAC ACAGTGGAGGGAAGAGCCTCAAAGAGTGAAATAGAGGAAGGGGAACAAATACAGTAAACCACCAGTTACT ACTTAAAGGGCAGTGTTCTGATGTCCTAGGGAGGGCACTCCAAAATACCAATGGCCTTATCCTTAAATTT ATTGTTTAATGAGCCTACACTGATCTCAGTGGCCCACTACTAAGGAGAAATGACAGGCACTTGAACAGCT GATATGAGAACAGCTGGAGGCACAGTATACAGAAGAGTTTACAAGCCTATGGAACTCCCCTGTATTTTTT TATGATGAGGAAATCTGAAAAATGTAAAATGTTGAAAGATTTAAGAGCAGTTATTAGAGTAGATCAACCA ATGAGTCTCTTGCAGCCTGGAATTCTTTTATCTTGTTTGTAACAACAATCAGGCTCCATAAATTATAAAC TATAAAAATTTACTTTATAAATTATAAATATATATATATGTGTGTCTGTAGATAGATAGATAGATAAATA GATAGATAGATAGATAGATAGATAGATAGATATATGGATTTGATGGTTGCAGCAAATTCTAATTGGTTCC AACAACCCTACTCACATAATACATTCATACAGGTTTGATAGATGGAGCTAAGTTTTAAATAAATTATTTT TCTCCCATGGCTTATATATACACCACCAAAATTCTCAAAAATTAAAATGTGATTGTGTTGTATTTTTCTT TTACTGAATGACTATAAAAAGTTACAACATTCTCCATAAATTTACATGAAAAAATATTATGTAGTGCAGG TAAAGAAAACAAATTGACCCAAGAATGGTGTATATTCATTACTAAGCAACTTTTTAGATTCACAAAGTGT GGGTAAACAAGGTAATTTTTTCAATCAGTTTTTTTTAACTGGCAGGCAGCAATTCAGAGTTACAATGAGA AGATTAATAATTTTATTGTGTATTTTAAAATAAATCTTACAAAAATATTAATAGAATCACAAATTTATAC CTTTGTATAAAAACAATCAGTCATTTCTACTTTAAGAAACAGAACTCACATCTACTCATCAATCATTTTA TTAAGTCATATTACAAAGCTGAGTGCTAACATGGGTATAAGAAAACCATAACCTTATTCACCAGCCCAGC GTCAAAAAGAAAAAAACCAGTCATATCAGCTGCTGCTTCAAGAGTTCTTGTTCCTTGACATTAACAAAAT CCCTAGCTTAACTATTAAATTTTTTTTCAAAACTTCTAATTGATCCCTTAGATAAATGTTTGTGCTAACC ATCGGGACACATCCCATGAGTTCTGAAGCAGTGTGTTGTTCTTCATGCATGGCCCTTTTGTAGAGCTGTG AGTGTAGGGGAAGAGGGGGGAGAGAGAGCCCGTGTCCAGCCAGAGATCCTGTGCTCTGGGCAGGCAGACA CGGGAGGACAACGGAACACTTTTCACTCGGCCTTTGGTGGGCATCTGGCTGTGTGAAGTTACTGACCCCA CATGGTGGGGGATGGACAAGGGGCAGCCCCTGGTACCAGGAGCCCCAGGGCTACACTCTCGGCCCCAGAT ATACAAGAAGAGGGCAGAGGGAGAGAGGCTCCCACACAGGCGAGAGTCCTTAGTCTGGTCTGTGGCTGGA GCAGGGGAATTCCTTCTGATTGGAGATTAGGCACAGCTGATTAGGGGAAAGCCTACCCCATCATCCAAGC ACAATGGACTTTGAGGAACAGAGCCAGTCTAAGCTTTTATAGCTTTATGGTAGAAAGGCAGGGAGAAAGG AGAGAATGTGGAGAGAGACAGAGAGAGACTGGCCATGGCCAAGAGGAGGGAAGGGGGAAGAGAGAGAAAC AGGAAAAGCTAGAAAGTAAGATAAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCAAAGTG GGGCCAAGCAGCCCCTTTTCTAGTGAGCTTGTCATCTCACAGTTGCTAGGTAACTGGGGAGGAGTTTAGT CTGAAGGTCAGAAGCTTGGGACCTTGTGTATGTGACTACTAACCACAGCTTCTCCTGTCGGGACTGTGGG AGCAGTAACTTTGACAGGAGACAGGGATCCAGGAGACATGAGGGAACACCTTCTGTCCCACATAGGTGGA ATCGGTTGTACCAGGGTTCAGAACTCAACTCCACTGGAGACCAGCCTATGTGTGCATAGCCCATTGCCCT ACAGCCTTTGACATTCTACAGAGTTCTGAGACTGGTAGAGATACAAAATAGCTAGGATTACACAGAGAAA CACTGGCTTGACAATAAAACTTCCAAAACAAATATAAATGAATAAATACACAAATTGGAGCAAGTGAACT TGCACTAAATCCATCAAAACTTGCACAGGATGGCTGTCTCCATAATGTTCAGATCTACTTCTATTAATGT CATTAAGAAGACAAAGATTATGCACAAAAAGAAACAAAACAAGAAACCCAAAAGGTAAAGGCTCCAATTG TAAGTAATAGACCCACAGTCTCTGAGGCCTGACTGTGCAGGTCCTCACCTTTGGTAATCAGCACTGAATG CAGACCACTCCTCATGGACTTTGGGCTCAGGTTTGTTTTCCTTGTCCTTATTTTAAAAGGTAATTCATAG AAATGAGATCCTGCCAGTATTGTGTACATGAGAAATAGAAAAATTGGTTTTCTTTGCTCTATTTTGTTTT GTTTGGTTAATGACAGTTTCCAAATCAGCATTCTTTGCTTTGAGGTGCCCAGTGTGAGGTGAAGCTGGTA GGGTCAGGGCAGCCTGGAGGGTCCCTGAAACACTCCTGTGCAGCCTCTGTAGTCACTGTGAGTGACTACT GAATGACCTGGGTCCTTCAGGCTCTAAAGAAGGGGCTGGAGAGGGTGGAAATAATTTTTAATGGTGGAGG TAGCACCTATTATCCAGACACCATGAAGGGCTGATTCACCATCTACAGAGATGATGCCAGAAACACACTT TACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG TGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTATTCATATATATATATATAGTGTGTATGTATATTCATAT ATATATGTATATTCATAAGGTTGAGTAAATGGACTTAAACTGATTCCATCACAACTTCCATGGGATGGAT ATTTCCCAGTGTTAAGACCTGTCAACATCACTGTCATTCAGGAGACAATGATTATGCACAACAAAACAAG AAACCCAAAAGCAGAGGACTCCAATTACAATAGACCCAGACCCACAGTCTCTGAAGATTGACTGTACAGT TCAACCCAGCCCTGTACTTCTCTTCCCTAGAATTTACATTACTGAGTAACTGAGGAAAGCTCTACAATAT CTGTTCTCTATAGTGGTCAACACCTCCAAACACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTT CTTGCCATAATGTAGACTACTTCAGCCTATTTTGTACTCCAGTTAACGAAACTCAAGACTAGCTGCATGT TAGTCCTTATTCTGAAATATTATGAACAGGTGACCTCCCATCATTCACCAATGCAATAATCATATTTAGG AATATGAGGTTTTATGAGATATAAGCACAAGGGAGAGAAAGTAAGAAAACTACATAGATATATAGACTGA CATAAATCAAGACTTGCATGAGCTAGTGCCCAAGTACCATGTCCCTAAGTGGCAAGGAGTATCTTTTGAG CCTAGTGAGATGAGGTACAAATCGGACTCTTACATCTTTTTAGATAAACATGTGAGATCAATGGACTAAA GGCGTGAGCTGGGCTTCCTTGCAATCCATTTTCCATACAAGATAACAATAGATCTGGCTCCACAGACACG ATGAGAATAGTCTTAATTAGTTCTTTAAGTAGAATGACTGATCACTAAGAGCCCAATTCCATACTAAATA CTCTTCTGGATTATGCACAGATAAAAATTGCACATAGGGCATGGGGCACTGATCTCCCTGCACTACATGA ATGGGGGCTCATTTACTAAATGTTCCCATGTTTCTTCCTAGTGCTGCACAGAGCAAATCCTACAACTTCC TGCTTGTCTACAATGTAAACTCCAGACAGTACCAGAAAATCATTCCTTATGTTCCTCTCCAGGTGCTTCA ACAAGCACAGTGCAAATTTCTGTCACCCTG Vaximmutor Vaximmutor MOMP VO_0010890 1518659 CDD:279628 83560 ? major outer membrane protein 4.86 39872.84 457 mouse pneumonitis; submitted as Chlamydia trachomatis >AAB07068.1 major outer membrane protein [Chlamydia muridarum] MKKLLKSVLAFAVLGSASSLHALPVGNPAEPSLMIDGILWEGFGGDPCDPCTTWCDAISLRLGYYGDFVF DRVLKTDVNKQFEMGAAPTGDADLTTAPTPASRENPAYGKHMQDAEMFTNAAYMALNIWDRFDVFCTLGA TSGYLKGNSAAFNLVGLFGRDETAVAADDIPNVSLSQAVVELYTDTAFAWSVGARAALWECGCATLGASF QYAQSKPKVEELNVLCNAAEFTINKPKGYVGQEFPLNIKAGTVSATDTKDASIDYHEWQASLALSYRLNM FTPYIGVKWSRASFDADTIRIAQPKLETSILKMTTWNPTISGSGIDVDTKITDTLQIVSLQLNKMKSRKS CGLAIGTTIVDADKYAVTVETRLIDERAAHVNAQFRF Protective antigen Female BALB/c mice were immunized with chlamydial major outer membrane protein (MOMP) mixed with cholera toxin and CpG oligodeoxynucleotide adjuvants by either the IN or TCI routes. Both intranasal and transcutaneous immunization protected mice against respiratory challenge with Chlamydia [Ref1061:Skelding et al., 2006]. PmpE/F-2 Chlamydia muridarum str. Nigg VO_0010893 1246432 497915819 TC_RS01330 AE002160 WP_010229975 243161 308276 311353 - autotransporter domain-containing protein 8.26 107782.38 1025 similar to GP:4376753; identified by sequence similarity; putative >NC_002620.2:308276-311353 Chlamydia muridarum Nigg, complete genome GTTAAAAGACCAGAGCCCCTCCTGCATTCATGTAGTGTGAGACTGTAGAAGTAGCCACCTGCGCTTGATA ATTAGCGAATAGTTTAAGATAAGAGAATTTTAGAGAAGATGATCCTCTCCCATAAAAGGAATGCTTAGCT AAAGGAGTGTTTGTTGTTTCCCAAGAACCATTGTTTTTGATTAAAACGGTATTAAGAATGGGGCGCTTCC AGTAAAGAGTAGGCTGGTATGCTACCTCCATTTCGCAGGATACAGTTGGCCATTTATTCGAAGAATAAAC ACCTTTAATTCCTATTGGAGAAACAACAGCTGTATGTGGTTGTTTTAACGAAAATAATCTAGCCAGGTCT CCCTGCTCCTGGATAGATGCAGGCTCTGTTCGGGAGATGAGAGCTTGAATGAATGGTGTCAACATAATCG ATTGGAAAGGCATACTATCCCGCAGTTCGCAACGTAGAGAGCCCCCCAGAGTCGTAGAGTGAAAGTTCCC TTTAGAAAACTTTTTCGTTCCATAGAAGTTATATGTTTTATGAGTACCATAGTTATAAGCAGCTGCTCCA ATAAGAGCGATACGATCAAACATAGGTTGTTCAAAACACAGAGCGGAGAGATAATAACGAGAAGAAATTT TATTATTGGTACGTTTCTCTTTCATTGTGTCGGAGGATTGGGAAAAGGAAAATAAAAACTTATGTCCGTG TGCTCCTGATGCTTGTGAAGATATAGCATATCCTTTCGATGCAGAAGAATATCCGTGATAATCTAAGCGT TTCTGCTGTTTGGTGAATAGCGTAGCTGCAGATCCTTGTAAAGCGAACCAAGAGTCATCTGGTAGTAATG AACGTAAGTACAGGGTTGTATTTCTTCCAGAAGACACTAAATTATTCGCAATAAGATCCCCTCTACGAGC AGGATCTGCTATATATCCGATTGGGGTCCAGCTCACTACTAAGGAGTGTCTAGTGACTGTTACGTCCGTT GTAGCAAGAGGAGTTGTTCCTCCTGTAGAAGCTCTAATGGAAGGAATCTTAGCTGTAGTTGTACTGTTGG AATCAAAAGTTTCGCTAGTGTTTTTTTGTTCTTGAATAGAATTGTTGGCTGTTTGAGTCTCTGTACTATT GGTAAGAGAGACCGTACGGGTGGTTGTCATCCAATTAGATGTCCAGAGTCCTTGGTATCCGTAATGTGGT AAAGAATATAGCGAGGTATTGAGAGTGTTTTTCCAATCATTGCTTAACTGCGCATCATTAGTCGTTTCGA TATGTAAAATGGGTTGATTTGCTTTTCCTCGGGATAGGTCGCAGCTGTCATAAACTGATTCGTAATTTTC ATCTACTAACGAGACAAGACCGGAGAAAAAGATCTTTTGAGTTGCCACGTTCTCTGTGCTTACTGGAGCT TGGTTAGAGTTTGTATTCTGTGTCATATCTTGGGGATTAACACGAATACGTGGAGCTGTAGTACCTTGGT CATTGAGAAGACCTGGCAAAATGATTCCAAGATGCGTAATAGTGAGAGAAGCGTCTTTCCCTACAGTAAG AATAGAATCTTGTCCTAAAGCAAGAAGGCTAGTGCTTTCTTGAGTAAGAGGGCCATTGAAAAATTCAAAC TCTGCGCCATTTTCTATGGACATAGTCCCAAACTTCAAGGTAGTGGGGGAGTCTTTAATTTGGTTATGAG TTTTACTGGCAATCATGTGAGCTTGTAGATCACTGGATATGTTTTTGTATGAAAACACAACAGCGCCAGA GAACTCATCCGAAGGATTGATGGTAACGGTGTGCGGGCTATTTTCAGGGGGTGTATTAGCTTTTTTTAAG CCTCCCCATTGAAAAAGATCATAAAAATAGATGCGAGTATCTTTCTTAGCACCAAGAGATAGGGTGATAG GGTTATTGTTGGTTGTTCCAGGAATATAAAAAAGTTTTCGCAAACCTGCTGACATTCCGGTAGTTGAAAG CTCTGTATTATTGTTGAAGATAATATCCCCAGAGCCAGTATGAATATAGTTCGCAGCTGGGCTTGCAAAT AGGTGAAAAGCTCCTCCATACAGAGCCGAATTATTAAGAAAATAGATAGGACCATTTTCTTGAAAATTAA GGTTGTTGCAGGAAATAGCTCCTGCTATGTATGAGGCATTATTATTCTCAAAGTAGATAGGGCCTTTATT ATTTAGAAGACCAAACTCTCCTCCTGCGCTTATAACTGCTCCATTTCCTTGAGCACTAGTGTTATGCCAT CCTAAAGCGCTATTATTTATAAAAATAAGCTTATCCTTATTATCTCTAAAAATACACTGTCCCCCGGTGC TGATAGCTCCTCCGTATCCTCCATAGTTGGAATTGAAGACTACCGGACCAGTATTGTGTGAGATTTCGCA GGCACCTTGGCAGCCAACAGCTCCTCCTCGAGCAAGAATTGTAGCAAATGTGTTGGGTATGTGATCAGTA GTATGGTTATTTTCAAAAGCAACTATTCCGGACGTATTTTTGATAACTAGGTTTTGGATTGTGGATACGG CTCCTCCCATATCCCCAGAAGTGTTATTGATAAAATTTAAGCTATGATTATTAATTAGCTCGATGCTTGA TCCGTATAAAGCCCCTCCGCGATTCCTTTCGGTTCTTAATGCTACTCCTGTAAATTGAGTTCCAATAGTT GTATTATTATAGAACAATATGTTTTGGTTGTTTTTAAAGCTAATAAGGCCTGTAGCATTTATAGCTCCCC CTCCTCCCTGATGGGTGCAATAATTGTTACAAAAAAACAAGTCTGCATTGTCAATGGCTGTGAAAGAAGT CATAGACTCAACAAAAGCTCCACTAGAGTTTGTAGTGCTTAAACCTCTCGAAAACCATTGATCCTGCGTA CAACTTGAAATAGTAAAGTTCCCTCTATAATTAGAAACATTTGCGTAAGTGATGTATTGAGCAGCAATGG AGCTAGGCGGAGTAACTATGAATTCAATCTCTCTACCACCAAAAGTCAAGTTCGGAAGTTTGAGTGTATC GGTTTCACTGGCCGAAATACAAGATAAAGGAATGAAAACAAGTGAAAGAGGGAGAATTCTGCGAGTCA >WP_010229975.1 autotransporter domain-containing protein [Chlamydia muridarum] MTRRILPLSLVFIPLSCISASETDTLKLPNLTFGGREIEFIVTPPSSIAAQYITYANVSNYRGNFTISSC TQDQWFSRGLSTTNSSGAFVESMTSFTAIDNADLFFCNNYCTHQGGGGAINATGLISFKNNQNILFYNNT TIGTQFTGVALRTERNRGGALYGSSIELINNHSLNFINNTSGDMGGAVSTIQNLVIKNTSGIVAFENNHT TDHIPNTFATILARGGAVGCQGACEISHNTGPVVFNSNYGGYGGAISTGGQCIFRDNKDKLIFINNSALG WHNTSAQGNGAVISAGGEFGLLNNKGPIYFENNNASYIAGAISCNNLNFQENGPIYFLNNSALYGGAFHL FASPAANYIHTGSGDIIFNNNTELSTTGMSAGLRKLFYIPGTTNNNPITLSLGAKKDTRIYFYDLFQWGG LKKANTPPENSPHTVTINPSDEFSGAVVFSYKNISSDLQAHMIASKTHNQIKDSPTTLKFGTMSIENGAE FEFFNGPLTQESTSLLALGQDSILTVGKDASLTITHLGIILPGLLNDQGTTAPRIRVNPQDMTQNTNSNQ APVSTENVATQKIFFSGLVSLVDENYESVYDSCDLSRGKANQPILHIETTNDAQLSNDWKNTLNTSLYSL PHYGYQGLWTSNWMTTTRTVSLTNSTETQTANNSIQEQKNTSETFDSNSTTTAKIPSIRASTGGTTPLAT TDVTVTRHSLVVSWTPIGYIADPARRGDLIANNLVSSGRNTTLYLRSLLPDDSWFALQGSAATLFTKQQK RLDYHGYSSASKGYAISSQASGAHGHKFLFSFSQSSDTMKEKRTNNKISSRYYLSALCFEQPMFDRIALI GAAAYNYGTHKTYNFYGTKKFSKGNFHSTTLGGSLRCELRDSMPFQSIMLTPFIQALISRTEPASIQEQG DLARLFSLKQPHTAVVSPIGIKGVYSSNKWPTVSCEMEVAYQPTLYWKRPILNTVLIKNNGSWETTNTPL AKHSFYGRGSSSLKFSYLKLFANYQAQVATSTVSHYMNAGGALVF Protective antigen Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. In addition, pmpE/F-2 was able to engender protective immunity against challenge with C. muridarum [Ref1062:Yu et al., 2009]. PmpG-1 Chlamydia muridarum str. Nigg VO_0010891 1246433 497915821 TC_RS01335 AE002160 WP_010229977 243161 311528 314488 + Pmp family polymorphic membrane protein autotransporter adhesin 5.8 98285.26 986 similar to GP:4376736; identified by sequence similarity; putative >NC_002620.2:311528-314488 Chlamydia muridarum Nigg, complete genome GATGCAAACGCCTTTTCATAAGTTCTTTCTTCTAGCAATGCTATCTTACTCTTTATTGCAAGGAGGGCAT GCGGCAGATATTTCCATGCCTCCGGGAATTTATGATGGGACAACATTGACGGCGCCATTTCCCTACACTG TGATCGGAGATCCCAGAGGGACAAAGGTTACTTCATCGGGATCGCTAGAGTTGAAAAACCTGGACAATTC CATTGCGACTTTACCTCTAAGTTGTTTTGGTAATTTGTTGGGGAATTTCACTATTGCAGGAAGAGGGCAT TCGTTAGTATTTGAGAATATACGAACATCTACAAATGGGGCGGCATTGAGTAATCATGCTCCTTCTGGAC TGTTTGTAATTGAAGCTTTTGATGAACTCTCTCTTTTGAATTGTAATTCATTGGTATCTGTAGTTCCTCA AACAGGGGGTACGACTACTTCTGTTCCTTCTAATGGGACGATCTATTCTAGAACAGATCTTGTTCTAAGA GATATCAAGAAGGTTTCTTTCTATAGTAACTTAGTTTCTGGAGATGGGGGAGCTATAGATGCACAAAGTT TAATGGTTAACGGAATTGAAAAACTTTGTACCTTCCAAGAAAATGTAGCGCAGTCCGATGGGGGAGCGTG TCAGGTAACAAAGACCTTCTCTGCTGTGGGCAATAAGGTTCCTTTGTCTTTTTTAGGCAATGTTGCTGGT AATAAGGGGGGAGGAGTTGCTGCTGTCAAAGATGGTCAGGGGGCAGGAGGGGCGACTGATCTATCGGTTA ATTTTGCCAATAATACTGCTGTAGAATTTGAGGGAAATAGTGCTCGAATAGGTGGAGGGATCTACTCGGA CGGAAATATTTCCTTTTTAGGGAATGCAAAGACAGTTTTCCTAAGTAACGTAGCTTCGCCTATTTATGTT GACCCTGCTGCTGCAGGAGGACAGCCCCCTGCAGATAAAGATAACTATGGAGATGGAGGAGCCATCTTCT GCAAAAATGATACTAACATAGGTGAAGTCTCTTTCAAAGACGAGGGTGTTGTTTTCTTTAGTAAAAATAT TGCCGCAGGAAAGGGGGGCGCTATTTATGCTAAGAAACTGACAATTTCTGACTGTGGTCCGGTCCAGTTT CTTGGTAATGTCGCGAATGACGGGGGCGCTATTTATCTAGTAGATCAGGGGGAACTTAGTCTATCTGCTG ATCGCGGAGATATTATTTTTGATGGAAATTTAAAGAGAATGGCTACGCAAGGCGCTGCCACCGTCCATGA TGTAATGGTTGCATCGAATGCTATCTCTATGGCTACAGGGGGGCAAATCACAACATTAAGGGCTAAGGAA GGTCGCCGAATTCTTTTTAATGACCCTATTGAAATGGCGAATGGACAACCTGTAATACAAACTCTTACAG TAAACGAGGGCGAAGGATATACGGGGGACATTGTTTTTGCTAAAGGTGATAATGTTTTGTACTCAAGTAT TGAGCTGAGTCAGGGAAGAATTATTCTCCGAGAGCAAACAAAATTATTGGTTAACTCCCTGACTCAGACT GGAGGGAGTGTACATATGGAAGGGGGGAGTACACTAGACTTTGCAGTAACAACGCCACCAGCTGCTAATT CGATGGCTCTTACTAATGTACACTTCTCCTTAGCTTCTTTACTAAAAAATAATGGGGTTACAAATCCTCC AACGAATCCTCCAGTACAGGTTTCTAGTCCAGCTGTAATTGGTAATACAGCTGCTGGTACTGTTACGATT TCTGGTCCGATCTTTTTTGAAGATTTAGATGAAACTGCTTACGATAATAATCAGTGGTTAGGTGCGGATC AAACTATTGATGTGCTGCAGTTGCATTTAGGAGCGAATCCTCCGGCTAACGCTCCAACTGATTTGACTTT AGGGAACGAAAGTTCTAAATATGGGTATCAAGGAAGTTGGACACTTCAATGGGAACCAGATCCTGCGAAT CCTCCACAGAACAATAGCTACATGTTGAAGGCAAGCTGGACTAAAACAGGTTATAATCCTGGTCCGGAGC GCGTAGCTTCTCTGGTCTCTAATAGTCTTTGGGGATCCATTTTAGATGTGCGTTCCGCGCATTCTGCGAT TCAAGCAAGTATAGATGGACGAGCTTATTGTCGGGGTATTTGGATTTCTGGGATTTCGAACTTTTTCTAT CATGATCAGGATGCTTTAGGACAGGGGTATCGTCATATTAGTGGGGGATATTCGATAGGAGCAAACTCTT ATTTCGGGTCTTCTATGTTTGGACTTGCTTTTACTGAAACTTTTGGTAGGTCCAAAGATTATGTGGTCTG TCGATCTAACGATCACACTTGTGTAGGCTCTGTTTACTTATCCACTAGACAAGCGTTATGCGGATCCTGT TTATTTGGAGATGCTTTTGTTCGGGCGAGTTACGGATTTGGAAATCAGCATATGAAGACCTCTTATACAT TTGCTGAAGAGAGTAATGTGCGTTGGGATAATAACTGTGTAGTGGGAGAAGTTGGAGCTGGGCTCCCTAT CATGCTCGCTGCATCTAAGCTTTATCTAAATGAGTTGCGTCCCTTCGTGCAAGCAGAGTTTGCTTATGCA GAGCATGAATCTTTTACAGAGAGAGGGGATCAGGCTAGGGAGTTTAAGAGTGGGCATCTTATGAATCTAT CTATTCCAGTTGGGGTGAAGTTTGATCGATGCTCTAGTAAACATCCTAACAAGTATAGTTTTATGGGAGC TTATATCTGTGATGCTTACCGGTCCATTTCTGGAACGGAGACAACACTCCTGTCTCATAAAGAGACTTGG ACAACAGATGCTTTCCATTTAGCAAGGCATGGAGTTATGGTCAGAGGATCTATGTATGCTTCTTTAACAG GTAATATAGAAGTCTATGGCCATGGAAAATATGAATACAGGGATGCCTCTCGAGGGTATGGTTTAAGTAT TGGAAGTAAAATCCGATTCTA >WP_010229977.1 autotransporter domain-containing protein [Chlamydia muridarum] MQTPFHKFFLLAMLSYSLLQGGHAADISMPPGIYDGTTLTAPFPYTVIGDPRGTKVTSSGSLELKNLDNS IATLPLSCFGNLLGNFTIAGRGHSLVFENIRTSTNGAALSNHAPSGLFVIEAFDELSLLNCNSLVSVVPQ TGGTTTSVPSNGTIYSRTDLVLRDIKKVSFYSNLVSGDGGAIDAQSLMVNGIEKLCTFQENVAQSDGGAC QVTKTFSAVGNKVPLSFLGNVAGNKGGGVAAVKDGQGAGGATDLSVNFANNTAVEFEGNSARIGGGIYSD GNISFLGNAKTVFLSNVASPIYVDPAAAGGQPPADKDNYGDGGAIFCKNDTNIGEVSFKDEGVVFFSKNI AAGKGGAIYAKKLTISDCGPVQFLGNVANDGGAIYLVDQGELSLSADRGDIIFDGNLKRMATQGAATVHD VMVASNAISMATGGQITTLRAKEGRRILFNDPIEMANGQPVIQTLTVNEGEGYTGDIVFAKGDNVLYSSI ELSQGRIILREQTKLLVNSLTQTGGSVHMEGGSTLDFAVTTPPAANSMALTNVHFSLASLLKNNGVTNPP TNPPVQVSSPAVIGNTAAGTVTISGPIFFEDLDETAYDNNQWLGADQTIDVLQLHLGANPPANAPTDLTL GNESSKYGYQGSWTLQWEPDPANPPQNNSYMLKASWTKTGYNPGPERVASLVSNSLWGSILDVRSAHSAI QASIDGRAYCRGIWISGISNFFYHDQDALGQGYRHISGGYSIGANSYFGSSMFGLAFTETFGRSKDYVVC RSNDHTCVGSVYLSTRQALCGSCLFGDAFVRASYGFGNQHMKTSYTFAEESNVRWDNNCVVGEVGAGLPI MLAASKLYLNELRPFVQAEFAYAEHESFTERGDQAREFKSGHLMNLSIPVGVKFDRCSSKHPNKYSFMGA YICDAYRSISGTETTLLSHKETWTTDAFHLARHGVMVRGSMYASLTGNIEVYGHGKYEYRDASRGYGLSI GSKIRF Protective antigen Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. Overall, vaccination with DCs transfected with PmpG-1(25-500) exhibited the greatest degree of protective immunity among the four Chlamydia Ags tested [Ref1062:Yu et al., 2009]. RplF Chlamydia muridarum str. Nigg VO_0010892 1246168 497917443 TC_RS04070 AE002160 WP_010231599 243161 941594 942145 - 50S ribosomal protein L6 10.64 18060.75 183 ribosomal protein L6 appears to have arisen as a result of an ancient gene duplication as based on structural comparison of the Bacillus stearothermophilus protein; RNA-binding appears to be in the C-terminal domain; mutations in the L6 gene confer resistance to aminoglycoside antibiotics such as gentamicin and these occur in truncations of the C-terminal domain; it has been localized to a region between the base of the L7/L12 stalk and the central protuberance >NC_002620.2:941594-942145 Chlamydia muridarum Nigg, complete genome TCTATTTTTTACCAGTTTTCGCTGCTTTTCCAGCCTTACGACGTACATATTCGTTTTCGTAACGAATTCC TTTACCTTTGTATGGCTCTGGAGGGCGTTTTGCACGAACACAAGCCGCAAATTCTCCTACCAACTGCTTG TTGATACCTTTGATGGAGATCAATGTGTTCTTCTCAACAGAGACTTCCAATCCTGTAGGAATAGGCATTT TTGTTGGATGGGAAACCCCTATCGATAAATCTAAGAAGGACCCCTGTACCGAAGCTCTGAATCCAACTCC GATCATTTCTAAACGTTTTTCAAATCCAAGGTGGACACCTTTGACCATATTTGCTATCAAAGCCCAATAA AGCCCTTGCATACGACCAGGTCTGTCTATAATGTGAGCTGCGGGAGAAACAAACACCTCATTACCTTTAA CGGCAATCTCAACTTCTTTAGCCAACACCTGTGTCAAAGACCCTTTAGGACCTTTTACTGAGATTTCATC ATTTTGAATAGAGACCTCTACGCCTTGAGGAAGTACAATAGGGTCTCGAGCTTTACGAGACA >WP_010231599.1 50S ribosomal protein L6 [Chlamydia muridarum] MSRKARDPIVLPQGVEVSIQNDEISVKGPKGSLTQVLAKEVEIAVKGNEVFVSPAAHIIDRPGRMQGLYW ALIANMVKGVHLGFEKRLEMIGVGFRASVQGSFLDLSIGVSHPTKMPIPTGLEVSVEKNTLISIKGINKQ LVGEFAACVRAKRPPEPYKGKGIRYENEYVRRKAGKAAKTGKK Protective antigen Mice vaccinated with DCs transfected with individual Chlamydia protein PmpG-1(25-500), RplF, or PmpE/F-2(25-575) exhibited significant resistance to challenge infection. In addition, RplF was able to engender protective immunity against challenge with C. muridarum [Ref1062:Yu et al., 2009]. Tarp Chlamydia muridarum str. Nigg 1245826 497916366 TC_RS02355 AE002160 WP_010230522 243161 571045 572607 + peptide ABC transporter substrate-binding protein 9.01 56833.03 520 similar to GB:D00760, SP:P25787, and PID:220024; identified by sequence similarity; putative >NC_002620.2:571045-572607 Chlamydia muridarum Nigg, complete genome CATGCGCAAGGTATCAGTGGGAATCTGCTTGCTTATCGCATTAGCAACAGCAATTACCGGCTGCTCCAAA TCCTCCTCAAACAAATCAAATCACTCCTCCTCTAATCAAAGCGTATCTGTGAGCATGAAGGACGATCCTC GCACGTTTGACCCTCGAGAGGTTAGGCTTCTTTCGGATATTAATTTGATTCACCATCTTTATGAGGGACT GGTTCAAGAGACTCCTTCCGGAGAGGTTTTCCCTGCATTAGCAGAAAGTTTCTTCCTATCTGAAGATAAG AAAACCTACACTTTCCACCTAAAAAAAGCACTCTGGAGTAATGGAGACCTGATTACTGCTCATGATTTTG TACGTTCTTGGAACGATGTTTTGCAAAATCGCATTGCAAGTATCTACTCTTTCGCTTTTTTACCCATTGA TTTAAGTAAAGATTGTGGATTCTTCGCTAAGGACAATCATACACTTGTTATCAATTTGCATACCCCTACT CCACACTTTCTGAAATTACTTACCCTTCCAGTATTTTATCCTGTGCACCCCGAACATCAAATTCGGAATG AAGCAAAAGCTCTTCCAATATCTACAGGGGCTTTTTGTTTAAAAGAGAAAAAAGATCGGCGGTGGCTTAA ATTAGAAAAAAATCCCTATTATTATAACAAAGAGCAAGTTGCGATCCAGGAAATCCACATACATGTGATT CCTGATCAGCAGACCGCTTCTGCTTTGTTCAAACAAGGAAAATTGGATTGGCAAGGACTTCCTTGGGGAC ACAGCATTCCCCAAGAAGCTTTAGCAACAGCAAATAAACGTCGTACTCCTCAATCATTTGATATATCTGG CACCTCGTGGCTCACGTTCAACACATCAAAAGTACCCTTCAGCCATCCCAAGTTGCGACAAGCTTTAAGC CTTGTTCTAAATAAGGAAGCCTTAGCCTCTCCAACCTTTGTTAAGCCCGCTAAACACCTACTTCCTACAC ACCTGCACACTTATCCAGAGCAACCTACTTACAAACAACAAGAAGCCGTCATTTTAGCCAAAACTCTGTT ACAAGAAGCATTAACAGATCTTAATATGACTATAAAAGATCTTGAAAAATGCCCTTTGATTTTTTCAGCG ACATCTAGCGTCAACTCACAAATGGCCCAAATGATGCGCGATCAATGGCGAAGAATTTTAGGAATAACAT TCCCTATTTGCGGGAAAGAGTATGCTTTATTACAAAATGATTTGACCACCAACACTTTTTTTATGTCCAT AAACGGTTGGTTTGCAGACTTTTCCGATCCTTTAGCCTTCTTGTCTGTTTTTTCTTCTAAGGGAATCAAA CCTTATGCCTTACAAGACCCTCTGTTTGACCAGCTTATTCTCTCTATAGAAACGGAAAAAGATCCTCGAA AGCGTATTTCTCTGATTTCCGAAGCTTCTCTATACCTAGAAAAACAAAATATCATAGAACCGCTCTATCA CGATGTTTTTCACTATGCCGCAAATAATAAACTTTCTTTTGTTAGATTACATCCCTCAGGTCTTGTCGAT ATGCGGTATGCAAAAAACTCTTG >WP_010230522.1 peptide ABC transporter substrate-binding protein [Chlamydia muridarum] MRKVSVGICLLIALATAITGCSKSSSNKSNHSSSNQSVSVSMKDDPRTFDPREVRLLSDINLIHHLYEGL VQETPSGEVFPALAESFFLSEDKKTYTFHLKKALWSNGDLITAHDFVRSWNDVLQNRIASIYSFAFLPID LSKDCGFFAKDNHTLVINLHTPTPHFLKLLTLPVFYPVHPEHQIRNEAKALPISTGAFCLKEKKDRRWLK LEKNPYYYNKEQVAIQEIHIHVIPDQQTASALFKQGKLDWQGLPWGHSIPQEALATANKRRTPQSFDISG TSWLTFNTSKVPFSHPKLRQALSLVLNKEALASPTFVKPAKHLLPTHLHTYPEQPTYKQQEAVILAKTLL QEALTDLNMTIKDLEKCPLIFSATSSVNSQMAQMMRDQWRRILGITFPICGKEYALLQNDLTTNTFFMSI NGWFADFSDPLAFLSVFSSKGIKPYALQDPLFDQLILSIETEKDPRKRISLISEASLYLEKQNIIEPLYH DVFHYAANNKLSFVRLHPSGLVDMRYAKNS Protective antigen Immunization of mice with Tarp induced Th1-dominant immunity that significantly reduced the shedding of live organisms from the lower genital tract and attenuated inflammatory pathologies in the fallopian tube tissues. Tarp, an immunodominant antigen identified by human antisera, can induce protective immunity against chlamydial infection and pathology in mice [Ref1623:Wang et al., 2009]. TC0439 adherence factor Chlamydia muridarum str. Nigg VO_0010887 1245792 497916297 TC_RS02190 AE002160 WP_010230453 243161 526719 536375 + adherence factor 7.34 344190.12 3218 similar to GP:6013469; identified by sequence similarity; putative >NC_002620.2:526719-536375 Chlamydia muridarum Nigg, complete genome TATGACTCTTTCGAATACAGCCTCTTCCACAGTTTCACCTAAGTCTACGCAACTTAACCATACTTTATCC AATCAAACTCACCCTGTTGCTCAAAGCAATAGGAATATAGATTTCCAAATCTATGATGAACAGATTCATA ATAATCATTCTACAGAAGATGTAGTTGCTATAGGACGTCGTATTCAGCAAGAATATGCGAATCTTACCAC ATCAAAACAGGTTAATTTCTCGGCATCTCCATCTAACCATACTGGAAACTGGAAGATTTCTTTGCTTTAT AATTTATCGATGCTGGTTGCAAATCTATTCCCTACAACTATTCAGCCTGTGCAACCTCAAAAAGTCATCT TTAAGAATCAAGACTCTTCTAAGGAAACCCAACTGACCAAATCATCCAAAGTAGTTTCTTCTCGCTCTTC TTTAAAAGAGATTTTTTCGAAGAAATCTCCTCCTTTGAGAAAGTCTTTTTCTTCCTCTAAAATTTCTAGA GCAATCCCTAGAAGAAAAAAACGATCAAACGATGATCCAGTTCAAGCCACAAATACTTATGATTTGACCG CTGAAAATATTCTTGAAAAACTCTCTTTAACACAAGAACAACAAATTAAGCACGATAATCTGATTAGCAA TCTCAAAGAAGCAATTAATCGATATAGTGATCTTAATCGGAAAAATTCCCGGAAAGGACAATCCCTTCTT GTTAGGCAAGCCAAAATTCTAGATGAAATTCTTTCACAGACTAAATCTACAGAGGAACGAGCTTCCAATT CTGTAATGACGACTATTAAGAAGGAATTTACCTCCCACCGCGTACCTGTAGAGAAAAATATTCACGGAAT TTGGATTGCAGGTTCACCGCCAGAAGGGACCGATGAATATATTAAATTGTTCCTTCACACTTATCCAGAG TTTTCCTTCCTTTTTTGGGTGGATAAAACAGCTTATGGCGCAGCAAAATTCTCTTCTACTCTTAAACGAA TTGCTTTTGATGCAGCAGTTAATTCCTTACGAGAGGCTACTCCAGAGCCTGTCAAGCAGTTTGTTCAACG TTATGATAAGCTAAAAAAATCATACGATACTTCGCGAGATTTTGATGAAAAACAGCGGTTATCTGAGCAG TTAGTTGAGCTATACGATAATTACAATAAATTTAGTAAGGAGATTCAAAGTAACTTTGATGTTTTGCTAT TGCATGAAATGATCACTATTCAAGATAGCTTTTTTAACTACTGCCAGCTCAAAGGAGTAGGTGCAATAAC AGATGAGACACGTATAGAGTATCTTGAGAAAGTTTTGAAAGTAAAAGAAGAGGATCTATCTCACTATAAA GAAACTATCAAACGCAACAAAGAGTCTATTGAAAAACTTGTAAAAGAGATTAATGACTCTACTGGACGGG AGAGAGTAGTTATCAAAGACATCCGAGATTTGAAGTCTTTACAAGATCTTACTAATAGCTACAACTATGA AACAGAAATGTTACTCCGATGGAATTATGCAGCGGCTACCGACCAGCTTCGTATGTACATGCTTAAAGAA TATGGGGGGATTTATACAGACCTAGATATTATGCCACAATACTCTCAGGATGTTTTGCAAAAAATTATGG ATGTTGGTGGGAGTAGGTTTTTTGAGCATGATAAGTTACGGCGGACTCTTTCTTTTGCAGCTCTAAAATT AGGAAGCGGTAAGCAAACAACTGTTTCCTTTGAAGAGGCTAAAAAAGCTATGACTCTCCCCACATTTACT CTTCAGGATAAGAGTCAAATTTCTGAAATTTTTAAATATCTAGAAACGGAAACGCAAGCAAAAAAATCAC TTTTCCAACCGATGGATGTTACCGTCGTCCGTGATTTCATGCCTATTTTACAACGTTATCATAAGTGGCA AACAGGATGGAATGTTCGGGGATTAAATGGTTTAATGATGGCTCATAAAGATAGTGCTGTTGTCGATGCT GTTATTGCTCGCCAGCGAGCGGCCTATGATGAGATGCGTGCACTTCGTCAGAATGTTGTTAGTGGAGAAT TTTTTCGTTCTCTTGGAGATCTTGAACATGTAAATCGTGAAAAGAATATCGGCGGCTATTTAGCGAAAAA TTATCTTGGGGGCAGTCTTTTTTTCGATTTTCGTCAAGATTCAGTGATTCCAGGAGCTATTAGTACCTTA GGAATTTCAGGTCCCGATATCATCATGGATACCATGAGCGATTATTTTACAAATCTTGGACCAGTTGGAG AAGATTTTTTATATGAAGGGAAATTAGGAAAAGCAGCTTTCCTTGGAGCTTATCAAGCACAAAAAACTCC GAAAGGAGAACTTACCTATGATTGGCTCCATCCTTTATCCATTGGAGCTAATGACGTTACTCCTGCTGAT GCAAGCACCTGGTGTGAAACTCGACAGCATTGTGCCGCTGAGCTTCTTTTATCGGACTCTATATCCTCAG ACGAACACCCTAAGGGTATTCGTCGAGAAAGAGTAAACCCGAATGATTTCTCTAAATTATGGAGTAAGGA AGCTCAGGGAATCCTTTCTAGTGATTTCGCGGATCTTTTACCACGCTTTAACTTACTGATAGAATCATCT GCGTTAGATATTCATACTTTATCGGCTCTTGATCGAGATATCCAACACCTTTTTACAAAAGTTCAAAAAG ACCCTGTAGCATCTGTAGCTGTATTTTCTTTACAGTTACAATTAGCTGAAATGATTCGTGCGATTCCTTT CCCTATACGTAATCAGGTACATATCTTGCCTGAAGCACAAGCACATTTCGAAGCAGATTGGAAGAAAGCA ATCCAATTATATCTTCACAGTCATCCCCAAACAGAAGTGGTTATTTGGTACAGTTCTACTCATACTCAAA TTGTTTTTGGAAAAGATTTGCTTGCTGTTGCAGAACGAGTTGCAGCAGCAAAATCCTTAATGTCTGATCA TGATCCATCACTTATAACCAGTTATCTAAAATATAAAACTCAATCTCATCTTGGGGTGCTTACTGAGTTT GATCAAGAAGATTTCTTTGAACTGATGGTAGATATTGCAGAGGAACCAGAGCTTCATAAACAACTATTGA AAATAGAAGAACAAGTTAATTCAGGACTCTACTCTCATGTAGAGCATTCTTTAGGAGAGTGGTTAAAATT ATCCAAAGAAGAAAGAAAATCAAAGTTTCTTAAGATTTTAAAAGAAACGTTTCAAGAGGAGGAAAGAGAA GACTCTCAACAACAACATAAAACATGGTTTGAAGAACTCTATGAGAAAAGACATCAGGAGCGCGTAAAGG ATCCAGCAAAAAAAATCCAAGAACTAATCACAGTTTTTCAAGAAAGTCAGCGCGTTCAAGCTCAAGACAT AGATACTTATTTTGCTCATAAACCGTTTTATCAAGATCTGATGAAAGATGGTTACGCTTTTGAGGACATT TCTGTGATTACGAAGTATCTTTTAGCGAGTGACGGTGTTTCAGGGATTATTACTACTGATCCTATATTTC CTCCTCCATCTAAACAGCTAATTGACGCTATGAAACAATCTTTGGGAGAAGATTTTGGTGAGCTACACTA TACGTTACAGATGGTCTATGATTGGTTATCTAAAGAGACTAACAGCGTAACTTCTGAACAGGCAAAACAA AAATTACCACAGAAACTACATGAAAAACTAGAAGGTTATACAACACATGATTTGCTTATTCCGCCTATTG ATGGAAGCGTCAGCGCTTTAGGATTGCGATTTTCCACGGAGGAGGGGAAGGTATCCGATCGCGTTTTAAC AAGCATAGCACCTGGAGTTCCTAATTCTGCTAGCTATGCTATGACTAGTTATTTGTATGGTTTGTTCTTA ATTACGAAAGATATCCAAAGTGGTCGATTAACCCATGAAATACTAAAAGAGAGATTACAAACCTATGGAG GCGCCTATTTTATCAATGAATCAAAAATTGATGTGCTACTTGCTTTATCCCGGAAAAAAGCTCAGATATC CCTAATAGACGCCCATAAGGCTCTTACAGGTTTTTCTTCTTTTAGCGAAGCATCTCTGGCTTTACTTACA GGAAGGATGCCCGGAACAAGCAGAGTTCTATCAAGAGAAGTAGAGTTTGGACGACCTTCAGCTATCGTTA TGGAAGGAGCAACAGCTATTCGTGCTCAGAGTTACGATGCTGTTGGATTAAGGAAAGATTTTTTCTTGTT ACCTCATACAGTTCCTTCCATACAGTCTATTGTTGAGCAAGCGAAATATACAGTATTATCCTGGCCAGAA TTTTATGAAAATCATGCGGATAAATGGAACGATCTTGCTAATCGTTTTGGAGCTGAGGACCTTAGTGTTC ATCCGCAAACATTTTTATATGACACAGAAGGTCGCTGTATGGGGCTTGCTTTACTGTATATGTTAGCAGA TGACTCTGTATCCTACAGATTATTACAACAAAATCTGATGACTTTAGCTTCGTTATTTGATGAGCAAAAC AGAAGAAATATCCCTTTAACTCCAGCTGATCAGAAATTTTTAAATAAGGGGCTTTCTTTAATTGAGTGGT TACAGTTCAAAGGAAATCAACAACTTCAAACAGAGGGATTTTTCCATACCTTAGACTGGGATATCCCTCA ATTAATGAAGCACTTTGCTTCTTCAACTGTAAAAAGTTGGTTAATTACTACGCCTGCGCATAGTCTTGTT CTTTCTTTAATGGGGAATTTTTTTAGAGTAACAGATCCAAACTATGGCCATACAGACTTTCCTTCATTAG AAGCTGCTATTACTTTTTTAGAAAGGATGGTTCAAGTATCCCCAGCTGTTTTGGAGCGTTATGGATTTGA TAAGGAAAAGTCTGTAACTAGCCAACTCAAAGTCCACTCTTTAGAGACTTCAGAATTGCAAAATGCAGTT TTTGCTTCCTCAGATCTTGGCTTTACTAGTCGCTATTTTACGACAACGTTAGAAGAGATGACCGTCAGAG GCCCTATTACGATGAATCAACGTCACACGGATTGGGCCACTCTTTATAAGATTGGGGGGACTGTTCAAGG TAAACGTATTGATAGTAGAACACGGGAATCGGATTTAAATTTTTTAAAAATTAATGGGGATATTCTAGAG GAATTTTTAACAAGAACTGTTCTAGATAGTGACTTGGTGGAGTTAATACAATCTCTGCTCAAAACACATG GCCTTGAGCCAGGCACTACCCTTATTAGTCCAAGTTCTATTGTAGAGACCGCAATAGACCACGTCTCGCT ATTACAAGCGGTGAAAACAAAAACCTCTCGAATGCACACTATTTTACAAAGCCTAGGAGAAAGAATTTTT AAATTGTTTAAAAATTCTGGGGTACAAGACTCAGACAAAATTTCTATAGACAGAGTCCAACTAGTTGATG AATCTGATAGCGCAACGATAGACTTCACAGTGATTAAAGATAAACAGCGTTCTCAGAAAAAAAGTATTAC TGTTGGCATAGAAAGTTTAGCTGGATCCTTTAGAAAATTTAGCGCATCGATGCACGAAGTTATTGGAACT GGAGTGCTTGATTTAGACTTAGGAATGACTGTCGTCTCTTTAGTTCAGTATGTCCGTTTAGTAGAAGCAG GACAAGGAAAAGATGCTTTAGCAGTTGCTAATTTAGTGATGAATCTTAAAATAGCTCTCGAGGTCTCTAT AGGGAATGTAATTCAGGCTCTGGGTAAAAAACTGCTCACTCAGGAGGGGCTTAATACTTTCCGTTTAGAA ACAGAATTAGCTCGTCAACTACACAAAGTGGGAGCACGAGTTGGAGGAACTGTTGGTAAGACGTTAACCA GAGTAGCGCATGTATTAGAACTCCCCATATTAGAAAGTGCAATTGGAACATGGAACCTCTATAATAGTGT GAACGAGTTATTACATGCTGATTCTTGGAGTGACCAGGTTGCTGCTAGAGTACAAGTAGCTTTTGATTCA ATCTCTTTAGGAATCACTATAGCTTCGGTTGTTTCTCCAGCACTTATGATGGCAGCAGGCCCCATTGCTG CCATAGGGATGGGGGCAGCATCCATTGCTCGTAATGTTGCCAGAAAAGAAGAGCGGCACTCTCAATGGCT CAAGTACAAGAGTTTTCTTGATAATGGATCAAAACATACAGTTGCCGCATTCCCTCATAAAGGGCTTATA GATTTATCTGAGAATTTAGTACTTGGGAACATTGTGCTGAATTTAAGGGTTTACCCACCTCTGCTTACAG GCGATCGCTCTTATAATGCAAATCGGTGGTTTGGGAATAAGCCTGGATGGTCTGATTGGCAAGTTCGAGA AAGATTAGGTTATGCCTATCGAAGCAGTCCATCTTATGCGTTAGCCAGAGGACACGCTAATAGTTTTTGG CCACTATCTATGCCTAGTATTGATAAAGGTGTATATCGCACAGTAATTCTAGGATATGGCATTCAATATA AAGCGGTTACAGAAGTTGTCTACTTGTCTAATGAAATGGTTTGGAGAGAAGCTGTGATGCAGTTTGACTC TCGATACTATGTAGAGCCATTAACAGCGGAAAAGGAGTGTGCAACAGTACTAGCAGGAGATACGCCACTA TCAGTGATTCCTGTACGTTTATTAGAAGAGGAGTCTCTGGAACGCGAGAAGAACGCGGCTTCTTATAAGA ACTATCAGATCATTATTGAGGGAGGGAAAGGAGGACTTACTGTACAGATAGGAGGAGCAGGTTATTACAA GTTAACAGCACAGCCAGGAAAAGGGAATATCCTCTCTTTCCGAGCAATACCTGGATATCTTTCTGTAACG TTTGATCTTAGCCGATTGGAACAAGAGGTGCCTCTCACCAAACAAAATGGAACAGCCTTAAAGATACTTA AAGTACGTCAAACTGGGTTTGATACTATTGTTGGATCTTCAGGAGGTAGTGATAATTTAACAGGCAATCA CAATACAAAATTTTATCTAAGCACCGGAGGAGGACATGTCACCTCAGGATCCGGTAAGAACTGGTATAAC ATTCCATCTTTAACACGTAGCCTTGGTTTTGATTTTACTTCTAATGCTATAGAGCATAAGGCTTCTGTAG AGATGCTTTTAGCAGATTTCTCTCCTGCTAATAATTTAAGTTTAATAGTTGAACGATTTGCAAATGTTAG CATTCATGTTTTTAATTATAGCAATAAAACAGCCCCTTATACTGTGAATTTGAAAGACGGGGTGTCTTTA CAAGCTTCTAAGCAAGGCTTTAGCGGTAGTTTATTAGAGGTGGCTTCCTTTGATCAAACGCTATGGCAAA AGAGCTATCCAGAAGATAGAGGATTTGTAGAAGATATTTTATCTTGGTTATTAAAACTGCAATGGTCGTT AGCTTCTCGAGTAAGGATCTTTTTACAGGGAGGCACAGCACAATACGAAACAACGCAGAAAAGTCTTATT TATAGACCTGATCCACACTCAAGTATATCTATTCAGGCTACAGATTCTTATAATACACAAGTTTATGGTA ACGTTGGCTGTTCTTATATTCTATTCTCTTCACCAGGTGTGACCGCCAAAACACTCGATATTTTGTTATT CGAAGATTCGGGGTTACCACAAATTATTGACTTAAGCACACTTGTTCCAACTTCTATCCAAGGGTTCCTC TATCCAGGAGGGTTTATAAACTTTCAAGTCTCTTCTGCAAGATATGCATTTCCTTTTTCTGTCAGTTGGA GAAGGCATCATTACACTTTCCCTGCTCAAACGATTATTCAAGTTCTACCTCGCTTGCGGTTTGAACTAGG GGATTGGTTTACAATTTTGCAAAAGAGTGTAGGAAAATGGGTAACATTGTACCAACACGACATGATTATC CCTGAACGTATTGAAGGTGTTTTAAGTCTAAATAACACAGCGACATTAATGTCACACCACAATCAAACAA CCCATATGCTTGGAGTAGAAAACAGGGGAGATTTGAGTCTAAAAGTTTTAGGAATACTCCAATCTGGTCA AATTAAAGGATCTAAATCGGAAAATAGCGCGAGTGGTCATTTTTCTAAATTGTTATCTGTCCCAGCACAT ACGATCAAAAATCTTTCGTTTAAAGAGGAAGAGGGAACTAAGAGTAAAAATATTTTATTCTACAGTGTGC TGGAGGAAACATTCCTTAAAGCTACAGATAAACCTTTAACTATTATTAAACGTGATAAATGGTCTCTGTA TGATGAAATCCAAGTATTTGCAACAACTCTTAATCTGCAAAATTTCTTGCGTTATCATATTTCGGAAGAG ACTCCAATGTTATCTAGACTGCTCATGTATGCGCAGAAACGCGTTTCCATCCAAAATAGAGATCTCGCAT TGAAATTTTTCTATGTTCGAGAGCGAAGTGGTATTGGTGCAATTAGATTGGTATTCAAGAACTTTTTCGA AGAGAGCTTGCAGGGGATATTACATGGAACCTTAGAGCGAGAAGTTAAACCTATGCTAGCTGAAAATCCA AATACACTAATTCATTCATCGTATCGGAATCATTTAGAACTCATCCTAGGGGAGGAAACCTTAGATCTCG CAATTATCGTTCAAGAGTTTAGCTCTTCAAAAAATATTGTTGAGTTGCAAAAAGATCTAGCAACCCATCG GCTTATTTATCCTCAAGGAAAAGAAGCCTTGTCTCTTGCAATCTATACCCATACTTTCAGTAAAGAGAGC TTAGTCACATCTAAACAACGAAGCGAAGGGTTAAAATTCATCGATCCATCAATGCAGGAATATCGTTTAC CTGAGACAACGATTCTCGAAAACTCTTATTATTTAGACCCTTCCACAGGAGATTTGTATTTAACAAAGAT AATTGCGCAACCTTCACAAAGTAGAGCATTTCTTATCAAATTTAAAAAATACAAACGCCACTGGCTAGAT TTCCAAAAATTAGTTCTTTCTGGATCTCATTTAGAACTTATCGCATCTACTGGAACAGCTTTAACATTTG TAGGCCCCGAATTACGGCATTTAGAGATTGACTTCCCAGGCGCGTTAAATGGGACTATTGCCAAAGAACA CATCTCATCTCGGGCAAGTATTGTAATCCCAACAAACAATCAGGTGACACACTATGACCCAAGAATAGCG AAGCAGCATTATAGCTCTATCAATTATATGCTGTGGGATCTGCGTGATCGTGCGACACTATCTAAGCGAG CAAAAACTTTAGATAGCTATTTGTTAGAAGTTTGTATGAACTTAGACTCTTCTAATCCAACGTGGAATAT TCCAAATGATGTGTTGAACTACGCGGTAGGATATTATCGAACAATCCTTCCTAGCTGGGTAAAAAATAAA ATCCGTGTAGGTTCCTTAATTAAAGTAGAAGCTGATACTGCAAAAACTTTGAGTTTCTCACTAATTACGA CACAAAATGATCTATTCCAACCTGTAGTGGATAAAGGATTCTATATTTACTACAGCGTTTCGAAACTAGC CAATCATGTTAAACTGCGAAATGTTACTGGAGATATGTTGCTTGATATCGATAAGGAAACCACATTTATT GTTCGAGGAGTAGATGAATCTGATTATGATAAGAAACAAATCTATGTTGTTTTAGATCTGACAACAGAAG AAGAACGTAAATTGCGAACGGATAAGAACATCATTATTATTCCTGGAGGAGAACGATTGAACCGATG >WP_010230453.1 LifA/Efa1-related large cytotoxin [Chlamydia muridarum] MTLSNTASSTVSPKSTQLNHTLSNQTHPVAQSNRNIDFQIYDEQIHNNHSTEDVVAIGRRIQQEYANLTT SKQVNFSASPSNHTGNWKISLLYNLSMLVANLFPTTIQPVQPQKVIFKNQDSSKETQLTKSSKVVSSRSS LKEIFSKKSPPLRKSFSSSKISRAIPRRKKRSNDDPVQATNTYDLTAENILEKLSLTQEQQIKHDNLISN LKEAINRYSDLNRKNSRKGQSLLVRQAKILDEILSQTKSTEERASNSVMTTIKKEFTSHRVPVEKNIHGI WIAGSPPEGTDEYIKLFLHTYPEFSFLFWVDKTAYGAAKFSSTLKRIAFDAAVNSLREATPEPVKQFVQR YDKLKKSYDTSRDFDEKQRLSEQLVELYDNYNKFSKEIQSNFDVLLLHEMITIQDSFFNYCQLKGVGAIT DETRIEYLEKVLKVKEEDLSHYKETIKRNKESIEKLVKEINDSTGRERVVIKDIRDLKSLQDLTNSYNYE TEMLLRWNYAAATDQLRMYMLKEYGGIYTDLDIMPQYSQDVLQKIMDVGGSRFFEHDKLRRTLSFAALKL GSGKQTTVSFEEAKKAMTLPTFTLQDKSQISEIFKYLETETQAKKSLFQPMDVTVVRDFMPILQRYHKWQ TGWNVRGLNGLMMAHKDSAVVDAVIARQRAAYDEMRALRQNVVSGEFFRSLGDLEHVNREKNIGGYLAKN YLGGSLFFDFRQDSVIPGAISTLGISGPDIIMDTMSDYFTNLGPVGEDFLYEGKLGKAAFLGAYQAQKTP KGELTYDWLHPLSIGANDVTPADASTWCETRQHCAAELLLSDSISSDEHPKGIRRERVNPNDFSKLWSKE AQGILSSDFADLLPRFNLLIESSALDIHTLSALDRDIQHLFTKVQKDPVASVAVFSLQLQLAEMIRAIPF PIRNQVHILPEAQAHFEADWKKAIQLYLHSHPQTEVVIWYSSTHTQIVFGKDLLAVAERVAAAKSLMSDH DPSLITSYLKYKTQSHLGVLTEFDQEDFFELMVDIAEEPELHKQLLKIEEQVNSGLYSHVEHSLGEWLKL SKEERKSKFLKILKETFQEEEREDSQQQHKTWFEELYEKRHQERVKDPAKKIQELITVFQESQRVQAQDI DTYFAHKPFYQDLMKDGYAFEDISVITKYLLASDGVSGIITTDPIFPPPSKQLIDAMKQSLGEDFGELHY TLQMVYDWLSKETNSVTSEQAKQKLPQKLHEKLEGYTTHDLLIPPIDGSVSALGLRFSTEEGKVSDRVLT SIAPGVPNSASYAMTSYLYGLFLITKDIQSGRLTHEILKERLQTYGGAYFINESKIDVLLALSRKKAQIS LIDAHKALTGFSSFSEASLALLTGRMPGTSRVLSREVEFGRPSAIVMEGATAIRAQSYDAVGLRKDFFLL PHTVPSIQSIVEQAKYTVLSWPEFYENHADKWNDLANRFGAEDLSVHPQTFLYDTEGRCMGLALLYMLAD DSVSYRLLQQNLMTLASLFDEQNRRNIPLTPADQKFLNKGLSLIEWLQFKGNQQLQTEGFFHTLDWDIPQ LMKHFASSTVKSWLITTPAHSLVLSLMGNFFRVTDPNYGHTDFPSLEAAITFLERMVQVSPAVLERYGFD KEKSVTSQLKVHSLETSELQNAVFASSDLGFTSRYFTTTLEEMTVRGPITMNQRHTDWATLYKIGGTVQG KRIDSRTRESDLNFLKINGDILEEFLTRTVLDSDLVELIQSLLKTHGLEPGTTLISPSSIVETAIDHVSL LQAVKTKTSRMHTILQSLGERIFKLFKNSGVQDSDKISIDRVQLVDESDSATIDFTVIKDKQRSQKKSIT VGIESLAGSFRKFSASMHEVIGTGVLDLDLGMTVVSLVQYVRLVEAGQGKDALAVANLVMNLKIALEVSI GNVIQALGKKLLTQEGLNTFRLETELARQLHKVGARVGGTVGKTLTRVAHVLELPILESAIGTWNLYNSV NELLHADSWSDQVAARVQVAFDSISLGITIASVVSPALMMAAGPIAAIGMGAASIARNVARKEERHSQWL KYKSFLDNGSKHTVAAFPHKGLIDLSENLVLGNIVLNLRVYPPLLTGDRSYNANRWFGNKPGWSDWQVRE RLGYAYRSSPSYALARGHANSFWPLSMPSIDKGVYRTVILGYGIQYKAVTEVVYLSNEMVWREAVMQFDS RYYVEPLTAEKECATVLAGDTPLSVIPVRLLEEESLEREKNAASYKNYQIIIEGGKGGLTVQIGGAGYYK LTAQPGKGNILSFRAIPGYLSVTFDLSRLEQEVPLTKQNGTALKILKVRQTGFDTIVGSSGGSDNLTGNH NTKFYLSTGGGHVTSGSGKNWYNIPSLTRSLGFDFTSNAIEHKASVEMLLADFSPANNLSLIVERFANVS IHVFNYSNKTAPYTVNLKDGVSLQASKQGFSGSLLEVASFDQTLWQKSYPEDRGFVEDILSWLLKLQWSL ASRVRIFLQGGTAQYETTQKSLIYRPDPHSSISIQATDSYNTQVYGNVGCSYILFSSPGVTAKTLDILLF EDSGLPQIIDLSTLVPTSIQGFLYPGGFINFQVSSARYAFPFSVSWRRHHYTFPAQTIIQVLPRLRFELG DWFTILQKSVGKWVTLYQHDMIIPERIEGVLSLNNTATLMSHHNQTTHMLGVENRGDLSLKVLGILQSGQ IKGSKSENSASGHFSKLLSVPAHTIKNLSFKEEEGTKSKNILFYSVLEETFLKATDKPLTIIKRDKWSLY DEIQVFATTLNLQNFLRYHISEETPMLSRLLMYAQKRVSIQNRDLALKFFYVRERSGIGAIRLVFKNFFE ESLQGILHGTLEREVKPMLAENPNTLIHSSYRNHLELILGEETLDLAIIVQEFSSSKNIVELQKDLATHR LIYPQGKEALSLAIYTHTFSKESLVTSKQRSEGLKFIDPSMQEYRLPETTILENSYYLDPSTGDLYLTKI IAQPSQSRAFLIKFKKYKRHWLDFQKLVLSGSHLELIASTGTALTFVGPELRHLEIDFPGALNGTIAKEH ISSRASIVIPTNNQVTHYDPRIAKQHYSSINYMLWDLRDRATLSKRAKTLDSYLLEVCMNLDSSNPTWNI PNDVLNYAVGYYRTILPSWVKNKIRVGSLIKVEADTAKTLSFSLITTQNDLFQPVVDKGFYIYYSVSKLA NHVKLRNVTGDMLLDIDKETTFIVRGVDESDYDKKQIYVVLDLTTEEERKLRTDKNIIIIPGGERLNR Protective antigen Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0439 showed significant levels of protection when compared to negative control animals [Ref1057:McNeilly et al., 2007]. TC0512 outer membrane protein, putative Chlamydia muridarum str. Nigg VO_0010885 1245872 497916494 TC_RS02600 AE002160 WP_010230650 243161 618235 620613 + outer membrane protein assembly factor BamA 9.51 84655.32 792 similar to GB:X75201, and PID:435005; identified by sequence similarity; putative >NC_002620.2:618235-620613 Chlamydia muridarum Nigg, complete genome AATGCTTGGAATACGAAAAAAAACGATTCTGCAACTCGCTATTTTACTGTTGCTCCCCTTTTCACAAAAC GCCTTTGGAACATCTCCTGAAGGACGTATGGTGGTACAATCCATCACTATTGAGACTCGAGGAGAAAATG CTCAAAATAAGCGGGCTATTCCCAAAATAAAGACAAAGCAAGGGACGCTGTTCTCTCAAACTGATTTTGA CGAAGATCTAAGAACTCTCTCGAAAGATTTCGACCGCATTGATCCTATTGTAGAGTTTCGCAATGGGCAA GCTGTTATTTCCCTTATTCTGACAGCAAAACCCGTTATCCGACACATTAACATCTCAGGGAATGAAGCAA TTCCTACTCATAAAATTTTAAAAACCCTAGAGCTTTATAAAAACGATCTTTTTGATCGAGAGGTTTTTTT CAAAAATTTTGATGCTTTAAGAACCCTTTACTTAAAACGCGGATACTACGATTCTCAATTGTCCTATTCT CACGATCATAATGAGCAAGAAGGTTATATTGATATCTCAATCCAAATTAAAGAAGGGCCCTACGGACGGA TAAAAAAATTAACAATTTCAGGTATTACTCGTACAGAAGCCTCTGATTTGGGAGACATAGTTTTAACCAA ACAGTACTCTACAACAACAAGTTGGTTCACTGGAGCAGGGGTATACCATCCAGACATGGTGGACCAGGAT CTCTTTGCCATAACAAACTATTTTCAAAATAAAGGTTATGCAGACGCAAAAGTCACAAAAGAAGTCTCCA CAGATGCTAAAGGAAACCTCTCTCTTGTTATCAACGTAGATAAAGGGCCCCTGTATACTTTAGGTCACGT ACATATAGAAGGTTTCACAGCTTTATCCAAGCGGTTACTTGATAAACAACTGTTAGTGGGCCCCAATTCC TTATATTGCCCAGAAAAAGTTTGGGCTGGAGCTCAAAAAATCCGTAATGCCTACGCTAGATACGGATATG TAAATACCAATGTAGACGTATCTTTTGCTGTTCATCCAACGCTCCCAGTTTATGATGTTACTTACAGGGT TAGCGAAGGAGCTCCTTACAAAATCGGACTAATCAAAATCAAAGGGAACACACACACCAAACATGACGTA ATTTTACATGAGACAAGTCTGTTCCCTGGAGATACTTTTGATAGATTAAAACTAGAAGATACGGAAACTC GATTACGTAACACAGGCTACTTTAAAAGTGTGAGTGTCTACACGGTACGCTCACAATTAGATCCTTTGGA CTCAAATAACCTTTATAGAGACGTTTTCATTGAAGTCAAAGAAACCGAAACAGGTAACCTAGGACTGTTC TTAGGATTCAGTTCTATAGACCATCTGTTCGGAGGAGCAGAAATTTCAGAAAGCAATTTTGATTTATTTG GCGCCCGACACTTATTCAAAAAAGGGTTTAAATCCTTAAGAGGTGGTGGGGAATATCTTTTCTTAAAAGC CAATTTAGGAGACAAAGTTACTGATTATACTGTTAAATGGACAAAACCTCATTTCTTAAACACCCCATGG ATCCTAGGAGTAGAATTAGATAAATCTATTAATAAGGCTTTATCAAAAGATTATTCCGTTGATACCTATG GAGGAAACATCAGCACGACATACATTCTTAACGACAAACTGAAATATGGGATGTATTACCGCGGGAGCCA AACGAGCCTAAGTTTAAGAAAAAAAACAGAAAATCCTAACAAACCTGGACCAGATCTAGATAGTAACAAG GGATTTGTTTCCGCGGCAGGGCTTAACGTTCTTTACGATTCTATTGACAATCCTAGAAAACCTACTATGG GTATTCGGAGCTCTTTGAACTTTGAGTTGTCTGGATTAGGAGGAACCTATCAGTTTACAAAATTAACAGC CAGCGGCTCTATCTATCGCCTGCTAACTAAAAAAGGTGTTTTGAAAATTCGTGGAGAAGCTAAGTTTATT AAACCTTTCGGTACAACCACAGCCCAAGGAATTCCTGTTAGTGAGCGCTTCTTCTTAGGAGGAGAATCTA CCGTTCGTGGATATAAACCATTTATTATTGGGCCTAAATTTTCTCCAACAGAGCCTCAAGGGGGCTTATC TTCCTTATTGCTAACAGAAGAGTTTCAATATCCTCTAATTTCTCAACCCAGCATCAATGCATTCGTTTTT CTAGATTCGGGCTTTATTGGCACGGAAGAGTACACTATTCGACTCAAAAATCTCTGTAGCAGTGCCGGAT TTGGGTTGCGTTTTGATATGATGAATAACGTGCCCATTATGTTAGGTTGGGGTTGGCCTTTCCGCCCAAC AGAAATTCTTAATAATGAAAAAATTGATGTATCTCAAAGATTCTTCTTCGCTTTGGGAGGAGTGTTCTA >WP_010230650.1 outer membrane protein assembly factor BamA [Chlamydia muridarum] MLGIRKKTILQLAILLLLPFSQNAFGTSPEGRMVVQSITIETRGENAQNKRAIPKIKTKQGTLFSQTDFD EDLRTLSKDFDRIDPIVEFRNGQAVISLILTAKPVIRHINISGNEAIPTHKILKTLELYKNDLFDREVFF KNFDALRTLYLKRGYYDSQLSYSHDHNEQEGYIDISIQIKEGPYGRIKKLTISGITRTEASDLGDIVLTK QYSTTTSWFTGAGVYHPDMVDQDLFAITNYFQNKGYADAKVTKEVSTDAKGNLSLVINVDKGPLYTLGHV HIEGFTALSKRLLDKQLLVGPNSLYCPEKVWAGAQKIRNAYARYGYVNTNVDVSFAVHPTLPVYDVTYRV SEGAPYKIGLIKIKGNTHTKHDVILHETSLFPGDTFDRLKLEDTETRLRNTGYFKSVSVYTVRSQLDPLD SNNLYRDVFIEVKETETGNLGLFLGFSSIDHLFGGAEISESNFDLFGARHLFKKGFKSLRGGGEYLFLKA NLGDKVTDYTVKWTKPHFLNTPWILGVELDKSINKALSKDYSVDTYGGNISTTYILNDKLKYGMYYRGSQ TSLSLRKKTENPNKPGPDLDSNKGFVSAAGLNVLYDSIDNPRKPTMGIRSSLNFELSGLGGTYQFTKLTA SGSIYRLLTKKGVLKIRGEAKFIKPFGTTTAQGIPVSERFFLGGESTVRGYKPFIIGPKFSPTEPQGGLS SLLLTEEFQYPLISQPSINAFVFLDSGFIGTEEYTIRLKNLCSSAGFGLRFDMMNNVPIMLGWGWPFRPT EILNNEKIDVSQRFFFALGGVF Protective antigen Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0512 showed significant levels of protection when compared to negative control animals. At 9 days following challenge TC0512 showed a 73% reduction in the number of recoverable Chlamydia compared with vector only immunized controls [Ref1057:McNeilly et al., 2007]. TC0757 hypothetical protein Chlamydia muridarum str. Nigg VO_0010886 1246122 497917305 TC_RS03850 AE002160 WP_010231461 243161 898853 899647 + hypothetical protein 5.02 27979.63 264 identified by match to TIGR protein family HMM TIGR01607 >NC_002620.2:898853-899647 Chlamydia muridarum Nigg, complete genome CATGCGCTGTTCTGCTTATTGCTCTGCTTCGTCCTATCATCTACACGTTCTTTTTCATCTTTTAAAGGCA CGCTACCCTTCTGTCTTATCCCGAGAATACGTGCTCATTTCCTCTGAAGAGCTGAATGAAAGCGACAAAG CCGCTGTCTTTTTCCCATTTGGAGTTAGTGTGTTTTGGGGATGGGAAGAAGCTGAAGAACTTCAAGTGAT CCAAGCTATCACCTCAGCATCAGTGGATCCTCTCCCTAACCCAGAAATTGATAGTTATGACTTCCATTAT GGAGAGAAACTACAGATCCGTCGCGATAGACTTGTTCTCACCAACTCTAATTTAAACACAAAACTTGCTA TTTCCTTTGGGTTAGCCCAATCTATCAAACTTACCGTCTTTGAAGAAACGATTTACAAAACAGTTGAAAA CTCTAAATCTTTGCCTCAAGAACTTGCCTCTAAAGGGAAAATTTCTCTATCCCGTAAAACGATCGCAAAA AAGATTGGAGAGCTTTTCCTGGATAAAGCTTCCGTAAACCTACACTCTGACATTCTCGATGAGCCCGACT TTTTCTGGGAACATCCAGAAACCCAACCTTTTTATATTGATGTGTTGACCTGCCTAGATGTAAACGCGCG AGTAAATGTATTAAATCACAGACTTGCTATCCTAGGAGATGTTCTCGAGATTTTAAATGACCAACTTAAT CATCAGCATTCTTCAGCTCTAGAGTGGACCGTGATCTGGTTAATTGCACTAGAGGTATTGGTCACCTTAC TTAAAGACGTATTCAACATCATCTA >WP_010231461.1 RMD1 family protein [Chlamydia muridarum] MRCSAYCSASSYHLHVLFHLLKARYPSVLSREYVLISSEELNESDKAAVFFPFGVSVFWGWEEAEELQVI QAITSASVDPLPNPEIDSYDFHYGEKLQIRRDRLVLTNSNLNTKLAISFGLAQSIKLTVFEETIYKTVEN SKSLPQELASKGKISLSRKTIAKKIGELFLDKASVNLHSDILDEPDFFWEHPETQPFYIDVLTCLDVNAR VNVLNHRLAILGDVLEILNDQLNHQHSSALEWTVIWLIALEVLVTLLKDVFNII Protective antigen Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0757 showed significant levels of protection when compared to negative control animals [Ref1057:McNeilly et al., 2007]. TC0767 hypothetical protein Chlamydia muridarum str. Nigg VO_0010888 1246132 497917334 TC_RS03895 AE002160 WP_010231490 243161 910619 911350 - polysaccharide deacetylase 9.14 26828.18 243 >NC_002620.2:910619-911350 Chlamydia muridarum Nigg, complete genome ATTAATGCAAAATAAGGAATCGTTTTAATTGGCGGAAAGAGAGCTGATAAAGAGATGGGATCCCTCGACT ATCTAAAGCCATATCAATACGAGGAATGCTATGCTGTGATTGCATCGAGTAAAAAAAACTCGCAGCATCC CCTAACACAAAAGAATAGCGGTAAACCTCCTGAACAAAATGTTGACTCACCAAATCACTTTTTCCAAAAG GATAAAAAAAGACTTCCACAGGAGCCTGTATAGCCTCTTCTAGTAAAATTTTAGACAATGAAATTTCAGT ATGTAAATAAGGAGGGGAGTTTTTAAGGTTTCTAATAGCAAACCCTGAAGAGGCAAATCGAATAATCGAA CTTTGAGCCATATGACAAAGTTCTTTAACCGAACAAAAGGGCTGATAATAAGAAAATATTTCGTCTTGAA AAGCTAGAAAATCCGATGGCGAAATTCGCATATCAATGGGAAGATTTTCACTTTCTAAACGGGATACGTA TCTCCAAGCAACTCCAACAACCGCAGGAATCTGGAATTTCTGAAGAAAAGGAAAGACATGCTTGTAAAAA TCAACAGAAGCATAATCGAATGTCAGTATGATTGCTTTCCTATTAGGGAGAGGATCTCCGGGCAGAATAA ACGAATAATGCTGCTTGAGAGAGTGCAAAAAATCTAAAAACGATCTGAGGGCATAAGGAAACTTAGAAAA AGCAACCTGTCGATAGGCTAAGACGCGGAGCA >WP_010231490.1 polysaccharide deacetylase family protein [Chlamydia muridarum] MLRVLAYRQVAFSKFPYALRSFLDFLHSLKQHYSFILPGDPLPNRKAIILTFDYASVDFYKHVFPFLQKF QIPAVVGVAWRYVSRLESENLPIDMRISPSDFLAFQDEIFSYYQPFCSVKELCHMAQSSIIRFASSGFAI RNLKNSPPYLHTEISLSKILLEEAIQAPVEVFFYPFGKSDLVSQHFVQEVYRYSFVLGDAASFFYSMQSQ HSIPRIDMALDSRGIPSLYQLSFRQLKRFLILH Protective antigen Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0767/768 showed significant levels of protection when compared to negative control animals [Ref1057:McNeilly et al., 2007]. TC0768 hypothetical protein Chlamydia muridarum Nigg VO_0010889 1246133 15835382 TC_0768 AE002160 NP_297141 243161 911335 911496 - hypothetical protein 3.69 5224.4 53 >NC_002620.2:911335-911496 Chlamydia muridarum Nigg, complete genome GCTAAGACGCGGAGCATGGATCAACTGAAGGAATTGCAAAAGGGTCCTCTGGATAAATGACTACCTGTCC GGAAATGATCGTTAGATTACGCTCTTTTCCCTCAGGAGTAAGCTCTGCTGTTTCCGTAGAACAGACTTCA GAAGTAGGTAAGGAGATCGGCA >NP_297141.1 hypothetical protein TC_0768 [Chlamydia muridarum str. Nigg] MPISLPTSEVCSTETAELTPEGKERNLTIISGQVVIYPEDPFAIPSVDPCSAS Protective antigen Following vaginal challenge with live Chlamydia, the groups of female BALB/c mice immunized intra-abdominally with TC0767/768 showed significant levels of protection when compared to negative control animals [Ref1057:McNeilly et al., 2007]. 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