• Vaccine Name: M. tuberculosis DNA Vaccine encoding Ag85B Protein
• Target Pathogen: Mycobacterium tuberculosis
• Target Disease: Tuberculosis
• Vaccine Ontology ID: VO_0011410
• Type: DNA vaccine
• Status: Research
• Ag85B from M. tuberculosis H37Rv gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005039
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Host Strain: C57BL/6
• Vaccination Protocol: Mice at or between 7 and 8 weeks old were immunized. Fifty micrograms of plasmid, both DNA-85B or control vector, was injected intramuscularly (i.m.) in 50 μl PBS into the hind leg. Mice were immunized two or four times at 2 week intervals. As a positive control, a single dose of BCG (105 cfu) was injected s.c. (Palma et al., 2007).
• Challenge Protocol: Four weeks after the last boost, mice were challenged intravenously (i.v.) in a lateral tail vein with 10^5 cfu of MTB H37Rv (Palma et al., 2007).
• Efficacy: A significant reduction of the bacterial load was observed in mice receiving two administrations of the DNA-85B vaccine (DNAx2-immunized mice) with respect to unvaccinated naïve control or empty-plasmid DNA recipient mice, indicating protection by the vaccine. The degree of reduction of the mycobacterial burden was not significantly different from that conferred by BCG, although the immunization with BCG caused the highest nominal reduction of mycobacteria in the lungs (Palma et al., 2007).
• Information about this animal model: Mouse Model for TB research