• Vaccine Name: S. pneumoniae PhpA-79 Protein Vaccine
• Target Pathogen: Streptococcus pneumoniae
• Target Disease: Pneumonia
• Vaccine Ontology ID: VO_0004063
• Type: Subunit vaccine
• Status: Research
• Antigen: Recombinant PhpA-79
• phpA gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001250
  • Description: 100 μg of monophosphoryl lipid A (MPL; Corixa, Hamilton, Mont.) per dose (Zhang et al., 2001).
• Immunization Route: subcutaneous injection

Mouse Response

• Host Strain: CBA/N
• Vaccination Protocol: CBA/N mice, at 10 animals per group, were immunized with 5 μg of either rPhpA-79 or rPhpA-20. For parenteral immunization, rPhpA-79 or rPhpA-20 protein was mixed with 100 μg of monophosphoryl lipid A (MPL; Corixa, Hamilton, Mont.) per dose to a final volume of 200 μl in saline and then injected s.c. into mice. All groups received a booster with the same dose and by the same route 3 and 5 weeks after the primary immunization. Control mice were injected with MPL alone (Zhang et al., 2001).
• Challenge Protocol: Mice were challenged with either serotype 3 or serotype 14 streptomycin-resistant S. pneumoniae (Zhang et al., 2001).
• Efficacy: Immunization with either rPhpA-79–MPL provided 100% protection against death caused by type 3 S. pneumoniae infection (Zhang et al., 2001).