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Bovine respiratory syncytial virus glycoprotein G mutant vaccine |
Bovine Respiratory Syncytial Virus recombinant vector vaccine BHVl/BRSVG encoding the G protein |
BRSV DNA vaccine VCL-Fb |
| Vaccine Information |
Vaccine Information |
Vaccine Information |
- Vaccine Ontology ID: VO_0002951
- Type: Live, attenuated vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Cow
- glycoprotein G
gene engineering:
- Type: Gene mutation
- Description: This glycoprotein G mutant is from Bovine respiratory syncytial virus (Schmidt et al., 2002).
- Detailed Gene Information: Click Here.
- Immunization Route: intranasal immunization
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- Vaccine Ontology ID: VO_0004289
- Type: Recombinant vector vaccine
- Status: Licensed
- Host Species as Laboratory Animal Model: Calves
- glycoprotein G
gene engineering:
- Type: Recombinant vector construction
- Description: The gE gene was replaced by a gene encoding the G protein of BRSV in a BHVI vector (Schrijver et al., 1997).
- Detailed Gene Information: Click Here.
- Vector: BHVI (Schrijver et al., 1997)
- Immunization Route: intranasal immunization
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- Vaccine Ontology ID: VO_0004574
- Type: DNA vaccine
- Status: Research
- Host Species as Laboratory Animal Model: Calves
- F
gene engineering:
- Type: DNA vaccine construction
- Detailed Gene Information: Click Here.
- Vector: pVCL1012 (Taylor et al., 2005)
- Immunization Route: Intramuscular injection (i.m.)
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| Host Response |
Host Response |
Host Response |
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Cattle Response
- Persistence: A glycoprotein G mutant is attenuated in calves (Schmidt et al., 2002).
- Efficacy: A glycoprotein G mutant induces significant protection in calves from challenge with wild type BRSV (Schmidt et al., 2002).
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Cattle Response
- Vaccine Immune Response Type: VO_0000286
- Efficacy: Flow cytometric analysis showed a significant relative increase of gamma/delta+ T cells in peripheral blood after BRSV challenge-infection of the calves of the control group but not in the vaccinated groups. These results indicate that the G protein of BRSV can induce significant protection against BRSV infection in cattle, and that the BHV1/BRSV-G vaccine protects effectively against a subsequent BRSV and BHV1 infection (Schrijver et al., 1997).
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Cattle Response
- Vaccine Immune Response Type: VO_0003057
- Immune Response: Seven days after BRSV challenge, BRSV-specific serum IgA titres in calves vaccinated i.m. with VCL-Fb were significantly greater than those in calves vaccinated by the i.d. route (P < 0.05) (Taylor et al., 2005).
- Efficacy: Vaccination with DNA encoding the BRSV F protein can induce a significant reduction in BRSV excretion in young calves. At slaughter, 7 days after challenge, BRSV was isolated from the lungs of three out of four calves vaccinated with control plasmid, two out of six calves vaccinated i.d. with VCL-Fb, but none of the calves vaccinated i.m. with VCL-Fb (Taylor et al., 2005).
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| References |
References |
References |
Schmidt et al., 2002: Schmidt U, Beyer J, Polster U, Gershwin LJ, Buchholz UJ. Mucosal immunization with live recombinant bovine respiratory syncytial virus (BRSV) and recombinant BRSV lacking the envelope glycoprotein G protects against challenge with wild-type BRSV. Journal of virology. 2002; 76(23); 12355-12359. [PubMed: 12414977].
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Schrijver et al., 1997: Schrijver RS, Langedijk JP, Keil GM, Middel WG, Maris-Veldhuis M, Van Oirschot JT, Rijsewijk FA. Immunization of cattle with a BHV1 vector vaccine or a DNA vaccine both coding for the G protein of BRSV. Vaccine. 1997; 15(17-18); 1908-1916. [PubMed: 9413101].
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Taylor et al., 2005: Taylor G, Bruce C, Barbet AF, Wyld SG, Thomas LH. DNA vaccination against respiratory syncytial virus in young calves. Vaccine. 2005; 23(10); 1242-1250. [PubMed: 15652666].
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