• Vaccine Name: M. tuberculosis DNA vaccine pcDNA3.1+/Ag85A DNA encoding Ag85A
• Target Pathogen: Mycobacterium tuberculosis
• Target Disease: Tuberculosis
• Vaccine Ontology ID: VO_0004356
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• FbpA (Ag85A) gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pcDNA3.1 expressed Mycobacterium antigen 85A (Ag85A) (Wang et al., 2010).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000158
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Immune Response: Orally administrated liposomal-pcDNA3.1+/Ag85A DNA was efficiently incorporated into mucosal epithelium of the small intestine, Peyer's patches (PP). This initiated a Ag85A-specific Th1 dominant immune response, as evidenced by increased secretion of IL-2, IFN-γ, and no change of IL-4 (Wang et al., 2010)
• Efficacy: Liposomal-pcDNA3.1+/Ag85A DNA significantly increased the protection by giving a markedly reduction of TB burden in the lung, demonstrating that the TB-specific immune responses elicited by oral administration of liposomal-pcDNA3.1+/Ag85A DNA led to an enhanced host defense against TB infection. (Wang et al., 2010)
• Information about this animal model: Mouse Model for TB research