• Vaccine Ontology ID: VO_0011515
• Type: Subunit vaccine
• Status: Research
• TM-1 gene engineering:
  • Type: Recombinant protein preparation
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • VO ID: VO_0000127
• Immunization Route: Subcutaneous injection

• Vaccine Ontology ID: VO_0002857
• Type: Live, attenuated vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Chicken
• lpd gene engineering:
  • Type: Gene mutation
  • Description: This lpd mutant is from Mycoplasma gallisepticum (Gates et al., 2008).
  • Detailed Gene Information: Click Here.
• Immunization Route: Intratracheal immunization

Chicken Response

• Host Strain: SPF
• Vaccination Protocol: Eight-week-old specific pathogen-free (SPF) chickens were immunized subcutaneously in the right thigh with the TM-1 polypeptide, suspended in a mixture of 1 ml of PBS and 1 ml of an aluminium hydroxide adjuvant (Saito et al., 1993).
• Challenge Protocol: Four weeks later, the immunized chickens were challenged intranasally with 0.5 ml of a suspension of 10^6 c.f.u, ml- 1 of M.g. KP13 strain 27 (Saito et al., 1993).
• Efficacy: Only one of five immunized chickens harboured viable M.g. cells in its sinuses after being killed 5 days after challenge with M.g. (Saito et al., 1993).

Chicken Response

• Persistence: An lpd mutant is highly attenuated in chickens (Gates et al., 2008).
• Efficacy: An lpd mutant induces significant protection in chickens from challenge with wild type Mycoplasma gallisepticum (Gates et al., 2008).
• Host IgG Fc fragment response
  • Description: Mg 7 vaccinated chickens mounted a strong humoral anti-M. gallisepticum-specific IgG response that was sustained for at least 4 weeks post-exposure. M. gallisepticum-specific immunoglobulin responses were detected in chickens inoculated with either Mg 7 or R(low) but not in chickens that received 1× PBS alone. The level of M. gallisepticum-specific serum IgG was highest in the R(low) treated animals at both the 2- and 4-week time points, however, the Mg 7 inoculated birds mounted a fairly robust response considering its level of attenuation. Thus, the Mg 7 vaccine induced significantly higher IgG responses than PBS inoculated chickens, but significantly lower than those inoculated with wild type R(low) (Gates et al., 2008).
  • Detailed Gene Information: Click Here.