• Vaccine Ontology ID: VO_0004353
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Duck
• Antigen: S protein from DHBV Australian strain (Triyatni et al., 1998)
• S gene engineering:
  • Type: DNA vaccine construction
  • Detailed Gene Information: Click Here.
• Vector: pcDNA I/Amp (Triyatni et al., 1998)
• Immunization Route: Intramuscular injection (i.m.)

• Vaccine Ontology ID: VO_0011411
• Type: DNA vaccine
• Status: Research
• Antigen: Hepatitis B virus HBVgp2 pre-S1/pre-S2/S
• S gene engineering:
  • Type: DNA vaccine construction
  • Description: The pCI expression vector (Promega, Charbonières, France) was used to clone the entire DHBV large envelope gene into Not I polylinker site leading to the pCI-preS/S plasmid [5] and the DHBV core gene leading to pCI-C plasmid (Thermet, submitted). The pCI-preS/S, pCI-C and the native pCI plasmids were purified by Endotoxin Free Giga prep (Qiagen, Hilden, Germany) (Thermet et al., 2003).
  • Detailed Gene Information: Click Here.
• Vector: pCI expression vector (Promega, Charbonières, France)
• Immunization Route: Intramuscular injection (i.m.)

Ducks Response

• Vaccine Immune Response Type: VO_0000286
• Immune Response: The S-DNA vaccine was able to elicit humoral immune responses against DHBV surface proteins in ducks. In addition, The S-DNA vaccine induced high titers of anti-DHBs antibodies, and anti-S antibodies induced by the S-DNA construct were highly effective in neutralizing virus infectivity. (Triyatni et al., 1998).
• Efficacy: Vaccination of ducks with S DNA vaccines prevented the development of viremia following virus challenge. All ducks were challenged with a high-titer dose of DHBV virus. Preincubation of the virus with 5, 10, or, 20 μl of anti-S serum at 37°C for 1 hour prior to i.v. inoculation into 1-day-old ducklings completely prevented the development of viremia during a 4-week observation period in all of the ducks (Triyatni et al., 1998).

Ducks Response

• Host Strain: Pekin
• Vaccination Protocol: Four-week-old ducks received intramuscular (i.m.) injections of 100–300 μg of plasmid DNA diluted in NaCl 0.9%. The birds were injected in three sites (anterior quadriceps of both legs and breast), and booster doses were given 3 weeks later at the same sites (Thermet et al., 2003).
• Challenge Protocol: Progeny ducklings received a high titre DHBV challenge and the viremia was followed by quantitative dot blot hybridisation for each animal during 17 days (Thermet et al., 2003).
• Efficacy: Immunisation with a plasmid encoding the DHBV large (L) envelope protein (HBVgp2 pre-S1/pre-S2/S) induced a strong, specific, highly neutralising and long-lasting anti-preS humoral response in uninfected ducks. Importantly, maternal antibodies elicited by such DNA immunisation were vertically transmitted and protected progeny against viral challenge (Thermet et al., 2003).