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Vaccine Detail

L. donovani HASPB1 Protein Vaccine
Vaccine Information
  • Vaccine Name: L. donovani HASPB1 Protein Vaccine
  • Target Pathogen: Leishmania donovani
  • Target Disease: Visceral leishmaniasis
  • Vaccine Ontology ID: VO_0004067
  • Type: Subunit vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: mouse
  • Antigen: Recombinant hydrophilic acylated surface protein B1 (HASPB1) (Stäger et al., 2000).
  • HASPB1 gene engineering:
    • Type: Recombinant protein preparation
    • Description:
    • Detailed Gene Information: Click Here.
  • Adjuvant:
  • Immunization Route: Subcutaneous injection
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: In the first two vaccination experiments, BALB/c mice received s.c. immunization with either 1) 10 µg rHASPB1 with 1 µg murine rIL-12; 2) 10 µg rHASPB1 in saline; 3) 10 µg SLA plus 1 µg rmIL-12; 4) 1 µg rmIL-12; and 5) saline. Three weeks later, mice were boosted with the same schedule, but the IL-12 dose was reduced to 0.5 µg. After an additional 3 wk, a final boost was given omitting IL-12. In the third vaccination experiment, mice were immunized three times at 3-wk intervals with 10 µg rHASPB1 or OVA (Sigma) (Stäger et al., 2000).
  • Challenge Protocol: All mice were challenged 3 wk after the last boost with 2 x 10^7 amastigotes, given i.v. in the lateral tail vein (Stäger et al., 2000).
  • Efficacy: rHASPB1 provided significant protection against challenge with L. donovani (Stäger et al., 2000).
References
Stäger et al., 2000: Stäger S, Smith DF, Kaye PM. Immunization with a recombinant stage-regulated surface protein from Leishmania donovani induces protection against visceral leishmaniasis. Journal of immunology (Baltimore, Md. : 1950). 2000; 165(12); 7064-7071. [PubMed: 11120835].