VIOLIN Logo
VO Banner
Search: for Help
About
Introduction
Statistics
VIOLIN News
Your VIOLIN
Register or Login
Submission
Tutorial
Vaccine & Components
Vaxquery
Vaxgen
VBLAST
Protegen
VirmugenDB
DNAVaxDB
CanVaxKB
Vaxjo
Vaxvec
Vevax
Huvax
Cov19VaxKB
Host Responses
VaximmutorDB
VIGET
Vaxafe
Vaxar
Vaxism
Vaccine Literature
VO-SciMiner
Litesearch
Vaxmesh
Vaxlert
Vaccine Design
Vaxign2
Vaxign
Community Efforts
Vaccine Ontology
ICoVax 2012
ICoVax 2013
Advisory Committee
Vaccine Society
Vaxperts
VaxPub
VaxCom
VaxLaw
VaxMedia
VaxMeet
VaxFund
VaxCareer
Data Exchange
V-Utilities
VIOLINML
Help & Documents
Publications
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UM Logo

Vaccine Detail

Infectious Bronchitis Virus DNA Vaccine encoding S1, N, and M
Vaccine Information
  • Vaccine Name: Infectious Bronchitis Virus DNA Vaccine encoding S1, N, and M
  • Target Pathogen: Infectious Bronchitis Virus (IBV)
  • Target Disease: Infectious Bronchitis
  • Vaccine Ontology ID: VO_0004584
  • Type: DNA vaccine
  • Status: Research
  • N protein gene engineering:
    • Type: DNA vaccine construction
    • Description:
    • Detailed Gene Information: Click Here.
  • S1 gene engineering:
    • Type: DNA vaccine construction
    • Description:
    • Detailed Gene Information: Click Here.
  • M membrane protein gene engineering:
    • Type: DNA vaccine construction
    • Description:
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid:
    • DNA vaccine plasmid name:
    • DNA vaccine plasmid VO ID: VO_0000024
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Chicken Response

  • Vaccination Protocol: The seven day old SPF chickens were randomly divided into six groups with 20 chickens per group. Groups 1–3 received 100 micro-g of pVAX1-S1, PVAX1-M, or pVAX1-N,
    respectively; Group 4 received 100 micro-g combined DNA vaccine containing 33 micro-g of each of the three antigen plasmids; Groups 5–6 received 100 micro-g empty pVAX1 and 0.5 ml PBS. All the chickens were immunized intramuscularly with the vaccines at seven days of age (Yang et al., 2009).
  • Vaccine Immune Response Type: VO_0003057
  • Immune Response: Anti-IBV antibody levels were detected in chickens immunized with either pVAX1-S1, pVAX1-M, pVAX1-N or the three constructs in combination. In fact, a higher antibody titer level was observed in chickens immunized with the three expression constructs in combination compared with the individual constructs, suggesting a greater potency for inducing antibody response (Yang et al., 2009).
  • Challenge Protocol: All chickens were challenged with 100EID50 of the IBV SAIBk strain in 0.1 ml by a nasal-ocular route at 21 days after the boost immunization (Yang et al., 2009).
  • Efficacy: Immunization with the multivalent DNA vaccine provided up to 85% immune protection in the vaccinated chickens (Yang et al., 2009).
References
Yang et al., 2009: Yang T, Wang HN, Wang X, Tang JN, Gao R, Li J, Guo ZC, Li YL. Multivalent DNA vaccine enhanced protection efficacy against infectious bronchitis virus in chickens. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 2009; 71(12); 1585-1590. [PubMed: 20046025].