• Vaccine Name: S. agalactiae Sip Protein Vaccine
• Target Pathogen: Streptococcus agalactiae
• Target Disease: Group B streptococcus (GBS) infection
• Vaccine Ontology ID: VO_0004052
• Type: Subunit vaccine
• Status: Research
• Antigen: Recombinant Sip protein.
• Sip gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001263
  • Description: 20 μg of QuilA adjuvant (Brodeur et al., 2000).
• Immunization Route: subcutaneous injection

Mouse Response

• Host Strain: CD-1
• Vaccination Protocol: Mice were injected s.c. three times at 3-week intervals with either 20 μg of purified recombinant Sip protein in 0.1 ml of PBS mixed with 20 μg of QuilA adjuvant (Cedarlane), 15 μg of formaldehyde-killed GBS WC with 20 μg of QuilA as positive controls, or 20 μg of QuilA in PBS as a negative control (Brodeur et al., 2000).
• Challenge Protocol: To evaluate the challenge dose required for each GBS strain, between 10^4 and 10^8 CFU/mouse was injected i.p. into groups of CD-1 female mice (Brodeur et al., 2000).
• Efficacy: Immunization with the recombinant Sip protein efficiently protected CD-1 mice against deadly challenges with six GBS strains of serotypes Ia/c, Ib, II/R, III, V, and VI (Brodeur et al., 2000).