VIOLIN: Vaccine Investigation and Online Information Network

Streptococcus pneumoniae

Table of Contents

General Information
Vaccine Related Pathogen Genes
1. cps
2. CbpA (Protective antigen)
3. ClpP (Protective antigen)
4. DnaJ (Protective antigen)
5. endopeptidase O(PepO) (Protective antigen)
6. GltX (Protective antigen)
7. Gnd (Protective antigen)
8. LplA (Protective antigen)
9. NanA (Protective antigen)
10. pavB (Protective antigen)
11. pcpA (Protective antigen)
12. phpA (Protective antigen)
13. phtB (Protective antigen)
14. PhtD (Protective antigen)
15. phtE (Protective antigen)
16. PiaA (Protective antigen)
17. PiuA (Protective antigen)
18. Ply (Protective antigen, Virmugen)
19. PlyD1 (Protective antigen)
20. prtA (Protective antigen)
21. psaA (Protective antigen)
22. PspA (Protective antigen)
23. pspA (Protective antigen)
24. pspK (Protective antigen)
25. psrP (Protective antigen)
26. SrtA (Protective antigen)
Vaccine Related Host Genes
1. IgA
2. IgG
Vaccine Information
References

I. General Information

1. NCBI Taxonomy ID:

1313

2. Disease:

Pneumonia

3. Introduction

Streptococcus pneumoniae, or pneumococcus, is a Gram-positive, alpha-hemolytic diplococcus aerotolerant anaerobe and a member of the genus Streptococcus. S. pneumoniae is the most common cause of both pneumonia overall and fatal pneumonia. Antibiotic resistance has developed worldwide and is most frequent in pneumococcal serotypes that are most prevalent in children (types/groups 6, 14, 19, and 23). The incidence of pneumococcal disease is the highest in children < 2 years of age and in adults > 65 years of age. Other important risk factors are chronic heart and lung disease, cigarette smoking, and asplenia (Ortqvist et al., 2005). Other than pneumonia, it also causes acute sinusitis, otitis media, meningitis, bacteremia, osteomyelitis, septic arthritis, endocarditis, peritonitis, pericarditis, cellulitis, and brain abscess.

4. Microbial Pathogenesis

S. pneumoniae is normally found in the nasopharynx. It attaches to nasopharyngeal cells through interaction of bacterial surface adhesins. It can cause otitis media or sinusitis if it enters areas such as the Eustachian tube or nasal sinuses. Pneumonia occurs if the organisms are inhaled into the lungs and not eliminated. S. pneumoniae can activate complement, stimulate cytokine production, and attracts neutrophils and other white blood cells. The organism's polysaccharide capsule makes it resistant to phagocytosis. S. pneumoniae can spread to other areas and cause various diseases. S. pneumoniae has several virulence factors, including the polysaccharide capsule, pneumococcal surface proteins, and IgA1 protease (Wiki: S. pneumoniae).

5. Host Ranges and Animal Models

Pneumococci spontaneously cause disease in humans, monkeys, rabbits, horses, mice and guinea pigs. Nasopharyngeal colonization occurs in approximately 40% of the population (Textbook of Bacteriology).

6. Host Protective Immunity

The bacteria invade and grow primarily due to their resistance to the host phagocytic response. The cell wall components directly activate multiple inflammatory cascades, including the alternative pathway of complement activation, the coagulation cascade and the cytokine cascade, inducing interleukin-1, interleukin-6 and tumor necrosis factor (TNF) from macrophages and other cells (Textbook of Bacteriology).

II. Vaccine Related Pathogen Genes

1. CbpA

Gene Name : CbpA
Sequence Strain (Species/Organism) : Streptococcus pneumoniae D39
VO ID : VO_0011203
NCBI Gene ID : 4441159
NCBI Protein GI : 116515359
Locus Tag : SPD_2017
Genbank Accession : CP000410
Protein Accession : YP_817402
3D structure: PDB ID : 1W9R
Taxonomy ID : 373153
Gene Starting Position : 1995043
Gene Ending Position : 1997148
Gene Strand (Orientation) : -
Protein Name : choline binding protein A
Protein pI : 8.27
Protein Weight : 75722.56
Protein Length : 701
Protein Note : equivalent gene in S.pneumoniae R6 = spr1995 This gene is also called SpsA, pspC (pneumococcal surface protein C)and Hic; identified by match to protein family HMM PF01473; match to protein family HMM PF04650; match to protein family HMM PF05062; match to protein family HMM TIGR01168

DNA Sequence : Show Sequence

>NC_008533.1:1995043-1997148 Streptococcus pneumoniae D39, complete genome
TTTAGTTTACCCATTCACCATTGGCATTGACTCCATAGCCATCTACAGTTGTGTTGACTGCAAGGGCACC
TGAGCCATTGACATAGTACCATTTATCTGATACTTTGAACCATTGGCTTGCTTTCATAGCACCTGATGCT
TCAAGATAGTACCAGGTATCTCCATCTTTCACCCAACCTGTCGCCATATCACCATTAGCGTTGAGGTAAT
ACCATGAACCGTTGTATTGGAGCCATCCTGTCGCCATATCACCATTAGCGTTGAGGTAGTACCATGAACC
GTTGTTTTGGAGCCATCCTGTCGCCATATCACCATTAGCGTTGAGGTAGTACCATGAGCCATTGTATTGG
AGCCATCCTGTCGCCATAGCGCCATTGCTGTTTAGGTAGTACCATGAACCATTGTATTGGAGCCATCCTG
TCGCCATAGCACCATTAGCGTTTAGGTAGTACCATGAACCATTGTTTTGGAGCCATCCTGTTGCCATTGA
ACCATTAGCGTTTAGATAGTACCATGAACCATTGTTTTGGAGCCATCCTGTCGCCATAGCACCATTAGCG
TTTAGATAGTACCATGAACCGTTGTTTTGGAGCCATCCTGTTGCCATTGAACCATCAGTATTGTAGAAGT
ACCACATACCGTTTTCTTGTTTCCAGCCTGTTTTTGGAGTAGATGGTTGTGCTGGTTTTTCAGTTTTTGG
CGGTTGCTGTTGAGTCAAGCGATTATATTCTTCTTCTGATCTACGAGCATAGTCTTCTTCAGCTTGTTGA
TCATCTGTTTTTTCTGCTTTTGGTTGTTCAGCTGGCTTCTCTGGTTTTGGAGCTGGTTTTTCTGGTTGAG
TAGCCGGCGCTGGTTGTGGTTGTTCAGCTGGTTTTTCTTTAACTTTATCTTCTTCTGCTGCTTTTCGTTT
AGCTTCTTCTTCTGCTTTTTTACGATCTGTCTTGATGTTTTCTAACCTTGTAGCCTCAGCTTTTTTACTC
TCAACTTTCGCTTTTGCTTGCTTAATTTTTTCCTCGTCTCGAGGTTCCTTAGCTTCCTCTTTTACTAGTT
CAAGCTCCGCTTCTTTAACTTTCACATCGGACTCAGCAATTTCAAGGTCAAGCGTTTTGTAAGTATTGGT
TGGGTAGTTACGGCGATCTTCTTCTTTTTGATCCTTGGCTTTTTTCTCAGCTTCTTCAACCTTCTTCTCA
GCTTCTGCTACCTTTTTTCCTGATTTCAGGGATGAGCTTGGAAGAGTTTCTTCACCTACGCTAGAATCTG
AAGACTTCGCATCATTTTCTTTTTTATCAGGTGTTGCTAGCTCTCCAGGAACTCCTCGTTTTGCCCGCCC
CTTTGGTTTACCTTGATCTGAAGTCGCTACATTAGCTTCCTTCAACTTAGCATCTGCTTTTCGTTTAGCT
TCTTCTTCTGCTTTTTTACGATCTGTCTTGATGTTTTCTAACCTTGTAGCCTCAGCTTTTTTACTCTCAA
CTTTCTCTTTTGCTTGCTTAATTGTGCCCTCGTTTCGAGATTCTTTAGCTTCCTCTTTTACTAGTTCAAG
CTCCGCTTCTTTAACTTTCACATCGAACTCAGCAATTTCAAGTTCAAGCGTTTTGTAAGTATTGGTTGGG
TAGTTACGACGATCTTCTTCTTTTTGATCCTCGGCTTTTTTCTTAGCTTCTTCAACCTTCTTCTTAGCTT
CTGCTACCTTTTCTCCTGGTTTCAATGTATCTTTTTTAAACTTCTCAAAAGCTGCGTCTAACTTTGCTTT
TATTTCTGACGGCAACTCATCTTTCGACTTCTCTTCTAAAACATTTAATTCACGCAAATACTTCGTTTTA
ATTGCGCTCAACTTTATGTTTAAGGCGACATTTTGGGTATGTTTTCTTCTATCTAGTTGAATCTCCCTCA
ACATTTTTTCTATATATTCATCGACGACTTGTTTAGCAGCTTTCCTATGTTCTGTCTTTGCCATATTAGA
AGAAGTGGCTGCTTGGGTACTTCCCTCGTTCTCTGTCGCATGAACCACACTTCCCATAACAAGACTGGCA
ACAGCTACACTAGCTACTCCAATACTAAATTTACGAATTGAATAATGTACTTTTCTTTCGCTTTTTGATG
CAAACA

Protein Sequence : Show Sequence

>YP_817402.1 choline binding protein A [Streptococcus pneumoniae D39]
MFASKSERKVHYSIRKFSIGVASVAVASLVMGSVVHATENEGSTQAATSSNMAKTEHRKAAKQVVDEYIE
KMLREIQLDRRKHTQNVALNIKLSAIKTKYLRELNVLEEKSKDELPSEIKAKLDAAFEKFKKDTLKPGEK
VAEAKKKVEEAKKKAEDQKEEDRRNYPTNTYKTLELEIAEFDVKVKEAELELVKEEAKESRNEGTIKQAK
EKVESKKAEATRLENIKTDRKKAEEEAKRKADAKLKEANVATSDQGKPKGRAKRGVPGELATPDKKENDA
KSSDSSVGEETLPSSSLKSGKKVAEAEKKVEEAEKKAKDQKEEDRRNYPTNTYKTLDLEIAESDVKVKEA
ELELVKEEAKEPRDEEKIKQAKAKVESKKAEATRLENIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPA
PATQPEKPAPKPEKPAEQPKAEKTDDQQAEEDYARRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENG
MWYFYNTDGSMATGWLQNNGSWYYLNANGAMATGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNANGA
MATGWLQYNGSWYYLNSNGAMATGWLQYNGSWYYLNANGDMATGWLQNNGSWYYLNANGDMATGWLQYNG
SWYYLNANGDMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDGYGVNANGEWV
N

Molecule Role : Protective antigen
Molecule Role Annotation : Mice were actively immunized with a fragment of choline binding protein A (CbpA; also known as PspC, Hic, and SpsA). CbpA was able to protect mice from intraperitoneal challenge with medium to very high doses of a highly virulent capsular type 2 pneumococcal strain, D39 (Ogunniyi et al., 2001).
Related Vaccine(s): S. pneumoniae CbpA Protein Vaccine

2. ClpP

Gene Name : ClpP
Sequence Strain (Species/Organism) : Streptococcus pneumoniae D39
VO ID : VO_0011193
NCBI Gene ID : 4441476
NCBI Protein GI : WP_000613477
Locus Tag : SPD_0650
Genbank Accession : CP000410
Protein Accession : YP_816144
3D structure: PDB ID : 1Y7O
Other Database IDs : CDD:178955
CDD:132928
Taxonomy ID : 1301
Gene Starting Position : 668966
Gene Ending Position : 669556
Gene Strand (Orientation) : +
Protein Name : ATP-dependent Clp protease proteolytic subunit
Protein pI : 4.37
Protein Weight : 22213.63
Protein Length : 293
Protein Note : ATP-dependent Clp protease proteolytic subunit; Reviewed; PRK00277

DNA Sequence : Show Sequence

>NC_008533.1:668966-669556 Streptococcus pneumoniae D39, complete genome
AATGATTCCTGTAGTTATTGAACAAACAAGCCGTGGAGAACGTTCTTACGATATTTACTCACGTCTTCTC
AAAGACCGCATCATTATGCTGACAGGTCCGGTTGAAGACAATATGGCTAACTCTGTTATTGCCCAACTGC
TTTTCTTGGATGCCCAAGATAGTACAAAAGATATTTACCTTTATGTCAATACACCAGGTGGTTCTGTTTC
AGCTGGTTTGGCAATCGTAGATACCATGAACTTTATCAAGGCAGATGTCCAAACCATTGTTATGGGAATG
GCTGCATCTATGGGAACAGTCATCGCATCAAGTGGAGCAAAAGGCAAACGTTTCATGCTTCCAAATGCTG
AATACATGATTCACCAACCAATGGGCGGTACAGGTGGTGGTACCCAACAAACTGATATGGCTATCGCTGC
AGAACACTTGCTCAAAACTCGTAATACCTTGGAAAAAATCTTGGCTGAAAATTCAGGTCAGTCAATGGAA
AAAGTCCATGCAGATGCAGAACGTGATAACTGGATGAGCGCCCAGGAAACACTTGAATATGGCTTTATTG
ATGAAATTATGGCCAACAATTCATTGAACTA

Protein Sequence : Show Sequence

>WP_000613477.1 MULTISPECIES: ATP-dependent Clp protease proteolytic subunit [Streptococcus]
MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIAQLLFLDAQDSTKDIYLYVNTPGGSVS
AGLAIVDTMNFIKADVQTIVMGMAASMGTVIASSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAA
EHLLKTRNTLEKILAENSGQSMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN

Molecule Role : Protective antigen
Molecule Role Annotation : The protective effects of ATP-dependent caseinolytic protease (ClpP) was evaluated. Vaccinated mice were intraperitoneally and/or intranasally challenged with different pneumococcal strains. In intraperitoneal challenge models with pneumococcal strain D39 (serotype 2), clpP elicited some protection in mice (Wu et al., 2010).
Related Vaccine(s): S. pneumoniae ClpP protein Vaccine

3. cps

Gene Name : cps

4. DnaJ

Gene Name : DnaJ
NCBI Protein GI : WP_001035362
Other Database IDs : CDD:331332
CDD:227588
CDD:332379
Taxonomy ID : 1313
Gene Strand (Orientation) : ?
Protein Name : hypothetical protein
Protein pI : 4.54
Protein Weight : 79758.9
Protein Length : 820
Protein Note : Gap junction protein N-terminal region; cl26511

Protein Sequence : Show Sequence

>WP_001035362.1 hypothetical protein [Streptococcus pneumoniae]
MNKKKMILTSLASVAILGAGFVTSQPTFVRAEESPQVVEKSSLEKKYEEAKAKADTAKKDYETAKKKAED
AQKKYEDDQKRTEEKARKEAEASQKLNDVALVVQNAYKEYREVQNQRSKYKSDAEYQKKLTEVDSKIEKA
RKEQQDLQNKFNEVRAVVVPEPNALAETKKKAEEAKAEEKVAKRKYDYATLKVALAKKEVEAKELEIEKL
QYEISTLEQEVATAQHQVDNLKKLLAGADPDDGTEVIEAKLKKGEAELNAKQAELAKKQTELEKLLDSLD
PEGKTQDELDKEAEEAELDKKADELQNKVADLEKEISNLEILLGGADPEDDTAALQNKLAAKKAELAKKQ
TELEKLLDSLDPEGKTQDELDKEAEEAELDKKADELQNKVADLEKEISNLEILLGGADSEDDTAALQNKL
ATKKAELEKTQKELDAALNELGPDGDEEETPAPAPQPEQPAPAPKPEQPAPAPKPEQPAPAPKPEQPAPA
PKPEQPAPAPKPEQPAKPEKPAEEPTQPEKPATPKTGWKQENGMWYFYNTDGSMAIGWLQNNGSWYYLNA
NGAMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNSNGAMATGWLQYNGSWYYLNANGDMATGWL
QYNGSWYYLNANGDMATGWAKVNGSWYYLNANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLE
ASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWV

Molecule Role : Protective antigen
Molecule Role Annotation : All the control mice died within 2 days of post-infection, while 70% of animals immunized with DnaJ survived the lethal challenge by S. pneumoniae. The study reveals that immunization of mice with DnaJ elicits protective immunity against S. pneumoniae infection.(Khan et al., 2006)
Related Vaccine(s): S. pneumoniae DnaJ Protein Vaccine

5. endopeptidase O(PepO)

Gene Name : endopeptidase O(PepO)
Sequence Strain (Species/Organism) : Streptococcus pneumoniae
NCBI Protein GI : EJG73599
Taxonomy ID : 914147
Protein Name : endopeptidase O
Protein pI : 4.43
Protein Weight : 68202.47
Protein Length : 703

Protein Sequence : Show Sequence

>EJG73599.1 endopeptidase O [Streptococcus pneumoniae 2082239]
MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLMLATTDKWLAGEEVPEDAILENFVKYHR
LVRDFDKREADGITPVLPLLKEFQELETFADFTAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPS
TILPDTTYYAEEHPQREELLTLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAK
LYHPYSYEDFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVVNLST
SYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSPEAKADVEKKVATMIDV
YKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKDKVVNETASLFENALAFARVEIKHSWSKW
NQPVDYKEWGMPAHMVNAYYNPQKNLIVFPAAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGAS
FDENGSLKDWWTESDYAAFKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFS
AEEFFYNFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPEERVIIW

Molecule Role : Protective antigen
Molecule Role Annotation : The results of colonization experiment showed that compared with the control group, the PepO, PsaA, and combined immunization groups showed a significant reduction in the colonization of Streptococcus pneumoniae (CMCC31693 and CMCC31207) in the nasopharynx and lung (P<0.05).(Zhang et al., 2017)

6. GltX

Gene Name : GltX
Sequence Strain (Species/Organism) : Streptococcus pneumoniae TIGR4
VO ID : VO_0011201
NCBI Gene ID : 931970
NCBI Protein GI : WP_000031087
Locus Tag : SP_2069
Genbank Accession : AE005672
Protein Accession : NP_346492
Other Database IDs : CDD:234953
Taxonomy ID : 1313
Gene Starting Position : 1976626
Gene Ending Position : 1978086
Gene Strand (Orientation) : -
Protein Name : glutamate--tRNA ligase
Protein pI : 4.67
Protein Weight : 54587.89
Protein Length : 560
Protein Note : glutamyl-tRNA synthetase; Reviewed; PRK01406

DNA Sequence : Show Sequence

>NC_003028.3:1976626-1978086 Streptococcus pneumoniae TIGR4, complete genome
TTTATTTAGAGATTTCTTTTAGCATGTTTTCGATATGCTGAATTGATTTTTCACGTCCAAGCAAGAAAAT
TGTATCTGGTAATTCTGGCCCATGCATTTCGCCTGAAACTGCGATACGAATAGGCATGAAAAGATTTTTC
CCTTTAATACCTGTTTCTTTTTGGACTGCTTTAATTTGTGGGAAGATATTTTCTGTCACAAATTCATCAT
CTGTCATCGCTTCAAGTTTTGCTTTGAATGCTTCAAGAACTGTTGGAACTGTTTCACCCGTCATGACTTC
GCGCTCTGCTTCTGTCAATTCTGGGAAATCTGAGAAGAAAAGATCTGTCAATGGGATAATCTCATCTACT
GATTTCATTTGTGGTTTATAGAGCTCAACTAATTTTTCAGCCTTGTCAGTCAAACGGCCTGCTTCCTCTA
AGAATGGTTTTGCCATTTCAAAGATGGTTTCAAGGTCTGCATTCTTGATATAATCATTGCTCATCCAGTC
TAGTTTTTTCTGATCAAAGGCTGCTGGTGACTTGCTGAGGCGGTTTTCATCAAAAAGTTTAATGAATTCT
TCACGAGAGAAAATCTCATCTTCACCACCTGGGTTCCAACCAAGAAGAGCAATAAAGTTAAAGACTGCTT
CTGGAAGGTAACCTTTCTTTCGGTAATCTTCGATAAATTGAAGTGTATTAGTATCACGTTTAGATAACTT
CTTACCAGTTTCAGAGTTGATAATCAAGGTCATGTGACCGAACTCTGGAGCTTCCCAACCAAGAGCTTCA
TAGACCATAAGCTGTTTTGGTGTATTAGCAATATGGTCATCTCCACGGATAACATGAGAGATTTGCATAT
CGTGGTCATCGATAACAACGGCAAAGTTGTAAGTTGGGTAACCGTCTTTCTTTTGGATAACCCAGTCACC
ACCGATATTGCCACCTTCAAATTCGATATCGCCTTTGACCATATCATGCCACTTGTAGATACCTGACTCA
TTGACAGCCAAACGAACAGTTGGGATGATCCCTGCTGCTTCACGTTCTGCGATGTAAGCTGCTTTTTCTT
CTTCACTCATACCAAGGTATTCATTGATGTAGCGTGGTGTTTCGCCAGCTACTTCTTGGCGTTCGCGTTC
AGCTGCCAACTCTTCTTCTGTAACGTAAGATTTATAGGCTTTTCCTTCAGCTAATAGTTGGTCAATATAT
TTTTGATACAAGTCCAAACGCTCAGACTGGCGATAATTCTCATGTGATTCTGGACTTTCATCCCAATCCA
TGCCTAACCAGCGAAGGTTTTCAAGTTGTGAACGTTCACCATCCTCGACATGGCGTTTACGGTCAGTATC
TTCGATACGGATGAGAAATGTTCCACCATGATGGCGCGCATACAAGTAATTAAACAATGCTGTACGAGCA
TTTCCGATGTGTAGTAGTCCTGTTGGACTTGGTGCGTAACGTACGCGGATATCTTTTGACA

Protein Sequence : Show Sequence

>WP_000031087.1 glutamate--tRNA ligase [Streptococcus pneumoniae]
MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIEDTDRKRHVEDGERSQLENLRWLGMDW
DESPESHENYRQSERLDLYQKYIDQLLAEGKAYKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEK
AAYIAEREAAGIIPTVRLAVNESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQ
ISHVIRGDDHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYLPEAV
FNFIALLGWNPGGEDEIFSREEFIKLFDENRLSKSPAAFDQKKLDWMSNDYIKNADLETIFEMAKPFLEE
AGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEAEREVMTGETVPTVLEAFKAKLEAMTDDE
FVTENIFPQIKAVQKETGIKGKNLFMPIRIAVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK

Molecule Role : Protective antigen
Molecule Role Annotation : Glutamyl tRNA synthetase (GtS) has been found to be among the Streptococcus pneumoniae cell wall-derived proteins that have age-dependent immunogenicity in children. Here, GtS was cloned, expressed, and purified and then was used to immunize 7-week-old BALB/c OlaHsd mice. Thirty-nine percent of rGtS-immunized mice survived a lethal bacterial challenge, whereas no control mice survived (Mizrachi et al., 2007).
Related Vaccine(s): S. pneumoniae GtS Protein Vaccine

7. Gnd

Gene Name : Gnd
Sequence Strain (Species/Organism) : Streptococcus pneumoniae D39
VO ID : VO_0011206
NCBI Gene ID : 4441177
NCBI Protein GI : WP_000158781
Locus Tag : SPD_0343
Genbank Accession : CP000410
Protein Accession : YP_815856
Other Database IDs : CDD:236453
Taxonomy ID : 1301
Gene Starting Position : 347261
Gene Ending Position : 348685
Gene Strand (Orientation) : +
Protein Name : NADP-dependent phosphogluconate dehydrogenase
Protein pI : 4.58
Protein Weight : 51871.44
Protein Length : 574
Protein Note : 6-phosphogluconate dehydrogenase; Validated

DNA Sequence : Show Sequence

>NC_008533.1:347261-348685 Streptococcus pneumoniae D39, complete genome
AATGACAAAAGCTAACTTTGGTGTCGTAGGTATGGCCGTAATGGGTCGTAACCTTGCCCTTAATATTGAA
TCACGTGGTTACACAGTTGCTATCTACAACCGTAGTAAAGAAAAAACGGAAGATGTGATTGCTTGCCATC
CTGAAAAGAATTTTGTACCAAGCTATGACGTTGAAAGTTTTGTAAACTCAATCGAAAAACCTCGTCGTAT
CATGCTGATGGTTCAAGCTGGACCTGGTACAGATGCTACTATTCAAGCCCTTCTTCCGCACCTTGACAAG
GGTGATATCTTGATTGACGGTGGAAATACTTTCTACAAAGATACCATCCGTCGTAATGAAGAATTGGCAA
ACTCTGGTATCAACTTTATCGGTACTGGGGTTTCTGGTGGTGAAAAAGGTGCCCTTGAAGGTCCTTCTAT
CATGCCTGGTGGACAAAAAGAAGCCTACGAATTGGTTGCGGATGTTCTTGAAGAAATCTCAGCTAAAGCA
CCAGAAGATGGCAAACCATGTGTGACTTACATCGGTCCTGATGGAGCTGGTCACTATGTGAAAATGGTTC
ACAATGGTATTGAGTACGGTGATATGCAATTGATCGCAGAAAGCTATGACTTGATGCAACACTTGCTAGG
CCTTTCTGCAGAAGATATGGCTGAAATCTTTACTGAGTGGAACAAGGGTGAATTAGACAGCTACTTGATT
GAAATCACAGCTGATATCTTGAGCCGTAAAGACGATGAAGGCCAAGATGGACCAATCGTAGACTACATCC
TTGATGCTGCAGGTAACAAGGGAACTGGTAAATGGACTAGCCAATCATCTCTTGACCTTGGTGTACCATT
GTCACTGATTACTGAGTCAGTGTTTGCACGCTACATTTCAACTTACAAAGAAGAACGTGTACATGCTAGC
AAGGTGCTTCCAAAACCAGCTGCCTTCAACTTTGAAGGAGACAAGGCTGAATTGATTGAAAAGATCCGTC
AAGCCCTTTACTTCTCAAAAATCATTTCATACGCACAAGGATTTGCTCAATTGCGTGTAGCCTCTAAAGA
AAACAACTGGAACTTGCCATTTGCAGATATCGCATCTATCTGGCGTGATGGCTGTATCATCCGTTCTCGT
TTCTTGCAAAAGATTACAGATGCTTACAACCGCGATGCAGATCTTGCCAACCTTCTTTTGGACGAGTACT
TCTTGGATGTTACTGCTAAGTACCAACAAGCAGTACGTGATATCGTAGCTCTTGCGGTTCAAGCAGGTGT
GCCAGTGCCAACTTTCTCAGCAGCTATTACTTACTTTGATAGCTACCGTTCAGCTGACCTTCCAGCTAAC
TTGATCCAAGCACAACGTGACTACTTTGGTGCTCACACTTACCAACGTAAAGACAAAGAAGGAACCTTCC
ACTACTCTTGGTATGACGAAAAATA

Protein Sequence : Show Sequence

>WP_000158781.1 MULTISPECIES: NADP-dependent phosphogluconate dehydrogenase [Streptococcus]
MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIACHPEKNFVPSYDVESFVNSIEKPRRI
MLMVQAGPGTDATIQALLPHLDKGDILIDGGNTFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSI
MPGGQKEAYELVADVLEEISAKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLG
LSAEDMAEIFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDLGVPL
SLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKIISYAQGFAQLRVASKE
NNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLLLDEYFLDVTAKYQQAVRDIVALAVQAGV
PVPTFSAAITYFDSYRSADLPANLIQAQRDYFGAHTYQRKDKEGTFHYSWYDEK

Molecule Role : Protective antigen
Molecule Role Annotation : BALB/c mice were immunized with recombinant 6-Phosphogluconate dehydrogenase (r6PGD) and boosted after 3 weeks. Immunized mice were challenged intranasally with a lethal dose of S. pneumoniae. Immunization of mice with r6PGD protected 60% (P < 0.001) of mice for 5 days and 40% (P < 0.05) of the mice for 21 days following intranasal lethal challenge (Daniely et al., 2006).
Related Vaccine(s): S. pneumoniae 6PGD Protein Vaccine

8. LplA

Gene Name : LplA
Sequence Strain (Species/Organism) : Streptococcus pneumoniae R6
VO ID : VO_0011194
NCBI Gene ID : 934072
NCBI Protein GI : 15903091
Locus Tag : spr1047
Genbank Accession : AE007317
Protein Accession : NP_358641
Taxonomy ID : 171101
Gene Starting Position : 1041220
Gene Ending Position : 1042209
Gene Strand (Orientation) : -
Protein Name : lipoate-protein ligase
Protein pI : 5.54
Protein Weight : 35144.06
Protein Length : 329
Protein Note : Putative

DNA Sequence : Show Sequence

>NC_003098.1:1041220-1042209 Streptococcus pneumoniae R6 chromosome, complete genome
TTTATCCAACTACCGCTTCAGCGATTTCTTCACGGCTAATACCAGCGAAGTAGCGTGTGATATCAATGGT
TTTTAGCGCCTTAAGAACATCTTCGCGTTCGTATTTCACCCCACGAAGGACATCTTCTACTGCAGCAACG
TCTTCAATACCAAAGAAGTCACCATAAATCTTGATGTCTTGGATTTTTGATTCAGTAACGTTAGCAAAGA
CTTCAACCTTACCACTAGTGAATTTGGTTCCACGACGGACGTTAAATTCAGGTGATTTACCATAGTTCCA
GTCCCAAGTTCCAAACTTAGTATCCTTGATGCGATTGATTTCGGCCAATTCTTCTTCTGAAAAGACGTAT
TCAGTCATCTCTGGGTACTCTTTTTTCATGTATTCCAAGAGTAAATCACGGAATTTTTCGACTGTGATTT
TTTTTGGTAATTCATTGATAATATTGGTTACACGGGCACGGACGGATTTCACACCTTTTGATTCAAATTT
ATCTTTTGAAACCTTAAGGGCATTTGCGAGGACTGACAAATCAACGTCAAAGAGCAAGCAACCGTGGTGC
ATGATACGGCCGTTGATATAGGCTTGGGCATTGCCACAGAACTTCTTACCATCAATCTCAAGGTCATTAC
GACCTGTGAACTCAGCTTTAACCCCAAGTTGAGCCAGGGTATTGATAACCGGAGTTGAGAAGCTCTTGAA
GTCAAATGCCTTATTTTCATCTTCTTTGGAGATGATCGTGTAGTTGAGGTTATTTAAATCGTGGTAAACA
GCTCCACCACCACTAATACGGCGAACTACCTCAATACCATTTTCGCGAACATAATCACGGTTGATTTCTT
CGATAGTGTTCTGGTGACGACCAACAATGATAGATGGCTTGTTAATCCAAAGTAGGAAGATTTGATCCTC
ATCCAAAAGGTGTTTAAAGGCGTATTCTTCCAAGGCAATATTAAAAGCAGTGTCATTTGAATGATTGATA
ATGTATTTCA

Protein Sequence : Show Sequence

>NP_358641.1 lipoate-protein ligase [Streptococcus pneumoniae R6]
MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVGRHQNTIEEINRDYVRENGIEVVRRIS
GGGAVYHDLNNLNYTIISKEDENKAFDFKSFSTPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYIN
GRIMHHGCLLFDVDLSVLANALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYP
EMTEYVFSEEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIYGDFF
GIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG

Molecule Role : Protective antigen
Molecule Role Annotation : The production of a recombinant vaccine was achieved following PCR amplification of putative lipoate protein ligase (Lpl), with subsequent cloning and expression of the genes in E. coli. The putative lipoate protein ligase (Lpl) , resulted in a reduced CFU titre and a trend towards reduced mortality in an animal sepsis model (Morsczeck et al., 2008).

9. NanA

Gene Name : NanA
Sequence Strain (Species/Organism) : Streptococcus pneumoniae
NCBI Protein GI : ARD37933
Taxonomy ID : 1313
Protein Name : NanA
Protein pI : 7.81
Protein Weight : 8529.03
Protein Length : 123

Protein Sequence : Show Sequence

>ARD37933.1 NanA [Streptococcus pneumoniae]
MLLTSSVLSATSKQCFELTSSVLSTTSKQCFELTSSVLSTTSKRCFEQPVASFLVCSLIFIEYKELGNRL
FLYCVY

Molecule Role : Protective antigen
Molecule Role Annotation : Seven proteins induced protective responses, in terms of reduced lung burdens of the serotype 3 pneumococci. Three of the protective proteins were histidine triad protein family members (PhtB, PhtD and PhtE). Four other proteins, all bearing LPXTG linkage domains, also had activity in this model (PrtA, NanA, PavB and Eng).(Anderson et al., 2016)

10. pavB

Gene Name : pavB
Sequence Strain (Species/Organism) : Streptococcus pneumoniae
NCBI Protein GI : EJH25875
Taxonomy ID : 760841
Protein Name : pavB protein
Protein pI : 4.71
Protein Weight : 54654.09
Protein Length : 622

Protein Sequence : Show Sequence

>EJH25875.1 pavB protein [Streptococcus pneumoniae GA47562]
MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADVVNPTPGQVLPEETSGTKEGDLSEKPG
DTVLTQAKPEGVTGNTNSLPTPTERTEVSEETNSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQ
ASPAETTPEQVKGGVKENTKDSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAP
WSDNGTAKNPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGKADLT
NLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLEKAKGEGPFTAGVNHVIPYELFAGDGMLTR
LLLKASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQFRANGTQTYSATVN
VYGNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSST
NTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLKNKKEEN

Molecule Role : Protective antigen
Molecule Role Annotation : Seven proteins induced protective responses, in terms of reduced lung burdens of the serotype 3 pneumococci. Three of the protective proteins were histidine triad protein family members (PhtB, PhtD and PhtE). Four other proteins, all bearing LPXTG linkage domains, also had activity in this model (PrtA, NanA, PavB and Eng).(Anderson et al., 2016)

11. pcpA

Gene Name : pcpA
Sequence Strain (Species/Organism) : Streptococcus pneumoniae
NCBI Protein GI : WP_130894716
Other Database IDs : CDD:315882
CDD:275383
CDD:227588
Taxonomy ID : 1313
Gene Strand (Orientation) : ?
Protein Name : choline-binding protein PcpA
Protein pI : 7.18
Protein Weight : 65803.48
Protein Length : 703
Protein Note : Leucine rich repeats (6 copies); pfam13306

Protein Sequence : Show Sequence

>WP_130894716.1 choline-binding protein PcpA [Streptococcus pneumoniae]
MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSIIQQNNIKYKVLTVEGNIGTVQVGNGVT
PVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGS
KAKTIIFDKGSQLEKIEDRAFDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFAN
LSNLEKLTLPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKTPK
ETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERLAFYGNLELKELILPDN
VKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHIKNKSTEFSVKKDTFAIPETVKFYVTS
EHIKDVLKSNLSTSNDIIVEKVDNIKQETDVAKPKKNSNQGVVGWVKDKGLWYYLNESGSMATGWVKDKG
LWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGS
MATGWFKDKGLWYYLNESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG

Molecule Role : Protective antigen
Molecule Role Annotation : Vaccination with PcpA significantly reduced the bacterial burdens in MEF at both 24 (p=0.02) and 48hpi (p=0.004)(Xu et al., 2017)

12. phpA

Gene Name : phpA
Sequence Strain (Species/Organism) : Streptococcus pneumoniae
VO ID : VO_0012383
NCBI Protein GI : NP_358654
Other Database IDs : CDD:273578
CDD:282170
CDD:293251
Taxonomy ID : 171101
Gene Strand (Orientation) : ?
Protein Name : histidine motif-containing protein
Protein pI : 5.38
Protein Weight : 92561.31
Protein Length : 947
Protein Note : streptococcal histidine triad protein; TIGR01363

Protein Sequence : Show Sequence

>NP_358654.1 histidine motif-containing protein [Streptococcus pneumoniae R6]
MNQIYLRKEERMKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGKQATQKTENLTPDEVS
KREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEELLMKDPNYQLKDEDIISEIKGGYVIKV
DGKYYVYLKDAAHADNVRTKEEINRQKQEHSQHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIED
TGDAYIVPHGDHYHYIPKNELSASELAAAKAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNT
NTSNNSNTNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLIFDPAQITSRTANGVAVPHGDHYHFIPYSQ
LSPLEEKLARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSPQPAPNPQPAPSNPIDEKLVKEAVRKVGD
GYVFEENGVPRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLARIHQDLL
DNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKYT
TEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKG
AEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYSLEDLLATV
KYYVEHPNERPHSDNGFGNASDHVQRNKNGQADTNQTEKPNEEKPQTEKPEEETPREEKPQSEKPESPKP
TEEPEEESPEESPEESEEPQVETEKVKEKLREAEDLLGKIQNPIIKSNAKETLTGLKNNLLFGTQDNNTI
MAEAEKLLALLKESK

Molecule Role : Protective antigen
Molecule Role Annotation : Immunization with rPhpA-79 protein adjuvanted with monophosphoryl lipid A (for subcutaneous immunization) or a mutant cholera toxin, CT-E29H (for intranasal immunization), protected CBA/N mice against death and bacteremia, as well as reduced nasopharyngeal colonization, following intranasal challenge with a heterologous pneumococcal strain (Zhang et al., 2001).
Related Vaccine(s): S. pneumoniae PhpA-79 Protein Vaccine

13. phtB

Gene Name : phtB
Sequence Strain (Species/Organism) : Streptococcus pneumoniae
NCBI Protein GI : KPL42694
Other Database IDs : CDD:273578; CDD:282170; CDD:293251; CDD:114603
Taxonomy ID : 257758
Gene Strand (Orientation) : ?
Protein Name : pneumococcal histidine triad protein B
Protein pI : 5.5
Protein Weight : 108278.97
Protein Length : 1129
Protein Note : Derived by automated computational analysis using gene prediction method: Protein Homology.

Protein Sequence : Show Sequence

>KPL42694.1 pneumococcal histidine triad protein B [Streptococcus pseudopneumoniae]
MKINKKYLAGSVAVLALSVCSYELGRYQAGQAKKESNRVAYIDGNQAGQKAENLTPDEVSKREGINAEQI
VIKITDQGYVTSHGDHYHYYNGKVPYDAIISEELLMKDSNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKD
AAHADNIRTKEEIKRQKQEHSSNHGGGSNNQAVVAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNH
FHYIPKSDLSASELAAAQAFLSGKGGQLSTVEYRSSRGETRSSVRTSPSETPQNLTTTPESQNENLASLL
QELYALALSQRHVESDGLVFDPAQITKRTANGVAVPHGDHFHFIPYSQMSALEEKLARNLPIGGQPVQKH
SDNTKPSSPAKPSSAIKANFNLNTQAPNRATGGAYTTDDGYVFSPTDVIEDTGDAFVVPHGNHFHYIPKS
DLSAGELAAAQAYWNGKQGSRPSSSSSHNANPAQPSLSENHNLTVTPTYHQNQGEDIPSLLRELYAKPLS
ERHVESDGLVFDPAQITNRTANGVAVPHGDHYHFIPYSQMSPLEEKLARMIAIKGQNGAVLPGMNYLKPA
PKPQAQPSTEKKQTDFTVEQVVRKVGEGYVVEIAGVSHYVFAKDLAKDKIDAIENLLSKKTQETHALVAK
KENVASRDQEFYDKAYNLLTQAHKALSENKGRVSDFQTLDKLAERLNDESSNKVKLVDDLLAFLAPITHP
ERLGKPNAQIAYTDDEIKLAKLAGKYTTEDGYIFDEHDIESDEGNAYVTPHLNHSHWIKKDSLSEAERAA
AQAYAKEKGLQAPNSTEATEAKPEATGTEAIYNRVKAEKKVPLEKIPNHLQYTVAVQNGHFIIPHFDHYH
NLKFAWFDEGLYSAPEGYTLEDLFATVKYYVEHPDQRPHSDNGWGNASDHVRKNKADQNGKPDEDKEHDE
VSEPTHSESDEKENHAGLNPSADNLYRPSTDAEETEEEAEDTTDEDEVPQVERSVIDVKIAEAETLLEKV
TDSRTRQNAMETLSGLNSSLLFGTKDSNTISAELDRLLALLKESQPTPI

Molecule Role : Protective antigen
Molecule Role Annotation : Seven proteins induced protective responses, in terms of reduced lung burdens of the serotype 3 pneumococci. Three of the protective proteins were histidine triad protein family members.(Anderson et al., 2016)

14. PhtD

Gene Name : PhtD
Sequence Strain (Species/Organism) : Streptococcus pneumoniae
NCBI Protein GI : WP_000700338
Other Database IDs : CDD:273578
CDD:309414
Taxonomy ID : 1313
Gene Strand (Orientation) : ?
Protein Name : pneumococcal histidine triad protein PhtD
Protein pI : 5.29
Protein Weight : 89912.36
Protein Length : 936
Protein Note : streptococcal histidine triad protein; TIGR01363

Protein Sequence : Show Sequence

>WP_000700338.1 pneumococcal histidine triad protein PhtD [Streptococcus pneumoniae]
MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDGDQAGQKAENLTPDEVSKREGINAEQI
VIKITDQGYVTSHGDHYHYYNGKVPYDAIISEELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKD
AAHADNIRTKEEIKRQKQERSHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHF
HYIPKSDLSASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSLLREL
YAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEERIARIIPLRYRSNHWVPDS
RPEEPSPQPTPKPSPSPQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAG
IDSKLAKQESLSHKLGTKKTDLPSSDREFYNKAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSD
KVKLVEDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHM
THSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQY
TVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGFGNASDHVR
KNKADQDSKPDEDKGHDEVSEPTHPESDEKENHAGLNPSADNLYKPSTDTEETEEEAEDTTDETEIPQVE
NSVINAKIAEAEALLEKVTDSSIRQNAMETLTGLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ

Molecule Role : Protective antigen
Molecule Role Annotation : Seven proteins induced protective responses, in terms of reduced lung burdens of the serotype 3 pneumococci. Three of the protective proteins were histidine triad protein family members.(Anderson et al., 2016)
Vaccination with PhtD reduced BG7322 bacterial burdens in MEF at both 24 (p=0.05) and 48hpi (p=0.16).(Xu et al., 2017)

15. phtE

Gene Name : phtE
Sequence Strain (Species/Organism) : Streptococcus pneumoniae
NCBI Protein GI : CKA60496
Other Database IDs : CDD:331999
CDD:309414
Taxonomy ID : 1313
Gene Strand (Orientation) : ?
Protein Name : pneumococcal histidine triad protein E
Protein pI : 5.25
Protein Weight : 125033.92
Protein Length : 1276
Protein Note : Streptococcal histidine triad protein; cl27178

Protein Sequence : Show Sequence

>CKA60496.1 pneumococcal histidine triad protein E [Streptococcus pneumoniae]
MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDGSQSSQKSENLTPDQVSQKEGIQAEQI
VIKITDQGYVTSHGDHYHYYNGKVPYDALFSEELLMKDPNYQLKDGDIVNEVKGGYIIKVDGKYYVYLKD
AAHADNVRTKDEINRQKQEHVKDNEKVSSDVAVARSQGRYTTDDGYVFNPADIIEDTGDAYIVPHGGHYH
YIPKSDLSASELAAAKAHLAGKNTQPSQLSYSSAASDNNTQSVAQGSTSKPESKVENLQSLLKELYDLPS
DQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPIGGTGSTVSTNAKPNAV
ASSLGSLSNNPSSLTTSRELSSTSDGYIFNPKDIVEETATAYIVRHGDHFHYIPKSNQIGQPTLPNNGLT
TPSPSLPINPGTSHEEHEEDGHGFDANRIIAEDAAGFIMTHGNHNHYFFKKDLTADQIKAAQDHLKGANT
ATPNPAHDDDHDEDHHGHHHGHHHDEDHDHGFDADRVISEDDQGFVMTHGNHNHYFFKKDLTAEQIKAAQ
DHLKAHHGAEPVKPLAKTVESFSRDASDEEKIAYISKTYGVPLEAIRISNGFFVFGNPDQAYDPTHIHPY
AVRKEHVRLPLQTGNPELDFLNELYTTALRDGVSPYSLQVENGSFVIPHGDHNHYIKVQTKGYEVALKNK
IPALQSNYQPGAFDEKGVLAKVDQLLAESRNVYKDDPIKQRQIELALGQFTENMKKLATNSTAGYLKTLD
LFDKQYIHIDESVKPVETSALDKKYQALIDKINTLDTDSYGLPKKDLLVRLQEAKLAKDEAALAAVESQL
QALQDFNDRTGDTSVEYIKYFYEHVNDSRLSDELRNKVANLTFTLYQSQSFRKAVDLNKLFPSIYQTKQE
VEEALKNQPATAKVNETVLDKEKVDNQSAKQAIYEFLKDLYPDLKKEEHVNHVSKEEVESLLSKASQLLE
QIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQVKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDI
LISLHSYLENNKGSDEDFDKVDALLDQLSAKSKDKAALLELTKAILVLNHQIQSKASASEETSPARNAEA
NGDNSSAENQPNATAESNSETASDENKPSNTRDSKPTEPALENETTESSTTTGNQAKPAE

Molecule Role : Protective antigen
Molecule Role Annotation : Seven proteins induced protective responses, in terms of reduced lung burdens of the serotype 3 pneumococci. Three of the protective proteins were histidine triad protein family members (PhtB, PhtD and PhtE). Four other proteins, all bearing LPXTG linkage domains, also had activity in this model (PrtA, NanA, PavB and Eng).(Anderson et al., 2016)

16. PiaA

Gene Name : PiaA
Sequence Strain (Species/Organism) : Streptococcus pneumoniae D39
VO ID : VO_0011207
NCBI Gene ID : 4442863
NCBI Protein GI : WP_000731179
Locus Tag : SPD_0226
Genbank Accession : CP000410
Protein Accession : YP_815744
Other Database IDs : CDD:328725
Taxonomy ID : 1313
Gene Starting Position : 216000
Gene Ending Position : 216368
Gene Strand (Orientation) : -
Protein Name : extracellular solute-binding protein
Protein pI : 4.84
Protein Weight : 35132.62
Protein Length : 424
Protein Note : Type 2 periplasmic binding fold superfamily; cl21456

DNA Sequence : Show Sequence

>NC_008533.1:216000-216368 Streptococcus pneumoniae D39, complete genome
TTTACTGTTTAGATTGGATATCTGTAAAGACTTCGTTGTATTTCTTAACGATATCTGATTTATTCTTGAT
GACATAATCATAATCTTCAGTGAGTGTTTTGATTTTGTCAATTGGTTTCATGTTTTCGCTTGTTTTAGCA
TTTTTACGAACAGGACGGTTAGTAGTGGTTGTACCAAGTGTATCTTGTACTTCTTGAGAGATAATAAAAT
CGATAAATTTCTTGGCATTTTCCATATTTTTAGATTTTTTAACGATAGCAGCACTAGCAGGTAGGAAGAC
GGTTCCTTCTTTTGGATAGACTACTTTAATGTTAGCTCCGTCATTTAAGAGTTTAACTGCTGAATCTTCA
TAAGAGAGACCAACAGTCA

Protein Sequence : Show Sequence

>WP_000731179.1 extracellular solute-binding protein [Streptococcus pneumoniae]
MKKKWMYYAACSSNESADDSSSDKGDGGSLVVYSPNSEGLIGATIPAFEEKYGIKVELIQAGTGELFKKL
ESEKEVPVADVIFGGSYTQYTTHGELFENYTSKENDNVIKEYQNTTGYSTPYTLDGSVLIVNPDLTKGMN
IEGYNDLFKPELKGKIATADPANSSSAFAQLTNMLQAQGGYKDDKAWSYVKDLFTLIDGKIGSSSSSVYK
VVADGEMAVGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDT
LGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ

Molecule Role : Protective antigen
Molecule Role Annotation : Mice immunized with recombinant PiaA were protected against systemic challenge to a degree similar to those immunized with an existing protein vaccine candidate, PdB (a genetically modified pneumolysin toxoid). Immunization with a combination of both PiuA and PiaA resulted in additive protection and was highly protective against systemic infection with S. pneumoniae (Brown et al., 2001).
Related Vaccine(s): S. pneumoniae PiaA Protein Vaccine

17. PiuA

Gene Name : PiuA
NCBI Protein GI : WP_000724932
Other Database IDs : CDD:270428
CDD:278898
Taxonomy ID : 1313
Gene Strand (Orientation) : ?
Protein Name : amino acid ABC transporter substrate-binding protein
Protein pI : 4.88
Protein Weight : 29139.71
Protein Length : 377
Protein Note : Substrate binding domain of an ABC transporter TcyJKLMN; the type 2 periplasmic binding protein fold; cd13710

Protein Sequence : Show Sequence

>WP_000724932.1 amino acid ABC transporter substrate-binding protein [Streptococcus pneumoniae]
MKKIVKYSSLAALALVAAGVLAACSGGAKKEGEAASKKEIIVATNGSPKPFIYEENGELTGYEIEVVRAI
FKDSDKYDVKFEKTEWSGVFAGLDADRYNMAVNNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSL
DDIGGKSTEVVQATTSAKQLEAYNAEHTDNPTILNYTKADLQQIMVRLSDGQFDYKIFDKIGVETVIKNQ
GLDNLKVIELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK

Molecule Role : Protective antigen
Molecule Role Annotation : Immunization with a combination of both PiuA and PiaA resulted in additive protection and was highly protective against systemic infection with S. pneumoniae.(Brown et al., 2001)

18. Ply

Gene Name : Ply
Sequence Strain (Species/Organism) : Streptococcus pneumoniae D39
VO ID : VO_0011192
NCBI Gene ID : 4441611
NCBI Protein GI : 116515376
Locus Tag : SPD_1726
Genbank Accession : CP000410
Protein Accession : YP_817150
Taxonomy ID : 373153
Gene Starting Position : 1721456
Gene Ending Position : 1722871
Gene Strand (Orientation) : -
Protein Name : pneumolysin
Protein pI : 4.91
Protein Weight : 48435.22
Protein Length : 471
Protein Note : equivalent gene in S.pneumoniae TIGR4 = SP1923; equivalent gene in S.pneumoniae R6 = spr1739; identified by match to protein family HMM PF01289

DNA Sequence : Show Sequence

>NC_008533.1:1721456-1722871 Streptococcus pneumoniae D39, complete genome
CCTAGTCATTTTCTACCTTATCCTCTACCTGAGGATAGAGAGTTGTTCCCCAAATAGAAATCGTCCGCTT
ACGCACTAGTGGCAAATCGGTTTTTTCATAAACCGTACGCCACCATTCCCAGGCAAGCCCGGTACACTCT
CTAATTTTGACAGAGAGATTACGAACATTCCCTTTTAAAGGAATACTAGTGGTAAAGTGAGCCGTCAAAT
CCTGCCCATTTCTGTCCCAAGCCTTAGGAGTCAAGACTTCCTTACCTTGATGATCATAGGATAATTCATC
CCAAGTAATATAATATTGGGCAACATAGGCACCACTATGATCCAGCAGTAAATCTCCGTTTCTGTAAGCT
GTAACCTTAGTCTCAACATAGTCTGTACTGTTTTGAAAGGTCGCAACTACATTGTCACGTAAAAAAGAAG
TTGTATAGGAAATCGGCAAGCCTGGATGATCTGCTGTAAAGCGACTGCCTTCTTGAATCAAGTCCTCTAC
CATATCCACCTTGCCTGTTACAACTCGGGCACCCGAACTTGGGTCGCCCCCTAAAATAACCGCCTTCACT
TCTGTATTGTCCAAAATCTGCTTCCACTCTGTCTGAGGAGCTACCTTGACTCCTTTTATCAAAGCTTCAA
AAGCAGCCTCTACTTCATCACTCTTACTCGTGGTTTCCAACTTGAGATAGACTTGGCGCCCATAAGCAAC
ACTCGAAATATAGACCAAAGGACGCTCTGCAGAAATTCCTCTCTGTTTTAAATCCTCTACCGTTACAGTA
TCTTGAAACACATCTCCTGGATTTTTAACAGCGTCTACGCTGACTGTATAATAAATCTGCTTAAAATTAA
CAATCTGAATCTGCTTTTCACCTGAATGGACAGAGTTAAAATCAATATCAAGAGAATTCCCTGTCTTTTC
AAAGTCAGAACCAAACTTGACCTTGAGTTGTTCCATGCTGTGAGCCGTTATTTTTTCATACTGCATTCTA
GCTGGGACATTATTGACCTGACCATAATCTTGATGCCACTTAGCCAACAAATCGTTTACCGCTCCGCGAA
CACTTGAATTGCTGGGGTCTTCCACTTGGAGAAAGCTATCGCTACTTGCCAAACCAGGCAAATCAATACT
ATAAGTCATCGGAGCACGATCAACCGCAAGAAGAGTGGGATTATTCTCTAACAAGGTCTCATCCACTACG
AGAAGTGCTCCAGGATAGAGGCGACTGTCGTTGGTAGCTGTTACAGAAATATCACTTGTATTTGTCGACA
AGCTCCGCTTCTTTCTTTCGATAACAACAAACTCATCGGGTAGCTGATTACCCTCTTTGATGAAACGATT
TTCAATACTTTCTCCCTGATGGGTCAAGAGTTTCTTTTTATCGTAATTCATAGCTAGTATAAAGTCATTT
ACTGCTTTATTTGCCA

Protein Sequence : Show Sequence

>YP_817150.1 pneumolysin [Streptococcus pneumoniae D39]
MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRL
YPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQ
VNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNP
GDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQ
ILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDY
VETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRN
LSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND

Molecule Role : Protective antigen
Molecule Role Annotation : Immunization with the pneumococcal proteins pneumolysin (Ply), choline binding protein A (CbpA), or pneumococcal surface protein A (PspA) elicits protective responses against invasive pneumococcal disease in animal models.(Chen et al., 2015)
Additional Molecule Role : Virmugen
Additional Molecule Role Annotation : The protective effects a pneumolysin mutant (DeltaA146 Ply) against pneumococcal infections in mice was evaluated. Vaccinated mice were challenged with pneumococcal strain D39 at 1,500 times the LD50 (1.5 x 105 CFU) and the vaccinated mice survived significantly longer than control mice after challenge (Wu et al., 2010).

19. PlyD1

Gene Name : PlyD1
Gene Strand (Orientation) : ?

Protein Sequence : Show Sequence

>detoxified pneumolysin [Streptococcus pneumoniae]
MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTACNDSRL
YPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQ
VNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNP
GDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQ
ILDNTEVKAVILGCDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDY
VETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRN
LSVKIREATGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND

Molecule Role : Protective antigen
Molecule Role Annotation : Results have shown that PlyD1 immunization protected mice against lethal intranasal (i.n.) challenge with pneumococci and lung injury mediated by PLY challenge. (Salha et al., 2012)
PlyD1 significantly reduced bacterial burden in MEF at 48 hpi (p = 0.02)(Xu et al., 2017)
Related Vaccine(s): S. pneumoniae detoxified PlyD1 Protein Vaccine

20. prtA

Gene Name : prtA
Sequence Strain (Species/Organism) : Streptococcus pneumoniae
NCBI Protein GI : NP_358155
Other Database IDs : CDD:310481
CDD:173801
CDD:240123
CDD:310703
CDD:273478
Taxonomy ID : 171101
Gene Strand (Orientation) : ?
Protein Name : cell wall-associated serine proteinase PrtA
Protein pI : 6.04
Protein Weight : 229148.76
Protein Length : 2263
Protein Note : Peptidase inhibitor I9; pfam05922

Protein Sequence : Show Sequence

>NP_358155.1 cell wall-associated serine proteinase PrtA [Streptococcus pneumoniae R6]
MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKVVVDKETENKEKHKDIHNAIETSKDTE
EKKTTIIEEKEVVSKNPVIDTKTSNEEAKIKEENSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKE
SGEKAIKGLSNLKNTKVLYTYDRIFNGSAIETTPDNLDKIKQIEGISSIERAQKVQPMMNHARKEIGVEE
AIDYLKSINAPFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHA
FNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSYKMYSDAGSGFA
GDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAGIPMVVATGNYATSASSSSWDLVA
NNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSK
LKFVYIGKGQDQDLIGLDLRGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMG
YEADEGTKSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKEI
DFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKSTYGYMSGTSMATPI
VAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTARPMMDATSWKEKSQYFASPRQQGAGL
INVANALRNEVVATFKNTDSKGLVNSYGSISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTD
RLKLDETYKDEKSPDGKQIVPEIHPEKVKGANITFEHGTFTIGANSSFDLNAVINVGEAKNKNKFVESFI
HFESVEEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIPGTL
NKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSGSKIANIYPLDSNGNPQDAQ
LERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVISREHFIRGILNSKSNDAKGIKSSKLKVWGDLK
WDGLIYNPRGREENAPESKDNQDPATKIRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKI
VSVDFSNPEKIKLITKDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEV
TYAGEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFADQADEKGMISYYLVDP
DKDASKYEKLGEISEDKLKNAKSPEENTNNNQAKDEDSKPDEKSSVEGEASLEINKTISTIREFENKDLK
KLIKKKFREVNDFTSETGKRIEEYDYKYDDKGNIIAYDDGSALQYETEKFDEIKSKIYGVLSPSKDGHFE
ILGKISNVSKNAKVYYGNSYKSIEIKATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKA
ETKIRMPEKNKETKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQAVLMSALDGFN
IIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEAIKEDGSMLFIDTKPVNLSM
DKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGD
IMDWGMKDYKANGFPDKVTDMDGNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGK
SVVFNINDKRNNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGE
VSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYVINLSKDTFI
KPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDHSQKSDSTKDVTATVLDKNNIS
SKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLGLKKKNQD

Molecule Role : Protective antigen
Molecule Role Annotation : Seven proteins induced protective responses, in terms of reduced lung burdens of the serotype 3 pneumococci. Three of the protective proteins were histidine triad protein family members (PhtB, PhtD and PhtE). Four other proteins, all bearing LPXTG linkage domains, also had activity in this model (PrtA, NanA, PavB and Eng).(Anderson et al., 2016)

21. psaA

Gene Name : psaA
Sequence Strain (Species/Organism) : Streptococcus pneumoniae
VO ID : VO_0011190
NCBI Protein GI : NP_359087
3D structure: PDB ID : 1PSZ
Other Database IDs : CDD:238557
Taxonomy ID : 171101
Gene Strand (Orientation) : ?
Protein Name : ABC transporter substrate-binding protein - manganese transport
Protein pI : 5.19
Protein Weight : 33579.58
Protein Length : 421
Protein Note : Metal binding protein PsaA. These proteins have been shown to function as initial receptors in ABC transport of Mn2+ and as surface adhesins in some eubacterial species. They belong to the TroA superfamily of periplasmic metal binding proteins that...; cd01137

Protein Sequence : Show Sequence

>AAL00298.1 ABC transporter substrate-binding protein - manganese transport [Streptococcus pneumoniae R6]
MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIADITKNIAGDKIDLHSIVPIGQDPHEYE
PLPEDVKKTSEADLIFYNGINLETGGNAWFTKLVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHA
WLNLENGIIFAKNIAKQLSAKDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKY
FSKAYGVPSAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQIFTD
SIAEQGKEGDSYYSMMKYNLDKIAEGLAK

Molecule Role : Protective antigen
Molecule Role Annotation : Vaccination with an RASV synthesizing full-length PsaA induced high titers of anti-PsaA antibodies in both systemic (IgG in serum) and mucosal (IgA in vaginal washes, nasal washes, and lung homogenates) sites. BALB/c (haplotype H2(d)) or C57BL/6 (haplotype H2(b)) mice vaccinated either orally or intranasally exhibited a significant reduction in colonization of nasopharyngeal tissues after intranasal challenge with S. pneumoniae strains compared to controls, although protection was not observed with all challenge strains. None of the vaccine constructs provided protection against intraperitoneal challenge with S. pneumoniae strain WU2 (serotype 3) (Wang et al., 2010).
The results of colonization experiment showed that compared with the control group, the PepO, PsaA, and combined immunization groups showed a significant reduction in the colonization of Streptococcus pneumoniae (CMCC31693 and CMCC31207) in the nasopharynx and lung (P<0.05).(Zhang et al., 2017)
Related Vaccine(s): PsaA DNA Vaccine , S. pneumoniae RASV synthesizing PsaA

22. PspA

Gene Name : PspA
Sequence Strain (Species/Organism) : Streptococcus pneumoniae D39
VO ID : VO_0011204
NCBI Gene ID : 4441373
NCBI Protein GI : 116515876
Locus Tag : SPD_0126
Genbank Accession : CP000410
Protein Accession : YP_815641
Taxonomy ID : 373153
Gene Starting Position : 128355
Gene Ending Position : 130214
Gene Strand (Orientation) : +
Protein Name : surface protein A
Protein pI : 4.82
Protein Weight : 66023.6
Protein Length : 619
Protein Note : equivalent gene in S.pneumoniae R6 = spr0121; identified by match to protein family HMM PF01473

DNA Sequence : Show Sequence

>NC_008533.1:128355-130214 Streptococcus pneumoniae D39, complete genome
GATGAATAAGAAAAAAATGATTTTAACAAGTCTAGCCAGCGTCGCTATCTTAGGGGCTGGTTTTGTTGCG
TCTCAGCCTACTGTTGTAAGAGCAGAAGAATCTCCCGTAGCCAGTCAGTCTAAAGCTGAGAAAGACTATG
ATGCAGCGAAGAAAGATGCTAAGAATGCGAAAAAAGCAGTAGAAGATGCTCAAAAGGCTTTAGATGATGC
AAAAGCTGCTCAGAAAAAATATGACGAGGATCAGAAGAAAACTGAGGAGAAAGCCGCGCTAGAAAAAGCA
GCGTCTGAAGAGATGGATAAGGCAGTGGCAGCAGTTCAACAAGCGTATCTAGCCTATCAACAAGCTACAG
ACAAAGCCGCAAAAGACGCAGCAGATAAGATGATAGATGAAGCTAAGAAACGCGAAGAAGAGGCAAAAAC
TAAATTTAATACTGTTCGAGCAATGGTAGTTCCTGAGCCAGAGCAGTTGGCTGAGACTAAGAAAAAATCA
GAAGAAGCTAAACAAAAAGCACCAGAACTTACTAAAAAACTAGAAGAAGCTAAAGCAAAATTAGAAGAGG
CTGAGAAAAAAGCTACTGAAGCCAAACAAAAAGTGGATGCTGAAGAAGTCGCTCCTCAAGCTAAAATCGC
TGAATTGGAAAATCAAGTTCATAGACTAGAACAAGAGCTCAAAGAGATTGATGAGTCTGAATCAGAAGAT
TATGCTAAAGAAGGTTTCCGTGCTCCTCTTCAATCTAAATTGGATGCCAAAAAAGCTAAACTATCAAAAC
TTGAAGAGTTAAGTGATAAGATTGATGAGTTAGACGCTGAAATTGCAAAACTTGAAGATCAACTTAAAGC
TGCTGAAGAAAACAATAATGTAGAAGACTACTTTAAAGAAGGTTTAGAGAAAACTATTGCTGCTAAAAAA
GCTGAATTAGAAAAAACTGAAGCTGACCTTAAGAAAGCAGTTAATGAGCCAGAAAAACCAGCTCCAGCTC
CAGAAACTCCAGCCCCAGAAGCACCAGCTGAACAACCAAAACCAGCGCCGGCTCCTCAACCAGCTCCCGC
ACCAAAACCAGAGAAGCCAGCTGAACAACCAAAACCAGAAAAAACAGATGATCAACAAGCTGAAGAAGAC
TATGCTCGTAGATCAGAAGAAGAATATAATCGCTTGACTCAACAGCAACCGCCAAAAGCTGAAAAACCAG
CTCCTGCACCAAAAACAGGCTGGAAACAAGAAAACGGTATGTGGTACTTCTACAATACTGATGGTTCAAT
GGCGACAGGATGGCTCCAAAACAACGGTTCATGGTACTACCTCAACAGCAATGGTGCTATGGCTACAGGT
TGGCTCCAATACAATGGTTCATGGTATTACCTCAACGCTAACGGCGCTATGGCAACAGGTTGGGCTAAAG
TCAACGGTTCATGGTACTACCTCAACGCTAATGGTGCTATGGCTACAGGTTGGCTCCAATACAACGGTTC
ATGGTATTACCTCAACGCTAACGGCGCTATGGCAACAGGTTGGGCTAAAGTCAACGGTTCATGGTACTAC
CTCAACGCTAATGGTGCTATGGCTACAGGTTGGCTCCAATACAACGGTTCATGGTACTACCTCAACGCTA
ACGGTGCTATGGCTACAGGTTGGGCTAAAGTCAACGGTTCATGGTACTACCTCAACGCTAATGGTGCTAT
GGCAACAGGTTGGGTGAAAGATGGAGATACCTGGTACTATCTTGAAGCATCAGGTGCTATGAAAGCAAGC
CAATGGTTCAAAGTATCAGATAAATGGTACTATGTCAATGGTTTAGGTGCCCTTGCAGTCAACACAACTG
TAGATGGCTATAAAGTCAATGCCAATGGTGAATGGGTTTA

Protein Sequence : Show Sequence

>YP_815641.1 surface protein A [Streptococcus pneumoniae D39]
MNKKKMILTSLASVAILGAGFVASQPTVVRAEESPVASQSKAEKDYDAAKKDAKNAKKAVEDAQKALDDA
KAAQKKYDEDQKKTEEKAALEKAASEEMDKAVAAVQQAYLAYQQATDKAAKDAADKMIDEAKKREEEAKT
KFNTVRAMVVPEPEQLAETKKKSEEAKQKAPELTKKLEEAKAKLEEAEKKATEAKQKVDAEEVAPQAKIA
ELENQVHRLEQELKEIDESESEDYAKEGFRAPLQSKLDAKKAKLSKLEELSDKIDELDAEIAKLEDQLKA
AEENNNVEDYFKEGLEKTIAAKKAELEKTEADLKKAVNEPEKPAPAPETPAPEAPAEQPKPAPAPQPAPA
PKPEKPAEQPKPEKTDDQQAEEDYARRSEEEYNRLTQQQPPKAEKPAPAPKTGWKQENGMWYFYNTDGSM
ATGWLQNNGSWYYLNSNGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGWLQYNGS
WYYLNANGAMATGWAKVNGSWYYLNANGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGAM
ATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWV

Molecule Role : Protective antigen
Molecule Role Annotation : Groups of BALB/c mice immunized with choline binding protein PspA were challenged with either D39 or WCH16. PspA elicited protection by using moderate challenge doses of S. pneumoniae (Ogunniyi et al., 2007).
Related Vaccine(s): DNA vaccine expressing PspA , S. pneumoniae PspA Protein Vaccine , S. pneumoniae pspA-rBCG Vector Vaccine

23. pspA

Gene Name : pspA
Sequence Strain (Species/Organism) : Streptococcus pneumoniae
NCBI Protein GI : AAF27715
Other Database IDs : CDD:299863
Taxonomy ID : 1313
Protein Name : PspA
Protein pI : 4.59
Protein Weight : 49428.4
Protein Length : 522
Protein Note : The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature; cl12013

Protein Sequence : Show Sequence

>AAF27715.1 PspA, partial [Streptococcus pneumoniae]
MNKKKMILTSLASVAILGTGFVASSPTFVRAEESPQVVEKSSLEKKYEEAKAKADTAKKDYETAKKKAED
AQKKYDEDQKKTEDKAKAVKKVDEERQKANLAVQKAYVEYREAKDKASAEKKIEEAKRKQKEANKKFNEE
QAKVVPEAKELAATKQKAEKAKKDAEVAKEKYDKAVQEVEVEKNKILEQDAENEKKIDVLQNKVADLEKG
IAPYQNKVAELNKEIARLQSDLKDAEENNVEDYIKEGLEQAIADKKAELATTQQNIDKTQKDLEDAELEL
EKVLATLDPEGKTQDELDKEAAEDANIEALQNKVADLENKVAELDKEVTRLQSDLKDAEENNVEDYVKEG
LEKALTDKKVELNNTQKALDTAPKALDTALNELGPDGDEEETPAPAPKPEQPAEQPKPAPAPKPEKTDDQ
QAEEDYARRSEEEYNRLPQQQPPKAEKPAPAPKPEQPVPAP

Molecule Role : Protective antigen
Molecule Role Annotation : (Tostes et al., 2017)

24. pspK

Gene Name : pspK
Sequence Strain (Species/Organism) : Streptococcus pneumoniae
NCBI Protein GI : AEV46781
Other Database IDs : CDD:303028
Taxonomy ID : 1313
Protein Name : PspK
Protein pI : 9.72
Protein Weight : 64305.36
Protein Length : 631
Protein Note : Uncharacterized protein conserved in bacteria (DUF2213); cl19842

Protein Sequence : Show Sequence

>AEV46781.1 PspK [Streptococcus pneumoniae]
MNNKNIIPMSLASVAILATSFVANQPVTVKAEMASTASASSAVEQAKSKVAEAEKDVSKGQEIYDEAQKK
VQEKADVIRKIDEERQIINGEVQKAYLELRQAQEEKNKYPGRQEYTKKFDEANKKVDEITKKQKAKDEEY
TKKMYESIPDVGPLLTKLKAEKDKLAAAKRQLEKAKVAEKTEKEAKVKKLETELDQAREKVKKQAEEDKR
NYPTNTLKTLELEIAESYVKVKEAELELAKEEARELQDEDKFKQAKEKVESEKAEAKRLEELKTDRKKAA
EEAKRKAEEEAKRKAEEAKQKAEEAKRKAEEAKQKAEEEAKRKAEEEAKRKAEEEAKRKAEEEAKRKAEE
AKRKAEEEAKRKAEEEAKRKAEEEAKRKAEEAKRKAEEEAKRKAEEEAKRKAEEEAKRKAEEEAKRKAEE
EAKRKAEEEAKRKAEEAKRKAEEEAKRKAEEEAKRKAEEEAKRKAEEAKRKAEEEAKRKAEEEAKRKAEE
EAKRKAEEEAKRKAEEEAKRKAEEAKRKRVDSPVFAGTSLDNTYQAPAAKKGKQELPSTGAKDNVALASL
GFLGLFLGALPFVKHKN

Molecule Role : Protective antigen
Molecule Role Annotation : neutralization with purified recombinant PspK-R3 or rabbit anti-UD:R3 IgG inhibited binding of NESp to lung epithelial cells in vitro.(Jang et al., 2017)

25. psrP

Gene Name : psrP
Sequence Strain (Species/Organism) : Streptococcus pneumoniae ATCC 700669
VO ID : VO_0011197
NCBI Gene ID : 7329552
NCBI Protein GI : 221232510
Locus Tag : SPN23F_17820
Genbank Accession : FM211187
Protein Accession : YP_002511663
Taxonomy ID : 561276
Gene Starting Position : 1721442
Gene Ending Position : 1734743
Gene Strand (Orientation) : -
Protein Name : cell wall surface anchored protein
Protein pI : 3.83
Protein Weight : 378825.55
Protein Length : 4433

DNA Sequence : Show Sequence

>NC_011900.1:1721442-1734743 Streptococcus pneumoniae ATCC 700669 complete genome
CTTACTCTTCTTCATCACGTTTACGGCGTTTCGCAACCAATCCAATACCTGTAACAGCTGCTAGAACTCC
AAGTAACACAGATCCAATTGACGACTCAGTACCTGTATTAGGCAATTCTTTTTGGGATTTACCTGTCGAT
CCAGAAGTATTTCCAACTTGTGAGTTCGAATGGTTTGCTGAATTTGAAACTGATGTACTTGCTGAAGCTG
AGGCGCTTGTGCTTGCTAAAGCTGAGGCTGAGGCACTTGTTGATGCGGATTCAGATGCTGATGTGCTTGC
AGATACTGAAGCCGAAGCACTCGTTGACGCTGAAGCTGAAGCACTTGTTGAGGCTGATGCTGAGGCGCTA
GTACTTGCTGAGGCTGAAGCACTTGTTGATGCTGAAGCTGACGCACTCGTTGAGGCTGAGGCTGAAGCAC
TTGTTGATGCGGATGCTGACGCACTGGTTGATGCCGATTCAGAGGCACTGGTTGATGCGGATTCAGACGC
ACTTGTGCTTGCTGAGGCCGAGGCACTGGTTGATGCGGATTCAGAGGCACTGGTACTTGCTGACTCTGAT
GCACTTGTACTTGCTGAGGCTGAGGCGCTGGTTGACGCTGAAGCCGACGCACTCGTTGATGCGGAAGCTG
ACGCACTGGTGCTTGCTGAAGCCGAGGCACTGGTTGATGCGGATTCAGAGGCACTGGTTGACGCTGAGAC
TGAAGCACTTGTTGATGCTGAGGCCGAGGCGCTGGTTGACGCTGAAGCTGATGCACTCGTTGATGCTGAC
TCAGACGCACTGGTTGACGCTGATTCAGATGCTGAGGTGCTTGCTGAAGCTGACGCACTGGTTGACGCTG
AGACTGAAGCACTCGTTGAGGCCGATTCAGACGCTGAGGTGCTTGCTGAAGCTGATGCACTGGTACTTGC
TGAGGCCGAGGCACTCGTTGATGCTGAAGCTGACGCACTGGTTGACGCTGAAACTGAAGCACTCGTTGAG
GCCGATTCAGACGCTGAGGTGCTTGCTGAAGCTGATGCACTCGTTGAGGCCGATTCAGAAGCTGAGGTAC
TTGCTGAAGCTGAAGCACTCGTTGATGCTGATTCAGATGCTGAGGTGCTTGCTGAGGCTGACGCACTTGT
TGATGCCGATTCAGAGGCACTTGTCGACGCTGAAGCCGAAGCACTGGTTGATGCTGAGGCTGACGCACTT
GTACTTGCTGAAGCTGAGGCACTTGTCGATGCTGAGGCTGAGGCACTCGTTGACGCTGAAGCTGAGGCGC
TGGTACTTGCTGAAGCTGACGCACTGGTTGACGCTGAAGCCGACGCACTCGTTGATGCTGAGGCCGAGGC
GCTGGTTGACGCTGAAGCTGAGGCACTCGTTGATGCTGACTCAGACGCACTGGTTGACGCTGATTCAGAT
GCTGAGGTGCTTGCTGAGGCTGACGCACTGGTTGATGCCGATTCAGAGGCACTTGTCGACGCTGAAGCCG
AAGCACTGGTTGATGCTGAGGCTGACGCACTTGTACTTGCTGAAGCTGACGTACTTGCACTTGTTGATGC
GGATTCAGAAGCTGATGTGCTTGCTGAGGCCGACGCACTGGTTGACGCTGAAGCTGACGCACTCGTTGAT
GCTGAGGCTGAGGCAGACGTTGATGCAGATTCAGACGCTGAGGTGCTTGCTGAAGCCGACGCACTCGTTG
AGGCTGAGGCACTTGTACTTGCTGAAGCTGAGGCACTCGTTGATGCTGAGACTGACGCACTCGTTGATGC
GGAAGCTGAGGCACTTGTTGATGCTGAGGCTGACGCACTTGTACTTGCTGAAGCTGACGTACTTGCACTT
GTTGATGCGGATTCAGAAGCTGATGTGCTTGCTGAGGCCGACGCACTGGTTGACGCTGAAGCTGACGCAC
TCGTTGATGCGGAAGCTGACGCACTCGTTGATGCTGAGGCTGACGCACTTGTACTTGCTGAAGCTGATGC
ACTTGTGCTTGCTGAGGCTGACGCACTTGTACTTGCTGAAGCTGATGCACTTGTGCTTGCTGAGGCTGAC
GCACTGGTTGACGCTGACTCAGAAGCTGATGTGCTTGCTGAGGCTGATGCACTTGTTGATGCTGAAGCCG
ATGCACTTGTTGATGCTGAAGCTGAGGCGCTTGTGCTTGCTGAAGCCGAAGCACTGGTGCTTGCTGAAGC
CGATGCACTCGTTGATGCGGAAGCTGATGCACTAGTACTTGCTGAGGCTGACGCACTGGTTGACGCTGAA
GCTGAGGCGCTTGTGCTTGCTGAAGCCGAAGCACTGGTGCTTGCTGAAGCCGATGCACTCGTTGATGCGG
AAGCTGACGCACTGGTACTTGCTGACTCTGATGCACTGGTTGACGCTGAAGCCGATGCACTCGTTGATGC
TGAAGCCGACGCACTGGTGCTTGCTGAAGCCGATGCACTTGTTGATGCGGATTCAGAGGCACTTGTTGAT
GCTGAAGCCGATGCACTTGTTGATGCTGAAGCTGAGGCGCTTGTGCTTGCTGAAGCCGAAGCACTGGTGC
TTGCTGAAGCCGAAGCACTCGTTGATGCTGAAGCTGACGCACTCGTTGATGCTGACTCAGACGCACTCGT
TGACGCTGAAGCCGAAGCACTTGTACTTGCTGAGGCGCTAGTACTTGCTGAAGCTGATGCACTCGTTGAT
GCTGAAGCTGATGCACTAGTACTTGCTGAGGCTGACGCACTGGTTGATGCTGAAGCTGACGCACTTGTAC
TTGCTGAGGCTGAGGCAGACGTTGATGCGGATTCAGAAGCTGATGTGCTTGCTGAAGCTGAAGCACTTGT
CGATGCTGAAGCTGAGGCGCTGGTACTTGCTGAGGCTGAGGCACTGGTTAATGCTGAAGCTGATGTGCTT
GCTGAAGCTGACGCACTGGTTGACGCTGACTCAGAGGCGCTTGTGCTTGCTGAAGCTGACGCACTGGTAC
TTGCTGAGGCTGAGGCGCTGGTTGACGCTGAAGCTGAAGCACTTGTTGATGCGGAAGCTGATGCACTAGT
ACTTGCTGAAGCTGACGCACTGGTACTTGCTGAGGCTGAGGCAGACGTTGATGCGGATTCAGACGCACTG
GTGCTTGCTGAGGCCGAGGCACTGGTTGACGCTGAGACTGAAGCACTTGTTGATGCTGAGGCTGAGGCGC
TAGTACTTGCTGAAGCCGAAGCACTGGTGCTTGCTGAAGCCGAAGCACTCGTTGATGCTGAAGCTGATGC
ACTAGTACTTGCTGAAGCCGATGCACTGGTTGATGCGGATTCAGAGGCACTGGTTGACGCTGAGACTGAA
GCACTTGTTGATGCTGAGGCTGAGGCGCTAGTACTTGCTGAAGCCGAAGCACTGGTGCTTGCTGAGGCTG
ACGCACTGGTTGACGCTGAAGCCGACGCACTGGTTGACGCTGAAGCTGAAGCACTTGTCGATGCTGAAGC
CGACGCACTGGTGCTTGCTGAAGCTGACGCACTTGTTGATGCGGAAGCCGAAGCACTCGTTGACGCTGAA
GCTGACGCACTGGTACTTGCTGAAGCCGAAGCACTCGTTGATGCGGATTCAGACGCACTTGTTGATGCTG
AAGCCGATGCACTTGTTGATGCTGAGGCTGAGGCACTCGTTGATGCGGATTCAGATGCTGATGTGCTTGC
AGATACTGAAGCCGAAGCACTCGTTGACGCTGAAGCTGATGCACTAGTACTTGTTGACGCCGATGTGCTT
GCACTTGTCGATGCTGACGTGCTTGCCGATGTACTTGCGCTTGTTGATGCTGACGTGCTTGCTGAAGCCG
AAGCACTGGTACTTGCTGAAGCCGAAGCACTGGTTGATGCGGAAGCTGAGGCGCTCGTTGATGCCGATTC
AGACGCTGAGGTGCTTGCTGAGGCTGACGCGCTAGTGCTTGCTGAAGCCGACGCACTGGTTGATGCTGAA
GCTGAAGCGCTTGTCGATGCTGAGGCTGAGGCGCTGGTTGACGCTGAAGCTGAAGCACTGGTTGATGCGG
ATTCAGATGCTGATGTGCTTGCCGAAGCTGACGCACTTGTCGATGCTGAGGCTGACGCACTTGTACTTGC
TGAAGCTGATGCACTTGTGCTTGCTGAGGCTGACGCACTGGTTGATGCCGATTCAGAGGCACTTGTCGAC
GCTGAAGCCGAAGCACTGGTTGATGCTGAGGCTGAAGCGCTTGTCGATGCTGAAGCCGAAGCACTCGTTG
ATGCTGAAGCCGAGGCACTTGTCGATGCTGAGGCTGAGGCAGACGTTGATGCTGAAGCCGAGGCGCTGGT
TGACGCGGATGCTGAGGCGCTGGTGCTTGCTGAAGCCGACGCACTGGTTGACGCTGATTCAGATGCTGAT
GTGCTTGCTGAAGCCGAGGCACTGGTTGACGCTGAAGCCGACGCACTTATTGATGCAGATTCAGAAGCTG
ATGTACTTGCTGAAGCCGAGGCACTGGTTGACGCTGAAGCTGACGCACTGGTTGACGCTGAGGCTGAGGC
GCTAGTACTTGCTGATGCTGAAGCACTTGTTGATGCTGAAGCCGACGCACTGGTTGATGCGGAAGCTGAC
GCACTCGTTGATGCGGAAGCCGACGCACTCGTTGATGCGGAAGCTGAAGCACTTGTTGATGCGGAAGCTG
ACGCACTGGTACTTGCTGAAGCCGAAGCACTCGTTGATGCTGAAGCTGATGCACTGGTTGATGCCGATTC
AGACGCTGAGGTACTGGTACTTGCTGAGGCTGAGGCGCTGGTTGACGCTGAGGCTGACGCACTCGTTGAT
GCCGATTCAGACGCTGAGATACTTGCTGAGGCTGATGCACTGGTTGATGCTGAGGCTGAGGCGCTAGTAC
TTGCTGACTCTGATGCACTGGTTAATGCTGAAGCTGATGTGCTTGCTGAAGCTGACGCACTGGTTGACGC
TGACTCAGAGGCGCTTGTGCTTGCTGAAGCTGACGCACTGGTACTTGCTGAGGCTGAGGCGCTGGTTGAC
GCTGAAGCTGAAGCACTTGTTGATGCGGAAGCTGATGCACTAGTACTTGCTGAAGCTGACGCACTGGTAC
TTGCTGAGGCTGAGGCAGACGTTGATGCGGATTCAGACGCACTGGTGCTTGCTGAGGCCGAGGCACTGGT
TGACGCTGAGACTGAAGCACTTGTTGATGCTGAGGCTGAGGCGCTAGTACTTGCTGAAGCCGAAGCACTG
GTGCTTGCTGAAGCCGAAGCACTCGTTGATGCTGAAGCTGATGCACTAGTACTTGCTGAAGCCGATGCAC
TGGTTGATGCGGATTCAGAGGCACTGGTTGACGCTGAGACTGAAGCACTTGTTGATGCTGAGGCTGAGGC
GCTAGTACTTGCTGAAGCCGATGCACTTGTTGATGCTGAGGCTGAGGCACTCGTTGATGCGGATTCAGAT
GCTGATGTGCTTGCAGATACTGAAGCCGAAGCACTCGTTGACGCTGAAGCTGATGCACTAGTACTTGTTG
ACGCCGATGTGCTTGCACTTGTCGATGCTGACGTGCTTGCCGATGTACTTGCGCTTGTTGATGCTGACGT
GCTTGCTGAAGCCGAAGCACTGGTACTTGCTGAAGCCGAAGCACTGGTTGATGCGGAAGCTGAGGCGCTC
GTTGATGCCGATTCAGACGCTGAGGTGCTTGCTGAGGCTGACGCGCTAGTGCTTGCTGAAGCCGACGCAC
TGGTTGATGCTGAAGCTGAAGCGCTTGTCGATGCTGAGGCTGAGGCGCTGGTTGACGCTGAAGCTGAAGC
ACTGGTTGATGCGGATTCAGATGCTGATGTGCTTGCCGAAGCTGACGCACTTGTCGATGCTGAGGCTGAC
GCACTTGTACTTGCTGAAGCTGATGCACTTGTGCTTGCTGAGGCTGACGCACTGGTTGATGCCGATTCAG
AGGCACTTGTCGACGCTGAAGCCGAAGCACTGGTTGATGCTGAGGCTGAAGCGCTTGTCGATGCTGAAGC
CGAAGCACTCGTTGATGCTGAAGCCGAGGCACTTGTCGATGCTGAGGCTGAGGCAGACGTTGATGCTGAA
GCCGAGGCGCTGGTTGACGCGGATGCTGAGGCGCTGGTGCTTGCTGAAGCCGACGCACTGGTTGACGCTG
ATTCAGATGCTGATGTGCTTGCTGAAGCCGAGGCACTGGTTGACGCTGAAGCCGACGCACTTATTGATGC
AGATTCAGAAGCTGATGTACTTGCTGAAGCCGAGGCACTGGTTGACGCTGAAGCTGACGCACTGGTTGAC
GCTGAGGCTGAGGCGCTAGTACTTGCTGATGCTGAAGCACTTGTTGATGCTGAAGCCGACGCACTGGTTG
ATGCGGAAGCTGACGCACTCGTTGATGCGGAAGCCGACGCACTCGTTGATGCGGAAGCTGAAGCACTTGT
TGATGCGGAAGCTGACGCACTGGTACTTGCTGAAGCCGAAGCACTCGTTGATGCTGAAGCTGATGCACTG
GTTGATGCCGATTCAGACGCTGAGGTACTGGTACTTGCTGAGGCTGAGGCGCTGGTTGACGCTGAGGCTG
ACGCACTCGTTGATGCCGATTCAGACGCTGAGATACTTGCTGAGGCTGATGCACTGGTTGATGCTGAGGC
TGAGGCGCTAGTACTTGCTGACTCTGATGCACTGGTTAATGCTGAAGCTGATGTGCTTGCTGAAGCTGAC
GCACTGGTTGACGCTGACTCAGAGGCGCTTGTGCTTGCTGAAGCTGACGCACTGGTACTTGCTGAGGCTG
AGGCGCTGGTTGACGCTGAAGCTGAAGCACTTGTTGATGCGGAAGCTGATGCACTAGTACTTGCTGAAGC
TGACGCACTGGTACTTGCTGAGGCTGAGGCAGACGTTGATGCGGATTCAGACGCACTGGTGCTTGCTGAA
GCCGAAGCACTCGTTGATGCTGAAGCTGATGCACTAGTACTTGCTGAAGCCGATGCACTGGTTGATGCGG
ATTCAGAGGCACTGGTTGACGCTGAGACTGAAGCACTTGTTGATGCTGAGGCTGAGGCGCTAGTACTTGC
TGAAGCCGAAGCACTGGTGCTTGCTGAGGCTGACGCACTGGTTGACGCTGAAGCCGACGCACTGGTTGAC
GCTGAAGCTGAAGCACTTGTCGATGCTGAAGCCGACGCACTGGTGCTTGCTGAAGCTGACGCACTTGTTG
ATGCGGAAGCCGAAGCACTCGTTGACGCTGAAGCTGACGCACTGGTACTTGCTGAAGCCGAAGCACTCGT
TGATGCGGATTCAGACGCACTTGTTGATGCTGAAGCCGATGCACTTGTTGATGCTGAGGCTGAGGCACTC
GTTGATGCGGATTCAGATGCTGATGTGCTTGCAGATACTGAAGCCGAAGCACTCGTTGACGCTGAAGCTG
ATGCACTAGTACTTGTTGACGCCGATGTGCTTGCACTTGTCGATGCTGACGTGCTTGCCGATGTACTTGC
GCTTGTTGATGCTGACGTGCTTGCTGAAGCCGAAGCACTGGTACTTGCTGAAGCCGAAGCACTGGTTGAT
GCGGAAGCTGAGGCGCTCGTTGATGCCGATTCAGACGCTGAGGTGCTTGCTGAGGCTGACGCGCTAGTGC
TTGCTGAAGCCGACGCACTGGTTGATGCTGAAGCTGAAGCGCTTGTCGATGCTGAGGCTGAGGCGCTGGT
TGACGCTGAAGCTGAAGCACTGGTTGATGCGGATTCAGATGCTGATGTGCTTGCCGAAGCTGACGCACTT
GTCGATGCTGAGGCTGACGCACTTGTACTTGCTGAAGCTGATGCACTTGTGCTTGCTGAGGCTGACGCAC
TGGTTGATGCCGATTCAGAGGCACTTGTCGACGCTGAAGCCGAAGCACTGGTTGATGCTGAGGCTGAAGC
GCTTGTCGATGCTGAAGCCGAAGCACTCGTTGATGCTGAAGCCGAGGCACTTGTCGATGCTGAGGCTGAG
GCAGACGTTGATGCTGAAGCCGAGGCGCTGGTTGACGCGGATGCTGAGGCGCTGGTGCTTGCTGAAGCCG
ACGCACTGGTTGACGCTGATTCAGATGCTGATGTGCTTGCTGAAGCCGAGGCACTGGTTGACGCTGAAGC
CGACGCACTTATTGATGCAGATTCAGAAGCTGATGTACTTGCTGAAGCCGAGGCACTGGTTGACGCTGAA
GCTGACGCACTGGTTGACGCTGAGGCTGAGGCGCTAGTACTTGCTGATGCTGAAGCACTTGTTGATGCTG
AAGCCGACGCACTGGTTGATGCGGAAGCTGACGCACTCGTTGATGCGGAAGCCGACGCACTCGTTGATGC
GGAAGCTGAAGCACTTGTTGATGCGGAAGCTGACGCACTGGTACTTGCTGAAGCCGAAGCACTCGTTGAT
GCTGAAGCTGATGCACTGGTTGATGCCGATTCAGACGCTGAGGTACTGGTACTTGCTGAGGCTGAGGCGC
TGGTTGACGCTGAGGCTGACGCACTCGTTGATGCCGATTCAGACGCTGAGATACTTGCTGAGGCTGATGC
ACTGGTTGATGCTGAGGCTGAGGCGCTAGTACTTGCTGACTCTGATGCACTGGTTGATGCGGATTCAGAG
GCACTGGTTGACGCTGAGACTGAAGCACTCGTTGATGCTGAAGCCGACGCACTGGTACTTGCTGAAGCTG
AAGCACTTGTTGATGCTGAGGCTGAGGCGCTAGTACTTGCTGAAGCCGAAGCACTGGTGCTTGCTGAAGC
TGACGCACTTGTTGATGCGGAAGCCGAAGCACTCGTTGACGCTGAAGCTGACGCACTGGTACTTGCTGAA
GCCGAAGCACTCGTTGATGCGGATTCAGACGCACTTGTTGATGCTGAAGCCGATGCACTTGTTGATGCTG
AGGCTGAGGCACTCGTTGATGCGGATTCAGATGCTGATGTGCTTGCAGATACTGAAGCCGAAGCACTCGT
TGACGCTGAAGCTGATGCACTAGTACTTGTTGACGCCGATGTGCTTGCACTTGTCGATGCTGACGTGCTT
GCCGATGTACTTGCGCTTGTTGATGCTGACGTGCTTGCTGAAGCCGAAGCACTGGTACTTGCTGAAGCCG
AAGCACTGGTTGATGCGGAAGCTGAGGCGCTCGTTGATGCCGATTCAGACGCTGAGGTGCTTGCTGAGGC
TGACGCGCTAGTGCTTGCTGAAGCCGACGCACTGGTTGATGCTGAAGCTGAAGCGCTTGTCGATGCTGAG
GCTGAGGCGCTGGTTGACGCTGAAGCTGAAGCACTGGTTGATGCGGATTCAGATGCTGATGTGCTTGCCG
AAGCTGACGCACTTGTCGATGCTGAGGCTGACGCACTTGTACTTGCTGAAGCTGATGCACTTGTGCTTGC
TGAGGCTGACGCACTGGTTGATGCCGATTCAGAGGCACTTGTCGACGCTGAAGCCGAAGCACTGGTTGAT
GCTGAGGCTGAAGCGCTTGTCGATGCTGAAGCCGAAGCACTCGTTGATGCTGAAGCCGAGGCACTTGTCG
ATGCTGAGGCTGAGGCAGACGTTGATGCTGAAGCCGAGGCGCTGGTTGACGCGGATGCTGAGGCGCTGGT
GCTTGCTGAAGCCGACGCACTGGTTGACGCTGATTCAGATGCTGATGTGCTTGCTGAAGCCGAGGCACTG
GTTGACGCTGAAGCCGACGCACTTATTGATGCAGATTCAGAAGCTGATGTACTTGCTGAAGCCGAGGCAC
TGGTTGACGCTGAAGCTGACGCACTGGTTGACGCTGAGGCTGAGGCGCTAGTACTTGCTGATGCTGAAGC
ACTTGTTGATGCTGAAGCCGACGCACTGGTTGATGCGGAAGCTGACGCACTCGTTGATGCGGAAGCCGAC
GCACTCGTTGATGCGGAAGCTGAAGCACTTGTTGATGCGGAAGCTGACGCACTGGTACTTGCTGAAGCCG
AAGCACTCGTTGATGCTGAAGCTGATGCACTGGTTGATGCCGATTCAGACGCTGAGGTACTGGTACTTGC
TGAGGCTGAGGCGCTGGTTGACGCTGAGGCTGACGCACTCGTTGATGCCGATTCAGACGCTGAGATACTT
GCTGAGGCTGATGCACTGGTTGATGCTGAGGCTGAGGCGCTAGTACTTGCTGACTCTGATGCACTGGTTG
ATGCGGATTCAGAGGCACTGGTTGACGCTGAGACTGAAGCACTCGTTGATGCTGAAGCCGACGCACTGGT
ACTTGCTGAAGCTGAAGCACTTGTTGATGCTGAAGCTGATGCACTCGTTGATGCTGAAGCCGAGGCACTT
GTTGATGCTGATTCAGACGCTGAGATACTTGCTGAGGCTGATGCACTGGTTGATGCTGAGGCTGAGGCGC
TGGTTGATGCCGATTCAGACGCTGAGATACTTGCTGAGGCTGAGGCGCTTGTACTTGCTGAAGTCGACGC
ACTGGTTGAGGCCGATTCAGATGCTGACGTACTTGCACTTGTTGACGCCGATGTGCTTGCACTTGTCGAT
GCTGACGTGCTTGCCGATGTACTTGTGCTTGTTGATGCTGACGTGCTTGCTGAAGCCGAAGCACTGGTGC
TTGCTGAGGCTGACGCACTCGTTGATGCTGAAGCTGAAGCACTCGTTGATGCCGATTCAGACGCTGAGGT
ACTTGCTGAAGCCGACGCTGACGCACTCGTTGATGCTGAGGCTGAGGCACTTGTACTTGCTGATGCCGAA
GCACTCGTTGATGCGGAAGCCGAGGCACTGGTTGACGCTGAAGCCGACGCACTCGTTGATGCGGAAGCTG
ACGCACTGGTTGACGCTGAAGCTGAAGCACTGGTACTTGCTGATGCCGAAGCACTCGTTGATGCTGAGGC
TGAGGCGCTGGTACTTGCTGAGGCTGACGCACTCGTTGATGCGGATTCAGACGCACTCGTTGAGGCTGAT
TCAGATGCTGAGGTGCTTGCTGAAGCCGAAGCACTCGTTGATGCTGAGGCTGACGCACTGGTTGATGCTG
AAGCTGAAGCACTTGTACTTGCTGAGGCTGAGGCAGACGTTGATGCGGATTCAGAAGCTGATGTGCTTGC
TGAAGCTGACGCACTTGTCGATGCTGAAGCTGAGGCGCTGGTACTTGCTGAGGCTGATGCACTGGTTGAT
GCTGAAGCTGATGCACTTGTTGATGCTGAAGCTGACGCACTCGTTGACGCTGAAGCTGAGGCGCTTGTGC
TTGCTGAAGCCGAAGCACTGGTGCTTGCTGAAGCCGATGCACTCGTTGATGCGGAAGCTGACGCACTGGT
ACTTGCTGACTCTGATGCACTGGTTGACGCTGAAGCCGATGCACTCGTTGATGCTGAAGCCGACGCACTG
GTGCTTGCTGAAGCCGATGCACTTGTTGATGCGGATTCAGAGGCACTTGTTGATGCTGAAGCCGATGTAC
TTGTTGATGCTGAAGCTGAGGCGCTTGTGCTTGCTGAAGCCGAAGCACTGGTGCTTGCTGAAGCCGAAGC
ACTCGTTGATGCTGAAGCTGACGCACTCGTTGATGCTGACTCAGACGCACTCGTTGACGCTGAAGCCGAA
GCACTTGTACTTGCTGAGGCGCTAGTACTTGCTGAAGCTGATGCACTCGTTGATGCTGAAGCTGATGCAC
TAGTACTTGCTGAAGCTGAAGCACTCGTTGATGCAGATTCAGACGCTGAGGTGCTTGCTGAGGCTGAAGC
ACTTGTTGACGCTGAAGCCGACGCACTGGTTGATGCTGAAGCTGACGCACTGGTACTTGCTGAGGCTGAG
GCGCTGGTTGACGCTGAAGCTGACGCACTGGTACTTGCTGACTCTGATGCACTGGTTGACGCTGAAGCCG
AAGCACTCGTTGATGCTGAAGCTGATGCACTAGTACTTGCTGAGACTGACGCACTCGTTGATGCCGATTC
AGACGCTGAGGTACTTGCTGAAGCCGAAGCACTCGTTGATGCCGATGTACTTGCTGACGTACTTGCGCTT
GTTGATGCGGACGTGCTTGCTGACGTACTTGCGCTTGTTGATGCGGACGTACTTGCTGACGTACTTGCGC
TTGTTGATGCTGACGTGCTTGCTGACGTACTTGCGCTTGTTGATGCGGACGTACTTGCTGAGGTCGACGC
GCTTGTTGACGCCGATGTACTTGCTGACGTGCTTGCTGAAGCCGACGCACTCGTTGATGCCGATTCAGAC
GCTGAGGCGCTTTGACTAGCACTTACTGAGAGTGACTTAGACTGTGAAAGTGACTGACTGGTCGTGCTAG
ATTCAACTATCTTTCCTTTACTATTGAAGTAGTTAGTTCTAATTTTATCTGTTTTAGCAGCGTAAGGGGT
AAATGTAAAGGATGTATCCGTTCCAGTAATTGGTACAGTCCAAGATGATGTTAATCCATATCCTCGCTTA
GCAAATAAACCATTCATTTGGGCACCATTTCCCCAAGTATACCCACTACCCTGCGTCGTCATTGTAGACA
TATTATAGTATTGAACCTGTACACCATCTTTGTCAGTAATGTAGTTCTTTACACTTGAAGGTGTTTTCAA
TCCCTTTCCTAACGTTAACATCGTTTGATAAATTGTTCCTGTATTATAGATAGAATATCCACGAGACATT
CTTGAAAGATTTCCAAGAGTCTTTGTGATAGGATCTACATACGTAACCGTATAGGTAAAGGTCAATTTTG
AACCATCATTTGTCACTGTCAATTTATAGTAAATTGGAATATTATAAAATGAATCTCTACCTGTACCCGT
GTAAATTTTTGTCTCACTTTTAGCAATGTTTAGACTTGCATTAATTGCAGGGGCGCCATTTACAATCGTA
TTGCCAGAAAAAACAGCAGCATTTATAGAAGCCAGCAATTGCTCGATGGAATCTACTGAACGCTTGCGTC
GCTTAGCATAAGATTGGAGATTGACATTTGTAACTGATGCTACATAATCACTAGCTGGTTTCTTACGATC
TTCTTCGACCTTCTTAGCAGTTGCTTCTGTAGCGGCAGCTGTTTGTGAACCTACCACAGTAGATGATGCA
GAAATACTTGTCGAAGCCGATGTACTTGCTGATTCTGATGCACTTGTACTCGCTGAGGTCGAAGCGCTGG
TTGAGGCTGACTCAGATGCAGATGTACTTGCCGACTCTGAAGCTGACAAACTAGTTGAACTCGCTGAATT
ACTTGTAGATATCGTACCTAGAACTACTGTATCATTTGTTGCCAAAGCATCCGTTTTCTCTACAGTTTTT
TCAAGTACTGCCGACTCATTTGTAAATACCTTCGTTTGAGTTGCAACGGTTCCACTTATGACAGTTCCCG
CAGCAACTATCCCCTTGAGGATATCAAGCCCAGTAATTGAATGACTTACTTTATCTTCTACCGTTTCGGT
CA

Protein Sequence : Show Sequence

>YP_002511663.1 modified cell wall surface anchored protein contain S-SRR1-BR region[Streptococcus pneumoniae ATCC 700669]
MTETVEDKVSHSITGLDILKGIVAAGTVISGTVATQTKVFTNESAVLEKTVEKTDALATNDTVVLGTIST
SNSASSTSLSASESASTSASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVE
EDRKKPASDYVASVTNVNLQSYAKRRKRSVDSIEQLLASINAAVFSGNTIVNGAPAINASLNIAKSETKI
YTGTGRDSFYNIPIYYKLTVTNDGSKLTFTYTVTYVDPITKTLGNLSRMSRGYSIYNTGTIYQTMLTLGK
GLKTPSSVKNYITDKDGVQVQYYNMSTMTTQGSGYTWGNGAQMNGLFAKRGYGLTSSWTVP

Molecule Role : Protective antigen
Molecule Role Annotation : Mice immunized with recombinant (r)PsrP(BR) had significantly less bacteria in their blood and improved survival versus controls following intranasal challenge (Shivshankar et al., 2009).

26. SrtA

Gene Name : SrtA
Sequence Strain (Species/Organism) : Streptococcus pneumoniae D39
VO ID : VO_0011199
NCBI Gene ID : 4442999
NCBI Protein GI : 116515518
Locus Tag : SPD_1076
Genbank Accession : CP000410
Protein Accession : YP_816547
Taxonomy ID : 373153
Gene Starting Position : 1102250
Gene Ending Position : 1102993
Gene Strand (Orientation) : -
Protein Name : sortase
Protein pI : 5.46
Protein Weight : 26445.89
Protein Length : 247
Protein Note : equivalent gene in S.pneumoniae TIGR4 = SP1218; equivalent gene in S.pneumoniae R6 = spr1098; identified by match to protein family HMM PF04203; match to protein family HMM TIGR01076

DNA Sequence : Show Sequence

>NC_008533.1:1102250-1102993 Streptococcus pneumoniae D39, complete genome
ATTAATAAAATTGTTTATATGGTTGATTGAAAGCTGTTAGGATTTCATCAGATGTTTGTGAATAATCTTT
TGTTTCTTTCAAATCACCTTTGACAATAATACGTTCTGTAGCAGCAAGGTCTTCACAGGTTACTAATGTG
ATTTCATTGACCCCATCTCTATCATCAACTTCATCAACACGATCTGGTGTCACACGTTTGACTTCACGTA
TTTCATAAGTATAAACTTTATTTTTATCGGTTAGATAAATCTTCATGCCATTTTTAGCATTATCTAAAGG
AGAAAATAACATTTTATTAGCATTATCAACACCAAAGATATGGTGACTAGCTAGACTATAATTTCCTTCT
CCCATTACTTGCTCGCGTTTCATTGTACCAGCTCCGTAGAAGAGATTAACATTATCAAGTCCTTTAAAAA
TCGGCAAATTCATTTCCAATTCAGGAATTGCAATTCCCCCAATAACTGGTAATTTTTGAGCATCCCATTG
AGAAGTTAGAACAGCTTCCGAAGAGATAGCTTTGACAGAATCAAAGTCAAAATTGCCTTCTGTATCCTGA
TTTTCTTCTAATTTTTCTTTTGATACCTGGCTAACTTGATACTTATTGGTATTCCAGACTATGAAAATAT
TTCGAATTTGAGTATTAAAAATCAAAGCCAGTGACAGTAATATCAGAAATCCTGCTAGGATATTTGTCAG
CAGATTTTTTCGCTTGTTTTTCTTTTTATTATTTTTTTGAGACA

Protein Sequence : Show Sequence

>YP_816547.1 sortase [Streptococcus pneumoniae D39]
MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVWNTNKYQVSQVSKEKLEENQDTEGNFD
FDSVKAISSEAVLTSQWDAQKLPVIGGIAIPELEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLAS
HHIFGVDNANKMLFSPLDNAKNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDL
AATERIIVKGDLKETKDYSQTSDEILTAFNQPYKQFY

Molecule Role : Protective antigen
Molecule Role Annotation : Intraperitoneal immunization with recombinant SrtA was found to have conferred to mice protection against S. pneumoniae intraperitoneal challenge and the passive transfer of immune serum before intraperitoneal challenge was also protective. Moreover, by using the intranasal challenge model, significant reduction of bacteremia when mice were intraperitoneally immunized with SrtA was observed, while a moderate decrease of lung infection was achieved by intranasal immunization, even though no influence on nasopharynx colonization was seen (Gianfaldoni et al., 2009).
Related Vaccine(s): S. pneumoniae SrtA Protein Vaccine

III. Vaccine Related Host Genes

1. IgA

Gene Name : IgA
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 238447
Genbank Accession : AC160982
Taxonomy ID : 10090
Chromosome No : 12
Gene Starting Position : 8607
Gene Ending Position : 12639
Gene Strand (Orientation) : -
Protein Name : immunoglobulin heavy constant alpha
Protein Note : Also known as IgA; Igh-2

DNA Sequence : Show Sequence

>gi|121699722:8607-12639 Mus musculus immunoglobulin heavy chain complex (Igh) on chromosome 12
AGTACTGGGGGACCTCTTTGCTGCCAAACGGGCCTCGAACAGTTGTAACAGTGAGTGCTGTGCTGTAGAA
CAAGCTCAGTAGGAAGAGGGTGAGGAAGGTCACAGTGGTGGGCCACAGGCTGGCACCTGGGGCCTCTTCC
TCCAGGATGTCTTCTGACTGGTCCAGTAGCACATAGGAAAGTGGCTCTTGACGTTCTGTAAGACATAGAT
GACCTTCTAGATCCAAACCCATGGGGATGGGGGCTCAGACTTAAGGTTTCTGCTTCTATGCCTCCTTGGG
CTTTGGGGACAGTGGTGGTAGGGGTCCCTTATTCCCGGGAAGGGACTTGTTCAGGGATAATGAGGAGGCA
GGGTCCCAGGTAAGGGACTGGAATCCCAGCAGTTCTTGAGATGGCCCCAAGACCCATCTCTCTCTCTTAG
GGAAACCTTAATTAGAGACCTGTCATGGTAAGAGAGTTGTTTCTGTCAAACTCTGAGAAGAGTTTGAACC
CAAGGCTCCTTAGGTCTCAGTCCTTTCTAGATATTTAGCAATACCTCCCTAAACCCCAGTTCTTAGGCTA
ATCTTGGCTTGGGTACTAGGGTTGTGTAAGATCAGACACCTTCAAACAGATACAGGCCAATGTGATTTCT
GGGCCAAAGTTAGACTACCAAGGCCTGGACATGTGGTCAGAGGACTCTGTTGCATTCTTAAGAGCTGTGA
CTGGGGGTTAAGCAACAGTAAAGACCCCCAGTAGAGCCAACCCTGCTGTCCTCAGGTTCTCTGCGCACAG
AGTTTCATTTTAAGTGGCAGTAGGAAATGTGTACTGATAGTCAACCACTCGTATTTCTGTCACTGCAGCC
ATCCATCAATCCATCCATCATCCCCATCGTTCATCCTTGTAGCCAGCCATCCATTACCCTCCAGCCGCTC
ACTCATCATCTTTCCCGACTATCGTGATTCTCCAGCTTCTCTGCCCCCTCTCCCTGTGGGGATTCAGTCT
TTCACCTATTGACTCCTCCTTACTAGGATTCTCTGTCATGGGCCAGGCCTTGGATTCCATGGGTGGCTAT
GGTACTTTTCTGAGTCAGGTTTCCCAAGCTGGTCTGTTGCTCTTGCTATTCTCAGGGAACTGGACTTTCA
AGCTGGAGAGTATCCAGGGCATGCATGGGCCTTTACTCCACTCTTGCTCTGAGAGCAGAGGGAAGTTGCT
AGAAACCAGCAACTCCTCCCCCCCCCCCCCGAAGTTCTGAAGGCCTGTGAGACAGGAGCAGGGCCCCCAC
TGCCCTCTTGTGGACACCTGGGCTCCAAGTATACCTGGTTGTAGAACTTTGTGTTCTAGGGGTGCCCATG
GAGGAGGCCGAGGCCTGTGTGGGAGTGGTCTCCATGGCCTGCTCCTGCTTCTTCCCAGATTTGCCTCTTA
TCCTGCTTCTGGTTTGTGATACTTGGGCTCAGGATGGGAAGGGAGACTTAGGTTAGAAAGGGGGCACCTG
GAGGGGCCTATGCCCTTGTCCATTGTGTGTAGTTGTCAGTGTCGGGCCTGGAAATTCTCCCGGGGGCTTG
GAGGGGACTCCCCTTGCTTTCCAGAAGCTGGGTGTGGGCTGTGAACTTCATCTTCCTCCAGGGTTCAGGT
CTGTGTGAGTGTGCAAATGTGAGTGTCTGTGCATGTATGCCCATGTGCATGTATGCAGAACAGGGTGTAT
GCCCATGTGCATGTGTGAAGAGCAGGGTGCTGCTGAGGGTTGTCTGTGCCATGCTATATGTCTTTCTCAT
GAGTCAGTGGTCACCCTGAATGCATGTTTCCATTCAGGGATGGCCTACTGAGGGTGCGTGGCATCTTCTT
CCCAGTGCCCCTGTGTTCATCCCTTCATTCTCATACCATCATCTCTATTGTGACCCCCACACGGCACCAG
CCTTGATGGTGCTACCCTTTTGCTCCATCTATCCCTGTCTTGTTTGGGTTAGAAGCTGCGGTCTAATAAA
CAGCCACACCACCCACCACTCAGAGTACTTGTGCAGCAACCCACTGCCCTGGGGACTCTCCCATGAGCTT
TGCCTTCTTGGCTGAGTCTGCTCGCCTTGGATTTCATCCCCAGTTTATTCTTACTTCCCATTCACTCATT
GCACATTCACAGGGCAGGTGCACACACTCTCATGTTTCCACCAACATGAACACACATAGATATATGCATG
CTCATAGGTATGCACAGGCACACACTTGCTCACAGGGACATAGATATAGTGTGTCCACATATGTACTTAT
ACCCATATACATACCTATAAACCATAGGTGCACACAAACTCACTCCTCTCTTGCTGCACACAGGTACACA
CATGAAGGCACATGGACCCATCCATAAGCACTCACCAATACATAGACATGCATACACATATACTCTAGGC
ATGAAGGGTCTTCAAAGTCTCCGAGGCCTTACAAGCTTAGGTGGTGGTGTTTCTCCCTCCCTGGGCCCTG
ACCCCTCCCTGTGTTCTGTAGACCCTAAGGATGATGGACAGGCACTGGATGGAAGTGCAGGGATACTTTG
GATGAGCACAGAGTTTATTTCAGGAGTAGGGACAGGCAGGGTGGCTCAGTAGCAGATGCCATCTCCCTCT
GACATGATCACAGACACGCTGACATTGGTGGGTTTACCCGACAGACGGTCGATGGTCTTCTGGGTGAAGT
TCATGGGCAAGGCCTCGTGGCCCACCATGCAGGAGTACTGGTCACCCTGTTTCCAGAGTTCAGCTGATAC
ACGCAACACGCTTGTCACCAGGTAGGTGGTGGCTCCCTCGCCTGGCTCCTTTAGGGGCTCAAACACTAGG
TAGCTTTCTGGGGACAGCTCCTCATTTCCATGCAGCCATCGCACCAGCACTTCTTTAGGGTTGAAAGCTC
GCACCAGGCATGTCAGGGACACGAGCTCATTCAGGGCCAGCTCCTCCGACGGCGGCGGTAGCAGGTGGAC
CTGGGGTGGGAAGGTGTTCACTGGAAGGTAGAAAGAAGAGTTATGGCTCAGACAAGGAGAGCAAGACCCC
TCTGCCCTTCCCTTCTGGGCAGTTCCTCAGATAGCTCCAATTTCCCTCTGTGATATAAGGACAGGAGCAG
TTAGTTGAGCATCTGTATTATAGGAAGAAAGCAGGCATGTGAGCAGGGAACGTCATACAATGTCCCGGGT
ATGCATCTGGGCTCACCTGTGATTTTGGCAATTGTGCCAGTTAAGGTGTCAGACTCAGGATGGGTAACTG
TGCACTTGAATGATGCGCCACTGTTCCAGCGCTCAGCACAGCCAGGCAGGACGCTGGACACACTGTAGCA
GCCGCAGGAATTCTGCACAGCTTTCTTCTGCACTGCATCCTTCCCAGTGGAGGGCTCCCAGGTGAAGACA
GCTCCCTCAGGATTTCTCAGGCCATTCAGAGTACATGTGAGGCTGGCATCTGAACCCAGGAGCAGGTCCT
CAAGAGCTGGCCGCTGCAGTGACAGGCTGGGATGGCAGGAAGGAGGACAAGGAGGACAAGGAGGAGGAGG
ACCTGTAACAGAGAAGTCCTAGCAAATCAGTATATTCTCCTTTTCTCCTTTGCTGTCCCCTTTTCTGGAT
GTTCTGAGGCCTCAGTTCCTTATGGCCTGTTTACGCTTCCCTTCCCCCACCCCCATGGGAGGTTTCTGAG
GGTCCCTGTGAGGGTGTGCCTCAGAGGGATATATGGATCTAGATAAGGTAGAACTTATCCCACCCCCAGC
TGACCCCCTAACGTTCTTTACCAGAGCACTTCACATCCAATTCTTGGACGGCGTTAGAGTCATGTTGCAC
GGAACATTTCACGGATTCTCCTTCTGGGCACTCGACAGCTGGCAGGGTCAACTGGCTGCTCATGGTGTAC
CCTCCCCCAGAGGCCAGGGCAGGTGGGAAGTTTACGGTGGTTATATCCTTCCCACTCTTTCCCCAGGTCA
CATTCATCGTGCCGGAAGGGAAGTAATCGTGAATCAGGCAGCCGATTATCACTGGGTCACTTGACAGAGC
TCGTGGGAGTGTCAGTGGGTAGATGGTGGGATTTCTCGCAGAC

Molecule Role : Vaximmutor
Related Vaccine(s): S. pneumoniae RASV synthesizing PsaA

2. IgG

Gene Name : IgG
Sequence Strain (Species/Organism) : Mus musculus
NCBI Gene ID : 16059
Genbank Accession : AF010213
Taxonomy ID : 10090
Chromosome No : 12
Gene Starting Position : 881411
Gene Ending Position : 914690
Gene Strand (Orientation) : +
Protein Name : immunoglobulin heavy chain (V7183 family)
Protein Note : Also known as IgG; IgH; VI24H; VH7183; B9-scFv; IgVH1(VSG)

DNA Sequence : Show Sequence

>gi|94393741:881411-914690 Mus musculus strain 129/SvJ chromosome 12 unlocalized genomic contig, MGSCv37 alternate locus group 129/SvJ
CATGGACTTTGGGCTCAGCTGTGTTTTCCTTGTCCTCATTTTAAGAGGTAATTTGTAGAAATAAGATCCT
GCCAGTATTGTGTACAGGAGAAATAGAAAAATTTTTCTTTCCTCTATTTTGTTTTGTTTTGTTAGTGACA
GTTTACAAATAAGCATTCTCTGTTGTGAGGTGCCCAGTGTGAGGTGAAGATGGTGGAGTCTGTGGGAGGC
TTAGTGCAGCCTGTAGGGTCATTGAAACCCTCCTGTGCAGCCTCTGGATTCATTCTCACTGACTACTGAA
TGACCTGGATCCTTCAGGCTTCAAAGAAAAGGATGGAGAGGGTGACAATAAAATTTTTCCCACTCACACC
CACTTGCTGGCCCTGGCGTTCCCCTGTAATGAGGCACATAAAGTTTGCAAAACCAAGGGGCCTCTCTTCT
CAATGATGGCTGACTAGGACATCTTCTGATACATATGCAGCTAGAGACATGAGCTCCCGGGATACTGGTT
ACTTCATATTGTTGTTCCCCCACAACAGTTGCAGACCCTTTTATTTCTGTGGGTACTTTCACTAGCTCCT
CCATTGGGGGCCCTGTGTTCCATCCAATAGCTGACTGTGAGCATCCACTTCTGTAGTTGCTAGGCCCCGG
CATAGCCTCACAAGAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTTGCTGGTGTATGCAATGGTGTC
AGAGTTTGGAGTCTGATTATGGGATGGATCCCCAGGTATGGCAAACTCTAGATGGTCCATCCTTTCCCAA
CAATAATTTTTAATGGTGGAGATAGCACCTACTATCCAGACACAATGAAGGGCCAATTCACTATCTCTAG
AGATGATGCCAAAAACACACTTTACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTATACA
TATATTCCTAAGCTTGAGTAAGTGGACTTAAACTGATTCCATCACAACTTGCATGAGATGGATATTCCCC
AGTGTTAAGACCTGTCACCATCACTGTCAATCAGAAGACAAAGTTTATGCACAACAAAACAAAAAAACCA
AAAGCAGAGGCCTCCAATTACAAGTAATAGACCCAGACCCACAGTCTCTGAAAACTGACTGTACAGTTGG
ATCCAGTCTTTTACTTCTTTTCCCTGGATTTTATATTACTGAGGAAATGAGGAAAGCTCTACAATATCTG
TTCTCCATAGTGCTCAACACCTCCAAGCACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTTCTT
GTCTTGTAGACTACTTCAACCTATTTTGTACTCCAGTTAATGAAACTCAAGTCTAGCAGCCTGTCACTGT
TTATTCTAAAGTATTATGAACAGGTGACCTCCCATCCTTCCCCAACGCAATAATCATATTTAGGAATTTG
AGGTTTTATGAGATATGATCTCAGGGTAGAGAGAGAAAGCAAACTACATAGAAATATAGACTGACATAAA
TCAAGACTTGCATAAGCTAGTCCCCAAGTTCCATGTCCCTAAGTGGCAAGGACTATCTTCTGAGCCTAAT
GAGATGAGATCCAAATCAAACCTCCTGGGTCTTTTTAGATAAACATGTGAGATCAATAGACTAAATGCTT
TGGCTGGGCTTCCTTGCAATCCAATTCCCAAACAAAAATGGATCTGGCTCCACAGACACCACAAGAATAG
TCTTAAATAGTTCTTTAAGTAGAATGTCTGATCACTACGAGCCCAATTCCATCCTAAATACTCTTCTGGA
TTATACATAAATAAAAATTGAACATAGGGCATGGGGCACTGATCTCCCTGTGCTACATGAATGGGGGCTC
ATTTACTAAATGTTCCCATTTTTCTTTCTAGCGCTGCACAGTGCAAATCCTACAACTTCCTGTTTATCTA
CAATGTGAACTCCAAACAGTACAAGAAAAAACGTTCCTTATGTTCCTCTCCAGGTTCTCCAACAAGCACA
GAGCAACCTTCTGTCACCAGGACGGAAAACTGGAAACCTTGCTCACTGCTTCCTTTTATTCCCTCGGGAA
CCTCCCCCAATGCAAAGCAGCCCTCAGGCAGAGGATAAAAGCTCACACAAAGACGAGAAGCCCCATCCTC
TTCTCATAGAGCATCCATCAGAGCATGGCTGTCCTGGGGCTGCTTCTCTGCCTGGTGACTTTCCCAAGCT
GTAAGTGTTTCAGGGTTTCAGAAGAGGGACTAAGACATGTCAGCTAGAAGATGTGTGACTAATGGTGATG
TTGCTTGTCCCCAGGTGTCCTGTCCCAGGTGCAGCTGAAGGAGTCAGGACCTGGCCTGGTGGCGCCCTCA
CAGAGCCTGTCCATCACATGCACCGTCTCAGGGTTCTCATTAACTAGCTATGGTGTACACTGGGTTCGCC
AGCCTCCAGGAAAGGGTCTGGAGTGGCTGGTAGTGATATGGAGTGATGGAAGCACAACCTATAATTCAGC
TCTCAAATCCAGACTGAGCATCAGCAAGGACAACTCCAAGAGCCAAGTTTTCTTAAAAATGAACAGTCTC
CAAACTGATGACACAGCCATGTACTACTGTGCCAGAAACACAGTGTGGGAAGTCCAATGTGAGCCTGCAC
AAATACTTCTCTGCAGGGATGATCACAACCAGCAGGGGGCGCTGATGACCCAAAGGGACTTCCCAGGATC
TCTTCTGGAATCTAGGGAGTTCTGGCCTGTGTCTATCAGCATGTGTTTCAATGTTAGAGTTGTGAGTTTT
CCTTCCAGCAACAGAGATATTTTAGAGCCCACTTTTCATGGTCATTCTACTAAATTTGTTCACATAGTGG
AAAGATTTGTTAAATGATTCTATAGTCTAATAGGGTCAAACAAAAACAAATAATTGAGTTCATATCTACC
AAAAAAAAAAAAATTCCCTCAAAGTGGACAACTGTGTAGGTGAGGAAAACCAGGGGGATTTGGGAATAGA
TTATTTCTCTCCCGACTGTGGTGTTAGTCACCTTTCAGCCATTTACTGTATTTAATTCATGTTTAAAGTT
AATTGTATTCATCTTCCCTAACATGAAGTTTTCAAACACATGTCCACAATTTAATGATGACACAGTTATC
ATTGTGAATGATAACTCATAAGATCTCTCTCCATATATGAAACACACGATATTCTGTTATTAGCTATAGC
CAACAAAGTTACATATCATTATTATTACTGAAATAATTCTTCCCATCTAACTGAATAGTTTTCTCACTCG
ACACTGCCTATGCTGCGATCAGCCTGCTCTAATTTTACCTTTGCTCAGAGCACTGCTTTCTGTTTCATTT
GATAAGATGGTGCCTGGTTGTCACTGAAAATGTGTCCTCCAGTTTCACAGTGATTAATGTGATTTTCAGT
ACATTGAGAACAGAGCCACAGCAAAAATGAGATGGAATGTCATCATTTTTACGCCAGGTGCTGCAGAAAT
AGCTAGGTGGTTAAGAGAGAATATTGAACTTTGAGGGGTTCAGATTAAATTCCTGATACCCACAATGTGC
ACCTCATAACCACATGTACATATAGAAAACTCAGTATAAATGTGGCCTCCGTGAGCACCACACTACTCCC
ACAAACACATATACACATAATTAAAAGTAAAGATTTTAGAAAAATGGCTAATCTAATGATAGGAAGTAGT
CAAAAAGAGAGTTCTTTTGTCATGTACATATGTGTAGCAGACTTAAATGTTAAACATTCAAGAATACATT
CCTCGATCCACATTAAAATTTTGCAAAGAGTAGCACAGACGGTGGCAATTGCTAAACTTATATCTAGAAA
CACAATTATGTGTGGTGATATTTAATTACACATTTATACCAGGACATATGACAATATGGAAACCAAACAT
GTTGTATCCACATGCTCTAAGGAAAACTAAATGGAGTGTGATAAAACCAAAGAAAATGTGAATATGAAAT
ATTTTTCCAACTCTGCATCTTAAAACGGTTTCTTTCATGTGTCATATCTGCTATGAGGACTTTCTTCTGC
CCATGTCCAACTCCAGAGCATGCCACAGCAGGAAGACCTACAGGTATTACTTCTCTGCACCCAGGAAAAC
CACCTCTGTCCTGACCCTGCAGCTCTCAGAAGAGCCCAGACCTTCATTCTCAGGCCCTCATCCAGTAATC
AGCACTGAATACAGAGCACTCACCATGGACTTTGGGCTCAGCTTGGTTTTCCTTGTCCTTATTTTAAAAG
GTAATTCATAGAGATAAGATTCTATCTGTTTTGTGTACATGAGAAACAGAAAAATTGTATTGTTTCTCTA
TTTTGTTTTGTTTTGTTAGTGACAGTTTCTGACTCAAGATTCTCTGTTTGAAGGTACCCAGTGTGAGGTG
AAGCTGGTGAAGTCTAAGGGGAGGCATAGTGCAGCCTAGAAGGTCCATGATACTCTACTGTGCAGCCTCG
GATTCACTGTAAGTGACGACTGGTTTGTCCGTGTTTGCCAGGCTCCAAAGAAGGGGCTGCAGTGGGGGAT
GGGAATAATTTTTCATGGTTGTGGTAGCCCCTCTTATGCAGACACCTTGAAGAAGTGGGTTGGACGTAAC
ATATTCAGAATCAATATTTAAAGATTCTAATCCTTGAAGATATCACTTTTGACCAAGTATATATGAACCA
TGTTACTGAGGTTTATGGAGGTTTGAGTATGTTAGGTCCATGGATAGGGAAAATATTAGGGGATTTTAGG
AGTAACTGAGGCTTGTTGTAGGAAGGACATCACTGTAGGGGTAGGCTCCGTGTTCCTATCTTCAAGCTCT
ACCCAGTGCAGAATAGAGCCCTCTTCTGCCGGCAAGTGGAAAGAGTTTCTCTTCCTGGCTGCCTTCAATC
CAAGTTGTAGAATATCAAATCTCCTAACACTATGACTGCGAGCATGCTGACATGCGTCCCACCATAAAAA
TAGACTGAACCTCTGAAGCTTTAAGCCAGCCTCTAGTAACTGTATTCTTTAATAAGACTGAACTTGGCTA
TGGTGTCTTTTCACAGTAAAATGGAAACATAGACAAGGTTCTAACTCTCTGTACTAGAGGACCATCTCTA
TGTCCTTGGTGTTGTATGTAAATTATTGAACAAATACTATAGACAAGTTGTATGGCCAAAAGTCCATCCC
CTTCAACAATCTACTATCTGGAACCATATAGATTAGATTCCTTTCCTGCACTCATTTCCCTTACTTGCTG
AGACATTTTGAAGACATGCTCAGAATCCCTGAACTTCCTGCTGAAAAAAAAATACCCCTCCAATTTGAAG
ACAGTTCTCTTCCAAAATTCATCATAAATACTGATCCACATGTCCAGGCATATCATGTAGTTTTAAAAAA
CAAACCAATCAATGCTAAAGTGGGTAAGTGCCTACATCATTGGTTTATGTCTGTTCATAACCTGGTATAT
AGCTAGAGACCAGGACTGCATGTTTCTCCTAGGCCACACCTCTCCCACAAATGCTGGCTCTGCCTCTCCA
GAATTCCAAGATCATGGATCTCTGGGTCCCCAAGGAACAATAAGTGTGCACTCTTGCCAGCCTGTACACT
ACTGCACTAGCTTCTCCCCCTGGAGCTTGGTCATTGCTCAATCTTCCCTTTCCAAAGCCTGGCCAAATTC
CTCCTACCTTTTGTTCTTTCAGTCAGGCCACCTAAGTCATCTTGAATGAAAAACAACACAAAATCTGAAC
TTAAAATCAACAAATAATACAAGTGCTGCGCACAAAATCAATCATTCTAAACTCATCAACTATTTTATGA
TGGAAATCTTCCAACATACAAGCTACCACCAGGTCTAAGAATTACTCATCTACATGTTGCTTCCTCTCAC
TCACAGCCTAACCATAAAAGGGCTGTTTCTTCCTCCCATGTCCCCTCTTTTGCCTTCAGAAGCAGAAGCT
CCACCTCCTCCTCTTTGCCCAGCAATTGGCTTCTTGTCGTCTTTATTATCATATTAATTACTTAGGGGAA
AATCCCGTGTAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGG
AAGGCTCACCAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAG
CCATAGGGGCTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACG
CCTTTCATCTGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAA
TATAAGGACATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTG
AGTAACTGCTAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGA
CTCTAAGTCATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTG
ACTAATACAGAAGTTGGTACCAGGAGTGGTTCTAGAGTAACAGAAGTACAAGGATGAATCTTTTAAAATA
CTGGAATTGGCTTGTTGATCCACCAGCACTTTCAACTATTGAAACCTCTCCAGATTCTCTCCCTCCTGGG
AGCTCAGAGAATTTTGAAGACCCATGGTTGAAACTATATTCCGAACTTAAAGAAGCAAATGCCCTTGATT
TTCTTAATGAATTAGGTGATTCAGTGCACAAAGCTTTCTACAAGATGGGGAAAAAATTGGAAAATGATTT
TACTGGCTGGCTGCTCTTAGTATCTGTGGAAAAAATGATGAATGAAAGGAAGGAGTTGTGTGATAAAATC
GAAAGGCTCCAGACACAAGTAAACGATCTAAAAGTTGCTAAGTGTGTCCTTGAGGAGAATCTTCTCTCTT
GTAGCAATAGAGCTCAAGTTGCAGAAAATCAAACAGAAACTCTCATTGTAAGGTTGGCTGAACTACAGCG
AAAATTCAAGTCTCAGCCTCAGAGTGTGTCAACAGTTAAAGTAAGGGCTCTAATTGGCAAAGAATGGGAT
CCTACAACATGGGACGGGGATGTGTGGGAAGACCATGTTGAAGCTGAGAATTTTGAATCTTCAGATTCTC
AAGGGTTTGCCCCACCTGAGGAAGTAGTACCCTCAGCCCCACCCCTTGAAATAATGCCTTCCCCACATGA
GGAAATTAATTTTGCAGAGTCTGATAAACCAGCAATGATTTTCACTACTGATGTTTCTCAAGGCCCACCA
ATAGTTTCTTCTAGACCTGTAACCAGACTCAAAGCAAAACAGGCTCCTAGAGGGGAGGTAGAAAGTGTAG
TCCATGAGGAAATTCGCTACACTACTAAGGAGCTTAATGAGTTTGCTAATTCATTCAAGCAGAAACCTGG
TGAATATGTGTGGGAATGGATTTTAAGGGTGTGGGATAAGGGTGGAAGGAACATAAGACTAGAGCAGGCT
GAGTTTATTGACATGGGTCCTCTGAGTAGAGATTCTAGGTTTAATACGGAAGCTCGCATAATTAAAAAAG
GTGTCAAAAGTTTGTTTGAATGGTTGGCTGAGGTGTTTATCAAAAGATGGCCTACTGGAAATGACTTGGA
GATGCCTGATATTCCGTGGCTTAGTGTTGATGAAGGGATTTTAAGACTTAGGGAAATTGCAATGCTAGAG
TGGATATATTGTGTAAAGCATAATTGTCCACAATGGGAAGGTCCAGAAGATATGCCTTTCACTAGCTCTA
TAAGACGCAAATTGGTGAGAGGGGCACCAGCACATTTGAAGGGTTTTGTTCTTTCCCTTTTCCTTGTACC
AGATCTTAGCATTGGAGATGCTTCTGCTCAATTAGATGAATTAAATTCACTGGGTTTAGTTGGATTCCGA
GGTAACAAGGGCCAGGTGGCAGCATTGAATCGCCGGAGACAAGGTGATTCTAGTTATTATAATGGACAGC
GTAGACAAAAGAATGTTTATAATAACATACCCAGCAATGGTCAGCACAGGAGAGGTGAAATTTATAATGG
CATGACTCGGTTGGACCTTTGGTACTGGCTAACCAATCATGGTGTTTCCAGGAATGAAATACATAGGAAG
CCTACTGCATATTTGTTTGATCTGTATAAGCAGAAAAATTCTCAAACAAATGAAAGAAAGGCTACATTAG
ATCATGGTAAACAGCAATCTCGGCCAGTGAATCAATTTCCAGACTTGAGACAGTTTGCAGATCCAGAACC
CCTTGAATGAAGGGGTGGCCAGGTTCCGCTGAGGAAGGATCTTGATAAGACACCCAAAGGTTTTGCTGTT
ACCCTTTCTCCAGTTCTTCCCCAGAGGGACCTAAGGCCTTTTACAAGGGTAACTGTACACTGGGGAAAAG
GAAATAATCAGACTTTTCAGGGTCTGCTGGATACTGGTTCTGAGTTGACACTGATTCCAGGGGATCCCAA
GAAACATTGTGGCCCTCCAGTTAAAGTAGAGGCTTATGGAGGGCAGGTGATTAATGGAGTTTTGACTGAT
GTCCGACTCACAATAGGTCCAGTAGGTCCCCAGACACATCCTGTGGTGATTTCCCCAGTTCCAGAATGTA
TAATTGGGATAGATATACTCAGAAATTGGCAGAATTCTCATATTGGTTCCCTGAACTGTAGAGTGAGGGC
TATTATGGTTGGAAAGGCCAAATGGAAACCTTTAGAGTTGCCTCTGCCAAAGAAAATAGTGAATCAAAAA
CAGTATCGTATTCCTGGAGGCATTGCAGAAATTACTGCCACTATCAAGGACTTGAAAGATGCAGGGGTGG
TGGTTCCCACCACATCTCCGTTTAACTCTCCTATCTGGCCAGTGCAGAAAACAGATGGATCATGGAGAAT
GACAGTTGATTATCGAAAACTAAATCAGGTAGTAACTCCAATTGCAGCTGCTGTACCAGATGTAGTTTCA
TTACTTGAGCAAATTAACACATCTCCTGGCACCTGGTATGCGGCTATTGATCTGGCAAATGCCTTCTTCT
CAGTACCTGTCCATAAGGACCACCAGAAGCAATTTGCTTTCAGTTGGCAAGGCCAACAGTATACTTTCAC
AGTTTTGCCTCAAGGATATATTAACTCTCCTGCCCTGTGTCATAATTTAGTTAGAAGGGATCTTGATCGT
TTGGATCTTCCACAAAATATCACATTGGTGCACTATATTGATGACATTATGCTGATTGGACCAAGTGAGC
AGGAAGTAGCAACCACTTTGGACTCATTGGTAACACATATGCGTATCAGAGGATGGGAAATAAATCCAAC
CAAAATTCAAGGACCATCTACCTCAGTGAAATTCTTAGGAGTCCAGTGGTGTGGAGCATGCAGAGATATT
CCTTCTAAGGTGAAAGATAAGTTATTGCACCTGGCCCCTCCTACAACCAAGAAAGAAGCACAACGTTTAG
TGGGTCTATTTGGATTCTGGAGACAACACATCCCTCACTTGGGTGTGTTACTTAGGCCTATTTACCAAGT
GACTCGGAAAGCTGCTAGCTTTGTGTGGGGCCTGGAACAGGAGAAGGCCCTTCAACAGGTCCAGGCTGCT
GTGCAGGCTGCACTACCACTTGGACCATATGACCCAGCAGACCCGATGGTACTTGAGGTGTCTGTGGCTG
ATAGAGATGCTGTTTGGAGCCTCTGGCAGGCCCCTGTAGGTGAATCACAGAAAAGACCTTTGGGATTTTG
GAGCAAAGCTCTACCATCATCTGCAGACAACTATTCTCCCTTTGAAAAACAGCTCTTGGCCTGCTATTGG
GCCTTAGTGGAAACTGAACGTTTGACAATAGGACACCAAGTTACTATGCGACCTGAACTACCCATCATGA
GCTGGGTACTATCAGACCCTGCAAGTCATAAAGTGGGACGCGCACAGCAGCAGTCTGTTATCAAATGGAA
GTGGTATATACGTGATCGGGCCAGAGCAGGTCCTGAAGGCACAAGCAAGTTACATGAAGAAGTTGCTCAA
ATGCCTATGGTTTCTACTCCTGTTACACTGCCATCTGCTGCCAAACATGTGCCTATAGCCTCATGGGGTG
TTCCCTATGATCGACTGACCGAAGAGGAAAAGACTAGAGCCTGGTTTACTGATGGCTCTGCACGTTATGC
AGGCACCACCCAGAAGTGGACAGACAGCTGCAGCATTACAACCCCTTTCTGGGACAACCCTGAAAGACAC
AGGTGAAGGGAAATCTTCACAGTGGGCAGAACTTCGGGCAGTACACATGGTATTACAGTTTGTTTGCAAG
AAGAAATGGCCAGATGTACGATTATTCACTGACTCATGGGCTGTAGCCAATGGATTGGCTGGATGGTCAG
GGACTTGGAAAGATCACAATTGGAAAATTGGTGAGAAAGACATCTGGGGAAGAAGTATGTGGATAGATCT
CTCCAAATGGGCAAAGGATGTGAAGATATTTGTGTCCCATGTAAATGCTCACCAAAAGGTGACTTCAGCT
GAGGAGGAGTTCAATAATCAAGTGGATAAGATGACCCGTTCTGTGGACAGTCAGCCTCTCTCCCCAGCCA
TCCCTGTCATTGCTCAATGGGCACATGAACAAAGTGGCCATGGTGGTCGAGATGGAGGTTATGCTTGGGC
TCAGCAACACGGGCTTCCACTCACCAAGGCTGACCTGGCTACAGCTGCTGCTGATTGCCAGATCTGCCAA
CAGCAGAAACCAACACTGAGTCCCAGATATGGCACCATTCCTCGAGGTGACCAGCCAGCAACCTGGTGGC
AGGTTGACTACATTGGACCACTTCCTTCGTGGAAAGGACAGCGTTTTGTTCTTACTGGAGTAGATACTTA
TTCTGGTTATGGATTTGCCTTTCCTGTACGTAATGCCTCTGCTAAAACCACCATTAACGGACTGACAGAA
TGCCTTATCTATCGTCATGGTATTCCACACAGTATTGCTTCTGACCAAGGAACTCATTTCACAGCCAGAG
AAGTACGACAGTGGGCCCACGATCATGGAATTCACTGGTCTTACCACATTCCCCATCATCCTGAAGCAGC
TGGTCTGATAGAAAGATGGAATGGCCTTCTGAAGACGCAGTTACAGCGCCAATTAGGTGGTAACAGCTTG
GAAGGCTGGGGTAGAGTTCTTCAGAAGGCAGTATATGCTTTGAATCAGCGCTCGATATATAGTACAGTTT
CACCCATAGCCAGGATTCATGGGTCCAGGAATCAAGGGGTGGAAAAAGGAATAGTTCCACTTACTATCAC
TCCTAGTGACCCTCTAGGAAAATTTTTGCTTCCTGTCCCCATAACTCTAGGTTCTGCTGGCTTAGAAGTT
TTGGCTCCAGAGAGGGGAGTGCTCCTACCAGGAGCTACAACAAACATTCCATTGAACTGGAAGCTCAGAC
TTCCCCCTGGTCATTTTGGGCTTCTAATGCCCTTAAACCAACAGGCTAAAAAAGGAGTAACAGTGTTAGG
AGGGGTGATAGATCCAGATTACCATGGGGAAATTGGATTACCTCTTCACAATGGTGGTAAGCAAGATTAT
GTCTGGAGTGTAGGAGATCCCTTAGGGCGTCTCTTAGTACTACCATGTCCTGTGATTAAAGTCAATGGGA
AACTACAACAGCCTAATCCAAGCAGGATGACAAAGGACACAGACCCATCAGGAATGAAGGTATGGGTCAA
TCCTCCAGGAAAAGAGCCAAGACCTGCTGAGGTGCTGACTGAAGGAGAAGGAAATATAGAATGGGTAGTA
GAGGAAGGTAGTTATAAATACCAATTAAGGCCACGTAACCAGTTGCAGAAACGAGGATTATAAAGTAATA
TGAATGCCCATTGTAAATTTACTAATGCGTTTGCGATTGTACGAGGGATAGTTATATCATGTTAGGCGTA
TTTACAAACTTGTTATTGTTTTATGTGAACATGAGATATTATTTGTGTCAAGTTGACAAGGGGTGGATTG
TAGTGGCTATTCCTGGTTGTCAACTTGACAATATTTGGAATGAACTACAATCCGGAATTGGAAGGCTCAC
CAGTGACCCTTATCTGGAGGCTTGGAGATCCTTATCTGGATCTTGGTTTGAAGATCTTGAGCCATAGTGG
CTATGGATTCCAGAAGATTGAATCTCCGAGTTTAAGGAACACACCTTTAATCTGGGCTACGCCTTTCATC
TGGGATTAAAGGTGTGGCGGAACACACCTTTAATCTGGACTACACCTTCTGCTGGAGACAATATAAGGAC
ATTGAAAGAAGGGAGTCTAGCTCTTGCTCTTGCTCCTTCGCCTGCTTGCTGCGTGAGACTGAGTAACTAC
TAGATCCTTGGACTTCCATTCACAGCTGCGACTGAACAATTGTTGGGAATTGGGCTGCCGACTCTAAGTC
ATCAATAAATTCCTTTACTATCTAGAGACTATCCATAGTTCTGTGACTCTAGAGAACCCTGACTAATACA
TCCCTACATACAGGAAACTTGATGTATAAGTAAAGAAAATAGTAAATCTAAAAATTTCCTTATCAAATAC
ATGCAAGAAATCCAAAATACCATGAAAAGACCTGATCTAAAAATAGTAGGAACTGAAGGTGAAGTGTCCC
AGCTCTGAAAACTAGAAAATGTTTTCAATATTTTTCCTAACAGTGAAGACATGGATCTAATGAGGCCACC
TCTTATAGCCATGCAAGACTGACAGTGGAGGGATAAGGACACCAATCCACCCACAAAACTTTTGACCCTA
AATCAGTTTTATCTAAAAGCAATGCAGGGGCACAAATGGAGCAGAGACTGGAGGAATGGTCAAACAATAA
CCTGTCATATCTGAGACCCACCTAATTGGCATACACCAGTCACTGACATTATTAATAATGTTCCATTATG
CTTACAGGCATTTCTCTAGCATTACTATTCTTGGAGAGACTTCACACAGCATCTTATTGAAACAGATGCA
GACACCCAGAACCAAACATTGGATGGAGATTGGGATCCTTAAAGAAGAGTTGGGAGGATAATTGAGAGAC
CTCAAAGGAATAGCAACCCCATAGAAAGACACGAACAGGGTCAGTAAACCTGGACCCATGGGGTATCTCA
GAGACTGATCACCAACCAAAAATCACAGAGGGCCTGGATTGATTCCCCTGGCACACATGTAGCAGAGGTC
TGCCTTGTCCTTCAGTAGGTGAAGATGAGCCTAAGGCTTCAGAGACTTGATATATCAGCATACAGCAACA
CCCAAGTGTGTCCCACTATCTCAGAGGTGAAGGGGTGGAGATGGGCAGAAGGGTCCTGATAGGGGACAAC
TGCAAAGAGGCAATATTTGGGACAGAAAGAAAGGAAGAGAGAGGAAGATAAAGAGAGAGATGAAGGAAGG
AAACAAACAAACCAAGATGAAGAGAGAGAAAAGGGGGAAAATAGCCTTGAATGGTTCAGGGATTCAAAGG
GACATACCTAAGCATGGTAAAACAATGCACATAATGGCAGCAAGTAGCTAACACTGAATTAAATAGAAAG
ACACATAAAGCAGTTCTACTAAAGTAAGAGACAGAGTTGCCCAATCTCACCCTATTTATTCAATAATAAT
GTACTTGAAGTTCTAGCTAGGGCTGTAAGACAACTCAAGGAGATCGGAGGATACAAATTAGAAAGAAAAA
TTCAAAGAATTGTAATTGGTAGATGATAGGATAATATACATAAATGACCTCAAAATTCTTCCAGAGAACT
CTGAAAGCTGATAAATACCTTCAGCAAAGTGGCTAGAAACAAAATTACTTCATAGAATTCAGGAGCTATC
CTTTATAGAAAAGTTAAAGTGGCTGAGAAAGAAATTAGGAAAATCACATTCTTTGCAATAACCAAAAATA
ATATGAATTAGCTTGGTGTGACTCTAATCTTGCAAGTGAAAGATGTGTGTGACAAGAGCTTCAGGACCCT
GAAGAAAGAAATCAAAGAACTCAAAGATGCAAAGTACTCTAATGCTCATGGATAGGCAGAATTAACATAA
TGAAAATGCCAAATTTAACAATTCAATCTACAGATTCAGTGGAATTCCCATTAAATATCCAACCCAATTT
ATTACATTCTTGAAAAAGCAAATCTCAACTTCATATAGAAAAACAAGAAATTGAGGGTAGCTAACAAAAT
CCTGAACAATGAAAACACTTCAGGAGAATTCACCATCCCCTACCTCAAGCTGTATTTTAGAGCAATAGTT
ATTAAAACTGCATGGTATTTGTATAGAAACAGATATGATGATCAATGTAATTGAATTGAATACACTGACA
TAAAACCACACTGTTATGGACACTTGATTTTTGACAAAGAACCCAATAATCATAATAAAAATCATAATAA
GAATGCATCCCCCAACAAATGGTCCTGATCTAAATGCAAATAGATTCATATCTATCATCCTGCACGAAAT
TCAAATCAAAGTGAATTGCAGACTTCGACCTAATACTGGATTAACTAAATCTAAATGAACAAAAAGTAGA
GAATAGTTTTGAACTCATTGATGCAGGATTCAATTTCCTGAACAGAACCACAATGGCTCAGGCTCTAGGA
TCATAAATTGGTAGATAGGATCTCATGAAACTGAAAAACTTCTGTAAGGCAAAGGCAATAAAACAAAATG
GCAACCTACAGATTGGAAAAAAAATAATCTTCAGTATCCCTAAATCCCATTGAAGGCCAATATGCAAAGT
ATATAAAGAACTCAAGATGTTATCCTCCACAAAACCAAATAAATCAATTAAAAATGAACTACAGAGCTAA
CAAGAGAATTCTCAACAGAGGAATCACCAATGACTGAGAATCACTTAAAGAAACATTCAATATTTGTAGT
CACCAGAGAATTGCAAATCAAAACTACCCTGAGATTCTACTTTATACCAATCATAATGGTCAAGATAAAA
ATTCAAATGACAGCGCATGTTGGTGAGGATGTGTATACTTTTGCATTGCTGGTGAGGAAAACAATCTGGC
AGCTCCTCAGAAAATTGTAAATAATTCTACCTGAAGATCCAGCCATACCACTCCTGTGCATATACATAAA
ATGTGCTCCACCATACCACTAGGAGATATGTGCCACTATGCCCATAGCAGCCTTATTTGTTAATAGCCAA
AAGATGGATACAACCCTGATGTGCCTCAAACAAAGATTGTATATAGAAAATATGGGTTCCCTTACACAAT
GGTATTCTACTCAGCAATTAAAACGTGAGAACATCATAAAGTTTTCAGGCAAACTGATCAAATGAAAAAG
TATAATCTTGAGTGAGGTAACAGAACCAAAATGACCTGCATGGGATGTACTCACTGGTAAGTGAATATTA
GACTAAAAGTATAGAATATCAATGATGAAAGCCACAGACCATAAAGAGTTTAATAAGATGGAAAGCTCAA
GTACGGATTCTTCAATCCAATATAGAAAGAGGGACAAAATAATCAGGGGAGGCAGAGGGAGAGAGAGACA
TTTGGTGGAAATGGGAGAAGAAGGGAGAAAGGCAGAAAGGATCAGGCGTTGGGGAACACTGGAAAGCAGC
CCAGGGGTCCTGGTAAATGGATTAAATATTCAGCTGAATGGAGGTGGGAGGCAGGGGGAAGCTCTGCAAA
GTCCAAGAGACCTGGGATGTGAGAGGCTCCCCAGATGATAATCTTAGCCTTCATGCCTAACAGTTGTAGA
TAAACCCTGAAGAGATCACTTCCAATAGATGCAGAGGGCCCTAAGTGGTTGGATGGAGTCCCCCAACTTA
CCTCAAAATTGTCAATACTCATAGAAATTAGGACAATGAAAATCAAAACACCCTGAGATTGTATCTTATG
TCTGTCACAATGGTTAAGACCAAAACCTCAAGTGATGGCTTGTGCTGGCAAAGATTCAGAATAGTAAAAG
TCTCCCCATGGCTTGTGGGAATGCAAACTCTTACAACCTCTTTGGAAGTATATTTGATTGTTACCTGGTA
TAAAATGGCAAAAAAAAAAAATGGTTGAAGGGGGACTAAAAAAGGAAGAAAGGGGAGAACTATGGGATGC
GAAACAAGAAAGTTTGTTGCAAAAGAAATATGTTTCCACTGCAAACCCTGAGTCTCAGACAGAAGGGGAC
CTGGAATTCTTCAGATACAAAGAATCTCTAAACCCTGAGGACATTCTATCACAAATAAGTAAAATTCAGA
AAATTCTGAGTGCTCCCATCACGGAGATGAATCTGCTATGAACAGCTCATAGGTGTGACCCTCTACAAAA
GCCATATTATTGAAAAGCCACATTGTGCCCAGACTTTGGAAAGACTGAGCTCATATCCTGAAATACAGTT
ATGTGTGGTTCTATTTAATTACACATTTACACTAAGAAAACATGGCAGTATGGGAATGAAGCTTGTTCTG
TACACATTAACAGAGGGAAACTAAACAAAGTATGGTGAATCCCTAACCAAAAGTAAAAAAAAAAAAAGAA
AGAAAAGAAAATAAAAGTGAAACTACAATATGTTTCAAATGCTGTAACTGAAATCTGGTTTTTTGATGCT
TATATCTGGTATCATCAGTGACTTCAGATTTAGTCCAACTCCAGAGCATGGTATAGCAGAAAGACATGCA
AATAAGCCTTCTCTCTGCCCATGAAAAACACCTCGGCCCTGACCCTGCAGCTCTGACAGAGGAGGCCAGT
CCTGGATTCGATTCCCAGTTCCTCACATTCAGTGATCAGCACTGAACACGGACCCCTCACCATGAACTTG
GGGCTCAGCTTGATTTTCCTTGTCCTTGTTTTAAAAGGTAATTTATTGAGAAGAGATGACATCTGTTGTA
TGCTCATGAGACAGAAAAATTGTTTGTTTTGTTAGTGACAGTTTTCCAACCAGTATTCTCTGTTTGCAGG
TGTCCAGTGTGAAGTGAAGCTGGTGGAGTCTGGGGGAGGCTTAGTGCAGCCTGGAGGGTCCCTGAAACTC
TCCTGTGCAACCTCTGGATTCACTTTCAGTGACTATTACATGTATTGGGTTCGCCAGACTCCAGAGAAGA
GGCTGGAGTGGGTCGCATACATTAGTAATGGTGGTGGTAGCACCTATTATCCAGACACTGTAAAGGGCCG
ATTCACCATCTCCAGAGACAATGCCAAGAACACCCTGTACCTGCAAATGAGCCGTCTGAAGTCTGAGGAC
ACAGCCATGTATTACTGTGCAAGACACACAATGAGGAAATGTTACTGTGAGCTCAAACTAAAACCTCCTG
CAGAGCACCCAGGACCAGCAGGGGGCGCAGAGAGCACATGGAGTTCTGATTCACAGAAGAGTTACAGCCT
GTACAATTAGACCCAATCTTCAACAAACCGTCAAAATATTCGATCCAAAATTGTTCCTGTCTAAAAGTAA
TTCAAGGACAAAATGGACCAGAGACTGAAGAAATGGCTGACCTGTGACCCTCCCAACTTTGGATCTATCT
CATAGGCAGGTACCAAACCTTGACATTTGTCACTGACACTGTATTGTGCTTGCAGACAGGAGCATAGCAT
GGCTGACCTCTAAGAGGCTCTGCAAGCACCTGAATGAGACAGATGTAAATAGAGTGTACAACTGGATTAA
GTTTGAAGACTGCAATGGAAGAGTTAGAGGAAGGAATGAAAAATCTGAAGGGGATCACAACCCCAGAAGA
AGACCAACAGTGCCAATTAAACTGGATGCCTGGGAACTCCCAGAGACTAAGCCACAAACCAAGAATCATG
CAGGCTGGTCTGAGAATCCTGGCACCCAAGTCTCAAGGAACTGCCTTTTCTTGGCTCAGATGGGAGAAGA
TGAGCCTAAAGCTTTAGACTTGACGCCCCAGGGATGGGCAATACAAAGTTGAGTTTCTTGGGAAAGTGAA
GAGGATGGTGGGAGGAGGAATAAATCTGGGAAGTGGGACTGGTTGGGGACAACATATTGGTCCTAAATTT
ATGAAATAATTATCTAATTGATAAAAAGAGTCCTTGGGGTAATGGAGGGCTAGACTCCTCTGTGCCTAGT
TTGTAACTCTACAGGGATCCTCTTTAAAAGAATAGGGTGCACATAGAGTATATGTGTGTGACACTAATAC
AGGGGTAAGTGTTTCTGTAAGAACTTTATGAATAAACTTTATTAAAACATCAAAAAGTATAGGTTGTAGC
AACCCTTGACCTGTACGAATGTTTATAAAACTTTCTATTTTCCTTAATTATATCTGTTTTGCATTTGTTT
ATTTATTTATTTACTTACTTACTTATTTATGATTTCACTGTGTCTTTCTGTAGTCCTGTATGTGTTTATT
TGTCTCTTTATTGCTCTGTTTCTGTTTCTTTCTCTCTGTCTAGTATTGTATCTCTCTGTGTCTCTATGTC
GCCGATTGTGTCTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGCATATTGAAA
TGAGATCTCTTCTTCTATCAGTGTACATAATGACTTTGAAATAAAGTCCATCACTGGGCTTGGATATCAC
TATCTTGTTAGAATGGTTGCCCAAAGTGTTCCAACCATCACACTGTTTCTACATATAATAGATGGGTTTA
TTGATTTACTACCAAAACTACCCCATCTTAAGTTATGGATATTAAACACAGATCCTCATGGTGTGTTAAG
AACTTGAAGCACAGAGCTATATGTACAGCCCACTAAAAATGACTTAAATATAAGATAAACTCTGGCTTGA
ACAAAACACAGAGCATCACACGAGGCAGAAGTGAATCTCTATGAGGACCAGGATCACATCCCAAAGAACC
AAAGAACACTTGAGACTGTGGAACCCTAGAGTCAGGGCTGACACTCAGTAAGATTTACTTCATAGAGCAT
GATGTGGGATTCAAGAAGAAATCAGGCAGGGCCTGTGACCCATGAACCAGATGACTTCCAGTCTCTCCAT
CATCCTCACACAAACCATAGGCAACAGCTCAGCACTGACTCACCTGTGATGCTGGTGACAAAATGTGTGC
AAAGCCTCAGAACTGAGACGTGAGGCCAATGTTGCTACCGACACTCAGTGAGCTTCAATTTGCTGCAAAC
TGCTCCAAAACAACTCAAAGGGTAACTTCTTCTTTTTCTATTTGTACTTGTGATGCCTTGGTAGATATAG
TTCCCCAATAATTTAATAGGACAAATCATTTTTCCTTTTAACACATTAATCCCCTTTCCTTCCCCCAAGA
TTCTAAAATTTTTCTTTAACACTGATGTCTGAGAGATATGGCATAATAGATGTTTGTCTTATTCTGAGTT
GTTCCTTATCAAAAGAAATCAGCTGTGGCACTTTGAACCTAAATATAACAATATTTCACAAAGGCATTCT
GGGAATATATAGATGGTATTGCTACAAAAGAATAGGAGGATCTATGGCCATACCACCCTTAATGAACCAG
ATACTATCTAAAAGATTAGGACAGCTGGGCATGTTGGCACAGGCCTTTAATCCCAGCACTAAGGAGGCAG
AGGCAGGCAGATTTCTGATTCGAGGCCAGACTGGACTACAAAGTGAGTTCCAAGACAACCAGGGCTATAC
AGAGAAACCCTGTCTCGAAAAAACAAAACAAAACAAAACAAGAGTAGGAGGGTATTAGATGCGAGGTGAT
GATGGCCATTCAATCAAACCAGAATGTGACCTGAGAAAAGGTCTAAGTTTGTCAGCTTGTCCTGGAGATG
TGTTGCCTCTGATAAATGCAGAGCTGGAACTGCAATACATTGGATTCCTGTGAGTTACGGAGAAATAAAA
GGAATAGCCTCTGTTTCCTTTGGTTCATCAAGTTGGCAGTGAGCTAGAAGATGATACTGGGAACCATCAC
ATGTACTTCTACAGAGTTATTCTACATTCTACAAGTCAGTCTTGCTTGCTTTCGTTTCTGTGAGGAGCTA
AATACAGAATGTTTTCAATGTCTCATAATGTTCTGTGCACACCCATGGGTGCCTAGCCAAGGATAGAAAC
TTGTATCTGATAAGGAAAGAGGATGCTGCAGTGGCCACACTCTGAGATCACCTCCTGCCAAATTATACTA
GAGTGTTTTACATAGTCTTGAAAACACTAGGTCAAATATGTTGTCATCCAGATATGAAAACTCTGTGCAA
CAATTCAGAAATACAGTGGTCAGGACAACTGGTATGTAACACTTTAGTTCCAGCCCACATTAATCTCACA
TCTTTTCTGTGGTTTCAATAAAGAGCACACATGTTTGGAACACCATCTCCACTTTGTATGCTTATCCTTA
TGCATCAAACTTTTCCTGTAATTTCCTACCCCGCACAGGTTCCTGTCTTGATCTCTTTTTCTAATGAACA
GCAATACAGAAGTGTTGGCCAAATGAACACTTTCACCCCAAGTTGCTTTGCCATTGTGTTTCATCACAGC
ATTAGTAACTCCCAGTAAGGCTACTGGTTACCCTGCATCTTCTATGTAGATCTTTTGATGAAGTTCCAGG
CTTGGGGTCTTTATCTGACTGTTATCCTGGTTAATTCATGTCCACCAATAGAATATTGTTCTCATTTTCA
ATATCATATGAAGTTACCTGTTTATAGGATCTTCCTAAAGCAGAAAACTATATAAAATGTCTGCTTACTT
TAAGAAAAGTATGTGTCCTTGGAGGTAAATGGAGGAAAGGGTGGAGGATGTTGAGTAATGGAAGACTGGC
TAATACAGGAAGGTCACAGAATTGAATGATTTTAAAAATTTTAACTCCTCATCAATGAGGTACTTCAAGT
TGAGGCAGGAGTCCTTGATGCACAGGTGACCCAAAGGCCCCAGCCTCTGTTTCTTGGTCTGCTCCAGTCT
CCCGGCTCAGCACAGTTCACTGAGTGACAATCTTTAAGATAGTCTTTCAGGATACAGTTATCACAGTTTT
CAAACTCTCTTCATTGAAACAATATAATGAGTCAGAGTCCAGTGTGCCATTAATAGGCCCTATGCAATAG
GATTTTACTTCATCTGTGGAAAGAAACCACCATGGCCTCATCAAGGCCTCATTTGATGATTAGTGTAGAA
AGCGTCCCCAGAGCTTTGCTGGGATGAAAACCCTGGCAAGATGTTTAGGGCACTCTCCATCTGACAGGAC
AATTCTATCAAGGGTTGCTTCCATATAGAGAGAAATCTCCACATCTGCAGTATTTCCCGATTTATCAACA
GTTCTTTACCCCGTGTCATATGCATAAAATCTTTTCTCTGTAGAATTTTCAGTACTAAAAACTAACAAGG
ATGTTGGTCCTGATATCAGACATAATAAATAAATAAACATCAAATTATACCCTAAACATGTGGAGATGGT
TTTCTGTTATGAATTTATTGGCTCTCTAAAAAGTGTTAATTAAGCCAGGCGTGATGGCACATGCCTTTAA
TCCCAGCACTTGTGAGGCAGAGGCAGGCGAATTTCTGAGTTTGAGGCCAAACTGATCTACAGAGTGATTT
CCAGGACAGCCAGGGTTACACAAAAAAACCCTGTCTCCAAAAAGTAACAAAAAGAAAGTGTTAATTAACA
TCTACACTTTAAGATAATTTGGTGGCAAAGGTTAATAGTCTGAAATACTAGATATGAAAGTATGACACCT
AAATTGCATGCAGAGAATAAAGCTGAATTCCAATGAGATTCATGCCTTGTGAATCCATTCCAATCTGATC
TCAATCTTCAAACATGTTTGTGCATAATTTACATCTTAATCAGTTGCTGGAAATTAAAAGGACATAAGCT
AAACTTAAAACTCTTTCTATTTAATGTGTCAGAATAGAATATGTGCACTGAAGTTTTCATGTGCATAGGA
TATCATTGTTACAATGATGGTTCAAATACGCACCAGTAGCACAGGTGATGCTTGACATGAAAAAAACTGT
GACCAGAGCCCAGACGTTGATGTTGGCTGAAAGAGCCTAGTGTCTCACATTTGATCAAGCAAAGTGTTAT
TCAGAGAAGCAGTGCCAGATTTGGGATCCTTCTTAATGGATCAAGAACATGAGGAAGATTTGGAAGCCAG
ATTGATGAGATTACTAGAATCCTGGATCTGAACACACAACACACAATAGTAAGTTGAACTTTTCCACGCA
ATGCTAAAGATAAAGCATCCAGTATCATGTTATACAGGACCAAGGTGAGGATCAGTCTGTGGGTGAAGTA
CAATGAACTGGAAATTGTTTGGATTTTAAATGTTGGTTTTTAAATTATATATAAAATATATATATATATA
TATATATATACATATATATATATATATATATATATATATATATATCGGAACCAACATTTAAAATTTGGAT
TTTAAATGTTGGTTTTTAAATTATATATAAATTATATATATATATATATATATATATATATATATATATA
TATATATATATATATCGGAACCAACATTTAAATTCCTAATTCTTAAGGACTTCACTTTTGACCAAAAATA
TTTGAAATGGGTTATTTAGGTCTGTGGTGGTTTGAATATGCATAGCCTATAGGAAGTGGAAGTATGGCCA
TTTTGTAGTATGTGTAGCCTTGATGGAGGAAGTACATCACTATATGGTGTTGTGAGAGTTGAGATCCTAT
GTTCAAGTTCTGCCAAAAACAGAAGAGAGTCCCCTCCTGGATATTTGAAACAGATGCAGAGGCCCAAAGC
CAAACATTGGGTATATCTCAGGGTTTCTTATGGAAAAGTTGGCTGAAGGATTGAAGGCCTTAAAGGGGAT
AGGAACTTCACAGGAAAACCAACAGATTCATCTAACCTAGACTCTAGGGGTCTTTCAAAGGTTGAGTCAC
CATACAAAAAGGACACAATGGCTAGACCTAGGTCCCTGATACATATGTAGCAAATGTGCAGCTTAGACTC
CATGTGGATTCCCCAACGACTAGAGCAGGTATTGTCCCTGAAGTTATTGCTTGTCTGTAGAATACATTTC
CCAACATAATTGTCTTGTCTGGCCTCAGTAGGAGAGTATGTTCCAATCCTGGAGAGACTTGATATGCCAG
GATTGAGAGATTAAGGGTCTCCCACCATCTCAAAGTTCATGGGTATGACAGGGGAGGGGCAGTGCAGGTT
TTCCCCATCTCTGATTTCCAGCATTTGCACCCCCAATATTGAGGGGGAGGTCTAGCAATTGGAGGGGGGA
CAAACAGTATGAGAAAGCTAACTTTAGTGGTGTTGTCACAGTGTGGGGTATTGTGGGTGGGAGTGGAGAT
GAATCCTCCTCCTCCTCAGGAGGCTGTAGCAGGTGCTTGCCCTCTTTTGGGACAGTAGAGCTGTGGTCAG
TACTGGCCTGTTCCTGACAAATTTATTCTGTGCTGTGACCATTTATTCAAGGGAGGAAGATTTAAGACTT
GCTATGATAACCTGACGAATCTGGACAAACAAGAGACAGGAGGTAACACCTGAGTTACTCTTTTGCTTGC
TAGTACAGAAGAGCACTATAATCCACAAATTGGGGTCCCACATATTCTTGAGTGGGATCCAAGGTGCCAT
AATTTCAGCCACATTCCAAAGTCCCTCAAGGTCTGACAGTTTAGTCTTTAGGCTTCTCCTAACCAAATCA
TATTTGGAGTTCTTTAACATAAAAGGGTAGTTTCCCCATATCACTGCCAGCAAAACAGGTTGAATTTGGA
CAGACAAGCAAAACTATCACAAACAAGACAGACTATGTGTGCGATCCAAATGTCTTGTGATTAGAGGGGA
AGTTCTGCAGCTTATTAGGCTGTGTCCTTATGCCCACAAATGTCTTCAGATAGGAAAGGGCCATTGGGTG
CCAATCCTTAGTTCTTCTGTTTCCTGGGCTGTCAGTTTTTATGTGGAGAAGACATGAACCAAATGCAAAG
TCATGTATCCTACAAGAACTGACATACACAGACAAACAAGGAATAACAATGAGCTCTCTCATTCACCTAA
GGGGGTCTTATAACATAGAACAGAAAGGGGCCAACTTAGCAAGAATTGACCAAAAGGTGGTCACCTAGAC
CACACCACAATGCAGGAAGGATATCAATGAAGGAAGAACAAAGGAAAGGCCAAATCAAGTTTGTTGGCAC
ATGACATAAAACAGTGCTCAAAAAACAATAGATGCGAGCAAAATGAAGCATTGGAATCATATCGGGGTAC
ATAGGGGTCACTCTTACCCCTGTTCAAGTGCCAGATGTTGTTTTAGCCTTAGGCTCCCTGAGTTTGTCTT
TAGGGGAAATGGGTAAGAATACACAAAAATAATGTCACAGAATCTTAGAGGCAGATGAGAAGTCAAAGCA
GACTCATTTATTGCTATAGAAAAGAATGCCTTTATACCTTCTGTCCATAACACCTCTGCCCATATATGCT
CATCTCTGTGAGGCAATGGACTACCTGCAGACAGGCTGGTATCCAGTCAAGCTGAGGTCTGAACCACAAT
GGTCGTAACTCACCTAAAGGACGTGGTAGGCATGACGCGATAGGCGTTCCCTCATGCTCCTGGAACTCCG
GCCCCTGCCTAAGGTACCACCTCCCACAGCCCCCACAAGAGAAGCATGGTCAGTAGTCATGTAAGCAATG
GCCCAAGCTTCTGACCTTCAGCCTAAACTCCTCCCCAGTTACCTAGCAACAGTGAAGACCATAAAATGGG
ATGTTAGGCCCCCACCTCACTCTCTTACTCCTTTGTTCCTTTACCTCTCACTTCTCTCAATTCTCTCTCC
TCTTCCCTTTCTTTGTCTTCTTCTTTCCTTGTCTCTCTGTCTTTCTCTTTCTCTCTAGCCTTCTCTGCCT
CTCTCTCTCTCTCTGTCTCTCTGTCTCTCTCTTCTCTCTTTCCCTTGAATTTCTTTTTTTCTTTTTTCTT
TTTTTGATGAGCACAGTATAAATAGTTTATTGCATTGTTTCTGAATGTGGCTGTATTCTCTATTGTTCTC
TTTCTTATTTTTTTTCTTTTTTTGGTTGTTTTTGTTTTTATAGTCTCGCACAGAATTCTACATCTACCAA
ATCCCCATTGTGTACTTTAGTTTTGTCTTGTATTCAAAGGTTCTTTCAGAAAGAATCTTAAAGATGAAAT
TTGCCTTCCAACTTTTCAAAAATACATATAAAGATAGATGTTACATAGTTCACGGAATAGACACATATAC
GGAGATGCAGCAATATCTCCTTTGTGCCTCTTGTATATTCATGTTATTTCCTCAACTAAAAATACATAGC
CACAAAGACATACAATGAGCACTCCACTTAGGAAAATATGGCCATATTTTTTTACATTTTTATGATTTTA
TGATTAGGTATATTCTTCATTTACATTTCCAATGCTACCCCAAAAGTCCCCCAACCCTTCCCTCACTCCC
CTTCCCCACTCCCACTTGTTGGCCCTGGAGTTCCCCTGTACTGAGGCATATAAAGTTTGCAAGACCAATG
GGCCTCTCTTTCCACTGATGGCCCACAAGGTCATCTTCTGATACATATGCAGCTAGATACATATACATAC
ACGAGCTCCAGGGGGTACTGGTTAGTTCATATTGTTGTTCCACCTATAGGGTTGCAGATCCCTTCAGCTC
CTTGGGTACTTTCTCTAGCTCCTCCATTGAGGGCCCTGTGATCCATCCAATAGCTGACTGTGAGCATCCA
CTTCTGTGTTTGCTAGGCCCCATTATAGCCTTGCAAGACAGCTATATCAGGGTCCTTTCAGCAAAATCTT
GCTAGTGTGTGCAATGGTGTCAGCATTTGGGGGCTGATTATGGCATGGATCCCCGGATATGGCAGTCTCT
AGATGGTCCATCCTTTCATCTCAGCTCCAATCTTTGTCTCTGTAACTCTTTCCATGGGTGTTTTGTTCCC
AATTCTAAGAAGGAGCAAAGTGTCCACACATTTGTATTCATTACTCTTGAGTTTCATGTGTTTCCCTTGC
ATTTCTATAATAAACCATAAGGAGTCTCTGCTCTACCAAGACCCGCTGCACACTCTGGTCAGTGTTGGGA
ACTTTTCCCCTATTCCCTCTCTCCTATAACTCCGGGGCTACAGGGTGTCTCCTTTGGGTCCCGGTTGGGA
GCTGTCTCTTCTCAACCCCCTGACTCATGGGTCAGAGGCCTAAATCTCCACCCAAGGCTGTGTGAAAAGC
ACCGGGTGGTCTCCCCATATCTCCCTGTCCAGAGCACAGGAACTCTGGCCGGACATGGCATATTTTTCCT
CCCCAACTTCTTCCCCGGCCTCCTCAGGGCTGTCCCTTCATTTTGTTCCCCACACATCTCCACATGGCTG
CCACCATCCAATTGGCTGCTGAGGTCACACACTCTGTCTTTGTTCTTCTCTGAGTCTCATGCAGGTTTTG
TGTTAGTAAATGCAAAAGTGCCTGCTGTGCATGCATGAACCATTACAGGCTGCTAGGCAGAACATGCTGA
CTTGCTCCTGGATTATAAATCCATCACTGTGATAGCAGGACATATCCAGAAGACATTATTTAACAACACT
CCTCTTCGTGCTCAACCTCTCATGGTCTTTCTACCTCCTCTTTCTTAGTGTTCTCTGAGCCTACATGAAA
TGTGAACAAACCTGAAAAAAATCTCTGTGTCAAATACATAGTGTGGATTGGGAAGGAAAAACCCTGCACT
TGTGTGTGGGCAGGGAAATGCTGGTGCTTTAACAGGACAAGTGCGGTGTGGCTAGAGAAAGTGATGAGAA
GAAGGGAAGCATGAGAACTGGGTGGGTGGATCCCATGTCTACTGAAAATGCTATGTTGATGTCAAGATAT
TGTGTCTTGTTACAAAAATTCATTGAGTACCTAGGTTATTCTCAGTATATAAAAGGGGAGGAATGAAACT
AGAATTAAACACACACACACACACACACACACACACACACAAACACACACACACGAGTAGAGAAAAGAAA
TAGTCATGAGTTGCCTAGTGAATGAAGAGCGGAAAATGGTGCAGTTAATAGGGATCATAGAAAGAACTGG
GGGATGCACACGAATGGGTTTCCTGTAATTATGTCTTCATAGTAATCTCTGCTCAATAATCAGACAGTGA
CAATGTATGCCTCATTTACAAGCCCTGATGGCCTCTCAGAGGAAAGCATCTCTCTGAAATGAATAAAGTT
CAGAAATGTCCTAAGTGTCCCTGTCACAGGAAAGCAGTGTTGGTAACGTTTCCAGGAAGCTCAGTCTTTG
TCAAATATCCACAACAAGAAGAAGCCATGTCTAGACAGACAACAGACTGGGAAAGACTGAGCTCATGTAT
GGAAACACAACTATGTGTCATGCTTTTATTTTCACATTATACTGAGGGGATATGACATTATAGAAACACA
GTTTGTTCTTTGCATATGCTGAGAGAGGAACTAGATGAAGTGTGCTAATTCTTAGCAATAAAAAGATATA
CAAAGTGGTACTTTACTGTTTCAATTTTTGTAACTGGCATCATTGTCTTTGATATTTTATATCTTCCTTG
AGCACTGCCTTCTCTCAAGTGTCCAACTTCAGAGTATGCTATAGCAGGAAGACTACCAAATAAGATTAAT
TTTTTTGTACCCATGAAAAAATTATGTGCCCTGACCCCTGTTCTCTGAAAGAGGAGCCAAGTTCTGGATT
CCCAGATCCTCATATTCATTGATCAGCAGTGAACACAGATCATTCACCATGGACATGGGGCTCATCTGGA
TTCTCCTTATTGTTTTTAAAAAAGGATTTCATTGGGAAAAGCTGCCTCATATTTCTATGACCAGGAGAGG
AAGATACAGCTAGAGACACAAGCCCCACCATGTAAAATCTGTATGGTACTCTCGTTTTCATTTTACAACA
TTTTGCTTTTGCATTGCAAAGGAGCAATAAGGGTTCAGCAATCAGCACTGTAACTGTACTTTTAAAATTT
CCACCTCTACCCTCTGTGTCCTCTATCTAGTGATAGAAAGTGAAATAAGCACATAAAGCATGGGACAAGA
AGAACACACAGAGGAGCAGAGACTGGCTACAAGTGGCAGCCAGCATGGAGCTGGACAGAATTTAAAAAGA
TAAAGAGAAATGTCATGGCATGAAGCAGTGACTTTTTCTGTTCACAATGCCTGCAAAAACTTTATGGAGC
CTACTACTGCTGAGATATGCAGGTTTGGTTCCAGAGAGGATTTTCTGTTTTATTTAATTTGCTTTTAAAT
TTTTCTTCTTTGTATAGTCTGTATTTTTAAAAAGGGGTTGAATGAATACCTAAGCAGTTGGAAAATTTGT
GAGTAGAAACCGAGGGCCTGAGAAACAAATAAATCAAAATTTTGAAATGGCAAAAGAAAGGGTTCATTCT
ATTTTCTCTTTTCAGAAAAGGTTGCAAAGTTGGATGTGCAAATGGAGATGTTATGGGTGGACTTGGAACA
TCTTGGGAAGGAAGGAACCCAGGAAAAGAAAGAATGAAATGGCTCAGCCTCAATGACTGAGCAAGAGAGT
TAGAGTCACCCACAGGAGAAAAGAAGAAAATAAACAGCACTGGGGTGAATTTGGGTCAGAGAAAACCTTA
AAATGCCAGAGGACAATGTGATGAAAGAGACCATTTGAAAGAAAGGGTTAACCTCATTCCATCAGTCATG
CCAGAGTTAGAGAATGGGCAGTCTGGAGTTACAAGATCACGATTAGCCTTCCCAGTATGTATACAGCATA
TTCCTGATAATGAGATGAAGCAAAGTTTGACAAGGTAAGATTGTATCCTATAGTAATATATAGATCAAAG
GCATGTTGCAGTAAATCTCCAACAGAAATAAGCCCGGGCAATGAAAACACAACTCAATTAATATGAATAC
GTGCTGTGCACCTAGACTGGGCAGATCTACCACTCCACTACCATATGAGAGATCCCTTATAACTTGTGGT
TTCTCCAGGCCAGCTGCTTCTGCTCTACTTTCCTTCCTCCTCCTCCTATGTCATCCTCTCCCTCACTCTT
TCTCTCCCAAAACTTTCAGCTGCACCTTCCCCTCTTCATCCCCCAATCAGTGGCTCTAGCCTTTATTTAT
AAATTAAGGTAGGAAGAAGGTTTATAGGAAATTACCTGAGTGTTGACGTGTTGATAACCCCACACAAGAG
AACAAAATTAATATCAAATATGATTAGCTCCAGGGTTATATGCAACAAAGGCATGATAAAGTGGTCATGA
TTTCATATGAGATGTACTGGACTCATGTGAAAAAACGTTTAAATACATGGGCTTACTCAAAATATAGACT
CATACCCCCAAGATGGGAAGAGACTGGTAACATTTGTACTGGAGGCTGGTCTGCTGTGGCAGCCATTAAC
ACAGTGGAGGGAAGAGCCTCAAAGAGTGAAATAGAGGAAGGGGAACAAATACAGTAAACCACCAGTTACT
ACTTAAAGGGCAGTGTTCTGATGTCCTAGGGAGGGCACTCCAAAATACCAATGGCCTTATCCTTAAATTT
ATTGTTTAATGAGCCTACACTGATCTCAGTGGCCCACTACTAAGGAGAAATGACAGGCACTTGAACAGCT
GATATGAGAACAGCTGGAGGCACAGTATACAGAAGAGTTTACAAGCCTATGGAACTCCCCTGTATTTTTT
TATGATGAGGAAATCTGAAAAATGTAAAATGTTGAAAGATTTAAGAGCAGTTATTAGAGTAGATCAACCA
ATGAGTCTCTTGCAGCCTGGAATTCTTTTATCTTGTTTGTAACAACAATCAGGCTCCATAAATTATAAAC
TATAAAAATTTACTTTATAAATTATAAATATATATATATGTGTGTCTGTAGATAGATAGATAGATAAATA
GATAGATAGATAGATAGATAGATAGATAGATATATGGATTTGATGGTTGCAGCAAATTCTAATTGGTTCC
AACAACCCTACTCACATAATACATTCATACAGGTTTGATAGATGGAGCTAAGTTTTAAATAAATTATTTT
TCTCCCATGGCTTATATATACACCACCAAAATTCTCAAAAATTAAAATGTGATTGTGTTGTATTTTTCTT
TTACTGAATGACTATAAAAAGTTACAACATTCTCCATAAATTTACATGAAAAAATATTATGTAGTGCAGG
TAAAGAAAACAAATTGACCCAAGAATGGTGTATATTCATTACTAAGCAACTTTTTAGATTCACAAAGTGT
GGGTAAACAAGGTAATTTTTTCAATCAGTTTTTTTTAACTGGCAGGCAGCAATTCAGAGTTACAATGAGA
AGATTAATAATTTTATTGTGTATTTTAAAATAAATCTTACAAAAATATTAATAGAATCACAAATTTATAC
CTTTGTATAAAAACAATCAGTCATTTCTACTTTAAGAAACAGAACTCACATCTACTCATCAATCATTTTA
TTAAGTCATATTACAAAGCTGAGTGCTAACATGGGTATAAGAAAACCATAACCTTATTCACCAGCCCAGC
GTCAAAAAGAAAAAAACCAGTCATATCAGCTGCTGCTTCAAGAGTTCTTGTTCCTTGACATTAACAAAAT
CCCTAGCTTAACTATTAAATTTTTTTTCAAAACTTCTAATTGATCCCTTAGATAAATGTTTGTGCTAACC
ATCGGGACACATCCCATGAGTTCTGAAGCAGTGTGTTGTTCTTCATGCATGGCCCTTTTGTAGAGCTGTG
AGTGTAGGGGAAGAGGGGGGAGAGAGAGCCCGTGTCCAGCCAGAGATCCTGTGCTCTGGGCAGGCAGACA
CGGGAGGACAACGGAACACTTTTCACTCGGCCTTTGGTGGGCATCTGGCTGTGTGAAGTTACTGACCCCA
CATGGTGGGGGATGGACAAGGGGCAGCCCCTGGTACCAGGAGCCCCAGGGCTACACTCTCGGCCCCAGAT
ATACAAGAAGAGGGCAGAGGGAGAGAGGCTCCCACACAGGCGAGAGTCCTTAGTCTGGTCTGTGGCTGGA
GCAGGGGAATTCCTTCTGATTGGAGATTAGGCACAGCTGATTAGGGGAAAGCCTACCCCATCATCCAAGC
ACAATGGACTTTGAGGAACAGAGCCAGTCTAAGCTTTTATAGCTTTATGGTAGAAAGGCAGGGAGAAAGG
AGAGAATGTGGAGAGAGACAGAGAGAGACTGGCCATGGCCAAGAGGAGGGAAGGGGGAAGAGAGAGAAAC
AGGAAAAGCTAGAAAGTAAGATAAGAGAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGCAAAGTG
GGGCCAAGCAGCCCCTTTTCTAGTGAGCTTGTCATCTCACAGTTGCTAGGTAACTGGGGAGGAGTTTAGT
CTGAAGGTCAGAAGCTTGGGACCTTGTGTATGTGACTACTAACCACAGCTTCTCCTGTCGGGACTGTGGG
AGCAGTAACTTTGACAGGAGACAGGGATCCAGGAGACATGAGGGAACACCTTCTGTCCCACATAGGTGGA
ATCGGTTGTACCAGGGTTCAGAACTCAACTCCACTGGAGACCAGCCTATGTGTGCATAGCCCATTGCCCT
ACAGCCTTTGACATTCTACAGAGTTCTGAGACTGGTAGAGATACAAAATAGCTAGGATTACACAGAGAAA
CACTGGCTTGACAATAAAACTTCCAAAACAAATATAAATGAATAAATACACAAATTGGAGCAAGTGAACT
TGCACTAAATCCATCAAAACTTGCACAGGATGGCTGTCTCCATAATGTTCAGATCTACTTCTATTAATGT
CATTAAGAAGACAAAGATTATGCACAAAAAGAAACAAAACAAGAAACCCAAAAGGTAAAGGCTCCAATTG
TAAGTAATAGACCCACAGTCTCTGAGGCCTGACTGTGCAGGTCCTCACCTTTGGTAATCAGCACTGAATG
CAGACCACTCCTCATGGACTTTGGGCTCAGGTTTGTTTTCCTTGTCCTTATTTTAAAAGGTAATTCATAG
AAATGAGATCCTGCCAGTATTGTGTACATGAGAAATAGAAAAATTGGTTTTCTTTGCTCTATTTTGTTTT
GTTTGGTTAATGACAGTTTCCAAATCAGCATTCTTTGCTTTGAGGTGCCCAGTGTGAGGTGAAGCTGGTA
GGGTCAGGGCAGCCTGGAGGGTCCCTGAAACACTCCTGTGCAGCCTCTGTAGTCACTGTGAGTGACTACT
GAATGACCTGGGTCCTTCAGGCTCTAAAGAAGGGGCTGGAGAGGGTGGAAATAATTTTTAATGGTGGAGG
TAGCACCTATTATCCAGACACCATGAAGGGCTGATTCACCATCTACAGAGATGATGCCAGAAACACACTT
TACCTGAAAATAAACAGTCTGAGGTCTGAGTACACAGCCATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
TGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTATTCATATATATATATATAGTGTGTATGTATATTCATAT
ATATATGTATATTCATAAGGTTGAGTAAATGGACTTAAACTGATTCCATCACAACTTCCATGGGATGGAT
ATTTCCCAGTGTTAAGACCTGTCAACATCACTGTCATTCAGGAGACAATGATTATGCACAACAAAACAAG
AAACCCAAAAGCAGAGGACTCCAATTACAATAGACCCAGACCCACAGTCTCTGAAGATTGACTGTACAGT
TCAACCCAGCCCTGTACTTCTCTTCCCTAGAATTTACATTACTGAGTAACTGAGGAAAGCTCTACAATAT
CTGTTCTCTATAGTGGTCAACACCTCCAAACACAGGTTACCCATATTCATGCCTGCCTTCTGCTACACTT
CTTGCCATAATGTAGACTACTTCAGCCTATTTTGTACTCCAGTTAACGAAACTCAAGACTAGCTGCATGT
TAGTCCTTATTCTGAAATATTATGAACAGGTGACCTCCCATCATTCACCAATGCAATAATCATATTTAGG
AATATGAGGTTTTATGAGATATAAGCACAAGGGAGAGAAAGTAAGAAAACTACATAGATATATAGACTGA
CATAAATCAAGACTTGCATGAGCTAGTGCCCAAGTACCATGTCCCTAAGTGGCAAGGAGTATCTTTTGAG
CCTAGTGAGATGAGGTACAAATCGGACTCTTACATCTTTTTAGATAAACATGTGAGATCAATGGACTAAA
GGCGTGAGCTGGGCTTCCTTGCAATCCATTTTCCATACAAGATAACAATAGATCTGGCTCCACAGACACG
ATGAGAATAGTCTTAATTAGTTCTTTAAGTAGAATGACTGATCACTAAGAGCCCAATTCCATACTAAATA
CTCTTCTGGATTATGCACAGATAAAAATTGCACATAGGGCATGGGGCACTGATCTCCCTGCACTACATGA
ATGGGGGCTCATTTACTAAATGTTCCCATGTTTCTTCCTAGTGCTGCACAGAGCAAATCCTACAACTTCC
TGCTTGTCTACAATGTAAACTCCAGACAGTACCAGAAAATCATTCCTTATGTTCCTCTCCAGGTGCTTCA
ACAAGCACAGTGCAAATTTCTGTCACCCTG

Molecule Role : Vaximmutor
Additional Molecule Role : Vaximmutor
Related Vaccine(s): S. pneumoniae RASV synthesizing PsaA

IV. Vaccine Information

1. DNA vaccine expressing PspA

a. Vaccine Ontology ID:

VO_0004516

b. Type:

DNA vaccine

c. Status:

Research

d. Gene Engineering of PspA

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

e. Vector:

pSecTag2A (Ferreira et al., 2006)

f. Immunization Route

Intramuscular injection (i.m.)

g. Mouse Response

Vaccination Protocol: Groups of at least six mice were inoculated intramuscularly with 50 μl of 10 μM cardiotoxin into each tibialis anterior muscle, 5 days before immunization with 50 μg vaccine vector in PBS (100 μl). Mice received a booster after 3 weeks with the same dose of plasmid DNA. For immunization with recombinant protein, mice were injected subcutaneously with 5 μg rPspA3NS, using aluminium hydroxide as the adjuvant, and also boosted 3 weeks after priming (Ferreira et al., 2006).
Vaccine Immune Response Type: VO_0000286
Immune Response: Animals immunized with a vector expressing secreted PspA developed higher levels of antibody than mice immunized with the vector expressing the antigen in the cytosol (Ferreira et al., 2006).
Challenge Protocol: Immunized mice were challenged by intraperitoneal injection of 200 c.f.u. S. pneumoniae strain St 679/99 (PspA clade 3, serotype 6B) in 0·5 ml saline 6 weeks after priming. Animals were then observed for 2 weeks (Ferreira et al., 2006).
Efficacy: All the immunized groups showed significant protection when compared with control groups. Animals immunized with pSec-pspA3NS showed a tendency towards increased survival when compared with animals primed with rPspA3NS (Ferreira et al., 2006).

2. Pneumo 23

a. Product Name:

Pneumococcal Polysaccharide Vaccine

b. Tradename:

Pneumo 23

c. Manufacturer:

Sanofi Pasteur Ltd

d. Vaccine Ontology ID:

VO_0010731

e. Type:

Subunit vaccine

f. Status:

Licensed

g. Location Licensed:

Canada

h. Host Species for Licensed Use:

Human

i. Preservative:

Phenol

j. Immunization Route

Intramuscular injection (i.m.), Subcutaneous injection

k. Storage

Store at 2° to 8°C (35° to 46°F).

l . Approved Age for Licensed Use

2 years of age and older.

m. Description

Products: Polysaccharide.

3. pneumococcal polysaccharide conjugate vaccine serotype 1 (PNC-1)

a. Vaccine Ontology ID:

VO_0004265

b. Type:

Conjugate vaccine

c. Status:

Research

d. Adjuvant:

VO ID: VO_0001344

e. Immunization Route

intranasal immunization

f. Mouse Response

Host Strain: NMRI
Vaccination Protocol: Mice were immunized i.n. with 0.5 or 2.0 μg of PNC-1 alone or mixed with either LT-K63 or LT-R72 (Jakobsen et al., 1999).
Immune Response: Administration of PNC-1 i.n. with either LT-K63 or LT-R72 elicited significantly higher antibody responses than the s.c. route for both 0.5- and 2.0-μg doses of PNC-1 in saline (Jakobsen et al., 1999).
Challenge Protocol: Mice were challenged with 50 μl of pneumococcal suspension i.n. and were allowed to aspirate it into the lungs for 10 min (Jakobsen et al., 1999).
Efficacy: Immunization i.n. with PNC-1 and LT-K63 or LT-R72 conferred 100% clearance of lung infection caused by serotype 1 pneumococci (Jakobsen et al., 1999).

4. Pneumovax 23

a. Product Name:

Pneumococcal Vaccine, Polyvalent

b. Tradename:

Pneumovax 23

c. Manufacturer:

Merck & Co, Inc.

d. Vaccine Ontology ID:

VO_0000088

e. CDC CVX code:

f. Type:

Subunit vaccine

g. Status:

Licensed

h. Location Licensed:

USA (License #0002)

i. Host Species for Licensed Use:

Human

j. Preparation

It consists of a mixture of highly purified capsular polysaccharides from the 23 most prevalent or invasive pneumococcal types of Streptococcus pneumoniae, including the six serotypes that most frequently cause invasive drug-resistant pneumococcal infections among children and adults in the United States (FDA: PNEUMOVAX 23).

k. Storage

Store unopened and opened vials at 2-8°C (36-46°F).

l. Contraindication

Hypersensitivity to any component of the vaccine.

m. Description

Pneumococcal polysaccharide vaccine provides at least 85% protection in those under 55 years of age for five years or longer. Immunization is suggested for those at highest risk of infection, including those 65 years or older. The vaccine is generally given with a single lifetime dose (Wiki: S. pneumoniae). The most common adverse experiences reported with PNEUMOVAX 23 in clinical trials were: Local reaction at injection site including soreness, erythema, warmth, swelling and induration, and fever <102°F. In post-marketing experience, injection site cellulitis-like reactions were reported rarely; between 1989 and 2002, when approximately 43 million doses were distributed, the annual reporting rate was <2/100,000 doses (Merck: PNEUMOVAX 23).

n. Human Response

Immune Response: It has been established that the purified pneumococcal capsular polysaccharides induce antibody production and that such antibody is effective in preventing pneumococcal disease (Merck: PNEUMOVAX 23).
Side Effects: Side effects of vaccination include: redness and soreness of the injection site, fever, and chills (Merck: PNEUMOVAX 23).
Efficacy: In many clinical studies on the efficacy of the vaccine, it has been found to have a 70-90% efficacy rate (FDA: PNEUMOVAX 23).

5. Prevnar

a. Product Name:

Pneumococcal 7-valent Conjugate Vaccine (Diphtheria CRM197 Protein)

b. Tradename:

Prevnar

c. Manufacturer:

Wyeth Pharmaceuticals Inc.

d. Vaccine Ontology ID:

VO_0000090

e. CDC CVX code:

100

f. Type:

Conjugate vaccine

g. Status:

Licensed

h. Location Licensed:

USA (License #0003)

i. Host Species for Licensed Use:

Human

j. Antigen

A sterile solution of saccharides of the capsular antigens of Streptococcus pneumoniae serotypes 4, 6B, 9V, 14, 18C, 19F, and 23F individually conjugated to diphtheria CRM197 protein (FDA: Prevnar).

k. Preparation

Each serotype is grown in soy peptone broth. The individual polysaccharides are purified through centrifugation, precipitation, ultrafiltration, and column chromatography.

l. Storage

DO NOT FREEZE. Store refrigerated at 2°C TO 8°C.

m . Approved Age for Licensed Use

2-15 months of age.

n. Contraindication

Hypersensitivity to any component of the vaccine, including diphtheria toxoid, is a contraindication to use of this vaccine.

o. Description

In the USA, a heptavalent pneumococcal conjugate vaccine vaccine (PCV 7) (e.g. Prevnar) is recommended since 2000 for all children aged 2–23 months and for at-risk children aged 24–59 months. The normally 4-doses series is given at 2, 4, 6 & 12–14 months of age. Protection is good against deep pneumococcal infections (especially septicemia and meningitis) (Wiki: S. pneumoniae).

p. Human Response

Vaccination Protocol: Efficacy was assessed in a randomized, double-blinded clinical trial in a multiethnic population at Northern California Kaiser Permanente (NCKP) from October 1995 through August 20, 1998, in which 37,816 infants were randomized to receive either Prevnar or a control vaccine at 2, 4, 6, and 12-15 months of age (FDA: Prevnar).
Immune Response: There were no reports of streptococcus pneumoniae in the group vaccinated with PREVNAR whereas in the control group, there were between 17-22 cases reported after vaccination (FDA: Prevnar).
Side Effects: Side effects of vaccination include: fever, irratibility, drowsiness, decreased appetite, rash and soreness/redness of the injection site.

6. Prevnar 13

a. Product Name:

Pneumococcal 13-valent Conjugate Vaccine [Diphtheria CRM197 Protein]

b. Tradename:

Prevnar

c. Manufacturer:

Wyeth Pharmaceuticals, Inc

d. Vaccine Ontology ID:

VO_0001244

e. CDC CVX code:

133

f. Type:

Conjugate vaccine

g. Status:

Licensed

h. Location Licensed:

USA, Canada

i. Host Species for Licensed Use:

Human

j. Adjuvant:

VO ID: VO_0000128

k. Immunization Route

Intramuscular injection (i.m.)

l. Storage

Store refrigerated at +2ºC to +8ºC (36ºF to 46ºF)

m . Approved Age for Licensed Use

Children 6 weeks through 5 years of age (FDA: Prevnar 13).

7. PsaA DNA Vaccine

a. Vaccine Ontology ID:

VO_0004517

b. Type:

DNA vaccine

c. Status:

Licensed

d. Gene Engineering of psaA

Type: DNA vaccine construction
Description:
Detailed Gene Information: Click here.

e. Vector:

pCI (Miyaji et al., 2001)

f. Immunization Route

Intramuscular injection (i.m.)

g. Mouse Response

Vaccination Protocol: Five male BALB/c mice were inoculated into each muscle tibialis anterior as previously described with 100 μl of 10 μM cardiotoxin and after 5 days with 25 μg of plasmid DNA in PBS (50 μl) (Miyaji et al., 2001).
Vaccine Immune Response Type: VO_0000286
Immune Response: Analysis of sera from mice immunized with purified PsaA showed the induction of mostly IgG1, that is, a Th2 like response. Furthermore, mice immunized with pCI-psaA developed readily detectable serum antibody titers 1 month after priming (Miyaji et al., 2001).
Efficacy: Immunization of mice with purified PsaA was shown to be protective against challenge with an heterologous S. pneumoniae strain (Miyaji et al., 2001).

8. S. pneumoniae 6PGD Protein Vaccine

a. Vaccine Ontology ID:

VO_0004069

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

Recombinant 6-Phosphogluconate dehydrogenase (6PGD) (Daniely et al., 2006).

e. Gene Engineering of Gnd

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

f. Adjuvant:

VO ID: VO_0000884
Description: Inject Alum adjuvant (Daniely et al., 2006).

g. Immunization Route

Intraperitoneal injection (i.p.)

h. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: Six-week-old BALB/c female mice were immunized intraperitoneally with 25 µg of r6PGD and 75 µl of Inject Alum adjuvant on days 0 (primary immunization) and 21 (booster). Control mice were sham-immunized with adjuvant only (Daniely et al., 2006).
Challenge Protocol: For respiratory challenge r6PGD immunized and control mice were anaesthetized with pentobarbital sodium (0·6 mg/kg) and inoculated intranasally with 1 × 10^8 S. pneumoniae strain WU2 (in 25 µl PBS). This inoculum's size was used as it was found to be the lowest that causes 100% mortality in our mouse model system within 96 h. Survival was monitored daily (Daniely et al., 2006).
Efficacy: Immunization of mice with r6PGD protected 60% (P < 0.001) of mice for 5 days and 40% (P < 0.05) of the mice for 21 days following intranasal lethal challenge (Daniely et al., 2006).

9. S. pneumoniae CbpA Protein Vaccine

a. Vaccine Ontology ID:

VO_0004005

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

N-terminal fragment of choline binding protein A (CbpA) (Ogunniyi et al., 2001).

e. Gene Engineering of CbpA

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

f. Adjuvant:

VO ID: VO_0001250
Description: Monophospholipid A (MPL) and aluminium phosphate (AlPO4) (Ogunniyi et al., 2001).

g. Adjuvant:

VO ID: VO_0000128
Description: Monophospholipid A (MPL) and aluminium phosphate (AlPO4) (Ogunniyi et al., 2001).

h. Immunization Route

Intraperitoneal injection (i.p.)

i. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: 5- to 6-week-old male BALB/c mice (13 to 15 per group) were immunized intraperitoneally with either adjuvant alone, adjuvant plus CbpA, or CbpA alone. Each mouse received three doses of 10 μg of each protein antigen in either formulation (AlPO4 or MPL plus AlPO4) at 12- to 14-day intervals (Ogunniyi et al., 2001).
Challenge Protocol: Intraperitoneal-challenge experiments were carried out 2 weeks after the third immunization using a highly virulent capsular type 2 strain (D39). Groups of immunized BALB/c mice were infected with either 1.3 × 10^5 or 1.3 × 10^7 CFU of the challenge strain. The challenge dose was equivalent to approximately 10^3 or 10^5 times the 50% lethal dose (LD50), respectively, for BALB/c mice. The mice were closely monitored for 21 days, and the survival time of each mouse was recorded (Ogunniyi et al., 2001).
Efficacy: CbpA was able to protect mice from intraperitoneal challenge with medium to very high doses of a highly virulent capsular type 2 pneumococcal strain, D39 (Ogunniyi et al., 2001).

10. S. pneumoniae ClpP protein Vaccine

a. Vaccine Ontology ID:

VO_0004006

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

ATP-dependent caseinolytic protease (ClpP)

e. Gene Engineering of ClpP

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

f. Adjuvant:

VO ID: VO_0000127
Description: Imject Alum no. 77161

g. Immunization Route

Intraperitoneal injection (i.p.)

h. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: Female BALB/c mice, weighing 16 to 18 g, were immunized three times at 14-day intervals with 10 µg of protein in alum adjuvant (3:1 [vol/vol]) of either ClpP protein in alum adjuvant or control protein in alum. Mice were boosted intraperitoneally with the same doses on days 14 and 28 (Wu et al., 2010).
Challenge Protocol: For the intraperitoneal challenge models, at 2 weeks after the last immunization BALB/c mice were administrated the virulent pneumococcal strain D39 at 1,500 times the 50% lethal dose (LD50; 1.5 x 10^5 CFU) or 150 times the LD50 (2.0 x 10^4 CFU). In the focal pneumonia models, BALB/c mice were intranasally inoculated with pneumococcal strain 31614 (serotype 14, 1.5 x 10^6 CFU) or 31693 (serotype 19F, 10^7 CFU). For intranasal challenge with pneumococcal strain D39, mice inspired the virulent pneumococcal strain D39 at 104 (7.5 x 10^7 CFU) or 103 (6.0 x 10^6 CFU) times the LD50 in a volume of 40 µl under injection anesthesia (Wu et al., 2010).
Efficacy: Compared to the control protein, ClpP reduced the median bacterial load nearly 100-fold in the focal pneumonia model (Wu et al., 2010).

11. S. pneumoniae detoxified PlyD1 Protein Vaccine

a. Type:

Recombinant vector vaccine

b. Status:

Licensed

c. Host Species for Licensed Use:

Baboon

d. Gene Engineering of PlyD1

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

e. Preparation

Recombinant PLY was produced from Escherichia coli containing the entire gene sequence of wild-type pneumolysin from strain R36A; the protein was column purified. PlyD1, a highly detoxified PLY variant, was generated by site-directed mutagenesis and differs from the wild-type form by three amino acid substitutions of T65C, G293C, and C428A. Recombinant PlyD1 protein was expressed in E. coli as soluble protein and column purified.(Salha et al., 2012)

f. Immunization Route

Intramuscular injection (i.m.)

12. S. pneumoniae DnaJ Protein Vaccine

a. Type:

Recombinant vector vaccine

b. Status:

Research

c. Host Species for Licensed Use:

Baboon

d. Host Species as Laboratory Animal Model:

Mouse

e. Gene Engineering of DnaJ

Type: Recombinant protein preparation
Description: PCR amplified DnaJ was ligated in pQE-30 expression vector and subsequently transformed in E. coli DH5alpha strain.(Khan et al., 2006)
Detailed Gene Information: Click here.

f. Preparation

PCR amplified DnaJ was ligated in pQE-30 expression vector and subsequently transformed in E. coli DH5alpha strain.(Khan et al., 2006)

g. Immunization Route

Intramuscular injection (i.m.)

13. S. pneumoniae GtS Protein Vaccine

a. Vaccine Ontology ID:

VO_0004068

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

recombinant (r) Glutamyl tRNA synthetase (GtS) (Mizrachi et al., 2007).

e. Gene Engineering of GltX

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

f. Adjuvant:

VO ID: VO_0000884
Description: Imject Alum adjuvant (Mizrachi et al., 2007).

g. Immunization Route

Intraperitoneal injection (i.p.)

h. Mouse Response

Host Strain: BALB/c OlaHsd
Vaccination Protocol: Mice were immunized intraperitoneally with 25 μg of rGtS and 75 μL of Imject Alum adjuvant on days 0 and 21. Control mice were sham immunized with adjuvant only (Mizrachi et al., 2007).
Challenge Protocol: For lethal respiratory challenge with S. pneumoniae, rGtS‐immunized and control mice were anesthetized with isoflurane and inoculated intranasally with S. pneumoniae serotype 3 strain WU2 (in 25 μL of PBS). The size of this inoculum was found to be the lowest that causes 100% mortality in control mice within 96 h. Survival was monitored daily (Mizrachi et al., 2007).
Efficacy: Thirty-nine percent of rGtS-immunized mice survived a lethal bacterial challenge, whereas no control mice survived (Mizrachi et al., 2007).

14. S. pneumoniae PhpA-79 Protein Vaccine

a. Vaccine Ontology ID:

VO_0004063

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

Recombinant PhpA-79

e. Gene Engineering of phpA

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

f. Adjuvant:

VO ID: VO_0001250
Description: 100 μg of monophosphoryl lipid A (MPL; Corixa, Hamilton, Mont.) per dose (Zhang et al., 2001).

g. Immunization Route

subcutaneous injection

h. Mouse Response

Host Strain: CBA/N
Vaccination Protocol: CBA/N mice, at 10 animals per group, were immunized with 5 μg of either rPhpA-79 or rPhpA-20. For parenteral immunization, rPhpA-79 or rPhpA-20 protein was mixed with 100 μg of monophosphoryl lipid A (MPL; Corixa, Hamilton, Mont.) per dose to a final volume of 200 μl in saline and then injected s.c. into mice. All groups received a booster with the same dose and by the same route 3 and 5 weeks after the primary immunization. Control mice were injected with MPL alone (Zhang et al., 2001).
Challenge Protocol: Mice were challenged with either serotype 3 or serotype 14 streptomycin-resistant S. pneumoniae (Zhang et al., 2001).
Efficacy: Immunization with either rPhpA-79–MPL provided 100% protection against death caused by type 3 S. pneumoniae infection (Zhang et al., 2001).

15. S. pneumoniae PiaA Protein Vaccine

a. Vaccine Ontology ID:

VO_0004070

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

Recombinant PiaA protein (Brown et al., 2001).

e. Gene Engineering of PiaA

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

f. Adjuvant:

VO ID: VO_0000884
Description: Imject Alum No. 77161

g. Immunization Route

Intraperitoneal injection (i.p.)

h. Mouse Response

Host Strain: BALB/c
Vaccination Protocol: For the active protection experiments, each mouse was immunized by intraperitoneal (i.p.) injection of 100 μl of each protein preparation in alum adjuvant on days 0, 10, and 20 (Brown et al., 2001).
Challenge Protocol: On day 34 mice were challenged i.p. with ca. 10^6 CFU of S. pneumoniae strain D39 organisms (Brown et al., 2001).
Efficacy: After 14 days the mortality of the group of mice immunized PiuA was 52%, showing that immunization with these proteins protects against systemic infection with S. pneumoniae (Brown et al., 2001).

16. S. pneumoniae PspA Protein Vaccine

a. Vaccine Ontology ID:

VO_0004007

b. Type:

Subunit vaccine

c. Status:

Research

d. Gene Engineering of PspA

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

e. Adjuvant:

VO ID: VO_0000884

f. Immunization Route

Intraperitoneal injection (i.p.)

g. Mouse Response

Host Strain: BALB/c and CD1
Vaccination Protocol: The mice received three doses of 10 μg of each antigen alone (or in combination) in 100 μg of alum adjuvant via Intraperitoneal injection (i.p.) at 14-day intervals or a placebo (Ogunniyi et al., 2007).
Challenge Protocol: Mice were challenged i.p. 2 weeks after the third immunization with either the highly virulent capsular type 2 strain D39 or a serotype 6A (WCH16) strain (Ogunniyi et al., 2007).
Efficacy: PspA elicited protection by using moderate challenge doses of S. pneumoniae (Ogunniyi et al., 2007).

17. S. pneumoniae pspA-rBCG Vector Vaccine

a. Vaccine Ontology ID:

VO_0004601

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Host Species as Laboratory Animal Model:

Mouse

e. Antigen

Pneumococcal surface protein A (PspA) present on all strains of pneumococci (Langermann et al., 1994)

f. Gene Engineering of PspA

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

g. Vector:

recombinant Bacille Calmette-Guerin (rBCG) (Langermann et al., 1994)

h. Immunization Route

Intramuscular injection (i.m.)

i. Description

Streptococcus pneumoniae is one of the leading causes of bacterial pneumonia and acute otitis media, a type of meningitis. It is becoming increasingly antibiotic resistant and the current available vaccine does not elicit protection in children. (Langermann et al., 1994)

j. Mouse Response

Host Strain: BALB/c and C3H/HeJ mice
Vaccination Protocol: A single intraperitoneal inoculation with a live BCG bacterial vaccine expressing PspA as a secreted mature protein in or a chimeric exported lipoprotein (rBCG-PspA), followed by a booster at 17 weeks(Langermann et al., 1994)
Vaccine Immune Response Type: VO_0003057
Challenge Protocol: BALB/c and C3H/HeJ mice immunized with rBCG-PspA vaccine strains were challenged intraperitoneally with 10^4 CFU of the highly lethal S. pneumoniae strain WU2 (Langermann et al., 1994)

Challenge Detail

No.	Pathogen Name	Dose	Route	Age	Interval
1	S. pneumoniae strain WU2	10000 CFU in volume ml	Intraperitoneal injection (i.p.)	day	day

Efficacy: Mice experienced passive protection with the use of rBCG against lethal challenge with the highly virulent, encapsulated WU2 strain of Streptococcus pneumoniae and two additional virulent strains exhibiting heterologous PspA and capsular sero- types(Langermann et al., 1994)

18. S. pneumoniae RASV synthesizing PsaA

a. Vaccine Ontology ID:

VO_0004071

b. Type:

Recombinant vector vaccine

c. Status:

Research

d. Gene Engineering of psaA

Type: Recombinant vector construction
Description:
Detailed Gene Information: Click here.

e. Vector:

Attenuated Salmonella enterica serovar Typhimurium strain RASV (Wang et al., 2010).

f. Immunization Route

Intranasally

g. Mouse Response

Host Strain: BALB/c, C57BL/6J
Vaccination Protocol: Mice were inoculated intranasally with 10 µl or orally with 20 µl of BSG containing 1 x 10^9 CFU of the RASV or control strain. In some experiments, the mice were boosted at week 6 with the same dose by using the same route as that used for primary immunization (Wang et al., 2010).
Challenge Protocol: At week 10, mice were challenged either by intraperitoneal injection with 2 x 10^4 CFU of S. pneumoniae WU2 (equal to 100x the 50% lethal dose [LD50]) or intranasally with 20 µl containing 5 x 10^6 CFU S. pneumoniae strain L82016 or E134 or 1 x 10^7 CFU of strain A66.1 or D39 (Wang et al., 2010).
Efficacy: BALB/c (haplotype H2(d)) or C57BL/6 (haplotype H2(b)) mice vaccinated either orally or intranasally exhibited a significant reduction in colonization of nasopharyngeal tissues after intranasal challenge with S. pneumoniae strains compared to controls, although protection was not observed with all challenge strains (Wang et al., 2010).

19. S. pneumoniae SrtA Protein Vaccine

a. Vaccine Ontology ID:

VO_0004008

b. Type:

Subunit vaccine

c. Status:

Research

d. Antigen

Recombinant sortase A (SrtA) (Gianfaldoni et al., 2009).

e. Gene Engineering of SrtA

Type: Recombinant protein preparation
Description:
Detailed Gene Information: Click here.

f. Adjuvant:

VO ID: VO_0000133

g. Immunization Route

Intraperitoneal injection (i.p.)

h. Mouse Response

Host Strain: BALB/c mice
Vaccination Protocol: Specific-pathogen-free BALB/c mice (Charles River) received three intraperitoneal administrations, 2 weeks apart, of 20 μg of SrtA along with Freund's adjuvant. Controls received the same course of saline plus adjuvant (Gianfaldoni et al., 2009).
Challenge Protocol: Three weeks after the last immunization, the animals were challenged intraperitoneally with one of the following S. pneumoniae strains: TIGR4, 1.4 × 10^2 CFU/mouse; D39, 10^3 CFU/mouse; or 35B-SME15, 7 × 10^3 CFU/mouse (Gianfaldoni et al., 2009).
Efficacy: Intraperitoneal immunization with recombinant SrtA was found to have conferred to mice protection against S. pneumoniae intraperitoneal challenge and the passive transfer of immune serum before intraperitoneal challenge was also protective. Moreover, by using the intranasal challenge model, significant reduction of bacteremia when mice were intraperitoneally immunized with SrtA was observed, while a moderate decrease of lung infection was achieved by intranasal immunization, even though no influence on nasopharynx colonization was seen (Gianfaldoni et al., 2009).

20. Synflorix

a. Product Name:

Pneumococcal conjugate vaccine (Non-Typeable Haemophilus influenzae (NTHi) protein D, diphtheria or tetanus toxoid conjugates) adsorbed

b. Tradename:

Synflorix

c. Manufacturer:

GlaxoSmithKline Inc.

d. Vaccine Ontology ID:

VO_0001245

e. Type:

Conjugate vaccine

f. Status:

Licensed

g. Location Licensed:

Canada

h. Host Species for Licensed Use:

Human

i. Antigen

Pneumococcal polysaccharides of serotypes 1,4,5,6B,7F,9V,14,18C,19F,23F (GSK: Synflorix).

j. Adjuvant:

VO ID: VO_0000128

k. Immunization Route

Intramuscular injection (i.m.)

l. Storage

Store in a refrigerator (2°C - 8°C).

m . Approved Age for Licensed Use

Infants and children from 6 weeks up to 2 years of age (GSK: Synflorix).

n. Description

SYNFLORIX™ [(pneumococcal conjugate vaccine (Non-Typeable Haemophilus influenzae (NTHi) protein D, diphtheria or tetanus toxoid conjugates) adsorbed] is a 10- valent pneumococcal polysaccharide conjugate vaccine using protein D derived from Non-Typeable Haemophilus influenzae as a carrier protein for 8 out of the 10 serotypes (1, 4, 5, 6B, 7F, 9V, 14 and 23F). Serotypes 18C and 19F are conjugated to tetanus toxoid and to diphtheria toxoid, respectively. All conjugates are adsorbed onto aluminum phosphate (GSK: Synflorix).

V. References

1. Anderson et al., 2016: Anderson RJ, Guru S, Weeratna R, Makinen S, Falconer DJ, Sheppard NC, Lang S, Chang B, Goenaga AL, Green BA, Merson JR, Gracheck SJ, Eyles JE. In vivo screen of genetically conserved Streptococcus pneumoniae proteins for protective immunogenicity. Vaccine. 2016; 34(50); 6292-6300. [PubMed: 27816374].

2. Brown et al., 2001: Brown JS, Ogunniyi AD, Woodrow MC, Holden DW, Paton JC. Immunization with components of two iron uptake ABC transporters protects mice against systemic Streptococcus pneumoniae infection. Infection and immunity. 2001; 69(11); 6702-6706. [PubMed: 11598041].

3. Chen et al., 2015: Chen A, Mann B, Gao G, Heath R, King J, Maissoneuve J, Alderson M, Tate A, Hollingshead SK, Tweten RK, Briles DE, Tuomanen EI, Paton JC. Multivalent Pneumococcal Protein Vaccines Comprising Pneumolysoid with Epitopes/Fragments of CbpA and/or PspA Elicit Strong and Broad Protection. Clinical and vaccine immunology : CVI. 2015; 22(10); 1079-1089. [PubMed: 26245351].

4. Daniely et al., 2006: Daniely D, Portnoi M, Shagan M, Porgador A, Givon-Lavi N, Ling E, Dagan R, Mizrachi Nebenzahl Y. Pneumococcal 6-phosphogluconate-dehydrogenase, a putative adhesin, induces protective immune response in mice. Clinical and experimental immunology. 2006; 144(2); 254-263. [PubMed: 16634799].

5. FDA: PNEUMOVAX 23: FDA: PNEUMOVAX 23 Vaccine for Streptococcus pneumoniae [http://www.fda.gov/BiologicsBloodVaccines/Vaccines/ApprovedProducts/ucm094055.htm]

6. FDA: Prevnar: FDA: Pneumococcal 7-valent Conjugate Vaccine [http://www.fda.gov/BiologicsBloodVaccines/Vaccines/ApprovedProducts/ucm094057.htm]

7. FDA: Prevnar 13: FDA: Prevnar 13 [http://www.fda.gov/BiologicsBloodVaccines/Vaccines/ApprovedProducts/ucm201667.htm]

8. Ferreira et al., 2006: Ferreira DM, Miyaji EN, Oliveira ML, Darrieux M, ArÃªas AP, Ho PL, Leite LC. DNA vaccines expressing pneumococcal surface protein A (PspA) elicit protection levels comparable to recombinant protein. Journal of medical microbiology. 2006; 55(Pt 4); 375-378. [PubMed: 16533983].

9. Gianfaldoni et al., 2009: Gianfaldoni C, Maccari S, Pancotto L, Rossi G, Hilleringmann M, Pansegrau W, Sinisi A, Moschioni M, Masignani V, Rappuoli R, Del Giudice G, Ruggiero P. Sortase A confers protection against Streptococcus pneumoniae in mice. Infection and immunity. 2009; 77(7); 2957-2961. [PubMed: 19433540].

10. Gonz�lez-Miro et al., 2017: Gonz�lez-Miro M, Rodr�guez-Noda L, Fari�as-Medina M, Garc�a-Rivera D, V�rez-Bencomo V, Rehm BHA. Self-assembled particulate PsaA as vaccine against <i>Streptococcus pneumoniae</i> infection. Heliyon. 2017; 3(4); e00291. [PubMed: 28435909].

11. GSK: Synflorix: GSK: Synflorix [http://www.gsk.ca/english/docs-pdf/Synflorix_PM_20090505_EN.pdf]

12. Jakobsen et al., 1999: Jakobsen H, Schulz D, Pizza M, Rappuoli R, Jónsdóttir I. Intranasal immunization with pneumococcal polysaccharide conjugate vaccines with nontoxic mutants of Escherichia coli heat-labile enterotoxins as adjuvants protects mice against invasive pneumococcal infections. Infection and immunity. 1999; 67(11); 5892-5897. [PubMed: 10531245].

13. Jang et al., 2017: Jang AY, Seo HS, Lin S, Chung GH, Kim HW, Lim S, Zhao L, Park IH, Lim JH, Kim KH. Molecular characterization of pneumococcal surface protein K, a potential pneumococcal vaccine antigen. Virulence. 2017; 8(6); 875-890. [PubMed: 28059611].

14. Khan et al., 2006: Khan MN, Bansal A, Shukla D, Paliwal P, Sarada SK, Mustoori SR, Banerjee PK. Immunogenicity and protective efficacy of DnaJ (hsp40) of Streptococcus pneumoniae against lethal infection in mice. Vaccine. 2006; 24(37-39); 6225-6231. [PubMed: 16797798].

15. Langermann et al., 1994: Langermann S, Palaszynski SR, Burlein JE, Koenig S, Hanson MS, Briles DE, Stover CK. Protective humoral response against pneumococcal infection in mice elicited by recombinant bacille Calmette-GuÃ©rin vaccines expressing pneumococcal surface protein A. The Journal of experimental medicine. 1994; 180(6); 2277-2286. [PubMed: 7964500].

16. McNeil et al., 2005: McNeil SA, Halperin SA, Langley JM, Smith B, Warren A, Sharratt GP, Baxendale DM, Reddish MA, Hu MC, Stroop SD, Linden J, Fries LF, Vink PE, Dale JB. Safety and immunogenicity of 26-valent group a streptococcus vaccine in healthy adult volunteers. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 2005; 41(8); 1114-1122. [PubMed: 16163629].

17. Merck: PNEUMOVAX 23: Merck: PNEUMOVAX 23 [http://www.merckvaccines.com/vaccines/pneu/index.html]

18. Miyaji et al., 2001: Miyaji EN, Dias WO, Gamberini M, Gebara VC, Schenkman RP, Wild J, Riedl P, Reimann J, Schirmbeck R, Leite LC. PsaA (pneumococcal surface adhesin A) and PspA (pneumococcal surface protein A) DNA vaccines induce humoral and cellular immune responses against Streptococcus pneumoniae. Vaccine. 2001; 20(5-6); 805-812. [PubMed: 11738744].

19. Mizrachi et al., 2007: Mizrachi Nebenzahl Y, Bernstein A, Portnoi M, Shagan M, Rom S, Porgador A, Dagan R. Streptococcus pneumoniae surface-exposed glutamyl tRNA synthetase, a putative adhesin, is able to induce a partially protective immune response in mice. The Journal of infectious diseases. 2007; 196(6); 945-953. [PubMed: 17703427].

20. Morsczeck et al., 2008: Morsczeck C, Prokhorova T, Sigh J, Pfeiffer M, Bille-Nielsen M, Petersen J, Boysen A, Kofoed T, Frimodt-Møller N, Nyborg-Nielsen P, Schrotz-King P. Streptococcus pneumoniae: proteomics of surface proteins for vaccine development. Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases. 2008; 14(1); 74-81. [PubMed: 18034862].

21. Ogunniyi et al., 2001: Ogunniyi AD, Woodrow MC, Poolman JT, Paton JC. Protection against Streptococcus pneumoniae elicited by immunization with pneumolysin and CbpA. Infection and immunity. 2001; 69(10); 5997-6003. [PubMed: 11553536].

22. Ogunniyi et al., 2007: Ogunniyi AD, Grabowicz M, Briles DE, Cook J, Paton JC. Development of a vaccine against invasive pneumococcal disease based on combinations of virulence proteins of Streptococcus pneumoniae. Infection and immunity. 2007; 75(1); 350-357. [PubMed: 17088353].

23. Ortqvist et al., 2005: Ortqvist A, Hedlund J, Kalin M. Streptococcus pneumoniae: epidemiology, risk factors, and clinical features. Seminars in respiratory and critical care medicine. 2005; 26(6); 563-574. [PubMed: 16388428].

24. Roche et al., 2007: Roche AM, King SJ, Weiser JN. Live attenuated Streptococcus pneumoniae strains induce serotype-independent mucosal and systemic protection in mice. Infection and immunity. 2007; 75(5); 2469-2475. [PubMed: 17339359].

25. Salha et al., 2012: Salha D, Szeto J, Myers L, Claus C, Sheung A, Tang M, Ljutic B, Hanwell D, Ogilvie K, Ming M, Messham B, van den Dobbelsteen G, Hopfer R, Ochs MM, Gallichan S. Neutralizing antibodies elicited by a novel detoxified pneumolysin derivative, PlyD1, provide protection against both pneumococcal infection and lung injury. Infection and immunity. 2012; 80(6); 2212-2220. [PubMed: 22473606].

26. Shi et al., 2010: Shi H, Wang S, Roland KL, Gunn BM, Curtiss R 3rd. Immunogenicity of a live recombinant Salmonella enterica serovar typhimurium vaccine expressing pspA in neonates and infant mice born from naive and immunized mothers. Clinical and vaccine immunology : CVI. 2010; 17(3); 363-371. [PubMed: 20053873].

27. Shivshankar et al., 2009: Shivshankar P, Sanchez C, Rose LF, Orihuela CJ. The Streptococcus pneumoniae adhesin PsrP binds to Keratin 10 on lung cells. Molecular microbiology. 2009; 73(4); 663-679. [PubMed: 19627498].

28. Textbook of Bacteriology: Online Textbook of Bacteriology: Streptococcus pneumoniae [http://textbookofbacteriology.net/S.pneumoniae.html]

29. Tostes et al., 2017: Tostes RO, Rodrigues TC, da Silva JB, Schanoski AS, Oliveira ML, Miyaji EN. Protection Elicited by Nasal Immunization with Recombinant Pneumococcal Surface Protein A (rPspA) Adjuvanted with Whole-Cell Pertussis Vaccine (wP) against Co-Colonization of Mice with Streptococcus pneumoniae. PloS one. 2017; 12(1); e0170157. [PubMed: 28103277].

30. Vadesilho et al., 2012: Vadesilho CF, Ferreira DM, Moreno AT, Chavez-Olortegui C, Machado de Avila RA, Oliveira ML, Ho PL, Miyaji EN. Characterization of the antibody response elicited by immunization with pneumococcal surface protein A (PspA) as recombinant protein or DNA vaccine and analysis of protection against an intranasal lethal challenge with Streptococcus pneumoniae. Microbial pathogenesis. 2012; 53(5-6); 243-249. [PubMed: 22981893].

31. Wang et al., 2010: Wang S, Li Y, Shi H, Scarpellini G, Torres-Escobar A, Roland KL, Curtiss R 3rd. Immune responses to recombinant pneumococcal PsaA antigen delivered by a live attenuated Salmonella vaccine. Infection and immunity. 2010; 78(7); 3258-3271. [PubMed: 20479086].

32. Wiki: S. pneumoniae: Wikipedia: Streptococcus pneumoniae [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae]

33. Wu et al., 2010: Wu K, Zhang X, Shi J, Li N, Li D, Luo M, Cao J, Yin N, Wang H, Xu W, He Y, Yin Y. Immunization with a combination of three pneumococcal proteins confers additive and broad protection against Streptococcus pneumoniae Infections in Mice. Infection and immunity. 2010; 78(3); 1276-1283. [PubMed: 20038538].

34. Xu et al., 2017: Xu Q, Pryharski K, Pichichero ME. Trivalent pneumococcal protein vaccine protects against experimental acute otitis media caused by Streptococcus pneumoniae in an infant murine model. Vaccine. 2017; 35(2); 337-344. [PubMed: 27919628].

35. Zhang et al., 2001: Zhang Y, Masi AW, Barniak V, Mountzouros K, Hostetter MK, Green BA. Recombinant PhpA protein, a unique histidine motif-containing protein from Streptococcus pneumoniae, protects mice against intranasal pneumococcal challenge. Infection and immunity. 2001; 69(6); 3827-3836. [PubMed: 11349048].

36. Zhang et al., 2017: Zhang J, Cui YL, Jiang YM. [Immunoprotective effect of combined pneumococcal endopeptidase O and pneumococcal surface adhesin A vaccines against Streptococcus pneumoniae infection]. Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics. 2017; 19(5); 583-589. [PubMed: 28506354].