• Vaccine Name: S. pyogenes M Protein Vaccine
• Target Pathogen: Streptococcus pyogenes
• Target Disease: Group A streptococcal infections, Strep throat, Scarlet fever
• Vaccine Ontology ID: VO_0004053
• Type: Subunit vaccine
• Status: Research
• Antigen: A polypeptide encompassing a conformational B cell epitope of the M protein conjugated with DT (Schulze et al., 2006).
• emm1 gene engineering:
  • Type: Recombinant protein preparation
  • Description: A polypeptide encompassing a conformational B cell epitope of the M protein (J8, positions 344–355 of the M1 sequence, accession no. X62131) was synthesised, purified and conjugated with DT (Schulze et al., 2006).
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001242
  • Description: 10 μg of CTB or 0.5 μg of the TLR2/6 agonist MALP-2 as mucosal adjuvants (Schulze et al., 2006).
• Immunization Route: intranasal immunization

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Mice were immunised by intranasal route (10 μl per nostril) with either J8-DT (M protein) (30 μg per dose) co-administered with 10 μg of CTB or 0.5 μg of the TLR2/6 agonist MALP-2 as mucosal adjuvants, on day 0, 7, 14, and 28. Control animals received PBS alone (Schulze et al., 2006).
• Challenge Protocol: Mice were intranasally challenged with 10^8 CFU of the heterologous virulent S. pyogenes strain NS192 in 40 μl of PBS (20 μl per nostril) (Schulze et al., 2006).
• Efficacy: Mice immunized with J8-DT had 60% survival following challenge (Schulze et al., 2006).