• Vaccine Ontology ID: VO_0004567
• Type: DNA vaccine
• Status: Research
• N nucleocapsid protein gene engineering:
  • Type: DNA vaccine construction
  • Detailed Gene Information: Click Here.
• G glycoprotein gene engineering:
  • Type: DNA vaccine construction
  • Detailed Gene Information: Click Here.
• Vector: pcDNA-G and pcDNA-N (Seo et al., 2006)
• Immunization Route: Intramuscular injection (i.m.)

Baboon Response

• Vaccination Protocol: Five groups of forty flounder fry with a mean weight of 3 g were prepared for injection with pcDNA-G, pcDNA-N, a pcDNA-G and pcDNA-N mixture, pcDNA3.1(+) vector, or PBS (Seo et al., 2006).
• Vaccine Immune Response Type: VO_0003057
• Immune Response: Both cellular and humoral immune responses are involved in the protection of fish immunized with DNA vaccine. Induction of specific antibody response has been observed from rainbow trout immunized with rhabdovirus G protein constructs. However, despite high levels of protection, neutralizing antibodies could not be detected in all vaccinated fish at the time of challenge, or survivors of the challenge (Seo et al., 2006).
• Challenge Protocol: At 21 days post vaccination, flounder fry injected with different DNA vaccines were challenged by immersion in water containing 1.2 × 10^4 TCID ml−1 HIRRV (Seo et al., 2006).
• Efficacy: Groups injected with the mixed pcDNA-N+pcDNA-G vaccine showed only 2.5% cumulative mortality. Control groups injected with plasmid only or PBS only showed at least 95% mortality. These results demonstrated the protective effect of this DNA vaccine against HIRRV infection (Seo et al., 2006).