BV-RVG/RVG |
Rabies DNA Vaccine encoding Rabies virus Glycoprotein |
Rabies vaccine rVac-N |
Rabies virus DNA vaccine encoding the ERA glycoprotein |
Rabies virus DNA vaccine pSG5rab.gp |
Rabies virus glycoprotein G mutant vaccine |
Rabies virus P protein mutant vaccine |
rLSDV-Rabies-gP |
rORFV-RabG |
Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
- Vaccine Ontology ID: VO_0004651
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- Glycoprotein G
gene engineering:
- Type: Recombinant protein preparation
- Description: The recombinant baculovirus (BV-RVG/RVG) was pseudotyped with the rabies virus glycoprotein (RVG) and also simultaneously expressed another RVG under the control of the immediate early CMV promoter (Wu et al., 2014).
- Detailed Gene Information: Click Here.
- Preparation: Recombinant baculovirus (BV-RVG/RVG) was pseudotyped with the rabies virus glycoprotein (RVG) and also simultaneously expressed another RVG under the control of the immediate early CMV promoter (Wu et al., 2014).
- Immunization Route: Intramuscular injection (i.m.)
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- Vaccine Ontology ID: VO_0011553
- Type: DNA vaccine
- Status: Research
- G
gene engineering:
- Type: DNA vaccine construction
- Detailed Gene Information: Click Here.
- Vector: pgp.LAMP-1 (Kaur et al., 2010)
- Immunization Route: Intramuscular injection (i.m.)
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- Vaccine Ontology ID: VO_0011555
- Type: Recombinant vector vaccine
- Status: Research
- RABVgp1 nucleoprotein N
gene engineering:
- Type: Recombinant vector construction
- Detailed Gene Information: Click Here.
- Vector: Vaccinia virus (Fujii et al., 1994)
- Immunization Route: Intraperitoneal injection (i.p.)
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- Vaccine Ontology ID: VO_0004372
- Type: DNA vaccine
- Status: Research
- glycoprotein
gene engineering:
- Type: DNA vaccine construction
- Description: This DNA vaccine expressed the full-length cDNA of the ERA rabies virus glycoprotein (Lodmell et al., 2000).
- Detailed Gene Information: Click Here.
- Vector: pCMV4 (Lodmell et al., 2000)
- Immunization Route: Intramuscular injection (i.m.)
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- Vaccine Ontology ID: VO_0004326
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- glycoprotein
gene engineering:
- Type: DNA vaccine construction
- Description: Vector pSG5 expressed the rabies glycoprotein (ERA strain) (Xiang et al., 1994).
- Detailed Gene Information: Click Here.
- Vector: pSG5 (Xiang et al., 1994)
- Immunization Route: Intramuscular injection (i.m.)
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- Vaccine Ontology ID: VO_0002991
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse, hamster
- Glycoprotein G
gene engineering:
- Type: Gene mutation
- Description: This glycoprotein G mutant is from Rabies virus (Wu et al., 2011).
- Detailed Gene Information: Click Here.
- Immunization Route: Intramuscular injection (i.m.)
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- Vaccine Ontology ID: VO_0002992
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse
- P protein
gene engineering:
- Type: Gene mutation
- Description: This P protein mutant is from rabies virus (Morimoto et al., 2005).
- Detailed Gene Information: Click Here.
- Immunization Route: Intracranial immunization
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- Vaccine Ontology ID: VO_0004762
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- Glycoprotein G
gene engineering:
- Type: Recombinant vector construction
- Description: Recombinant LSDV encoding the rabies glycoprotein (rLSDV-RG) (Aspden et al., 2003).
- Detailed Gene Information: Click Here.
- Preparation: Using the rabies virus glycoprotein (RG) as a model antigen, the recombinant LSDV encoding the rabies glycoprotein (rLSDV-RG) was able to express RG (Aspden et al., 2003).
- Immunization Route: Intramuscular injection (i.m.)
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- Vaccine Ontology ID: VO_0004766
- Type: Recombinant vector vaccine
- Status: Research
- Host Species for Licensed Use: Baboon
- RABVgp1 nucleoprotein N
gene engineering:
- Type: Recombinant vector construction
- Description: Orf virus (ORFV) recombinant, D1701-V-RabG, expressing the rabies virus (RABV) glycoprotein that is correctly presented on the surface of infected cells without the need of replication or production of infectious recombinant virus(Amann et al., 2013).
- Detailed Gene Information: Click Here.
- Preparation: Orf virus (ORFV) recombinant, D1701-V-RabG, expressing the rabies virus (RABV) glycoprotein (Amann et al., 2013).
- Immunization Route: Intramuscular injection (i.m.)
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Host Response |
Host Response |
Host Response |
Host Response |
Host Response |
Host Response |
Host Response |
Host Response |
Host Response |
Mouse Response
- Vaccination Protocol: Mice were immunized intramuscularly with 1 × 10^8 IFU of BV-VSVG/EGFP, BVRVG/EGFP, or BV-RVG/RVG at 3-week interval (Wu et al., 2014).
- Vaccine Immune Response Type: VO_0003057
- Challenge Protocol: Mice were challenged intracerebrally with 50 mouse 50% lethal doses (50 LD50) of rabies virus CVS-24 (Wu et al., 2014).
- Efficacy: Mice immunized with BV-RVG/RVG developed higher levels of virus-neutralizing antibodies, and conferred 100% protection against rabies viral challenge (Wu et al., 2014).
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Mouse Response
- Vaccination Protocol: For the intramuscular route, mice were vaccinated with 100 µg of endotoxin-free pDNA in 200 µl of PBS/animal in the anterior quadriceps muscle, in the individual groups (DNA vaccine or vector control), 3 times at 3-wk intervals. Control mice were immunized with PBS only (Kaur et al., 2010).
- Challenge Protocol: For challenge studies, immunized mice were inoculated intracerebrally with 20 LD50 of the CVS strain of rabies virus 21 d after the last immunization. The challenged mice were observed for 18 d for symptoms indicative of rabies virus infection (Kaur et al., 2010).
- Efficacy: DNA vaccine imparted partial protection (60%) against challenge with 20 LD(50) of the challenge virus standard (CVS) strain of rabies virus (Kaur et al., 2010).
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Mouse Response
- Host Strain: A/J
- Vaccination Protocol: 4-week-old mice were injected intraperitoneally (i.p.) on days 0 and 14 with 10^7 p.f.u, of the recombinant viruses. Control mice received equal doses of vaccinia virus recombinant vSC8 that contained no rabies virus sequences (Fujii et al., 1994).
- Challenge Protocol: On day 21, the mice were challenged by footpad injection with 0.02 ml (20 MFPLDs0 , where MFPLDs0 represents mouse footpad 50% lethal dose) of the 1088 strain (Fujii et al., 1994).
- Efficacy: 92 % of mice given rVac-N (i.e. one animal succumbed b u t still had a prolonged lifespan) survived the challenge with rabies virus (Fujii et al., 1994).
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Mouse Response
- Vaccine Immune Response Type: VO_0000286
- Immune Response: Neutralizing antibody titers were enhanced following primary i.m. vaccination with DNA and MPL® (Lodmell et al., 2000).
- Efficacy: After virus challenge, in the group of mice that were vaccinated with MPL, 4 of 6 mice with individual neutralizing antibody titers of 1:10 survived viral challenge. In addition, in the group of mice that did not receive MPL, 100% of the mice survived viral challenge after their second booster (Lodmell et al., 2000).
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Mouse Response
- Vaccine Immune Response Type: VO_0000286
- Immune Response: Mice immunized intramuscularly with the pSG5rab.gp vector developed rabies virus glycoprotein-specific cytolytic T cells, lymphokinesecreting T helper cells of the TH1 subset, and rabies virus-neutralizing antibodies (Xiang et al., 1994).
- Efficacy: Mice vaccinated with the pSG5rab.gp vector were fully protected against a subsequent challenge with rabies virus (Xiang et al., 1994).
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Mouse Response
- Persistence: A glycoprotein G mutant is attenuated in mice (Wu et al., 2011).
- Efficacy: A glycoprotein G mutant induces protection in mice from challenge with wild type rabies virus (Wu et al., 2011).
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Mouse Response
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Mouse Response
- Vaccination Protocol: NMRI mice (3–4 weeks, n=30 per group; South African Vaccine Producers) were inoculated intramuscularly on days 0 and 14 with either PBS (50 μl), rLSDV-RG (5×10^4 f.f.u. ml−1 in 50 μl), LSDV-wt (5×10^4 f.f.u. ml−1 in 50 μl) or Verorab (1/10 human equivalent dose in 50 μl) (Aspden et al., 2003).
- Vaccine Immune Response Type: VO_0003057
- Challenge Protocol: On day 21, ten mice from each group were challenged intracranially with 8×10^−1 LD50, 8×10^−2 LD50 or 8×10^−3 LD50 dilution of live rabies virus (Aspden et al., 2003).
- Efficacy: Mice immunized with rLSDV-RG elicited levels of RV-specific cellular immunity (T-cell proliferation) comparable with those of mice immunized with a commercial inactivated rabies vaccine (Verorab; Pasteur Merieux). Most importantly, mice immunized with rLSDV-RG were protected from an aggressive intracranial rabies virus challenge (Aspden et al., 2003).
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Mouse Response
- Vaccination Protocol: Mice were i.m. immunized once, twice, or thrice with 10^7 PFU of D1701-V-RabG in 2-week intervals. Two other groups were vaccinated three times with 10^6 PFU either i.m. or s.c (Amann et al., 2013).
- Vaccine Immune Response Type: VO_0003057
- Challenge Protocol: Two weeks after the last immunization, all mice were i.c. challenged with 3,000 mouse i.c. LD50 of the highly pathogenic RABV CVS-11 strain (Amann et al., 2013).
- Efficacy: All i.m. immunized animals, receiving one, two, or three inoculations of 10^7 PFU or three doses of 10^6 PFU, were completely protected, whereas 4 out of the 5 s.c. immunized animals survived the challenge. The D1701-V-RabG-immunized and protected mice did not reveal any harmful reaction, loss of body weight, or RABV-specific clinical signs (Amann et al., 2013).
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Hamster Response
- Persistence: A glycoprotein G mutant is attenuated in hamsters (Wu et al., 2011).
- Efficacy: A glycoprotein G mutant induces significant protection in hamsters from challenge with wild type rabies virus (Wu et al., 2011).
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References |
References |
References |
References |
References |
References |
References |
References |
References |
Wu et al., 2014: Wu Q, Yu F, Xu J, Li Y, Chen H, Xiao S, Fu ZF, Fang L. Rabies-virus-glycoprotein-pseudotyped recombinant baculovirus vaccine confers complete protection against lethal rabies virus challenge in a mouse model. Veterinary microbiology. 2014; 171(1-2); 93-9101. [PubMed: 24793501].
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Kaur et al., 2010: Kaur M, Saxena A, Rai A, Bhatnagar R. Rabies DNA vaccine encoding lysosome-targeted glycoprotein supplemented with Emulsigen-D confers complete protection in preexposure and postexposure studies in BALB/c mice. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2010; 24(1); 173-183. [PubMed: 19741168].
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Fujii et al., 1994: Fujii H, Takita-Sonoda Y, Mifune K, Hirai K, Nishizono A, Mannen K. Protective efficacy in mice of post-exposure vaccination with vaccinia virus recombinant expressing either rabies virus glycoprotein or nucleoprotein. The Journal of general virology. 1994; 75 ( Pt 6); 1339-1344. [PubMed: 8207400].
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Lodmell et al., 2000: Lodmell DL, Ray NB, Ulrich JT, Ewalt LC. DNA vaccination of mice against rabies virus: effects of the route of vaccination and the adjuvant monophosphoryl lipid A (MPL). Vaccine. 2000; 18(11-12); 1059-1066. [PubMed: 10590326].
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Xiang et al., 1994: Xiang ZQ, Spitalnik S, Tran M, Wunner WH, Cheng J, Ertl HC. Vaccination with a plasmid vector carrying the rabies virus glycoprotein gene induces protective immunity against rabies virus. Virology. 1994; 199(1); 132-140. [PubMed: 8116236].
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Wu et al., 2011: Wu X, Franka R, Henderson H, Rupprecht CE. Live attenuated rabies virus co-infected with street rabies virus protects animals against rabies. Vaccine. 2011; ; . [PubMed: 21514343].
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Morimoto et al., 2005: Morimoto K, Shoji Y, Inoue S. Characterization of P gene-deficient rabies virus: propagation, pathogenicity and antigenicity. Virus research. 2005; 111(1); 61-67. [PubMed: 15896403].
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Aspden et al., 2003: Aspden K, Passmore JA, Tiedt F, Williamson AL. Evaluation of lumpy skin disease virus, a capripoxvirus, as a replication-deficient vaccine vector. The Journal of general virology. 2003; 84(Pt 8); 1985-1996. [PubMed: 12867628].
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Amann et al., 2013: Amann R, Rohde J, Wulle U, Conlee D, Raue R, Martinon O, Rziha HJ. A new rabies vaccine based on a recombinant ORF virus (parapoxvirus) expressing the rabies virus glycoprotein. Journal of virology. 2013; 87(3); 1618-1630. [PubMed: 23175365].
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