|
Porcine respiratory and reproductive syndrome virus GP5 protein mutant vaccine |
PRRSV DNA vaccine GST-ORF5 encoding GP5 |
PRRSV DNA vaccine pCA-U-optiGP5 |
PRRSV DNA vaccine pCI-ORF5/ORF6 |
PRRSV DNA vaccine pIRESorf5/IFNγ |
PRRSV DNA vaccine pIRESorf5/IL-2 |
PRRSV DNA vaccine pIRESorf7/IL-2 |
| Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
Vaccine Information |
- Product Name: HuN4-F112
- Vaccine Ontology ID: VO_0002982
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Pig
- GP5 protein
gene engineering:
- Type: Gene mutation
- Description: This GP5 protein mutant is from Porcine reproductive and respiratory syndrome virus (Tian et al., 2009).
- Detailed Gene Information: Click Here.
- Immunization Route: Intramuscular injection (i.m.)
|
- Vaccine Ontology ID: VO_0004325
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Pig, mouse
- GP5 protein
gene engineering:
- Type: DNA vaccine construction
- Description: Vector pET21a expressed the major envelope glycoprotein (GP5) of porcine reproductive and respiratory syndrome virus (PRRSV) (Pirzadeh and Dea, 1998).
- Detailed Gene Information: Click Here.
- Vector: pET21a (Pirzadeh and Dea, 1998)
- Immunization Route: Intramuscular injection (i.m.)
|
- Vaccine Ontology ID: VO_0004506
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Pig
- ORF5
gene engineering:
- Type: DNA vaccine construction
- Description: Vector pCAGGS expressed the U-optiGP5 fusion gene was generated by linking optiGP5 downstream of ubiquitin. The Kozak sequence, GCCACC, served as the upstream start codon for all three fragments (Hou et al., 2008).
- Detailed Gene Information: Click Here.
- ubiquitin
gene engineering:
- Type: DNA vaccine construction
- Description: This vaccine expressed a sequence encoding pig ubiquitin that had its Gly76 changed to Ala76 (Hou et al., 2008).
- Detailed Gene Information: Click Here.
- Vector: pCAGGS (Hou et al., 2008)
- Immunization Route: Intramuscular injection (i.m.)
|
- Vaccine Ontology ID: VO_0004510
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Mouse, pig
- GP5
gene engineering:
- Type: DNA vaccine construction
- Description: Vector pCl-neo expressed GP5 (encoded by ORF5) and M (encoded by ORF6) (Jiang et al., 2006).
- Detailed Gene Information: Click Here.
- Vector: pCI-neo (Jiang et al., 2006)
- Immunization Route: Intramuscular injection (i.m.)
|
- Vaccine Ontology ID: VO_0004507
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Pig
- ORF5
gene engineering:
- Type: DNA vaccine construction
- Description: Vector pIRES1neo expressed GP5, encoded by ORF5, the major envelope protein of the virus harboring neutralizing epitopes (Xue et al., 2004).
- Detailed Gene Information: Click Here.
- Vector: pIRES1neo (CLONTECH) (Xue et al., 2004)
- Immunization Route: Intramuscular injection (i.m.)
|
- Vaccine Ontology ID: VO_0004508
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Pig
- ORF5
gene engineering:
- Type: DNA vaccine construction
- Description: Vector pIRES1neo expressed GP5, encoded by ORF5, the major envelope protein of the virus harboring neutralizing epitopes (Xue et al., 2004).
- Detailed Gene Information: Click Here.
- Vector: pIRES1neo (CLONTECH) (Xue et al., 2004)
- Immunization Route: Intramuscular injection (i.m.)
|
- Vaccine Ontology ID: VO_0004509
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Pig
- ORF7
gene engineering:
- Type: DNA vaccine construction
- Description: Vector pIRES1neo expressed N, encoded by ORF7, which forms the nucleocapsid protein of the virus, and it is likely to contain virus-specific T cell epitopes (Xue et al., 2004).
- Detailed Gene Information: Click Here.
- Vector: pIRES1neo (Xue et al., 2004)
- Immunization Route: Intramuscular injection (i.m.)
|
| Host Response |
Host Response |
Host Response |
Host Response |
Host Response |
Host Response |
Host Response |
|
Pig Response
- Persistence: A GP5 protein mutant is attenuated in piglets (Tian et al., 2009).
- Efficacy: A GP5 protein mutant induces protection in piglets from challenge with wild type HP-PRRSV (Tian et al., 2009).
|
Pig Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: Following a massive intratracheal challenge with the virulent IAF-Klop strain of PRRSV, DNA-vaccinated pigs were protected from generalized viraemia and the development of typical macroscopic lung lesions that were observed in unvaccinated, virus-challenged controls, as well as in pigs that were immunized with E. coli-expressed GST-ORF5 recombinant fusion protein (Pirzadeh and Dea, 1998).
|
Pig Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: Four of six pigs in the pCA-U-optiGP5 group were devoid of visible pathological changes that were present in other vaccinated and control animals after challenge (Hou et al., 2008).
|
Pig Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: All immunized piglets developed neutralizing antibodies at 10 weeks after primary immunization, whereas there was no detectable neutralizing antibodies in piglets immunized with pCI-ORF5. Neutralizing antibodies are considered to be important in protection against PRRSV (Jiang et al., 2006).
|
Pig Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: After immunization and viral challenge, two of three pigs immunized with pIRESorf5/IFNgamma were protected from lung lesions that were present in other vaccinated and control animals (Cho and Dee, 2006).
|
Pig Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: After immunization and viral challenge, one of three pigs immunized with pIRESorf5/IL-2 was protected from lung lesions that were present in other vaccinated and control animals (Xue et al., 2004).
|
Pig Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: After immunization and viral challenge, one of three pigs immunized with pIRESorf7/IL-2 was protected from lung lesions that were present in other vaccinated and control animals (Xue et al., 2004).
|
| References |
References |
References |
References |
References |
References |
References |
Tian et al., 2009: Tian ZJ, An TQ, Zhou YJ, Peng JM, Hu SP, Wei TC, Jiang YF, Xiao Y, Tong GZ. An attenuated live vaccine based on highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) protects piglets against HP-PRRS. Veterinary microbiology. 2009; 138(1-2); 34-40. [PubMed: 19339125].
|
Pirzadeh and Dea, 1998: Pirzadeh B, Dea S. Immune response in pigs vaccinated with plasmid DNA encoding ORF5 of porcine reproductive and respiratory syndrome virus. The Journal of general virology. 1998; 79 ( Pt 5); 989-999. [PubMed: 9603313].
|
Hou et al., 2008: Hou YH, Chen J, Tong GZ, Tian ZJ, Zhou YJ, Li GX, Li X, Peng JM, An TQ, Yang HC. A recombinant plasmid co-expressing swine ubiquitin and the GP5 encoding-gene of porcine reproductive and respiratory syndrome virus induces protective immunity in piglets. Vaccine. 2008; 26(11); 1438-1449. [PubMed: 18262692].
|
Jiang et al., 2006: Jiang Y, Xiao S, Fang L, Yu X, Song Y, Niu C, Chen H. DNA vaccines co-expressing GP5 and M proteins of porcine reproductive and respiratory syndrome virus (PRRSV) display enhanced immunogenicity. Vaccine. 2006; 24(15); 2869-2879. [PubMed: 16446016].
|
Cho and Dee, 2006: Cho JG, Dee SA. Porcine reproductive and respiratory syndrome virus. Theriogenology. 2006; 66(3); 655-662. [PubMed: 16730057].
Xue et al., 2004: Xue Q, Zhao YG, Zhou YJ, Qiu HJ, Wang YF, Wu DL, Tian ZJ, Tong GZ. Immune responses of swine following DNA immunization with plasmids encoding porcine reproductive and respiratory syndrome virus ORFs 5 and 7, and porcine IL-2 and IFNgamma. Veterinary immunology and immunopathology. 2004; 102(3); 291-298. [PubMed: 15507312].
|
Xue et al., 2004: Xue Q, Zhao YG, Zhou YJ, Qiu HJ, Wang YF, Wu DL, Tian ZJ, Tong GZ. Immune responses of swine following DNA immunization with plasmids encoding porcine reproductive and respiratory syndrome virus ORFs 5 and 7, and porcine IL-2 and IFNgamma. Veterinary immunology and immunopathology. 2004; 102(3); 291-298. [PubMed: 15507312].
|
Xue et al., 2004: Xue Q, Zhao YG, Zhou YJ, Qiu HJ, Wang YF, Wu DL, Tian ZJ, Tong GZ. Immune responses of swine following DNA immunization with plasmids encoding porcine reproductive and respiratory syndrome virus ORFs 5 and 7, and porcine IL-2 and IFNgamma. Veterinary immunology and immunopathology. 2004; 102(3); 291-298. [PubMed: 15507312].
|
